Exosome extracting and filtering column

Abstract

The utility model relates to the technical field of exosome extraction, and discloses an exosome extraction filter column which comprises a filter cartridge, a filter element is mounted at the lower part of the inner wall of the filter cartridge, and a liquid discharge head is mounted at the lower end of the filter cartridge; a cover body is mounted at the top of the filter cartridge, a tubular ejector rod slidably penetrates through the middle of the cover body, a pressing block is mounted on the outer side of the top of the tubular ejector rod, a push block is mounted on the outer side of the bottom of the tubular ejector rod, a sealing gasket sleeves the push block, a guide hole communicated with the tubular ejector rod is formed in the center of the push block, and an electric control valve is mounted at the lower part of the tubular ejector rod; a liquid level sensor is installed on the inner side of the lower portion of the tubular ejector rod. Compared with the prior art, the utility model has the advantages that air in the filter cartridge can be exhausted through the guide hole in the center of the push block and the tubular ejector rod, so that the air in the filter cartridge is prevented from influencing the pressing effect of the piston of the filter column and the collection of the exosome; and through cooperation of the liquid level sensor, the electric control valve and the control switch, automatic closing of the tubular ejector rod is achieved, and operation is convenient.

Description

Technical Field

[0001] This utility model relates to the field of exosome extraction technology, specifically to an exosome extraction filter column. Background Technology

[0002] Exosomes are phospholipid-membrane secretory vesicles with a diameter between 30 and 200 nm. They bud from the cell membrane and endosome membrane and are rich in specific proteins, lipids, nucleic acids, and glycoconjugates. The expression levels of exosome contents are closely related to cell type and cellular health or disease status. Exosomes also play a significant role in intercellular communication and disease diagnosis.

[0003] Research revealed the following shortcomings of existing exosome extraction filter columns: when adding the stock solution into the filter cartridge, it is not easy to completely remove the air from the extraction device, which not only affects the downward pressure of the filter column piston, but also the expelled air during excretion can easily affect the collection of exosomes.

[0004] To address the aforementioned shortcomings, this application proposes an exosome extraction filter column. Utility Model Content

[0005] I. Technical problems to be solved

[0006] The technical problem this invention aims to solve is that when adding raw liquid into the filter cartridge, it is not easy to completely remove the air from the extraction device, which affects the downward pressure of the filter column piston and the collection of exosomes.

[0007] II. Technical Solution

[0008] To solve the above-mentioned technical problems, the technical solution provided by this utility model is: an exosome extraction filter column, including a filter cylinder, a filter element installed on the lower part of the inner wall of the filter cylinder, and a drain head installed at the lower end of the filter cylinder;

[0009] The filter cartridge is topped with a cover, through which a tubular push rod slides. A pressing block is mounted on the outer side of the top of the tubular push rod, and a push block is mounted on the outer side of the bottom of the tubular push rod. A sealing gasket is fitted on the outer side of the push block, and a guide hole communicating with the tubular push rod is provided at the center of the push block. An electric control valve is mounted on the lower part of the tubular push rod, and a liquid level sensor is mounted on the inner side of the lower part of the tubular push rod, below the electric control valve.

[0010] As an improvement, a battery box is embedded in the upper side of the cover, and a control switch is installed above the battery box.

[0011] As an improvement, the control switch, in conjunction with a spiral wire, is connected to the level sensor and the electric control valve, respectively.

[0012] As an improvement, a warning light is installed on the outer wall of the cover, and the warning light is connected to the control switch with a wire.

[0013] As an improvement, the two ends of the filter cylinder are respectively connected to the cover and the drain head with threads.

[0014] As an improvement, a support assembly is installed on the outside of the filter cartridge. The support assembly includes a base plate and a support ring. The support ring is located on the outside of the middle part of the filter cartridge, and multiple sets of support legs are connected between the support ring and the base plate.

[0015] As an improvement, a limiting ring is fixedly installed on the outer side of the middle part of the filter cylinder, and an annular groove is provided on the upper part of the inner wall of the support ring to cooperate with the limiting ring. Multiple sets of limiting rods are installed on the lower side of the limiting ring, and multiple sets of insertion holes for the limiting rods are provided at the bottom of the annular groove.

[0016] III. Beneficial Effects

[0017] The advantages of this invention compared with the prior art are as follows: after the exosome stock solution is put into the filter cartridge, the air in the filter cartridge can be discharged through the guide hole in the center of the push block and the tubular push rod, so as to prevent the air in the filter cartridge from affecting the downward pressing effect of the filter column piston and the collection of exosomes.

[0018] The tubular push rod is automatically closed by the cooperation of a liquid level sensor, an electric control valve, and a control switch, making it easy to operate. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of an exosome extraction filter column according to this utility model.

[0020] Figure 2 This is a schematic diagram of the filter cylinder structure of an exosome extraction filter column according to this utility model.

[0021] Figure 3 This is a schematic diagram of the cover structure of an exosome extraction filter column according to the present invention.

[0022] Figure 4 This is a schematic diagram of the tubular top rod connection structure of an exosome extraction filter column according to this utility model.

[0023] Figure 5 This is a schematic diagram of the lower side structure of the pusher block of an exosome extraction filter column according to this utility model.

[0024] Figure 6 This is a schematic diagram of the support component structure of an exosome extraction filter column according to the present invention.

[0025] As shown in the figure: 1. Filter cylinder; 2. Filter element; 3. Cover; 4. Tubular push rod; 5. Push block; 6. Sealing gasket; 7. Guide hole; 8. Electric control valve; 9. Liquid level sensor; 10. Pressing block; 11. Battery box; 12. Control switch; 13. Warning light; 14. Spiral wire; 15. Drain head; 16. Base plate; 17. Support ring; 18. Support leg; 19. Limiting ring; 20. Annular groove; 21. Limiting rod; 22. Insertion hole. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0027] Example 1

[0028] As attached Figure 1 Appendix Figure 2 Appendix Figure 3 and attached Figure 5 As shown, an exosome extraction filter column includes a filter cylinder 1, a filter element 2 installed on the lower part of the inner wall of the filter cylinder 1, a drain head 15 installed at the lower end of the filter cylinder 1, and a cover 3 installed at the top of the filter cylinder 1. The two ends of the filter cylinder 1 are respectively threaded to the cover 3 and the drain head 15, which facilitates the installation and removal of the cover 3 and the drain head 15 from the filter cylinder 1. A tubular push rod 4 slides through the middle of the cover 3. A pressing block 10 is installed on the outer side of the top of the tubular push rod 4, and a push block 5 is installed on the outer side of the bottom of the tubular push rod 4. A sealing gasket 6 is sleeved on the outer side of the push block 5, and a guide hole 7 communicating with the tubular push rod 4 is provided at the center of the push block 5.

[0029] With the above structure, the cover 3 is removed from the top of the filter cylinder 1, the original liquid is sent into the filter cylinder 1, the push block 5 is placed into the filter cylinder 1, the sealing gasket 6 is in sealing contact with the inner wall of the filter cylinder 1, the cover 3 is installed on the top of the filter cylinder 1, the tubular top rod 4 is positioned, the pressing block 10 is pressed down, and the pressing block 10 pushes the tubular top rod 4 downward, so that the push block 5 and the sealing gasket 6 move downward inside the filter cylinder 1, and the air inside the filter cylinder 1 is discharged through the guide hole 7 and the tubular top rod 4.

[0030] As attached Figure 1 Appendix Figure 3 and attached Figure 4As shown, an electric control valve 8 is installed at the lower part of the tubular top rod 4, and a liquid level sensor 9 is installed on the inner side of the lower part of the tubular top rod 4 and below the electric control valve 8. A battery box 11 is embedded in the upper side of the cover 3, and a control switch 12 is installed above the battery box 11. The control switch 12, together with the spiral wire 14, is connected to the liquid level sensor 9 and the electric control valve 8 respectively.

[0031] With the above structure, during the downward movement of push block 5 and sealing gasket 6, the raw liquid enters the guide hole 7 and then enters the tubular push rod 4. The liquid level is detected by the liquid level sensor 9, and the detected data is sent to the control switch 12 through the spiral wire 14. The control switch 12 sends a shut-off signal to the electric control valve 8, which closes the electric control valve 8 in time. At this time, the air inside the filter cartridge 1 and located below push block 5 is squeezed out, which continues to push push block 5 and sealing gasket 6 downward, squeezing the raw liquid and filtering it through filter element 2. The filtered exosomes flow into the drain head 15 and are sent to the collection bottle through the drain head 15.

[0032] Combined with appendix Figure 3 As shown, a warning light 13 is installed on the outer wall of the cover 3, and the warning light 13 is connected to the control switch 12 with a wire.

[0033] With the above structure, when the liquid level sensor 9 detects the raw liquid, the control switch 12 will light up the warning light 13 to remind the staff that squeezing filtration can be performed.

[0034] Example 2

[0035] Based on Example 1, please refer to the appendix. Figure 1 Appendix Figure 2 and attached Figure 6 A support assembly is installed on the outside of the filter cylinder 1. The support assembly includes a base plate 16 and a support ring 17. The support ring 17 is located on the outside of the middle part of the filter cylinder 1. Multiple sets of support legs 18 are connected between the support ring 17 and the base plate 16.

[0036] A limiting ring 19 is fixedly installed on the outer side of the middle part of the filter cylinder 1. An annular groove 20 that matches the limiting ring 19 is provided on the upper part of the inner wall of the support ring 17. Multiple sets of limiting rods 21 are installed on the lower side of the limiting ring 19. Multiple sets of insertion holes 22 that match the insertion of the limiting rods 21 are provided at the bottom of the annular groove 20.

[0037] With the structure described in Embodiment 2, the filter cartridge 1 is prone to tilting when operated by hand, which affects the accuracy of the liquid level sensor 9. The filter cartridge 1 is inserted into the support ring 17, the limiting ring 19 is inserted into the annular groove 20, and the limiting rod 21 is inserted into the insertion hole 22 to position the filter cartridge 1. A distance is left at the bottom of the drain head 15 to facilitate the collection of exosomes.

[0038] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

[0040] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. An exosome extraction filter column, comprising a filter cartridge (1), a filter core (2) is mounted on the lower part of the inner wall of the filter cartridge (1), and a liquid discharge head (15) is mounted on the lower end of the filter cartridge (1), characterized in that: a cover (3) is mounted on the top of the filter cartridge (1), a tubular jacking rod (4) is slid through the middle of the cover (3), a pressing block (10) is mounted on the outside of the top of the tubular jacking rod (4), a push block (5) is mounted on the outside of the bottom of the tubular jacking rod (4), a sealing washer (6) is sleeved on the outside of the push block (5), a guide hole (7) is arranged in the center of the push block (5) and communicates with the tubular jacking rod (4), an electric control valve (8) is mounted on the lower part of the tubular jacking rod (4), and a liquid level sensor (9) is mounted on the inner side of the lower part of the tubular jacking rod (4) and below the electric control valve (8).

2. The exosome extraction filter column of claim 1, wherein: A battery box (11) is embedded and mounted on the upper side of the cover (3), and a control switch (12) is mounted above the battery box (11).

3. The exosome extraction filter column of claim 2, wherein: The control switch (12) is connected with the liquid level sensor (9) and the electric control valve (8) through a spiral wire (14).

4. The exosome extraction filter column of claim 2, wherein: A warning light (13) is mounted on the outer wall of the cover (3) and connected with the control switch (12) through a wire.

5. The exosome extraction filter column of claim 1, wherein: The filter cartridge (1) is connected with the cover (3) and the liquid discharge head (15) through threads at both ends.

6. The exosome extraction filter column of claim 1, wherein: A support assembly is mounted on the outer side of the filter cartridge (1), the support assembly comprises a bottom plate (16) and a support ring (17), the support ring (17) is located on the outer side of the middle part of the filter cartridge (1), and a plurality of support legs (18) are connected between the support ring (17) and the bottom plate (16).

7. The exosome extraction filter column of claim 6, wherein: A limiting ring (19) is fixedly mounted on the outer side of the middle part of the filter cartridge (1), an annular clamping groove (20) is arranged on the upper part of the inner wall of the support ring (17) and matched with the limiting ring (19), a plurality of limiting insertion rods (21) are mounted on the lower side of the limiting ring (19), and a plurality of insertion holes (22) are arranged at the bottom of the annular clamping groove (20) and matched with the insertion of the limiting insertion rods (21).

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