Magnetic bead electromagnetic adsorption device for magnetic bead method nucleic acid extraction process

Abstract

The utility model discloses a magnetic bead electromagnetic adsorption device for nucleic acid extraction by a magnetic bead method. Comprising a supporting frame and a magnetic attraction structure, the magnetic attraction structure comprises an electromagnet shell, the electromagnet shell is provided with one or more electromagnet coils, the lower portion of each electromagnet coil is provided with an electromagnet column, one side of the supporting frame protrudes outwards to form a support, the bottom of the support is provided with a through hole corresponding to the electromagnet column, and a nucleic acid extraction tube is placed and fixed through the through hole. The magnetic column of the electromagnet is placed in the nucleic acid extraction tube, and magnetic beads in the nucleic acid extraction tube can be adsorbed or fall off through power on and power off. After the induction coil is electrified, the electromagnet magnetic column generates stronger magnetic attraction force, the magnetic beads adsorbing nucleic acid are adsorbed on the surface of the electromagnet magnetic column, after a new washing solution or eluent is added into the nucleic acid extraction tube again, the power is cut off, the magnetic attraction force of the electromagnet magnetic column disappears, the magnetic beads are mixed with the washing solution or eluent again, and the nucleic acid extraction tube is separated from the washing solution or eluent. Washing of nucleic acid impurities or nucleic acid elution is completed.

Description

Technical Field

[0001] This utility model relates to a magnetic bead electromagnetic adsorption device for nucleic acid extraction using the magnetic bead method, belonging to the field of nucleic acid extraction technology. Background Technology

[0002] Magnetic bead-based nucleic acid extraction is a technique that utilizes the specific binding of nucleic acids to specific functional groups modified on the surface of magnetic microspheres (magnetic beads), thereby achieving the extraction and purification of nucleic acids from biological samples. Its basic principle is: 1. Specific functional groups are modified on the surface of magnetic beads; 2. Under specific buffer solution conditions, nucleic acid molecules bind to the functional groups on the surface of the magnetic beads, thus being adsorbed onto the surface; 3. A magnetic field generated by a magnetic device separates the adsorbed nucleic acid magnetic beads from the reaction system, while other impurities remain in the solution; 4. The adsorbed nucleic acid magnetic beads are transferred to an elution buffer. Under appropriate conditions, the binding force between nucleic acid molecules and the surface of the magnetic beads weakens, and the nucleic acids are released from the surface, thus achieving nucleic acid elution and purification. Its basic operational steps include sample lysis, nucleic acid adsorption, magnetic separation, washing, and nucleic acid elution. It can be used to extract nucleic acids from biological samples such as blood, tissues, cells, and swabs. It is widely used in clinical diagnostics, molecular biology research, forensic medicine, environmental monitoring, and food safety testing.

[0003] Traditional laboratory procedures involve using a magnetic rack equipped with a strong magnet to attract magnetic beads from the outside of a nucleic acid extraction tube, adsorbing the beads onto one side of the tube wall using magnetic attraction. The remaining liquid is then carefully aspirated with a pipette. Because the extraction tube diameter is small, the operator must be extremely careful to avoid the adsorbed beads and completely remove the residual liquid. This requires a high level of skill and is prone to accidentally pulling away the beads, affecting the extraction efficiency. Furthermore, using a strong magnet on the outside of the extraction tube often results in incomplete adsorption of the beads. Additionally, during nucleic acid elution, operators often tilt the magnetic rack to ensure effective adsorption, allowing one side of the magnet to fully adsorb the beads and preventing residual beads from affecting the quality of the nucleic acid in the eluent. However, tilting the rack leaves eluent residue on the surface of the beads, leading to incomplete elution and affecting the quality of the extracted nucleic acid. Therefore, there is an urgent need to develop an easy-to-use adsorption device. Utility Model Content

[0004] Purpose of this utility model: The purpose of this utility model is to provide a magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads, thereby overcoming the drawbacks of conventional magnetic rack adsorption of magnetic beads, such as high operational requirements, susceptibility to errors, and impact on nucleic acid washing and elution efficiency. This device can be inserted into the nucleic acid extraction tube, ensuring sufficient adsorption of the magnetic beads and avoiding the problem of insufficient adsorption affecting the quality of nucleic acid extraction. Simultaneously, after sufficient adsorption of the magnetic beads, this device can lift the nucleic acid elution solution or washing solution, facilitating subsequent processing of the nucleic acid washing and elution solutions, and avoiding the drawbacks of high operational requirements, susceptibility to errors, and the tendency for magnetic beads to remain during nucleic acid elution.

[0005] Technical Solution: The present invention discloses an electromagnetic adsorption device for nucleic acid extraction using magnetic beads, comprising a support frame and a magnetic structure. The magnetic structure includes an electromagnet shell, inside which are one or more electromagnet coils. Each electromagnet coil has a downward-facing electromagnet column inside. Wires are connected to the outside of the electromagnet coils. A support bracket protrudes outward on one side of the support frame, and a through hole is provided at the bottom of the support bracket corresponding to the electromagnet column. The nucleic acid extraction tube is placed and fixed through the through hole. The electromagnet column is placed in the nucleic acid extraction tube. When the electromagnet coil is energized by the wires, the magnetic beads in the nucleic acid extraction tube are electromagnetically attracted to the electromagnet column. When the electromagnet coil is de-energized, the magnetic beads detach from the surface of the electromagnet column.

[0006] Furthermore, a base is provided at the bottom of the support frame.

[0007] Furthermore, an iron block is provided inside the electromagnet's outer shell, and one or more holes are provided on the iron block. Each hole contains an electromagnet coil wound on a frame, and an electromagnet magnetic column is inserted downwards into the middle of the frame.

[0008] Furthermore, the distance between the top of the bracket and the support frame is greater than the length of the electromagnet column.

[0009] Furthermore, the distance between the top of the bracket and the base is greater than the length of the electromagnet column.

[0010] Furthermore, the upper inner side of the support frame and the upper inner side of the bracket are symmetrically provided with "L"-shaped grooves, and the lower ends of the left and right sides of the electromagnet shell are provided with inverted "L"-shaped grooves. The "L"-shaped grooves and the inverted "L"-shaped grooves can be placed in close contact.

[0011] Furthermore, the number of electromagnet coils is three or more.

[0012] Furthermore, the support frame is made of resin or plastic.

[0013] Furthermore, the skeleton is a nylon rod.

[0014] Furthermore, the electromagnet has a length of 50-200mm, a width of 30-90mm, a thickness of 20-50mm, and an inverted "L" shape with a top width of 5-10mm.

[0015] Furthermore, the diameter of the electromagnet coil is 10-50mm.

[0016] Furthermore, the diameter of the electromagnet column is 1-6mm, and the length is 40-60mm.

[0017] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages:

[0018] (1) This utility model generates a strong magnetic attraction force through electromagnetic induction magnetic rod, which can be inserted into the nucleic acid extraction solution for magnetic bead adsorption during use, and the adsorption effect is guaranteed.

[0019] (2) In this invention, magnetic attraction is generated by passing an electric current through the nucleic acid extract to adsorb magnetic beads. When the electromagnetic attraction is cut off, the magnetic beads are released and the magnetic beads are mixed with the extract.

[0020] (3) During use, this utility model provides structural support by setting up a support frame. The magnetic rod after adsorbing the magnetic beads can be detached from the extraction liquid and placed on the support plate, which is very convenient for subsequent processing of the extraction liquid. It has low operational requirements, is not prone to errors, and ensures the quality of nucleic acid extraction. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the magnetic bead electromagnetic adsorption device for nucleic acid extraction using the magnetic bead method of this utility model.

[0022] Figure 2 This is a schematic diagram of the magnetic bead electromagnetic adsorption device of the present invention after removing the electromagnet shell;

[0023] In the diagram: 1 is the support frame, 2 is the electromagnet coil, 3 is the electromagnet shell, 4 is the base, 5 is the nucleic acid extraction tube, 6 is the through hole, 7 is the electromagnet column, and 8 is the support.

[0024] Figure 3 This is a schematic diagram of the electromagnetic adsorption device for magnetic beads used in the magnetic bead method of nucleic acid extraction, where the electromagnet column is placed in the nucleic acid extraction tube 5 for magnetic bead adsorption.

[0025] Figure 4 This is a schematic diagram of the electromagnetic adsorption device for magnetic beads used in the magnetic bead nucleic acid extraction process of this utility model, in which the electromagnet column fully adsorbs the magnetic beads and places them on the support.

[0026] Figure 5This is a schematic diagram of the magnetic attraction structure of the magnetic bead electromagnetic adsorption device used in the magnetic bead nucleic acid extraction process of this utility model, where A is the top view, B is the left view, and C is the front view;

[0027] Figure 6 This is a schematic diagram of the support frame of the magnetic bead electromagnetic adsorption device for the magnetic bead nucleic acid extraction process of this utility model. In the diagram, A is a perspective view, B is a top view, C is a front view, and D is a left view. Detailed Implementation

[0028] The technical solution of this utility model will be further described below with reference to the accompanying drawings.

[0029] Example 1

[0030] like Figure 1-2 As shown, the magnetic bead electromagnetic adsorption device for nucleic acid extraction using the magnetic bead method of this utility model includes a support frame 1 and a magnetic adsorption structure. The magnetic adsorption structure includes an electromagnet shell 3, an iron block inside the electromagnet shell 3, and three holes evenly distributed on the iron block. Each hole contains an electromagnet coil 2 wound on a frame (nylon rod). An electromagnet magnetic column 7 is inserted downwards into the middle of the frame. Wires are connected to the outside of the electromagnet coil 2. A base 4 is provided at the bottom of the support frame 1, and a bracket 8 protrudes outwards from one side of the support frame 1. A through hole 6 is provided at the bottom of the bracket 8 corresponding to the electromagnet magnetic column 7 to facilitate the placement and fixation of the nucleic acid extraction tube. The nucleic acid extraction tube is placed and fixed through the through hole 6. The electromagnet magnetic column 7 is placed in the nucleic acid extraction tube 5. When the electromagnet coil 2 is energized by the wire, the magnetic beads in the nucleic acid extraction tube 5 are electromagnetically attracted to the electromagnet magnetic column 7. When the electromagnet coil 2 is de-energized, the magnetic beads fall off the surface of the electromagnet magnetic column 7.

[0031] The bracket 8 has upward protrusions on both sides. The inner upper part of the support frame 1 and the inner upper part of the protrusions of the bracket 8 are symmetrically provided with "L"-shaped grooves. The lower left and right sides of the electromagnet housing 3 have inverted "L"-shaped grooves. The "L"-shaped grooves and the inverted "L"-shaped grooves can fit together, allowing the electromagnet housing 3 to be placed precisely on either the support frame 1 or the bracket 8 (the electromagnet housing 3 is placed on the bracket 8 during magnetic attraction and temporarily placed on the support frame 1 after magnetic attraction). The distance between the top of the bracket 8 and the support frame 1 is greater than the length of the electromagnet column 7, and the distance between the upper end of the protrusion of the bracket 8 and the base 4 is greater than the length of the electromagnet column 7.

[0032] like Figure 5-6As shown, the support frame 1 is made of resin. The electromagnet housing 3 is 180mm long, 60mm wide, and 30mm thick. The top of the inverted "L" shape is 5mm wide. The diameter of the electromagnet coil 2 is 50mm. The diameter of the electromagnet magnetic column 7 is 5mm and the length is 55mm. The support frame 1 is 60mm wide, and the height from the top of the support frame 1 to the bottom of the base 4 is 134.5mm. The thickness of the base 4 and the bracket 8 is 10mm. The width of the bracket 8 is 60mm. The height from the top of the protruding part of the bracket 8 to the support frame 1 is 54.5mm, and the height of the protruding parts on both sides of the bracket 8 is 20mm. The diameter of the through hole 6 is 12mm, and the height from the bottom of the through hole 6 to the bottom of the base 4 is 40mm.

[0033] Working principle:

[0034] like Figure 3 As shown, during the washing process of nucleic acid extraction, the nucleic acid extraction tube 5, containing washing solution and magnetic beads, is placed on the support 8. After energizing the electromagnet coil 2 through the wire, the electromagnet column 7 with magnetic attraction structure is inserted into the nucleic acid extraction tube 5 and left to stand for 10 seconds. Under the action of magnetic attraction, the magnetic beads in the nucleic acid extraction tube 5 gather on the surface of the electromagnet column 7, and the magnetic beads separate from the washing solution.

[0035] like Figure 4 As shown, the magnetic structure is temporarily placed on the support frame 1. After pouring out the washing solution from the nucleic acid extraction tube 5, new washing solution is added. The electromagnet column 7 is then inserted back into the nucleic acid extraction tube 5. The electromagnet coil 2 is de-energized, and the magnetic bead detaches from the surface of the electromagnet column 7 after losing its magnetic attraction and enters the washing solution.

Claims

1. A magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads, characterized in that, The device includes a support frame (1) and a magnetic structure. The magnetic structure includes an electromagnet shell (3), which contains one or more electromagnet coils (2). Each electromagnet coil (2) has an electromagnet column (7) facing downward inside. The electromagnet coil (2) is connected to an electric wire. One side of the support frame (1) has a bracket (8) protruding outward. The bottom of the bracket (8) has a through hole (6) corresponding to the electromagnet column (7). The nucleic acid extraction tube is placed and fixed through the through hole (6). The electromagnet column (7) is placed in the nucleic acid extraction tube. When the electromagnet coil (2) is energized by the electric wire, the magnetic beads in the nucleic acid extraction tube can be electromagnetically attracted to the electromagnet column (7). When the electromagnet coil (2) is de-energized, the magnetic beads can fall off the surface of the electromagnet column (7).

2. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The bottom of the support frame (1) is provided with a base (4).

3. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, An iron block is provided inside the electromagnet shell (3), and one or more holes are provided on the iron block. Each hole contains an electromagnet coil (2) wound on the frame, and an electromagnet magnetic column (7) is inserted downward in the middle of the frame.

4. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The distance between the top of the bracket (8) and the support frame (1) is greater than the length of the electromagnet column (7).

5. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 2, characterized in that, The distance between the top of the bracket (8) and the base (4) is greater than the length of the electromagnet column (7).

6. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The upper inner side of the support frame (1) and the upper inner side of the bracket (8) are symmetrically provided with "L"-shaped grooves. The lower ends of the left and right sides of the electromagnet shell (3) are provided with inverted "L"-shaped grooves. The "L"-shaped grooves and the inverted "L"-shaped grooves can be placed in close contact.

7. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The number of electromagnet coils (2) is more than 3.

8. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 3, characterized in that, The support frame (1) is made of resin or plastic, and the skeleton is made of nylon rods.

9. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The length of the electromagnet shell (3) is 50-200mm, the width is 30-90mm, the thickness is 20-50mm, and the top width of the inverted "L" shape is 5-10mm.

10. The magnetic bead electromagnetic adsorption device for nucleic acid extraction using magnetic beads according to claim 1, characterized in that, The diameter of the electromagnet coil (2) is 10-50mm, and the diameter of the electromagnet column (7) is 1-6mm, and the length is 45-60mm.

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