High-flux deep hole plate
By designing a detachable high-throughput deep-well plate structure, the problem of the inability to replace culture plates individually was solved, reducing usage costs and improving the dissolved oxygen efficiency of the culture medium.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-12
- Publication Date
- 2026-03-20
AI Technical Summary
The existing culture plates cannot be replaced individually, resulting in high usage costs.
A high-throughput deep well plate was designed, comprising a plate body, a frame, and a cover plate. The plate body and the frame are detachably connected by a locking assembly, and an extension plate is set in the culture well to increase the contact area between the liquid and the air.
The plate can be replaced individually, reducing the cost of use, and the oxygenation efficiency of the culture medium is improved by extending the plate.
Smart Images
Figure CN224015616U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture plate technical field especially, relate to a kind of high-throughput deep well plate. BACKGROUND
[0002] Cell culture plate can utilize less culture solution to carry out cell high-throughput culture and strain or cell strain screening, with the characteristics of fast, high-throughput, cost-saving etc., in the process of cell culture, generally first using cell culture plate to carry out high-throughput, small volume cell culture, and the optimal strain is screened out, and the better process culture condition is preliminarily determined. Then the screened strain and the preliminarily determined process condition are applied to fermenter, and scale-up culture is carried out.
[0003] The culture plate in prior art is mostly integrally injection molded, provided with shell and top cover, so that when the culture plate needs to be replaced, the user needs to replace the culture plate and the shell together, causing high culture plate use cost.
[0004] Therefore, the culture plate in prior art has the technical problem of being unable to be replaced individually. SUMMARY
[0005] The high-throughput deep well plate provided by the utility model solves the technical problem that the culture plate in prior art cannot be replaced individually.
[0006] Some embodiments for solving the above technical problem include:
[0007] A high-throughput deep well plate, comprising a plate body, wherein the plate body is uniformly provided with culture holes;
[0008] a frame, wherein the frame is sleeved on the plate body;
[0009] and a cover plate, wherein the cover plate is sleeved on the frame;
[0010] The frame is provided with a locking assembly for locking the plate body to the frame, and the cover plate pushes the locking assembly to lock the plate body to the frame.
[0011] The cover plate is provided with an extension plate, and the extension plate extends into the culture hole.
[0012] Preferably, the extension plate has a polygonal cross-sectional shape, or the extension plate comprises a longitudinal plate and a transverse plate perpendicular to the longitudinal plate.
[0013] Preferably, the cover plate comprises a top plate, the extension plate and the top plate are in an integral structure, and the cover plate further comprises a side plate forming a cylindrical shape, and the top plate and the side plate are in an integral structure.
[0014] As preferred, the locking assembly comprises a sliding plate connected to the frame, the plate body is provided with a locking groove matched with the sliding plate, the frame is provided with a sliding hole guiding the sliding plate, and the sliding hole penetrates the side wall of the frame.
[0015] As preferred, the sliding plate is provided with a driving plate, the driving plate is perpendicular to the sliding plate, and the driving plate is in an integral structure with the sliding plate, the driving plate is provided with a driving surface in contact with the cover plate, the driving surface is obliquely arranged, and the cover plate extends the sliding plate into the locking groove through the driving surface.
[0016] As preferred, the frame is further provided with a containing groove, the containing groove is matched with the driving plate, and the containing groove is communicated with the sliding groove.
[0017] As preferred, the locking assembly further comprises an elastic member, one end of the elastic member is fixed to the driving plate, and the other end of the elastic member is arranged corresponding to the bottom wall of the containing groove.
[0018] As preferred, the elastic member is arranged on both sides of the sliding plate, the elastic member is a spring, and the elastic member is bonded to the driving plate.
[0019] As preferred, the locking assembly is two, and the two locking assemblies are arranged on opposite sides of the frame.
[0020] As preferred, the diameter of the circumscribed circle of the extension plate is smaller than the diameter of the inscribed circle of the culture hole, and the length of the extension plate is smaller than the depth of the culture hole.
[0021] Compared with the prior art, the utility model has the following advantages:
[0022] Through the arrangement of the frame, the cover plate and the locking assembly, the cover plate pushes the locking assembly to lock the plate body on the frame. When it is needed to fix the plate body on the frame, the locking assembly can conveniently lock the plate body on the frame, when it is needed to replace the plate body, the locking assembly can be loosened to unlock the plate body from the frame, so that the plate body can be conveniently replaced, and the plate body can be replaced individually, thereby reducing the use cost of the culture plate.
[0023] Through the arrangement of the extension plate, the extension plate extends into the culture hole, in the working process of the culture hole, the culture liquid in the culture hole interferes with the extension plate, so that the culture liquid forms a turbulent flow in the culture hole, so that the culture liquid has a larger contact area with air, thereby making the culture hole have a reasonable oxygen coefficient. BRIEF DESCRIPTION OF DRAWINGS
[0024] For purposes of explanation, several embodiments of the subject technology are set forth in the following Figures. The Figures were incorporated herein and constitute a part of the detailed description. In some instances, well-known structures and components were shown in block diagram form in order to avoid obscuring the concept of the subject technology.
[0025] Fig. 1 is a schematic view of the internal structure of the present application.
[0026] Fig. 2 is a schematic view of the present application.
[0027] Fig. 3 is Fig. 2 is a schematic view after omitting the cover plate.
[0028] Fig. 4 is an exploded view of the present application.
[0029] Fig. 5 is a schematic view of the cover plate.
[0030] Fig. 6 is a schematic view of the locking assembly.
[0031] shown in the Figures:
[0032] 1, plate body, 11, culture hole, 12, locking groove, 2, frame, 21, sliding plate, 22, driving plate, 221, driving surface, 23, elastic member, 3, cover plate, 31, extension plate, 32, top plate, 33, side plate. DETAILED DESCRIPTION
[0033] The specific embodiments shown are intended to be illustrative of the various configurations of the subject technology and are not intended to limit the scope of the subject technology to these specific embodiments. The specific embodiments include specific details to provide a thorough understanding of the subject technology. However, it will be apparent to those skilled in the art that the subject technology can be practiced without these specific details.
[0034] It can be understood that, in this document, relational terms such as "first" and "second", and the like, are used solely to distinguish one entity or action from another, without necessarily implying any actual relationship or order between such entities or actions.
[0035] The terms "comprise", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element preceded by "comprises a" does not, without more constraints, foreclose the existence of additional identical elements in the process, method, article, or apparatus that comprises the recited element.
[0036] Referring to Figs. 1 to 6 As shown in the drawings, a high-throughput deep-well plate comprises a plate body 1, wherein the plate body 1 is uniformly provided with culture wells 11;
[0037] a frame 2, wherein the frame 2 is sleeved on the plate body 1;
[0038] and a cover plate 3, wherein the cover plate 3 is sleeved on the frame 2;
[0039] The frame 2 is provided with a locking assembly for locking the plate body 1 to the frame 2, and the cover plate 3 pushes the locking assembly to lock the plate body 1 to the frame 2;
[0040] The cover plate 3 is provided with an extension plate 31, wherein the extension plate 31 extends into the culture well 11.
[0041] It can be understood that, due to the provision of the extension plate 31, the extension plate 31 extends into the culture well 11, and when the culture solution in the culture well 11 is vibrated, the culture solution contacts the extension plate 31, the extension plate 31 can effectively disperse the culture solution, so that the culture solution has a larger contact area with air, thereby making the culture plate have a more reasonable dissolved oxygen coefficient, so that the culture well 11 can be provided with a deeper depth.
[0042] In addition, the plate body 1 may be damaged or need to be replaced during use, and the locking assembly is used to lock the plate body 1 to the frame 2, and the locking assembly can conveniently lock or release the plate body 1, so that the plate body 1 can be independently replaced.
[0043] Since the plate body 1 can be independently replaced, the use cost of the culture plate is reduced.
[0044] In some embodiments, the extension plate 31 has a polygonal cross-sectional shape, or the extension plate 31 comprises a longitudinal plate and a transverse plate perpendicular to the longitudinal plate.
[0045] The cross-sectional shape of the extension plate 31 should not be circular, i.e. the surface of the extension plate 31 should have a non-smooth surface such as an edge line, so that the extension plate 31 can guide the culture solution when the culture solution contacts the extension plate 31, and thus the culture solution does not flow along the side wall of the culture hole 11, and thus the culture solution has a larger contact area with air, so that the plate body 1 has a reasonable dissolved oxygen coefficient.
[0046] Referring to Figs. 1 to 6 As shown in the drawings, in some embodiments, the cover plate 3 comprises a top plate 32, the extension plate 31 and the top plate 32 are in an integral structure, and the cover plate 3 further comprises a side plate 33 surrounding a cylinder, and the top plate 32 and the side plate 33 are in an integral structure. The top plate 32 and the side plate 33 can be formed by injection molding.
[0047] In some embodiments, the locking assembly comprises a sliding plate 21 slidably connected to the frame 2, the plate body 1 is provided with a locking groove 12 matched with the sliding plate 21, and the frame 2 is provided with a sliding hole guiding the sliding plate 21, and the sliding hole penetrates the side wall of the frame 2.
[0048] In some embodiments, the sliding plate 21 is provided with a driving plate 22, the driving plate 22 is perpendicular to the sliding plate 21, and the driving plate 22 and the sliding plate 21 are in an integral structure, the driving plate 22 is provided with a driving surface 221 in contact with the cover plate 3, the driving surface 221 is obliquely arranged, and the cover plate 3 extends the sliding plate 21 into the locking groove 12 through the driving surface 221.
[0049] In some embodiments, the frame 2 is further provided with a containing groove, the containing groove is matched with the driving plate 22, and the containing groove is communicated with the sliding groove.
[0050] In some embodiments, the locking assembly further comprises a resilient member 23, one end of the resilient member 23 is fixed to the driving plate 22, and the other end of the resilient member 23 is arranged corresponding to the bottom wall of the containing groove.
[0051] In some embodiments, the sliding plate 21 is provided with the resilient member 23 on both sides, the resilient member 23 is a spring, and the resilient member 23 is bonded to the driving plate 22. The locking assembly has two, and the two locking assemblies are arranged on opposite sides of the frame 2.
[0052] Specifically, the elastic member 23 can be fixed to the bottom wall of the accommodating groove, or can not be fixed to the bottom wall of the accommodating groove. The cover plate 3 is sleeved on the frame 2 from top to bottom. In the assembly process, first, the plate body 1 is installed with the frame 2, that is, the plate body 1 is inserted into the frame 2. Then, the locking assembly is installed in the sliding groove, at this time, under the action of the elastic member 23, the sliding plate 21 does not extend into the locking groove 12, and the plate body 1 and the frame 2 are in a disengaged and unlocked state.
[0053] When the cover plate 3 is sleeved on the frame 2, under the action of the elastic member 23, the driving plate 22 protrudes from the side wall of the frame 2. When the cover plate 3 moves downward, the side plate 33 is in contact with the driving surface 221 of the driving plate 22, and the driving surface 221 divides the force applied by the side plate 33 into two components, one of which overcomes the force of the elastic member 23, so that the sliding plate 21 extends into the locking groove 12, and at this time, the plate body 1 and the frame 2 are in a locked state.
[0054] When the cover plate 3 is separated from the frame 2, under the action of the elastic member 23, the sliding plate 21 automatically slides out of the locking groove 12, so that the plate body 1 can be conveniently replaced.
[0055] Referring to Figs. 1 to 6 As shown in the drawings, in some embodiments, the diameter of the circumscribed circle of the extension plate 31 is less than the diameter of the inscribed circle of the culture well 11, and the length of the extension plate 31 is less than the depth of the culture well 11.
[0056] The diameter of the extension plate 31 is not limited, and there should be a gap between the extension plate 31 and the culture well 11 to prevent the extension plate 31 from dividing the culture solution into multiple non-communicating areas.
[0057] The above describes the subject technical solution of the utility model and the corresponding details, and it can be understood that the above description is only some implementation of the subject technical solution of the utility model, and some details can be omitted in the specific implementation.
[0058] In addition, in some embodiments of the above utility model, multiple embodiments can be combined for implementation, and various combination solutions are not listed one by one due to the length. Those skilled in the art can freely combine the above embodiments according to the needs in the specific implementation to obtain better application experience.
[0059] Those skilled in the art can obtain other detailed configurations or drawings according to the subject technical solution of the utility model and the drawings when implementing the subject technical solution of the utility model, and it is obvious that these details still belong to the scope covered by the subject technical solution of the utility model without departing from the subject technical solution of the utility model.
Claims
1. A high-throughput deep-hole plate, characterized in that: The device includes a plate (1) with culture wells (11) evenly distributed; a frame (2) fitted over the plate (1); and a cover plate (3) fitted over the frame (2); the frame (2) is provided with a locking assembly for locking the plate (1) to the frame (2), and the cover plate (3) pushes the locking assembly to lock the plate (1) to the frame (2); the cover plate (3) is provided with an extension plate (31) extending into the culture wells (11).
2. The high-throughput deep hole plate according to claim 1, characterized in that: The extension plate (31) has a polygonal cross-sectional shape, or the extension plate (31) includes a longitudinal plate and a transverse plate perpendicular to the longitudinal plate.
3. The high-throughput deep hole plate according to claim 2, characterized in that: The cover plate (3) includes a top plate (32), the extension plate (31) and the top plate (32) are an integral structure, and the cover plate (3) also includes a side plate (33) forming a cylinder, the top plate (32) and the side plate (33) are an integral structure.
4. The high-throughput deep hole plate according to claim 1, characterized in that: The locking assembly includes a sliding plate (21) slidably connected to the frame (2), the plate body (1) is provided with a locking groove (12) that cooperates with the sliding plate (21), and the frame (2) is provided with a sliding hole that guides the sliding plate (21) through the side wall of the frame (2).
5. The high-throughput deep hole plate according to claim 4, characterized in that: The slide plate (21) is provided with a drive plate (22), which is perpendicular to the slide plate (21). The drive plate (22) and the slide plate (21) are an integral structure. The drive plate (22) is provided with a drive surface (221) that contacts the cover plate (3). The drive surface (221) is inclined. The cover plate (3) extends the slide plate (21) into the locking groove (12) through the drive surface (221).
6. The high-throughput deep hole plate according to claim 5, characterized in that: The frame (2) is also provided with a receiving groove, which cooperates with the drive plate (22) and is connected to the slide groove.
7. The high-throughput deep hole plate according to claim 6, characterized in that: The locking assembly also includes an elastic element (23), one end of which is fixed to the drive plate (22), and the other end of which is correspondingly disposed to the bottom wall of the receiving groove.
8. The high-throughput deep hole plate according to claim 7, characterized in that: Both sides of the slide plate (21) are provided with elastic elements (23), which are springs and are bonded to the drive plate (22).
9. The high-throughput deep hole plate according to claim 8, characterized in that: There are two locking components, which are respectively disposed on opposite sides of the frame (2).
10. The high-throughput deep hole plate according to claim 1, characterized in that: The outer diameter of the extension plate (31) is smaller than the inner diameter of the culture hole (11), and the length of the extension plate (31) is smaller than the depth of the culture hole (11).