Assembled cell sieve tube

Abstract

The utility model discloses an assembled cell sieve tube which comprises a sealing cover, an upper-layer liquid adding tube, a first-layer cell sieve and a lower-layer collecting tube which are sequentially arranged from top to bottom, wherein the sealing cover is detachably connected with the upper part of the upper-layer liquid adding pipe; the upper part of the first-layer cell screen is detachably connected with the lower part of the upper-layer liquid adding pipe; and the upper part of the lower-layer collecting pipe is detachably connected with the lower part of the upper-layer liquid adding pipe. The filter has the beneficial effects that the filter can be matched with a centrifugal machine for use, and the filtering speed is increased; after centrifugal filtration is finished, the first-layer cell screen can be selectively detached, and a new screen is replaced for centrifugal filtration again; once blockage occurs, the blockage problem can be solved through oscillation resuspension, and the operation is simple and convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical instrument technical field especially relates to an assembled cell screen pipe. BACKGROUND

[0002] Cell screen is generally used in cell culture, impurity filtration, cell dispersion, dispersion and the like, and is a simple and easy method for obtaining single cell suspension.

[0003] The conventional cell screen is composed of a polypropylene frame and a nylon screen, and the screen has three specifications with pore diameters of 40, 70 and 100 um, is independently packaged, and is used in combination with a 50 ml centrifuge tube after irradiation sterilization.

[0004] When the single-layer screen is used, the screen is placed at the mouth of the 50 ml centrifuge tube, and the cell liquid to be filtered is poured into the screen;

[0005] When the multi-layer screen is used, the first layer of screen is installed at the mouth of the 50 ml centrifuge tube through screw threads or buckles, the second layer of screen is installed on top of the first layer of screen, the third layer of screen is installed on top of the second layer of screen, and so on; and after the installation is completed, the cell liquid is poured into the topmost screen.

[0006] The flow of the cell liquid can be accelerated through centrifugation or appropriate pressurization, and after the filtration is completed, the required part is selected or the required cells are brushed off from the screen and collected into another centrifuge tube.

[0007] The single-layer cell screen and the multi-layer cell screen in the prior art have the following disadvantages:

[0008] (1) The single-layer cell screen has the following disadvantages (as shown in Figure 1 ):

[0009] ① The structure is simple, and the 50 ml centrifuge tube cannot be tightly combined with the mouth, which is easy to fall off;

[0010] ② The cell cannot be sealed, and the probability of contamination is large;

[0011] ③ The cell liquid can only be slowly added, and cannot be added in large quantities at one time;

[0012] ④ Only gravity static filtration can be used, and if the cell group is large and the viscosity is large, it is easy to block.

[0013] (2) The multi-layer cell screen has the following disadvantages (as shown in Figure 2 , 3 ):

[0014] ① The screen is fixed on the top of the 50 ml centrifuge tube through the snap ring, and cannot be sealed;

[0015] ② The 50 ml centrifuge cannot be used for centrifugal filtration to speed up the filtration speed;

[0016] ③Multi-layer superposition, filter screen between small space, also can not be a large number of cell liquid filter at one time;

[0017] ④If the cell mass is more or more viscous cell liquid, it is difficult to determine which layer when the blockage occurs, and the disassembly is easy to cause pollution;

[0018] ⑤When the screen is blocked, the cell liquid cannot be resuspended by shaking to speed up the filtration.

[0019] In summary, the utility model discloses an assembled cell screen pipe which can overcome the shortcomings in the prior art: single-layer filtration and multi-layer filtration can be flexibly selected, and the volume of the collection pipe can be flexibly selected; when there is only single-layer or double-layer screen, the filtration speed can be accelerated by centrifugation. Utility model content

[0020] The utility model discloses a cell sorting separation pipe set which is proposed to solve the shortcomings in the prior art.

[0021] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0022] An assembled cell screen pipe comprises a sealing cover, an upper layer liquid adding pipe, a first layer cell screen and a lower layer collection pipe which are sequentially arranged from top to bottom.

[0023] The sealing cover is detachably connected to the upper part of the upper layer liquid adding pipe.

[0024] The upper part of the first layer cell screen is detachably connected to the lower part of the upper layer liquid adding pipe.

[0025] The upper part of the lower layer collection pipe is detachably connected to the lower part of the upper layer liquid adding pipe.

[0026] As a further preferred scheme, the sealing cover is detachably connected to the upper part of the upper layer liquid adding pipe through threads.

[0027] The upper part of the first layer cell screen is detachably connected to the lower part of the upper layer liquid adding pipe through threads.

[0028] The upper part of the lower layer collection pipe is detachably connected to the lower part of the upper layer liquid adding pipe through threads.

[0029] As a further preferred scheme, the inner side of the ring wall of the sealing cover is provided with internal threads.

[0030] As a further preferred scheme, the outer side of the ring wall of the upper layer liquid adding pipe is provided with external threads at the upper end part and the lower end part, and the inner side of the upper layer liquid adding pipe is further provided with internal threads at the lower end part.

[0031] As a further preferred solution, the upper outer end of the first layer cell screen is provided with external threads.

[0032] As a further preferred solution, the upper inner end of the lower layer collecting tube is provided with internal threads.

[0033] As a further preferred solution, a combined functional unit is further included, which is detachably connected with the upper layer liquid feeding tube;

[0034] The number of the combined functional units is a, wherein a satisfies the number relationship: a≥1.

[0035] As a further preferred solution, when a=1, the assembled cell screen is a double-layer cell screen;

[0036] When a=2, the assembled cell screen is a three-layer cell screen.

[0037] As a further preferred solution, the combined functional unit includes a middle layer liquid feeding tube and a second layer cell screen, wherein the middle layer liquid feeding tube is detachably connected with the second layer cell screen, and the middle layer liquid feeding tube is detachably connected with the lower layer collecting tube.

[0038] As a further preferred solution, the upper end of the middle layer liquid feeding tube is provided with internal threads matched with the upper layer liquid feeding tube;

[0039] The lower end of the middle layer liquid feeding tube is provided with internal threads matched with the second layer cell screen;

[0040] The lower end of the middle layer liquid feeding tube is provided with internal threads matched with the lower layer collecting tube.

[0041] The multi-layer cell screen has the following advantages compared with conventional single-layer and multi-layer screens:

[0042] 1. Single-layer filtration and multi-layer filtration can be flexibly selected, and the volume of the collecting tube can be flexibly selected;

[0043] 2. Multi-layer cell separation can be simultaneously realized in a single tube;

[0044] 3. When there is only a single-layer or double-layer screen, the filtration speed can be accelerated through centrifugation;

[0045] 4. When blockage occurs, the blockage position is clear, and the blockage problem can be solved by oscillating and resuspending the cells;

[0046] 5. The bottom collecting tube is detachable, and it is simpler to obtain a single cell suspension;

[0047] 6. The top internal threads of the three-threaded middle layer liquid feeding tube can be combined with other bottom external threads, the tube body can be increased according to requirements, and a larger volume of cell liquid can be filtered at one time;

[0048] 7, three thread middle layer liquid adding pipe and cell screen form combined functional unit as a kit, can be freely stacked and assembled;

[0049] 8, the utility model discloses a multilayer cell screen pipe, can flexibly select single layer filtration and multilayer filtration, realizes multilayer cell separation in single pipe. BRIEF DESCRIPTION OF DRAWINGS

[0050] Figure 1 It is a perspective view of the assembled cell screen pipe (double layer) of the utility model;

[0051] Figure 2 It is a sectional view of the assembled cell screen pipe (double layer) of the utility model; Figure 1 ;

[0052] Figure 3 It is an explosion view of the assembled cell screen pipe (single layer) of the utility model;

[0053] Figure 4 It is an explosion view of the assembled cell screen pipe (double layer) of the utility model;

[0054] Figure 5 It is a perspective view of the sealing cover of the assembled cell screen pipe of the utility model;

[0055] Figure 6 It is a perspective view of the combined functional unit of the assembled cell screen pipe of the utility model;

[0056] Figure 7 It is a perspective view of the lower layer collecting pipe of the assembled cell screen pipe of the utility model;

[0057] Figure 8 It is a sectional view of the assembled cell screen pipe (double layer) of the utility model; Figure 2 ;

[0058] Figure 9 It is a sectional view of the assembled cell screen pipe (three layers) of the utility model; Figure 1 ; DETAILED DESCRIPTION

[0059] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0060] With reference to Figures 1-5 , the utility model provides a kind of assembled cell screen pipe.

[0061] Example 1 (single layer)

[0062] An assembled cell screen pipe comprises, from top to bottom, a sealing cover 10, an upper layer liquid feeding pipe 20, a first layer cell screen 30, and a lower layer collecting pipe 60;

[0063] The sealing cover 10 is detachably connected to the upper part of the upper layer liquid feeding pipe 20.

[0064] The upper part of the first layer cell screen 30 is detachably connected to the lower part of the upper layer liquid feeding pipe 20.

[0065] The upper part of the lower layer collecting pipe 60 is detachably connected to the lower part of the upper layer liquid feeding pipe 20.

[0066] As described in the background, both the single layer cell screen and the multi-layer cell screen have many shortcomings:

[0067] 1) easy to be contaminated;

[0068] 2) not compatible with a centrifuge;

[0069] 3) the screen is easy to be blocked, and the cell liquid cannot be resuspended by shaking to speed up the filtration.

[0070] In this embodiment, the assembled cell screen pipe can overcome the above two shortcomings, so that the cell liquid can be directly filtered; if blocked, the cell liquid can be resuspended by shaking to speed up the filtration; further, the centrifuge can be used to speed up the filtration speed.

[0071] In this embodiment, the single layer assembled cell screen pipe has the following working principle:

[0072] In use, only the uppermost sealing cover 10 is opened, and the prepared cell liquid is poured in, so that the cell liquid is filtered through the first layer cell screen 30, and the required part of the cell is collected after the filtration is completed.

[0073] If the concentration of the cell liquid to be filtered is high and the cell liquid contains many clumps, the centrifuge can be used to speed up the filtration speed in the case of installing the first layer cell screen 30 (single layer screen); after the centrifugal filtration is completed, the first layer cell screen 30 can be selectively disassembled, and a new screen is replaced for centrifugal filtration again; if blocked, the problem of blockage can be solved by resuspension by shaking, which is simple and convenient to operate.

[0074] Example 2 (single layer)

[0075] In this embodiment:

[0076] The sealing cover 10 is detachably connected to the upper part of the upper layer liquid feeding pipe 20 by threads;

[0077] The upper part of the first layer cell screen 30 is detachably connected with the lower part of the upper layer liquid adding pipe 20 through screw thread;

[0078] The upper part of the lower layer collecting pipe 60 is detachably connected with the lower part of the upper layer liquid adding pipe 20 through screw thread.

[0079] More specifically:

[0080] The inner side of the ring wall of the sealing cover 10 is provided with internal thread;

[0081] The outer side of the upper end of the ring wall of the upper layer liquid adding pipe 20 is provided with external thread (which cooperates with the internal thread of the sealing cover 10), and the outer side of the lower end of the ring wall of the upper layer liquid adding pipe 20 is provided with external thread, and the inner side of the lower end of the upper layer liquid adding pipe 20 is also provided with internal thread;

[0082] The outer side of the upper part of the first layer cell screen 30 is provided with external thread (which cooperates with the internal thread of the inner side of the lower end of the upper layer liquid adding pipe 20);

[0083] The inner side of the upper part of the lower layer collecting pipe 60 is provided with internal thread (which cooperates with the external thread of the outer side of the lower end of the ring wall of the upper layer liquid adding pipe 20).

[0084] Through the above specific structure, the sealing cover 10, the upper layer liquid adding pipe 20, the first layer cell screen 30, and the lower layer collecting pipe 60 can be sealed through the rotation of the components, thereby facilitating the next step of cell liquid filtration operation.

[0085] Example 2 (single layer)

[0086] In this embodiment

[0087] 1) The outer side of the sealing cover 10 is provided with a plurality of strip-shaped anti-slip protrusions 11 arranged in the vertical direction.

[0088] The strip-shaped anti-slip protrusions can improve the holding stability, prevent accidental slipping, increase the friction, and facilitate rotation;

[0089] 2) The lower layer collecting pipe 60 includes a collecting cavity 61 and a base 62, and the base is arranged at the bottom of the lower layer collecting pipe 60.

[0090] Example 3 (double layer, triple layer, or even more layers)

[0091] In this embodiment, a combined functional unit 40 is also included, which is detachably connected with the upper layer liquid adding pipe 20;

[0092] The number of the combined functional unit 40 is a, wherein a satisfies the number relationship: a≥1.

[0093] When a = 1, the assembled cell screen pipe is a double layer cell screen pipe;

[0094] When a = 2, the assembled cell screen pipe is a three-layer cell screen pipe.

[0095] As a further preferred solution:

[0096] The combined functional unit 40 comprises a middle layer liquid feeding pipe 41 and a second layer cell screen 42, wherein the middle layer liquid feeding pipe 41 is detachably connected with the second layer cell screen 42, and the middle layer liquid feeding pipe 41 is detachably connected with the lower layer collecting pipe 60.

[0097] More specifically:

[0098] The inner side of the upper end of the middle layer liquid feeding pipe 41 is provided with an internal thread matched with the upper layer liquid feeding pipe 20;

[0099] The inner side of the lower end of the middle layer liquid feeding pipe 41 is provided with an internal thread matched with the second layer cell screen 42;

[0100] The outer side of the lower end of the middle layer liquid feeding pipe 41 is provided with an internal thread matched with the lower layer collecting pipe 60.

[0101] According to the above structure, different numbers of combined functional units 40 can be set according to specific needs.

[0102] The multi-layer cell screen pipe in the embodiment comprises a sealing cover 10, an upper layer liquid feeding pipe 20, a first layer cell screen 30, a combined functional unit 40 (a middle layer liquid feeding pipe 41 and a second layer cell screen 42), and a lower layer collecting pipe 60.

[0103] The middle layer liquid feeding pipe 41 and the second layer cell screen 42 are a sleeve, i.e. the combined functional unit 40, which can be assembled in multiple layers through threads.

[0104] Specific operation mode: the bottom internal thread of the middle layer liquid feeding pipe 41 is combined with the top external thread of the cell screen; another middle layer liquid feeding pipe 41 is combined with the bottom external thread of the middle layer liquid feeding pipe 41 installed with the cell screen.

[0105] After multiple layers are stacked according to the number of screens required, the lower layer collecting pipe 60 is installed at the bottom, the upper layer liquid feeding pipe 20 and the sealing cover 10 are installed at the top, and a complete multi-layer cell screen pipe is formed.

[0106] When used, only the uppermost sealing cover 10 needs to be opened, and the prepared cell liquid is poured in, so that the filtration can be performed, and after the filtration is completed, the required part of the cells can be collected.

[0107] According to the requirement, the screen with different aperture can be installed, such as 100um, 70um, 40um cell screen from top to bottom, which can filter out the cell group, tissue block or impurities, etc. in layers, avoid the blockage, and obtain the single cell suspension with single shape and function.

[0108] If the concentration of the cell liquid to be filtered is high and the cell group is much, the single layer or double layer screen can be installed to match the centrifuge, and the filtering speed is accelerated. After the centrifugal filtration is finished, the screen can be selectively disassembled, the new screen is replaced, and the centrifugal filtration is performed again. Once the blockage occurs, the blockage problem can be solved by oscillation resuspension.

[0109] Finally, it should be noted that the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can be modified to the technical solution recorded in the foregoing embodiments, or equivalent replacement for part of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, should be included in the protection scope of the present application.

Claims

1. An assembled cell sieve tube, characterized in that, It includes, from top to bottom, a sealing cap (10), an upper liquid addition tube (20), a first layer of cell sieve (30), and a lower collection tube (60); The sealing cap (10) is detachably connected to the upper part of the upper liquid filling tube (20); The upper part of the first layer cell sieve (30) is detachably connected to the lower part of the upper liquid filling tube (20); The upper part of the lower collection tube (60) is detachably connected to the lower part of the upper liquid addition tube (20).

2. The assembled cell sieve tube according to claim 1, characterized in that, The sealing cap (10) is detachably connected to the upper part of the upper liquid filling tube (20) by threads; The upper part of the first layer cell sieve (30) is detachably connected to the lower part of the upper liquid filling tube (20) by threads; The upper part of the lower collection tube (60) is detachably connected to the lower part of the upper liquid addition tube (20) by threads.

3. The assembled cell sieve tube according to claim 1, characterized in that, The inner side of the ring wall of the sealing cap (10) is provided with internal threads.

4. The assembled cell sieve tube according to claim 1, characterized in that, The upper end of the outer side of the ring wall of the upper liquid filling pipe (20) is provided with an external thread, and the lower end of the outer side of the ring wall of the upper liquid filling pipe (20) is provided with an external thread, and the lower end of the inner side of the upper liquid filling pipe (20) is also provided with an internal thread.

5. The assembled cell sieve tube according to claim 1, characterized in that, The upper outer end of the first layer of cell sieve (30) is provided with external threads.

6. The assembled cell sieve tube according to claim 1, characterized in that, The upper inner side of the lower collection tube (60) is provided with an internal thread.

7. The assembled cell sieve tube according to claim 1, characterized in that, It also includes a modular functional unit (40) which is detachably connected to the upper liquid filling pipe (20); The number of combined functional units (40) is set to a, where a satisfies the quantitative relationship: a≥1.

8. The assembled cell sieve tube according to claim 7, characterized in that, When a = 1, the assembled cell sieve tube is a double-layered cell sieve tube; When a = 2, the assembled cell sieve tube is a three-layer cell sieve tube.

9. The assembled cell sieve tube according to claim 7, characterized in that, The modular functional unit (40) includes a middle layer liquid filling tube (41) and a second layer cell sieve (42), wherein the middle layer liquid filling tube (41) and the second layer cell sieve (42) are detachably connected, and the middle layer liquid filling tube (41) and the lower layer collection tube (60) are detachably connected.

10. The assembled cell sieve tube according to claim 7, characterized in that, The upper end of the middle layer liquid filling tube (41) is provided with an internal thread that mates with the upper layer liquid filling tube (20); The lower end of the middle layer liquid filling tube (41) is provided with an internal thread that mates with the second layer cell sieve (42); The lower end of the middle layer liquid addition tube (41) is provided with an internal thread that mates with the lower layer collection tube (60).

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