Magnetic bead method nucleic acid extraction device convenient to operate

Abstract

The utility model discloses a convenient-to-operate nucleic acid extraction device adopting a paramagnetic particle method. The convenient-to-operate nucleic acid extraction device comprises a shell, a tip is fixedly arranged at one end of the shell, an end cover is fixedly mounted at one end, away from the tip, of the shell, a support is slidably mounted in the shell, a replaceable magnetic device is arranged at one end, close to the tip, of the shell, a magnetic module is arranged at one end, close to the end cover, of the shell, and a button is mounted on the support. According to the utility model, the first magnetic bar is arranged on the bracket, the second magnetic bar is inserted into the sharp end in a magnetic attraction manner and is connected with the first magnetic bar, after one sample is extracted, the magnetic bar sleeve can be directly pulled out, the second magnetic bar is pulled out, at the moment, a new second magnetic bar can be replaced, and a new disposable magnetic bar sleeve is mounted, so that the next sample can be extracted; due to the design of the replaceable magnetic rod sleeve and the second magnetic rod, continuous anti-pollution detection can be carried out, and the magnetic rod sleeves and the second magnetic rods with different specifications can be freely replaced according to the sizes of the used magnetic beads, so that the applicability is improved.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, specifically to a convenient magnetic bead-based nucleic acid extraction device. Background Technology

[0002] A telescopic magnetic rod pen for magnetic bead extraction, purification, and separation, with announcement number CN221797464U, includes a cylinder, a push rod, a drive mechanism, and a magnetic element. A disposable cap is fitted to the lower end of the cylinder to cover the magnetic element. The drive mechanism, located on the cylinder, drives the push rod to slide back and forth, causing the magnetic element to slide back and forth inside the cap. This simplified magnetic bead extraction method by using a drive mechanism to move the magnetic element up and down, significantly improving the extraction of nucleic acids in small quantities while reducing the skill requirements for operators. The disclosed extraction rod pen has a simple structure, low cost, and is suitable for widespread use in laboratories.

[0003] Although the above-mentioned device is equipped with a disposable cap, it requires pushing part of the magnetic body and push rod into the test reagent during use. At this time, the magnetic body and push rod are already contaminated. If multiple different samples are processed at the same time, the magnetic body and push rod need to be disinfected after each sample extraction before they can be reused. Otherwise, the samples will be contaminated. Therefore, a convenient magnetic bead nucleic acid extraction device is needed to meet people's needs. Utility Model Content

[0004] The purpose of this invention is to provide a convenient magnetic bead-based nucleic acid extraction device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a convenient magnetic bead nucleic acid extraction device, comprising a shell; a pointed tip is fixedly provided at one end of the shell, an end cap is fixedly installed at the end of the shell away from the pointed tip, a bracket is slidably installed inside the shell, a replaceable magnetic suction device is provided at the end of the shell near the pointed tip, a magnetic suction module is provided at the end of the shell near the end cap, and a button is installed on the bracket;

[0006] Preferably, the replaceable magnetic device includes a first magnetic rod, which is fixedly installed on one end of the bracket near the tip. A second magnetic rod is magnetically attracted to one end of the first magnetic rod, and the second magnetic rod is slidably installed inside the tip. A magnetic rod sleeve is snapped into the tip.

[0007] Preferably, the bracket has a first fixing groove near the tip, one end of the first magnetic rod is fixedly installed in the first fixing groove, the magnetic rod sleeve has a through hole, and the second magnetic rod is slidably installed in the through hole.

[0008] Preferably, the tip has a slot, and the slot is interference-fitted with the magnetic rod sleeve.

[0009] Preferably, the magnetic module includes a first magnetic block and a second magnetic block, wherein the first magnetic block is fixedly mounted on the end cap and the second magnetic block is fixedly mounted on the bracket.

[0010] Preferably, the end cap has a second fixing groove, the first magnetic block is fixedly installed in the second fixing groove, and the bracket has a third fixing groove near the end cap, the second magnetic block is fixedly installed in the third fixing groove.

[0011] Preferably, the first magnetic block and the second magnetic block are magnetically attracted to each other.

[0012] Preferably, the outer casing has a limiting groove, the button is slidably installed in the limiting groove, and the button and the bracket are threaded with the same fixing screw, and the button is fixedly installed on the bracket by the fixing screw.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] (1) In this utility model, the support has a first magnetic rod, and the second magnetic rod is inserted into the tip and connected to the first magnetic rod by magnetic attraction. After the sample is extracted, the magnetic rod sleeve can be removed directly and the second magnetic rod can be pulled out. At this time, a new second magnetic rod can be replaced and a new disposable magnetic rod sleeve can be installed to start the extraction of the next sample. The design of the replaceable magnetic rod sleeve and the second magnetic rod can not only continuously prevent contamination during detection, but also allow for the free replacement of different specifications of magnetic rod sleeve and second magnetic rod according to the size of the magnetic beads used, thus expanding its applicability.

[0015] (2) When in use, the button can be toggled so that it slides in the limit groove to control the movement of the bracket. When the magnetic bead needs to be released, the button is toggled towards the end cover. When the second magnetic block on the bracket approaches the first magnetic block, it will approach and be attracted by the magnetic attraction. At this time, there is no need to manually maintain the position of the button, making the operation more convenient and faster. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of a convenient magnetic bead-based nucleic acid extraction device proposed in this utility model.

[0017] Figure 2 This is a schematic diagram of the magnetic rod sleeve and through hole structure of a convenient magnetic bead nucleic acid extraction device proposed in this utility model;

[0018] Figure 3 This is a structural diagram of the fixing screws and buttons of a convenient magnetic bead-based nucleic acid extraction device proposed in this utility model;

[0019] Figure 4This is a schematic diagram of the second and third fixing grooves of a convenient magnetic bead-based nucleic acid extraction device proposed in this utility model.

[0020] In the diagram: 1. Outer shell; 2. Replaceable magnetic device; 3. Magnetic module; 4. End cap; 5. Limiting groove; 6. Bracket; 7. Tip; 8. Fixing screw; 9. Button; 20. First magnetic rod; 21. Second magnetic rod; 22. Magnetic rod sleeve; 23. Through hole; 24. First fixing groove; 25. Slot; 30. First magnetic block; 31. Second magnetic block; 32. Second fixing groove; 33. Third fixing groove. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Example 1: Please refer to Figure 1-4 This utility model provides a technical solution: a convenient magnetic bead nucleic acid extraction device, including a shell 1; in order to design a magnetic bead extraction device with a replaceable magnetic head, a tip 7 is fixedly provided at one end of the shell 1, an end cap 4 is fixedly installed at the end of the shell 1 away from the tip 7, a bracket 6 is slidably installed inside the shell 1, a replaceable magnetic suction device 2 is provided at the end of the shell 1 near the tip 7, a magnetic suction module 3 is provided at the end of the shell 1 near the end cap 4, and a button 9 is installed on the bracket 6. The magnetic head can be replaced by the replaceable magnetic suction device 2, and the magnetic suction module 3 allows the user to use the device without continuously pressing the button.

[0023] To facilitate continuous operation by easily replacing the magnetic head, the replaceable magnetic device 2 includes a first magnetic rod 20, which is fixedly mounted on the end of the bracket 6 near the tip 7. A second magnetic rod 21 is magnetically attracted to one end of the first magnetic rod 20 and slidably mounted inside the tip 7. A magnetic rod sleeve 22 is engaged inside the tip 7. A first fixing groove 24 is formed on the end of the bracket 6 near the tip 7, and one end of the first magnetic rod 20 is fixedly mounted in the first fixing groove 24. A through hole 23 is formed on the magnetic rod sleeve 22, and the second magnetic rod 21 is slidably mounted in the through hole 23. A slot 25 is formed inside the tip 7, and the slot 25 is press-fitted with the magnetic rod sleeve 22. A limit groove 5 is formed on the outer shell 1, and the button 9 slides... The button 9 is installed in the limiting groove 5. The same fixing screw 8 is threaded onto both the button 9 and the bracket 6. The button 9 is fixed to the bracket 6 by the fixing screw 8. In use, the second magnetic rod 21 is inserted through the tip 7, and one end of the second magnetic rod 21 is brought close to the first magnetic rod 20. The second magnetic rod 21 and the first magnetic rod 20 attract each other under magnetic attraction, thus connecting the first magnetic rod 20 and the second magnetic rod 21 together. At this time, the magnetic rod sleeve 22 is inserted into the slot 25, ensuring the second magnetic rod 21 is located in the through hole 23, completing the preparatory installation. The button 9 is then moved towards the tip 7. When the button 9 is moved towards the tip 7, it will cause the limiting groove 5 to move towards the tip. 7. With one end of the magnetic rod extended from the magnetic rod sleeve 22, insert the tip of the second magnetic rod 21 into the reagent. The second magnetic rod 21, being magnetic, will attract the magnetic beads in the reagent. After attraction, remove the second magnetic rod 21 from the reagent surface and align it with the device for collecting the magnetic beads. Move button 9 towards the end cap 4. As button 9 moves towards end cap 4, it moves the support 6, causing the second magnetic rod 21 to retract into the magnetic rod sleeve 22. The magnetic beads on the second magnetic rod 21 abut against the end face of the magnetic rod sleeve 22. Once the magnetic beads detach from the second magnetic rod 21 and lose their attraction, they will fall off, thus completing the magnetic bead collection process. For easy replacement, after extraction, the magnetic rod sleeve 22 and the second magnetic rod 21 can be removed. The magnetic rod sleeve 22 and the slot 25 are interference-fitted, and can be pulled out with a little force. The second magnetic rod 21 is magnetically attracted to the first magnetic rod 20, and can also be separated from the first magnetic rod 20 with a little force. After installing the new second magnetic rod 21 and magnetic rod sleeve 22 according to the above installation scheme, a new round of extraction can be performed. There is no need to wait for sterilization time, and multiple samples can be processed continuously. Moreover, by configuring second magnetic rods 21 and magnetic rod sleeves 22 of different specifications, the device can be used to accommodate magnetic beads of different sizes, adapt to more applicable scenarios, and expand its applicability.

[0024] Example 2: As Figure 2 and Figure 4To achieve the function of eliminating the need for continuous button operation, the magnetic module 3 includes a first magnetic block 30 and a second magnetic block 31. The first magnetic block 30 is fixedly mounted on the end cap 4, and the second magnetic block 31 is fixedly mounted on the bracket 6. The end cap 4 has a second fixing groove 32, in which the first magnetic block 30 is fixedly mounted. The bracket 6 has a third fixing groove 33 near the end cap 4, in which the second magnetic block 31 is fixedly mounted. The first magnetic block 30 and the second magnetic block 31 are magnetically attracted to each other. During use, the button 9 can be tossed, causing it to slide within the limiting groove 5 to control the movement. When the movement of the bracket 6 requires the release of the magnetic bead, the button 9 is moved towards the end cover 4. When the second magnetic block 31 on the bracket 6 approaches the first magnetic block 30, it will approach and be attracted by the magnetic force. At this time, there is no need to manually maintain the position of the button 9, making the operation more convenient and faster. It is worth noting that when selecting the materials of the first magnetic block 30 and the second magnetic block 31, it is only necessary to ensure that the two can be magnetically attracted to each other when they are close. Both can be set as magnets that attract each other, or one can be set as a magnet and the other can be set as a metal material that can be magnetically attracted to it. The other features are the same as in Embodiment 1.

[0025] The working principle is as follows: During use, insert the second magnetic rod 21 through the tip 7, bringing one end of the second magnetic rod 21 close to the first magnetic rod 20. The second magnetic rod 21 and the first magnetic rod 20 will attract each other under magnetic attraction, thus connecting the first magnetic rod 20 and the second magnetic rod 21 together. Then, insert the magnetic rod sleeve 22 into the slot 25, ensuring the second magnetic rod 21 is positioned within the through hole 23, completing the preparatory installation. Move the button 9 towards the tip 7. When the button 9 is moved towards the tip 7, it will cause the limiting groove 5 to move towards one side of the tip 7. The second magnetic rod 21 is then extended from the magnetic rod sleeve 22, and its tip is inserted into the reagent. The second magnetic rod 21, being magnetic, will attract the magnetic beads in the reagent. After attraction, the second magnetic rod 21 is removed from the reagent surface and aligned with the device for collecting the magnetic beads. Button 9 is then moved towards the end cap 4. As button 9 moves towards end cap 4, it moves the support 6, causing the second magnetic rod 21 to retract into the magnetic rod sleeve 22. The magnetic beads on the second magnetic rod 21 then abut against the end face of the magnetic rod sleeve 22, and the magnetic... After the bead detaches from the second magnetic rod 21, it loses its attractive force and falls off, thus completing the separation of the magnetic bead. For easy replacement, the magnetic rod sleeve 22 and the second magnetic rod 21 can be removed after extraction. The magnetic rod sleeve 22 and the slot 25 are interference-fitted, and it can be pulled out with a little force. The second magnetic rod 21 is magnetically attracted to the first magnetic rod 20, and it can also be separated from the first magnetic rod 20 with a little force. After installing the new second magnetic rod 21 and magnetic rod sleeve 22 according to the above installation scheme, a new round of extraction can be performed. There is no need to wait for sterilization time, and multiple extractions can be performed continuously. The sample is processed, and by configuring the second magnetic rod 21 and magnetic rod sleeve 22 of different specifications, the device can be used to accommodate magnetic beads of different sizes, adapt to more applicable scenarios, and expand its applicability. When in use, the button 9 can be toggled so that the button 9 slides in the limiting groove 5 to control the movement of the bracket 6. When the magnetic bead needs to be released, the button 9 is toggled towards the end cover 4. When the second magnetic block 31 on the bracket 6 approaches the first magnetic block 30, it will approach and be attracted under the action of magnetic attraction. At this time, there is no need to manually maintain the position of the button 9, making the operation more convenient and faster.

[0026] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A convenient magnetic bead-based nucleic acid extraction device, comprising a shell (1); characterized in that: One end of the outer shell (1) is fixedly provided with a pointed tip (7), and the end of the outer shell (1) away from the pointed tip (7) is fixedly installed with an end cap (4). A bracket (6) is slidably installed inside the outer shell (1). A replaceable magnetic suction device (2) is provided at the end of the outer shell (1) near the pointed tip (7). A magnetic suction module (3) is provided at the end of the outer shell (1) near the end cap (4). A button (9) is installed on the bracket (6). The replaceable magnetic device (2) includes a first magnetic rod (20), which is fixedly installed on the bracket (6) near the tip (7). A second magnetic rod (21) is magnetically attracted to one end of the first magnetic rod (20), and the second magnetic rod (21) is slidably installed in the tip (7). A magnetic rod sleeve (22) is snapped into the tip (7).

2. The convenient magnetic bead-based nucleic acid extraction device according to claim 1, characterized in that: The bracket (6) has a first fixing groove (24) near the tip (7), and one end of the first magnetic rod (20) is fixedly installed in the first fixing groove (24). The magnetic rod sleeve (22) has a through hole (23), and the second magnetic rod (21) is slidably installed in the through hole (23).

3. The convenient magnetic bead-based nucleic acid extraction device according to claim 2, characterized in that: The tip (7) has a slot (25) inside, and the slot (25) is interference-fitted with the magnetic rod sleeve (22).

4. The convenient magnetic bead-based nucleic acid extraction device according to claim 1, characterized in that: The magnetic module (3) includes a first magnetic block (30) and a second magnetic block (31). The first magnetic block (30) is fixedly installed on the end cap (4), and the second magnetic block (31) is fixedly installed on the bracket (6).

5. The convenient magnetic bead-based nucleic acid extraction device according to claim 4, characterized in that: The end cap (4) has a second fixing groove (32), and the first magnetic block (30) is fixedly installed in the second fixing groove (32). The bracket (6) has a third fixing groove (33) at one end near the end cap (4), and the second magnetic block (31) is fixedly installed in the third fixing groove (33).

6. The convenient magnetic bead-based nucleic acid extraction device according to claim 5, characterized in that: The first magnetic block (30) and the second magnetic block (31) are magnetically attracted to each other.

7. The convenient magnetic bead-based nucleic acid extraction device according to claim 1, characterized in that: The outer casing (1) has a limiting groove (5), the button (9) is slidably installed in the limiting groove (5), the button (9) and the bracket (6) are threaded with the same fixing screw (8), and the button (9) is fixedly installed on the bracket (6) by the fixing screw (8).

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