Novel column chromatography device

Abstract

The utility model discloses a novel column chromatography device which comprises two support frames and a column chromatography body, the inner sides of the two support frames are respectively and rotatably connected with a short shaft, one ends of the two short shafts are respectively and fixedly provided with a telescopic frame, one side of each telescopic frame is respectively connected with two connecting shafts, the other ends of the two connecting shafts are respectively and fixedly provided with a clamping block, and the clamping blocks are respectively and fixedly connected with the column chromatography body. The column chromatography body is clamped among the plurality of clamping blocks, a rotating wheel is arranged on one side of the supporting frame, one end of the short shaft penetrates through the supporting frame and is fixedly connected with one side of the rotating wheel, a plurality of limiting grooves are formed in the rotating wheel, a limiting mechanism is arranged on one side of the supporting frame, and a threaded cylinder is fixedly arranged on the lower side of one supporting frame. One side of one supporting frame is rotatably connected with a threaded cylinder, one side of the other supporting frame is rotatably connected with a threaded rod, one end of the threaded rod is arranged in the threaded cylinder in a penetrating manner, one side of each supporting frame is provided with a rotating mechanism, and the rotating mechanisms are connected with the threaded rods.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of column chromatography, especially a novel column chromatography device. BACKGROUND

[0002] The column chromatography device is a kind of laboratory equipment for chemical separation and purification, usually used for separating and purifying biological macromolecules (such as proteins, nucleic acids, etc.) or small-molecule organic compounds. The column chromatography device is usually composed of a filler column, a sample inlet, an eluent inlet and outlet, etc. Different kinds of solid-phase fillers are filled in the filler column, and the sample is washed and separated by eluent in the filler column to achieve the purpose of separation and purification.

[0003] The existing column chromatography device installs the chromatographic column on the support frame, but usually only one kind of chromatographic column can be installed on one kind of support frame. In the column chromatography device, the angle of the chromatographic column on the support frame needs to be adjusted. The inclination angle of the chromatographic column can affect the flow rate and separation effect of the sample in the filler column, and can also affect the uniformity of the liquid phase in the filler column. However, the angle adjustment of the existing chromatographic column on the support frame is not convenient, and since only one kind of chromatographic column can be installed on one kind of support frame, the angle of different chromatographic columns cannot be adjusted. In view of the above problems, the following solution is proposed. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a novel column chromatography device, which has the advantages of being able to fix and install column chromatography of different sizes and being convenient for adjusting the angle of column chromatography.

[0005] The above technical purpose of the utility model is realized by the following technical scheme:

[0006] A novel column chromatography device, comprising two support frames and a column chromatography body, the inner sides of the two support frames are respectively rotationally connected with short shafts, one end of each of the two short shafts is fixedly provided with an extension frame, one side of each of the extension frames is respectively connected with two connecting shafts, the other end of each of the two connecting shafts is fixedly provided with a clamping block, and the column chromatography body is clamped between a plurality of clamping blocks, one side of the support frame is provided with a rotating wheel, one end of the short shaft penetrates the support frame and is fixedly connected with one side of the rotating wheel, a plurality of limiting grooves are arranged on the rotating wheel, one side of the support frame is provided with a limiting mechanism, and the limiting mechanism is matched with the limiting grooves, the lower side of one of the support frames is fixedly provided with a threaded cylinder, one side of the other support frame is rotationally connected with a threaded rod, one end of the threaded rod penetrates the threaded cylinder, one side of the support frame is provided with a rotating mechanism, and the rotating mechanism is connected with the threaded rod, the lower side of one of the support frames is fixedly provided with two guide cylinders, and the lower side of the other support frame is fixedly provided with two guide columns, one end of each of the two guide columns penetrates the guide cylinder.

[0007] As preferred, the telescopic frame is provided with a telescopic child rod and a telescopic parent rod, the lower end of the telescopic child rod is slidably embedded in the telescopic parent rod, one side of the telescopic parent rod is provided with a fixing pin, the telescopic child rod is provided with a plurality of fixing holes, the top end of the fixing pin is embedded in the fixing hole, a reset spring is embedded on the fixing pin, and the two ends of the reset spring are connected between the outer wall of the telescopic parent rod and the tail end of the fixing pin.

[0008] As preferred, the limiting mechanism comprises a limiting column, the top end of the limiting column is clamped in a limiting groove, one side of the support frame is fixedly provided with a lug, a pull rod is embedded on the lug, the top end of the pull rod is fixedly connected with the limiting column, a tension spring is embedded on the limiting column, and the two ends of the tension spring are connected between the lower side of the lug and the tail end of the pull rod.

[0009] As preferred, the rotating mechanism comprises a driving box fixedly arranged on one side of the support frame, the driving box is provided with a worm gear and a worm, and the worm gear and the worm are engaged, one end of the threaded rod is embedded in the support frame and fixedly connected with the worm gear, a rotating block is arranged above the driving box, and the upper end of the worm is embedded in the driving box and fixedly connected with the lower side of the rotating block.

[0010] As preferred, the inner side of each of the clamping blocks is fixedly provided with an anti-skid pad, the upper side of each of the two support frames is provided with a through groove, and the lower end of each of the two support frames is provided with a universal wheel.

[0011] The beneficial effects of the present application are as follows:

[0012] 1. The rotating mechanism can drive the threaded rod to rotate, so that the threaded cylinder moves along the threaded rod, thereby adjusting the distance between the two support frames, and finally adjusting the distance between the clamping blocks, thereby fixing and installing column chromatography of different sizes, thereby increasing the use range of the device, facilitating popularization and use, and adjusting the overall length of the telescopic frame to adjust the distance between the two clamping blocks, thereby facilitating the clamping blocks to fix column chromatography of different heights, further increasing the use range of the device, and adjusting the clamping blocks to the appropriate position to increase the fixing stability of the column chromatography body.

[0013] 2, when the column chromatography is fixed, the column chromatography can be rotated, so as to adjust the angle of the column chromatography body, at this time, the short shaft can be driven to rotate, so as to drive the rotating wheel to rotate, then the rotating wheel can be limited to rotate through the limiting mechanism, so as to fix the angle of the column chromatography body, therefore, the inclination angle of the chromatography column can be adjusted to test the flow speed and separation effect of the sample in the filler column, and the residence time and separation degree of the sample in the filler column can be controlled by adjusting the angle of the column chromatography, so as to obtain the best separation effect. At the same time, the angle of the chromatography column can also affect the uniformity of the liquid phase in the filler column, therefore, the liquid phase can be uniformly distributed in the filler column by adjusting the angle, so as to avoid the unevenness of the liquid phase flow in the column, thereby improving the stability and repeatability of the separation. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 Structure schematic view of the embodiment;

[0015] Figure 2 Structure schematic view of the telescopic frame in the embodiment;

[0016] Figure 3 Structure schematic view of one side of the support frame in the embodiment;

[0017] Figure 4 Partial structure top view of the embodiment;

[0018] Figure 5 Partial structure schematic view of the embodiment.

[0019] Fig. 1 is a support frame; Fig. 2 is a column chromatography body; Fig. 3 is a short shaft; Fig. 4 is a telescopic frame; Fig. 5 is a connecting shaft; Fig. 6 is a clamping block; Fig. 7 is a rotating wheel; Fig. 8 is a limiting groove; Fig. 9 is a limiting mechanism; Fig. 10 is a threaded cylinder; Fig. 11 is a threaded rod; Fig. 12 is a rotating mechanism; Fig. 13 is a guide cylinder; Fig. 14 is a guide column; Fig. 15 is a telescopic sub-rod; Fig. 16 is a telescopic main rod; Fig. 17 is a fixing pin; Fig. 18 is a fixing hole; Fig. 19 is a return spring; Fig. 20 is a limiting column; Fig. 21 is a protruding block; Fig. 22 is a pull rod; Fig. 23 is a tension spring; Fig. 24 is a driving box; Fig. 25 is a worm wheel; Fig. 26 is a worm; Fig. 27 is a rotating block; Fig. 28 is an anti-skid pad; Fig. 29 is a through groove; and Fig. 30 is a universal wheel. DETAILED DESCRIPTION

[0020] The following description is only a preferred embodiment of the present application, and the protection scope is not limited to the embodiment only, and any technical solution falling within the idea of the present application shall fall within the protection scope of the present application. Identical parts are denoted by identical reference numerals. It should be noted that the words "front", "back", "left", "right", "up" and "down" used in the following description refer to the directions in the drawings, and the words "bottom" and "top", "inner" and "outer" refer to the directions towards or away from the geometric center of a particular part.

[0021] As Figures 1 to 5 A new type of column chromatography device, as shown in the figure, including two support frame 1 and column chromatography body 2, the inner side of two support frame 1 is respectively connected with short shaft 3, one end of two short shaft 3 is respectively fixed with telescopic frame 4, one side of each telescopic frame 4 is respectively connected with two connecting shaft 5, the other end of two connecting shaft 5 is respectively fixed with clamping block 6, and the column chromatography body 2 is clamped between several clamping blocks 6, and the column chromatography body 2 can be installed and fixed through several clamping blocks 6. One side of one of the support frame 1 is fixed with a threaded cylinder 10, the other side of the other support frame 1 is rotatably connected with a threaded rod 11, one end of the threaded rod 11 is provided in the threaded cylinder 10, one side of the support frame 1 is provided with a rotating mechanism 12, and the rotating mechanism 12 is connected with the threaded rod 11, the rotating mechanism 12 can drive the threaded rod 11 to rotate, so that the threaded cylinder 10 can move along the threaded rod 11, thus the distance between the two support frames 1 can be adjusted, and finally the distance between the several clamping blocks 6 can be adjusted, so that the column chromatography of different sizes can be fixed and installed, thus the use range of the device can be increased, so as to facilitate the promotion and use. One side of one of the support frame 1 is fixed with two guide cylinders 13, the other side of the other support frame 1 is fixed with two guide columns 14, one end of the two guide columns 14 is respectively provided in the guide cylinder 13, when the distance between the two support frames 1 is adjusted, the two guide columns 14 can slide along the guide cylinder 13 respectively, so that the moving track of the two support frames 1 is more straight and stable, and the clamping block 6 is more stable when clamping the column chromatography.

[0022] The rotating mechanism 12 includes a drive box 24 fixed on one side of the support frame 1, a worm gear 25 and a worm 26 are arranged in the drive box 24, and the worm gear 25 and the worm 26 are engaged, one end of the threaded rod 11 is provided in the support frame 1 and is fixedly connected with the worm gear 25, a rotating block 27 is arranged above the drive box 24, the upper end of the worm 26 is provided in the drive box 24 and is fixedly connected with the lower side of the rotating block 27, the rotating block 27 can drive the worm 26 to rotate by rotating, thus driving the worm gear 25 to rotate, finally driving the threaded rod 11 to rotate, and because the worm gear 25 and the worm 26 have self-locking property, after the clamping block 6 is fixed on the surface of the column chromatography body 2, the reverse rotation can be prevented to cause the clamping block 6 to loosen, further ensuring the use stability of the column chromatography.

[0023] The inner side of the several clamping blocks 6 is fixed with an anti-skid pad 28, the fixed column chromatography can be prevented from sliding and falling off through the anti-skid pad 28, the upper side of the two support frames 1 is respectively provided with a through slot 29, the lower end of the two support frames 1 is respectively provided with a universal wheel 30, the two support frames 1 can be lifted through the through slot 29, so that the position of the column chromatography can be moved, and the movement and fixation of the support frame 1 can also be realized through the several universal wheels 30, so that the position of the column chromatography can be moved and adjusted.

[0024] The telescopic frame 4 is provided with a telescopic child rod 15 and a telescopic parent rod 16, the lower end of the telescopic child rod 15 is slidably embedded in the telescopic parent rod 16, one side of the telescopic parent rod 16 is provided with a fixing pin 17, the telescopic child rod 15 is provided with a plurality of fixing holes 18, the top end of the fixing pin 17 is embedded in the fixing hole 18, a reset spring 19 is embedded on the fixing pin 17, and the two ends of the reset spring 19 are respectively connected between the outer wall of the telescopic parent rod 16 and the tail end of the fixing pin 17. The overall length of the telescopic frame 4 can be adjusted to adjust the distance between the two clamping blocks 6, so that the clamping block 6 can be fixed to the column chromatography of different heights, which can further increase the use range of the device, and the clamping block 6 can be adjusted to the appropriate position to increase the fixing stability of the column chromatography body 2. At this time, the user only needs to pull the fixing pin 17 to make the fixing pin 17 come out of the fixing hole 18, so that the reset spring 19 is stretched to generate elastic force, and then the telescopic child rod 15 can be moved up and down to adjust the overall length of the telescopic frame 4. When the telescopic child rod 15 is pulled to the appropriate position, the reset spring 19 can drive the fixing pin 17 to return to the initial position, so that the fixing pin 17 can be inserted into the corresponding fixing hole 18 again, thereby realizing the fixation between the telescopic child rod 15 and the telescopic parent rod 16.

[0025] One side of the support frame 1 is provided with a rotating wheel 7, one end of the short shaft 3 is embedded in the support frame 1 and is fixedly connected with one side of the rotating wheel 7, the rotating wheel 7 is provided with a plurality of limiting grooves 8, one side of the support frame 1 is provided with a limiting mechanism 9, and the limiting mechanism 9 is matched with the limiting grooves 8. When the column chromatography is fixed, the column chromatography can be rotated to adjust the angle of the column chromatography body 2. At this time, the short shaft 3 can be rotated to drive the rotating wheel 7 to rotate, and then the limiting mechanism 9 can be used to limit the rotation of the rotating wheel 7 to fix the angle of the column chromatography body 2. Therefore, the inclination angle of the chromatography column can be adjusted to test the flow speed and separation effect of the sample in the filler column, and the residence time and separation degree of the sample in the filler column can be controlled by adjusting the angle of the column chromatography to obtain the best separation effect. At the same time, the angle of the chromatography column can also affect the uniformity of the liquid phase in the filler column, so that the liquid phase can be uniformly distributed in the filler column by adjusting the angle to avoid the uneven flow of the liquid phase in the column, thereby improving the stability and repeatability of the separation.

[0026] The limiting mechanism 9 comprises a limiting column 20, the top end of the limiting column 20 is clamped in the limiting groove 8, one side of the support frame 1 is fixedly provided with a lug 21, the lug 21 is provided with a pull rod 22 penetratingly, the top end of the pull rod 22 is fixedly connected with the limiting column 20, the limiting column 20 is provided with a tension spring 23 penetratingly, the two ends of the tension spring 23 are respectively connected between the lower side of the lug 21 and the tail end of the pull rod 22, by pulling the pull rod 22, the limiting column 20 can be driven to be out of the limiting groove 8, at this time, the tension spring 23 is stretched to generate elastic force, then the rotating of the rotating wheel 7 can be realized, so that the angle of the column chromatography can be adjusted, when the angle of the column chromatography is adjusted, the tension spring 23 can return to the initial position, so as to drive the limiting column 20 to be top into the corresponding limiting groove 8 again, so as to limit the rotating of the rotating wheel 7, finally the inclination angle of the column chromatography can be fixed.

[0027] The above-described specific embodiments further specifically describe the technical problems solved by the present application, technical solutions and beneficial effects. It should be understood that the above-described specific embodiments are merely specific embodiments of the present application and are not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A novel column chromatography device, characterized by, The utility model provides a kind of column chromatography device, including two support frames (1) and column chromatography body (2), the inner side of two support frames (1) is rotatably connected with short shaft (3) respectively, the one end of two short shafts (3) is fixed with telescopic frame (4) respectively, the one side of each telescopic frame (4) is connected with two connecting shafts (5) respectively, the other end of two connecting shafts (5) is fixed with clamping block (6) respectively, and column chromatography body (2) is clamped between several clamping blocks (6), the one side of support frame (1) is provided with rotating wheel (7), the one end of short shaft (3) is arranged in support frame (1), and is fixedly connected with the one side of rotating wheel (7), rotating wheel (7) is provided with several limit grooves (8), the one side of support frame (1) is provided with limiting mechanism (9), and limiting mechanism (9) is matched with limit groove (8), the lower side of one support frame (1) is fixed with threaded cylinder (10), the one side of another support frame (1) is rotatably connected with threaded rod (11), and the one end of threaded rod (11) is arranged in threaded cylinder (10), the one side of support frame (1) is provided with rotating mechanism (12), and rotating mechanism (12) is connected with threaded rod (11), the lower side of one support frame (1) is fixed with two guide cylinders (13), the lower side of another support frame (1) is fixed with two guide columns (14), the one end of two guide columns (14) is arranged in guide cylinder (13) respectively.

2. A novel column chromatography device according to claim 1, characterized in that, The telescopic frame (4) is telescopic sub-rod (15) and telescopic female rod (16), and the lower end of telescopic sub-rod (15) is slidably embedded in telescopic female rod (16), the one side of telescopic female rod (16) is provided with fixed pin (17), telescopic sub-rod (15) is provided with several fixed holes (18), and the top end of fixed pin (17) is arranged in fixed hole (18), fixed pin (17) is provided with reset spring (19), and the two ends of reset spring (19) are connected between the outer wall of telescopic female rod (16) and the tail end of fixed pin (17).

3. A novel column chromatography device according to claim 2, characterized in that, The limiting mechanism (9) includes limiting column (20), and the top end of limiting column (20) is clamped in limit groove (8), the one side of support frame (1) is fixed with protruding block (21), the one side of protruding block (21) is provided with pull rod (22), and the top end of pull rod (22) is fixedly connected with limiting column (20), limiting column (20) is provided with tension spring (23), and the two ends of tension spring (23) are connected between the lower side of protruding block (21) and the tail end of pull rod (22).

4. A novel column chromatography device according to claim 3, characterized in that, The rotating mechanism (12) comprises a driving box (24) fixed on one side of the support frame (1), a worm wheel (25) and a worm (26) are arranged in the driving box (24), the worm wheel (25) and the worm (26) are engaged, one end of the threaded rod (11) penetrates the support frame (1) and is fixedly connected with the worm wheel (25), and a rotating block (27) is arranged above the driving box (24), the upper end of the worm (26) penetrates the driving box (24) and is fixedly connected with the lower side of the rotating block (27).

5. A novel column chromatography device according to claim 4, characterized in that, The inner side of each of the plurality of clamping blocks (6) is fixedly provided with an anti-skid pad (28), the upper side of each of the two support frames (1) is provided with a through groove (29), and the lower end of each of the two support frames (1) is provided with a universal wheel (30).

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