Rare earth ion doped luminescent nanoprobe
By setting a double-layer protective structure and a detachable end on the rare-earth ion-doped luminescent nanoprobe, the problem of probe breakage is solved, and the durability and economy of the probe are improved, making it suitable for frequent use in university laboratories.
Patent Information
- Application Number
- CN202520421525.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-12
- Publication Date
- 2026-02-24
- Estimated Expiration
- 2035-03-12
AI Technical Summary
Rare earth ion-doped luminescent nanoprobes are small and easily broken, which can lead to damage, affect detection progress, and increase costs. They are also expensive and lack durability, which is a heavy economic burden, especially when used frequently in university laboratories.
The nanoprobe is designed with a dual-layer protective structure, including a probe body and first and second ends, with anti-corrosion and wear-resistant layers respectively, to enhance the durability of the probe. The ends are also interchangeable to extend the service life.
The double-layer protective structure and detachable end design significantly enhance the durability of the probe, extend its service life, reduce the frequency of damage and replacement, and reduce the economic burden.
Smart Images
Figure CN223940791U_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of nanoprobe technology, specifically a rare-earth ion-doped luminescent nanoprobe. Background Technology
[0002] Rare-earth ion-doped luminescent nanoprobes utilize near-infrared lasers as the light source, avoiding the damage to cells or tissues caused by ultraviolet light excitation. They offer advantages such as good photochemical stability, low biotoxicity, long fluorescence lifetime, narrow emission peak, and large Stokes shift. Furthermore, they enable precise identification of cancer cells, solving the problem of damaging or even inducing carcinogenesis in ordinary cells during cancer treatment. Therefore, rare-earth ion-doped luminescent nanoprobes have attracted considerable attention from experts and scholars, becoming a research hotspot.
[0003] Rare-earth ion-doped luminescent nanoprobes possess advantages such as small size, high precision, and strong performance, making them highly popular in the biological and medical fields. However, to minimize the traumatic experience during biological detection, rare-earth ion-doped luminescent nanoprobes are generally designed to be as small as possible. However, excessively small nanoprobes are prone to breakage, leading to damage that not only affects the detection process but can also delay treatment and worsen the condition, increasing detection costs. Therefore, the hardness of the nanoprobes needs to be optimized for application. Furthermore, rare-earth ion-doped luminescent nanoprobes are relatively expensive. Breakage, deformation, or frequent replacements can create a significant financial burden, especially in university laboratories where experiments are conducted frequently and the number of users is large, easily leading to budget shortages. Therefore, improving the reproducibility and durability of rare-earth ion-doped luminescent nanoprobes is particularly crucial.
[0004] Therefore, based on the existing technology, this utility model designs a probe structure with a double-layer protective structure, combined with a fixing layer and two detachable ends, aiming to enhance the durability of rare earth ion-doped luminescent nanoprobes, extend their service life, and thus enhance their application performance, especially in repeated, stable and sustainable experiments in university laboratories. Utility Model Content
[0005] The purpose of this invention is to provide a rare-earth ion-doped luminescent nanoprobe that is simple in structure, easy to operate, and practical and durable, so as to solve the problems mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution:
[0007] A rare-earth ion-doped luminescent nanoprobe includes a probe body with a first end and a second end detachably connected to its two ends. Rare-earth ions, such as La, are doped into the probe body, the first end, and the second end.3+ Er 3+ or Pr 3+ One of them;
[0008] The outer side of the probe body is sequentially connected to a first anti-corrosion layer, a first fixing layer, an insulating layer and a first wear-resistant layer, and the first fixing layer has a structure that is thick in the middle and thin at both sides;
[0009] The outer side of the first end is provided with a second anti-corrosion layer, the outer side of the second anti-corrosion layer is covered with a second wear-resistant layer, the second wear-resistant layer is provided with a second fixing layer, and the second fixing layer is fixed on the second anti-corrosion layer; the side of the first end away from the probe body is covered with a biocompatible layer, and the biocompatible layer is connected to the second anti-corrosion layer;
[0010] The structure of the second end is the same as that of the first end.
[0011] Preferably, the first end and the second end are symmetrically arranged about the probe body.
[0012] Preferably, the thickness of the first anti-corrosion layer and the second anti-corrosion layer is 1-2 nm, and the thickness of the first wear-resistant layer is 2-3 nm.
[0013] Preferably, the thickness of the biocompatible layer is not greater than the thickness of the second anti-corrosion layer.
[0014] Preferably, the thickness of the first fixing layer is 0.5-1.5 μm, and the thickness of the edge of the first fixing layer does not exceed half the thickness of the middle of the first fixing layer.
[0015] Preferably, the second fixing layer corresponds to the first fixing layer, and the thickness of the second fixing layer is not greater than the thickness of the edge of the first fixing layer.
[0016] Preferably, the length of the second fixing layer is less than the length of the second anti-corrosion layer.
[0017] Preferably, the thickness of the second wear-resistant layer is greater than the thickness of the first wear-resistant layer, and the thickness of the second wear-resistant layer is not greater than the sum of the thicknesses of the first fixing layer, the insulating layer, and the first wear-resistant layer. This is because the first and second ends of the second wear-resistant layer are the parts that directly contact and rub against the substance being tested. The setting of this part can enhance the wear resistance of the first and second ends and enhance the durability of the probe.
[0018] Preferably, the side of the second wear-resistant layer away from the probe body has an arc structure.
[0019] Preferably, the second wear-resistant layer has fixing holes.
[0020] Compared with the prior art, the beneficial effects of this utility model are:
[0021] This invention creatively provides a double-layer protective structure with an anti-corrosion layer and a wear-resistant layer on the probe body, the first end, and the second end, respectively. The anti-corrosion layer provides chemical protection to the probe, preventing it from being oxidized or corroded by acids and alkalis, while the wear-resistant layer provides physical wear protection to prevent the probe from being damaged by friction due to repeated use. This double-layer protective structure further enhances the durability of the probe and extends its service life.
[0022] This invention creatively provides a first end and a second end with identical structures that can be detachably connected. In use, the first end and the second end serve as the detection end and the connection end, respectively. When the first end is worn down due to long-term use as the detection end, affecting its use, the first end and the second end can be reversed, so that the original first end serves as the connection end and the original connection end serves as the detection end, thus extending the service life of the probe. When both the first end and the second end are worn down due to long-term use, affecting their use, the first end and the second end can be disassembled and replaced, facilitating maintenance.
[0023] This invention creatively incorporates a first fixing layer and a second fixing layer, which ensures that the probe body, the first end, and the second end have a certain degree of rigidity while reducing the probe size. This not only reduces the wound surface of the probe during biological detection but also prevents it from being broken or deformed during use, thereby enhancing the durability of the probe and facilitating its promotion and application. Attached Figure Description
[0024] Figure 1 This is a schematic diagram of the main structure of a rare earth ion-doped luminescent nanoprobe according to the present invention.
[0025] Figure 2 This is an enlarged structural diagram of part A in this utility model;
[0026] In the figure, 1-probe body, 2-first end, 3-second end, 4-first anti-corrosion layer, 5-first fixing layer, 6-insulating layer, 7-first wear-resistant layer, 8-second anti-corrosion layer, 9-second fixing layer, 10-second wear-resistant layer, 11-biocompatible layer, 12-fixing hole. Detailed Implementation
[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0028] Please see Figure 1 and Figure 2 This utility model provides: Specific Implementation Example 1
[0030] A rare earth ion-doped luminescent nanoprobe includes a probe body 1, with a first end 2 and a second end 3 detachably connected to its two ends (the probe body, the first end, and the second end are doped with rare earth ions, which resonate when they sense incident light, resulting in a surface plasmon effect, thereby achieving a response to light of different wavelengths and combining with corresponding functional molecules to form a fluorescent probe with specific recognition functions).
[0031] In this embodiment 1, a 1nm thick first anti-corrosion layer 4, a first fixing layer 5, and a 2nm thick first wear-resistant layer 7 are sequentially connected to the outside of the probe body 1. The first fixing layer 5 has a structure that is thick in the middle and thin at both ends. The thickness of the first fixing layer 5 is 0.5μm, and the thickness of the edge of the first fixing layer 5 is less than half the thickness of the middle of the first fixing layer 5, forming a "convex" shape. Without changing the strength enhancement performance, the material usage can be reduced, thereby saving costs (the insulating layer 6 covers the first fixing layer 5, forming an inverted concave shape).
[0032] In this embodiment 1, a second anti-corrosion layer 8 with a thickness of 1 nm is provided on the outer side of the first end 2. The outer side of the second anti-corrosion layer 8 is covered by a second wear-resistant layer 10. The thickness of the second wear-resistant layer 10 is greater than the thickness of the first wear-resistant layer 7, and the thickness of the second wear-resistant layer 10 is equal to the sum of the thicknesses of the first fixing layer 5, the insulating layer 6, and the first wear-resistant layer 7. In this embodiment 1, the side of the second wear-resistant layer 10 away from the probe body 1 is an arc structure to reduce the resistance during the probe insertion process. A second fixing layer 9 is provided inside the second wear-resistant layer 10 and is fixed to the second anti-corrosion layer 8. The length of the second fixing layer 9 is less than the length of the second anti-corrosion layer 8, so as to increase the probe end length without affecting the probe end detection process. The second fixing layer 9 corresponds to the first fixing layer 5, and the thickness of the second fixing layer 9 is equal to the thickness of the edge of the first fixing layer 5. In this embodiment 1, a biocompatible layer 11 (such as a chitosan layer) connected to the second anti-corrosion layer 8 is wrapped around the side of the first end 2 away from the probe body 1, and its thickness is equal to the thickness of the second anti-corrosion layer 8, which is beneficial to improving the biocompatibility of the nanoprobe and promoting the probe to perform detection work. In addition, an elongated oval fixing hole 12 is provided in the second wear-resistant layer 10. The fixing hole 12 is parallel to the probe body 1, and the opening of the fixing hole 12 faces the side away from the probe body 1, which is beneficial to fixing the probe. The design of the elongated oval structure is beneficial to enhancing the stability of the probe connection.
[0033] In this embodiment 1, the structure of the second end 3 is the same as that of the first end 2, and the first end 2 and the second end 3 are symmetrically arranged about the probe body 1. Specific Implementation Example 2
[0035] In this embodiment 2, the rare earth ion-doped luminescent nanoprobe has the same structure as in embodiment 1, with the following differences: the thickness of the first anti-corrosion layer 4 is set to 2 nm, the thickness of the first wear-resistant layer 7 is set to 3 nm, the thickness of the first fixing layer 5 is 1.5 μm, and the thickness of the edge of the first fixing layer 5 is equal to half the thickness of the middle of the first fixing layer 5; the thickness of the second anti-corrosion layer 8 is set to 2 nm, and the thickness of the second wear-resistant layer 10 is set to be less than the sum of the thicknesses of the first fixing layer 5, the insulating layer 6, and the first wear-resistant layer 7; the thickness of the second fixing layer 9 is less than the thickness of the edge of the first fixing layer 5. Specific Implementation Example 3
[0037] In this embodiment 3, the rare earth ion-doped luminescent nanoprobe has the same structure as in embodiment 1, with the following differences: the thickness of the first anti-corrosion layer 4 is 1.5 nm, the thickness of the first wear-resistant layer 7 is 2.3 nm, the thickness of the first fixing layer 5 is 1.0 μm, and the thickness of the edge of the first fixing layer 5 is equal to half the thickness of the middle of the first fixing layer 5; the thickness of the second anti-corrosion layer 8 is 1.6 nm; and the thickness of the biocompatible layer 11 is less than the thickness of the second anti-corrosion layer 8.
[0038] In use, the first end of this invention is designated as the detection end for probe detection, while the second end serves as the connection end for fixing to a fixed component, specifically through a fixing hole. The first anti-corrosion layer on the probe body prevents damage, and the first fixing layer on top of this layer increases the probe's hardness. Furthermore, the insulating layer and the first wear-resistant layer on its surface enhance the probe's durability (the dual-layer protection structure of the first anti-corrosion layer and the first wear-resistant layer extends the probe's lifespan). The biocompatible layer on the first end, serving as the detection end, improves the probe's biocompatibility, facilitating interaction between the probe and organisms for detection. The second wear-resistant layer on the surface of the first end prevents damage to the detection end, enhancing its durability. The second anti-corrosion layer (located within the second wear-resistant layer) protects the first end from damage even if the second wear-resistant layer is damaged (the dual-layer protection structure of the second anti-corrosion layer and the second wear-resistant layer extends the probe's lifespan).
[0039] The second end of this invention has the same structure as the first end and is symmetrically arranged, so that the second end and the first end can serve as both a detection end and a connection end. That is, when the first end is worn down due to long-term use as a detection end, affecting its use, the first end and the second end can be reversed, so that the original first end serves as the connection end and the original connection end serves as the detection end, thus extending the service life of the probe. When both the first end and the second end are worn down due to long-term use, affecting their use, the first end and the second end can be disassembled and replaced, facilitating maintenance and further extending the service life of the probe.
[0040] As can be seen from the above embodiments of this utility model, this utility model has a double-layer protective structure with an anti-corrosion layer and a wear-resistant layer. Through different mechanisms of action, it achieves physical and chemical protection, which significantly enhances the service life of the probe. It also increases the hardness of the probe, preventing it from being broken or bent during use. The detachable ends of the probe body can not only serve as both detection and connection ends (i.e., when the first end is used as the detection end, the second end is used as the connection end accordingly. When the first end is worn after long-term use, the first end can be used as the connection end, while the second end is used as the detection end accordingly, which enhances the durability of the probe), but also facilitates disassembly, replacement and maintenance.
[0041] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A rare-earth ion-doped luminescent nanoprobe, characterized in that: It includes a probe body, with a first end and a second end detachably connected to both ends of the probe body; The outer side of the probe body is sequentially connected to a first anti-corrosion layer, a first fixing layer, an insulating layer and a first wear-resistant layer, and the first fixing layer has a structure that is thick in the middle and thin at both sides; The outer side of the first end is provided with a second anti-corrosion layer, the outer side of the second anti-corrosion layer is covered with a second wear-resistant layer, the second wear-resistant layer is provided with a second fixing layer, and the second fixing layer is fixed on the second anti-corrosion layer; the side of the first end away from the probe body is covered with a biocompatible layer, and the biocompatible layer is connected to the second anti-corrosion layer; The structure of the second end is the same as that of the first end.
2. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The first end and the second end are symmetrically arranged about the probe body.
3. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The thickness of the first and second anti-corrosion layers is 1-2 nm, and the thickness of the first wear-resistant layer is 2-3 nm.
4. The rare-earth ion-doped luminescent nanoprobe according to claim 1 or 3, characterized in that: The thickness of the biocompatible layer is no greater than the thickness of the second anti-corrosion layer.
5. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The thickness of the first fixing layer is 0.5-1.5 μm, and the thickness of the edge of the first fixing layer does not exceed half the thickness of the middle of the first fixing layer.
6. The rare-earth ion-doped luminescent nanoprobe according to claim 5, characterized in that: The second fixing layer corresponds to the first fixing layer, and the thickness of the second fixing layer is not greater than the thickness of the edge of the first fixing layer.
7. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The length of the second fixing layer is less than the length of the second anti-corrosion layer.
8. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The thickness of the second wear-resistant layer is greater than the thickness of the first wear-resistant layer, and the thickness of the second wear-resistant layer is not greater than the sum of the thicknesses of the first fixing layer, the insulating layer, and the first wear-resistant layer.
9. The rare-earth ion-doped luminescent nanoprobe according to claim 1 or 8, characterized in that: The second wear-resistant layer has an arc-shaped structure on the side away from the probe body.
10. The rare-earth ion-doped luminescent nanoprobe according to claim 1, characterized in that: The second wear-resistant layer has fixing holes.