Antigen purification and concentration equipment

Abstract

The utility model discloses antigen purification and concentration equipment and relates to the technical field of vaccine production. The device comprises a bottom plate, supports are fixedly arranged on the upper surface of the bottom plate, a reaction tank is fixedly arranged between the supports, a tank cover is installed on the reaction tank, a driving motor is fixedly arranged on the upper surface of the tank cover, a first connecting rod is arranged at the output end of the driving motor, the first connecting rod penetrates through the tank cover, and a second connecting rod is arranged at the output end of the driving motor and penetrates through the tank cover. According to the cell sap filtering device, cell sap is filtered through a filtering membrane in the filtering plate, in order to prevent the filtering membrane from being blocked, a second connecting rod can be made to rotate by starting a first motor, the second connecting rod drives a half-face gear to rotate, and the half-face gear drives an annular toothed plate meshed with the half-face gear to move in a reciprocating mode; therefore, the upper end of the ejector rod is in reciprocating contact with the lower surface of the sliding plate, so that the filter plate vibrates, a filter membrane can be prevented from being blocked, and the filter efficiency can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the vaccine production technical field, concretely is an antigen purification concentration equipment. BACKGROUND

[0002] Many non-purpose products such as serum, cell fragments, bacterial bodies and microbial lysates are inevitably produced in the large-scale culture expansion process of vaccine antigens, and these substances are not completely removed in the later stage of vaccine manufacturing or cause immune inoculation side reactions such as allergic reactions.

[0003] The application number is "CN201920678647.8" a kind of high-efficiency vaccine concentration purification device, including ladder, inlet, liquid storage bucket, stirring device, filter mechanism, pressure gauge, controller, pneumatic control cabinet, mounting rack, embedded plate and cylinder, the setting of the utility model magnetic stir bar, set crushing and stirring integrally effectively improve the cell crushing effect, more can make the mixture of cell liquid and venom;The setting of pneumatic control cabinet and filter mechanism, cell liquid and cell fragments are separated using air pressure, effectively increase the filtration effect of cell liquid;The setting of cylinder, embedded plate and filter mechanism, it is convenient to clean or replace filter membrane on cell fragments on filter membrane, effectively realize the integrated setting of equipment, reduce production cost.

[0004] The above-mentioned equipment is fixed on the membrane disc due to the fixed position setting, so that the filter membrane placed on the membrane disc is also fixed with the position, which leads to the cell fragments produced under long-time filtration to easily block the filter membrane, thereby reducing the filtration efficiency of the filter membrane, and in order to accelerate the mixing efficiency by stirring rod to accelerate the mixing, but after use, it is not convenient to disassemble and clean, and then it is easy to affect subsequent production. Utility model content

[0005] In order to solve the problem that the above-mentioned equipment is fixed on the membrane disc due to the fixed position setting, so that the filter membrane placed on the membrane disc is also fixed with the position, which leads to the cell fragments produced under long-time filtration to easily block the filter membrane, thereby reducing the filtration efficiency of the filter membrane, and in order to accelerate the mixing efficiency by stirring rod to accelerate the mixing, but after use, it is not convenient to disassemble and clean, and then it is easy to affect subsequent production;The utility model aims at providing an antigen purification concentration equipment.

[0006] To solve the above technical problems, the present invention adopts the following technical solution: an antigen purification and concentration device, including a base plate, a support fixedly mounted on the upper surface of the base plate, a reaction vessel fixedly mounted between the supports, a lid mounted on the reaction vessel, a drive motor fixedly mounted on the upper surface of the lid, a first connecting rod mounted at the output end of the drive motor, the first connecting rod penetrating the lid, a stirring shaft connected to the first connecting rod, a disassembly assembly connected to the first connecting rod and the stirring shaft, a filter plate slidably mounted inside the reaction vessel, an anti-clogging assembly inside the reaction vessel, the anti-clogging assembly connected to the filter plate, a fixing block fixedly mounted on the lower surface of the lid, a sleeve fixedly mounted on the outer surface of the lid, the fixing block movably inserted into the sleeve, a first bolt threaded into the side of the sleeve, the first bolt movably penetrating the fixing block;

[0007] The anti-clogging component includes a chute formed on the inner surface of the reaction vessel. A spring is fixedly mounted on the inner surface of the chute, and a sliding plate is fixedly mounted on the upper end of the spring. The sliding plate slides against the inner surface of the chute and is fixedly connected to a filter plate. A rectangular through-slot is formed inside the reaction vessel and communicates with the chute. A fixing plate is fixedly mounted on the inner surface of the rectangular through-slot, and a first motor is fixedly mounted on the upper surface of the fixing plate. A second connecting rod is provided at the output end of the first motor, and a half-face gear is fixedly sleeved on the outer surface of the second connecting rod. A rectangular groove is formed on the inner surface of the rectangular through-slot, and a rectangular block slides within the rectangular groove. An annular toothed plate is fixedly mounted on one side of the rectangular block, and the half-face gear meshes with the annular toothed plate. A push rod is fixedly mounted on the upper surface of the annular toothed plate, and the upper end of the push rod is in movable contact with the sliding plate.

[0008] Preferably, the disassembly and assembly assembly includes a positioning block, the upper end of which is fixedly connected to the upper end of the stirring shaft, and the lower end of the first connecting rod is provided with a positioning groove. Both the positioning block and the outer surface of the first connecting rod are provided with through holes, and a second bolt is movably inserted into the through hole. A nut is threaded onto the outer surface of the second bolt.

[0009] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0010] 1. In this invention, cell fluid is filtered through a filter membrane inside a filter plate. To prevent the filter membrane from becoming clogged, the second connecting rod can be rotated by starting the first motor. The second connecting rod drives the half-face gear to rotate, and the half-face gear drives the ring toothed plate meshing with it to move back and forth. This causes the upper end of the push rod to reciprocate to contact the lower surface of the slide plate, thereby causing the filter plate to vibrate. This prevents the filter membrane from becoming clogged and improves the filtration efficiency.

[0011] 2. When the stirring shaft needs to be cleaned, the lid can be removed from the reaction vessel, and then the nut can be turned to remove it from the second bolt. The second bolt can then be pulled out from the through hole, thereby disassembling the stirring shaft for easy cleaning and subsequent production. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the structure of this utility model.

[0014] Figure 2 This is a schematic diagram of the stirring shaft structure of this utility model.

[0015] Figure 3 This is a schematic diagram of the reaction vessel structure of this utility model.

[0016] Figure 4 This utility model Figure 3 Enlarged structural diagram at point A in the middle.

[0017] Figure 5 This is a schematic diagram of the anti-clogging component of this utility model.

[0018] Figure 6 This is a schematic diagram of the filter plate structure of this utility model.

[0019] Figure 7 This utility model Figure 6 Enlarged structural diagram at point B.

[0020] In the diagram: 1. Base plate; 11. Support; 2. Reaction vessel; 21. Discharge pipe; 22. Electric valve; 3. Tank cover; 31. Fixing block; 32. Sleeve; 33. First bolt; 34. Feed pipe; 35. Rotary cap; 4. Drive motor; 41. First connecting rod; 42. Stirring shaft; 5. Assembly / disassembly assembly; 51. Positioning block; 52. Through hole; 53. Positioning groove; 54. Second bolt; 55. Nut; 6. Filter plate; 7. Anti-clogging assembly; 71. Slide groove; 72. Spring; 73. Slide plate; 731. Rectangular through groove; 74. Fixing plate; 741. Waterproof shell; 75. First motor; 76. Second connecting rod; 77. Half-face gear; 78. Rectangular groove; 781. Rectangular block; 79. Annular toothed plate; 791. Top rod. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Example: Figures 1-7 As shown, this utility model provides an antigen purification and concentration device, including a base plate 1. A support 11 is fixedly mounted on the upper surface of the base plate 1. A reaction vessel 2 is fixedly mounted between the support 11. A lid 3 is installed on the reaction vessel 2. A drive motor 4 is fixedly mounted on the upper surface of the lid 3. A first connecting rod 41 is provided at the output end of the drive motor 4, penetrating the lid 3. A discharge pipe 21 is provided on the lower surface of the reaction vessel 2. An electric valve 22 is connected to the discharge pipe 21. A stirring shaft 42 is connected to the first connecting rod 41. The first connecting rod 41 is connected to the stirring shaft 42 by a disassembly assembly 5. After rotating the cap 35, it is disengaged from the feed pipe 34. The inactivating agent and animal cells are introduced into the interior of the reaction vessel 2 through the feed pipe 34. The first connecting rod 41 can be rotated by starting the drive motor 4. The first connecting rod 41 can drive the stirring shaft 42 to rotate. The stirring shaft 42 can cause the cells to combine with the virus. A filter plate 6 is slidably provided in the reaction vessel 2. A filter membrane is provided in the filter plate 6. An anti-clogging assembly 7 is provided in the reaction vessel 2. The anti-clogging assembly 7 is connected to the filter plate 6.

[0023] The anti-clogging component 7 includes a slide groove 71, which is formed on the inner surface of the reaction vessel 2. A spring 72 is fixedly installed on the inner surface of the slide groove 71, and a sliding plate 73 is fixedly installed on the upper end of the spring 72. The sliding plate 73 slides and fits against the inner surface of the slide groove 71. The sliding plate 73 is fixedly connected to the filter plate 6. A rectangular through groove 731 is formed inside the reaction vessel 2, and the rectangular through groove 731 is connected to the slide groove 71. A fixing plate 74 is fixedly installed on the inner surface of the rectangular through groove 731, and a first motor 75 is fixedly installed on the upper surface of the fixing plate 74. A second connecting rod 76 is provided at the output end of the first motor 75, and a half-face gear 77 is fixedly sleeved on the outer surface of the second connecting rod 76. A rectangular groove 78 is formed on the inner surface of the rectangular through groove 731, and a rectangular block 781 slides in the rectangular groove 78. The rectangular block 781 and the rectangular groove 78... The damped sliding connection includes a ring toothed plate 79 fixedly mounted on one side of the rectangular block 781, a half-face gear 77 meshing with the ring toothed plate 79, a top rod 791 fixedly mounted on the upper surface of the ring toothed plate 79, and the upper end of the top rod 791 in movable contact with the slide plate 73. A waterproof shell 741 is fixedly mounted on the upper surface of the fixed plate 74, and the second connecting rod 76 penetrates the waterproof shell 741 to improve waterproofness. Cell fluid is filtered through the filter membrane in the filter plate 6. To prevent the filter membrane from clogging, the second connecting rod 76 can be rotated by starting the first motor 75. The second connecting rod 76 drives the half-face gear 77 to rotate, and the half-face gear 77 drives the ring toothed plate 79 meshing with it to move back and forth. This allows the upper end of the top rod 791 to reciprocate in contact with the lower surface of the slide plate 73, causing the filter plate 6 to vibrate, which can prevent the filter membrane from clogging and thus improve filtration efficiency.

[0024] A fixing block 31 is fixedly provided on the lower surface of the can lid 3, and a sleeve 32 is fixedly provided on the outer surface of the can lid 3. The fixing block 31 is movably inserted into the sleeve 32. A first bolt 33 is threaded into the side of the sleeve 32. The first bolt 33 movably passes through the fixing block 31 to facilitate the removal of the can lid 3. A feed pipe 34 is connected to the can lid 3. A screw cap 35 is threadedly connected to the feed pipe 34. The screw cap 35 is connected to the feed pipe 34 through internal and external threads. Four springs 72 are provided, and the springs 72 are arrayed between the slide groove 71 and the slide plate 73. The rebound force can be improved by multiple springs 72. The cross-sectional shape of the rectangular groove 78 and the rectangular block 781 is "T" shaped to prevent the rectangular block 781 from detaching from the rectangular groove 78.

[0025] The disassembly and assembly assembly 5 includes a positioning block 51, the upper end of which is fixedly connected to the upper end of the stirring shaft 42. The lower end of the first connecting rod 41 is provided with a positioning groove 53. Both the positioning block 51 and the outer surface of the first connecting rod 41 are provided with through holes 52. A second bolt 54 is movably inserted into the through hole 52. A nut 55 is threaded onto the outer surface of the second bolt 54. When it is necessary to clean the stirring shaft 42, the tank cover 3 can be removed from the reaction tank 2. Then, the nut 55 can be rotated to remove it from the second bolt 54. The second bolt 54 can then be pulled out from the through hole 52, thereby disassembling the stirring shaft 42 for easy cleaning and subsequent production.

[0026] Working principle: When using this utility model, after rotating the cap 35, it is disengaged from the feed pipe 34. The inactivating agent and animal cells are introduced into the interior of the reaction vessel 2 through the feed pipe 34. By starting the drive motor 4, the first connecting rod 41 can be rotated, which can drive the stirring shaft 42 to rotate. The stirring shaft 42 can make the cells and viruses combine.

[0027] The cell fluid is filtered through the filter membrane in the filter plate 6. To prevent the filter membrane from clogging, the second connecting rod 76 can be rotated by starting the first motor 75. The second connecting rod 76 drives the half-face gear 77 to rotate. The half-face gear 77 drives the ring toothed plate 79 that meshes with it to move back and forth. This causes the upper end of the push rod 791 to reciprocate to contact the lower surface of the slide plate 73, thereby causing the filter plate 6 to vibrate. This can prevent the filter membrane from clogging and thus improve the filtration efficiency.

[0028] When it is necessary to clean the stirring shaft 42, the tank cover 3 can be removed from the reaction tank 2. Then, the nut 55 can be turned to remove it from the second bolt 54. After that, the second bolt 54 can be pulled out from the through hole 52, so that the stirring shaft 42 can be disassembled for cleaning and subsequent production.

[0029] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. An antigen purification and concentration apparatus comprising a base plate (1), characterised in that: The upper surface of the bottom plate (1) is fixedly provided with supports (11), reaction tanks (2) are fixedly arranged between the supports (11), tank covers (3) are arranged on the reaction tanks (2), driving motors (4) are fixedly arranged on the upper surfaces of the tank covers (3), first connecting rods (41) are arranged at the output ends of the driving motors (4), the first connecting rods (41) penetrate through the tank covers (3), stirring shafts (42) are connected to the first connecting rods (41), dismounting assemblies (5) are connected to the first connecting rods (41) and the stirring shafts (42), filter plates (6) are slidably arranged in the reaction tanks (2), and anti-blocking assemblies (7) are arranged in the reaction tanks (2) and connected with the filter plates (6). The anti-blocking assembly (7) comprises a sliding groove (71) which is formed in the inner surface of the reaction tank (2), a spring (72) which is fixedly arranged on the inner surface of the sliding groove (71), a sliding plate (73) which is fixedly arranged at the upper end of the spring (72) and slidably attached to the inner surface of the sliding groove (71), the sliding plate (73) is fixedly connected with the filter plate (6), a rectangular through groove (731) which is formed in the reaction tank (2) and communicates with the sliding groove (71), a fixed plate (74) which is fixedly arranged on the inner surface of the rectangular through groove (731), a first motor (75) which is fixedly arranged on the upper surface of the fixed plate (74), a second connecting rod (76) which is arranged at the output end of the first motor (75), a half-face gear (77) which is fixedly arranged on the outer side surface of the second connecting rod (76), a rectangular groove (78) which is formed in the inner surface of the rectangular through groove (731), a rectangular block (781) which is slidably arranged in the rectangular groove (78), an annular toothed plate (79) which is fixedly arranged on one side of the rectangular block (781), the half-face gear (77) is engaged with the annular toothed plate (79), and a jacking rod (791) which is fixedly arranged on the upper surface of the annular toothed plate (79) and movably contacts with the sliding plate (73).

2. An antigen purification and concentration apparatus as claimed in claim 1, wherein, The dismounting assembly (5) comprises a positioning block (51) which is fixedly connected to the upper end of the stirring shaft (42), a positioning groove (53) which is formed in the lower end of the first connecting rod (41), through holes (52) which are formed in the outer side surfaces of the first connecting rod (41) and the positioning block (51), and a second bolt (54) which is movably inserted into the through holes (52) and is threadedly sleeved with a nut (55).

3. An antigen purification and concentration apparatus as claimed in claim 1, wherein, A fixed block (31) is fixedly arranged on the lower surface of the tank cover (3), a sleeve (32) is fixedly arranged on the outer side surface of the tank cover (3), the fixed block (31) is movably inserted into the sleeve (32), a first bolt (33) is threadedly inserted into the side of the sleeve (32) and movably penetrates through the fixed block (31).

4. An antigen purification and concentration apparatus as claimed in claim 1, wherein, A feeding pipe (34) is connected to the tank cover (3), and a screw cap (35) is threadedly connected to the feeding pipe (34).

5. An antigen purification and concentration apparatus as defined in claim 1, wherein, The spring (72) is provided with four, and the spring (72) array distribution between the chute (71) and the slide plate (73).

6. An antigen purification and concentration apparatus as defined in claim 1, wherein, The cross section shape of the rectangular groove (78) and the rectangular block (781) are both "T" shape.

7. An antigen purification and concentration apparatus as defined in claim 1, wherein, The upper surface of the fixed plate (74) is fixedly provided with a waterproof shell (741), and the second connecting rod (76) penetrates the waterproof shell (741).

8. An antigen purification and concentration apparatus as defined in claim 1, wherein, The lower surface of the reaction tank (2) is provided with a discharge pipe (21), and the discharge pipe (21) is connected with an electric valve (22).

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