Reagent extraction mechanism and gene sequencing system

Abstract

The utility model discloses a reagent extraction mechanism and a gene sequencing system. Wherein the reagent extraction mechanism comprises a bearing frame on which a first type of reagent needle assembly and a second type of reagent needle assembly, which are arranged in a step shape, are mounted; a first type kit assembly and a second type kit assembly are correspondingly arranged below the first type reagent needle assembly and the second type reagent needle assembly; the first type of kit assembly is mounted at the top of the second type of kit assembly; the power device is installed on the bearing frame, a bearing supporting plate is installed at the power output end of the power device, and the second type kit assembly is fixedly connected with the bearing supporting plate; and the bearing support plate has a degree of freedom for driving the second type of kit assembly and the first type of kit assembly to lift under the driving of the power device. According to the device, membrane rupture and reagent extraction are realized through the immobile reagent needle assembly and the rising movement of the kit assembly, so that the length of a pipeline is effectively reduced, the fluid pressure is reduced, the reagent is saved, and smooth, efficient and accurate reagent extraction is realized.

Description

Technical Field

[0001] This disclosure relates to the field of biomedical testing, and in particular to a reagent extraction mechanism and a gene sequencing system. Background Technology

[0002] Reagents used in gene sequencing are generally stored at around 4°C. When using them, reagents need to be drawn from the kit. The kit opening is usually sealed with a membrane, and a reagent needle needs to break through the membrane to enter the kit and draw the reagents. The current method is that the kit is stationary, and the reagent needle moves to puncture the membrane and enter the kit to draw the reagents. Because the reagent draw requirements of all locations of the kit need to be met, the transfer tubing connected to the reagent needle is designed to be very long, which requires a large fluid pressure to draw the target amount of reagent. At the same time, the long tubing also leads to reagent waste due to residue in the tubing wall. Utility Model Content

[0003] In view of this, the present disclosure provides a reagent extraction mechanism and a gene sequencing system, which at least partially solve the problems of excessive reagent waste and high fluid extraction pressure in the prior art.

[0004] In a first aspect, embodiments of this disclosure provide a reagent extraction mechanism, specifically including:

[0005] A support frame on which a first type of reagent needle assembly and a second type of reagent needle assembly are mounted in a stepped arrangement; a first type of reagent kit assembly and a second type of reagent kit assembly are correspondingly arranged below the first type of reagent needle assembly and the second type of reagent needle assembly;

[0006] The first type of reagent kit assembly is mounted on top of the second type of reagent kit assembly, and the first type of reagent kit in the first type of reagent kit assembly has the degree of freedom to move along the longitudinal axis of the second type of reagent kit assembly;

[0007] A power unit installed on the support frame, a support plate is installed on the power output end of the power unit, and the second type of reagent kit assembly is fixedly connected to the support plate;

[0008] The support tray, driven by the power device, has the freedom to move the second type of reagent kit component and the first type of reagent kit component up and down.

[0009] Optionally, the power unit includes a drive motor and a lead screw mounted on the power output end of the drive motor;

[0010] The support frame includes a base plate, and a first side plate and a second side plate are provided on the base plate, with the second side plate being disposed opposite to the first side plate;

[0011] The first side plate is provided with a motor mounting part and a linear guide rail that match the drive motor;

[0012] The support tray includes a first support block, a second support block, and a support plate. The second type of reagent kit component is disposed on the top of the support plate, and the first support block and the second support block are respectively fixedly disposed on both sides of the support plate.

[0013] The first bearing block is engaged with the linear guide rail, and the first bearing block is matched with the lead screw; the lead screw rotates under the drive of the drive motor, and drives the support plate to rise or fall through the first bearing block;

[0014] The second side plate is provided with an auxiliary guide rail that matches the linear guide rail, and the second bearing block is matched with the auxiliary guide rail.

[0015] Optionally, the first type of reagent needle assembly includes a first support plate and a cleaning reagent needle fixedly installed below the first support plate;

[0016] The first support plate is fixedly installed on the first side plate and the second side plate by a plurality of support columns; the longitudinal axis of the cleaning reagent needle is parallel to the longitudinal axis of the support columns;

[0017] The first support plate, the plurality of the support columns, the first side plate, and the second side plate form a receiving space; the cleaning reagent needle is located in the receiving space.

[0018] The first support plate is provided with a rotary valve assembly that is connected to the cleaning reagent needle through a pipe.

[0019] Optionally, the first type of reagent kit component includes a pull-out box and a cleaning reagent kit disposed inside the pull-out box;

[0020] The cleaning reagent needle is configured to correspond to the sealing film of the cleaning kit;

[0021] The pull-out box has the degree of freedom to move along the longitudinal axis of the second type of reagent kit component.

[0022] Optionally, the second type of reagent needle assembly includes a second carrier plate and a sequencing reagent needle. The second carrier plate includes a horizontal portion and a connecting portion. The horizontal portion is arranged parallel to the first carrier plate and is fixedly installed on one side of the connecting portion.

[0023] The other side of the connecting part is fixedly connected to the first bearing plate;

[0024] The sequencing reagent needle is fixedly installed below the horizontal part;

[0025] The connecting part is provided with a plunger pump assembly that is connected to the sequencing reagent needle and the cleaning reagent needle through a pipe, and the sequencing reagent needle is connected to the rotary valve assembly through a pipe.

[0026] Optionally, the second type of reagent kit component includes a refrigerator and a sequencing reagent kit placed inside the refrigerator;

[0027] The sequencing reagent needle is configured to correspond to the sealing film of the sequencing kit;

[0028] The distance from the needle tip of the sequencing reagent needle to the bottom of the sequencing kit is equal to the distance from the needle tip of the cleaning reagent needle to the bottom of the cleaning kit.

[0029] Optionally, the refrigerator includes a box body and an openable / closeable door, and the top of the box body has a through hole that matches the sequencing reagent needle;

[0030] The top of the box is also provided with a slide rail that matches the bottom of the pull-out box. A limiting member is provided on the slide rail, and the limiting member is located between the pull-out box and the through hole.

[0031] Optionally, a first limiting block is provided at the end of the linear guide rail near the drive motor;

[0032] A second limiting block is provided at the end of the lead screw away from the drive motor;

[0033] The first limiting block and the second limiting block are respectively matched and arranged on both sides of the bearing plate to restrict the bearing plate from moving upward and downward, respectively.

[0034] Optionally, a first limit switch and a second limit switch are provided on the side of the first side plate;

[0035] Both the first limit switch and the second limit switch are matched with the bearing plate;

[0036] The distance between the first limit switch and the second limit switch is greater than the height of the sequencing kit.

[0037] Secondly, this application discloses a gene sequencing system, including the aforementioned reagent extraction mechanism.

[0038] The reagent extraction mechanism disclosed in this application, driven by a power unit, can control the synchronous movement of the second type reagent kit assembly, the first type reagent kit assembly relative to the first type reagent needle assembly, and the second type reagent needle assembly. Without moving the reagent needle, simply controlling the upward movement of the reagent kit enables the reagent needle to break the membrane of the reagent kit and extract the corresponding reagent. By controlling the downward movement of the reagent kit, it can detach from the reagent needle, effectively reducing operation time. The fixed position of the reagent needle assembly effectively shortens the length of the transmission tubing connected to the reagent needle, greatly reducing reagent waste in the tubing and lowering the fluid pressure requirement, achieving smooth, efficient, and accurate reagent extraction.

[0039] The above description is merely an overview of the technical solution disclosed herein. In order to better understand the technical means of this disclosure and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0040] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0041] Figure 1 This is a three-dimensional schematic diagram of a reagent extraction mechanism provided in an embodiment of this disclosure.

[0042] Figure 2 for Figure 1 Another perspective diagram.

[0043] Figure 3 for Figure 1 A three-dimensional schematic diagram of the central support frame.

[0044] Figure 4 for Figure 1 A three-dimensional schematic diagram of the power unit.

[0045] Explanation of reference numerals in the attached figures:

[0046] 110. Base plate; 120. First side plate; 130. Second side plate; 140. Support column; 210. Drive motor; 220. Lead screw; 310. Support plate; 311. First support block; 312. Support plate; 313. Second support block; 320. Linear guide rail; 331. First limit block; 332. Second limit block; 340. First limit switch; 350. Second limit switch; 410. First support plate; 420. Cleaning reagent needle; 500. First type of reagent kit assembly; 510. Slide rail; 520. Limiting component; 610. Second support plate; 611. Horizontal part; 612. Connecting part; 620. Sequencing reagent needle; 700. Second type of reagent kit assembly; 800. Rotary valve assembly; 900. Plunger pump assembly. Detailed Implementation

[0047] The present disclosure will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the disclosure. Furthermore, it should be noted that, for ease of description, only the parts relevant to the present disclosure are shown in the accompanying drawings.

[0048] It should be noted that, where there is no conflict, the embodiments and features described in this disclosure can be combined with each other. The technical solutions of this disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0049] Unless otherwise stated, the exemplary implementations / embodiments shown are to be understood as providing exemplary features of various details that provide ways in which the technical concepts of this disclosure can be implemented in practice. Therefore, unless otherwise stated, the features of various implementations / embodiments may be additionally combined, separated, interchanged and / or rearranged without departing from the technical concepts of this disclosure.

[0050] The use of crosshairs and / or shading in the accompanying drawings is generally used to clarify the boundaries between adjacent components. Thus, unless otherwise stated, the presence or absence of crosshairs or shading does not convey or indicate any preference or requirement for the specific material, material properties, dimensions, proportions, commonalities between the illustrated components, or any other characteristics, properties, etc., of the components. Furthermore, in the accompanying drawings, the dimensions and relative dimensions of components may be exaggerated for clarity and / or descriptive purposes. When exemplary embodiments can be implemented differently, a specific process sequence may be performed in a different order than that described. For example, two consecutively described processes may be performed substantially simultaneously or in the reverse order of their description. Furthermore, the same reference numerals denote the same components.

[0051] When a component is referred to as being "on" or "above" another component, "connected to," or "joined to" another component, the component may be directly on, directly connected to, or directly joined to the other component, or there may be intermediate components. However, when a component is referred to as being "directly on" another component, "directly connected to," or "directly joined to" another component, there are no intermediate components. Therefore, the term "connection" can refer to a physical connection, an electrical connection, etc., and may or may not have intermediate components.

[0052] For descriptive purposes, this disclosure may use spatial relative terms such as “below,” “under,” “below,” “down,” “above,” “above,” “higher,” and “side (e.g., in a “sidewall”)” to describe the relationship between one component and another component as shown in the accompanying drawings. In addition to the orientations depicted in the drawings, the spatial relative terms are also intended to encompass different orientations of the device during use, operation, and / or manufacture. For example, if the device in the drawings is flipped, a component described as “below” or “under” another component or feature would subsequently be positioned “above” said other component or feature. Thus, the exemplary term “below” can encompass both “above” and “below” orientations. Furthermore, the device may be otherwise positioned (e.g., rotated 90 degrees or in other orientations), thus interpreting the spatial relative descriptive terms used herein accordingly.

[0053] The terminology used herein is for the purpose of describing particular embodiments and is not intended to be limiting. As used herein, unless the context clearly indicates otherwise, the singular forms “a” and “the” are intended to include the plural forms as well. Furthermore, when the terms “comprising” and / or “including” and variations thereof are used in this specification, it indicates the presence of the stated features, integrals, steps, operations, parts, components, and / or groups thereof, but does not exclude the presence or addition of one or more other features, integrals, steps, operations, parts, components, and / or groups thereof. It should also be noted that, as used herein, the terms “substantially,” “about,” and other similar terms are used as approximate terms rather than as terms of degree, thus explaining the inherent biases in measurements, calculated values, and / or provided values ​​that would be recognized by one of ordinary skill in the art.

[0054] Reference Figure 1 and Figure 2This application discloses a reagent extraction mechanism, including a support frame and a power device installed on the support frame. The support frame is equipped with a first type of reagent needle assembly and a second type of reagent needle assembly arranged in a stepped manner. A first type of reagent kit assembly 500 and a second type of reagent kit assembly 700 are respectively arranged below the first type of reagent needle assembly and the second type of reagent needle assembly.

[0055] Specifically, the support frame includes a base plate 110, on which a first side plate 120 and a second side plate 130 are provided. The second side plate 130 is arranged opposite to the first side plate 120. The power unit is installed inside the first side plate 120. The first side plate 120 and the second side plate 130 serve as the bottom structure supporting the first type of reagent needle assembly and the second type of reagent needle assembly. The first type of reagent kit assembly 500 is located between the first type of reagent needle assembly and the base plate 110, and the second type of reagent kit assembly 700 is located between the second type of reagent needle assembly and the base plate 110. This design makes full use of space, meets the reagent extraction requirements, and makes full use of space, resulting in a compact structure.

[0056] The first reagent kit assembly 500 is mounted on top of the second reagent kit assembly 700, meaning that the first reagent kit assembly 500 and the second reagent kit assembly 700 move synchronously. The first reagent kit in the first reagent kit assembly 500 has the freedom to move along the longitudinal axis of the second reagent kit assembly 700, which facilitates the placement and removal of the first reagent kit.

[0057] In addition, the first type of reagent kit component 500 has the degree of freedom to move along the longitudinal axis of the second type of reagent kit component 700, which allows the reagent kit component to be flexibly adjusted according to different reagent extraction requirements, thereby increasing the adaptability and versatility of the system and meeting the extraction requirements of different types and quantities of reagent kits.

[0058] A support plate 310 is installed on the power output end of the power device, and the second type reagent kit component 700 is fixedly connected to the support plate 310; the support plate 310 has the freedom to move the second type reagent kit component 700 and the first type reagent kit component 500 up and down under the drive of the power device.

[0059] Driven by a power unit, the carrier plate 310 allows the reagent kit assembly to be precisely positioned below the reagent needle, enabling more accurate reagent extraction. This design effectively improves reagent extraction efficiency and reduces operation time and reagent waste.

[0060] The reagent extraction mechanism disclosed in this application, driven by a power unit, can control the synchronous movement of the second type reagent kit assembly, the first type reagent kit assembly relative to the first type reagent needle assembly, and the second type reagent needle assembly. Without moving the reagent needle, simply controlling the upward movement of the reagent kit enables the reagent needle to break the membrane of the reagent kit and extract the corresponding reagent. By controlling the downward movement of the reagent kit, it can detach from the reagent needle, effectively reducing operation time. The fixed position of the reagent needle assembly effectively shortens the length of the transmission tubing connected to the reagent needle, greatly reducing reagent waste in the tubing and lowering the fluid pressure requirement, achieving smooth, efficient, and accurate reagent extraction.

[0061] In this embodiment, the first type of reagent needle assembly is preferably a cleaning reagent needle assembly, and the second type of reagent needle assembly is preferably a sequencing reagent needle assembly.

[0062] Specifically, the first type of reagent needle assembly includes a first support plate 410 and a plurality of cleaning reagent needles 420 fixedly installed below the first support plate 410; the first support plate 410 is fixedly installed on the first side plate 120 and the second side plate 130 by a plurality of support columns 140, preferably four support columns 140, which are respectively set at the four corners of the first support plate 410 near the edge to achieve stable support for the first support plate 410.

[0063] The longitudinal axis of the cleaning reagent needle 420 is parallel to the longitudinal axis of the support column 140, ensuring the accurate positioning of the cleaning reagent needle 420.

[0064] The first support plate 410, several support columns 140, the first side plate 120, and the second side plate 130 form a receiving space, and several cleaning reagent needles 420 are located in this receiving space, so as to make full use of the space.

[0065] A rotary valve assembly 800 is provided on the first support plate 410 and connected to the cleaning reagent needle 420 through a pipe. By installing the rotary valve assembly 800 on the first support plate 410, a shorter transmission pipeline path is achieved to connect to several cleaning reagents, effectively reducing the pipeline length and thus reducing the waste of reagent residue in the pipeline.

[0066] The first type of reagent kit component 500 includes a pull-out box and a cleaning reagent kit (not shown in the figure) disposed inside the pull-out box. The pull-out box has the freedom to move along the longitudinal axis of the second type of reagent kit component 700, that is, the pull-out box can be pulled out and pushed in, which facilitates rapid replacement of the reagent kit. The pull-out box has several through holes that match several cleaning reagent needles 420, which facilitates the precise insertion of the cleaning reagent needles 420 during the upward movement of the first type of reagent kit component 500.

[0067] The cleaning reagent needles 420 are configured to correspond to the sealing film of the cleaning kit. Initially, the cleaning kit placed in the pull-out housing is sealed by the sealing film. Several cleaning reagent needles 420 can be located on the upper side of the pull-out housing or within the through-hole of the housing. When the first type of reagent kit assembly 500 is driven upwards, the cleaning reagent needles 420 extend through the through-hole into the pull-out housing. As the pull-out housing continues to rise, the cleaning reagent needles 420 pierce the sealing film of the cleaning kit and enter the interior of the cleaning kit.

[0068] In this embodiment, the pull-out box is preferably a drawer structure.

[0069] The second type of reagent needle assembly includes a second support plate 610 and a plurality of sequencing reagent needles 620. The second support plate 610 includes a horizontal part 611 and a connecting part 612 (i.e., a vertical part). The horizontal part 611 is arranged parallel to the first support plate 410 and is fixedly installed on one side of the connecting part 612. The other side of the connecting part 612 is fixedly connected to the first support plate 410. The plurality of sequencing reagent needles 620 are all fixedly installed below the horizontal part 611. A plunger pump assembly 900 is provided on the connecting part 612 and is connected to the sequencing reagent needles 620 and the cleaning reagent needles 420 through pipes. The sequencing reagent needles 620 are connected to the rotary valve assembly 800 through pipes. The plunger pump assembly 900 and the rotary valve assembly 800 control the reagent extraction action of the corresponding reagent needles.

[0070] By installing the plunger pump assembly 900 on the connecting part 612 and installing several sequencing reagent needles 620 below the horizontal part 611, a shorter path can be achieved for the transmission tubing connected to the several sequencing reagent needles 620 without interfering with the transmission tubing. By fixing the second type of reagent needle assembly to the first carrier plate 410, the second type of reagent needle assembly and the first type of reagent needle assembly are integrated into a synchronous whole. Through synchronous movement, rapid and precise control of the extraction of different types of reagents can be achieved.

[0071] The second type of reagent kit component 700 includes a refrigerator and a sequencing reagent kit (not shown in the figure) placed inside the refrigerator. The refrigerator includes a cabinet and an opening and closing door. Specifically, the opening and closing door is connected to the bottom of the cabinet and is rotatable relative to the connection point. When the opening and closing door is rotated outward, the cabinet is opened, facilitating the quick loading and unloading of the sequencing reagent kit. When the opening and closing door is rotated inward to the correct position, the cabinet is sealed to ensure the refrigeration effect inside the cabinet and provide the refrigeration environment required for the sequencing reagent kit.

[0072] Furthermore, the refrigerator is equipped with a locking mechanism that matches the sequencing kit. When placing the sequencing kit, simply open the door and push the kit in until it locks into place (meaning it can't be pushed any further). This indicates that the sequencing kit is in place, making the process simple, convenient, and effectively ensuring the accuracy of the sequencing kit placement each time.

[0073] The top of the box has several through holes that match the sequencing reagent needle 620, which facilitates the precise insertion of the sequencing reagent needle 620 during the ascent of the second type of reagent kit component 700.

[0074] The top of the box is also equipped with a slide rail 510 that matches the bottom of the pull-out box. The pull-out box can move back and forth along the slide rail 510 to facilitate the loading and unloading of the cleaning reagent kit. A limiting member 520 is provided on the slide rail 510. The limiting member 520 is located between the pull-out box and the through hole and is used to limit the pull-out box.

[0075] Specifically, there are two slide rails 510 to facilitate the quick pushing and pulling of the box. The sequencing reagent needle 620 matching through holes on the top of the box does not interfere with the two slide rails 510. In this embodiment, the sequencing reagent needle 620 matching through holes on the top of the box are located in the area between the two slide rails 510.

[0076] The sequencing reagent needles 620 are configured to correspond to the sealing film of the sequencing kit. Initially, the sequencing kit is sealed within the housing using the sealing film. Several sequencing reagent needles 620 can be located on the upper side of the housing or within the through-holes in the housing. When the second type of reagent kit assembly 700 is driven upwards, the sequencing reagent needles 620 extend through the through-holes into the housing. As the housing continues to rise, the sequencing reagent needles 620 pierce the sealing film of the sequencing kit and enter its interior.

[0077] In this embodiment, the distance from the needle tip of the sequencing reagent needle 620 to the bottom of the sequencing kit is equal to the distance from the needle tip of the cleaning reagent needle 420 to the bottom of the cleaning kit. This ensures that when the second type of kit component 700 and the first type of kit component 500 rise to the preset position simultaneously, the positions of the sequencing reagent needle 620 and the cleaning reagent needle 420 within the sequencing kit both meet the corresponding reagent extraction position requirements.

[0078] In this embodiment, the first type of reagent needle assembly and the second type of reagent needle assembly are arranged in a stepped manner, which can effectively reduce the lever arm of the power module, and at the same time make the installation of fluid components such as rotary valve and plunger pump more concentrated, making the entire reagent extraction mechanism more compact; at the same time, this arrangement allows the reagent needle assembly to more effectively cover multiple reagent kits, reducing the movement distance of the reagent needles, thereby reducing the length of the tubing.

[0079] Reference Figure 3 and Figure 4 The power unit includes a drive motor 210 and a lead screw 220 mounted on the power output end of the drive motor 210. The first side plate 120 is provided with a motor mounting part that matches the drive motor 210. The drive motor 210 is fixed to the motor mounting part by a motor bracket. The lead screw 220 is located inside the first side plate 120 and the lead screw 220 does not interfere with the first side plate 120.

[0080] A linear guide rail 320 is provided on the inner side of the first side plate 120. In this embodiment, two linear guide rails 320 are provided, and the two linear guide rails 320 are arranged in parallel. Preferably, the two guide rails are arranged symmetrically with respect to the lead screw 220.

[0081] The support plate 310 includes a first support block 311, a support plate 312, and a second support block 313. The first support block 311 and the second support block 313 are respectively fixedly disposed on both sides of the support plate 312. The second type of reagent kit assembly 700 is disposed on the top of the support plate 312. In order to ensure stability during the lifting process, the bottom of the second type of reagent kit assembly 700 can be fixedly connected to the support plate 312.

[0082] The first bearing block 311 is matched with the lead screw 220. Specifically, the first bearing block 311 has a threaded hole that matches the lead screw 220 for matching with the outer side of the lead screw 220. After the drive motor 210 is started, the drive motor 210 drives the lead screw 220 to rotate, converting the rotational motion into linear motion, thereby driving the bearing plate 310 to move up or down.

[0083] The first support block 311 is engaged with the linear guide rail 320. Specifically, the side of the first support block 311 has a groove that matches the protrusion on the linear guide rail 320. With this arrangement, the linear guide rail 320 can guide the movement of the first support block 311, ensuring precise vertical movement during the rising or falling process, preventing deviation, and thus ensuring precise control of the lifting and lowering positions of the first type reagent kit component 500 and the second type reagent kit group.

[0084] The second side plate 130 is provided with an auxiliary guide rail that matches the linear guide rail 320. The matching setting here can be understood as the auxiliary guide rail being set parallel to the linear guide rail 320. The specific number and setting position can correspond to the linear guide rail 320.

[0085] The second support block 313 is matched with the auxiliary guide rail. Specifically, the second support block 313 has a groove that matches the auxiliary guide rail. The groove engages with the protrusion on the auxiliary guide rail, and the movement of the second support block 313 on the auxiliary guide rail is synchronized with the movement of the support plate 312. When the drive motor 210 is started, the lead screw 220 rotates under the drive of the drive motor 210, which drives the support plate 312 to rise or fall through the first support block 311, that is, drives the first type of reagent kit assembly 500 and the second type of reagent kit assembly to rise or fall, thereby achieving precise control of the rising or falling position of the reagent kit assembly.

[0086] In this embodiment, an auxiliary guide rail is preferably provided, which can be symmetrically arranged with the lead screw 220.

[0087] The first bearing block 311 and the second bearing block 313 are preferably L-shaped structures. The horizontal part 611 of the two L-shaped structures is located below the support plate 312 and is fixed to the support plate 312 to support the support plate 312.

[0088] The end of the linear guide 320 near the drive motor 210 is provided with a first limiting block 331 for limiting the upward movement of the first bearing block 311. The number of first limiting blocks 331 can be the same as the number of linear guides 320.

[0089] The end of the lead screw 220 away from the drive motor 210 is provided with a second limiting block 332 for limiting the downward movement of the first bearing block 311. The second limiting block 332 is provided with a bearing component that matches the end of the lead screw 220.

[0090] The side of the first side plate 120 is provided with a first limit switch 340 and a second limit switch 350. The first limit switch 340 and the second limit switch 350 are matched with the first bearing block 311 in the bearing support plate 310, so that the position of the first bearing block 311 rising or falling under the drive of the lead screw 220 can be obtained in time.

[0091] The distance between the first limit switch 340 and the second limit switch 350 is greater than the height of the sequencing kit, ensuring that the corresponding reagent needle will not puncture the sealing film on the kit in the initial state.

[0092] When the sequencing kit rises to the first preset position, the first limit switch 340 is triggered, controlling the drive motor 210 to stop. The first preset position includes the sequencing reagent needle 620 having its nozzle inside the sequencing kit, and the distance between the bottom of the sequencing kit and the nozzle of the sequencing reagent needle 620 meeting the first preset distance. The first preset distance can be either contact or close proximity.

[0093] When the sequencing kit descends to the second preset position, the second limit switch 350 is triggered, controlling the drive motor 210 to stop. The second preset position includes the sequencing reagent needle 620 having its nozzle outside the sequencing kit, and the distance between the top of the sequencing kit and the nozzle of the sequencing reagent needle 620 meeting the second preset distance. The second preset distance is when the nozzle of the sequencing reagent needle 620 is detached from the sequencing kit.

[0094] Secondly, this application discloses a gene sequencing system, including the aforementioned reagent extraction mechanism, which uses a drive motor to drive a lead screw to rotate, thereby driving the sequencing reagent kit and the cleaning reagent kit to move up and down, thereby achieving membrane breaking and reagent extraction.

[0095] In the description of this specification, the references to terms such as "one embodiment / mode," "some embodiments / modes," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment / mode or example is included in at least one embodiment / mode or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment / mode or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments / modes or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments / modes or examples described in this specification, as well as the features of different embodiments / modes or examples.

[0096] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0097] Those skilled in the art should understand that the above embodiments are merely for illustrating the present disclosure and are not intended to limit the scope of the disclosure. Those skilled in the art can make other changes or modifications based on the above disclosure, and these changes or modifications still fall within the scope of the present disclosure.

Claims

1. A reagent extraction mechanism, characterized by, The utility model relates to a reagent needle assembly and reagent box assembly for nucleic acid sequencing, and belongs to the field of nucleic acid sequencing. The utility model discloses a reagent needle assembly and reagent box assembly for nucleic acid sequencing, which comprises a bearing frame, a first type of reagent needle assembly and a second type of reagent needle assembly arranged in a stepped manner are mounted on the bearing frame, a first type of reagent box assembly and a second type of reagent box assembly are correspondingly arranged below the first type of reagent needle assembly and the second type of reagent needle assembly, the first type of reagent box assembly is mounted on the top of the second type of reagent box assembly, and the first type of reagent box in the first type of reagent box assembly has the freedom of moving along the longitudinal axis of the second type of reagent box assembly, a power device is arranged on the bearing frame, a bearing pallet is mounted on the power output end of the power device, the second type of reagent box assembly is fixedly connected with the bearing pallet, and the bearing pallet has the freedom of lifting the second type of reagent box assembly and the first type of reagent box assembly under the drive of the power device. The power device comprises a driving motor and a lead screw assembled on the power output end of the driving motor. The bearing frame comprises a bottom plate, a first side plate and a second side plate are arranged on the bottom plate, and the second side plate is oppositely arranged with the first side plate. The first side plate is provided with a motor mounting portion matched with the driving motor and a linear guide rail.

2. The reagent extraction mechanism of claim 1, wherein, The bearing pallet comprises a first bearing block, a second bearing block and a support plate, the second type of reagent box assembly is arranged on the top of the support plate, and the first bearing block and the second bearing block are fixedly arranged on the two sides of the support plate. The first bearing block is engaged with the linear guide rail, and the first bearing block is matched with the lead screw; the lead screw rotates under the drive of the driving motor, and the support plate is lifted or lowered through the first bearing block. The second side plate is provided with an auxiliary guide rail matched with the linear guide rail, and the second bearing block is matched with the auxiliary guide rail. The first type of reagent needle assembly comprises a first bearing plate and a cleaning reagent needle fixedly mounted below the first bearing plate. The first bearing plate is fixedly mounted on the first side plate and the second side plate through a plurality of support columns, and the longitudinal axis of the cleaning reagent needle is parallel to the longitudinal axis of the support column. The first bearing plate, the plurality of support columns, the first side plate and the second side plate form an accommodation space, and the cleaning reagent needle is located in the accommodation space.

3. The reagent extraction mechanism of claim 2, wherein, The first bearing plate is provided with a rotary valve assembly connected with the cleaning reagent needle through a pipeline. The first type of reagent box assembly comprises a pull-out box body and a cleaning reagent box arranged in the pull-out box body. The cleaning reagent needle is correspondingly arranged with the sealing film of the cleaning reagent box. The pull-out box body has the freedom of moving along the longitudinal axis of the second type of reagent box assembly.

4. The reagent extraction mechanism of claim 3, wherein, The second type of reagent needle assembly comprises a second bearing plate and a sequencing reagent needle, the second bearing plate comprises a horizontal part and a connecting part, the horizontal part is arranged in parallel with the first bearing plate, and the horizontal part is fixedly mounted on one side of the connecting part. The other side of the connecting part is fixedly connected with the first bearing plate. The sequencing reagent needle is fixedly mounted below the horizontal part.

5. The reagent extraction mechanism of claim 4, wherein, ​ ​ ​ The connecting part is provided with a plunger pump assembly connected with the sequencing reagent needle and the cleaning reagent needle through pipelines, and the sequencing reagent needle is connected with the rotary valve assembly through pipelines.

6. The reagent extraction mechanism of claim 5, wherein, The second type of kit assembly comprises a refrigeration box and a sequencing reagent kit placed in the refrigeration box. The sequencing reagent needle is correspondingly provided with a sealing film of the sequencing reagent kit. The distance from the needle opening of the sequencing reagent needle to the bottom of the sequencing reagent kit is equal to the distance from the needle opening of the cleaning reagent needle to the bottom of the cleaning reagent kit.

7. The reagent extraction mechanism of claim 6, wherein, The refrigeration box comprises a box body and an opening and closing door, and the top of the box body is provided with a through hole matched with the sequencing reagent needle. The top of the box body is further provided with a sliding rail matched with the bottom of the pull-out box body, and the sliding rail is provided with a limiting piece between the pull-out box body and the through hole.

8. The reagent extraction mechanism of claim 2, wherein, The end of the linear guide rail close to the driving motor is provided with a first limiting block. The end of the lead screw away from the driving motor is provided with a second limiting block. The first limiting block and the second limiting block are respectively matched with the two sides of the bearing tray to limit the upward movement and downward movement of the bearing tray.

9. The reagent extraction mechanism of claim 6, wherein, The side of the first side plate is provided with a first limiting switch and a second limiting switch. The first limiting switch and the second limiting switch are matched with the bearing tray. The distance between the first limiting switch and the second limiting switch is greater than the height of the sequencing reagent kit.

10. A genetic sequencing system characterized by, The reagent extraction mechanism comprises the kit of any one of claims 1-9.

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