Separation device for biological sample extraction

Abstract

The utility model relates to the technical field of biomedical instruments, in particular to a separating device for extracting biological samples, which comprises a sample storage tube, a push plug and a small-diameter separator, the sample storage tube is provided with an operating end and a sampling end, the push plug is connected with a push rod and is slidably arranged in the sample storage tube through the push rod, and the small-diameter separator is arranged in the sample storage tube. The push rod is arranged on the side, close to the operation end, of the push plug, the small-diameter separator is connected with the sampling end of the sample storage tube and comprises an outer tube and a reducing inner tube, Luer tapers are arranged at the two ends of the outer tube, the inner tube is arranged in the outer tube, and the two ends of the inner tube are communicated with the Luer tapers at the two ends of the outer tube respectively. The Luer taper at one end of the outer tube is connected with the sampling end of the sample storage tube, and the Luer taper at the other end is provided with a plugging head. The separation device is a disposable device integrating sampling, centrifugation and trace extraction, reduces the hemolysis rate and the pollution rate, improves the separation precision and simplifies the operation process through the structure optimization and accurate control technology, and is suitable for clinical and laboratory complex sample treatment.

Description

TECHNICAL FIELD

[0001] The utility model relates to biomedical instrument technical field especially is involved in a kind of separation device for biological sample extraction. BACKGROUND

[0002] In the biomedical field, separating SVF (stromal vascular fraction), PRP (platelet-rich plasma), stem cells and other target components from biological samples such as blood and adipose tissue homogenate is crucial for clinical treatment and medical research. Traditional centrifugal separation method requires multiple transfers of biological samples to special centrifuge tubes. This process not only has complicated operation steps, significantly increasing the workload of medical staff or laboratory personnel, but also greatly increases the risk of sample hemolysis. According to the research of Chinese Journal of Laboratory Medicine, the probability of hemolysis caused by each operation during sample transfer can reach 10%-15%. Once hemolysis occurs, it will seriously affect the integrity and activity of target components in the sample, thereby interfering with subsequent analysis and treatment applications. At the same time, frequent sample transfer operations can easily cause cross-contamination, which may lead to deviations in test results and even mislead clinical diagnosis and treatment decisions. SUMMARY

[0003] To solve the problem of multiple transfers, complicated operation and cross-contamination in existing biological sample extraction, the utility model provides a separation device for biological sample extraction, which is a disposable device integrating sampling, centrifugation and micro-extraction. Through structural optimization and precise control technology, it reduces hemolysis rate and contamination rate, improves separation accuracy, simplifies operation process and is suitable for complex sample processing in clinical and laboratory.

[0004] The utility model provides a kind of separation device for biological sample extraction, including sample storage tube, push plug and fine diameter separator, sample storage tube has one operation end and one sampling end, push plug is connected with push rod and is slidably arranged in sample storage tube by push rod, push rod is arranged at the side of push plug close to operation end, fine diameter separator is connected with the sampling end of sample storage tube. The sample is first drawn into the sample storage tube from the sampling end by the cooperation of the push rod and the push plug, and then the fine diameter separator is installed at the sampling end. The sample in the tube is pushed into the fine diameter separator by the push plug, which facilitates high-precision separation of the sample.

[0005] Further, the fine diameter separator includes an outer tube and a variable-diameter inner tube. The outer tube has luer connectors at both ends. The inner tube is arranged inside the outer tube, and the luer connectors at both ends of the inner tube communicate with the luer connectors at both ends of the outer tube. The luer connector at one end of the outer tube is connected with the sampling end of the sample storage tube. The variable-diameter inner tube can more intuitively display the layered cells in the sample storage tube, facilitating more precise and micro sampling.

[0006] Further, the inner tube with variable diameter is vertically arranged in the outer tube, and the diameter of the inner tube decreases from the end close to the sample storage tube to the end away from the sample storage tube.

[0007] Further, the operation end of the sample storage tube is open and detachably provided with wing plates, and the wing plates are provided in two and symmetrically arranged on the sample storage tube.

[0008] Further, the operation end of the sample storage tube is open and detachably provided with a safety lock plate, and the bottom of the safety lock plate is hemispherical.

[0009] Further, the push plug comprises a rubber plug for sealing and a plastic plug for fixing, the push rod is connected with the plastic plug, one end of the rubber plug is connected with the plastic plug, and the other end is arranged in an arc shape, and the sampling end of the sample storage tube is arranged in an arc shape matched with the plastic plug.

[0010] Further, the auxiliary pushing support and the screw rod are further included, the sample storage tube is arranged on the auxiliary pushing support, the screw rod is screw-connected on the auxiliary pushing support, and one end of the screw rod is abutted with the push rod of the operation end of the sample storage tube.

[0011] Further, the auxiliary pushing support is provided with an upper fixed plate and a lower fixed plate, the upper fixed plate is provided with a positioning groove for accommodating the sample storage tube, the lower fixed plate is provided with a threaded hole matched with the screw rod, one end of the screw rod abutted with the push rod is provided with a push plate, and the other end is provided with a knob.

[0012] The beneficial effects of the utility model lie in:

[0013] The utility model provides a separation device for biological sample extraction, through detachable cooperation of the sample storage tube, the thin-diameter separator, the composite push plug and the auxiliary pushing support and the screw rod, the optimization of the structure is realized, sampling and centrifugation are completed in the same pipe body, the transfer of samples is reduced, sampling, centrifugation and micro-extraction are integrated, hemolysis rate and pollution rate can be effectively reduced, separation precision is improved, operation is reduced and efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0014] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the following will briefly introduce the drawings needed to be used in the specific embodiments or the prior art description. Obviously, the drawings described below are some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0015] Figure 1 is the external structure diagram of the separation device;

[0016] Figure 2 is the internal structure diagram of the separation device;

[0017] Figure 3 is the installation schematic diagram of the safety lock plate;

[0018] Figure 4 is the separation step schematic diagram;

[0019] In the figure, 1. sample storage tube, 2. push plug, 21. rubber plug, 22. plastic plug, 3. fine diameter separator, 31. outer tube, 32. inner tube, 33. luer joint, 34. plugging head, 4. push rod, 5. wing plate, 6. safety lock plate, 7. auxiliary support for pushing, 71. upper fixed plate, 72. lower fixed plate, 8. screw rod, 81. push plate, 82. knob. DETAILED DESCRIPTION

[0020] The technical solutions of the present application will be described clearly and completely in combination with the drawings. Obviously, the described embodiments are some embodiments of the present application, not all embodiments.

[0021] In order to reduce the transfer in the process of sample centrifugal separation, and to accurately extract the sample after centrifugation, a separation device for biological sample extraction is designed, as shown in Figure 1 The sample storage tube 1 has an operation end and a sampling end, the push plug 2 is connected with the push rod 4 and is slidingly arranged in the sample storage tube 1 through the push rod 4, the push rod 4 is arranged on the side of the push plug 2 close to the operation end, and the fine diameter separator 3 is connected with the sampling end of the sample storage tube 1.

[0022] The material of the sample storage tube 1 is medical-grade polyethylene terephthalate, polypropylene, and can withstand centrifugal force ≥4000G. A push plug 2 is slidably arranged in the sample storage tube 1 by a push rod 4. The sample storage tube 1, the push plug 2 and the push rod 4 form a syringe structure. The sampling end of the sample storage tube 1 is provided with a luer joint 33, which can be screwed with a needle tube. The push rod 4 drives the push plug 2 to move towards the operation end, so that the needle tube at the sampling end is used to draw the biological sample into the sample storage tube 1. Then the push rod 4 is removed, and the sample storage tube 1 containing the biological sample is placed in a centrifuge for centrifugation operation, so that the biological sample is accurately separated into SVF (stromal vascular fraction), PRP (platelet-rich plasma), stem cells and other target components distributed in layers. Then the push rod 4 is screwed onto the push plug 2, and a fine separator 3 is screwed onto the luer joint 33 at the sampling end. The push rod 4 drives the push plug 2 to move towards the sampling end, so that the layered components in the sample storage tube 1 are sequentially extruded from the sampling end into the fine separator 3. The fine separator 3 is made of transparent medical plastic material, which can more directly display each component. In addition, the layered components are difficult to extract due to their large volume, and the components of adjacent layers are also easy to extract. The volume on each horizontal surface of the fine separator 3 is smaller, which is more convenient for accurate extraction, and meets the efficient processing needs of multi-level samples. The sample storage tube 1 integrates sampling, centrifugation and micro-layer extraction functions. The sample does not need to be transferred multiple times, which reduces the hemolysis rate and the pollution rate, improves the separation accuracy, simplifies the operation process, and is suitable for complex sample processing in clinics and laboratories.

[0023] When the biological sample is sampled, a wing plate 5 can also be installed on the operation end of the sample storage tube 1. The wing plate 5 is provided with two wing plates 5, which are symmetrically arranged on the sample storage tube 1 by a mounting ring. The operation end of the sample storage tube 1 is provided with an L-shaped groove for clamping the mounting ring. An external thread can also be arranged on the L-shaped groove. The L-shaped groove with an external thread is used to screw an insurance lock piece 6 or a mounting ring. The wing plate 5 is convenient for pushing and pulling the push rod 4, and is more convenient for sampling. After sampling is completed, the wing plate 5 and the needle tube at the sampling end are removed, and a plug head 34 is installed on the luer joint 33 at the sampling end to prevent sample leakage and prepare for subsequent centrifugation operation. The plug head 34 is designed with a composite diaphragm, such as silicone and polypropylene, which supports vacuum pre-setting (negative pressure 30-50kPa). After sampling by puncturing the plug head 34 with a needle tube, it can be self-sealed to reduce bubble interference.

[0024] In order to make the whole centrifugal device more beautiful, the wing plate 5 and the insurance lock piece 6 can be arranged in an embedded structure, that is, the outer surfaces of the mounting rings of the insurance lock piece 6 and the wing plate 5 are flush with the outer surface of the sample storage tube 1. The installation method is preferably a rotating buckle structure, and a threaded connection can also be used.

[0025] For example, Figure 3As shown, when the sample storage tube 1 containing the biological sample is placed in the centrifuge for centrifugation, a safety locking piece 6 is installed at the operating end of the sample storage tube 1. The bottom of the safety locking piece 6 is hemispherical, fitting the hemispherical groove of the centrifuge chamber. The safety locking piece 6 is used to fix the position of the push stopper 2 inside the tube, preventing liquid leakage caused by displacement of the push stopper 2 during centrifugation. The sample storage tube 1 is then placed in the centrifuge, and appropriate centrifugation parameters are set to separate the biological sample.

[0026] To enable precise and minute extraction of the separated target components, a propulsion support 7 and a screw 8 are also included. The sample storage tube 1 is placed on the propulsion support 7, and the screw 8 is screwed onto the propulsion support 7. The screw 8 abuts against the push rod 4 at the operating end of the sample storage tube 1. A wing plate 5 is installed at the operating end of the sample storage tube 1. The wing plate 5 restricts the relative position of the sample storage tube 1 and the propulsion support 7, so that the screw 8 is aligned with the push rod 4. The displacement accuracy of the push plug 2 can be controlled by controlling the thread advance of the screw 8 (accuracy ±0.1mm).

[0027] The manual push rod 4 and screw 8 can be switched freely to assist in injection, balancing ease of operation with high precision requirements.

[0028] like Figure 2 As shown, specifically, the fine-diameter separator 3 includes an outer tube 31 and a variable-diameter inner tube 32. Both ends of the outer tube 31 are equipped with Luer connectors 33. The inner tube 32 is located inside the outer tube 31, with both ends connected to the Luer connectors 33 at both ends of the outer tube 31. One end of the outer tube 31 is connected to the sampling end of the sample storage tube 1. The variable-diameter inner tube 32 is vertically positioned inside the outer tube 31, with its diameter decreasing from the end closer to the sample storage tube 1 to the end further away. The layered components in the sample storage tube 1 are squeezed out of the sample storage tube 1 by the pusher 2 and into the fine-diameter separator 3. The fine-diameter separator 3 has the smallest diameter and volume at its top, facilitating more precise and minute extraction of the target components. Furthermore, the smooth inner wall of the inner tube 32 reduces liquid adsorption, ensuring no loss of trace components (such as stem cell layers and platelet layers).

[0029] To better push the sample into the sample storage tube 1, the pusher 2 includes a rubber stopper 21 for sealing and a plastic stopper 22 for fixing. The pusher 4 is connected to the plastic stopper 22. One end of the rubber stopper 21 is connected to the plastic stopper 22, and the other end is set in an arc shape. The sampling end of the sample storage tube 1 is set in an arc shape that matches the plastic stopper 22. The rubber stopper 21 is made of butyl rubber and has an arc-shaped groove on the liquid contact surface to guide the target precipitate to gather. To avoid liquid residue in the tube, the sampling end of the sample storage tube 1 is also set in an arc shape that matches the arc-shaped groove. The rubber stopper 21 fits the inner wall of the tube without any dead angles to ensure zero residue. The plastic stopper 22 is made of medical-grade plastic (PP or PETG) and has a threaded hole in the center of the bottom. The threaded hole is used to screw the pusher 4 into the end of the pusher 4 to form a syringe-type injection structure.

[0030] In order to accurately push the components, the pushing auxiliary support 7 has an upper fixed plate 71 and a lower fixed plate 72, the upper fixed plate 71 is provided with a positioning groove capable of accommodating the sample storage tube 1, the lower fixed plate 72 is provided with a threaded hole matched with the screw rod 8, one end of the screw rod 8 abutting with the push rod 4 is provided with a push plate 81, and the other end is provided with a knob 82. The screw rod 8 is controlled to move upwards by rotating the knob 82, and the push rod 4 is driven to move upwards, that is, the push plug 2 is controlled to move upwards to discharge (the accuracy is ±0.1mm), and the micro-layer extraction is realized in cooperation with the fine diameter separator 3.

[0031] As shown in Figure 4 , the use steps of the separation device are as follows:

[0032] Step one: assemble: screw in the push rod 4, connect the push rod 4 and the push plug 2 together, then install the wing plate 5, so that the sample storage tube 1, the wing plate 5, the push rod 4 and the push plug 2 form a push injection type syringe structure;

[0033] Step two: sampling: connect a needle on the luer connector 33 at the sampling end of the sample storage tube 1, pull the push rod 4 towards the operation end to extract the fat homogenate into the sample storage tube 1;

[0034] Step three: sealing: remove the needle and install the plug 34;

[0035] Step four: centrifugal preparation: remove the wing plate 5 and install the safety lock plate 6, so that the sample storage tube 1 and the safety lock plate 6 form a centrifugal tube;

[0036] Step five: centrifugation: place the sample storage tube 1 into the centrifugal groove of the main flow centrifuge, centrifuge at 1000G for 10 minutes, and separate out the SVF layer;

[0037] Step six: accurate extraction: place the centrifuged sample storage tube 1 in the positioning groove of the upper fixed plate 71 of the pushing auxiliary support 7, the end of the push rod 4 in the sample storage tube 1 abuts against the push plate 81 on the lower fixed plate 72, the tube body is fixed, the screw rod 8 is controlled to push the push plug 2 by rotating the knob 82, the liquid in the tube is pushed into the fine diameter separator 3, and the target layer is accurately extracted from the fine diameter separator 3;

[0038] Step seven: subsequent processing: take out the target layer and apply it to different scenes as needed.

[0039] The separation device integrates sampling, centrifugation and micro-extraction functions, realizes residue-free and high-precision layered operation, is suitable for mainstream centrifuges, significantly reduces hemolysis rate, pollution rate and operation time, is suitable for clinical blood, fat tissue SVF extraction and stem cell separation, and in the extraction process, through detachable design of the sample storage tube 1, the fine separator 3, the plugging head 34 and the safety lock plate 6, the structure is optimized, so that the biological sample is in the sample storage tube 1 during sampling and centrifugation, the sample transfer is reduced, the sampling and centrifugal separation are completed in one step, the operation is reduced, and the efficiency is improved.

[0040] The above description is only illustrative in nature and is not intended to limit the present application, and those skilled in the art understand that many modifications, changes or equivalents can be made without departing from the spirit and scope defined by the appended claims.

Claims

1. A separation device for biological sample extraction, characterized by: The application relates to a sample storage tube, which comprises a sample storage tube (1), a push plug (2) and a fine-diameter separator (3), the sample storage tube (1) has an operation end and a sampling end, the push plug (2) is connected with a push rod (4) and is slidably arranged in the sample storage tube (1) through the push rod (4), the push rod (4) is arranged at one side of the push plug (2) close to the operation end, and the fine-diameter separator (3) is connected with the sampling end of the sample storage tube (1). The fine-diameter separator (3) comprises an outer tube (31) and a variable-diameter inner tube (32), a luer connector (33) at one end of the outer tube (31) is connected with the sampling end of the sample storage tube (1). The operation end of the sample storage tube (1) is open and detachably provided with a wing plate (5). The operation end of the sample storage tube (1) is open and detachably provided with a safety lock plate (6).

2. The separation device for biological sample extraction according to claim 1, characterized in that: The outer tube (31) is provided with the luer connector (33) at both ends, the inner tube (32) is arranged in the outer tube (31), and the inner tube (32) is in communication with the luer connectors (33) at both ends of the outer tube (31) respectively.

3. The separation device for biological sample extraction according to claim 2, wherein: The variable-diameter inner tube (32) is vertically arranged in the outer tube (31), and the diameter of the inner tube (32) decreases from the end close to the sample storage tube (1) to the end far from the sample storage tube (1).

4. The separation device for biological sample extraction according to claim 1, wherein: The wing plate (5) is provided with two wing plates (5) which are symmetrically arranged on the sample storage tube (1).

5. The separation device for biological sample extraction of claim 1, wherein: The safety lock plate (6) is hemispherical at the bottom.

6. The separation device for biological sample extraction of claim 1, wherein: The push plug (2) comprises a rubber plug (21) for sealing and a plastic plug (22) for fixing, the push rod (4) is connected with the plastic plug (22), one end of the rubber plug (21) is connected with the plastic plug (22), and the other end is arranged in an arc shape, and the sampling end of the sample storage tube (1) is arranged in an arc shape matched with the plastic plug (22).

7. The separation device for biological sample extraction of claim 1, wherein: The application further comprises a propelling auxiliary support (7) and a screw rod (8), the sample storage tube (1) is arranged on the propelling auxiliary support (7), the screw rod (8) is screw-connected on the propelling auxiliary support (7), and the screw rod (8) is in abutment with the push rod (4) of the operation end of the sample storage tube (1).

8. The separation device for biological sample extraction according to claim 7, characterized in that: The propelling auxiliary support (7) has an upper fixed plate (71) and a lower fixed plate (72), the upper fixed plate (71) is provided with a positioning groove capable of accommodating the sample storage tube (1), the lower fixed plate (72) is provided with a threaded hole matched with the screw rod (8), one end of the screw rod (8) in abutment with the push rod (4) is provided with a push plate (81), and the other end is provided with a knob (82).

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