Biological electron microscope sample dehydration device

Abstract

The utility model discloses a biological electron microscope sample dehydration device, and belongs to the field of biological sample dehydration devices.The dehydration device comprises a support, a positioning sleeve axially moving on the support, a reagent tube inserted into the positioning sleeve and a swing structure used for driving the reagent tube to swing to accelerate mixing of water and ethyl alcohol; the feeding structure is arranged on the bracket and is used for adding ethanol with different concentrations into the reagent tubes; the feeding structure comprises a plurality of ethanol infusion tubes with different concentrations arranged on the bracket side by side and control valves fixedly mounted on the ethanol infusion tubes. Through the design of the feeding structure, ethanol solutions with different concentrations can be accurately added into the reagent tube, and different requirements in the sample dehydration process are met. Compared with a traditional method, by accurately controlling the adding amount of the ethanol, the consumption of the ethanol is remarkably reduced, and the experiment cost is reduced. Through the design of the swing structure, the mixing speed of ethanol and water in a sample is increased, and the dehydration time is shortened.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of biological sample dehydration devices, and particularly relates to a biological electron microscope sample dehydration device. BACKGROUND

[0002] In biological research and scanning electron microscope analysis, dehydration treatment of biological samples is a crucial step, especially for samples such as plant leaves, the internal water of which needs to be effectively removed before analysis. Traditional dehydration methods usually rely on a step-by-step dehydration process of ethanol solution, and the sample is immersed in ethanol solutions of different concentrations (such as 30%, 50%, 70%, 90% and 100% concentrations) to gradually replace and remove the internal water of the sample.

[0003] A biological electron microscope sample automatic dehydration device is disclosed in Chinese Utility Model Patent No. CN219307993U. The device realizes the automatic reciprocating movement of the sample in the dehydration tank by designing three groups of sliding rails and dehydration rack plates and using a disc driving mechanism, thereby improving the efficiency of dehydration treatment. This design simplifies the dehydration process to some extent, enabling plant leaves and other samples to be more uniformly immersed in ethanol solution.

[0004] However, in actual application, the device still has some deficiencies. First, because the space of the dehydration tank is relatively large, a large amount of ethanol solution is needed to fully immerse the sample and ensure effective mixing of ethanol and internal water of the sample. This not only increases the experimental cost but also may cause certain burden to the environment. Second, because the space of the dehydration tank is large, the mixing time of ethanol solution and internal water of the sample is also prolonged. This not only affects the efficiency of dehydration treatment but also may adversely affect the structure and integrity of the sample.

[0005] Therefore, the application provides a biological electron microscope sample dehydration device to solve the above problems. CONTENT OF THE UTILITY MODEL

[0006] The application provides a biological electron microscope sample dehydration device, which aims to solve the problems in the background art, such as the need for a large amount of ethanol solution to fully immerse the sample and ensure effective mixing of ethanol and internal water of the sample due to the relatively large space of the dehydration tank, which increases the experimental cost and may cause certain burden to the environment.

[0007] To achieve the above-mentioned purpose, the application provides the following technical solution: a biological electron microscope sample dehydration device, comprising a support, a positioning sleeve moving axially on the support, a reagent tube inserted into the positioning sleeve, and a swinging structure for driving the reagent tube to swing to accelerate the mixing of water and ethanol.

[0008] The application further comprises a feeding structure arranged on the support for adding different concentrations of ethanol into the reagent tube, which comprises a plurality of ethanol infusion tubes of different concentrations arranged side by side on the support and control valves fixedly installed on the ethanol infusion tubes. The biological sample to be dehydrated is placed in the reagent tube, and then the reagent tube is inserted into the positioning sleeve. When adding the ethanol solution into the interior of the reagent tube, the reagent tube is first moved below the ethanol infusion tube, then the control valve is opened to allow the liquid in the ethanol infusion tube to drop into the interior of the reagent tube, and then the control valve is closed. Then the swing structure is started to drive the reagent tube to swing in the positioning sleeve to accelerate the mixing of ethanol and water in the sample. After a period of time of swing mixing, the sample completes the dehydration treatment and can be subjected to subsequent experimental analysis. The swing structure accelerates the mixing speed of ethanol and water in the sample, shortens the dehydration time and improves the dehydration efficiency. By accurately controlling the ethanol concentration and the dehydration time, the application can better protect the integrity and structure of the sample and improve the accuracy of scanning electron microscope analysis.

[0009] Preferably, in order to ensure that the reagent tube is stably placed in the interior of the positioning sleeve, a silica gel sleeve is fixedly inserted into the interior of the positioning sleeve, and the reagent tube is movably inserted into the interior of the silica gel sleeve. The elasticity of the silica gel sleeve can stably place the reagent tube in the interior of the positioning sleeve, and can also reduce the wear of the positioning sleeve on the reagent tube.

[0010] Preferably, the swing structure comprises a drive motor, a cam fixedly connected to the output end of the drive motor, and a base fixedly connected to the lower end of the positioning sleeve and fixedly connected with the cam, and the lower end of the base abuts against the upper end of the drive motor. After the ethanol loading is completed, the drive motor drives the cam, the base, the positioning sleeve and the reagent tube to rotate, which can accelerate the mixing of water and ethanol in the reagent tube and shorten the mixing time of ethanol and water.

[0011] Preferably, in order to facilitate the axial movement of the reagent tube on the support, a guide rail is fixedly arranged on the support, and a sliding block for fixedly connecting with the lower end of the drive motor is axially movably sleeved on the guide rail. Through the cooperation of the sliding block and the guide rail, the swing structure is conveniently driven to move axially on the support, thereby facilitating the reagent tube to correspond to the ethanol infusion tubes of different concentrations.

[0012] Preferably, in order to facilitate accurate control of the reagent tube corresponding to the different concentrations of ethanol infusion tube: the support is provided with positioning grooves corresponding to different concentrations of the ethanol infusion tube, the inside of the sliding block is provided with a limiting groove, the inside of the limiting groove is slidably connected with a T-shaped insertion rod for inserting and cooperating with the positioning groove, and the limiting groove and the end of the T-shaped insertion rod away from the positioning groove are provided with a first spring. Wherein, the bottom end of the T-shaped insertion rod is spherical, and the shape of the inner wall of the positioning groove is consistent with the shape of the outer wall of the T-shaped insertion rod; the T-shaped insertion rod can be stably inserted in the positioning groove through the elastic force of the first spring, preventing displacement of the sliding block on the guide rail when the reagent tube is shaken.

[0013] Preferably, in order to prevent the liquid inside the ethanol infusion tube from dropping to the outside of the reagent tube: a lifting plate capable of lifting is movably arranged on the support, the ethanol infusion tube is fixedly installed on the lifting plate, and a second spring is arranged between the lower ends of the lifting plate on the support. The lifting plate facilitates the downward movement of the ethanol infusion tube, and then facilitates the insertion of the output end of the ethanol infusion tube into the inside of the reagent tube, avoiding the liquid from dropping to the outside of the reagent tube when dropping into the inside of the reagent tube, and causing the phenomenon of liquid waste.

[0014] The present application can accurately add ethanol solution of different concentrations into the reagent tube through the design of the feeding structure, meeting different needs in the sample dehydration process. Compared with the traditional method, the consumption of ethanol is significantly reduced by accurately controlling the amount of ethanol added, and the experimental cost is reduced. The mixing speed of ethanol and water in the sample is accelerated by the design of the swinging structure, the dehydration time is shortened, and the dehydration efficiency is improved. By accurately controlling the ethanol concentration and the dehydration time, the present application can better protect the integrity and structure of the sample and improve the accuracy of scanning electron microscope analysis. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structural schematic view of a biological electron microscope sample dehydration device;

[0016] Figure 2 It is a structural bottom view of Figure 1 ;

[0017] Figure 3 It is a structural front view of Figure 1 ;

[0018] Figure 4 It is a structural schematic view of the inside of the sliding block.

[0019] In the figure:

[0020] 1, support; 11, positioning groove; 2, positioning sleeve; 21, silica gel sleeve; 3, reagent tube; 4, swing structure; 41, driving motor; 42, cam; 43, base; 5, feeding structure; 51, ethanol infusion tube; 52, control valve; 6, guide rail; 61, sliding block; 611, limiting groove; 612, T-shaped insertion rod; 613, first spring; 7, lifting plate; 71, second spring. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0022] The present embodiment provides a biological electron microscope sample dehydration device, as shown in the figure, which comprises a support 1, a positioning sleeve 2 moving axially on the support 1, a reagent tube 3 inserted into the positioning sleeve 2, and a swing structure 4 for driving the reagent tube 3 to swing to accelerate the mixing of water and ethanol. Figures 1-4

[0023] It also comprises a feeding structure 5 arranged on the support 1 for adding different concentrations of ethanol into the reagent tube 3, and the feeding structure 5 comprises a plurality of ethanol infusion tubes 51 of different concentrations arranged side by side on the support 1 and a control valve 52 fixedly installed on the ethanol infusion tube 51. Through the design of the feeding structure 5, different concentrations of ethanol solution can be accurately added into the reagent tube 3 to meet different needs in the sample dehydration process. Compared with the traditional method, the present application significantly reduces the consumption of ethanol and reduces the experimental cost by accurately controlling the amount of ethanol added. The biological sample to be dehydrated is placed in the reagent tube 3, and then the reagent tube 3 is inserted into the positioning sleeve 2. When adding ethanol solution into the reagent tube 3, first move the reagent tube 3 to below the ethanol infusion tube 51, then open the control valve 52 to make the liquid in the ethanol infusion tube 51 drop into the interior of the reagent tube 3, and then close the control valve 52. Then start the swing structure 4 to drive the reagent tube 3 to swing in the positioning sleeve 2 to accelerate the mixing of ethanol and water in the sample. After a period of time of swing mixing, the sample completes the dehydration treatment and can be subjected to subsequent experimental analysis. The design of the swing structure 4 accelerates the mixing speed of ethanol and water in the sample, shortens the dehydration time, and improves the dehydration efficiency. By accurately controlling the ethanol concentration and the dehydration time, the present application can better protect the integrity and structure of the sample and improve the accuracy of scanning electron microscope analysis.

[0024] ​In order to ensure that the reagent tube 3 is stably placed inside the positioning sleeve 2: the inside of the positioning sleeve 2 is fixedly inserted with a silica gel sleeve 21, and the reagent tube 3 is movably inserted inside the silica gel sleeve 21. The silica gel sleeve 21 is made of medical silica gel, and through the elasticity of the silica gel sleeve 21, the reagent tube 3 can be stably placed inside the positioning sleeve 2, and at the same time, the wear of the positioning sleeve 2 on the reagent tube 3 can be reduced. When the sample is dehydrated, only the reagent tube 3 needs to be directly inserted into the silica gel sleeve 21, which is simple to operate.

[0025] The swing structure 4 includes a driving motor 41, a cam 42 fixedly connected to the output end of the driving motor 41, and a base 43 fixedly connected to the lower end of the positioning sleeve 2 and fixedly connected with the cam 42, and the lower end of the base 43 abuts against the upper end of the driving motor 41. After the ethanol loading is completed, the driving motor 41 drives the cam 42, the base 43, the positioning sleeve 2 and the reagent tube 3 to rotate, which can accelerate the mixing of water and ethanol in the reagent tube 3 and shorten the mixing time of ethanol and water.

[0026] In order to facilitate the axial movement of the reagent tube 3 on the support 1: a guide rail 6 is fixedly arranged on the support 1, and a sliding block 61 for fixedly connecting with the lower end of the driving motor 41 is axially movably sleeved on the guide rail 6. Through the cooperation of the sliding block 61 and the guide rail 6, the swing structure 4 is conveniently driven to move axially on the support 1, and then the reagent tube 3 can be correspondingly matched with the ethanol infusion tube 51 of different concentrations. When it is needed to add ethanol of different concentrations into the reagent tube 3, only the sliding block 61 on the guide rail 6 is moved to drive the positioning sleeve 2 connected with the swing structure 4 to move, which is simple to operate.

[0027] For the convenience of accurate control of the reagent tube 3 and the different concentrations of ethanol infusion tube 51 corresponding: the bracket 1 corresponding to different concentrations of ethanol infusion tube 51 are provided with positioning groove 11, the inside of the sliding block 61 is provided with a limiting groove 611, the limiting groove 611 is slidably connected with the T plug rod 612 for inserting into the positioning groove 11, the limiting groove 611 and the T plug rod 612 away from the positioning groove 11 one end is provided with the first spring 613. Among them, the bottom of the T plug rod 612 is spherical, the shape of the inner wall of the positioning groove 11 is consistent with the shape of the outer wall of the T plug rod 612; through the elastic force of the first spring 613 can make the T plug rod 612 is stably inserted into the positioning groove 11, prevent the displacement of the sliding block 61 on the guide rail 6 when the reagent tube 3 swing. When the reagent tube 3 needs to be moved to the next ethanol infusion tube 51 below, by hand sliding block 61, then pull the sliding block 61 along the guide rail 6, under the action of the sliding block 61 and the positioning groove 11 make the top of the T plug rod 612 to the inside of the limiting groove 611, the process of moving extrusion first spring 613 makes it shrink and store power, at the same time make the lower end of the T plug rod 612 moves out of the inside of the positioning groove 11, under the action of the first spring 613, make the lower end of the T plug rod 612 along the surface of the bracket 1 slide, until the lower end of the T plug rod 612 corresponds to another positioning groove 11, at this time under the action of the first spring 613 make the T plug rod 612 is inserted into the inside of the positioning groove 11 again, complete the position fixing of the sliding block 61, at this time the axis of the reagent tube 3 is just the same vertical line with the axis of the ethanol infusion tube 51.

[0028] In order to prevent the liquid in the ethanol infusion tube 51 from falling to the outside of the reagent tube 3: the bracket 1 is movably provided with a liftable lifting plate 7, the ethanol infusion tube 51 is fixedly installed on the lifting plate 7, and the second spring 71 is arranged between the lower end of the lifting plate 7. Through the lifting plate 7, the ethanol infusion tube 51 is conveniently lowered, and then the output end of the ethanol infusion tube 51 is inserted into the inside of the reagent tube 3, so that the liquid is not dropped to the outside of the reagent tube 3 when dropping into the inside of the reagent tube 3, and the waste of liquid is avoided. The second spring 71 is reset to facilitate the reset of the lifting plate 7, and the ethanol infusion tube 51 is separated from the reagent tube 3. When the ethanol liquid is dropped into the inside of the reagent tube 3, the lifting plate 7 is first moved downward by hand, so that the lifting plate 7 drives the ethanol infusion tube 51 to move downward, the second spring 71 is extruded in the process of moving the lifting plate 7 downward, so that it shrinks and stores power, until the output end of the ethanol infusion tube 51 is inserted into the inside of the reagent tube 3, the control valve 52 is opened, the liquid in the ethanol infusion tube 51 is dropped into the inside of the reagent tube 3, and then the control valve 52 is closed. Then the lifting plate 7 is reset under the action of the second spring 71, so that the output end of the ethanol infusion tube 51 moves out of the inside of the reagent tube 3.

[0029] The above merely provides the preferred embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art, according to the technical solution and concept of the present application, can make equivalent replacements or changes within the technical scope disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A biological electron microscope sample dehydrator apparatus, characterized by: The utility model provides a kind of ethanol-water mixing device, including support (1), the positioning sleeve (2) of axial movement on the support (1), reagent tube (3) is inserted in the positioning sleeve (2) and the swing structure (4) for driving the swing of reagent tube (3) to accelerate water and ethanol mixing; It also includes a feeding structure (5) disposed on the support (1) for adding different concentrations of ethanol into the reagent tube (3), the feeding structure (5) includes several ethanol infusion tubes (51) of different concentrations arranged side by side on the support (1) and control valves (52) fixedly installed on the ethanol infusion tubes (51).

2. The biological electron mirror sample dehydrator of claim 1, wherein: The inside of the positioning sleeve (2) is fixedly inserted with a silica gel sleeve (21), and the reagent tube (3) is movably inserted into the inside of the silica gel sleeve (21).

3. The biological electron microscope sample dehydrator of claim 1, wherein: The swing structure (4) includes a drive motor (41), a cam (42) fixedly connected to the output end of the drive motor (41), and a base (43) fixedly connected to the lower end of the positioning sleeve (2) and fixedly connected with the cam (42), and the lower end of the base (43) abuts against the upper end of the drive motor (41).

4. The biological electron microscope sample dehydrator of claim 1, wherein: The support (1) is fixedly provided with a guide rail (6), and the guide rail (6) is axially movably sleeved with a sliding block (61) for fixedly connecting with the lower end of the drive motor (41).

5. The biological electron microscope sample dehydrator of claim 4, wherein: The support (1) is provided with a positioning groove (11) corresponding to the ethanol infusion tube (51) of different concentrations, a limiting groove (611) is formed in the inside of the sliding block (61), a T-shaped insertion rod (612) is slidably inserted into the inside of the limiting groove (611) for insertion and cooperation with the positioning groove (11), and a first spring (613) is arranged at the end of the T-shaped insertion rod (612) away from the positioning groove (11).

6. The biological electron microscope sample dehydrator of claim 1, wherein: The support (1) is movably provided with a liftable lifting plate (7), the ethanol infusion tube (51) is fixedly installed on the lifting plate (7), and a second spring (71) is arranged between the lower ends of the lifting plate (7) corresponding to the support (1).

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