Suction device of culture solution for cell culture

Abstract

The utility model relates to the technical field of cell culture, and discloses a suction device of a culture solution for cell culture, which comprises a culture solution storage tank, the top of the culture solution storage tank is in threaded connection with a machine cover, the top of the machine cover is provided with a driving motor, the culture solution storage tank is internally provided with a solution storage inner container, and the solution storage inner container is internally provided with a driving motor. A separation inner container is installed in the liquid storage inner container, adsorption equipment is installed on the inner wall of an inner cavity of the culture solution storage tank and comprises a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is located on the outer side of the culture solution storage tank, and the liquid outlet pipe is located in the culture solution storage tank; and the horizontal height of the liquid discharge pipe is higher than the top of the liquid storage liner. According to the suction device of the culture solution for cell culture, the culture solution storage tank is used for storing the culture solution, and the constant-temperature machine base arranged at the bottom can ensure that the culture solution in the solution storage liner is at the optimal temperature, so that the suction device can meet the function of liquid storage.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely is a kind of cell culture with culture solution's suction device. BACKGROUND

[0002] Cell culture refers to the simulation of in-vivo environment in vitro, so that it survives, grows, reproduces and maintains the main structure and function. Cell culture is also called cell cloning technology, and the formal name in biology is cell culture technology. Whether for the whole biological engineering technology or one of the biological cloning technology, cell culture is an indispensable process, and cell culture itself is the large-scale cloning of cells. Cell culture technology can be a simple single cell or a few differentiated multicellular by a large number of culture from a cell, which is an essential part of cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and the signal transduction of cells, cell synthesis and metabolism, cell growth and proliferation can be studied.

[0003] Cell culture medium is not only the basic substance for supplying cells with nutrients and promoting cell reproduction and proliferation, but also the survival environment for cell growth and reproduction.

[0004] The utility model provides a kind of cell culture with culture solution's suction device, with the preparation and recovery adsorption of providing culture solution, and provide constant temperature technology, can satisfy the timely use of culture solution in existing culture process. SUMMARY

[0005] The utility model provides the following technical scheme: a kind of cell culture with culture solution's suction device, including culture solution storage tank, the top of the culture solution storage tank is screw thread connection with machine cover, the top of the machine cover is installed with drive motor, the inside of the culture solution storage tank is equipped with liquid storage inner bag, the inside of the liquid storage inner bag is installed with separation inner bag, adsorption equipment is installed in the inner wall of the culture solution storage tank inner chamber, the adsorption equipment includes liquid inlet pipe and liquid outlet pipe, the liquid inlet pipe is located on the outside of culture solution storage tank, the liquid outlet pipe is located in the inside of culture solution storage tank, and the horizontal height of liquid outlet pipe is higher than the top of liquid storage inner bag;

[0006] The inner side of the liquid storage container is connected with a partition plate, the separation container is installed on the inner side of the partition plate, and the outer sides of the separation container and the partition plate are respectively provided with a first opening and a second opening, the first opening and the second opening are staggered, the inside of the separation container is installed with a filter plate, the surface of the filter plate is provided with a filter opening, the middle part of the filter plate is provided with a connecting channel, the connecting channel extends to the bottom of the separation container, the output rod of the driving motor is connected with the bottom of the separation container through the connecting channel, and the bottom of the separation container is provided with a connecting end.

[0007] Preferably, the outer side of the liquid inlet pipe is connected with a hose, and the other end of the hose is installed with a suction head.

[0008] Preferably, the liquid outlet pipe is a detachable hose, and the connection mode of the liquid outlet pipe and the adsorption equipment is screw connection.

[0009] Preferably, the front side of the culture solution storage tank is installed with a control panel, the inside of the culture solution storage tank is installed with a thermostat base, and the liquid storage container is placed above the thermostat base.

[0010] Preferably, the filter plate in the separation container has four groups, the filter plates in each group are arranged at equal intervals from top to bottom, the filter openings on the surface of each group of filter plates are staggered, and the bottom of the separation container is provided with a through hole.

[0011] Preferably, the liquid storage container is a ceramic container structure, the inside of the ceramic container is formed with a liquid storage cavity, and the separation container and the partition plate are alloy structures.

[0012] The utility model has the following beneficial effects:

[0013] 1. The culture solution suction device for cell culture stores the culture solution through the culture solution storage tank, the thermostat base arranged at the bottom can ensure that the culture solution in the liquid storage container is at the optimum temperature, so that the suction device can meet the liquid storage function.

[0014] 2. The culture solution suction device for cell culture can meet the preparation and recovery of the culture solution, the preparation materials of the culture solution can be mixed in the storage tank through the adsorption equipment, so that new culture solution is obtained, and the culture solution that is not used in the adsorption equipment during use can be recovered, the filter plate and the opening in the inside can reasonably flow the culture solution, so that the activity of the liquid material is ensured, and a certain filtering effect is also achieved. DETAILED DESCRIPTION

[0015] Fig. 1 It is a structural appearance drawing of the utility model;

[0016] Fig. 2 It is the schematic diagram of the liquid storage inner container structure of the utility model;

[0017] Fig. 3 It is the top view of the liquid storage inner container structure of the utility model.

[0018] In the figure: 1, culture solution storage tank; 2, machine cover; 3, driving motor; 4, liquid inlet pipe; 5, hose; 6, suction head; 7, liquid storage inner container; 8, separation inner container; 9, partition plate; 10, first opening; 11, second opening; 12, filter plate; 13, filter port; 14, connecting channel; 15, control panel; 16, liquid storage cavity; 17, adsorption equipment; 18, liquid discharge pipe; 19, constant temperature machine base. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0020] Please refer to Figs. 1-3 A culture solution suction device for cell culture, comprising a culture solution storage tank 1, a machine cover 2 is threadedly connected to the top of the culture solution storage tank 1, the machine cover 2 is separated from the machine body through thread connection, so that the culture solution is convenient to take out, a driving motor 3 is installed on the top of the machine cover 2, the driving motor 3 controls the slow rotation of the internal separation inner container 8, so that a certain filtering effect can be achieved, a liquid storage inner container 7 is arranged in the culture solution storage tank 1, the separation inner container 8 is installed in the liquid storage inner container 7, an adsorption equipment 17 is installed on the inner wall of the culture solution storage tank 1, the adsorption equipment 17 comprises a liquid inlet pipe 4 and a liquid discharge pipe 18, the liquid inlet pipe 4 is located on the outside of the culture solution storage tank 1, a hose 5 is connected to the outside of the liquid inlet pipe 4, the other end of the hose 5 is provided with a suction head 6, the suction head 6 is a detachable joint, the detachable joint is convenient to replace regularly, and bacteria are easy to produce after long-time use of the same suction head, so that the use of the culture solution is affected, the liquid discharge pipe 18 is located in the culture solution storage tank 1, the liquid discharge pipe 18 is a detachable hose, the connection mode of the liquid discharge pipe 18 and the adsorption equipment 17 is thread connection, and the horizontal height of the liquid discharge pipe 18 is higher than the top of the liquid storage inner container 7, the height is arranged, so that the culture solution is conveniently flowed into the liquid storage cavity 16 through the physical mode;

[0021] The inner side of the liquid storage inner container 7 is connected with a partition plate 9, the separation inner container 8 is installed on the inner side of the partition plate 9, the liquid storage inner container 7 is a ceramic inner container structure, a liquid storage cavity 16 is formed in the ceramic inner container, the separation inner container 8 and the partition plate 9 are alloy structures, and the outer sides of the separation inner container 8 and the partition plate 9 are respectively provided with a first opening 10 and a second opening 11, the first opening 10 and the second opening 11 are staggered, the inside of the separation inner container 8 is installed with a filter plate 12, the surface of the filter plate 12 is provided with a filter opening 13, and the middle of the filter plate 12 is provided with a connecting channel 14 extending to the bottom of the separation inner container 8, so as to meet the preparation of the culture solution and the recovery of the culture solution, the preparation materials of the culture solution can be mixed by being absorbed by the adsorption equipment 17 in the storage tank, so as to obtain new culture solution, and the culture solution not used in the use process can also be adsorbed by the adsorption equipment 17 to be recovered, the filter plate 12 and the opening can reasonably flow the culture solution, so as to ensure the activity of the liquid material and also play a certain filtering role, the output rod of the driving motor 3 is connected with the bottom of the separation inner container 8 through the connecting channel 14, the filter plate 12 in the separation inner container 8 has four groups, the four groups of filter plates 12 are equidistantly arranged from top to bottom, the filter openings 13 on the surface of each group of filter plates 12 are staggered, and the bottom of the separation inner container 8 is provided with a through hole, the bottom of the separation inner container 8 is provided with a connecting end, and the separation inner container 8 is movably connected with the liquid storage inner container 7 through the connecting end.

[0022] The front of the culture solution storage tank 1 is installed with a control panel 15, the control panel 15 is used for controlling the opening and closing of the equipment, can control the internal temperature, and ensures that the culture solution can meet the cell culture, the inside of the culture solution storage tank 1 is installed with a constant temperature machine base 19, the liquid storage inner container 7 is placed above the constant temperature machine base 19, the culture solution is stored by the culture solution storage tank 1, and the constant temperature machine base 19 arranged at the bottom can ensure that the culture solution in the liquid storage inner container 7 is at the best temperature, so that the suction device can meet the function of liquid storage.

[0023] Working principle, when the culture solution adsorption equipment is used, the starting of the adsorption equipment 17 is controlled through the control panel 15, the adsorption equipment 17 absorbs the culture solution through the external hose 5 and the suction head 6, discharges the culture solution into the separation inner container 8 through the internal liquid discharge pipe 18, and preliminarily filters the culture solution through the filter plate 12 and the filter opening 13, under the action of the driving motor 3 controlling the rotation of the separation inner container 8, the second opening 11 of the partition plate 9 and the first opening 11 thereof are matched to filter the culture solution again, so that the filtered culture solution enters the liquid storage inner container 7 to be stored, when the culture solution needs to be used, the culture solution is discharged by rotating and opening the machine cover 2, taking out the liquid storage inner container 7 in the inside.

[0024] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0025] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A cell culture medium suction device for cell culture, comprising a culture medium storage tank (1), characterized in that: The top of the culture solution storage tank (1) is threadedly connected with a machine cover (2), the top of the machine cover (2) is provided with a driving motor (3), the inside of the culture solution storage tank (1) is provided with a liquid storage inner container (7), the inside of the liquid storage inner container (7) is provided with a separation inner container (8), the inner wall of the inner cavity of the culture solution storage tank (1) is provided with an adsorption device (17), the adsorption device (17) comprises a liquid inlet pipe (4) and a liquid outlet pipe (18), the liquid inlet pipe (4) is located outside the culture solution storage tank (1), the liquid outlet pipe (18) is located inside the culture solution storage tank (1), and the horizontal height of the liquid outlet pipe (18) is higher than the top of the liquid storage inner container (7); The inside of the liquid storage inner container (7) is connected with a partition plate (9), the separation inner container (8) is mounted on the inside of the partition plate (9), the outside of the separation inner container (8) and the partition plate (9) is respectively provided with a first opening (10) and a second opening (11), the first opening (10) and the second opening (11) are staggered, the inside of the separation inner container (8) is provided with a filter plate (12), the surface of the filter plate (12) is provided with a filter opening (13), the middle of the filter plate (12) is provided with a connecting channel (14), the connecting channel (14) extends to the bottom of the separation inner container (8), the output rod of the driving motor (3) is connected with the bottom of the separation inner container (8) through the connecting channel (14), and the bottom of the separation inner container (8) is provided with a connecting end, and the separation inner container (8) is movably connected with the liquid storage inner container (7) through the connecting end.

2. The cell culture medium suction device according to claim 1, wherein: The outside of the liquid inlet pipe (4) is connected with a hose (5), the other end of the hose (5) is provided with a suction head (6), and the suction head (6) is a detachable joint.

3. The cell culture medium suction device according to claim 1, wherein: The liquid outlet pipe (18) is a detachable hose, and the connection mode of the liquid outlet pipe (18) and the adsorption device (17) is screw connection.

4. The cell culture medium suction device according to claim 1, wherein: The front of the culture solution storage tank (1) is provided with a control panel (15), the inside of the culture solution storage tank (1) is provided with a constant temperature machine base (19), and the liquid storage inner container (7) is placed above the constant temperature machine base (19).

5. The cell culture medium suction device according to claim 1, wherein: The filter plate (12) in the separation inner container (8) has four groups, the four groups of filter plates (12) are equidistantly arranged from top to bottom, the filter openings (13) on the surface of each group of filter plates (12) are staggered, and the bottom of the separation inner container (8) is provided with a through hole.

6. The cell culture medium suction device according to claim 1, wherein: The liquid storage inner container (7) is a ceramic inner container structure, the inside of the ceramic inner container is formed with a liquid storage cavity (16), and the separation inner container (8) and the partition plate (9) are alloy structures.

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