Filter device for cell culture

Abstract

The utility model relates to the technical field of cell culture, in particular to a filtering device for cell culture, which comprises a filtering device body, a filtering shell is arranged in the filtering device body; a filter screen is arranged at the bottom of the filter shell; a control assembly is arranged outside the filtering shell; the control assembly comprises a vertical control rod, a compression spring and a positioning sliding block. The vertical slide rail is fixedly mounted on the inner wall of the filter shell; a compression spring is arranged at the bottom of the vertical sliding rail connected with the control block; the filter screen consists of a filter membrane and a support screen; the filtering membrane sequentially comprises a pre-filtering layer, a main filtering layer and a fine filtering layer from top to bottom; the filtering membranes are microporous filtering membranes with different pore diameters; the aperture of each layer of the filtering membrane is sequentially reduced from top to bottom; the supporting net is positioned below the filtering membranes, and meanwhile, the filtering pressure can be effectively dispersed through the design of multiple layers of filtering membranes, the blocking phenomenon of the filtering membranes is reduced, and the service life of the device is prolonged.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a filtration device for cell culture. Background Technology

[0002] Cell growth requires a nutrient environment. The nutrient matrix used to maintain cell growth is called culture medium. Cell culture medium is not only the basic substance that supplies nutrients to cultured cells and promotes cell reproduction and proliferation, but also the living environment for cell growth and reproduction. Culture medium includes liquid culture medium and solid culture medium.

[0003] Liquid culture involves storing filtered culture medium and cells inside a liquid culture tube. As the most basic raw material in the field of cell culture, cell culture medium is being used in increasingly larger quantities and types, and is widely used in various fields of cell biology. Self-preparation of the required cell culture medium has become the choice of most people, and sterilization filtration of the cell culture medium is an essential condition.

[0004] Cell culture medium is usually filtered through a filter screen in a filtration device. Traditional filtration devices generally have problems such as low filtration efficiency and easy clogging, which makes it difficult for cell culture medium to pass through the filter screen, affecting the filtration effect. In addition, since the filter screen is generally designed horizontally, some undribbled cell culture medium will remain on the bottom surface of the filter screen after filtration, resulting in waste of resources. Utility Model Content

[0005] The purpose of this application is to provide a filtration device for cell culture, which aims to solve the problems in the prior art.

[0006] This application provides a filtration device for cell culture, including a filtration device body; a filter housing is provided inside the filtration device body; a filter screen is provided at the bottom of the filter housing; a control component is provided outside the filter housing; the control component includes a vertical control rod, a compression spring, and a positioning slider; the positioning slider includes a control block and a driven block; the control block and the driven block are respectively located on both sides of the filter housing; one end of each positioning slider is fixedly connected to the filter housing, and the other end is slidably connected to a vertical slide rail; the vertical slide rail is fixedly installed on the inner wall of the filter housing; a compression spring is provided at the bottom of the vertical slide rail connected to the control block; a vertical control rod is fixedly provided on the control block; the vertical control rod extends from the top of the filtration device body; the filter screen is composed of a filter membrane and a support mesh; the filter membrane, from top to bottom, consists of a pre-filtration layer, a main filtration layer, and a fine filtration layer; the filter membrane is a microporous filter membrane with different pore sizes; the pore size of each layer of the filter membrane decreases from top to bottom; the support mesh is located below the filter membrane; a reinforcing barrier is provided around the support mesh.

[0007] Furthermore, the bottom of the filter device body is provided with a liquid outlet; a valve is installed at the liquid outlet; the liquid outlet is connected to a negative pressure suction filter tube.

[0008] Furthermore, the filter device body is provided with a liquid inlet at the top; the liquid inlet is connected to the top of the filter housing through a telescopic tube.

[0009] Furthermore, the top of the filter housing is provided with a removable sealing cover; the telescopic tube is connected to the sealing cover.

[0010] Furthermore, the outer surface of the reinforced enclosure is provided with external threads, and the bottom of the filter housing is provided with internal threads; the filter housing and the reinforced enclosure are connected to each other by threads.

[0011] The beneficial effects of this utility model are: this utility model can control the filter housing to shake up and down, causing cell fluid to drip down, thus avoiding waste of cell fluid, and at the same time preventing cell fluid from dripping onto the table and causing pollution when the filter housing is removed.

[0012] This invention also employs multiple layers of filter membranes with different pore sizes, which can effectively intercept particles and microorganisms of different sizes, ensuring a sterile environment for cell culture. At the same time, the multi-layer filter membrane design can effectively distribute filtration pressure, reduce filter membrane clogging, and extend the service life of the device. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model.

[0014] Figure 2 This is a schematic diagram of the filter housing structure.

[0015] Figure 3 for Figure 1 Enlarged view of the structure at point A in the middle.

[0016] Figure 4 for Figure 1 Enlarged view of the structure in section B.

[0017] Figure 5 This is a schematic diagram of the filter screen structure.

[0018] In the picture:

[0019] 1. Filter device body; 11. Vertical slide rail; 12. Liquid inlet; 13. Liquid outlet; 14. Valve; 15. Negative pressure suction pipe; 2. Filter housing; 21. Sealing cover; 22. Telescopic pipe; 3. Filter membrane; 31. Pre-filtration layer; 32. Main filtration layer; 33. Fine filtration layer; 4. Support net; 41. Reinforced enclosure; 411. External thread; 5. Vertical control rod; 6. Driven block; 7. Compression spring; 8. Control block. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] like Figures 1 to 5 A cell culture filtration device is shown, comprising a filtration device body 1; a filter housing 2 is provided inside the filtration device body 1, the material of which can be medical-grade plastic or glass that is resistant to high temperature and corrosion; a filter screen is provided at the bottom of the filter housing 2; a control assembly is provided outside the filter housing 2; the control assembly includes a vertical control rod 5, a compression spring 7, and a positioning slider; the positioning slider includes a control block 8 and a driven block 6; the control block 8 and the driven block 6 are respectively located on both sides of the filter housing 2; one end of each positioning slider is fixedly connected to the filter housing 1, and the other end is slidably connected to a vertical slide rail 11; the vertical... The slide rail 11 is fixedly installed on the inner wall of the filter housing 2; the bottom of the vertical slide rail 11 connected to the control block 8 is provided with a compression spring 7, while the other vertical slide rail 11 is not provided with a compression spring 7; a vertical control rod 5 is fixedly installed on the control block 8; the vertical control rod 5 extends from the top of the filter device body 1; the operation process of the control component is as follows: press down on the vertical control rod 5, and both the control block 8 and the driven block 6 will move down along the vertical slide rail 11. Then release the control rod 5, and the control block 8 will rebound upward under the action of the compression spring 7, thereby causing the entire filter housing 2 to shake up and down, which is conducive to the falling of residual cell liquid on the bottom surface of the filter screen.

[0022] The filter consists of a filter membrane 3 and a support mesh 4. The filter membrane 3, from top to bottom, comprises a pre-filtration layer 31, a main filtration layer 32, and a fine filtration layer 33, designed to effectively intercept particles and microorganisms of different sizes. During installation, the layers are installed sequentially and ensured to fit tightly together. The filter membrane 3 is a microporous membrane with varying pore sizes; the pore size of each layer of the filter membrane 3 decreases from top to bottom. The support mesh 4 is located below the filter membrane 3 and is surrounded by a reinforcing barrier 41. The support mesh 4 supports the filter membrane 3 to prevent deformation or breakage during use.

[0023] The filter device body 1 has a liquid outlet 13 at its bottom; a valve 14 is installed at the liquid outlet 13 so as to control the opening and closing of the liquid outlet 13; the liquid outlet 13 is connected to a negative pressure suction tube 15. The cell fluid flowing out of the liquid outlet 13 can be sucked out through the negative pressure suction tube 15.

[0024] The filter device body 1 is provided with a liquid inlet 12 at the top; the liquid inlet 12 is connected to the top of the filter housing 2 through a telescopic tube 22.

[0025] The filter housing 2 is provided with a detachable sealing cover 21 at the top; the telescopic tube 22 is connected to the sealing cover 21, and when the vertical control rod 5 is pressed down to control the entire filter housing 2 to move up and down, the telescopic tube 22 can provide movement space.

[0026] The outer surface of the reinforced enclosure 41 is provided with an external thread 411, and the bottom of the filter housing 2 is provided with an internal thread; the filter housing 2 and the reinforced enclosure 41 are connected to each other by threads, making it convenient to remove the filter membrane 3 for cleaning or replacement.

[0027] When in use, the cell fluid to be filtered is added through the inlet 12. The cell fluid enters the filter housing 2 along the telescopic tube 22 and then passes through the pre-filtration layer 31, the main filtration layer 32 and the fine filtration layer 33 in sequence to intercept particles and microorganisms of different sizes and ensure complete filtration. When there is cell fluid at the bottom of the filter screen, the filter housing 2 can be shaken up and down by the control component to make the cell fluid drip down.

[0028] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A filtration device for cell culture, characterized in that, The device includes a filter body; a filter housing is provided inside the filter body; a filter screen is provided at the bottom of the filter housing; a control assembly is provided outside the filter housing; the control assembly includes a vertical control rod, a compression spring, and a positioning slider; the positioning slider includes a control block and a driven block; the control block and the driven block are respectively located on both sides of the filter housing; one end of each positioning slider is fixedly connected to the filter housing, and the other end is slidably connected to a vertical slide rail; the vertical slide rail is fixedly installed on the inner wall of the filter housing; a compression spring is provided at the bottom of the vertical slide rail connected to the control block; a vertical control rod is fixedly provided on the control block; the vertical control rod extends from the top of the filter body; the filter screen is composed of a filter membrane and a support mesh; the filter membrane, from top to bottom, consists of a pre-filtration layer, a main filtration layer, and a fine filtration layer; the filter membrane is a microporous filter membrane with different pore sizes. The pore size of each layer of the filter membrane decreases sequentially from top to bottom; the support mesh is located below the filter membrane; and a reinforced enclosure is provided around the support mesh.

2. The cell culture filtration device according to claim 1, characterized in that, The filter device body has a liquid outlet at the bottom; a valve is installed at the liquid outlet; the liquid outlet is connected to a negative pressure suction filter tube.

3. The cell culture filtration device according to claim 2, characterized in that, The filter device body is provided with a liquid inlet at the top; the liquid inlet is connected to the top of the filter housing through a telescopic tube.

4. The cell culture filtration device according to claim 3, characterized in that, The filter housing is provided with a removable sealing cover at the top; the telescopic tube is connected to the sealing cover.

5. The cell culture filtration device according to claim 1, characterized in that, The outer surface of the reinforced enclosure is provided with external threads, and the bottom of the filter housing is provided with internal threads. The filter housing and the reinforced enclosure are connected to each other by threads.

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