Nucleic acid extraction equipment

Abstract

The utility model discloses nucleic acid extraction equipment, which comprises a support, a horizontal moving mechanism, a rotating mechanism, a heating assembly and a vertical moving mechanism, the horizontal moving mechanism comprises a first sliding seat and a first driving piece, the first sliding seat is arranged on the support and can slide along the horizontal direction, and the rotating mechanism comprises a bracket and a second driving piece. The bracket is rotationally connected with the first sliding seat and used for bearing consumables, the heating assembly is arranged on the bracket and used for heating the consumables, the vertical moving mechanism comprises a third sliding seat and a fourth sliding seat, the third sliding seat and the fourth sliding seat can slide in the vertical direction, the third sliding seat is connected with a third driving part, and the fourth sliding seat is connected with a fourth driving part. The third sliding seat is arranged on the magnetic bar, the fourth sliding seat is arranged on the sheath, the third driving part is used for driving the third sliding seat to slide in the vertical direction so that the magnetic bar can be inserted into or withdrawn from the sheath, the fourth driving part is used for driving the sheath to enter or withdraw from the consumable, and the extraction purity of nucleic acid can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid extraction purification technical field, especially in kind of nucleic acid extraction equipment. BACKGROUND

[0002] The mainstream technology of existing nucleic acid extraction is magnetic bead method, the magnetic bead method is to use nanometer magnetic beads to combine with nucleic acid molecules specifically, and under the action of an external magnetic field, the magnetic beads can be adsorbed by a magnet, so that the characteristics of separating nucleic acid from biological samples are achieved, so as to complete the purpose of nucleic acid extraction and purification. When the magnetic bead method is extracted, mainly including sample lysis, nucleic acid washing, nucleic acid purification and nucleic acid recovery steps, wherein each step needs to use a magnetic rod sheath to mix the sample, the magnetic rod and the magnetic rod sheath are combined to adsorb the magnetic beads and transfer the magnetic beads, and the magnetic rod and the magnetic rod sheath are separated to realize the transfer of the magnetic beads to the target position. The existing nucleic acid extraction equipment of the magnetic bead method has low concentration and purity of the extracted trace nucleic acid sample, which reduces the accuracy of nucleic acid detection. SUMMARY

[0003] The utility model aims at at least one of the technical problems existing in the prior art. Therefore, the utility model provides a nucleic acid extraction equipment, which can improve the extraction purity and concentration of nucleic acid.

[0004] The nucleic acid extraction equipment according to the embodiment of the utility model, comprising:

[0005] A support;

[0006] The horizontal moving mechanism comprises a first sliding seat and a first driving part, the first sliding seat is arranged on the support and can slide in the horizontal direction, and the first driving part is used to drive the first sliding seat to slide in the horizontal direction;

[0007] The rotating mechanism comprises a bracket and a second driving part, the bracket is rotationally connected with the first sliding seat, the second driving part is used to drive the bracket to rotate, and the bracket is used to carry consumables;

[0008] A heating assembly is arranged on the bracket, and the heating assembly is used to heat the consumables;

[0009] The vertical moving mechanism comprises a third sliding seat and a fourth sliding seat, the third sliding seat and the fourth sliding seat can slide in the vertical direction, the third sliding seat is connected with a third driving part, the fourth sliding seat is connected with a fourth driving part, the third sliding seat is arranged on a magnetic rod, the fourth sliding seat is arranged on a sheath, the third driving part is used to drive the third sliding seat to slide in the vertical direction, so that the magnetic rod is inserted into or withdrawn from the sheath, and the fourth driving part is used to drive the sheath to enter or exit the consumables.

[0010] The nucleic acid extraction device has at least the following beneficial effects:

[0011] When lysis is performed, the fourth driving member drives the sheath into the consumable, the first driving member drives the first sliding base to slide in the horizontal direction, and the second driving member drives the bracket to rotate, and the lysis reagent is mixed and homogenized by combining the high-frequency vibration in the up-down direction of the sheath and the high-speed rotating movement of the consumable; meanwhile, the heating assembly can heat the consumable, so that the cell membrane of the cells in the consumable is destroyed, the cells lose integrity, and the nucleic acid in the cells is released; after lysis is completed, the third driving member drives the magnetic rod into the sheath to extract the nucleic acid; by arranging the heating assembly, heating can dissociate the nucleic acid from other cell components such as proteins and lipids, remove impurities, and improve the extraction purity and concentration of the nucleic acid.

[0012] According to some embodiments of the present application, the nucleic acid extraction device is provided with a first transmission structure, and the first driving member drives the first sliding base to slide in the horizontal direction through the first transmission structure.

[0013] According to some embodiments of the present application, the first transmission structure comprises a first transmission wheel, a second transmission wheel, a first fixing member and a first transmission belt, the first transmission wheel is arranged on the support, the second transmission wheel is arranged on the output end of the first driving member, the first transmission belt is arranged around the first transmission wheel and the second transmission wheel, and the first fixing member is arranged on the first sliding base and fixedly connected with the first transmission belt.

[0014] According to some embodiments of the present application, the support is provided with a first guide rail, the first sliding base is provided with a first guide block, the first guide block is provided with a first guide groove, and the first guide rail penetrates through the first guide groove and abuts against the side wall of the first guide groove.

[0015] According to some embodiments of the present application, the heating assembly is located on the side of the bracket facing the first sliding base, and the heating assembly is a heating wire, a heating sheet or a heating rod.

[0016] According to some embodiments of the present application, the output end of the second driving member is provided with a first eccentric wheel, the axis of the first eccentric wheel is eccentrically arranged with the axis of the output end of the second driving member, and the first eccentric wheel is connected with the bracket.

[0017] According to some embodiments of the present application, a support structure is arranged between the bracket and the first sliding base, and the first sliding base supports the bracket through the support structure.

[0018] According to some embodiments of the present application, the support structure comprises a support shaft rotatably arranged on the first sliding seat, one end of the support shaft is provided with a second eccentric wheel, and the second eccentric wheel is in abutment with the bracket.

[0019] According to some embodiments of the present application, the bracket is provided with a groove on one side close to the first sliding seat, and the second eccentric wheel is contained in the groove and in abutment with the side wall of the groove.

[0020] According to some embodiments of the present application, a plurality of support shafts are arranged, the plurality of support shafts are arranged along the axis of the second driving member at intervals, and the second eccentric wheel corresponds to the support shaft one by one.

[0021] The additional aspects and advantages of the present application will be partially given in the following description, partially will become obvious from the following description, or will be understood through the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0022] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings in which:

[0023] Figure 1 It is a structural schematic view of the nucleic acid extraction equipment of the embodiment of the present application;

[0024] Figure 2 It is a front view of the horizontal moving mechanism of the embodiment of the present application;

[0025] Figure 3 It is a structural schematic view of the horizontal moving mechanism of the embodiment of the present application, wherein the bracket and the consumables are hidden;

[0026] Figure 4 It is an assembly schematic view of the bracket and the consumables of the embodiment of the present application;

[0027] Figure 5 It is an assembly schematic view of the bracket and the consumables of the embodiment of the present application from another perspective;

[0028] Figure 6 It is a structural schematic view of the vertical moving mechanism of the embodiment of the present application;

[0029] Figure 7 It is a structural schematic view of the vertical moving mechanism of the embodiment of the present application from another perspective.

[0030] REFERENCE NUMERALS:

[0031] Support 100, bottom plate 101, vertical plate 102, first guide rail 110;

[0032] The first sliding seat 210, the supporting shaft 211, the second eccentric wheel 212, the first driving piece 220, the first transmission wheel 231, the second transmission wheel 232, the first fixing piece 233, the transmission belt 234, the first guide block 240;

[0033] The second driving piece 310, the first eccentric wheel 311, the bracket 320, the recess 321;

[0034] The third sliding seat 410, the fourth sliding seat 420, the third driving piece 430, the fourth driving piece 440, the magnetic rod 450, the sheath 460, the consumable 500. DETAILED DESCRIPTION

[0035] The embodiments of the present application are described in detail below, examples of which are shown in the drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary, only for explaining the present application, and cannot be understood as a limitation of the present application.

[0036] In the description of the present application, it is understood that the orientation description, such as the orientation or position relationship indicated by up, down, front, back, left, right, etc. is based on the orientation or position relationship shown in the drawings, only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore cannot be understood as a limitation of the present application.

[0037] In the description of the present application, the meaning of several is one or more, the meaning of multiple is more than two, greater than, less than, more than, etc. is not included in the number, above, below, etc. is understood to include the number. If it is described to the first, the second is only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the order of indicated technical features.

[0038] In the description of the present application, unless otherwise explicitly limited, the words such as setting, installing, connecting, etc. should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application according to the specific content of the technical scheme.

[0039] In the related art, the mainstream technology of existing nucleic acid extraction is the magnetic bead method. The magnetic bead method uses the characteristics that nanometer magnetic beads can specifically bind to nucleic acid molecules and can be adsorbed by a magnet under the action of an external magnetic field, so as to separate nucleic acid from a biological sample, thereby achieving the purpose of nucleic acid extraction and purification. When the magnetic bead method is used for extraction, mainly includes sample lysis, nucleic acid washing, nucleic acid purification and nucleic acid recovery and other steps, wherein each step needs to use a magnetic rod sheath to mix the sample, the magnetic rod and the magnetic rod sheath are combined to adsorb the magnetic beads and transfer the magnetic beads, and the magnetic rod and the magnetic rod sheath are separated to transfer the magnetic beads to the target position. The existing nucleic acid extraction equipment of the magnetic bead method has low concentration and purity of the extracted trace nucleic acid sample, which reduces the accuracy of nucleic acid detection.

[0040] Reference Figures 1 to 7 According to the nucleic acid extraction equipment of the first aspect of the present application, the nucleic acid extraction equipment comprises a support 100, a horizontal moving mechanism, a rotating mechanism, a heating assembly and a vertical moving mechanism. The horizontal moving mechanism comprises a first sliding seat 210 and a first driving member 220. The first sliding seat 210 is arranged on the support 100 and can slide in the horizontal direction. The first driving member 220 is used to drive the first sliding seat 210 to slide in the horizontal direction. The rotating mechanism comprises a bracket 320 and a second driving member 310. The bracket 320 is rotationally connected with the first sliding seat 210. The second driving member 310 is used to drive the bracket 320 to rotate. The bracket 320 is used to carry consumables 500. The heating assembly is arranged on the bracket 320. The heating assembly is used to heat the consumables 500. The vertical moving mechanism comprises a third sliding seat 410 and a fourth sliding seat 420. Both the third sliding seat 410 and the fourth sliding seat 420 can slide in the vertical direction. The third sliding seat 410 is connected with a third driving member 430. The fourth sliding seat 420 is connected with a fourth driving member 440. The third sliding seat 410 is arranged on a magnetic rod 450. The fourth sliding seat 420 is arranged on a sheath 460. The third driving member 430 is used to drive the third sliding seat 410 to slide in the vertical direction, so that the magnetic rod 450 is inserted into or withdrawn from the sheath 460. The fourth driving member 440 is used to drive the sheath 460 to enter or exit the consumables 500. On the one hand, by arranging the horizontal moving mechanism, the rotating mechanism and the vertical moving mechanism, when lysis is performed, the mixing and lysis reagent are realized by combining the high-frequency vibration of the sheath 460 in the upward and downward directions and the high-speed rotary motion of the consumables 500. On the other hand, the heating assembly can heat the consumables 500, so that the cell membrane of the cells in the consumables 500 is destroyed, the cells lose integrity, and the nucleic acid in the cells is released. Heating can also dissociate the nucleic acid from other cell components such as proteins and lipids, remove impurities, and improve the extraction purity of the nucleic acid.

[0041] For example, when lysis is performed, the fourth driving member 440 drives the sheath 460 into the consumable 500, the first driving member 220 drives the first sliding base 210 to slide in the horizontal direction, the second driving member 310 drives the bracket 320 to rotate, and the lysis reagent is mixed and combined by combining the high-frequency vibration of the sheath 460 in the vertical direction and the high-speed rotating movement of the consumable 500. Meanwhile, the heating assembly can heat the consumable 500, so that the cell membrane of the cell in the consumable 500 is destroyed, the cell loses integrity, and the nucleic acid in the cell is released. After lysis is completed, the third driving member 430 drives the magnetic rod 450 into the sheath 460 to extract the nucleic acid. By arranging the heating assembly, heating can dissociate the nucleic acid from other cell components such as proteins, lipids, etc., remove impurities, and improve the extraction purity of the nucleic acid.

[0042] It should be noted that the support 100 comprises a bottom plate 101 and a vertical plate 102 connected to the bottom plate 101, the bottom plate 101 and the vertical plate 102 are arranged vertically, the bottom plate 101 is arranged in the horizontal direction, and the vertical plate 102 is arranged in the vertical direction, the first sliding base 210 is slidably arranged on the bottom plate 101, and the third sliding base 410 and the fourth sliding base 420 are arranged on the vertical plate 102, which is not limited herein.

[0043] In some embodiments of the utility model, the nucleic acid extraction equipment is provided with a first transmission structure, the first driving member 220 drives the first sliding base 210 to slide in the horizontal direction through the first transmission structure, and the moving stability of the first sliding base 210 can be improved.

[0044] In some embodiments of the utility model, the first transmission structure comprises a first transmission wheel 231, a second transmission wheel 232, a first fixing member 233 and a first transmission belt 234, the first transmission wheel 231 is arranged on the support 100, the second transmission wheel 232 is arranged on the output end of the first driving member 220, the first transmission belt 234 is arranged around the first transmission wheel 231 and the second transmission wheel 232, and the first fixing member 233 is arranged on the first sliding base 210 and is fixedly connected with the first transmission belt 234, so that the moving stability of the first sliding base 210 can be improved.

[0045] As another implementation, the first transmission structure can also be a gear and rack transmission structure, the gear and rack transmission structure comprises a rack arranged on the first sliding base 210 and a gear arranged on the output end of the first driving member 220, the gear is engaged with the rack, and the moving stability of the first sliding base 210 can also be improved.

[0046] In some embodiments of the utility model, the support 100 is provided with the first guide rail 110, the first sliding seat 210 is provided with the first guide block 240, the first guide block 240 is provided with the first guide groove, the first guide rail 110 is arranged in the first guide groove and abuts against the side wall of the first guide groove, the first sliding seat 210 can be guided to slide along the preset direction, thereby improving the moving stability of the first sliding seat 210.

[0047] It should be noted that the positions of the first guide rail 110 and the first guide block 240 can also be interchanged, that is, the first guide rail 110 is arranged on the first sliding seat 210, and the first guide block 240 is arranged on the support 100, which is not limited herein.

[0048] In some embodiments of the utility model, the heating assembly is a heating wire, the heating wire is located on one side of the bracket 320 close to the first sliding seat 210, when the heating wire works, the heat generated by the heating wire is transmitted to the consumable 500 through the bracket 320, so that the temperature of the reagent in the consumable 500 is raised, the cell membrane rupture of the cell can be accelerated, and the extraction purity of the nucleic acid is improved.

[0049] It should be noted that the heating assembly can also be a heating element such as a heating sheet or a heating rod, which is not limited herein.

[0050] In some embodiments of the utility model, the output end of the second driving member 310 is provided with the first eccentric wheel 311, the axis of the first eccentric wheel 311 is eccentrically arranged with the axis of the output end of the second driving member 310, the first eccentric wheel 311 is connected with the bracket 320, so that the bracket 320 can realize eccentric rotation, and the cell lysis effect can be improved.

[0051] For example, the axis of the first eccentric wheel 311 is arranged in parallel with the axis of the output end of the second driving member 310, and the axis of the first eccentric wheel 311 is not collinear with the axis of the output end of the second driving member 310, when the second driving member 310 drives the first eccentric wheel 311 to rotate, the first eccentric wheel 311 rotates eccentrically relative to the second driving member 310, thereby driving the reagent in the consumable 500 to rotate at high speed, and the cell lysis effect can be improved.

[0052] In some embodiments of the utility model, the bracket 320 and the first sliding seat 210 are provided with a support structure, the first sliding seat 210 supports the bracket 320 through the support structure, the bracket 320 can be supported to reduce the bending deformation of the bracket 320, thereby improving the service life of the bracket 320.

[0053] In some embodiments of the utility model, support structure includes the support shaft 211 of rotating to be located in first sliding seat 210, support shaft 211 is equipped with second eccentric wheel 212 near one end of bracket 320, second eccentric wheel 212 and bracket 320 abut, can support bracket 320, to reduce the bending deformation of bracket 320, thereby improve the service life of bracket 320.

[0054] For example, by setting second eccentric wheel 212, make the support position of second eccentric wheel 212 and bracket 320 unchanged, the friction between second eccentric wheel 212 and bracket 320 is static friction, can reduce the friction between second eccentric wheel 212 and bracket 320, on the one hand, can reduce the wear of bracket 320, on the other hand, second eccentric wheel 212 can support bracket 320, to reduce the bending deformation of bracket 320, thereby improve the service life of bracket 320.

[0055] In some embodiments of the utility model, the side of bracket 320 near first sliding seat 210 is equipped with groove 321, second eccentric wheel 212 is contained in groove 321 and abuts with the side wall of groove 321, can be circumferentially positioned second eccentric wheel 212, to facilitate the installation of second eccentric wheel 212.

[0056] It should be noted that the bearing is arranged between second eccentric wheel 212 and support shaft 211, which can reduce the rotating resistance of bracket 320, so that bracket 320 can rotate smoothly, which is not limited here.

[0057] In some embodiments of the utility model, support shaft 211 is configured with multiple, multiple support shafts 211 are arranged along the axis of second driving part 310, and multiple second eccentric wheels 212 are correspondingly arranged, which can support bracket 320 in all directions, thereby reducing the bending deformation of bracket 320 and improving the service life of bracket 320.

[0058] For example, bracket 320 is of rectangular structure, and support shaft 211 is configured with four, four support shafts 211 are respectively located at the corresponding corners of bracket 320, which can support bracket 320 in all directions, thereby reducing the bending deformation of bracket 320 and improving the service life of bracket 320.

[0059] The technical features of the above-described embodiments can be combined arbitrarily, and to make the description concise, not all possible combinations of the technical features in the above-described embodiments are described, however, as long as the combinations of the technical features do not exist contradictory, it should be considered that it is within the scope of the present application.

[0060] The above embodiment is described in detail in combination with the drawings, but the utility model is not limited to the above embodiment, and various changes can be made within the knowledge range possessed by those skilled in the art in the technical field without departing from the purpose.

Claims

1. A nucleic acid extraction device, characterized in that, include: Bracket (100); The horizontal moving mechanism includes a first slide (210) and a first driving member (220). The first slide (210) is disposed on the bracket (100) and can slide in the horizontal direction. The first driving member (220) is used to drive the first slide (210) to slide in the horizontal direction. The rotating mechanism includes a bracket (320) and a second drive member (310). The bracket (320) is rotatably connected to the first slide (210). The second drive member (310) is used to drive the bracket (320) to rotate. The bracket (320) is used to carry consumables (500). A heating assembly is provided on the bracket (320), and the heating assembly is used to heat the consumable (500); The vertical moving mechanism includes a third slide (410) and a fourth slide (420), both of which can slide vertically. The third slide (410) is connected to a third driving member (430), and the fourth slide (420) is connected to a fourth driving member (440). The third slide (410) is located on a magnetic rod (450), and the fourth slide (420) is located on a protective sleeve (460). The third driving member (430) is used to drive the third slide (410) to slide vertically so that the magnetic rod (450) can be inserted into or removed from the protective sleeve (460). The fourth driving member (440) is used to drive the protective sleeve (460) to enter or exit the consumable (500).

2. The nucleic acid extraction device according to claim 1, characterized in that, The nucleic acid extraction device is provided with a first transmission structure, and the first driving member (220) drives the first slide (210) to slide in the horizontal direction through the first transmission structure.

3. The nucleic acid extraction device according to claim 2, characterized in that, The first transmission structure includes a first transmission wheel (231), a second transmission wheel (232), a first fixing member (233), and a first transmission belt (234). The first transmission wheel (231) is disposed on the bracket (100), the second transmission wheel (232) is disposed on the output end of the first driving member (220), the first transmission belt (234) is wound around the first transmission wheel (231) and the second transmission wheel (232), and the first fixing member (233) is disposed on the first slide (210) and fixedly connected to the first transmission belt (234).

4. The nucleic acid extraction device according to claim 1, characterized in that, The bracket (100) is provided with a first guide rail (110), the first slide (210) is provided with a first guide block (240), the first guide block (240) is provided with a first guide groove, and the first guide rail (110) passes through the first guide groove and abuts against the side wall of the first guide groove.

5. The nucleic acid extraction device according to claim 1, characterized in that, The heating component is located on the side of the bracket (320) facing the first slide (210), and the heating component is a heating wire, a heating plate or a heating rod.

6. The nucleic acid extraction device according to claim 1, characterized in that, The output end of the second drive member (310) is provided with a first eccentric wheel (311), the axis of the first eccentric wheel (311) is eccentrically set with the axis of the output end of the second drive member (310), and the first eccentric wheel (311) is connected to the bracket (320).

7. The nucleic acid extraction device according to claim 6, characterized in that, A support structure is provided between the bracket (320) and the first slide (210), and the first slide (210) supports the bracket (320) through the support structure.

8. The nucleic acid extraction device according to claim 7, characterized in that, The support structure includes a support shaft (211) rotatably mounted on the first slide (210), and a second eccentric wheel (212) is provided at one end of the support shaft (211) near the bracket (320), and the second eccentric wheel (212) abuts against the bracket (320).

9. The nucleic acid extraction device according to claim 8, characterized in that, The bracket (320) has a groove (321) on the side near the first slide (210), and the second eccentric wheel (212) is housed in the groove (321) and abuts against the side wall of the groove (321).

10. The nucleic acid extraction device according to claim 8, characterized in that, Multiple support shafts (211) are provided, and the multiple support shafts (211) are spaced apart along the axis of the second drive member (310). The second eccentric wheel (212) corresponds to each of the support shafts (211).

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