Rapid detection kit for microorganisms

Abstract

The utility model relates to a rapid microorganism detection kit, which comprises an upper shell, a lower shell and a test strip, the lower shell is internally provided with a clamping groove for accommodating the test strip, the test strip is arranged in the lower shell, the upper shell is provided with a sample adding hole and a result display area, the result display area is provided with a baffle plate, and the baffle plate is arranged on the lower shell. And a colorimetric area is arranged on one side of the upper surface of the baffle plate. According to the rapid microorganism detection kit disclosed by the utility model, the baffle is arranged in the result display area, when detection is carried out, a sample to be measured is dropwise added into the sample adding hole, and after reaction is completed, the baffle is opened, or the sample directly passes through the transparent baffle; the display color observed by the window of the result display area is compared with the colorimetric area arranged on the upper surface of the baffle plate, and the detection result is read, so that the possibility that the interior of the shell and the test paper are polluted in the detection process is reduced, the detection accuracy is improved, and the operation is simple and convenient.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection, and in particular to a rapid microbial detection kit. Background Technology

[0002] Microbial detection refers to the process of identifying, classifying, quantifying, or analyzing the characteristics of microorganisms (such as bacteria, viruses, fungi, parasites, etc.) using scientific methods. These microorganisms may exist in various samples such as human bodies, food, water, soil, air, and industrial products. Microbial detection kits are widely used in fields such as medical health, food safety, environmental monitoring, and industrial production.

[0003] For example, Chinese Patent Publication No. CN221804025U discloses a test kit, comprising: a test strip, which includes a sampling section and a detection section; and a shell, which includes an interlocking plate-shaped upper shell and a lower shell, with the test strip located between the upper shell and the lower shell; wherein: the upper shell corresponding to the sampling section has an injection hole and a liquid injection hole, with the liquid injection hole located in front of the injection hole; the lower shell has a carrier plate integrally connected to the lower shell and detachable from the lower shell by pressure at a position corresponding to the liquid injection hole, and a drying tray is fixed on the upper surface of the carrier plate; when the carrier plate is integral with the lower shell, the drying tray is separated from the sampling section. By setting a destructibly detachable carrier plate in the lower shell and a drying tray inside the carrier plate, after the sample is tested, the drying tray comes into contact with the test strip by breaking the carrier plate, thereby strongly adsorbing liquid and keeping the test kit dry after testing. Therefore, the existing technology has at least the following drawbacks: on the one hand, the inside of the casing is connected to the external environment through the observation window, which may lead to contamination of the inside of the casing and the test strip during the detection process, affecting the detection results; on the other hand, it is not convenient to compare with the colorimetric card after the detection is completed. Utility Model Content

[0004] Therefore, this invention provides a rapid microbial detection kit to overcome the problems of contamination during the detection process in the prior art, which may affect the detection results and is not convenient enough.

[0005] To achieve the above objectives, this utility model provides a rapid microbial detection kit, comprising an upper shell, a lower shell, and a test strip. The lower shell has a slot for accommodating the test strip, and the test strip is disposed within the lower shell. The upper shell has a sample application hole and a result display area. The result display area has a baffle, and a colorimetric area is provided on one side of the upper surface of the baffle.

[0006] Furthermore, the bottom surface of the result display area is lower than the upper surface of the upper housing to form a recessed area. The result display area is provided with a first window and a second window. The length of the baffle is less than the length of the recessed area and greater than the distance between the two distal ends of the first window and the second window. The baffle is used to be embedded in the recessed area to slide within the recessed area.

[0007] Furthermore, the sample loading port includes an upper port and a lower port, the opening area of ​​the upper port is larger than the opening area of ​​the lower port, and the upper port and the lower port are connected by an arc surface.

[0008] Furthermore, the slot includes a support structure and a limiting structure, both of which are fixedly connected to the lower housing. The support structure includes several support bars, and the limiting structure includes a limiting block and a limiting baffle.

[0009] Furthermore, the lower surface of the upper housing is provided with a plurality of connecting posts, and the lower housing is provided with a plurality of connectors corresponding to the connecting posts. The connecting posts can be inserted into the connectors, and the connecting posts are interference-fitted with the plugs.

[0010] Furthermore, the test strip includes a sample area, a reaction display area, and an absorbent area arranged sequentially along the chromatographic direction of the sample to be tested. The sample area includes a sample pad and a conjugation pad arranged sequentially along the chromatographic direction of the sample to be tested. The reaction display area is provided with a nitrocellulose membrane. The nitrocellulose membrane is provided with a test line and a control line along the chromatographic direction of the sample to be tested. The absorbent area is provided with an absorbent pad.

[0011] Furthermore, the conjugate pad is provided with colorimetric microspheres through physical adsorption, and the number of test lines is at least one.

[0012] Furthermore, the baffle is made of colorless transparent glass or colorless transparent plastic.

[0013] Furthermore, the sample application well is located directly above the sample pad, and the result display area is located directly above the reaction display area.

[0014] Furthermore, both the upper housing and the lower housing are made of plastic.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] This novel rapid microbial detection kit features a baffle in the result display area. During testing, the sample to be measured is added to the sample well, and the baffle is opened after the reaction is complete. Alternatively, the result can be read directly through the transparent baffle by comparing the color observed in the result display window with the colorimetric area set on the upper surface of the baffle. This avoids the possibility of contamination inside the casing and the test strip during the testing process, improving detection accuracy while being simple and convenient to operate. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the rapid microbial detection kit of this utility model;

[0018] Figure 2 This is an exploded view of the rapid microbial detection kit of this utility model;

[0019] Figure 3 This is a schematic diagram of the upper shell of this utility model;

[0020] Figure 4 This is a schematic diagram of the bottom surface of the upper shell of this utility model;

[0021] Figure 5 This is a schematic diagram of the structure of the lower shell of this utility model;

[0022] Figure 6 This is a schematic diagram of the structure of the test strip of this utility model;

[0023] Figure 7 This is another embodiment of the upper shell of this utility model;

[0024] In the diagram: 1. Upper shell; 2. Lower shell; 3. Test strip; 4. Sample application port; 5. Result display area; 6. Baffle; 7. Colorimetric area; 8. Bottom surface of the result display area; 9. Upper surface of the upper shell; 10. First window; 11. Second window; 12. Upper port; 13. Lower port; 14. Curved surface; 15. Support strip; 16. Limiting block; 17. Limiting baffle; 18. Connecting column; 19. Connector; 20. Sample area; 21. Reaction display area; 22. Absorbent area; 23. Sample pad; 24. Binding pad; 25. Nitrocellulose membrane; 26. Test line; 27. Control line; 28. Absorbent pad. Detailed Implementation

[0025] To make the objectives and advantages of this utility model clearer, the utility model will be further described below with reference to the embodiments; it should be understood that the specific embodiments described herein are only for explaining this utility model and are not intended to limit this utility model.

[0026] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0027] It should be noted that in the description of this utility model, the terms "upper", "lower", "left", "right", "inner", "outer", etc., indicating the direction or positional relationship are based on the direction or positional relationship shown in the drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this utility model.

[0028] Furthermore, it should be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0029] Please see Figures 1-6 As shown, this utility model provides a rapid microbial detection kit, including an upper shell 1, a lower shell 2 and a test strip 3. The lower shell 2 is provided with a slot for accommodating the test strip 3, and the test strip 3 is disposed in the lower shell 2. The upper shell 1 is provided with a sample application hole 4 and a result display area 5. The result display area 5 is provided with a baffle 6, and a colorimetric area 7 is provided on one side of the upper surface 9 of the baffle 6.

[0030] This novel rapid microbial detection kit features a baffle 6 in the result display area 5. During testing, the sample to be measured is added to the sample application well 4. After the reaction is complete, the baffle 6 is opened, or the result is read directly through the transparent baffle 6. The color observed through the window of the result display area 5 is compared with the colorimetric area 7 set on the upper surface of the baffle 6. This avoids the possibility of contamination inside the casing and the test strip during the testing process, improving the accuracy of the test while making the operation simple and convenient.

[0031] Please see Figure 2 and Figure 3 The bottom surface 8 of the result display area 5 is lower than the upper surface 9 of the upper housing 1 to form a recessed area. The result display area 5 is provided with a first window 10 and a second window 11. The length of the baffle 6 is less than the length of the recessed area and greater than the distance between the two distal ends of the first window 10 and the second window 11. The baffle 6 is used to be embedded in the recessed area to slide within the recessed area.

[0032] This invention features a baffle 6 in the display area window of the reagent kit. Through physical isolation, it effectively prevents external contaminants (such as dust, droplets, or accidental touches during operation) from interfering with the detection area, ensuring the cleanliness of the reaction system. At the same time, it can reduce the interference of ambient light, humidity, or temperature fluctuations on the color development results and improve the accuracy of interpretation.

[0033] As one implementation method, such as Figure 3 As shown, there are two display windows, namely the first window 10 and the second window 11.

[0034] Specifically, both the first window 10 and the second window 11 are long rectangular strips, corresponding to the shape of the test strip 3.

[0035] It is understood that the length of the baffle 6 of this invention is less than the length of the recessed area and greater than the distance between the two far ends of the first window 10 and the second window 11. This allows the baffle 6 of this invention to cover the first window 10 and the second window 11 during testing. At the same time, when comparison is needed after testing, the baffle 6 can be slid so that the color comparison area is aligned with the first window 10 or the second window 11 for better color comparison.

[0036] As another implementation method, please refer to Figure 7 There is only one display window, namely the first window 10.

[0037] In another implementation, there may be three display windows, namely the first window 10, the second window 11, and the third window.

[0038] In other words, this utility model does not specifically limit the number of observation windows. Any number of observation windows that allow the color of the test strip 3 to be observed through the display window are within the protection scope of this utility model.

[0039] Specifically, the sample loading port 4 includes an upper port 12 and a lower port 13. The area of ​​the opening of the upper port 12 is larger than the area of ​​the opening of the lower port 13. The upper port 12 and the lower port 13 are connected by an arc surface 14.

[0040] The sample application well 4 of this reagent kit features an arc-shaped connection 14 between its upper port 12 and lower internal port 13. This design not only guides sample droplets into the well precisely through a smooth transition, reducing the risk of sample splashing, residue buildup, or air bubbles during application, but also prevents cross-contamination caused by liquid residue. The arc-shaped 14 structure also optimizes fluid dynamics, allowing for more uniform wetting of the test strip 3 with trace amounts of sample, thus improving detection sensitivity and repeatability. Furthermore, this design is compatible with different application tools (such as droppers and pipettes), reducing operational difficulty and making it particularly suitable for non-professionals to quickly learn, ensuring an efficient and reliable testing process.

[0041] Please see Figure 5 The slot includes a support structure and a limiting structure. Both the support structure and the limiting structure are fixedly connected to the lower housing 2. The support structure includes several support bars 15, and the limiting structure includes a limiting block 16 and a limiting baffle 17.

[0042] Understandably, the limiting block 16 and limiting baffle 17 within the kit provide multiple limiting functions for the test strip 3 through precise physical constraint design: First, they fix the position of the test strip 3, preventing it from shifting laterally or tilting during operation or liquid immersion, ensuring that the reaction display area is strictly aligned with the sample flow path, and improving the consistency of test results; Second, the limiting structure buffers the interference of external vibration or movement on the test strip 3, reducing the risk of false negatives or false positives caused by displacement; Third, the limiting baffle 17 prevents the test strip 3 from directly contacting the inner wall of the kit, avoiding frictional damage or accidental contamination.

[0043] Please see Figure 4 and Figure 5 The lower surface of the upper housing 1 is provided with a plurality of connecting posts 18, and the lower housing 2 is provided with a plurality of connectors 19 corresponding to the connecting posts 18. The connecting posts 18 can be inserted into the connectors 19, and the connecting posts 18 are interference-fitted with the plug.

[0044] In one embodiment, see Figure 4 and Figure 5 There are 6 connecting posts 18 and 6 connectors 19.

[0045] In another embodiment, the number of connecting posts 18 and connectors 19 may also be 4 or 8.

[0046] In other words, this utility model does not impose a specific limit on the number of connecting posts 18 and connectors 19. Those skilled in the art can selectively set them according to the size and shape of the reagent kit. Any number of connecting posts 18 and connectors 19 that can achieve the connection and fixation between the upper shell 1 and the lower shell 2 through connecting posts 18 and connectors 19 are within the protection scope of this utility model.

[0047] Please see Figure 6 The test strip 3 includes a sample area 20, a reaction display area 21, and an absorbent area 22 arranged sequentially along the chromatographic direction of the sample to be tested. The sample area 20 includes a sample pad 23 and a conjugate pad 24 arranged sequentially along the chromatographic direction of the sample to be tested. The reaction display area 21 is provided with a nitrocellulose membrane 25. The nitrocellulose membrane 25 is provided with a test line 26 and a control line 27 along the chromatographic direction of the sample to be tested. The absorbent area 22 is provided with an absorbent pad 28.

[0048] Please see Figure 6 Arrow A points in the direction of the chromatography of the sample to be tested, ensuring that the sample to be tested passes sequentially from sample pad 23 through conjugation pad 24, nitrocellulose membrane 25, and finally reaches absorbent pad 28.

[0049] The sample pad 23 corresponds to the sample application hole 4 and is the area where the sample is applied. The sample starts to move from here, passing through the binding pad 24 and the nitrocellulose membrane 25, and finally reaches the absorbent pad 28.

[0050] The conjugate pad 24 contains a marker (such as a gold-labeled antibody or a chromogenic microsphere). When the sample passes through, the marker binds to the target substance in the sample to form a complex, which then moves to the nitrocellulose membrane 25 to react.

[0051] The nitrocellulose membrane 25 is the core reaction area (i.e., reaction display area 21) of the test strip 3. In this area, the sample specifically binds to the marker and target antigen / antibody, forming visible lines.

[0052] Test line 26 is used to detect target antigens or antibodies. It typically contains specific antibodies, which will form a visible line in this area when the target substance is present in the sample, indicating a positive test result.

[0053] Control line 27 is used to verify the validity of the test. It usually includes a control antibody, and control line 27 should be visible regardless of the presence of the target substance in the sample to ensure that the test strip is working properly.

[0054] The absorbent pad 28 is located at the end of the test strip 3 and is used to absorb excess sample liquid to ensure that the sample moves evenly and avoid residues affecting the test results.

[0055] Specifically, the conjugate pad 24 is provided with colorimetric microspheres through physical adsorption, and the number of test lines 26 is at least one.

[0056] In one embodiment, the chromogenic microspheres are provided with monoclonal antibodies against the STD to be tested, monoclonal antibodies against lactobacilli, and IgY antibodies.

[0057] In one embodiment, such as Figure 6As shown, the number of test lines 26 is 1.

[0058] In another embodiment, the number of test lines 26 is 2 or 3.

[0059] In one specific embodiment, there are three test lines 26, namely test line B1, test line B2, and test line B3 along the chromatography direction of the sample to be tested; wherein, the goat anti-chicken polyclonal antibody is coated in the area of ​​test line B3, the area of ​​test line B2 containing the STD test item, and the area of ​​test line B1 containing lactobacillus. This embodiment can simultaneously detect the STD test item and the lactobacillus content to assess the microecological health, with high accuracy and reliability, and the detection process is simple and fast.

[0060] Specifically, the baffle 6 is made of colorless transparent glass or colorless transparent plastic.

[0061] The transparent baffle 6 of this utility model protects sensitive areas while allowing operators to observe results from a specific angle, balancing safety and convenience, and meeting laboratory specifications.

[0062] Specifically, the sample loading port 4 is located directly above the sample pad 23, and the result display area 5 is located directly above the reaction display area 21.

[0063] Specifically, both the upper housing 1 and the lower housing 2 are made of plastic.

[0064] In one embodiment, the upper housing 1 and the lower housing 2 are made of polypropylene or polystyrene.

[0065] This novel rapid microbial detection kit is made of plastic, featuring a lightweight design for easy portability and operation, while reducing transportation costs. The plastic possesses excellent chemical inertness, corrosion resistance, and reagent penetration resistance, maintaining reagent stability over a long period and preventing interference with samples or reaction components. Furthermore, high-precision injection molding technology enables complex structures (such as sample loading holes and limiting slots), ensuring component compatibility and sealing, and reducing the risk of sample leakage. Some plastics (such as transparent polypropylene) also have good light transmittance, facilitating observation of colorimetric reactions, and support high-temperature sterilization or radiation disinfection, meeting aseptic testing requirements. In addition, the low cost of plastic materials allows for mass production, meets the hygiene requirements for disposable consumables, effectively avoids cross-contamination, and improves the safety and reliability of the test.

[0066] The technical solution of this utility model has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the protection scope of this utility model is obviously not limited to these specific embodiments. Without departing from the principle of this utility model, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the protection scope of this utility model.

[0067] The above description is merely a preferred embodiment of this utility model and is not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A rapid microbial detection kit, characterized by, The device comprises an upper shell, a lower shell and a test strip, the lower shell is internally provided with a clamping groove for accommodating the test strip, the test strip is arranged in the lower shell, the upper shell is provided with a sample adding hole and a result display area, the result display area is provided with a baffle, and one side of the upper surface of the baffle is provided with a colorimetric area.

2. The rapid microbial test kit according to claim 1, characterized in that, The bottom surface of the result display area is lower than the upper surface of the upper shell to form a recessed area, the result display area is provided with a first window and a second window, the length of the baffle is less than the length of the recessed area and greater than the distance between the two distal ends of the first window and the second window, and the baffle is embedded in the recessed area to slide in the recessed area.

3. The rapid microbial test kit according to claim 1, wherein The sample adding hole comprises an upper port and a lower port, the opening area of the upper port is greater than that of the lower port, and the upper port and the lower port are connected by an arc surface.

4. The rapid microbial test kit according to claim 1, wherein The clamping groove comprises a supporting structure and a limiting structure, the supporting structure and the limiting structure are fixedly connected with the lower shell, the supporting structure comprises a plurality of supporting strips, and the limiting structure comprises a limiting block and a limiting baffle.

5. The rapid microbial test kit according to claim 1, wherein The lower surface of the upper shell is provided with a plurality of connecting columns, the lower shell is internally provided with a plurality of joints corresponding to the connecting columns, the connecting columns can be inserted into the joints, and the connecting columns and the joints are in interference fit.

6. The rapid microbial test kit according to claim 1, wherein The test strip comprises a sample area, a reaction display area and a water absorption area arranged in sequence along the chromatography direction of the sample to be detected, the sample area comprises a sample pad and a binding pad arranged in sequence along the chromatography direction of the sample to be detected, the reaction display area is provided with a nitrocellulose membrane, the nitrocellulose membrane is provided with a test line and a control line along the chromatography direction of the sample to be detected, and the water absorption area is provided with a water absorption pad.

7. The rapid microbial test kit according to claim 6, wherein The binding pad is provided with color developing microspheres in a physical adsorption manner, and the number of the test line is at least one.

8. The rapid microbial test kit of claim 1, wherein The baffle is made of colorless transparent glass or colorless transparent plastic material.

9. The rapid microbial test kit according to claim 6, wherein The sample adding hole is located directly above the sample pad, and the result display area is located directly above the reaction display area.

10. The rapid microbial test kit according to any one of claims 1 to 9, characterized in that, The upper shell and the lower shell are made of plastic material.

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