Nuclease dissolving device

Abstract

The utility model discloses a nuclease dissolving device which comprises a dissolving tank, a top cover mounted at the top of the dissolving tank, and a feeding conical cylinder mounted on the top cover, an opening and closing mechanism for controlling the feeding rate is arranged in the feeding conical cylinder; a material distributing and stirring mechanism is arranged in the dissolving tank; the distributing and stirring mechanism comprises a servo motor mounted at the bottom of the dissolving tank and a rotating rod fixed at the output end of the servo motor; after nuclease powder enters the dissolving tank from the feeding conical cylinder, through cooperation of the servo motor, the rotating rod, the dispersing circular truncated cone, the arc-shaped dispersing groove and the stirring blades, it can be ensured that the powder uniformly covers the surfaces of different positions of a buffer solution in a dispersed state, and then through mixing and stirring of the multiple stirring blades, the nuclease powder can be dissolved in the dissolving tank. The dissolving efficiency and the uniformity of the nuclease are remarkably improved, and the enzyme activity loss and the purity reduction caused by agglomeration are effectively prevented, so that the subsequent application effect of the nuclease is improved.

Description

Technical Field

[0001] This utility model relates to the field of nuclease technology, and in particular to a nuclease dissolution device. Background Technology

[0002] In the field of biotechnology, nucleases, as an important class of enzymes, are widely used in molecular biology experiments, genetic engineering, disease diagnosis and treatment, and many other areas. Nucleases can specifically cleave DNA or RNA molecules, playing a crucial role in biological processes such as DNA replication, repair, recombination, and gene expression regulation.

[0003] In the preparation of nucleases, nuclease powder needs to be dissolved in a specific buffer solution to achieve a suitable concentration and activity. However, traditionally, when dissolving both, the nuclease powder is often directly added to the buffer solution in one go or in large quantities. Due to the differences in density and solubility of nuclease powder, adding a large amount at once can easily lead to local accumulation of powder in the buffer solution, forming aggregates that are difficult to disperse. This not only slows down the dissolution rate, but may also prevent the nuclease molecules within the aggregates from fully contacting water molecules due to steric hindrance, thus affecting the dissolution efficiency and subsequent application effects. Summary of the Invention

[0004] This invention overcomes the shortcomings of the prior art and provides a nuclease dissolution device.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows: a nuclease dissolving device, comprising: a dissolving tank, a top cover installed on the top of the dissolving tank, and a feeding cone installed on the top cover; the feeding cone is provided with an opening and closing mechanism for controlling the feeding rate;

[0006] The dissolving tank is equipped with a material dispensing and stirring mechanism; the material dispensing and stirring mechanism includes: a servo motor installed at the bottom of the dissolving tank, a rotating rod fixed at the output end of the servo motor, and a dispersing frustum fixed at the top of the rotating rod;

[0007] The interior of the dispersing truncated cone has several arc-shaped dispersing grooves, and several connecting rings are installed on the side of the rotating rod. Several stirring blades are fixed on the side of the connecting rings.

[0008] In a preferred embodiment of this utility model, the top of the dispersing truncated cone is close to the bottom of the feeding cone, and the two are coaxially arranged; the side of the rotating rod near the bottom end is rotatably connected to the bottom of the dissolving tank, and a plurality of stirring blades are evenly distributed circumferentially on the side of the connecting ring.

[0009] In a preferred embodiment of this utility model, the opening and closing mechanism includes: a movable groove formed inside the straight section of the feed cone; a rotating ring rotatably connected inside the movable groove; and a plurality of opening and closing flaps hinged to the middle of the movable groove. A plurality of connecting blocks are fixed inside the rotating ring, and a plurality of connecting rods are hinged between the plurality of connecting blocks and the plurality of opening and closing flaps. A power source for driving the rotating ring to rotate is provided on the side of the rotating ring, and a limiting member for limiting the rotation of the rotating ring.

[0010] In a preferred embodiment of this utility model, the plurality of hinges are arranged in a circle, one side of the hinge is hinged to the inner side of the movable groove, the inner side of the feed cone near the movable groove abuts against the side of the plurality of hinges, and the movable groove and the rotating ring are coaxially arranged with the feed cone.

[0011] In a preferred embodiment of this utility model, the power source is one of an electric cylinder, a pneumatic cylinder, or a hydraulic cylinder; one end of the power source is hinged to the inner side of the movable groove, and the other end is hinged to the side of the rotating ring.

[0012] In a preferred embodiment of the present invention, the limiting member includes: a plurality of arc-shaped grooves formed on the surface of the rotating ring, and a plurality of convex locking blocks engaged with the inner side of the plurality of arc-shaped grooves; one end of the plurality of convex locking blocks is fixed to the inner side of the movable groove.

[0013] In a preferred embodiment of the present invention, a liquid inlet pipe is fixed to the top of the top cover, and the interior of the liquid inlet pipe extends to the lower surface of the top cover.

[0014] In a preferred embodiment of the present invention, a discharge cylinder is fixed to the bottom of the dissolving tank, the interior of the discharge cylinder extends into the interior of the dissolving tank, and a valve is installed on the side of the discharge cylinder.

[0015] In a preferred embodiment of this invention, the side of the dissolving tank is provided with an observation window for observing the internal condition of the dissolving tank.

[0016] In a preferred embodiment of the present invention, the bottom of the dissolving tank is provided with a support frame for supporting the vessel.

[0017] This utility model solves the defects existing in the background technology, and has the following beneficial effects:

[0018] (1) This utility model provides a nuclease dissolution device. By setting a material distribution and stirring mechanism inside the dissolution tank, after the nuclease powder enters the dissolution tank through the feeding cone, the servo motor, rotating rod, dispersing frustum, arc-shaped dispersing groove and stirring blades can ensure that the powder is in a dispersed state and evenly covers the surface of different positions of the buffer solution. Then, through the mixing and stirring of several stirring blades, not only is the dissolution efficiency and uniformity of the nuclease significantly improved, but also the enzyme activity loss and purity reduction caused by agglomeration are effectively prevented, thereby improving the subsequent application effect of the nuclease.

[0019] (2) In this utility model, by setting an opening and closing mechanism inside the feeding cone, when nuclease powder is added to the inside of the feeding cone, the nuclease powder can be fed through the straight section of the feeding cone by the cooperation of the power source, rotating ring, connecting block, connecting rod, opening and closing flaps and limiting parts. By limiting the rotation angle of the rotating ring, the degree of opening and closing of several opening and closing flaps can be controlled, so as to control the feeding rate of nuclease powder, ensure the stability and efficiency of the dissolution process, and thus improve the controllability and flexibility of feeding. Attached Figure Description

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0021] Figure 1 This is a perspective structural diagram of a preferred embodiment of the present invention;

[0022] Figure 2 This is a half-sectional view of the dissolving tank according to a preferred embodiment of the present invention;

[0023] Figure 3 This is a bottom view of the preferred embodiment of the present invention, showing the structure of the dispersed frustum.

[0024] Figure 4 This is a half-sectional view of the straight section of the feed cone according to a preferred embodiment of the present invention;

[0025] Figure 5 This is a structural diagram showing the distribution of several hinges in a preferred embodiment of the present invention;

[0026] In the diagram: 1. Dissolving tank; 11. Top cover; 12. Feed cone; 2. Servo motor; 21. Rotating rod; 22. Dispersion frustum; 23. Arc-shaped dispersion groove; 24. Connecting ring; 25. Stirring blade; 3. Movable groove; 31. Rotating ring; 32. Hinge; 33. Connecting block; 34. Connecting rod; 35. Power source; 4. Arc-shaped slide; 41. Convex locking block; 5. Liquid inlet pipe; 6. Discharge cylinder; 61. Valve; 7. Observation window; 8. Support frame. Detailed Implementation

[0027] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0028] like Figure 1 , Figure 2 and Figure 3 As shown, a nuclease dissolution device includes: a dissolution tank 1, a top cover 11 installed on the top of the dissolution tank 1, and a feed cone 12 installed on the top cover 11; the feed cone 12 is provided with an opening and closing mechanism for controlling the feed rate; the dissolution tank 1 is provided with a material dispensing and stirring mechanism; the material dispensing and stirring mechanism includes: a servo motor 2 installed at the bottom of the dissolution tank 1, a rotating rod 21 fixed to the output end of the servo motor 2, and a dispersing frustum 22 fixed to the top of the rotating rod 21; the dispersing frustum 22 has several arc-shaped dispersing grooves 23 inside, several connecting rings 24 are installed on the side of the rotating rod 21, and several stirring blades 25 are fixed on the side of the connecting rings 24.

[0029] It should be noted that the top of the dispersing frustum 22 is close to the bottom of the feed cone 12, and the two are coaxially arranged; the side of the rotating rod 21 near the bottom is rotatably connected to the bottom of the dissolving tank 1, and several stirring blades 25 are evenly distributed circumferentially on the side of the connecting ring 24; when the buffer solution is added into the dissolving tank 1, before adding the nuclease powder, the rotating rod 21 is driven to rotate by the servo motor 2, which can drive the dispersing frustum 22 and the stirring blades 25 to move. After the nuclease powder enters the dissolving tank 1 from the feed cone 12, it will fall onto the top of the dispersing frustum 22, and the several arc-shaped dispersing grooves 23 inside the dispersing frustum 22, through the rotational force, ensure that the powder is dispersed and evenly covers the surface of different positions of the buffer solution. Then, through the mixing and stirring of several stirring blades 25, not only is the dissolution efficiency and uniformity of the nuclease significantly improved, but also the enzyme activity loss and purity reduction caused by agglomeration are effectively prevented, thereby improving the subsequent application effect of the nuclease.

[0030] like Figure 4 and Figure 5 As shown, in some embodiments, the opening and closing mechanism includes: a movable groove 3 formed inside the straight section of the feed cone 12, a rotating ring 31 rotatably connected inside the movable groove 3, and a plurality of opening and closing flaps 32 hinged to the middle of the movable groove 3; a plurality of connecting blocks 33 are fixed inside the rotating ring 31, a plurality of connecting rods 34 are hinged between the plurality of connecting blocks 33 and the plurality of opening and closing flaps 32, and a power source 35 for driving the rotating ring 31 to rotate is provided on the side of the rotating ring 31, as well as a limiting member for limiting the rotation of the rotating ring 31.

[0031] It should be noted that the hinges 32 are arranged in a circle, with one side of each hinge 32 hinged to the inner side of the movable groove 3. The inner side of the feed cone 12 near the movable groove 3 abuts against the sides of the hinges 32. The movable groove 3 and the rotating ring 31 are coaxially arranged with the feed cone 12. When nuclease powder is added into the feed cone 12, the rotating ring 31 is driven to rotate by the power source 35. This causes the connecting blocks 33 on the inner side of the rotating ring 31 to open the hinges 32 via the connecting rod 34. This allows the nuclease powder to be fed through the straight section of the feed cone 12. By limiting the rotation angle of the rotating ring 31, the degree of opening and closing of the hinges 32 can be controlled, thereby controlling the rate of nuclease powder dispensing and ensuring the stability and efficiency of the dissolution process. This improves the controllability and flexibility of the feeding process.

[0032] In some embodiments, the power source 35 is one of an electric cylinder, a pneumatic cylinder, or a hydraulic cylinder; one end of the power source 35 is hinged to the inner side of the movable groove 3, and the other end is hinged to the side of the rotating ring 31; the way in which the power source 35 drives the rotating ring 31 to rotate as the power for the rotation of the rotating ring 31 can be achieved by technical means known to those skilled in the art, and therefore will not be described in detail here.

[0033] In some embodiments, the limiting member includes: a plurality of arc-shaped grooves 4 formed on the surface of the rotating ring 31, and a plurality of convex locking blocks 41 engaged with the inner side of the plurality of arc-shaped grooves 4; one end of the plurality of convex locking blocks 41 is fixed to the inner side of the movable groove 3.

[0034] It should be noted that the side with the smaller diameter of the convex locking block 41 contacts the inner side of the arc-shaped slide groove 4, while the side with the larger diameter is located on one side of the rotating ring 31. The number of arc-shaped slide groove 4, convex locking block 41, hinge 32, connecting block 33, and connecting rod 34 are the same. When the rotating ring 31 rotates, the arc-shaped slide groove 4 slides along the side of the convex locking block 41 to limit its movement. This design ensures that the hinge 32 can be opened and closed accurately to the designated position, avoiding malfunctions or damage caused by excessive or insufficient rotation, thereby providing a stable limiting effect on the rotation of the rotating ring 31.

[0035] like Figure 1 As shown, in some embodiments, a liquid inlet pipe 5 is fixed to the top of the top cover 11, and the interior of the liquid inlet pipe 5 extends to the lower surface of the top cover 11; the liquid inlet pipe 5 facilitates the addition of buffer solution for nuclease dissolution into the dissolution tank 1.

[0036] In some embodiments, a discharge cylinder 6 is fixed to the bottom of the dissolving tank 1, the interior of the discharge cylinder 6 extends into the interior of the dissolving tank 1, and a valve 61 is installed on the side of the discharge cylinder 6; the valve 61 is used to control the opening and closing of the discharge cylinder 6, and the discharge cylinder 6 is used to discharge the dissolved nuclease from the dissolving tank 1.

[0037] In some embodiments, the side of the dissolving tank 1 is provided with an observation window 7 for observing the internal condition of the dissolving tank 1; the observation window 7 allows the operator to observe the internal condition of the dissolving tank 1 without opening it, which facilitates timely detection and problem solving and ensures the smooth progress of the preparation process.

[0038] In some embodiments, a support frame 8 is provided at the bottom of the dissolving tank 1 to provide support; by supporting the dissolving tank 1 with the support frame 8, the weight of the dissolving tank 1 and its internal raw materials can be effectively distributed and borne, ensuring its stability and safety during the preparation process.

[0039] In use, an appropriate amount of buffer solution is added to the dissolving tank 1 through the inlet pipe 5 at the top of the top cover 11. The servo motor 2, installed at the bottom of the dissolving tank 1, is then activated, driving the rotating rod 21 and its top-mounted dispersing frustum 22 and stirring blade 25 to rotate. Before the nuclease powder is added, the pre-rotation of the dispersing frustum 22 and stirring blade 25 helps to create initial flow in the buffer solution, preparing for subsequent powder dispersion. Next, the nuclease powder is added through the feed cone 12. At this time, the power source 35 in the opening and closing mechanism drives the rotating ring 31 to rotate, which in turn drives the opening and closing flap 32 to gradually open via the connecting rod 34, allowing the powder to fall into the dissolving tank 1 at a controlled rate. The powder first falls onto the rotating dispersing frustum 22 and is guided by the arc-shaped dispersing groove 23 inside the dispersing frustum 22. The rotational force evenly disperses the powder onto different positions on the surface of the buffer solution. Simultaneously, the continuous rotation of the stirring blade 25 further promotes the mixing of the powder and the buffer solution, ensuring efficient dissolution and uniform distribution of the nuclease. After dissolution, the dissolved nuclease solution is discharged for subsequent use by controlling the valve 61 on the side of the discharge cylinder 6 at the bottom of the dissolution tank 1. The entire process not only improves the dissolution efficiency and uniformity of the nuclease, but also effectively prevents enzyme activity loss and purity reduction caused by agglomeration, thereby enhancing the subsequent application effect of the nuclease.

[0040] Based on the above description and the preferred embodiments of this utility model, it will be apparent to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0041] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A nuclease lysing device, characterized by, The utility model provides a kind of dissolving tank and its control method, including: Dissolving tank (1), top cover (11) installed on the top of the dissolving tank (1), and feed cone (12) installed on the top cover (11);The inside of the feed cone (12) is provided with opening and closing mechanism for controlling the feed rate; The inside of the dissolving tank (1) is provided with distribution stirring mechanism;The distribution stirring mechanism includes: servo motor (2) installed on the bottom of the dissolving tank (1), rotating rod (21) fixed on the output end of the servo motor (2), and dispersion round table (22) fixed on the top end of the rotating rod (21); The inside of the dispersion round table (22) is provided with a plurality of arc dispersion grooves (23), and the side surface of the rotating rod (21) is provided with a plurality of connecting rings (24), and the side surface of the connecting ring (24) is fixed with a plurality of stirring blades (25).

2. The nuclease solubilization device of claim 1, wherein: The top of the dispersion round table (22) is close to the bottom of the feed cone (12), and the two are coaxially arranged;The side surface close to the bottom end of the rotating rod (21) is rotatably connected with the bottom of the dissolving tank (1), and a plurality of the stirring blades (25) are uniformly distributed on the side surface of the connecting ring (24).

3. The nuclease solubilization device of claim 1, wherein: The opening and closing mechanism includes: movable groove (3) opened in the inside of the straight cylinder section of the feed cone (12), rotating ring (31) rotatably connected in the inside of the movable groove (3), and a plurality of hinge leaves (32) hinged in the middle of the movable groove (3);The inside of the rotating ring (31) is fixed with a plurality of connecting blocks (33), and a plurality of connecting rods (34) are hinged between a plurality of the connecting blocks (33) and a plurality of the hinge leaves (32), the side surface of the rotating ring (31) is provided with power source (35) for driving the rotating ring (31) to rotate, and limiting piece for limiting the rotation of the rotating ring (31).

4. The nuclease solubilization device of claim 3, wherein: A plurality of the hinge leaves (32) are enclosed in a circle, one side of the hinge leaf (32) is hinged with the inside of the movable groove (3), the inside of the movable groove (3) is close to the side surface of a plurality of the hinge leaves (32) and the feed cone (12) is in contact, and the movable groove (3) and the rotating ring (31) are coaxially arranged with the feed cone (12).

5. The nuclease solubilization device of claim 3, wherein: The power source (35) is one of electric cylinder, air cylinder or hydraulic cylinder;One end of the power source (35) is hinged with the inside of the movable groove (3), and the other end is hinged with the side surface of the rotating ring (31).

6. The nuclease solubilization device of claim 3, wherein: The limiting piece includes: a plurality of arc-shaped sliding grooves (4) opened on the surface of the rotating ring (31), and a plurality of convex clamping blocks (41) clamped in the inside of a plurality of the arc-shaped sliding grooves (4);One end of a plurality of the convex clamping blocks (41) is fixed with the inside of the movable groove (3).

7. The nuclease solubilization device of claim 1, wherein: The top of the top cover (11) is fixed with liquid inlet pipe (5), and the inside of the liquid inlet pipe (5) penetrates to the lower surface of the top cover (11).

8. The nuclease solubilization device of claim 1, wherein: The bottom of the dissolving tank (1) is fixed with discharge cylinder (6), the inside of the discharge cylinder (6) penetrates to the inside of the dissolving tank (1), and the side surface of the discharge cylinder (6) is provided with valve (61).

9. The nuclease solubilization device of claim 1, wherein: The side of the dissolving tank (1) is provided with an observation window (7) for observing the internal condition of the dissolving tank (1).

10. The nuclease solubilization device of claim 1, wherein: The bottom of the dissolving tank (1) is provided with a supporting frame (8) for supporting.

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