Exosome extraction equipment

Abstract

The utility model provides exosome extraction equipment, and relates to the field of exosome extraction, the exosome extraction equipment comprises an extraction unit, the bottom of the extraction unit is provided with a power unit, the extraction unit comprises an extraction tank, and the top of the extraction tank is movably connected with a tank cover; according to the exosome extraction device, the filter screen can be supported through the connecting cylinder and can intercept impurities in body fluid, the intercepted body fluid can enter the connecting disc, and exosome in the body fluid is intercepted through the ultrafiltration membrane cloth, so that the exosome extraction operation is realized; in the extraction process, an output shaft of a servo motor drives a winding wheel to frequently rotate in the forward and reverse directions, the winding wheel drives a connecting rope to be wound and unwound, the connecting rope drives one side of a movable disc to move up and down, the movable disc compresses a spring, and the movable disc can drive the connecting disc and the ultrafiltration membrane cloth to frequently shake through cooperation of the spring and the connecting rope; therefore, exosomes on the ultrafiltration membrane cloth can move, and the condition that the extraction efficiency is reduced due to blockage is effectively prevented.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the exosome extraction field, concretely is a kind of exosome extraction equipment. BACKGROUND

[0002] Exosome extraction equipment is used to extract exosomes from biological samples, exosomes are small vesicles secreted by cells, naturally exist in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk, can provide important information about cell health and disease state, and play an important role in intercellular communication and disease marker research.

[0003] Among them, when exosomes are extracted, first, the body fluid is injected into the extraction equipment, and the body fluid is filtered by the filter cloth inside the extraction equipment, since the exosomes are small, a filter cloth with small filter holes is used, so that the liquid moisture passes through the filter cloth, and the exosomes are left on the filter cloth, then the filter cloth is taken out to collect the exosomes on the filter cloth, to realize the extraction of exosomes. But in actual use, since the volume of exosomes is small, the filter cloth used is small, and the filter cloth is easily clogged during filtration and extraction, thereby reducing the extraction efficiency of exosomes.

[0004] Therefore, the utility model provides an exosome extraction equipment to solve the above problems. INVENTION CONTENTS

[0005] To solve the above technical problems, the utility model provides the following technical scheme:

[0006] An exosome extraction equipment, comprising an extraction unit, a power unit is arranged at the bottom of the extraction unit, the extraction unit comprises an extraction tank, a tank cover is movably connected to the top of the extraction tank, a connecting barrel is fixedly connected to the top of the inner cavity of the tank cover, a filter screen is threadedly connected in the inner cavity of the connecting barrel, an active disc is movably connected to the upper end of the inner cavity of the extraction tank, a connecting disc is arranged at the top of the active disc, an ultrafiltration membrane cloth is fixedly connected to the lower end of the inner cavity of the connecting disc, the power unit comprises a base, a servo motor is fixedly connected to the bottom of the inner cavity of the base, a winding wheel is drivingly connected to the output shaft of the servo motor, a connecting rope is arranged on the surface of the winding wheel, a fixed disc is fixedly connected to the lower end of the inner cavity of the extraction tank, and a spring is fixedly connected to the top of the fixed disc.

[0007] Further, in the utility model, the top of the base is fixedly connected with the bottom of the extraction tank, one end of the connecting rope away from the winding wheel is fixedly connected with one side of the bottom of the active disc, and the top of the spring is fixedly connected with the bottom of the active disc.

[0008] Further, in the utility model, the top of the tank cover is connected with a liquid inlet pipe, the lower end of the extraction tank surface is connected with a liquid outlet pipe, the surfaces of the liquid inlet pipe and the liquid outlet pipe are movably connected with round covers, and the top of the connecting disc and the bottom of the filter screen are fixedly connected with round rods.

[0009] Further, in the utility model, the bottom of the connecting disc and the top of the movable disc are fixedly connected with magnet sheets, the two magnet sheets are in contact on the side close to each other, and are magnetically connected.

[0010] Further, in the utility model, the top of the fixed disc is provided with a through hole, the bottom of the extraction tank inner cavity is fixedly connected with a sealing ring, the connecting rope penetrates the through hole and the inner cavity of the sealing ring and is movably connected with the through hole and the inner cavity of the sealing ring, and the sealing ring is in contact with the connecting rope.

[0011] Further, in the utility model, one side of the base inner cavity is provided with a limiting wheel, one side of the limiting wheel is movably connected with a supporting plate through a bearing, the bottom of the supporting plate is fixedly connected with the bottom of the base inner cavity, the connecting rope is located in the inner cavity of the limiting wheel and is movably connected with the inner cavity of the limiting wheel.

[0012] Beneficial effects, the utility model has the following beneficial effects:

[0013] The utility model discloses a filter screen support can be connected to the cylinder, and the filter screen can intercept the impurities in the body fluid, and the intercepted body fluid can enter the inside of the connecting disc, and the exosome in the body fluid is intercepted through the ultrafiltration membrane cloth, so that the extraction operation of the exosome is realized, the winding wheel is driven to rotate frequently in positive and negative directions through the output shaft of the servo motor in the extraction process, the winding wheel drives the connecting rope to be retracted and released, the connecting rope drives one side of the movable disc to move up and down, the movable disc compresses the spring, the movable disc drives the connecting disc and the ultrafiltration membrane cloth to shake frequently through the cooperation of the spring and the connecting rope, and the exosome on the ultrafiltration membrane cloth can move, so that the situation that the extraction efficiency is reduced due to blockage is effectively prevented. ACCOUT OF DRAWINGS

[0014] Figure 1 It is the structure schematic diagram of the utility model;

[0015] Figure 2 It is the extraction tank cross section state structure schematic diagram of the utility model;

[0016] Figure 3 It is the base cross section state structure schematic diagram of the utility model;

[0017] Figure 4 It is the movable disc, connecting disc, spring and fixed disc connecting structure schematic diagram of the utility model;

[0018] Figure 5It is the utility model tank cover elevation state structure schematic diagram.

[0019] In the figure,

[0020] 1, extraction unit;11, extraction tank;111, drain pipe;12, tank cover;121, liquid inlet pipe;13, connecting cylinder;14, filter screen;15, movable disc;16, connecting disc;161, magnet sheet;17, ultrafiltration membrane cloth;2, power unit;21, base;22, servo motor;23, winding wheel;24, connecting rope;241, limit wheel;25, fixed disc;26, spring. DETAILED DESCRIPTION

[0021] In order to more understand the technical content of the utility model, specific embodiment is raised and the following is described with the attached drawings.The aspects of the utility model are described in the disclosure with reference to the attached drawings, and many embodiments of the description are shown in the drawings.The embodiments of the disclosure are not necessarily defined in all aspects including the utility model.It should be understood that the various concepts and embodiments introduced above, and those concepts and embodiments described in more detail below can be implemented in any one of many ways, because the concepts and embodiments disclosed by the utility model are not limited to any implementation manner.In addition, some aspects of the utility model can be used alone, or used in any appropriate combination with other aspects of the utility model.

[0022] Example 1

[0023] As Figures 1-5 shown, it is the first embodiment of the utility model, and the embodiment provides an exosome extraction device, which comprises an extraction unit 1, the bottom of the extraction unit 1 is provided with a power unit 2, the extraction unit 1 comprises an extraction tank 11, and the top of the extraction tank 11 is movably connected with a tank cover 12, the top of the inner cavity of the tank cover 12 is fixedly connected with a connecting cylinder 13, and the inner cavity of the connecting cylinder 13 is threadedly connected with a filter screen 14, the upper end of the inner cavity of the extraction tank 11 is movably connected with a movable disc 15, and the top of the movable disc 15 is provided with a connecting disc 16, and the lower end of the inner cavity of the connecting disc 16 is fixedly connected with an ultrafiltration membrane cloth 17, the power unit 2 comprises a base 21, and the bottom of the inner cavity of the base 21 is fixedly connected with a servo motor 22, the output shaft of the servo motor 22 is drivingly connected with a winding wheel 23, and the surface of the winding wheel 23 is provided with a connecting rope 24, the lower end of the inner cavity of the extraction tank 11 is fixedly connected with a fixed disc 25, and the top of the fixed disc 25 is fixedly connected with a spring 26.

[0024] As Figures 1-5As shown, the filter screen 14 can be supported by the connecting cylinder 13, and the filter screen 14 can intercept impurities in the body fluid, and the intercepted body fluid can enter the inside of the connecting disc 16, and the exosomes in the body fluid can be intercepted by the ultrafiltration membrane cloth 17, so as to realize the extraction operation of the exosomes. During the extraction process, the servo motor 22 drives the winding wheel 23 to frequently rotate forward and reverse, so that the winding wheel 23 drives the connecting rope 24 to be wound and unwound, the connecting rope 24 drives one side of the movable disc 15 to move up and down, the movable disc 15 compresses the spring 26, and the cooperation of the spring 26 and the connecting rope 24 can make the movable disc 15 drive the connecting disc 16 and the ultrafiltration membrane cloth 17 to frequently shake, so that the exosomes on the ultrafiltration membrane cloth 17 move, and the situation that the extraction efficiency is reduced due to blockage is effectively prevented.

[0025] Embodiment 2

[0026] Referring to Figure 1 , 2 , 4 and 5, it is a second embodiment of the utility model, and the embodiment is based on the previous embodiment.

[0027] In the embodiment, the top of the base 21 is fixedly connected with the bottom of the extraction tank 11, one end of the connecting rope 24 away from the winding wheel 23 is fixedly connected with one side of the bottom of the movable disc 15, and the top of the spring 26 is fixedly connected with the bottom of the movable disc 15.

[0028] The top of the tank cover 12 is communicated with a liquid inlet pipe 121, and the lower end of the surface of the extraction tank 11 is communicated with a liquid outlet pipe 111. The surfaces of the liquid inlet pipe 121 and the liquid outlet pipe 111 are movably connected with round covers, and the top of the connecting disc 16 and the bottom of the filter screen 14 are fixedly connected with round rods.

[0029] The bottom of the connecting disc 16 and the top of the movable disc 15 are fixedly connected with magnet pieces 161, and the sides of the two magnet pieces 161 close to each other are in contact and magnetically connected.

[0030] As shown in Figure 1 , 2 , 4 and 5, the connecting disc 16 and the filter screen 14 can be driven to move by the round rods, the body fluid can be transmitted by the liquid inlet pipe 121, the liquid water after the exosomes are extracted can be discharged by the liquid outlet pipe 111, the upper magnet piece 161 is driven downward by the connecting disc 16, the upper magnet piece 161 is in contact with the lower magnet piece 161, and then the two magnet pieces are magnetically connected, so that the connection firmness of the connecting disc 16 and the movable disc 15 is effectively ensured.

[0031] Embodiment 3

[0032] Referring to Figure 3 , it is a third embodiment of the utility model, and the embodiment is based on the previous two embodiments.

[0033] In this embodiment, the top of the fixed disc 25 is provided with a through hole, and the bottom of the inner cavity of the extraction tank 11 is fixedly connected with a sealing ring. The connecting rope 24 penetrates the through hole and the inner cavity of the sealing ring, and is movably connected with the through hole and the inner cavity of the sealing ring. The sealing ring is in contact with the connecting rope 24.

[0034] One side of the inner cavity of the base 21 is provided with a limiting wheel 241, and one side of the limiting wheel 241 is movably connected with a supporting plate through a bearing. The bottom of the supporting plate is fixedly connected with the bottom of the inner cavity of the base 21. The connecting rope 24 is located in the inner cavity of the limiting wheel 241 and is movably connected with the inner cavity of the limiting wheel 241.

[0035] As shown in Figure 3 , the limiting wheel 241 can be limited and supported by the supporting plate. When the connecting rope 24 moves, it will slide along the inner cavity of the limiting wheel 241. Thus, the limiting wheel 241 can limit the connecting rope 24. During the movement of the connecting rope 24, it is in contact with the sealing ring. Thus, the liquid in the extraction tank 11 will not enter the inside of the base 21 through the gap.

[0036] In use, first, the body fluid can be transmitted to the inner cavity of the connecting cylinder 13 through the liquid inlet pipe 121. The impurities in the body fluid can be intercepted by the filter screen 14 in the connecting cylinder 13. The intercepted body fluid will enter the inside of the connecting disc 16 downward. The exosomes in the body fluid are intercepted by the ultrafiltration membrane cloth 17, so that the exosomes are on the ultrafiltration membrane cloth 17. In this way, the extraction of exosomes is realized. During the extraction process, the output shaft of the servo motor 22 drives the winding wheel 23 to rotate frequently in forward and reverse directions, so that the winding wheel 23 drives the connecting rope 24 to be wound and unwound. The connecting rope 24 drives one side of the movable disc 15 to move up and down, so that the movable disc 15 compresses the spring 26. The cooperation of the spring 26 and the connecting rope 24 can make the movable disc 15 drive the connecting disc 16 and the ultrafiltration membrane cloth 17 to shake frequently, so that the exosomes on the ultrafiltration membrane cloth 17 move. This can effectively prevent the situation that the extraction efficiency is reduced due to blockage. After the extraction is completed, the tank cover 12 is pulled to separate the tank cover 12 from the extraction tank 11, so that the extraction tank 11 is opened. Then, the round rod on the top of the connecting disc 16 is taken out. The round rod drives the connecting disc 16 and the movable disc 15 to separate, so that the connecting disc 16 drives the ultrafiltration membrane cloth 17 to move to the outside of the extraction tank 11. This not only facilitates the collection of the exosomes on the ultrafiltration membrane cloth 17, but also facilitates the cleaning operation of the ultrafiltration membrane cloth 17.

[0037] The standard parts used in the application file can be purchased from the market, and can be ordered according to the description and drawings, and the specific connection mode of each part adopts the conventional means such as bolt, rivet and welding in the prior art, the mechanical, part and equipment adopt the conventional type in the prior art, the control mode is automatically controlled through the controller, the control circuit of the controller can be realized through simple programming of the person skilled in the art, which belongs to the common knowledge in the art, and the application is mainly used to protect the mechanical device, so the control mode and circuit connection are not explained in detail.

[0038] Although the utility model has disclosed as above with preferable embodiment, it is not used to limit the utility model. Those who have ordinary knowledge in the technical field to which the utility model belongs can make various changes and decorations without departing from the spirit and scope of the utility model. Therefore, the protection scope of the utility model shall be subject to the definition of the claims.

Claims

1. An exosome extraction device comprising an extraction unit (1), characterized in that: The bottom of the extraction unit (1) is provided with a power unit (2), the extraction unit (1) comprises an extraction tank (11), and the top of the extraction tank (11) is movably connected with a tank cover (12); the inner cavity of the top of the tank cover (12) is fixedly connected with a connecting barrel (13), and the inner cavity of the connecting barrel (13) is threadedly connected with a filter screen (14); the upper end of the inner cavity of the extraction tank (11) is movably connected with a movable disc (15), and the top of the movable disc (15) is provided with a connecting disc (16); the inner cavity of the lower end of the connecting disc (16) is fixedly connected with an ultrafiltration membrane cloth (17); the power unit (2) comprises a base (21), and the bottom of the inner cavity of the base (21) is fixedly connected with a servo motor (22); the output shaft of the servo motor (22) is transmissionally connected with a winding wheel (23), and the surface of the winding wheel (23) is provided with a connecting rope (24); the lower end of the inner cavity of the extraction tank (11) is fixedly connected with a fixed disc (25), and the top of the fixed disc (25) is fixedly connected with a spring (26).

2. The exosome extraction device of claim 1, wherein: The top of the base (21) is fixedly connected with the bottom of the extraction tank (11), one end of the connecting rope (24) away from the winding wheel (23) is fixedly connected with one side of the bottom of the movable disc (15), and the top of the spring (26) is fixedly connected with the bottom of the movable disc (15).

3. The exosome extraction device of claim 1, wherein: The top of the tank cover (12) is communicated with a liquid inlet pipe (121), and the lower end of the surface of the extraction tank (11) is communicated with a liquid outlet pipe (111); the surfaces of the liquid inlet pipe (121) and the liquid outlet pipe (111) are movably connected with round covers; the top of the connecting disc (16) and the bottom of the filter screen (14) are fixedly connected with round rods.

4. The exosome extraction device of claim 1, wherein: The bottom of the connecting disc (16) and the top of the movable disc (15) are fixedly connected with magnet pieces (161), and the two magnet pieces (161) are in contact on one side close to each other and are magnetically connected.

5. The exosome extraction device of claim 1, wherein: The top of the fixed disc (25) is provided with a through hole, and the bottom of the inner cavity of the extraction tank (11) is fixedly connected with a sealing ring; the connecting rope (24) penetrates through the through hole and the inner cavity of the sealing ring and is movably connected with the through hole and the inner cavity of the sealing ring, and the sealing ring is in contact with the connecting rope (24).

6. The exosome extraction device of claim 1, wherein: One side of the inner cavity of the base (21) is provided with a limiting wheel (241), one side of the limiting wheel (241) is movably connected with a supporting plate through a bearing, and the bottom of the supporting plate is fixedly connected with the bottom of the inner cavity of the base (21); the connecting rope (24) is located in the inner cavity of the limiting wheel (241) and is movably connected with the inner cavity of the limiting wheel (241).

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