Magnetic bead cleaning system for chemiluminescence immunity analyzer

Abstract

The utility model discloses a magnetic bead cleaning system for a chemiluminescence immunoassay analyzer, which comprises a waste liquid container, a first container, a second container and a cleaning module, and the cleaning module comprises a plurality of extraction and injection needle groups, a solid-liquid separation needle, a liquid injection and cleaning unit and a liquid suction unit, each suction and injection needle group comprises a first liquid injection needle, an anti-overflow needle, a second liquid injection needle, a first suction needle and a second suction needle which are arranged in sequence, and the first suction needle, the second suction needle and the anti-overflow needle are connected with the liquid suction unit. The extraction and injection needle set is composed of five needles, two-injection and three-suction can be carried out, and the magnetic bead cleaning effect is ensured; besides, magnetic bead cleaning liquid is crystalline cleaning liquid, suction needles in the suction and injection needle sets have the risk of blockage, once blockage occurs, the cleaning liquid overflows to generate crystals, an anti-overflow needle is additionally arranged in each suction and injection needle set, and when the liquid level in the reaction cup exceeds a certain height, the excessive liquid can be pumped away through the anti-overflow needles. And liquid in the reaction cup is prevented from overflowing.

Description

TECHNICAL FIELD

[0001] The utility model relates to the liquid path field of in-vitro diagnosis equipment, especially to a magnetic bead cleaning system for a chemiluminescence immunoassay analyzer. BACKGROUND

[0002] The liquid path system is the core execution module for realizing full automation detection of the in-vitro diagnosis equipment. The liquid path system of the existing chemiluminescence analyzer mainly includes a sample adding needle / reagent needle module, a substrate module, a magnetic bead cleaning module, a sample adding needle and reagent needle cleaning module, a waste liquid treatment module, and the like. These modules are used to realize sample sampling / adding, reagent sampling / adding, substrate sampling / adding, magnetic bead cleaning liquid adding / waste liquid discharge, sample adding needle and reagent needle cleaning, cleaning mechanism waste liquid treatment, overall liquid path system, pipeline maintenance treatment, and the like. Among them, the magnetic bead cleaning module is a key module for determining the reliability of the detection result. The existing magnetic bead cleaning system mostly adopts a cleaning system that alternates one injection, one suction, one injection, and one suction in a cycle. However, the magnetic bead cleaning liquid is often a crystalline washing liquid. Once the suction needle fails during the working process, the liquid in the reaction cup will overflow, causing liquid leakage. SUMMARY

[0003] Therefore, the utility model provides a magnetic bead cleaning system for a chemiluminescence immunoassay analyzer.

[0004] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0005] The magnetic bead cleaning system for the chemiluminescence immunoassay analyzer includes a waste liquid container, a first container containing magnetic bead cleaning liquid, and a second container containing pipeline cleaning liquid. The system also includes at least one cleaning module for cleaning the magnetic beads in the reaction cup. The cleaning module includes multiple sets of injection and suction needle groups, a solid-liquid separation needle, a liquid injection and cleaning unit, and a liquid suction unit. Each set of injection and suction needle groups includes a first liquid injection needle, an anti-overflow needle, a second liquid injection needle, a first suction needle, and a second suction needle arranged in sequence.

[0006] The liquid injection and cleaning unit includes a liquid injection pump. The inlet side of the liquid injection pump is provided with a first valve. The inlet of the first valve has two branches. One branch is connected with the first container, and the other branch is connected with the second container through an internal washing pipeline. The internal washing pipeline is provided with a flushing pump. The outlet side of the liquid injection pump is provided with a second valve. The outlet of the second valve is connected with a first liquid distributor and a second liquid distributor in parallel. The first liquid distributor is connected with the first liquid injection needle through a first liquid injection pipeline. Each first liquid injection pipeline is provided with a third valve. The second liquid distributor is connected with the second liquid injection needle through a second liquid injection pipeline. Each second liquid injection pipeline is provided with a fourth valve.

[0007] The first suction needle, the second suction needle and the anti-overflow needle are connected with the liquid suction unit.

[0008] Compared with the prior art, the injection and suction needle group has five needles, can perform two injections and three suction, and ensures the magnetic bead cleaning effect; the magnetic bead cleaning liquid is usually a crystalline cleaning liquid, the suction needle in the injection and suction needle group has a risk of blockage, after blockage, normal suction cannot be performed, and excess liquid in the reaction cup is caused; when secondary injection and suction is performed, if direct continuous double injection is performed, the reaction cup overflows, the anti-overflow needle is arranged between the first liquid injection needle and the second liquid injection needle, excess liquid can be sucked away by the anti-overflow needle, and the overflow of liquid in the reaction cup is avoided. Furthermore, purified water can be pumped into the whole pipeline by the flushing pump, the first liquid injection needle and the second liquid injection needle are internally flushed, and crystallization blockage of the liquid injection needle is avoided as much as possible.

[0009] In the preferred embodiment of the utility model, the liquid suction unit includes a first suction pump, a second suction pump and an anti-overflow pump, all of which are communicated with the waste liquid container, the first suction needle is connected with the suction channel of the first suction pump through a first suction pipeline, and each second suction needle is connected with the suction channel of the second suction pump through a second suction pipeline.

[0010] The plurality of anti-overflow needles are connected with the anti-overflow pump through an anti-overflow pipeline, or at least one anti-overflow needle is connected with the first suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump, or at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump, or at least one anti-overflow needle is connected with the first suction pump, at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump.

[0011] The first suction pump and the second suction pump of each cleaning module are multi-channel pumps, the number of pumps is reduced, and the pipeline is simplified; in addition, the first suction needle and the second suction needle are connected with different suction pumps, and the blockage risk of the suction pump is reduced as much as possible.

[0012] In a more preferred embodiment of the utility model, at least one anti-overflow needle is connected with the first suction pump, at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump. The plurality of anti-overflow needles of the cleaning module are divided into three parts, are connected with the first suction pump, the second suction pump and the anti-overflow pump respectively, and the blockage probability of the anti-overflow needle is further reduced.

[0013] In a more preferred embodiment of the utility model, the first suction pump is connected with the waste liquid container through the first liquid collector, the second suction pump is connected with the waste liquid container through the second liquid collector, the solid-liquid separation needle is connected with the first liquid collector or the second liquid collector through a separation pipeline, and a separation pump is arranged on the separation pipeline. The beneficial effect is that after cleaning, the liquid in the reaction cup can be completely sucked out by the separation pump and the solid-liquid separation needle, the magnetic beads and the liquid are separated, and subsequent detection is facilitated.

[0014] Preferably, the outlet of the anti-overflow pump is connected with the waste liquid container through a third liquid collector, a liquid level sensor or a weight sensor is arranged between the third liquid collector and the first liquid collector or between the third liquid collector and the second liquid collector, when liquid is detected, it indicates that the anti-overflow needle has sucked the excess liquid, and then it indicates that the two first suction needles and / or the suction needle are blocked.

[0015] In a preferred embodiment of the utility model, the two branches on the inlet side of the first valve are connected through a two-position three-way electromagnetic valve. Of course, in actual connection, the two branches on the inlet side of the first valve can also be connected through a three-way joint, and an electromagnetic valve is respectively installed on each branch to control the on-off of each branch.

[0016] In a preferred embodiment of the utility model, the first liquid distributor is connected with the waste liquid container through a first exhaust pipeline, the second liquid distributor is connected with the waste liquid container through a second exhaust pipeline, a first exhaust valve is arranged on the first exhaust pipeline, and a second exhaust valve is arranged on the second exhaust pipeline. The beneficial effect is that during the cleaning process, the water in the liquid distributor is first discharged into the waste liquid container, and the bubbles in the pipeline are discharged.

[0017] In a preferred embodiment of the utility model, the magnetic bead cleaning system for the chemiluminescence immunoassay analyzer further comprises a first suction unit and a flushing unit, the first suction unit comprises a first pump, a sample adding needle connected in sequence through a pipeline, and a second pump and a reagent needle connected through a pipeline, the flushing unit comprises a flushing pipeline connected with the second container, a flushing pump is arranged on the flushing pipeline, and the first pump and the second pump are connected in parallel at the outlet of the flushing pump. The beneficial effect is that each cleaning module of the utility model is matched with a set of first suction unit and flushing unit, and the suction of the sample and the addition of the reagent can be carried out.

[0018] In an optimal embodiment of the utility model, each group of the cleaning module further comprises a first suction and cleaning unit, the first suction and cleaning unit comprises a first needle, a first pump, a fifth valve connected through a first pipeline and a second needle, a second pump and a sixth valve connected through a second pipeline, the first suction and cleaning unit further comprises a cleaning pool, a first cleaning pump, a second cleaning pump and a waste liquid pump, the inlet of the first cleaning pump and the inlet of the second cleaning pump are connected with the second container, the outlet of the first cleaning pump is divided into two ways and is connected with the first pump and the second pump respectively, the outlet of the second cleaning pump is connected with the inlet of the cleaning pool, the inlet of the waste liquid pump is connected with the outlet of the bottom of the cleaning pool, and the outlet of the waste liquid pump is connected with the waste liquid container. The beneficial effect is that the concentration of the mixture after the reaction of the sample, the magnetic beads and the reagent is usually large, the first needle and the second needle of the utility model can work alternately, the continuous first suction of multiple reaction cups on the reaction disc is realized, and the needs of the assembly line detection are met.

[0019] In a more optimal embodiment of the utility model, a pressure detection module is arranged between the first needle and the first pump and between the second needle and the second pump, and whether the two needles are blocked can be judged according to the pressure.

[0020] In an optimal embodiment of the utility model, the bottom opening height of the first liquid injection needle and the second liquid injection needle is consistent, the bottom opening height of the first suction needle is higher than that of the second suction needle, and the bottom opening of the anti-overflow needle is lower than that of the first liquid injection needle and higher than that of the first suction needle, so that the excess liquid can be extracted.

[0021] Compared with the prior art, the utility model has multiple suction and injection needle groups, the suction and injection needle group has five needles, two injections and three suction can be carried out, and the cleaning effect of the magnetic beads is ensured. In addition, the magnetic bead cleaning liquid is usually a crystalline washing liquid, the suction needle in the suction and injection needle group has a risk of blockage, and once blocked, the washing liquid will overflow to the reaction disc outside the reaction cup and produce crystalline substances. The utility model adds an anti-overflow needle in each suction and injection needle group, and when the liquid level in the reaction cup exceeds a certain height, the anti-overflow needle can extract the excess liquid, avoiding the overflow of the liquid in the reaction cup. Furthermore, purified water can be pumped into the entire pipeline by using the flushing pump to flush the inside of the first liquid injection needle and the second liquid injection needle, so as to avoid the crystalline blockage of the liquid injection needle as much as possible. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is a schematic diagram of the utility model.

[0023] Figure 2 It is a layout diagram of the suction and injection needle group (five groups) of the utility model.

[0024] Figure 3 It is a schematic diagram of the liquid injection and cleaning unit (two groups) of the utility model.

[0025] Figure 4 is a schematic view of the liquid suction unit (two groups) of the utility model.

[0026] Figure 5 is a schematic view of the first suction and cleaning unit (one group) of the utility model. DETAILED DESCRIPTION

[0027] The embodiment of the utility model will be described in detail below in combination with the drawings, and the embodiment is implemented on the premise of the technical scheme of the utility model, and detailed implementation mode and specific operation process are given, but the protection scope of the utility model is not limited to the following embodiment.

[0028] It should be noted that, in the description of the utility model, the relationship terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between the entities or operations.

[0029] In the description of the utility model, unless otherwise explicitly specified and limited, the terms "connected", "connected" that can appear should be understood broadly, for example, can be fixedly connected, or integrally connected, can be mechanically connected, or electrically connected, can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0030] As shown in Figures 1-5 The magnetic bead cleaning system for chemiluminescence immunoassay analyzer of the utility model, including waste liquid container 100, the first container 200 filled with magnetic bead cleaning liquid and the second container 300 filled with pipeline cleaning liquid (preferably purified water), two groups of cleaning modules for cleaning the magnetic beads in the reaction cup, and then high-throughput detection is realized, and the detection efficiency of high-throughput samples is improved. Wherein, each cleaning module includes a plurality of groups of injection needle group 400, solid-liquid separation needle 500, liquid injection and cleaning unit and liquid suction unit, each injection needle group 400 includes first liquid injection needle 401, anti-overflow needle 402, second liquid injection needle 403, first suction needle 404 and second suction pump 405 arranged in sequence, each injection needle group 400 can realize one injection, one suction, one injection and two suction, and the cleaning effect of the magnetic beads in the reaction cup is ensured; Wherein, the bottom opening height of first liquid injection needle 401 and second liquid injection 403 is consistent, the bottom opening height of first suction needle 404 is higher than that of second suction needle 405, and the bottom opening of anti-overflow needle 402 is lower than that of first liquid injection needle 401 and higher than that of first suction needle 404, so that the anti-overflow needle 402 can extract excess liquid after the needle seat is lowered in place;

[0031] The magnetic bead cleaning solution is a crystalline cleaning solution, and the suction needle in the suction and injection needle set 400 has a risk of blockage, which will cause the liquid in the reaction cup to overflow outward when the subsequent suction and injection needle set is injected. The utility model adds an anti-overflow needle 402 in each suction and injection needle set 400, when the liquid level of the reaction cup located directly below the anti-overflow needle 402 is higher than a certain height (that is, the suction needle of the upper suction and injection needle set is blocked), the anti-overflow needle can suck away the excess liquid in the reaction cup, avoiding the overflow of the reaction cup when the second liquid injection needle is injected.

[0032] In combination Figure 3 It can be known that the liquid injection and cleaning unit comprises a liquid injection pump P1, the inlet side of the liquid injection pump P1 is provided with a first valve D1, the inlet of the first valve D1 has two branches, one of which is connected with the first container 200, and the other is connected with the second container 300 through an internal washing pipeline, and the internal washing pipeline is provided with a flushing pump P2; the outlet side of the liquid injection pump P1 is provided with a second valve D2, the outlet of the second valve D2 is connected in parallel with a first distributor 601 and a second distributor 602, the first distributor 601 is connected with the first liquid injection needle 401 one by one through a first liquid injection pipeline 603, each first liquid injection pipeline 603 is provided with a third valve D3, and the second distributor 602 is connected with the second liquid injection needle 403 one by one through a second liquid injection pipeline 604, each second liquid injection pipeline 604 is provided with a fourth valve D4. In actual work, the liquid injection and cleaning unit of the utility model not only can realize quantitative injection of the magnetic bead cleaning solution, but also can realize internal cleaning of each liquid injection needle, avoiding blockage of the liquid injection pipeline.

[0033] In combination Figure 3 It can be known that the first distributor 601 is connected with the waste liquid container 100 through a first exhaust pipeline 605, the second distributor 602 is connected with the waste liquid container 100 through a second exhaust pipeline 606, the first exhaust pipeline 605 is provided with a first exhaust valve D7, and the second exhaust pipeline 606 is provided with a second exhaust valve D8. In the internal flushing process, the flushing pump P2 is opened, so that the entire pipeline is filled with purified water, so that the bubbles in the pipeline and each distributor are discharged. In addition, the liquid injection pump P1 of the utility model is injected into each liquid injection needle through the distributor, the distributor can balance the pipeline pressure, and then the high-precision injection of each branch is ensured. Since the pipeline flow resistance of each branch is different, the plurality of third valves D3 and the plurality of fourth valves D4 can be opened at intervals according to the pipeline flow resistance in actual work, so as to ensure that the liquid injection amounts of the plurality of first liquid injection needles 401 are consistent.

[0034] In actual installation, the two branches of the inlet side of the first valve D1 are connected by a two-position three-way electromagnetic valve 607, and the on-off state of each branch and the first valve D1 can be adjusted by the two-position three-way electromagnetic valve 607. Of course, the two branches and the inlet of the first valve D1 can also be connected together through a three-way joint, and an electromagnetic valve is installed on each branch to realize the separate control of each branch and meet the liquid injection and internal cleaning requirements.

[0035] In actual installation, the first suction needle 404, the second suction needle 405 and the anti-overflow needle 402 are connected with the liquid suction unit, and the liquid in the reaction cup is sucked out by the liquid suction unit, thereby ensuring the cleaning effect of the magnetic beads in each reaction cup and improving the reliability of the detection result. Figure 1 , 4 It can be seen that the liquid suction unit comprises a first suction pump P3, a second suction pump P4 and an anti-overflow pump P5, all of which are connected with the waste liquid container 100; the first suction needle 404 is connected with the suction channel of the first suction pump P3 through a first suction pipeline, and each second suction needle 405 is connected with the suction channel of the second suction pump P4 through a second suction pipeline; the first suction pump P3, the second suction pump P4 and the anti-overflow pump P5 all have multiple flow channels, which can meet the connection requirements of multiple suction needles and the anti-overflow needle 402, reduce the number of pumps, and thereby simplify the pipeline; in addition, each cleaning module has two matching suction pumps, which also reduces the failure rate of the pipeline to a certain extent.

[0036] In this embodiment, the anti-overflow needle 402 is connected with the first suction pump P3 and the second suction pump P4. Figure 2 and 4 It can be seen that each injection needle group 400 of the utility model has one anti-overflow needle 402 (which is actually also used for suction), and there are five anti-overflow needles 402. The installation modes of the anti-overflow needles of the two groups of magnetic cleaning modules are the same, and taking the five anti-overflow needles of one group of magnetic cleaning modules as an example: three anti-overflow needles 402 are connected with three channels of the anti-overflow pump P5 one by one through an anti-overflow pipeline, one anti-overflow needle 402 is connected with the first suction pump P3, and the other anti-overflow needle 402 is connected with the second suction pump P4, thereby installing the five anti-overflow needles 402 on different pumps, and thereby reducing the failure rate of the anti-overflow pipeline.

[0037] Of course, in actual installation, the number of anti-overflow needles 402 can also be six or more or four, three, etc. When the number of anti-overflow needles 402 is small, the anti-overflow needles 402 can be connected with two suction pumps respectively, or two or one anti-overflow needles 402 are connected with the anti-overflow pump P5, and two or one anti-overflow needles 402 are connected with one suction pump; when the number of anti-overflow needles 402 is large, another anti-overflow pump P5 can also be added.

[0038] In actual installation, each channel of the first suction pump P3 is connected with the first liquid collector 701, the first liquid collector 701 is connected with the waste liquid container 100 through a pipeline, so that the extracted waste liquid first enters the first liquid collector 701 and then enters the waste liquid container 100 through the first liquid collector 701; similarly, the outlet of each channel of the second suction pump P4 is connected with the second liquid collector 702, the second liquid collector 702 is connected with the waste liquid container 100 through a pipeline, so that the extracted waste liquid first enters the second liquid collector 702 and then enters the waste liquid container 100 through the second liquid collector 702, the two liquid collectors have a buffering effect and balance the pipeline pressure of the suction unit. In addition, in order to reduce the number of liquid collectors, when there are two suction units, the outlet of the second suction pump of the second suction unit can be connected to the first liquid collector of the second suction unit or the liquid collector of the other suction unit, thereby reducing the number of liquid collectors, and details are shown in Figure 4 In actual installation, the utility model discloses that the first liquid collector 701 and the third liquid collector 703 are connected through a pipeline, and a liquid level sensor 704 (of course, it can also be a weight sensor or a pressure sensor) is installed on the pipeline, when liquid is detected, it indicates that the anti-overflow needle 402 has sucked the excess liquid in the reaction cup, which further indicates that the first needle 801, the second needle 802, the first suction needle 404 and / or the second suction needle 405 are blocked.

[0039] In combination with Figure 4 It can be known that the solid-liquid separation needle 500 is connected with the first liquid collector 701 through a separation pipeline, and a separation pump P6 is arranged on the separation pipeline. In the detection process, after the magnetic beads are cleaned, a detection process is carried out by adding a substrate to carry out light detection, and after the light detection, the substrate solution often exists in the reaction cup, the solid-liquid separation needle 500 and the separation pump P6 can be used to suck the waste liquid in the reaction cup into the first liquid collector 701, and then the waste liquid after the light detection is sucked out, so that the solid-liquid separation is realized.

[0040] In actual installation, the first valve D1, the second valve D2, the third valve D3 and the fourth valve D4 of the utility model are all electromagnetic valves, the control input ends of the first valve D1, the second valve D2, the third valve D3, the fourth valve D4 and the two-position three-way electromagnetic valve 607 are connected with the control output end of the control system of the analyzer, and the opening and closing of the valves are controlled by the control system; the control input ends of the liquid injection pump P1, the suction pump, the anti-overflow pump P5, the flushing pump P2 and the separation pump P6 are connected with the control output end of the control system, so that the automatic control of the multiple pumps is realized. In actual installation, the liquid injection pump P1 is preferably a plunger pump with reciprocating suction function, of course, it can also be a syringe pump, a reciprocating pump and the like, the flushing pump P2 is preferably a diaphragm pump, of course, it can also be a peristaltic pump, a centrifugal pump and the like, and the suction pump and the anti-overflow pump P5 are preferably peristaltic pumps with multiple channels, of course, they can also be centrifugal pumps and the like.

[0041] In a more preferred embodiment of the utility model, each group of cleaning module still includes first suction and cleaning unit, first suction and cleaning unit includes first needle 801, first pump P7, fifth valve D5 which are connected through first pipeline and second needle 802, second pump P8, sixth valve D6 which are connected through second pipeline, power provided by first pump P7 and second pump P8 can extract high concentration mixed solution in reaction cup;First suction and cleaning unit still includes cleaning pool 803, first cleaning pump P9, second cleaning pump P10 and waste liquid pump P11, the import of first cleaning pump P9, the import of second cleaning pump P10 are connected with second container 300, the export of first cleaning pump P9 is divided into two ways and is connected with first pump P7, second pump P8 respectively;The export of second cleaning pump P10 is connected with the import of cleaning pool 803, the import of waste liquid pump P11 is connected with the export of cleaning pool 803 bottom, the export of waste liquid pump P11 is connected with waste liquid container 100.In actual work, the first needle 801 and the second needle 802 of the first suction and cleaning unit of the utility model are all first suction needles, two first suction needles can work alternately, and then the continuous first suction of multiple reaction cups on the reaction disc is realized, and then the pipeline detection demand of high-throughput sample is met.Wherein, in actual installation, first pump P7, second pump P8 are preferably plunger pump or reciprocating pump etc., first cleaning pump P9, second cleaning pump P10 and waste liquid pump P11 are preferably diaphragm pump, can also be centrifugal pump, peristaltic pump etc., the utility model is not limited to this.

[0042] In first suction, first needle 801 is moved to the upper of reaction cup, first pump P7 is powered on, and the mixed solution of reaction cup is extracted, first needle 801 is moved to the upper of cleaning pool 803, and first pump P7 spits the extracted mixed solution into cleaning pool 803;Similarly, the second pump and the second needle can be used for suction;First cleaning pump P9, second cleaning pump P10, waste liquid pump P11 and fifth valve D5, sixth valve D6 are powered on, and first needle 801 and second needle 802 are cleaned respectively, and the waste liquid generated in cleaning is pumped into waste liquid container 100 by waste liquid pump P11, and the internal cleaning and external cleaning are carried out, in the internal cleaning process, to ensure the cleaning effect of first pump P7, first cleaning pump P9 can be closed, purified water is pumped by first pump P7, and then the purified water is spit out, so that the cleaning of first pump P7 and first needle 801 is realized repeatedly and the cleaning effect is ensured, and the cleaned first needle 801 can be used for first suction again.

[0043] In this process, the pressure detection module preferably pressure sensor monitors first suction pressure in first suction process, if first suction pressure exceeds upper preset threshold value, then it shows that pipeline is blocked;If first suction pressure is less than lower preset threshold value, it shows that mixed solution in reaction cup is not extracted as much as possible, and it shows that there is certain residue in reaction cup.

[0044] In actual installation, the fifth valve D5 and the sixth valve D6 are both electromagnetic valves, the control input ends of the first pump P7, the second pump P8, the first cleaning pump P9, the second cleaning pump P10 and the waste liquid pump P11 are connected with the control output end of the control system, and the start and stop of each pump are controlled by the control system.

[0045] The utility model has two cleaning modules, the liquid injection pump P1 of two cleaning modules is connected in parallel at one port of two position three way valve, another port of two position three way electromagnetic valve 607 is connected with first container 200, its third port is connected with second container 300, can realize two liquid injection pump P1's liquid and maintenance of going up simultaneously. Since the magnetic bead cleaning fluid is crystalline solution, usually needs to flush and maintain pipeline and liquid injection needle after filling magnetic bead cleaning fluid, avoids the pipeline and liquid injection needle crystallization and appears to block, specific maintenance is as follows:

[0046] Firstly, the two-position three-way electromagnetic valve 607 is switched to the state of being communicated with the second container 300, the flushing pump P2 is powered on to provide power, the two first valves D1, the two second valves D2, the two second exhaust valves D8 and the two first exhaust valves D7 are powered on, the purified water is pumped into the first liquid distributor 601 and the second liquid distributor 602, and enters the waste liquid container 100 through the first exhaust pipeline 605 and the second exhaust pipeline 606 respectively, and the bubbles in the two liquid distributors are exhausted.

[0047] Secondly, the flushing pump P2 is powered off (after power off, the branch is in a state that can flow under external force), the two liquid injection pumps P1 are powered on at the same time, the second valve D2 is powered off and the exhaust valve is powered off, the liquid injection pump P1 is filled with water; the second valve D2 is powered on and the first valve D1 is powered off, the third valve D3 and the fourth valve D4 are powered on, the liquid injection pump P1 injects purified water into each first liquid injection needle 401 and second liquid injection needle 403, the purified water is injected into the reaction disc placed in advance through the liquid injection needle, the internal cleaning of the first liquid injection needle and the second liquid injection needle is realized, and the liquid in the reaction disc is pumped to the waste liquid container 100 by using the anti-overflow needle 402, the first suction needle 404 and the second suction needle 405, so that the cleaning and maintenance of each liquid injection needle, suction needle and anti-overflow needle can be realized for a certain period of time; of course, in the maintenance process, the liquid injection needle and the suction needle, the anti-overflow needle 402 can also be soaked in the purified water in the reaction disc, so as to avoid crystallization as much as possible.

[0048] The reaction cup seat (reaction cup card) of the analyzer is generally rotatable, the suction and injection needle group 400 can be moved to the lower side of the reaction disc, and the continuous suction and injection cleaning of multiple reaction cups is realized by rotating the reaction cup, and the specific process is as follows: after maintenance, the two three-way electromagnetic valves 607 are switched to the state of connecting the liquid injection pump P1 and the first container 200, the liquid injection pump P1 and the first valve D1 are powered on, the flushing pump P2 is powered off, the second valve D2, the third valve D3 and the fourth valve D4 are all powered off, and the plunger pump extracts the magnetic bead cleaning liquid in the first container 200; then the first valve D1 is powered off, the second valve D2, the third valve D3 and the fourth valve D4 are powered on, so that the magnetic bead cleaning liquid is injected into each injection needle through the liquid injection pipeline after the liquid distributor, and the simultaneous injection of multiple injection needles is realized; if there is no reaction cup below the injection needle, the valve on the injection pipeline corresponding to the injection needle is in a powered-off state; after injection, the reaction disc is rotated by a certain angle to make the reaction cup correspondingly located below the suction needle, the suction pump and the anti-overflow pump P5 are powered on, the waste liquid after cleaning is sucked into the waste liquid container 100, and suction is realized; the reaction cup after cleaning enters the light measurement module of the analyzer, the substrate is added, light measurement is performed, the reaction cup returns to the reaction disc again after light measurement, and the waste liquid is sucked out by using the solid-liquid separation needle 500.

[0049] Finally, it should be emphasized that the above only describes the preferred embodiments of the present application, and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments without creative labor, or equivalently replace some technical features. Thus, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A magnetic bead washing system for a chemiluminescent immunoassay analyzer, comprising a waste liquid container, a first container containing a magnetic bead washing solution, and a second container containing a pipeline washing solution, characterized in that: Further comprising at least one set of washing modules for washing magnetic beads in the reaction cup, the washing module comprising a plurality of sets of suction and injection needle groups, solid-liquid separation needles, liquid injection and washing units, and liquid suction units, each set of the suction and injection needle groups comprising a first liquid injection needle, an anti-overflow needle, a second liquid injection needle, a first suction needle, and a second suction needle arranged in sequence; The liquid injection and washing unit comprises a liquid injection pump, the inlet side of the liquid injection pump is provided with a first valve, the inlet of the first valve has two branches, one of which is connected with the first container, the other is connected with the second container through an internal washing pipeline, and the internal washing pipeline is provided with a flushing pump; the outlet side of the liquid injection pump is provided with a second valve, the outlet of the second valve is connected with a first distributor and a second distributor in parallel, the first distributor is connected with the first liquid injection needle through a first liquid injection pipeline, each first liquid injection pipeline is provided with a third valve, the second distributor is connected with the second liquid injection needle through a second liquid injection pipeline, each second liquid injection pipeline is provided with a fourth valve; The first suction needle, the second suction needle, and the anti-overflow needle are connected with the liquid suction unit.

2. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 1, characterized by: The liquid suction unit comprises a first suction pump, a second suction pump, and an anti-overflow pump, the outlet ends of which are connected with the waste liquid container, the first suction needle is connected with the suction channel of the first suction pump through a first suction pipeline, each second suction needle is connected with the suction channel of the second suction pump through a second suction pipeline; A plurality of anti-overflow needles are connected with the anti-overflow pump through an anti-overflow pipeline, or at least one anti-overflow needle is connected with the first suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump, or at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump, or at least one anti-overflow needle is connected with the first suction pump, at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump.

3. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 2, characterized by: At least one anti-overflow needle is connected with the first suction pump, at least one anti-overflow needle is connected with the second suction pump, and the remaining anti-overflow needles are connected with the anti-overflow pump.

4. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 2, characterized by: The first suction pump is connected with the waste liquid container through a first liquid collector, the second suction pump is connected with the waste liquid container through a second liquid collector, the solid-liquid separation needle is connected with the first liquid collector or the second liquid collector through a separation pipeline, and the separation pipeline is provided with a separation pump.

5. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 4, characterized by: The outlet of the anti-overflow pump is connected with the waste liquid container through a third liquid collector. A liquid level sensor or a weight sensor is arranged between the third liquid collector and the first liquid collector or between the third liquid collector and the second liquid collector.

6. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 1, characterized by: The two branches of the inlet side of the first valve are connected through a two-position three-way electromagnetic valve.

7. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 1, characterized by: The first distributor is connected with the waste liquid container through a first exhaust pipeline, the second distributor is connected with the waste liquid container through a second exhaust pipeline, the first exhaust pipeline is provided with a first exhaust valve, and the second exhaust pipeline is provided with a second exhaust valve.

8. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 1, characterized by: Each of the cleaning modules further comprises a first suction and cleaning unit, which comprises a first needle, a first pump, a fifth valve connected by a first pipeline, and a second needle, a second pump, a sixth valve connected by a second pipeline; the first suction and cleaning unit further comprises a cleaning tank, a first cleaning pump, a second cleaning pump, and a waste liquid pump, the inlet of the first cleaning pump and the inlet of the second cleaning pump are connected with the second container, the outlet of the first cleaning pump is divided into two paths and is connected with the first pump and the second pump respectively; the outlet of the second cleaning pump is connected with the inlet of the cleaning tank, the inlet of the waste liquid pump is connected with the outlet at the bottom of the cleaning tank, and the outlet of the waste liquid pump is connected with the waste liquid container.

9. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 8, characterized by: Pressure detection modules are arranged between the first needle and the first pump and between the second needle and the second pump.

10. The magnetic bead washing system for a chemiluminescence immunoassay analyzer according to claim 1, characterized by: The bottom opening height of the first liquid injection needle and the second liquid injection needle is consistent, the bottom opening height of the first suction needle is higher than that of the second suction needle, and the bottom opening of the anti-overflow needle is lower than that of the first liquid injection needle and higher than that of the first suction needle.

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