Reaction container, reaction container assembly and polypeptide synthesis equipment
By setting an opening and a feed pipe at the bottom of the reaction vessel, the problems of liquid splashing and space occupation are solved, enabling a highly efficient peptide synthesis reaction and improving the space utilization and user experience of the reaction vessel.
Patent Information
- Application Number
- CN202423032786.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-12-26
- Estimated Expiration
- 2034-12-09
AI Technical Summary
The top opening of a traditional reaction vessel causes piperidine solution to splash and contaminate the vessel wall, and the nitrogen pipeline occupies the reaction space, affecting the efficiency of peptide synthesis.
Design a reaction vessel with an opening at the bottom and a feed pipe extending from a support. The feed port includes a liquid inlet and a gas inlet, allowing liquid and gas to enter the reaction vessel from the bottom, avoiding splashing and saving reaction space.
It effectively prevents liquid and gas splash contamination, improves reaction space utilization, enhances peptide synthesis efficiency, and improves user experience.
Smart Images
Figure CN223717093U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of polypeptide synthesis technology, specifically, a reaction container, a reaction container assembly and a polypeptide synthesis equipment with same. BACKGROUND
[0002] As a key field of biological medicine innovation and research and development, polypeptide synthesis technology has broad development prospects. With the advantages of high biological activity, high specificity, low side effects and difficulty in accumulation in the body, polypeptide drugs have become a global hotspot for new drug research and development. At present, more than 80 polypeptide drugs have been approved for marketing worldwide, with a market size of 62.8 billion US dollars in 2020, and it is expected to grow to 96 billion US dollars in 2025, showing strong growth momentum.
[0003] The traditional reaction cup has resin placed inside. The resin can be used as a solid carrier, which is usually pre-activated and has a protective group such as Fmoc (fluorenylmethyloxycarbonyl). The top of the reaction cup is provided with an opening. A 20% piperidine solution can be added to the reaction cup through the opening, so that the resin can be deprotected, and then the N-terminal Fmoc protective group can be removed. Then, a gas such as nitrogen can be added to the reaction cup through the opening, so that bubbling (condensation reaction) can be carried out. Then, subsequent processes can be carried out in the reaction cup, and finally all amino acids are connected to the resin in a predetermined order to form a complete polypeptide chain.
[0004] Since the opening of the reaction cup is located at the top, the piperidine solution will splash onto the cup wall during the addition process, and the piperidine solution that slides down the cup wall during the subsequent process will contaminate the subsequent process. In order to improve the effect of bubbling, the pipe for adding nitrogen needs to be inserted into the bottom of the reaction cup, which will occupy the space in the reaction cup, resulting in a smaller reaction space. SUMMARY
[0005] In order to at least partially solve the problems existing in the prior art, according to one aspect of the utility model, a reaction container is provided. The reaction container comprises: a reaction cup, the bottom of the reaction cup is provided with a bottom opening; a supporting member, the reaction cup is supported on the supporting member; and a feed pipe, the feed pipe extends downward from the supporting member, and the side of the feed pipe is provided with a feed port communicating with the bottom opening.
[0006] Exemplarily, the feed port comprises a liquid inlet and a gas inlet.
[0007] Exemplarily, the liquid inlet and the gas inlet are oppositely arranged along a lateral direction perpendicular to the extension direction of the feed pipe.
[0008] Exemplarily, the liquid inlet and the gas inlet are completely staggered along the extension direction of the feed pipe.
[0009] Exemplarily, the supporting member comprises a cup holder and a sealing ring, the reaction cup comprises a large-size section at an upper end, a small-size section at a lower end, and a transition section connected between the upper end and the lower end, the transition section is supported on the cup holder, the small-size section is inserted into the cup holder, the sealing ring surrounds the small-size section and is clamped between the cup holder and the small-size section, and the bottom opening is arranged at the bottom of the small-size section.
[0010] Exemplarily, the feeding port is provided with a one-way valve.
[0011] Exemplarily, the reaction cup comprises a large-size section at an upper end, a small-size section at a lower end, and a transition section connected between the upper end and the lower end, and the reaction cup is provided with a filter screen supported on the transition section.
[0012] According to another aspect of the present application, a reaction container assembly is also provided. The reaction container assembly comprises a plurality of any of the reaction containers described above.
[0013] Exemplarily, the reaction container assembly has a plurality of groups, each group of the plurality of groups comprises at least one reaction container, and at least two groups of the plurality of groups are arranged staggered in a vertical direction.
[0014] According to still another aspect of the present application, a polypeptide synthesis device is also provided. The polypeptide synthesis device comprises any of the reaction containers described above, and the feeding port comprises a liquid inlet for adding a piperidine solution and a gas inlet for introducing nitrogen gas.
[0015] In actual application, the liquid and / or gas and the like can enter the feeding pipe through the feeding port, and then can enter the reaction cup through the bottom opening. The liquid includes but is not limited to a piperidine solution. The gas includes but is not limited to nitrogen. In this way, the liquid and / or gas and the like will rise from the bottom of the reaction cup in the process of entering, so as not to splash on the cup wall of the reaction cup, and thus will not cause pollution to the subsequent process. Moreover, the reaction cup does not need to be provided with a pipeline for guiding the liquid and / or gas to the bottom, and the space in the reaction cup can be used for reaction in whole, and the utilization rate of the space in the reaction cup is high.
[0016] A series of simplified forms are introduced in the utility model content, which will be further described in detail in the specific embodiment part. The utility model content part does not mean trying to limit the key features and necessary technical features of the claimed technical solution, and more does not mean trying to determine the protection scope of the claimed technical solution.
[0017] The advantages and features of the present application will be described in detail below with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS
[0018] The following drawings for the utility model are hereby incorporated as part of the utility model for the purpose of understanding the utility model. The drawings shown in the utility model embodiments and their descriptions are used to explain the principles of the utility model. In the drawings,
[0019] Figure 1 is a perspective view of a reaction vessel assembly according to an exemplary embodiment of the utility model;
[0020] Figure 2 is Figure 1 is a front view of a reaction vessel shown in the drawings;
[0021] Figure 3 is Figure 2 is a sectional view of a reaction vessel shown in the drawings;
[0022] Figure 4 is Figure 2 is a front view of a reaction cup shown in the drawings; and
[0023] Figure 5 is Figure 2 is a sectional view of a reaction cup shown in the drawings.
[0024] Among them, the above drawings include the following reference signs:
[0025] 100, reaction vessel; 200, reaction cup; 201, bottom opening; 202, top opening; 210, large size section; 220, small size section; 230, transition section; 300, support; 310, cup holder; 320, sealing ring; 400, feed pipe; 410, feed port; 411, liquid inlet; 412, gas inlet; 420, feed channel; 500, filter screen; 610, first group; 620, second group. DETAILED DESCRIPTION
[0026] In the following description, a large number of details are provided so as to be able to thoroughly understand the utility model. However, the person skilled in the art can understand that the following description only exemplarily shows the preferred embodiments of the utility model, and the utility model can be implemented without one or more such details. In addition, in order to avoid confusion with the utility model, some technical features known in the art are not described in detail.
[0027] According to one aspect of the present application, a reaction vessel is provided. The reaction vessel can be used for performing a reaction such as a polypeptide synthesis reaction or any other suitable reaction. According to another aspect of the present application, a reaction vessel assembly is also provided. The reaction vessel assembly can include a plurality of reaction vessels. The reaction vessel can be applied to any suitable device including but not limited to a polypeptide synthesis device. Therefore, according to another aspect of the present application, a polypeptide synthesis device is also provided. The reaction vessel, the reaction vessel assembly and the polypeptide synthesis device according to the embodiments of the present application will be described in detail below with reference to the accompanying drawings.
[0028] As shown in Figures 2-5 , the reaction vessel 100 can include a reaction cup 200, a support member 300 and a feed pipe 400.
[0029] The bottom of the reaction cup 200 can be provided with a bottom opening 201. Liquid and / or gas and other materials can enter the reaction cup 200 through the bottom opening 201. The reaction cup 200 can be used to perform a reaction such as a polypeptide synthesis reaction or any other suitable reaction. Exemplarily, the reaction cup 200 can be made of glass or other transparent materials. In this way, the relevant personnel can conveniently observe the reaction inside the reaction cup 200 from outside the reaction cup 200. Exemplarily, the top of the reaction cup 200 can be provided with a top opening 202. Resin and other materials can enter the reaction cup 200 through the top opening 202. Of course, resin and other materials can also be pre-entered into the reaction cup 200 through the bottom opening 201 or pre-placed in the reaction cup 200 during the manufacturing process of the reaction cup 200. The top opening 202 can also serve as a backup opening to provide users with more choices.
[0030] The support member 300 can serve as the base of the reaction vessel 100. When the reaction vessel 100 is applied to other devices, the support member 300 can be used to fix to the components outside the reaction vessel 100. The reaction cup 200 can be supported on the support member 300.
[0031] The feed pipe 400 can extend downward from the support member 300. The feed pipe 400 can be connected to the bottom of the support member 300 by welding, bonding or one-piece forming and any other suitable means. The side of the feed pipe 400 can be provided with a feed port 410. The feed port 410 can be directly or indirectly communicated to the bottom opening 201. In some embodiments, the feed pipe 400 can be provided with a feed passage 420 extending in the vertical direction. The top end of the feed passage 420 can be aligned and communicated with the bottom opening 201. The lower end of the feed passage 420 can be communicated with the feed port 410. In this way, the feed passage 420 can be communicated between the feed port 410 and the bottom opening 201. The feed port 410 can be used to connect with a device for supplying materials. The device for supplying materials includes but is not limited to a liquid storage device and / or a gas storage device.
[0032] In actual application, the liquid and / or gas and the like can enter the feeding pipe 400 through the feeding port 410, and then can enter the reaction cup 200 through the bottom opening 201. The liquid includes but is not limited to piperidine solution. The gas includes but is not limited to nitrogen. In this way, the liquid and / or gas and the like can rise from the bottom of the reaction cup 200 in the process of entering, so as not to splash on the cup wall of the reaction cup 200, and thus not to cause pollution to the subsequent process. In addition, the reaction cup 200 does not need to be provided with a pipeline for guiding the liquid and / or gas to the bottom, and the space in the reaction cup 200 can be used for reaction entirely, and the utilization rate of the space in the reaction cup 200 is high.
[0033] Exemplarily, as shown in Figure 2-3 The feeding port 410 can include a liquid inlet 411 and a gas inlet 412. The liquid can enter the feeding pipe 400 through the liquid inlet 411, and then can enter the reaction cup 200 through the bottom opening 201. The gas can enter the feeding pipe 400 through the gas inlet 412, and then can enter the reaction cup 200 through the bottom opening 201. In the embodiment in which the feeding pipe 400 is provided with the feeding channel 420 extending in the vertical direction, the lower ends of the feeding channel 420 can be in communication with the liquid inlet 411 and the gas inlet 412, respectively. The liquid inlet 411 can be used to be connected to a liquid storage device. The gas inlet 412 can be used to be connected to a gas storage device. By providing the liquid inlet 411 and the gas inlet 412, the feeding pipe 400 can have a basis connected to the liquid storage device and the gas storage device, respectively, so that it is not necessary to switch the connection between the liquid storage device and the gas storage device, and the user experience is good. In addition, if necessary, the liquid and the gas can enter the reaction cup 200 through the bottom opening 201 at the same time.
[0034] Exemplarily, as shown in Figure 2-3 In the lateral direction of the feeding pipe 400, the liquid inlet 411 and the gas inlet 412 can be oppositely arranged. The lateral direction can be perpendicular to the extension direction of the feeding pipe 400. Generally, the extension direction of the feeding pipe 400 can be the vertical direction. Therefore, the lateral direction can be the horizontal direction. In this way, the distance between the liquid inlet 411 and the gas inlet 412 is far, so that there is enough space for connecting to the liquid storage device and the gas storage device. Exemplarily, the liquid inlet 411 can be arranged at the back side of the feeding pipe 400. The gas inlet 412 can be arranged at the front side of the feeding pipe 400. Here and hereinafter, the orientation term "back" generally refers to the side far away from the relevant person. Therefore, here and hereinafter, the orientation term "front" generally refers to the side close to the relevant person.
[0035] Exemplarily, as shown in Figure 2-3As shown, the liquid inlet 411 and the gas inlet 412 can be completely staggered along the extension direction of the feed pipe 400. In this way, the liquid inlet 411 and the gas inlet 412 are not directly opposite. In this way, during the process that the liquid enters the feed pipe 400 through the liquid inlet 411, the liquid will not directly hit the gas inlet 412, so as to avoid the liquid flowing out through the gas inlet 412. Similarly, during the process that the gas enters the feed pipe 400 through the gas inlet 412, the gas will not directly hit the liquid inlet 411, so as to avoid the liquid flowing out through the liquid inlet 411.
[0036] Exemplarily, as Figure 2-3As shown, the holder 300 can include a cup holder 310 and a seal ring 320. The cup holder 310 can be annular. The cup holder 310 can be used to secure to a component outside of the reaction vessel 100 when the reaction vessel 100 is applied to other devices. The feed tube 400 can extend downward from the cup holder 310. The feed tube 400 can be connected to the bottom of the cup holder 310 by any suitable means, including but not limited to welding, adhesion, or one-piece molding. The reaction cup 200 can include a large-size section 210, a small-size section 220, and a transition section 230. The large-size section 210 can be located at the upper end of the reaction cup 200. The small-size section 220 can be located at the lower end of the reaction cup 200. The transition section 230 can be connected between the upper end and the lower end of the reaction cup 200. That is, in the vertical direction, the large-size section 210, the transition section 230, and the small-size section 220 can be connected in sequence. In some embodiments, the large-size section 210 and the transition section 230 can be smoothly transitioned. The small-size section 220 and the transition section 230 can be smoothly transitioned. The bottom opening 201 can be provided at the bottom of the small-size section 220. The top opening 202 can be provided at the top of the large-size section 210. The small-size section 220 can be inserted into the cup holder 310. The transition section 230 can be supported on the cup holder 310. The seal ring 320 can surround the small-size section 220. Also, the seal ring 320 can be clamped between the cup holder 310 and the small-size section 220. In actual applications, the seal ring 320 can be pre-placed in the cup holder 310. When the reaction cup 200 needs to be installed, the small-size section 220 can be inserted into the seal ring 320 in the cup holder 310, so that the seal ring 320 can be clamped between the cup holder 310 and the small-size section 220. With the insertion, when the transition section 230 is supported on the cup holder 310, the reaction cup 200 is installed in place. In this way, the reaction cup 200 can be easily installed and removed. Also, the seal ring 320 can provide a large friction force to the small-size section 220, so that the reaction cup 200 can be prevented from being easily displaced, and the reaction cup 200 can be ensured to be in the expected position. In addition, the seal ring 320 can also seal the gap between the small-size section 220 and the cup holder 310, so as to prevent the liquid and / or gas passing through the feed tube 400 from leaking through the gap due to excessive pressure. The seal ring 320 can be of any type known in the art or that can exist in the future, including but not limited to a generic seal. The material of the seal ring 320 can be any, including but not limited to polytetrafluoroethylene (PTFE).
[0037] Exemplarily, as Figure 2-3As shown, a filter screen 500 can be provided in the reaction cup 200. The filter screen 500 can be supported on the transition section 230 by welding or adhesion or any suitable means. The filter screen 500 can be used to support a resin or the like. The liquid and / or gas passing through the feed pipe 400 can pass through the filter screen 500, and thus can react with the resin or the like supported on the filter screen 500. Exemplarily, the filter screen 500 can be a sand core with a mesh size of about 40 mesh, and thus can be used to support resin particles with a mesh size of about 100-200 mesh.
[0038] Exemplarily, a one-way valve can be provided in the feed port 410. The one-way valve can allow the liquid and / or gas to pass from outside the feed port 410 into the feed pipe 400 in one direction. In this way, the liquid and / or gas cannot flow out of the feed port 410. In the embodiment in which the feed port 410 includes the liquid inlet 411 and the gas inlet 412, a one-way valve can be provided in each of the liquid inlet 411 and the gas inlet 412. The one-way valves provided in the liquid inlet 411 and the gas inlet 412 can be the same or different.
[0039] In the embodiment in which the reaction vessel 100 is applied to a reaction vessel assembly, as shown in Figure 1 The reaction vessel assembly can include a plurality of reaction vessels 100. The plurality of reaction vessels 100 can be arranged in any suitable manner. Each reaction vessel 100 can perform a reaction individually. In this way, the reaction vessel assembly can improve the throughput of the reaction and improve the user experience.
[0040] Exemplarily, as shown in Figure 1 The reaction vessel assembly can have a plurality of groups. Each group of the plurality of groups can include at least one reaction vessel 100, for example, two, three or more. In the embodiment shown in the figure, each group can include ten reaction vessels 100. The reaction vessels 100 of each group can be arranged in a straight line. At least two groups of the plurality of groups can be arranged in a vertical direction. The reaction vessels 100 arranged in the vertical direction can facilitate the routing of the pipeline connecting the feed port 410. Moreover, it is convenient for relevant personnel to observe the reaction cups 200 at different heights. In the embodiment shown in the figure, the reaction vessel assembly can have a first group 610 and a second group 620. The first group 610 and the second group 620 can each include ten reaction vessels 100. The first group 610 and the second group 620 can be arranged in a vertical direction. Exemplarily, the reaction vessel assembly can also have other groups. The other groups can also be arranged in a vertical direction with the first group 610 and the second group 620, or arranged in a horizontal direction parallel to the first group 610, or arranged in a horizontal direction parallel to the second group 620.
[0041] In embodiments where the reaction vessel 100 is applied to a polypeptide synthesis apparatus, the feed port 410 can include a liquid inlet 411 and a gas inlet 412. The liquid inlet 411 can be used to add a piperidine solution. The gas inlet 412 can be used to introduce nitrogen gas. The piperidine solution can deprotect the resin within the reaction cup 200. The nitrogen gas can allow the resin within the reaction cup 200 to be in sufficient contact with the piperidine solution, which can speed up the reaction.
[0042] In the description of the present application, it should be understood that the orientation words such as "front", "back", "upper", "lower", "left", "right", "transverse", "vertical", "perpendicular", "horizontal", and "top", "bottom" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and in the absence of contrary description, these orientation words do not indicate and imply that the devices or elements referred to must have a specific orientation or be constructed and operated in a specific orientation, therefore cannot be understood as a limitation on the protection scope of the present application; the orientation words "inner" and "outer" refer to the inner and outer of the contour of each component itself.
[0043] For the convenience of description, regional relative terms such as "on", "above", "upper surface", "upper" and the like can be used here to describe the regional positional relationship of one or more components or features shown in the drawings with other components or features. It should be understood that the regional relative terms not only include the orientation of the components described in the drawings, but also include different orientations in use or operation. For example, if the components in the drawings are inverted as a whole, the components "above" or "on" other components or features will include the case of "below" or "under" other components or structures. Thus, the exemplary term "above" can include both "above" and "below" orientations. In addition, these components or features can also be positioned at other different angles (for example, rotated by 90 degrees or other angles), and all these cases are intended to be included herein.
[0044] It should be noted that the terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms "comprise" and / or "include" are used in the specification, they indicate the presence of a feature, step, operation, component, assembly and / or combination thereof.
[0045] It should be noted that the terms "first", "second", and the like in the description and in the claims of the present application and in the above-described drawings are used only for distinguishing between similar objects and do not necessarily have to describe a specific sequential or chronological order. It is to be understood that the data so distinguished can be interchanged, under appropriate circumstances, such that the embodiments of the present application described herein can be practiced in other than the illustrated or described order.
[0046] The utility model has carried on the explanation through the above embodiment, but should understand, the above embodiment is only for example and the purpose of explanation, and not intend to limit the utility model to the range of described embodiment. In addition, the person skilled in the art can understand that the utility model is not limited to the above embodiment, and more kinds of variations and modifications can be made according to the teaching of the utility model, and these variations and modifications all fall within the scope of the utility model claimed. The protection scope of the utility model is defined by the attached claims and its equivalent scope.
Claims
1. A reaction vessel, characterized in that, include: A reaction cup, wherein the bottom of the reaction cup is provided with a bottom opening; Support, on which the reaction cup is supported; as well as The feed pipe extends downward from the support member, and a feed port connected to the bottom opening is provided on the side of the feed pipe.
2. The reaction vessel as described in claim 1, characterized in that, The feed inlet includes a liquid inlet and an air inlet.
3. The reaction vessel as described in claim 2, characterized in that, The liquid inlet and the air inlet are arranged opposite each other in a lateral direction perpendicular to the extension direction of the feed pipe.
4. The reaction vessel as described in claim 2, characterized in that, Along the extension direction of the feed pipe, the liquid inlet and the air inlet are completely offset.
5. The reaction vessel as described in claim 1, characterized in that, The support includes a cup holder and a sealing ring. The reaction cup includes a large upper section, a small lower section, and a transition section connecting the upper and lower ends. The transition section is supported on the cup holder. The small lower section is inserted into the cup holder. The sealing ring surrounds the small lower section and is clamped between the cup holder and the small lower section. The bottom opening is located at the bottom of the small lower section.
6. The reaction vessel as described in claim 1, characterized in that, A one-way valve is installed inside the feed inlet.
7. The reaction vessel as claimed in claim 1, characterized in that, The reaction cup includes a large-sized section at the upper end, a small-sized section at the lower end, and a transition section connecting the upper end and the lower end. A filter screen supported on the transition section is provided inside the reaction cup.
8. A reaction vessel assembly, characterized in that, The reaction vessel assembly includes a plurality of reaction vessels as described in any one of claims 1-7.
9. The reaction vessel assembly as claimed in claim 8, characterized in that, The reaction vessel assembly has multiple sets, each set including at least one of the reaction vessels, and at least two sets of the multiple sets are staggered in the vertical direction.
10. A polypeptide synthesis apparatus, characterized in that, The reaction vessel includes any one of claims 1-7, wherein the feed port includes a liquid inlet for adding piperidine solution and a nitrogen inlet.