Safe transition non-contact pipetting device

Abstract

The utility model belongs to the technical field of liquid movement, and particularly relates to a safe transition non-contact pipetting device which comprises a pipette with liquid level scale marks, a suction rubber head, a pipette joint, a pressure relief joint, a one-way air valve and a pressure relief structure. The squeezed suction rubber head does not need to be inserted into the transfer pipette in alignment with the transfer pipette, so that the complexity during working is reduced, and the situation that part of liquid leaks from the bottom of the transfer pipette before a finger covers the top of the transfer pipette or when the finger moves away from the top of the transfer pipette when the suction rubber head is taken and placed each time is avoided; and when the suction rubber head is placed, the suction rubber head cannot be accurately inserted into the top of the transfer pipette at a time, and if the suction rubber head is not placed at a time, most liquid leaks from the bottom of the transfer pipette.

Description

Technical Field

[0001] This utility model belongs to the field of liquid transfer technology, and in particular relates to a safe, contactless transfer device. Background Technology

[0002] In pharmaceutical preparation, different reagents need to be mixed and reacted in different proportions. Liquids are drawn from the container and transferred to the appropriate reaction vessel using a pipette. The traditional method involves a separate pipette and suction tip. The suction tip is inserted into the top of the pipette, and squeezing it expels the air. Releasing the tip creates negative pressure, drawing the liquid into the pipette. However, during use, the suction tip must be removed from the top of the pipette each time it is squeezed, and a finger must be placed over the top of the pipette to prevent leakage from the bottom. However, some liquid will leak from the bottom of the pipette before the finger is placed over it.

[0003] Secondly, when it's necessary to reinsert the aspiration tip into the top of the pipette, the finger must be removed and the aspiration tip aligned with the top of the pipette before insertion. This process cannot be completed in one smooth motion to accurately insert the aspiration tip into the top of the pipette. If it's not inserted correctly in one go, a large amount of liquid will leak out from the bottom of the pipette, greatly affecting the efficiency of liquid extraction. Moreover, during the aspiration process, liquid is often drawn up to above the target level, and then drained to gradually bring it level with the target level. The traditional draining method is to release the finger from the top of the pipette to let the liquid flow down, but it's difficult for operators to control the timing and force of releasing the finger, which can easily lead to over-draining, causing the liquid to be below the target level. In this case, the aspiration operation needs to be repeated, increasing the operation time and workload while reducing overall work efficiency. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] This invention provides a safe, contactless pipetting device to solve the following problems.

[0006] 1. Each time the aspiration tip is picked up or placed, some liquid leaks from the bottom of the pipette before the finger covers the top of the pipette or when the finger is removed from the top of the pipette. Furthermore, it is impossible to insert the aspiration tip accurately into the top of the pipette in one smooth motion. If it is not placed correctly in one go, most of the liquid will leak from the bottom of the pipette, which greatly affects the efficiency of liquid extraction.

[0007] 2. The traditional method of draining liquid involves releasing the finger from the top of the pipette to allow the liquid to flow down. However, it is difficult for staff to control the timing and force of releasing the finger, which can easily lead to over-draining. As a result, the liquid may be below the target level, requiring another suction operation, which reduces the overall work efficiency.

[0008] (II) Technical Content

[0009] To achieve the above objectives, this utility model provides the following technical solution:

[0010] A safe, contactless pipetting device includes a suction nozzle and a liquid tube connector. The inner wall of the liquid tube connector is fitted with a sealing sleeve, and a squeezing assembly is provided on one side of the liquid tube connector. The squeezing end of the squeezing assembly is in contact with the outer wall of the sealing sleeve.

[0011] The top of the liquid pipe connector is connected to a filter connector, the bottom of the suction head is provided with an opening, and a pressure relief connector is fixed to the opening end. The bottom of the pressure relief connector is connected to the top of the filter connector. A microbial filter membrane is provided between the filter connector and the pressure relief connector. A one-way air valve and a pressure relief structure are arranged sequentially on the outer wall of the pressure relief connector.

[0012] Furthermore, it also includes a pipette with liquid level markings, the top of which is inserted into a sealing sleeve and abuts against the sealing sleeve;

[0013] The extrusion assembly includes an extrusion plate and a bolt rod that is threadedly connected to the liquid pipe connector. The inner wall of the liquid pipe connector has a groove, and the extrusion plate is slidably inserted into the groove.

[0014] The liquid pipe connector has a limiting port that communicates with the slide groove. A limiting rod that matches the limiting port is fixed to one side of the extrusion plate. The free end of the limiting rod is slidably inserted into the limiting port.

[0015] One end of the bolt rod extends into the groove and contacts the extrusion plate.

[0016] Furthermore, the side of the extrusion plate away from the bolt rod is arc-shaped and fits the inner wall of the liquid pipe joint;

[0017] The arc-shaped side of the extrusion plate contacts the outer wall of the sealing sleeve.

[0018] Furthermore, the filter connector is threaded onto the top of the liquid pipe connector, the microbial filter membrane is placed on the top of the filter connector, the top of the filter connector is threaded onto the bottom of the pressure relief connector, and after the filter connector and the pressure relief connector are threaded together, the microbial filter membrane is sandwiched between the filter connector and the pressure relief connector.

[0019] Furthermore, a first sealing ring is fitted on the outer wall of the liquid pipe connector, and the bottom of the filter connector abuts against the first sealing ring.

[0020] The outer wall of the filter connector is fitted with a second sealing ring, and the bottom of the pressure relief connector abuts against the second sealing ring.

[0021] Furthermore, the one-way valve is threadedly connected to one side of the pressure relief connector, and the inlet end of the one-way valve is connected to the inner cavity of the pressure relief connector. The outlet end of the one-way valve is threadedly connected to a sealing cap, and the same connecting rope is fixed to the outer wall of the one-way valve and the sealing cap.

[0022] Furthermore, the pressure relief structure includes an air intake pipe. One end of the air intake pipe is fixedly connected to the pressure relief connector and communicates with the inner cavity of the pressure relief connector. An air intake hole is opened at the center of the other end of the air intake pipe. An air intake nut is threadedly connected to the end of the air intake pipe near the air intake hole. A pair of insert tubes are fixedly connected to the inner wall of the air intake nut. The free end of the pair of insert tubes is inserted into the air intake hole. Air intake slots are opened on both the pair of insert tubes and the air intake nut. The air intake hole is connected to the outside through the two air intake slots.

[0023] The inner wall of the intake nut is nested with a third sealing ring. When the intake nut is tightened with the intake pipe, the intake slot on the insertion tube is located in the intake pipe, and the side of the intake pipe closest to the intake hole abuts against the third sealing ring.

[0024] Furthermore, a breathable mesh is fixedly embedded in the air intake slot on the air intake nut.

[0025] Furthermore, it also includes a connection to an overflow-proof hose, the top of which is fixedly connected to a rigid connector, the top of which is inserted into a sealing sleeve and abuts against the sealing sleeve.

[0026] The top of the pipette is fixedly fitted to the bottom of the anti-overflow tubing;

[0027] Sterile isolation cotton was placed in the rigid connecting pipe.

[0028] (III) Beneficial Effects

[0029] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0030] I. In this utility model, the suction tip does not need to be separated from the pipette when venting air, nor does it need to be aligned with the pipette after squeezing and inserted. This reduces the cumbersomeness of the work and avoids the problem that when the suction tip is picked up or put down, some liquid will leak from the bottom of the pipette before the finger covers the top of the pipette or when the finger is removed from the top of the pipette. In addition, when placing the suction tip, it is not possible to accurately insert the suction tip into the top of the pipette in one go. If it is not placed correctly in one go, most of the liquid will leak from the bottom of the pipette.

[0031] II. In this invention, as the air inlet nut rotates, the area exposed by the air inlet slot from the air inlet hole increases, leading to a higher air intake speed and a faster liquid discharge speed. Operators can dynamically rotate the air inlet nut based on the difference between the liquid level and the target level after the suction process. If the difference is too high, the nut can be rotated several more times to increase the area exposed by the air inlet slot from the air inlet hole, thereby increasing the air intake and accelerating the liquid discharge. When the difference between the liquid level and the target level gradually decreases, the air inlet nut can be rotated in the opposite direction to reduce the area exposed by the air inlet slot from the air inlet hole, reducing the air intake and lowering the liquid discharge speed. This prevents the liquid from being discharged too quickly and ending up at the bottom of the target level, necessitating a repeat suction process.

[0032] Third, in this utility model, when not in use, the exhaust end of the one-way valve can be sealed by the sealing cover to prevent external debris from blocking the exhaust end of the one-way valve. The one-way valve and the outer wall of the sealing cover are fixed with the same connecting rope. After the sealing cover is opened, the sealing cover can be limited by the connecting rope to prevent the sealing cover from being lost.

[0033] Fourth, in this utility model, when the air inlet nut is tightened with the air inlet pipe, the air inlet slot on the insertion tube is located in the air inlet pipe, and the side of the air inlet pipe near the air inlet hole abuts against the third sealing ring. The third sealing ring can block the air inlet hole, preventing outside air from entering the air inlet pipe through the air inlet hole, which would cause liquid to leak from the bottom of the pipette. Attached Figure Description

[0034] Figure 1 This is a three-dimensional schematic diagram of the entire utility model;

[0035] Figure 2 This is a schematic diagram of the assembled suction head, liquid pipe connector, filter connector, pressure relief connector, and one-way air valve in this utility model.

[0036] Figure 3 This is a cross-sectional view of the liquid pipe connector, filter connector, and pressure relief connector in this utility model;

[0037] Figure 4 This is an exploded view of the liquid pipe joint and bolt rod in this utility model;

[0038] Figure 5 This is an exploded schematic diagram of the air intake pipe and air intake nut in this utility model;

[0039] Figure 6 This is an exploded view of the intake pipe and intake nut in this utility model from another perspective;

[0040] Figure 7This is a cross-sectional view of the air intake pipe, air intake nut, and third sealing ring in this utility model;

[0041] Figure 8 The safe, contactless pipetting device provided in the third embodiment of this utility model;

[0042] Figure 9 This is the safe, contactless pipetting device provided in the fourth embodiment of the present invention;

[0043] Figure 10 This is a cross-sectional view of the anti-overflow hose and rigid pipe in this utility model.

[0044] In the diagram: 1. Pipette; 2. Suction nozzle; 3. Liquid tube connector; 301. Slide groove; 302. Limiting port; 31. Sealing sleeve; 4. Filter connector; 41. Microbial filter membrane; 42. First sealing ring; 43. Second sealing ring; 5. Pressure relief connector; 6. One-way valve; 61. Sealing cap; 62. Connecting rope; 7. Squeezing plate; 71. Bolt rod; 72. Limiting rod; 8. Air inlet pipe; 801. Air inlet hole; 802. Air inlet slot; 82. Air inlet nut; 821. Connecting tube; 822. Breathable mesh; 83. Third sealing ring; 9. Anti-overflow tubing; 91. Rigid connecting tube; 92. Sterile isolation cotton. Detailed Implementation

[0045] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0046] Example 1

[0047] like Figures 1-7 As shown, a safe transition contactless pipetting device includes a pipette 1 with a liquid level scale, a suction nozzle 2, and a pipette connector 3. The inner wall of the pipette connector 3 is fitted with a sealing sleeve 31. The top of the pipette 1 is inserted into the sealing sleeve 31 and the pipette 1 abuts against the sealing sleeve 31. A squeezing assembly is provided on one side of the pipette connector 3, and the squeezing end of the squeezing assembly contacts the outer wall of the sealing sleeve 31.

[0048] Specifically: such as Figure 4 As shown, the extrusion assembly includes an extrusion plate 7 and a bolt rod 71 threadedly connected to the liquid pipe connector 3. The inner wall of the liquid pipe connector 3 is provided with a sliding groove 301, and the extrusion plate 7 is slidably inserted into the sliding groove 301.

[0049] The liquid pipe connector 3 is provided with a limiting port 302 that communicates with the slide groove 301. A limiting rod 72 that matches the limiting port 302 is fixedly connected to one side of the extrusion plate 7. The free end of the limiting rod 72 is slidably inserted into the limiting port 302. The limiting port 302 can limit the limiting rod 72 to prevent the extrusion plate 7 from deviating to the side when sliding.

[0050] Specifically, during assembly, the extrusion plate 7 is first placed into the slide groove 301, and the limiting rod 72 is inserted along the limiting port 302. Then, the sealing sleeve 31 is fixedly attached to the inner wall of the liquid pipe connector 3. One end of the bolt rod 71 extends into the slide groove 301 and contacts the extrusion plate 7. The side of the extrusion plate 7 away from the bolt rod 71 is arc-shaped and fits the inner wall of the liquid pipe connector 3. The arc-shaped side of the extrusion plate 7 contacts the outer wall of the sealing sleeve 31. During operation, the displacement... The top of the pipette 1 is inserted into the sealing sleeve 31. The sealing sleeve 31 increases the friction and airtightness between the pipette 1 and the pipette, preventing the pipette 1 from falling off during operation. After insertion, the bolt rod 71 is rotated. Under the action of thread engagement, the bolt rod 71 pushes the extrusion plate 7 inward. At this time, the arc-shaped side of the extrusion plate 7 will extrude the sealing sleeve 31, thereby further extruding the pipette 1 and further preventing the risk of the pipette 1 falling off during operation.

[0051] When setting the bolt rod 71, the bolt rod 71 can be set at a position corresponding to the center of the extrusion plate 7, thereby further preventing the extrusion plate 7 from sliding sideways.

[0052] The top of the liquid pipe connector 3 is connected to the filter connector 4, such as... Figure 3 As shown, the filter connector 4 is threaded onto the top of the liquid pipe connector 3. The outer wall of the liquid pipe connector 3 is fitted with a first sealing ring 42. After the filter connector 4 and the liquid pipe connector 3 are tightened, the bottom of the filter connector 4 abuts against the first sealing ring 42, thereby improving the sealing performance between the filter connector 4 and the liquid pipe connector 3.

[0053] The bottom of the suction head 2 has an opening, and a pressure relief connector 5 is fixedly connected to the opening end. The bottom of the pressure relief connector 5 is connected to the top of the filter connector 4. A microbial filter membrane 41 is provided between the filter connector 4 and the pressure relief connector 5. Specifically, the filter connector 4 is threaded to the top of the liquid pipe connector 3. During installation, the microbial filter membrane 41 is first placed on the top of the filter connector 4, and then the top of the filter connector 4 is threaded to the bottom of the pressure relief connector 5. After the filter connector 4 and the pressure relief connector 5 are threaded together, the microbial filter membrane 41 is sandwiched between the filter connector 4 and the pressure relief connector 5, thereby preventing the microbial filter membrane 41 from falling off during use. At the same time, it also facilitates the later cleaning, maintenance or replacement of the microbial filter membrane 41 and improves the disassembly speed.

[0054] The outer wall of the filter connector 4 is fitted with a second sealing ring 43, and the bottom of the pressure relief connector 5 abuts against the second sealing ring 43, thereby ensuring the airtightness between the filter connector 4 and the pressure relief connector 5.

[0055] The outer wall of the pressure relief connector 5 is provided with a one-way air valve 6, specifically: the one-way air valve 6 is threadedly connected to one side of the pressure relief connector 5, and the air inlet end of the one-way air valve 6 is connected to the inner cavity of the pressure relief connector 5.

[0056] The exhaust end of the one-way valve 6 is threadedly connected to a sealing cap 61. When not in use, the sealing cap 61 can seal the exhaust end of the one-way valve 6 to prevent external debris from clogging the exhaust end of the one-way valve 6. The same connecting rope 62 is fixed to the outer wall of the one-way valve 6 and the sealing cap 61. When the sealing cap 61 is opened, the connecting rope 62 can limit the sealing cap 61 to prevent it from being lost.

[0057] Specifically, when using it, place pipette 1 into the container containing liquid. When aspirating liquid for the first time, open the sealing cap 61 and squeeze the suction head 2. At this time, part of the air in the suction head 2 will be discharged through the one-way air valve 6, and the other part will be discharged through the bottom opening of pipette 1. After releasing the suction head 2, the inside of the suction head 2 is in a negative pressure state. During the reset process, the suction head 2 will draw the liquid in the container into pipette 1. The operator can draw the required volume of liquid according to the liquid level scale on pipette 1.

[0058] If the desired liquid level is not reached on the first attempt, bring the bottom of pipette 1 into contact with the bottom of the container's inner cavity and squeeze the suction head 2 again. At this time, some air will be expelled through the one-way air valve 6, and the other part of the air will push down on the liquid in pipette 1. Since the area of ​​the opening at the bottom of pipette 1 in contact with the outside is reduced after pipette 1 comes into contact with the inner cavity of the container, the resistance of the liquid in pipette 1 when flowing out will increase. At this time, most of the air in the suction head 2 will be expelled through the one-way air valve 6. Release the suction head 2 and lift pipette 1. At this time, during the reset process of the suction head 2, the liquid in the container will be sucked into pipette 1 again. This cycle continues until the liquid sucked in pipette 1 is slightly higher than the target liquid level.

[0059] However, in special circumstances (such as when the container holding the liquid is too tall and the bottom of pipette 1 cannot contact the bottom of the container's inner cavity), the operator may not need to contact the bottom of the container's inner cavity with pipette 1. In this case, when the suction head 2 is squeezed, some air will be discharged through the one-way air valve 6, and the other part of the air will squeeze the liquid in pipette 1 downwards to expel it. At this time, the operator needs to squeeze the suction head 2 more times until the liquid drawn in pipette 1 is slightly higher than the target liquid level.

[0060] After the liquid extraction is complete, pipette 1 can be removed from the container containing the liquid. The one-way air valve 6 prevents outside air from entering the suction head 2 and the liquid connector 3, thus avoiding the leakage of liquid from pipette 1 due to the disruption of the negative pressure environment inside the suction head 2 and the liquid connector 3 caused by the entry of outside air.

[0061] The microbial filter membrane 41 prevents microorganisms in the liquid drawn into the pipette 1 from flowing into the suction head 2 with the air. If the suction head 2 is stored for a long time, microorganisms will multiply in large numbers inside the suction head 2, causing the suction head 2 to become contaminated.

[0062] Traditional liquid aspiration methods use a separate pipette 1 and aspiration tip 2. Each time the aspiration tip 2 is squeezed, it needs to be removed from the top of the pipette 1, and each time it is removed, the top of the pipette 1 must be covered with a finger to prevent liquid from leaking from the bottom of the pipette 1. However, some liquid will leak from the bottom of the pipette 1 before the finger is covered. Secondly, when it is necessary to put the aspiration tip 2 back on the top of the pipette 1, the finger must be removed and the aspiration tip 2 must be aligned with the top of the pipette 1 and inserted. This process cannot accurately insert the aspiration tip 2 into the top of the pipette 1 in one go. At this time, most of the liquid will leak from the bottom of the pipette 1, which greatly affects the efficiency of liquid aspiration.

[0063] Compared with traditional liquid aspiration methods, in this application, the aspiration nozzle 2 does not need to be separated from the pipette 1 when venting, nor does it need to be inserted into the pipette 1 after being squeezed, reducing the cumbersomeness of the work. Although a small amount of liquid will leak out from the bottom of the pipette 1 due to the inertia of the aspiration action when the aspiration nozzle 2 is squeezed, this application greatly reduces the amount of leakage compared to the large amount of leakage caused by frequent separation and insertion operations in the traditional method.

[0064] The outer wall of the pressure relief connector 5 is also provided with a pressure relief structure, specifically as follows: Figures 5-7 As shown, the pressure relief structure includes an air intake pipe 8. One end of the air intake pipe 8 is fixedly connected to the pressure relief connector 5 and communicates with the inner cavity of the pressure relief connector 5. An air intake hole 801 is opened at the center of the other end of the air intake pipe 8. An air intake nut 82 is threadedly connected to one end of the air intake pipe 8 near the air intake hole 801. A pair of insert tubes 821 is fixedly connected to the inner wall of the air intake nut 82. The free end of the pair of insert tubes 821 is inserted into the air intake hole 801. Both the pair of insert tubes 821 and the air intake nut 82 are provided with air intake slots 802. The air intake hole 801 is connected to the outside through the two air intake slots 802.

[0065] The inner wall of the air inlet nut 82 is nested with a third sealing ring 83. When the air inlet nut 82 is tightened with the air inlet pipe 8, the air inlet slot 802 on the insertion tube 821 is located in the air inlet pipe 8, and the side of the air inlet pipe 8 near the air inlet hole 801 abuts against the third sealing ring 83. The third sealing ring 83 can block the air inlet hole 801, preventing outside air from entering the air inlet pipe 8 through the air inlet hole 801, which would cause liquid to leak from the bottom of the pipette 1.

[0066] After the liquid aspiration is completed, remove pipette 1 from the container containing the liquid, and then slowly rotate the air inlet nut 82. During the rotation, the air inlet slot 802 on the pipette 821 will gradually move out from the air inlet hole 801 and connect with the outside through the air inlet slot 802 on the air inlet nut 82. At this time, outside air will enter the air inlet tube 8, so that pipette 1 is in a balanced state. At this time, the liquid in pipette 1 is discharged under the action of gravity.

[0067] As the air inlet nut 82 rotates, the area exposed by the air inlet slot 802 from the air inlet hole 801 increases, leading to a higher air intake speed and faster liquid discharge. Operators can dynamically rotate the air inlet nut 82 based on the difference between the liquid level and the target level after the suction process. If the difference is too high, the nut nut 82 can be rotated more to increase the area exposed by the air inlet slot 802 from the air inlet hole 801, increasing the air intake and accelerating liquid discharge. Conversely, as the difference decreases, the nut nut 82 can be rotated in the opposite direction to reduce the area exposed by the air inlet slot 802 from the air inlet hole 801, decreasing the air intake and reducing the liquid discharge speed. This prevents the liquid from draining too quickly and ending up at the bottom of the target level, necessitating a repeat suction process.

[0068] Compared to traditional liquid aspiration methods, the air intake volume is adjusted by rotating the air intake nut 82, thereby accurately controlling the liquid discharge speed and ensuring that the liquid in the pipette 1 reaches the target liquid level precisely.

[0069] Example 2

[0070] like Figures 1-7 As shown, this embodiment has been improved on the basis of embodiment one as follows: Furthermore, a breathable mesh 822 is fixedly embedded in the air inlet slot 802 opened on the air inlet nut 82 to prevent debris from blocking the air inlet slot 802.

[0071] Example 3

[0072] like Figure 8As shown, this embodiment has been improved on the basis of embodiment one as follows: During the manufacturing process, the worker can also fix the air inlet end of the one-way air valve 6 to the suction head 2 with adhesive. When squeezing, the suction head 2 is bent downward or the adhesive near the pressure relief connector 5 of the suction head 2 is pinched. At this time, when the suction head 2 is squeezed, the air inside it will be discharged through the one-way air valve 6, instead of being discharged into the pipette 1 through the pressure relief connector 5, thus squeezing out the liquid in the pipette 1 and further reducing the liquid leakage caused during the liquid suction process.

[0073] Compared to threading the one-way valve 6 onto the pressure relief connector 5, bonding the one-way valve 6 to the suction head 2 during manufacturing would make it difficult to disassemble later. The position of the one-way valve 6 can be chosen according to the actual situation during manufacturing.

[0074] Example 4

[0075] like Figures 1-10 As shown, this embodiment is improved upon embodiment one as follows: Further, as... Figure 9 and Figure 10 As shown, it also includes a connecting anti-overflow hose 9, with a rigid tube 91 fixedly connected to the top of the anti-overflow hose 9. The top of the rigid tube 91 is inserted into the sealing sleeve 31, and the rigid tube 91 abuts against the sealing sleeve 31.

[0076] Specifically, during installation, the rigid tube 91 is first inserted into the sealing sleeve 31, and then further fixed by the compression assembly (the specific workflow has been described in Example 1 and will not be repeated here). Then, the top of the pipette 1 is fixedly sleeved onto the bottom of the anti-overflow tube 9. A sterile isolation cotton 92 is placed in the rigid tube 91. During liquid aspiration, occasionally, due to excessive squeezing of the aspiration head 2 by the staff, the liquid level may be higher than that of the pipette 1. At this time, a small amount of liquid may splash into the anti-overflow tube 9. The sterile isolation cotton 92 can absorb the splashed liquid and prevent the liquid from splashing onto the microbial filter membrane 41, thus affecting the normal operation of the microbial filter membrane 41.

[0077] In summary, the workflow of this utility model is as follows:

[0078] When using, place pipette 1 into the container containing liquid. When aspirating for the first time, open the sealing cap 61 and squeeze the suction head 2. At this time, part of the air in the suction head 2 will be discharged through the one-way air valve 6, and the other part will be discharged through the bottom opening of pipette 1. After releasing the suction head 2, the inside of the suction head 2 is in a negative pressure state. During the reset process, the suction head 2 will draw the liquid in the container into pipette 1. The operator can draw the required volume of liquid according to the liquid level scale on pipette 1.

[0079] If the desired liquid level is not reached on the first attempt, bring the bottom of pipette 1 into contact with the bottom of the container's inner cavity and squeeze the suction head 2 again. At this time, most of the air will be expelled through the one-way valve 6, while the remaining air will push the liquid in the pipette connector 3 downwards. Since the area of ​​the opening at the bottom of the pipette connector 3 in contact with the outside is reduced after pipette 1 comes into contact with the bottom of the container's inner cavity, the resistance to the liquid flowing out of the pipette connector 3 will increase. At this time, most of the air in the suction head 2 will be expelled through the one-way valve 6. Release the suction head 2 and lift pipette 1. During the reset process of the suction head 2, the liquid in the container will be drawn into pipette 1 again. Repeat this cycle until the liquid drawn into pipette 1 is slightly higher than the target liquid level. At this time, pipette 1 can be removed from the container containing the liquid.

[0080] After removal, slowly rotate the air inlet nut 82. During the rotation, the air inlet slot 802 on the insertion tube 821 will gradually move out from the air inlet hole 801 and connect with the outside through the air inlet slot 802 on the air inlet nut 82. At this time, the outside air will enter the air inlet tube 8, so that the pipette 1 is in a balanced state. At this time, the liquid in the pipette 1 will be discharged under the action of gravity.

[0081] As the air inlet nut 82 rotates, the area exposed by the air inlet slot 802 from the air inlet hole 801 increases, leading to a faster air intake and liquid discharge. Operators can dynamically rotate the air inlet nut 82 based on the difference between the liquid level and the target level after the suction process. If the difference is too high, the air inlet nut 82 can be rotated several more times to increase the area exposed by the air inlet slot 802 from the air inlet hole 801, thereby increasing the air intake and accelerating the liquid discharge. When the difference between the liquid level and the target level gradually decreases, the air inlet nut 82 can be rotated in the opposite direction to reduce the area exposed by the air inlet slot 802 from the air inlet hole 801, reducing the air intake and decreasing the liquid discharge. Once the liquid level is level with the target level, tightening the air inlet nut 82 completes the liquid suction.

[0082] The different embodiments described above can be combined, substituted, or used in combination with each other.

[0083] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0084] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A safe, contactless pipetting device, comprising a suction tip (2), characterized in that: It also includes a liquid pipe connector (3), the inner wall of which is fitted with a sealing sleeve (31), and a squeezing assembly is provided on one side of the liquid pipe connector (3), the squeezing end of the squeezing assembly is in contact with the outer wall of the sealing sleeve (31). The top of the liquid pipe connector (3) is connected to the filter connector (4), the bottom of the suction head (2) is provided with an opening, and the opening end is fixedly connected to the pressure relief connector (5). The bottom of the pressure relief connector (5) is connected to the top of the filter connector (4). A microbial filter membrane (41) is provided between the filter connector (4) and the pressure relief connector (5). A one-way air valve (6) and a pressure relief structure are provided on the outer wall of the pressure relief connector (5) in sequence.

2. The safe, contactless pipetting device according to claim 1, characterized in that: It also includes a pipette (1) with a liquid level scale, the top of which is inserted into a sealing sleeve (31) and the pipette (1) abuts against the sealing sleeve (31); The extrusion assembly includes an extrusion plate (7) and a bolt rod (71) threadedly connected to the liquid pipe connector (3). The inner wall of the liquid pipe connector (3) is provided with a sliding groove (301), and the extrusion plate (7) is slidably inserted into the sliding groove (301). The liquid pipe connector (3) is provided with a limiting port (302) that communicates with the slide groove (301). A limiting rod (72) that matches the limiting port (302) is fixedly connected to one side of the extrusion plate (7). The free end of the limiting rod (72) is slidably inserted into the limiting port (302). One end of the bolt rod (71) extends into the groove (301) and contacts the extrusion plate (7).

3. The safe, contactless pipetting device according to claim 2, characterized in that: The side of the extrusion plate (7) away from the bolt rod (71) is arc-shaped and fits the inner wall of the liquid pipe joint (3); The arc-shaped side of the extrusion plate (7) is in contact with the outer wall of the sealing sleeve (31).

4. The safe, contactless pipetting device according to claim 3, characterized in that: The filter connector (4) is threaded to the top of the liquid pipe connector (3), and the microbial filter membrane (41) is placed on the top of the filter connector (4). The top of the filter connector (4) is threaded to the bottom of the pressure relief connector (5). After the filter connector (4) and the pressure relief connector (5) are threaded together, the microbial filter membrane (41) is sandwiched between the filter connector (4) and the pressure relief connector (5).

5. The safe, contactless pipetting device according to claim 4, characterized in that: The outer wall of the liquid pipe connector (3) is fitted with a first sealing ring (42), and the bottom of the filter connector (4) abuts against the first sealing ring (42); The outer wall of the filter connector (4) is fitted with a second sealing ring (43), and the bottom of the pressure relief connector (5) abuts against the second sealing ring (43).

6. The safe, contactless pipetting device according to claim 4, characterized in that: The one-way valve (6) is threaded to one side of the pressure relief connector (5), and the air inlet of the one-way valve (6) is connected to the inner cavity of the pressure relief connector (5). The exhaust end of the one-way valve (6) is threaded to a sealing cap (61), and the same connecting rope (62) is fixed to the outer wall of the one-way valve (6) and the sealing cap (61).

7. The safe, contactless pipetting device according to claim 6, characterized in that: The pressure relief structure includes an air inlet pipe (8), one end of which is fixedly connected to a pressure relief connector (5) and communicates with the inner cavity of the pressure relief connector (5). An air inlet hole (801) is provided at the center of the other end of the air inlet pipe (8). An air inlet nut (82) is threadedly connected to one end of the air inlet pipe (8) near the air inlet hole (801). A pair of insert tubes (821) are fixedly connected to the inner wall of the air inlet nut (82). The free end of the pair of insert tubes (821) is inserted into the air inlet hole (801). An air inlet slot (802) is provided on both the pair of insert tubes (821) and the air inlet nut (82). The air inlet hole (801) is connected to the outside through the two air inlet slots (802). The inner wall of the air intake nut (82) is nested with a third sealing ring (83). When the air intake nut (82) is tightened with the air intake pipe (8), the air intake slot (802) on the insertion tube (821) is located in the air intake pipe (8), and the side of the air intake pipe (8) near the air intake hole (801) abuts against the third sealing ring (83).

8. The safe, contactless pipetting device according to claim 7, characterized in that: A breathable mesh (822) is fixedly embedded in the air inlet slot (802) opened on the air inlet nut (82).

9. The safe, contactless pipetting device according to claim 1, characterized in that: It also includes a connecting anti-overflow hose (9), the top of which is fixedly connected to a rigid tube (91), the top of which is inserted into a sealing sleeve (31) and the rigid tube (91) abuts against the sealing sleeve (31). The top of the pipette (1) is fixedly fitted to the bottom of the anti-overflow tubing (9); Sterile isolation cotton (92) is placed in the rigid tube (91).

Images