Scenedesmus obliquus carrier and pesticide wastewater biological monitoring device

Abstract

The utility model discloses a scenedesmus obliquus carrier and a pesticide wastewater biological monitoring device, which comprise a box body structure, the top of the box body structure is open, the front surface of the box body structure is provided with a carrier notch for the growth and enrichment of scenedesmus obliquus, and the carrier notch is provided with a grid plate with a three-dimensional structure; power generation fan blades are arranged at the bottom in the box body structure and are connected with a storage battery; the front face of the box body structure is further connected with a display screen, and the storage battery is electrically connected with the display screen. According to the scenedesmus obliquus carrier disclosed by the utility model, the notch for gathering the scenedesmus obliquus is reserved on the box body structure, so that a large amount of scenedesmus obliquus can be enriched and concentrated at the notch, and the scenedesmus obliquus can be ensured to have a phenomenon which is convenient for people to observe by naked eyes in a relatively fast time when heavy metal elements are contained in water, so that people can find the heavy metal elements in time; therefore, detection of heavy metals in pesticide wastewater is realized.

Description

Technical Field

[0001] This utility model relates to the field of pesticide wastewater monitoring technology, and more specifically, to a Lighthouse of the Algebraium obliquee carrier and a biological monitoring device for pesticide wastewater. Background Technology

[0002] In the field of pesticide wastewater monitoring in China, chromatography, biosensor methods, and spectrometry are the three commonly used methods. Chromatography is known for its high sensitivity and resolution, and can accurately separate and detect multiple pesticide residues, but sample pretreatment is complex and costly. Biosensor methods are characterized by rapid response, making them suitable for on-site detection and rapid screening; however, they are susceptible to electrode contamination and interfering substances. Spectrometry, especially ultraviolet-visible spectroscopy, is simple to operate and relatively inexpensive, and is often used for preliminary screening, but its sensitivity and selectivity are relatively low, and it is easily affected by sample color and turbidity.

[0003] Pesticide wastewater contains large amounts of heavy metals, among which lead and chromium are two heavy metals that have a significant impact on human activities. Lead can cumulatively poison plants and soil, affecting crop yield and quality, and impacting human health through the food chain, especially children's intellectual development and the nervous system, liver, and kidneys. Chromium, on the other hand, has toxicity determined by its form, affecting plant growth and enzyme activity. Hexavalent chromium is a potent carcinogen, and long-term exposure can damage the human nervous system, liver, and kidneys, while also polluting water bodies and disrupting the ecological balance. Therefore, effective measures must be taken to control and treat lead and chromium emissions from pesticide wastewater.

[0004] *Tetradesmus obliquus*, formerly known as *Scenedesmus obliquus*, is a common freshwater microalga belonging to the family Scenedesmusceae in the order Chlorococcalales of the phylum Chlorophyta. Its morphological characteristics include a flattened, fixed colony, typically composed of four spindle-shaped cells arranged in a straight line or slightly alternating. The cells are tapered at both ends. The colony width is 12–34 micrometers, the cell length is 10–21 micrometers, and the width is 3–9 micrometers. It is widely distributed in still water bodies such as lakes, reservoirs, ponds, and swamps, and can be found both domestically and internationally.

[0005] When faced with the stress of heavy metals lead (Pb) and chromium (Cr), *Scenedesmus obliquus* exhibits significant growth inhibition. Specifically, the presence of these heavy metals significantly inhibits the biomass accumulation of *Scenedesmus obliquus*, and the degree of inhibition gradually intensifies with increasing heavy metal concentration. This inhibition can be observed to some extent with the naked eye, manifesting as a slowed growth rate and a significant reduction in biomass. Furthermore, heavy metal stress also causes changes in the chlorophyll a content of *Scenedesmus obliquus*, specifically a decrease in chlorophyll a content, which may lead to a lighter color and yellowing of the algal cells. These changes are physiological responses of *Scenedesmus obliquus* to heavy metal stress. How to apply *Scenedesmus obliquus* to the detection of heavy metals in agricultural wastewater is a problem that needs to be solved.

[0006] In view of the above, this application is hereby submitted. Utility Model Content

[0007] To solve the above-mentioned technical problems, this utility model provides a carrier of *Scenedesmus obliquus* and a biological monitoring device for pesticide wastewater. By reserving a gap in the box structure for the aggregation of *Scenedesmus obliquus*, the *Scenedesmus obliquus* can be enriched and concentrated in large quantities at the gap. This ensures that when there are heavy metal elements in the water, *Scenedesmus obliquus* can quickly show phenomena that are easy to observe with the naked eye, so that people can detect them in time, thereby realizing the detection of heavy metals in pesticide wastewater.

[0008] This utility model is achieved through the following technical solution:

[0009] In a first aspect, this utility model provides a carrier for *Scenedesmus obliquus*, including a box structure with an opening at the top and a carrier notch for the growth and enrichment of *Scenedesmus obliquus* on the front side of the box structure. A grid plate with a three-dimensional structure is installed at the carrier notch.

[0010] The bottom of the housing structure is equipped with a generator fan blade, which is connected to a storage battery.

[0011] The front of the enclosure structure is also connected to a display screen, and the battery is electrically connected to the display screen.

[0012] The proposed carrier for *Scenedesmus obliquus* uses a pre-reserved opening in the box structure to allow for the accumulation of *Scenedesmus obliquus*. This allows the *Scenedesmus obliquus* to accumulate in large quantities at the opening, ensuring that when heavy metals are present in the water, the *Scenedesmus obliquus* can quickly exhibit a phenomenon that is easily observed by the naked eye, enabling timely detection and thus achieving the detection of heavy metals in pesticide wastewater.

[0013] The *Scenedesmus obliquus* carrier of this invention is placed in a cultivation tank containing a high density of *Scenedesmus obliquus*, with the side containing the display screen facing upwards and the generator blades aligned with the horizontal water flow direction to ensure the water flow drives the generator blades to rotate and generate electricity. The cultivation tank is placed in sunlight, allowing the *Scenedesmus obliquus* to accumulate and grow on the three-dimensional mesh plate at the carrier's notch. Once the mesh plate at the carrier's notch is completely covered by *Scenedesmus obliquus*, the carrier is removed, and it can then be used as a device for detecting heavy metals in agricultural wastewater. The carrier loaded with *Scenedesmus obliquus* is placed in the pesticide wastewater to be tested and left for one day to observe changes in the color and morphology of the *Scenedesmus obliquus*. If fading, significant changes in shape, or large-scale detachment of the *Scenedesmus obliquus* from the notch occurs, it indicates that the wastewater contains a high level of heavy metals.

[0014] Furthermore, because *Scenedesmus obliquus* is highly adaptable and has low environmental requirements, if no changes are observed in the wastewater within a short period, it can be cultured and observed for an extended period to monitor the heavy metal content in the water.

[0015] This invention features a three-dimensional mesh plate with a porous structure installed at the gap in the carrier. This structure ensures that *Scenedesmus obliquus* can accumulate and grow in the gap. The three-dimensional mesh plate is also permeable, allowing water in the cultivation tank to pass through and provide ample water for the *Scenedesmus obliquus*. Furthermore, the three-dimensional mesh plate helps to restrain the *Scenedesmus obliquus*, preventing it from being dispersed by water flow and ensuring maximum accumulation at the carrier gap for observation during subsequent agricultural wastewater testing.

[0016] In one specific embodiment, a thermometer and a pH meter are also installed inside the enclosure structure. The thermometer and pH meter are powered by a battery and connected to the display screen.

[0017] This invention also includes a generator fan blade inside the box structure. When the carrier is placed in the culture tank and loaded with Scenedesmus obliquus, the flow of water in the culture tank can drive the generator fan blade to rotate and generate electricity. As a generator, a display screen, thermometer and pH meter are also installed, so that the temperature and pH value of the water in the culture tank can be displayed and observed at any time, thereby ensuring a good growth environment for Scenedesmus obliquus loaded on the carrier.

[0018] In one specific embodiment, the two side walls of the box structure are provided with protruding structures and recessed structures, and the protruding structures and recessed structures are matched in shape and size so that they can be interlocked with each other.

[0019] This invention features interlocking protrusions and recesses on both sides of the box structure, facilitating the assembly of multiple box structures. When the water tank area to be tested is large, multiple Scenedesmus obliquus carriers can be assembled to monitor more water, preventing heavy metal elements in wastewater from spreading on a small scale and being difficult to detect. More carriers carrying more Scenedesmus obliquus also result in higher accuracy when testing for heavy metal elements in the water tank.

[0020] In one specific embodiment, the three-dimensional mesh plate is provided with multiple layers, so that with a fixed carrier gap area, more oblique Scenedesmus can be loaded through the multi-layer mesh plate.

[0021] In one specific embodiment, the grid plate is a biofilm plate, which can promote the growth of algae on the grid plate, thereby ensuring the enrichment of *Scenedesmus obliquus* and preventing the concentration of *Scenedesmus obliquus* from decreasing due to water flow. The specific selection of the biofilm plate can be based on commonly available biofilms on the market, and this application does not limit its selection.

[0022] In one specific embodiment, the protruding structure protrudes from the side wall of the box structure. The protruding structure includes a protruding post, and the end of the protruding post away from the box structure has a locking cylinder. The diameter of the locking cylinder is greater than the width of the protruding post.

[0023] In this invention, when two box structures need to be assembled, the protruding structure on one side of one box structure is inserted from top to bottom into the recessed structure on one side of the other box structure until the two box structures are side by side and at the same height. At this time, due to the limiting of the locking cylinder, the two box structures will not separate when subjected to horizontal tension, thus playing a role in fixing each other and ensuring the accuracy of water sample monitoring.

[0024] In one specific implementation, the box structure is made of polyurethane polymer material, which has good hardness and facilitates long-term use of the box structure.

[0025] In one specific implementation, the grid plate is detachably connected to the carrier gap of the box structure.

[0026] Secondly, this utility model provides a biological monitoring device for pesticide wastewater, including the oblique-grown Scenedesmus carrier, wherein the oblique-grown Scenedesmus carrier has a three-dimensional grid plate covered with growing Scenedesmus.

[0027] In one specific embodiment, the pesticide wastewater biological monitoring device includes several Scenedesmus obliquus carriers, and adjacent Scenedesmus obliquus carriers are assembled into one unit through protruding and concave structures on both sides.

[0028] Compared with the prior art, this utility model has the following advantages and beneficial effects:

[0029] 1. The present invention provides a carrier of *Scenedesmus obliquus* and a biological monitoring device for pesticide wastewater. By reserving a gap in the box structure for the aggregation of *Scenedesmus obliquus*, the *Scenedesmus obliquus* can be enriched and concentrated in large quantities at the gap. This ensures that when there are heavy metal elements in the water, *Scenedesmus obliquus* can quickly show a phenomenon that is easy to observe with the naked eye, so that people can detect it in time, thereby realizing the detection of heavy metals in pesticide wastewater.

[0030] 2. The present invention provides a carrier of *Scenedesmus obliquus* and a biological monitoring device for pesticide wastewater. A three-dimensional grid plate is installed at the gap of the carrier. The grid plate has a three-dimensional porous structure, which can ensure that *Scenedesmus obliquus* can accumulate and grow in the gap. At the same time, the three-dimensional grid plate is also permeable, which can ensure that the water in the cultivation tank can penetrate the grid plate and provide sufficient water for *Scenedesmus obliquus*. The three-dimensional grid plate can also bind *Scenedesmus obliquus*, ensuring that *Scenedesmus obliquus* will not be dispersed by the water flow in the cultivation tank, and ensuring that *Scenedesmus obliquus* accumulates to the maximum extent at the gap of the carrier, so as to facilitate the observation of phenomena during subsequent agricultural wastewater testing.

[0031] 3. The present invention provides a carrier of *Scenedesmus obliquus* and a biological monitoring device for pesticide wastewater. The box structure is also equipped with a generator fan blade. When the carrier is placed in the culture tank and loaded with *Scenedesmus obliquus*, the water flow in the culture tank can be used to drive the generator fan blade to rotate and generate electricity. As a generator, a display screen, thermometer and pH meter are also installed. This allows the temperature and pH value of the water in the culture tank to be displayed and observed at any time, thereby ensuring a good growth environment for *Scenedesmus obliquus* loaded on the carrier.

[0032] 4. The proposed embodiment of this utility model provides a *Scenedesmus obliquus* carrier and a biological monitoring device for pesticide wastewater. By providing interlocking protrusions and recesses on both sides of the box structure, multiple box structures can be easily assembled and spliced. When the area of ​​the water pool to be tested is large, multiple *Scenedesmus obliquus* carriers can be assembled to monitor more water, avoiding the small-scale diffusion of heavy metal elements in the wastewater, which makes them difficult to detect. More carriers carrying more *Scenedesmus obliquus* can also achieve higher accuracy when testing for heavy metal elements in the water pool. Attached Figure Description

[0033] To more clearly illustrate the technical solutions of the exemplary embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0034] Figure 1 A schematic diagram of the structure of the Scenedesmus obliquus carrier provided in this embodiment of the present invention;

[0035] Figure 2 A front view of the oblique-growing Scenedesmus carrier provided in an embodiment of this utility model;

[0036] Figure 3 A schematic diagram of the splicing structure of the Scenedesmus obliqueis carrier provided in an embodiment of this utility model.

[0037] The attached diagram shows the markings and corresponding component names:

[0038] 1-Box structure, 2-Carrier notch, 3-Power generator blade, 4-Display screen, 5-Protruding structure, 6-Recessed structure. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the embodiments and accompanying drawings. The illustrative embodiments and descriptions of this utility model are only used to explain this utility model and are not intended to limit this utility model.

[0040] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, it will be apparent to those skilled in the art that these specific details are not necessary to implement the present invention. In other embodiments, well-known structures are not specifically described in order to avoid obscuring the present invention.

[0041] Throughout this specification, references to "an embodiment," "an example," or "an example" mean that a particular feature, structure, or characteristic described in connection with that embodiment or example is included in at least one embodiment of the present invention. Therefore, the phrases "an embodiment," "an example," "an example," or "an example" appearing in various places throughout the specification do not necessarily refer to the same embodiment or example. Furthermore, specific features, structures, or characteristics can be combined in one or more embodiments or examples in any suitable combination and / or sub-combination. Moreover, those skilled in the art will understand that the illustrations provided herein are for illustrative purposes and are not necessarily drawn to scale. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0042] In the description of this utility model, the terms "front", "rear", "left", "right", "up", "down", "vertical", "horizontal", "high", "low", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this utility model.

[0043] Example

[0044] like Figures 1-3 As shown in the figure, the present invention provides a carrier of *Scenedesmus obliquus* and a biological monitoring device for pesticide wastewater, including a box structure 1, an opening at the top of the box structure 1, a carrier notch 2 for the growth and enrichment of *Scenedesmus obliquus* on the front of the box structure 1, and a grid plate with a three-dimensional structure installed at the carrier notch 2.

[0045] The bottom of the housing structure 1 is provided with a generator fan blade 3, and the generator fan blade 3 is connected to a storage battery;

[0046] The front of the housing structure 1 is also connected to a display screen 4, and the battery is electrically connected to the display screen 4.

[0047] The proposed carrier for *Scenedesmus obliquus* uses a pre-reserved opening in the box structure to allow for the accumulation of *Scenedesmus obliquus*. This allows the *Scenedesmus obliquus* to accumulate in large quantities at the opening, ensuring that when heavy metals are present in the water, the *Scenedesmus obliquus* can quickly exhibit a phenomenon that is easily observed by the naked eye, enabling timely detection and thus achieving the detection of heavy metals in pesticide wastewater.

[0048] The *Scenedesmus obliquus* carrier of this invention is placed in a cultivation tank containing a high density of *Scenedesmus obliquus*, with the side containing the display screen facing upwards and the generator blades aligned with the horizontal water flow direction to ensure the water flow drives the generator blades to rotate and generate electricity. The cultivation tank is placed in sunlight, allowing the *Scenedesmus obliquus* to accumulate and grow on the three-dimensional mesh plate at the carrier's notch. Once the mesh plate at the carrier's notch is completely covered by *Scenedesmus obliquus*, the carrier is removed, and it can then be used as a device for detecting heavy metals in agricultural wastewater. The carrier loaded with *Scenedesmus obliquus* is placed in the pesticide wastewater to be tested and left for one day to observe changes in the color and morphology of the *Scenedesmus obliquus*. If fading, significant changes in shape, or large-scale detachment of the *Scenedesmus obliquus* from the notch occurs, it indicates that the wastewater contains a high level of heavy metals.

[0049] Furthermore, because *Scenedesmus obliquus* is highly adaptable and has low environmental requirements, if no changes are observed in the wastewater within a short period, it can be cultured and observed for an extended period to monitor the heavy metal content in the water.

[0050] This invention features a three-dimensional mesh plate with a porous structure installed at the gap in the carrier. This structure ensures that *Scenedesmus obliquus* can accumulate and grow in the gap. The three-dimensional mesh plate is also permeable, allowing water in the cultivation tank to pass through and provide ample water for the *Scenedesmus obliquus*. Furthermore, the three-dimensional mesh plate helps to restrain the *Scenedesmus obliquus*, preventing it from being dispersed by water flow and ensuring maximum accumulation at the carrier gap for observation during subsequent agricultural wastewater testing.

[0051] In one specific embodiment, a thermometer and a pH meter are also installed inside the housing structure 1. The thermometer and pH meter are powered by a storage battery and connected to the display screen 4.

[0052] This invention also includes a generator fan blade inside the box structure. When the carrier is placed in the culture tank and loaded with Scenedesmus obliquus, the flow of water in the culture tank can drive the generator fan blade to rotate and generate electricity. As a generator, a display screen, thermometer and pH meter are also installed, so that the temperature and pH value of the water in the culture tank can be displayed and observed at any time, thereby ensuring a good growth environment for Scenedesmus obliquus loaded on the carrier.

[0053] In one specific embodiment, the two side walls of the box structure 1 are provided with protruding structures 5 and recessed structures 6, and the protruding structures 5 and recessed structures 6 are matched in shape and size and can be interlocked with each other.

[0054] This invention features interlocking protrusions and recesses on both sides of the box structure, facilitating the assembly of multiple box structures. When the water tank area to be tested is large, multiple Scenedesmus obliquus carriers can be assembled to monitor more water, preventing heavy metal elements in wastewater from spreading on a small scale and being difficult to detect. More carriers carrying more Scenedesmus obliquus also result in higher accuracy when testing for heavy metal elements in the water tank.

[0055] In one specific embodiment, the three-dimensional mesh plate is provided with multiple layers, so that with a fixed carrier gap area, more oblique Scenedesmus can be loaded through the multi-layer mesh plate.

[0056] In one specific embodiment, the grid plate is a biofilm plate, which can promote the growth of algae on the grid plate, thereby ensuring the enrichment of *Scenedesmus obliquus* and preventing the concentration of *Scenedesmus obliquus* from decreasing due to water flow. The specific selection of the biofilm plate can be based on commonly available biofilms on the market, and this application does not limit its selection.

[0057] In a specific embodiment, the protruding structure 5 protrudes from the side wall of the box structure 1. The protruding structure 5 includes a protruding post, and the end of the protruding post away from the box structure 1 has a locking cylinder. The diameter of the locking cylinder is greater than the width of the protruding post.

[0058] In this invention, when two box structures need to be assembled, the protruding structure on one side of one box structure is inserted from top to bottom into the recessed structure on one side of the other box structure until the two box structures are side by side and at the same height. At this time, due to the limiting of the locking cylinder, the two box structures will not separate when subjected to horizontal tension, thus playing a role in fixing each other and ensuring the accuracy of water sample monitoring.

[0059] In one specific implementation, the box structure 1 is made of polyurethane polymer material, which has good hardness and facilitates long-term use of the box structure.

[0060] In a specific implementation, the grid plate is detachably connected to the carrier notch 2 of the box structure 1.

[0061] Secondly, this utility model provides a biological monitoring device for pesticide wastewater, including the oblique-grown Scenedesmus carrier, wherein the oblique-grown Scenedesmus carrier has a three-dimensional grid plate covered with growing Scenedesmus.

[0062] In one specific embodiment, the pesticide wastewater biological monitoring device includes several Scenedesmus obliquus carriers, and adjacent Scenedesmus obliquus carriers are assembled into one unit through the protruding structure 5 and the concave structure 6 on both sides.

[0063] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of this utility model. It should be understood that the above description is only a specific embodiment of this utility model and is not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the scope of protection of this utility model.

Claims

1. A carrier of Oedogonium fimbriatum, characterized in that, The application relates to a box structure (1) which is provided with an open top, a carrier notch (2) for growth and enrichment of cladophora on the front surface of the box structure (1), and a grid plate with a three-dimensional structure is arranged at the carrier notch (2). An electricity generating fan blade (3) is arranged at the bottom of the box structure (1), and the electricity generating fan blade (3) is connected with a storage battery. A display screen (4) is further connected to the front surface of the box structure (1), and the storage battery is electrically connected with the display screen (4).

2. The Cladophora carrier of claim 1, wherein, Protruding structures (5) and recessed structures (6) are arranged on the two side walls of the box structure (1), and the protruding structures (5) and the recessed structures (6) are matched in shape and size and can be clamped with each other.

3. The Cladophora carrier of claim 1, wherein, The grid plate with the three-dimensional structure is provided with multiple layers.

4. The Cladophora carrier of claim 3, wherein, The grid plate is a biofilm plate.

5. The Cladophora carrier of claim 1, wherein, A thermometer and a pH meter are further arranged in the box structure (1), the thermometer and the pH meter are powered by the storage battery and are connected with the display screen (4).

6. The Cladophora carrier of claim 2, wherein, The protruding structure (5) protrudes from the side wall of the box structure (1), the protruding structure (5) comprises a protruding column, one end of the protruding column away from the box structure (1) is provided with a clamping cylinder, and the diameter of the clamping cylinder is larger than the width of the protruding column.

7. The Scenedesrnus obliquus carrier of claim 1, wherein, The box structure (1) is made of polyurethane polymer material.

8. The onoxenium carrier of claim 1, wherein, The grid plate is detachably connected with the carrier notch (2) of the box structure (1).

9. A pesticide wastewater biological monitoring device, characterized by, The application further relates to a cladophora carrier, and cladophora is grown on the grid plate with the three-dimensional structure of the cladophora carrier.

10. A device for biological monitoring of pesticide waste water according to claim 9, characterized in that A plurality of the cladophora carriers are assembled into one body through the protruding structures (5) and the recessed structures (6) on the two sides.

Images