Multipurpose cell experiment culture device
By designing a multi-purpose cell culture device made of silicone, the problems of inconsistent manual streaking and inconvenient co-culture operations in cell scratch experiments have been solved. This device achieves standardized and highly accurate cell scratch experiments and efficient co-culture functions, thereby reducing experimental costs.
Patent Information
- Application Number
- CN202423233683.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-26
AI Technical Summary
In existing cell scratch experiments, the precision of manual streaking is inconsistent, leading to inaccurate data. Furthermore, cell scratching and co-culture experiments need to be performed in different containers, which is inconvenient.
A multi-purpose cell culture device was designed, with a silicone body including an outer frame and a scratch partition. It can realize standardized cell scratch experiments and support cell co-culture. The experimental chamber is divided into independent chambers by the scratch partition and longitudinal partition, ensuring accuracy and flexibility.
It achieves standardization and accuracy in cell scratch assays, supports co-culture, improves experimental efficiency, reduces consumable costs, and the device is reusable.
Smart Images
Figure CN223705608U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to biological experiment equipment technical field, concretely relates to a multipurpose cell experiment culture device. BACKGROUND
[0002] Cell scratch experiment is a simple and effective in vitro experimental method for studying cell migration and repair capacity. On the cultured monolayer cells, a "scratch" is artificially made to form a cell-free blank area. After that, the process of cells migrating to the scratch area and gradually covering the blank area within a certain time is observed. This experiment can directly reflect the migration rate of cells, and by measuring the healing degree of the scratch at different time points, the movement ability of cells can be evaluated, and the influence of different factors (such as drugs, gene expression, etc.) on cell migration behavior can be studied, which provides important information for the research in the fields of cell biology, pathology and pharmacology.
[0003] In some studies, cell scratch experiment can be combined with co-culture experiment. For example, in the co-culture system, one of the cells is scratched, and the change of its migration and repair capacity under the condition of co-culture with other cells is observed to explore the influence of co-cultured cells on its migration behavior.
[0004] However, the existing technology has the following disadvantages:
[0005] (1) In the existing cell scratch experiment, the user usually uses a suction head to draw a line on the cell group on the slide, but due to the limited accuracy of the width and length of the artificial line, the standard is not uniform, and the data is not rigorous.
[0006] (2) The past cell co-culture and scratch experiment need to be realized in different vessels, which is relatively inconvenient.
[0007] Therefore, there is an urgent need for a device that can be reused for cell scratch experiment and cell co-culture. UTILITY MODEL CONTENT
[0008] In view of the deficiencies of the prior art, the utility model provides a multipurpose cell experiment culture device, which can realize standard unified cell scratch experiment and can be used for cell co-culture experiment.
[0009] The technical scheme of the utility model is as follows:
[0010] A multipurpose cell experiment culture device, comprising a device body, the device body comprising an outer frame and a scratch partition plate, the outer frame being closed to form an experimental cavity, the scratch partition plate being arranged in the middle of the experimental cavity and connected with the outer frame, the bottom of the scratch partition plate being flush with the bottom of the outer frame, and the height of the scratch partition plate being less than the height of the outer frame.
[0011] The further technical scheme of the embodiment is that the device body further comprises a plurality of longitudinal partition plates, the longitudinal partition plates are arranged in the experimental cavity perpendicularly to the scratch partition plate, the longitudinal partition plates are arranged at intervals and parallel to each other, the height of the longitudinal partition plate is greater than the height of the scratch partition plate, and the experimental cavity is divided into a plurality of independent chambers.
[0012] The further technical scheme of the embodiment is that the scratch partition plate comprises an integral forming scratch line part and a main body part, the scratch line part and the main body part are arranged in stack, the bottom surface of the scratch line part is arranged flush with the outer frame, and the width of the scratch line part is 100 um to 1000 um.
[0013] The further technical scheme of the embodiment is that the scratch partition plate 2 and / or the longitudinal partition plate can be detachably mounted in the experimental cavity.
[0014] The further technical scheme of the embodiment is that the bottom of the longitudinal partition plate is arranged flush with the outer frame, and the height of the longitudinal partition plate is less than the height of the outer frame.
[0015] The further technical scheme of the embodiment is that a plurality of cell respiration channels are arranged on the outer frame.
[0016] The further technical scheme of the embodiment is that the inner side wall of the outer frame is a round corner structure.
[0017] The further technical scheme of the embodiment is that a surrounding edge is arranged at the bottom of the outer frame, and the surrounding edge extends away from the outer side of the outer frame.
[0018] The further technical scheme of the embodiment is that the device body is an integral forming structure.
[0019] The further technical scheme of the embodiment is that the device body is made of silica gel.
[0020] Compared with the prior art, the utility model has the following advantages:
[0021] The utility model discloses in using, adds cell in the experimental cavity on the both sides of scratch partition plate, removes device body after cell full -grown experimental cavity, and the scratch is formed in the middle part of cell because of the block of scratch partition plate, thereby satisfies the scratch experimental standard of unity. Because the bottom of scratch partition plate is flush with the outer frame, and the height is lower than the outer frame, the experimental cavity on the both sides of scratch partition plate can be used for the co -culture experiment of different cells in the same culture medium respectively, so that device body is used for cell scratch experiment and co -culture experiment simultaneously. DRAWINGS
[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the description of the embodiments or the prior art. Obviously, the drawings described below only illustrate some of the embodiments of the present application, and other drawings can be obtained by those of ordinary skill in the art without creative effort based on these drawings.
[0023] Figure 1 Figure 1 is a perspective view of a multi-purpose cell experiment culture device according to the present application;
[0024] Figure 2 Figure 2 is another perspective view of the multi-purpose cell experiment culture device according to the present application; Figure 1 Figure 3 is a top view of the multi-purpose cell experiment culture device according to the present application;
[0025] Figure 3 Figure 4 is a sectional view of the multi-purpose cell experiment culture device according to the present application along A-A.
[0026] Figure 4 Figure 5 is a sectional view of the multi-purpose cell experiment culture device according to the present application along B-B. Figure 3
[0027] The drawings are identified as follows: 1-outer frame; 11-enclosure; 2-scratch partition; 21-scratch part; 22-main part; 3-longitudinal partition. DETAILED DESCRIPTION
[0028] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort fall within the scope of protection of the present application.
[0029] In the description of the present application, it should be noted that the orientations or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third", "fourth", etc. are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0030] In the description of the utility model, it needs to be explained that, unless there is explicit provision and limitation, the terms "mounting", "connection", "connection" should be understood in a broad sense, for example, it can be fixed connection, or it can be detachable connection, or it can be integrally connected; it can be mechanical connection, or it can be electrical connection; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication between two elements. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0031] In order to solve the problems in the background art, the commonly used plastic material chamber slide on the market is sealed by putting a silica gel pad at the bottom of the plastic material, then a fastener is added on the side to reinforce it and used for cell culture, and then used for observation imaging after removing the plastic material part. However, this structure is relatively complex, single function, and cannot be reused, resulting in high use cost and being not environmentally friendly.
[0032] Embodiment 1
[0033] As Figure 1 shown, the utility model discloses a multipurpose cell experiment culture device, including device body, device body includes outer frame 1 and scratch baffle 2, the outer frame 1 is closed and forms experiment cavity, and scratch baffle 2 is arranged in the middle part of experiment cavity and is connected with outer frame 1, and the bottom of scratch baffle 2 is flush with the bottom of outer frame 1, and the height of scratch baffle 2 is less than the height of outer frame 1. The device body is in the shape of "day", and the bottom of scratch baffle 2 is flush with the bottom of outer frame 1. During the culture of cell scratch experiment, the cells in the experiment cavities on both sides of scratch baffle 2 can be prevented from penetrating each other through the gap at the bottom of the device body, which can affect the experiment. Those skilled in the art can understand that, from the top view, the closed outer frame 1 can be square, circular or polygonal in structure.
[0034] The working principle of the device is as follows:
[0035] When cell scratch experiment is needed, the device body is arranged on the glass slide, and the same cells are inoculated on both sides of scratch baffle 2 in the experiment cavity for culture. After the culture is completed, the device body is removed, and the scratch caused by scratch baffle 2 in the middle of the two groups of cells can be observed for cell migration.
[0036] When cell co-culture experiment is needed, the device body is arranged on the glass slide, and different cells are inoculated on both sides of scratch baffle 2 in the experiment cavity, and the culture medium solution is higher than scratch baffle 2, so that the two groups of cells are in the same cell culture environment, thereby completing the cell co-culture experiment.
[0037] Specifically, the device body is integrally formed of silica gel material. Due to the characteristics of silica gel, the device body can be formed by an integral molding process, which can reduce the connection gap generated during assembly of parts and avoid interference with the experiment. The silica gel surface is more flat, which can be completely attached to the glass slide or culture dish for adsorption. After adsorption, there is no water leakage or air leakage, and after completing the culture, the scratch experiment observation can be easily removed without leaving marks, improving the efficiency of the experiment. The device body made of silica gel material can be directly placed in a 200-degree high-temperature oven for 4 hours for sterilization and removal of endotoxins, avoiding cell contamination during each experiment, so that the device can be reused multiple times.
[0038] Further, the scratch partition plate 2 includes an integrally formed scratch line part 21 and a main body part 22. The scratch line part 21 and the main body part 22 are stacked, and the bottom surface of the scratch line part 21 is flush with the outer frame 1. The width of the scratch line part 21 is 100um-1000um. After completing the culture of the cells, the width of the scratch partition plate 2 is the width of the cell scratch, ensuring the accuracy of the cell scratch experiment each time, and the silica gel material can ensure that the scratch is straight and has no burrs.
[0039] Further, the outer frame 1 is provided with a plurality of cell respiration channels. Through the cell respiration channels, the cells can still perform normal respiration after the cover is closed.
[0040] Optionally, the inner side wall of the outer frame 1 is a rounded structure. The rounded structure of the inner side wall can facilitate cleaning of the experimental cavity, avoiding contamination of cell solutions during different experiments.
[0041] The further technical solution of the embodiment is that the outer frame 1 is provided with a surrounding edge 11 extending away from the outer side of the outer frame 1. By providing the outwardly extending surrounding edge 11, the device body and the glass slide are more firmly attached.
[0042] Embodiment 2
[0043] As shown in Figures 1 to 4 The embodiment further includes a plurality of longitudinal partition plates 3, which are perpendicular to the scratch partition plate 2 and are arranged in the experimental cavity. The longitudinal partition plates 3 are arranged in parallel and spaced apart. The height of the longitudinal partition plate 3 is greater than the height of the scratch partition plate 2, which divides the experimental cavity into a plurality of independent chambers. In this embodiment, three longitudinal partition plates 3 are preferably provided, which divide the experimental cavity into four relatively independent large chambers. The scratch partition plate 2 arranged in the larger chamber further divides it into two smaller chambers. The four large chambers are relatively independent, which can simultaneously perform multiple scratch experiments or co-culture experiments on a single device, effectively improving the efficiency of the experiment and reducing the time for experimental preparation and operation.
[0044] Further, the bottom of the longitudinal partition plate 3 is flush with the outer frame 1, and the height of the longitudinal partition plate 3 is less than the height of the outer frame 1. The height of the longitudinal partition plate 3 is greater than the height of the scratch partition plate 2, which facilitates the operation of the experimenters and distinguishes different experimental chambers, so that the two cells in the co-culture experiment can be in the same culture medium solution environment and will not overflow the device.
[0045] Embodiment 3
[0046] The difference between this embodiment and embodiment 1 is that the scratch partition plate 2 and / or the longitudinal partition plate 3 can be detachably installed in the experimental chamber. Those skilled in the art can understand that the scratch partition plate 2 and the longitudinal partition plate 3 can be movably fixed in the experimental chamber through a clamping groove, adhesive or sliding groove, etc., and connected with the outer frame 1. The detachable scratch partition plate 2 and longitudinal partition plate 3 can adjust the size of the experimental chamber, more portable for cleaning the device, and improve the use flexibility of the device body. In addition, the split design can effectively reduce the production difficulty and improve the production efficiency of the device.
[0047] The beneficial effects of the utility model lie in:
[0048] 1. Adopting silica gel material, compared with traditional glass or plastic material, silica gel has better biocompatibility and chemical stability.
[0049] 2. High-precision interval design, the scratch width formed by the silica gel scratch partition plate is 100um-1000um:
[0050] 3. Precise control: high-precision interval ensures the accuracy and repeatability of the experiment, which is crucial for the reliability of cell culture and experimental results.
[0051] 4. Multifunctionality: cell scratch culture: breakthroughly supports cell scratch culture, which is an important experimental method for evaluating cell migration ability, and has important significance for studying cell behavior and disease models.
[0052] Co-culture function: retains and optimizes the co-culture function, allowing different types of cells to be cultured on the same slide, which is very valuable for studying cell-cell interactions and microenvironmental influences.
[0053] 5. Efficient experimental design:
[0054] Four groups of scratch experiments: one plug-in can perform four groups of scratch experiments, improving experimental efficiency and reducing experimental preparation and operation time.
[0055] 6. Reusability: due to the durability and cleanability of the silica gel material, the slide can be reused, reducing the cost of consumables in the laboratory.
[0056] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A multi-purpose cell culture apparatus, comprising an apparatus body, characterized in that, The device body includes an outer frame (1) and a scratching partition (2). The outer frame (1) is closed to form an experimental chamber. The scratching partition (2) is located in the middle of the experimental chamber and connected to the outer frame (1). The bottom of the scratching partition (2) is flush with the bottom of the outer frame (1) and the height of the scratching partition (2) is less than the height of the outer frame (1).
2. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The device body also includes several longitudinal partitions (3), which are arranged in the experimental chamber perpendicular to the scratch partition (2). The longitudinal partitions (3) are spaced apart and parallel to each other. The height of the longitudinal partitions (3) is greater than the height of the scratch partition (2), dividing the experimental chamber into several independent chambers.
3. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The scratch partition (2) includes an integrally formed scribing part (21) and a main body part (22). The scribing part (21) and the main body part (22) are stacked. The bottom surface of the scribing part (21) is flush with the outer frame (1). The width of the scribing part (21) is 100um to 1000um.
4. The multi-purpose cell culture apparatus according to claim 2, characterized in that, The scratch partition (2) and / or the longitudinal partition (3) are detachably installed in the experimental chamber.
5. The multi-purpose cell culture apparatus according to claim 2, characterized in that, The bottom of the longitudinal partition plate (3) is flush with the outer frame (1), and the height of the longitudinal partition plate (3) is less than the height of the outer frame (1).
6. The multi-purpose cell culture apparatus according to claim 1, characterized in that, Several cellular respiration channels are provided on the outer frame (1).
7. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The inner wall of the outer frame (1) has a rounded corner structure.
8. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The bottom of the outer frame (1) is provided with a rim (11), which extends outward away from the outer frame (1).
9. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The device body is a one-piece molded structure.
10. The multi-purpose cell culture apparatus according to claim 1, characterized in that, The main body of the device is made of silicone.