Nucleic acid purification equipment easy to operate

Abstract

The utility model discloses nucleic acid purification equipment easy to operate, which belongs to the technical field of nucleic acid extraction and comprises a base, a posture adjusting device, a plurality of uniform mixing devices and an auxiliary cleaning device, the posture adjusting device is mounted on the right side of the base, the uniform mixing devices are mounted on the upper side of the posture adjusting device, and the auxiliary cleaning device is mounted on the base. The auxiliary cleaning device is installed on the upper side of the base, the uniform mixing device comprises a shell, sliding supports, a moving frame, a spring, a supporting pipe, a first electromagnet and a permanent magnet, the shell is fixedly installed on the posture adjusting device, and the two sliding supports are slidably connected to the left side and the right side of the shell in a bilateral symmetry mode along the center axis of the shell; the moving frame is fixedly installed between the two sliding supports. By means of the mode, magnetic beads can be synchronously and evenly distributed on multiple sets of samples at the same time, the inclination angle can be rapidly adjusted, and rapid airing and sterilization after cleaning can be assisted.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid extraction technical field, specifically easy to operate's nucleic acid purification equipment. BACKGROUND

[0002] Nucleic acid purification is a key technology in molecular biology experiments, aiming at separating high-purity nucleic acids such as DNA or RNA from complex biological samples. In addition to the target nucleic acid, biological samples also contain impurities such as proteins, polysaccharides, and lipids. These impurities may interfere with subsequent experiments such as enzyme digestion, PCR, and sequencing. Nucleic acid purification can remove these impurities and improve the purity of nucleic acids. One method of nucleic acid purification is magnetic bead method, which is simple to operate and has high extraction efficiency. Its principle is to use specific groups on the surface of magnetic beads to specifically bind to nucleic acids. Under the action of a magnetic field, magnetic beads adsorbed with nucleic acids can be quickly separated from other impurities in the sample. Then, the impurities are removed by washing, and finally the nucleic acids are eluted from the magnetic beads with eluent. The magnetic beads of nucleic acids are also called superparamagnetic nanometer microspheres. Its interior is a magnetic core, so under the action of an external magnetic field, the microspheres can move directionally. The outside is a coating layer, and the surface is distributed with many active groups that can couple with biochemical reagents such as cells, proteins, nucleic acids, and enzymes, and then realize separation under the action of a magnetic field.

[0003] The Chinese patent with publication number CN215828744U proposes a nucleic acid extraction and purification equipment, which includes a sample tube placement table and a base. The sample tube placement table is provided with a sample tube slot, and the base is provided with a mixing device. The mixing device includes a rotating magnet and a driving device. The driving device is used to drive the rotating magnet to rotate around the sample tube slot. The base is also provided with a controller, and the base is provided with a power connector. The controller, power connector, and driving device are electrically connected. The power connector is used to connect a direct current power supply. The driving device includes a magnet fixing seat, a magnet shaft, a first gear, a second gear, and a motor. The rotating magnet, magnet fixing seat, magnet shaft, and first gear are sequentially fixedly connected. The first gear is coaxial with the magnet shaft. The second gear is engaged with the first gear. The second gear is power connected with the motor. The rotating magnet is an electromagnet. The magnet shaft surface is provided with a first electrode and a second electrode.

[0004] However, the technical solution of this patent has the following problems:

[0005] This patent cannot simultaneously distribute magnetic beads uniformly to multiple groups of samples while oscillating, and cannot assist in air drying and sterilization after cleaning.

[0006] Therefore, the skilled person in the art provides a nucleic acid purification equipment that is easy to operate to solve the above problems. UTILITY MODEL CONTENT

[0007] The utility model discloses a nucleic acid purification equipment easy to operate to solve the problem of the above background technology.

[0008] To achieve the above object, the utility model provides the following technical scheme:

[0009] A nucleic acid purification equipment easy to operate, including base, still include: attitude adjusting device, uniform mixing device and auxiliary cleaning device, the attitude adjusting device installs at the right side of base, a plurality of uniform mixing devices are installed on the upside of attitude adjusting device, the auxiliary cleaning device is installed on the upside of base, the uniform mixing device includes: shell, sliding support, moving frame, spring, support pipe, first electromagnet and permanent magnet, the shell is fixedly installed on the attitude adjusting device, two sliding supports are symmetrically slidably connected on the left and right sides of the shell, the moving frame is fixedly installed between two sliding supports, a plurality of springs are arranged on the sliding support, one end of the spring is close to the shell, the other end of the spring is close to the moving frame, a plurality of support pipes are fixedly installed on the moving frame at equal intervals, every support pipe downside is provided with a first electromagnet, the first electromagnet is fixedly installed on the downside of moving frame, two permanent magnets are fixedly installed on the downside of shell, and the permanent magnet is located between two adjacent first electromagnets.

[0010] Further, the auxiliary mixing device is installed on the upside of the shell.

[0011] Further, the auxiliary mixing device includes: rotating plate and support plate, the rotating plate is hinged on the left upside of the shell, the support plate is fixedly installed on the right upside of the shell, and the rotating plate right side is clamped on the upside of the support plate.

[0012] Further, the auxiliary mixing device further includes: second electromagnet, a plurality of second electromagnets are fixedly installed on the downside of the rotating plate, and the second electromagnet is located directly above the support pipe.

[0013] Further, the attitude adjusting device includes: connecting plate and worm gear, the connecting plate is fixedly installed on the left and right two side walls of the shell, one end of the connecting plate away from the shell is rotatably connected on the base through the pivot, and the worm gear is fixedly installed on the pivot of the right connecting plate.

[0014] Further, the attitude adjusting device further includes: worm and knob, the worm is rotatably connected on the right side of the base through the pivot, the worm and the worm gear are meshed with each other, and the knob is fixedly installed on the pivot of the worm.

[0015] Further, the base middle side is provided with a slot.

[0016] Further, the auxiliary cleaning device comprises a transparent plate; the transparent plate is fixedly installed in the groove;

[0017] Further, the auxiliary cleaning device further comprises: a plurality of ultraviolet lamp tubes, the plurality of ultraviolet lamp tubes are fixedly installed in the groove;

[0018] Further, the auxiliary cleaning device further comprises: a plurality of infrared lamp tubes, the plurality of infrared lamp tubes are fixedly installed in the groove, and the first electromagnet, the second electromagnet, the ultraviolet lamp tubes and the infrared lamp tubes are controlled by an external controller.

[0019] Compared with the prior art, the auxiliary cleaning device has the beneficial effects that: 1. The PCR eight-tube is placed in the support tube, the appropriate magnetic beads and buffer solution are injected into the PCR eight-tube, and the sample is added, the first electromagnet of the uniform mixing device is controlled by the external controller, the adjacent first electromagnets are not started at the same time, the intermittent magnetic field is generated by the intermittent start of the first electromagnet, the magnetic field and the magnetic field of the permanent magnet interact, the moving frame moves left and right, the spring is elastically deformed to assist the moving frame to return to the center, and the intermittent magnetic field generated by the first electromagnet can attract or repel the magnetic beads, so that the movement of the magnetic beads in the sample is accelerated, and the magnetic beads are uniformly distributed in the sample, which is beneficial to the synchronous and uniform distribution of the magnetic beads in the multiple groups of samples at the same time.

[0020] 2. The PCR eight-tube on the upper side of the support tube is covered by rotating the cover plate, the second electromagnet is intermittently started and stopped by the external controller, the intermittent magnetic field is generated on the upper side of the PCR eight-tube, the magnetic beads are intermittently attracted by the magnetic field, the magnetic beads are intermittently attracted downward or upward by the magnetic field generated by the first electromagnet, the magnetic beads move up and down in the sample, which is beneficial to the rapid adsorption of the magnetic beads to the nucleic acid in the sample; the knob is rotated to drive the worm to rotate, the worm is rotated to drive the worm wheel to rotate, the worm wheel is rotated to drive the connecting plate to rotate by a certain angle, the connecting plate is rotated by a certain angle to drive the shell to rotate by a certain angle, so that the operator can quickly adjust the inclination angle of the shell, and it is easier to perform liquid suction and other operations; the support tube is rotated by one hundred and eighty degrees, the ultraviolet lamp tube is turned on to quickly sterilize the inner wall of the support tube; the infrared lamp tube is turned on to quickly dry the residual water droplets on the inner wall of the support tube. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is a three-dimensional structure diagram of the utility model Figure 1 ;

[0022] Figure 2 It is a front view of the utility model;

[0023] Figure 3 It is a three-dimensional structure diagram of the utility model Figure 2 ;

[0024] Figure 4 It is the three-dimensional structure schematic view of the utility model removes rotating plate and transparent plate;

[0025] Figure 5 It is the three-dimensional section view of the utility model's integral part structure;

[0026] Figure 6 It is the structure schematic view of the utility model's uniform mixing device.

[0027] In the drawing: 1, base; 2, attitude adjusting device; 21, connecting plate; 22, worm gear; 23, worm; 24, knob; 3, uniform mixing device; 31, shell; 32, sliding support; 33, moving frame; 34, spring; 35, support tube; 36, first electromagnet; 37, permanent magnet; 38, rotating plate; 39, support plate; 310, second electromagnet; 4, auxiliary cleaning device; 41, transparent plate; 42, ultraviolet lamp tube; 43, infrared lamp tube; 5, grooving. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0029] In the following description, "left", "right", "front", "back", "up", "down" are oriented with the perspective of the front view.

[0030] Embodiment one: in some embodiments, please refer to the accompanying drawings of the specification Figures 1-6The utility model provides a nucleic acid purification equipment easy to operate, including base 1, still include: posture adjusting device 2, uniform mixing device 3 and auxiliary cleaning device 4, posture adjusting device 2 installs on the right side of base 1, a plurality of uniform mixing device 3 installs on the upper side of posture adjusting device 2, auxiliary cleaning device 4 installs on the upper side of base 1, uniform mixing device 3 includes: shell 31, sliding support 32, moving frame 33, spring 34, support tube 35, first electromagnet 36 and permanent magnet 37, shell 31 is fixedly installed on posture adjusting device 2, two sliding supports 32 are symmetrically slidably connected on the left and right sides of shell 31, moving frame 33 is fixedly installed between two sliding supports 32, a plurality of springs 34 are arranged on sliding support 32, one end of spring 34 is close to shell 31, the other end of spring 34 is close to moving frame 33, a plurality of support tubes 35 are fixedly installed on moving frame 33 at equal intervals, every support tube 35 is provided with a first electromagnet 36 on the lower side, and the first electromagnet 36 is fixedly installed on the lower side of moving frame 33, two permanent magnets 37 are fixedly installed on the lower side of shell 31, and the permanent magnets 37 are located between two adjacent first electromagnets 36.

[0031] The posture adjusting device 2 can quickly adjust the posture, so that the equipment can be inclined at a certain angle, facilitating the operator to perform liquid suction and other operations, the uniform mixing device 3 can quickly and uniformly mix the magnetic beads and the sample, and the auxiliary cleaning device 4 can assist in drying and sterilizing the equipment after cleaning.

[0032] The PCR eight-tube is placed in the support tube 35, an appropriate amount of magnetic beads and buffer solution are injected into the PCR eight-tube, and then the sample is added, the first electromagnet 36 of the uniform mixing device 3 is controlled by an external controller, adjacent first electromagnets 36 are not started at the same time, the first electromagnet 36 is intermittently started to generate an intermittent magnetic field, the magnetic field and the magnetic field of the permanent magnet 37 interact to make the moving frame 33 move left and right, the spring 34 is elastically deformed to assist the moving frame 33 to return to the center, and the intermittent magnetic field generated by the first electromagnet 36 can attract or repel the magnetic beads, accelerate the movement of the magnetic beads in the sample, and make the magnetic beads be uniformly distributed in the sample, which is beneficial to simultaneously and uniformly distribute the magnetic beads in multiple groups of samples.

[0033] The shell 31 is provided with an auxiliary mixing device on the upper side.

[0034] The auxiliary mixing device comprises a rotating plate 38 and a supporting plate 39, the rotating plate 38 is hinged to the upper left side of the shell 31, the supporting plate 39 is fixedly installed on the upper right side of the shell 31, and the right side of the rotating plate 38 is clamped on the upper side of the supporting plate 39.

[0035] The auxiliary mixing device further comprises a plurality of second electromagnets 310 fixedly installed on the lower side of the rotating plate 38, and the second electromagnets 310 are located directly above the supporting tube 35.

[0036] The rotating plate 38 covers the upper side of the PCR octuple tube of the supporting tube 35, and the external controller controls the intermittent starting and stopping of the second electromagnets 310, so that the upper side of the PCR octuple tube generates an intermittent magnetic field, which intermittently attracts the magnetic beads, and cooperates with the magnetic field generated by the first electromagnet 36 to intermittently attract the magnetic beads downward or upward, so that the magnetic beads move up and down in the sample, which is beneficial to the rapid adsorption of the magnetic beads to the nucleic acid in the sample.

[0037] The posture adjusting device 2 comprises a connecting plate 21 and a worm gear 22, the connecting plate 21 is fixedly installed on the left and right side walls of the shell 31, one end of the connecting plate 21 away from the shell 31 is rotationally connected to the base 1 through a rotating shaft, and the worm gear 22 is fixedly installed on the rotating shaft of the right connecting plate 21.

[0038] The posture adjusting device 2 further comprises a worm 23 and a knob 24, the worm 23 is rotationally connected to the right side of the base 1 through a rotating shaft, the worm 23 and the worm gear 22 are meshed with each other, and the knob 24 is fixedly installed on the rotating shaft of the worm 23.

[0039] The rotation of the knob 24 drives the rotation of the worm 23, the rotation of the worm 23 drives the rotation of the worm gear 22, the rotation of the worm gear 22 drives the rotation of the connecting plate 21 by a certain angle, and the rotation of the connecting plate 21 by a certain angle drives the rotation of the shell 31 by a certain angle, so that the operator can quickly adjust the inclination angle of the shell 31, and it is easier to perform liquid suction and other operations.

[0040] A slot 5 is formed in the middle of the base 1.

[0041] The auxiliary cleaning device 4 comprises a transparent plate 41, and the transparent plate 41 is fixedly installed in the slot 5 and used for isolating water droplets remaining in the PCR octuple tube or the supporting tube 35 due to cleaning.

[0042] The auxiliary cleaning device 4 further comprises a plurality of ultraviolet lamp tubes 42 fixedly installed in the slot 5, the supporting tube 35 is rotated by one hundred and eighty degrees, and the ultraviolet lamp tubes 42 are turned on to quickly sterilize the inner wall of the supporting tube 35.

[0043] The auxiliary cleaning device 4 further comprises a plurality of infrared lamp tubes 43 fixedly installed in the slot 5, and the first electromagnet 36, the second electromagnet 310, the ultraviolet lamp tubes 42 and the infrared lamp tubes 43 are all controlled by an external controller. The supporting tube 35 is rotated by one hundred and eighty degrees, and the infrared lamp tubes 43 are turned on to quickly dry the water droplets remaining on the inner wall of the supporting tube 35.

[0044] The above embodiments are only used to illustrate the technical solutions of the present application, and are not intended to limit the present application; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features therein can be replaced by equivalents; and these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. An easy-to-operate nucleic acid purification apparatus comprising a base (1), characterized in that, Also include: The posture adjusting device (2), the uniform mixing device (3) and the auxiliary cleaning device (4), the posture adjusting device (2) is installed on the right side of the base (1), a plurality of the uniform mixing device (3) is installed on the upper side of the posture adjusting device (2), the auxiliary cleaning device (4) is installed on the upper side of the base (1), the uniform mixing device (3) includes: shell (31), sliding bracket (32), moving frame (33), spring (34), support tube (35), first electromagnet (36) and permanent magnet (37), the shell (31) is fixedly installed on the posture adjusting device (2), two the sliding bracket (32) is symmetrically slidably connected on the left and right sides of the shell (31), the moving frame (33) is fixedly installed between the two sliding brackets (32), a plurality of the spring (34) is arranged on the sliding bracket (32), one end of the spring (34) is close to the shell (31), the other end of the spring (34) is close to the moving frame (33), a plurality of the support tube (35) is fixedly installed on the moving frame (33) at equal intervals, each support tube (35) is provided with a first electromagnet (36) on the lower side, the first electromagnet (36) is fixedly installed on the lower side of the moving frame (33), two the permanent magnet (37) is fixedly installed on the lower side of the shell (31), and the permanent magnet (37) is located between two adjacent first electromagnets (36).

2. The easy-to-operate nucleic acid purification apparatus according to claim 1, characterized by, The upper side of the shell (31) is provided with an auxiliary mixing device.

3. The easy-to-operate nucleic acid purification apparatus according to claim 2, characterized by, The auxiliary mixing device includes: rotating plate (38) and support plate (39), the rotating plate (38) is hinged on the upper left side of the shell (31), and the support plate (39) is fixedly installed on the upper right side of the shell (31). The right side of the rotating plate (38) is clamped on the upper side of the support plate (39).

4. The easy-to-operate nucleic acid purification apparatus according to claim 3, characterized by The auxiliary mixing device further includes: a plurality of second electromagnets (310) fixedly installed on the lower side of the rotating plate (38).

5. The easy-to-operate nucleic acid purification apparatus according to claim 4, characterized by The second electromagnet (310) is located directly above the support tube (35).

6. The easy-to-operate nucleic acid purification apparatus according to claim 1, wherein The posture adjusting device (2) includes: connecting plate (21) and worm gear (22), the connecting plate (21) is fixedly installed on the left and right side walls of the shell (31), and the end, away from the shell (31), of the connecting plate (21) is rotatably connected to the base (1) through a rotating shaft, and the worm gear (22) is fixedly installed on the rotating shaft of the right connecting plate (21).

7. The easy-to-operate nucleic acid purification device according to claim 6, characterized in that The posture adjusting device (2) further includes: a worm (23) and a knob (24), the worm (23) is rotatably connected to the right side of the base (1) through a rotating shaft, the worm (23) and the worm gear (22) are meshed with each other, and the knob (24) is fixedly installed on the rotating shaft of the worm (23).

8. The easy-to-operate nucleic acid purification apparatus according to claim 1, characterized by, The middle side of the base (1) is provided with a slot (5).

9. The easy-to-operate nucleic acid purification device according to claim 8, characterized in that, The auxiliary cleaning device (4) includes: a transparent plate (41); the transparent plate (41) is fixedly installed in the slot (5).

10. The easy-to-operate nucleic acid purification apparatus according to claim 8, characterized by The auxiliary cleaning device (4) further comprises: ultraviolet lamp tubes (42), a plurality of the ultraviolet lamp tubes (42) are fixedly installed in the slot (5), and the auxiliary cleaning device (4) further comprises: infrared lamp tubes (43), a plurality of the infrared lamp tubes (43) are fixedly installed in the slot (5).

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