Miniaturized portable norovirus detection device

Abstract

The utility model relates to the technical field of virus detection, and discloses a miniaturized portable norovirus detection device which comprises a detection box, an anti-falling mechanism is detachably connected to the outer part of the detection box, a sealing mechanism is fixedly connected to the inner wall of the detection box, a detection mechanism is fixedly connected to the inner part of the sealing mechanism, and the inner part of the detection mechanism is fixedly connected with the anti-falling mechanism. The sealing mechanism comprises two sliding rails, the two sides of the two sliding rails are fixedly connected to the two sides of the inner wall of the detection box respectively, the close sides of the two sliding rails are slidably connected with an inner box, and the inner wall of the inner box is fixedly connected with a sealing film. According to the utility model, the whole detection device is subjected to double sealing before being used, so that external interfering substances are effectively prevented from entering the device, the stability and the purity of a detection reagent are ensured, and meanwhile, the risk that the detection mechanism is damaged due to unforeseen circumstances such as collision, shaking and the like in the transportation and storage processes is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to virus detection technical field especially relates to miniaturized portable norovirus detection device. BACKGROUND

[0002] Norovirus is a kind of virus that can cause acute gastroenteritis, can cause patients to appear vomiting, diarrhea, abdominal pain, fever and other discomfort symptoms, endanger human health, especially to children, the old and other weak immune groups influence is larger. It usually infects through the fecal-oral route, contacts contaminated food or water source, aerosol and other routes. The role of norovirus detection device is to quickly and accurately detect whether norovirus exists in the sample, and its types include immunochromatography test paper, enzyme-linked immunosorbent assay kit, real-time fluorescent quantitative PCR detection reagent, which helps to discover virus infection in time and take corresponding prevention and treatment measures.

[0003] The enzyme-linked immunosorbent assay kit in the miniaturized portable norovirus detection device usually includes a microplate, a solid phase carrier coated with norovirus specific antibodies, an enzyme-labeled detection antibody, a color developing substrate, a washing solution, a termination solution and the like. Its working principle is to add the sample to be detected to the solid phase carrier coated with specific antibodies. If norovirus antigen exists in the sample, it will bind to the solid phase antibody. Then the enzyme-labeled detection antibody is added to react with it. After that, the unbound substances are removed by washing. The color developing substrate is added. The color developing reaction occurs under the catalysis of the enzyme. Finally, the reaction is terminated by the termination solution. The result is determined by detecting the absorbance, so as to determine whether the sample contains norovirus.

[0004] In the prior art, the entire detection device will shake during transportation, which will cause damage to the outer packaging of the detection device, resulting in damage to the sealing property of the entire device, so that the detection reagent inside the entire detection device is easily contaminated by external substances, which will interfere with the normal combination of the detection reagent and the norovirus antigen, and reduce the detection accuracy. Therefore, the miniaturized portable norovirus detection device is proposed to solve the above problems. UTILITY MODEL CONTENT

[0005] In order to make up for the above shortcomings, the utility model provides a miniaturized portable norovirus detection device, which aims to improve the problem that the sealing property of part of the devices in the prior art is easily damaged, resulting in reduced detection accuracy.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] The application discloses a miniaturized portable norovirus detection device, which comprises a detection box, a falling-off prevention mechanism is detachably connected to the outside of the detection box, a sealing mechanism is fixedly connected to the inner wall of the detection box, and a detection mechanism is fixedly connected to the inside of the sealing mechanism.

[0008] The sealing mechanism comprises two sliding rails, the two sliding rails are fixedly connected to the inner walls of the detection box on the two sides respectively, and an inner box is slidingly connected to the proximal sides of the two sliding rails.

[0009] As a further description of the above technical scheme:

[0010] The falling-off prevention mechanism comprises a sealing cover, one end of the sealing cover is detachably connected to one end of the detection box, and a sawtooth-shaped sealing strip is arranged at the connecting position of the sealing cover and the detection box.

[0011] As a further description of the above technical scheme:

[0012] The detection mechanism comprises a bottom plate, the bottom of the bottom plate is fixedly connected to the inner wall at the bottom of the inner box, a sample feeding strip is fixedly connected to the top of the bottom plate, a conductive layer is fixedly connected to the top of the bottom plate, a detection layer is fixedly connected to the top of the bottom plate, and a guide layer is fixedly connected to the top of the bottom plate.

[0013] As a further description of the above technical scheme:

[0014] The two sliding rails are deformable, and the sealing film is a disposable polyethylene film.

[0015] As a further description of the above technical scheme:

[0016] The bottom of the sample feeding strip is in contact with the top of the conductive layer, and the bottoms of the proximal ends of the conductive layer and the guide layer are respectively in contact with the tops of the two ends of the conductive layer.

[0017] The application has the following beneficial effects:

[0018] 1、in the utility model, through pinching the sealing cover and pulling outwards, the detection box and the sealing cover are disconnected along the sawtooth-shaped sealing strip, then the inner box is moved outwards by pulling the handle on the inner box, and then the sealing film is torn, so that the detection device can be used, the whole detection device is double-sealed before use, external air, moisture and impurities are prevented from entering the inside of the device, the stability and purity of the detection reagent are ensured, deviation of the detection result caused by external factors is avoided, and the risk that the detection mechanism is damaged due to accidents such as collision and shaking during transportation and storage is reduced.

[0019] 2、The utility model discloses, through the sample drop into the sample bar, sample diffuses in the sample bar and enters the conduction layer, and the sample flows to the detection layer in the unidirectional of conduction layer and reacts, and the sample and the reagent in the detection layer react and form the color change, so that the user can conveniently intuitively detect whether the sample contains the virus, convenient operation reduces the requirement to the professional skill of operating personnel, makes detection more popular, strengthens the whole virus detection ability. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is the three-dimensional schematic view of the miniaturized portable norovirus detection device provided by the utility model;

[0021] Figure 2 It is the structure explosion map of the sealing cover of the miniaturized portable norovirus detection device provided by the utility model;

[0022] Figure 3 It is the structure schematic view of the inner box of the miniaturized portable norovirus detection device provided by the utility model;

[0023] Figure 4 It is Figure 3 The enlarged view of A in the middle.

[0024] Legend:

[0025] 1, detection box, 2, sealing cover, 3, sawtooth seal, 4, slide rail, 5, inner box, 6, sealing film, 7, bottom plate, 8, sample bar, 9, conduction layer, 10, detection layer, 11, guide layer. DETAILED DESCRIPTION

[0026] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0027] With reference to Figures 1 to 3 An embodiment provided by the utility model: miniaturized portable norovirus detection device, including detection box 1, detection box 1 as the core bearing part of whole device, provides stable installation foundation and protection space for other mechanisms, and the overall design volume of detection box 1 is smaller, convenient for user to carry. The outer portion of detection box 1 is detachably connected with the anti-falling mechanism, and the anti-falling mechanism can effectively prevent the detection reagent strip in the detection box 1 from falling due to accidental collision, shaking and the like. The inner wall of the detection box 1 is fixedly connected with the sealing mechanism, and the sealing mechanism is fixedly connected with the detection mechanism in the inside.

[0028] The sealing mechanism comprises two slide rails 4, which are deformable and fixedly connected to the inner walls of the detection box 1 on both sides. The deformable design of the two slide rails 4 enables them to better adapt to the slight dimensional deviations of the inner box 5 during installation, thereby ensuring the sealing of the connection between the slide rails 4 and the subsequent structure. At the same time, when the device is subjected to external force impact, the slide rails 4 can deform to buffer part of the energy, reducing the impact on the internal detection mechanism. The inner box 5 is slidably connected to the side of the two slide rails 4, and can smoothly enter and exit the detection box 1 along the slide rails 4, facilitating the placement and removal of the test reagent strip. The inner wall of the inner box 5 is fixedly connected with a sealing film 6, which is a disposable polyethylene film. The sealing film 6 can effectively prevent external air, moisture and other impurities from entering the inner box 5, thereby ensuring the purity and stability of the test reagent and avoiding deviations in the test results caused by external factors. The sealing film 6 made of polypropylene has high strength and toughness. It can withstand a certain degree of stretching and extrusion, and is not easy to break during transportation and storage of the reagent box.

[0029] The anti-falling mechanism comprises a sealing cover 2, which is detachably connected to one end of the detection box 1, thereby facilitating the opening and closing operations of the detection box 1 and facilitating the handling of the test reagent and sample in the inner box 5 by the operator. The connection between the sealing cover 2 and the detection box 1 is provided with a sawtooth seal 3, which enables the detection box 1 and the sealing cover 2 to be stably bonded together, ensuring the sealing of the connection between them, thereby preventing the automatic disconnection of the internal detection mechanism when the detection box 1 is shaken, and also preventing the sealing of the inside of the sealing box when not in use, and only needs to pinch the sealing cover 2 and pull it out when needed, and the detection device can be used by tearing the sawtooth seal 3.

[0030] Reference Figures 3 to 4The detection mechanism includes a bottom plate 7 fixedly connected to the bottom inner wall of the inner box 5 at the bottom of the bottom plate 7. The bottom plate 7 ensures the stability of the position of the detection mechanism in the detection box 1, avoiding the influence of the accuracy of the detection result caused by shaking or displacement during the detection process. The top of the bottom plate 7 is fixedly connected with a sample inlet strip 8. When the sample containing norovirus is dropped onto the sample inlet strip 8, due to the special material and surface treatment process of the sample inlet strip 8, the sample can quickly and uniformly spread on the surface thereof and penetrate downward along the sample inlet strip 8 under the action of capillary. The top of the bottom plate 7 is fixedly connected with a conductive layer 9, the bottom of the sample inlet strip 8 is in contact with the top of the conductive layer 9, and the conductive layer 9 is used for conducting the sample penetrating out of the sample inlet strip 8 to the subsequent structure for detection in one direction. The top of the bottom plate 7 is fixedly connected with a detection layer 10 containing specific detection reagents for norovirus. When the sample signal conducted by the conductive layer 9 reaches the detection layer 10, the reagents in the detection layer 10 will have specific immune response or nucleic acid hybridization reaction with norovirus, and according to the reaction result, a recognizable signal change (color change) is generated. The top of the bottom plate 7 is fixedly connected with a guide layer 11, the bottom of the proximal end of the conductive layer 9 is in contact with the top of the two ends of the conductive layer 9 respectively, and the guide layer 11 has an adsorption function, so that the sample can automatically spread when dropped into the sample inlet strip 8 and flow along the conductive layer 9 to the detection layer 10 to the guide layer 11 due to the adsorption capacity of the guide layer 11, thereby ensuring the one-way flow of the sample and the accuracy of the detection.

[0031] Working principle: when using the detection device, the detection box 1 and the sealing cover 2 are disconnected along the serrated seal 3 by pinching the sealing cover 2 and pulling it outward, so that the entire reagent box is opened, and then the inner box 5 is axially moved outward along the two slide rails 4 by pulling the handle on the inner box 5, and then the sample is dropped into the sample inlet strip 8 by tearing the sealing film 6, the sample spreads in the sample inlet strip 8 and enters the conductive layer 9 due to the guiding action of the guide layer 11, the sample flows in one direction to the detection layer 10 for reaction under the transmission action of the conductive layer 9, the sample reacts with the reagents in the detection layer 10 to form a color change, so that the user can intuitively feel the presence or absence of the virus in the sample through the color change of the detection layer 10.

[0032] During use, the design of the sealing cover 2 seals the contact position between the entire detection box 1 and the outside, thereby preventing the inner box 5 from automatically sliding out during transportation due to shaking, causing damage to the detection reagent strip. The design of the disposable sealing film 6 ensures that the entire detection reagent strip is not disturbed by external pollutants before use, and the disposable design reduces the cost of the entire detection device. Due to the small size and light weight of the entire detection box 1, it can be carried with you, meeting the market demand for miniaturization and portability.

[0033] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.

Claims

1. A miniaturized portable norovirus detection device comprising a detection cartridge (1), characterized in that: The detection box (1) is detachably connected with an anti-falling mechanism outside, the inner wall of the detection box (1) is fixedly connected with a sealing mechanism, and the sealing mechanism is fixedly connected with a detection mechanism inside; The sealing mechanism comprises two slide rails (4), the two sides of the two slide rails (4) are fixedly connected with the inner walls of the detection box (1) on the two sides, and the side close to the two slide rails (4) is slidably connected with an inner box (5); the inner wall of the inner box (5) is fixedly connected with a sealing film (6).

2. The miniaturized portable norovirus detection device of claim 1, wherein: The anti-falling mechanism comprises a sealing cover (2), one end of the sealing cover (2) is detachably connected with one end of the detection box (1), and a zigzag sealing strip (3) is arranged at the connection between the sealing cover (2) and the detection box (1).

3. The miniaturized portable norovirus detection device of claim 1, wherein: The detection mechanism comprises a bottom plate (7), the bottom of the bottom plate (7) is fixedly connected with the inner wall at the bottom of the inner box (5), the top of the bottom plate (7) is fixedly connected with a sample inlet strip (8), the top of the bottom plate (7) is fixedly connected with a conductive layer (9), the top of the bottom plate (7) is fixedly connected with a detection layer (10), and the top of the bottom plate (7) is fixedly connected with a guide layer (11).

4. The miniaturized portable norovirus detection device of claim 1, wherein: The two slide rails (4) are deformable, and the sealing film (6) is a disposable polyethylene film.

5. The miniaturized portable norovirus detection device of claim 3, wherein: The bottom of the sample inlet strip (8) is in contact with the top of the conductive layer (9), and the bottom of the end close to the conductive layer (9) and the guide layer (11) is in contact with the top of the two ends of the conductive layer (9) respectively.

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