Exosome separating and filtering device

Abstract

The utility model discloses an exosome separating and filtering device which comprises a filtering tank, a discharging assembly and a collecting assembly are arranged at the two ends of the filtering tank respectively, the collecting assembly comprises a finished product tank, a vacuum pump is arranged on one side of the finished product tank, and the input end of the vacuum pump is communicated and connected with the top end of the finished product tank. When raw materials needing to be filtered are located in a raw material tank of the discharging assembly, air in a finished product tank is pumped out through a vacuum pump, negative pressure is formed in the raw material tank, at the moment, a valve on an output pipeline is opened, and the raw materials in the raw material tank can spontaneously flow into a filtering tank to be filtered; and the suction force generated by the negative pressure can promote the filtering rate, and when the filter screen in the filtering tank needs to be replaced, the filter screen can be disassembled only by opening the sealing shell, so that the maintenance efficiency of the device is improved.

Description

Technical Field

[0001] This utility model relates to the field of exosome technology, and in particular to an exosome separation and filtration device. Background Technology

[0002] Exosomes are tiny particles released by cells, ranging in size from tens to over a hundred nanometers, about one-thousandth the thickness of a human hair. They act as tiny messengers between cells, carrying proteins, genetic material, and other components to help cells transmit information or repair damaged tissue. For example, when a part of the body is injured, stem cells release exosomes; these particles deliver repair signals to surrounding cells, promoting healing. Current medical research is exploring the use of exosomes to deliver drugs or label diseases, providing new directions for treating cancer, nerve damage, and other conditions.

[0003] Patent document CN215310795U discloses an exosome separation device, which includes a centrifugation component, a filtration component, and a separation component. The centrifugation component includes a centrifuge tube and a motor. The bottom of the centrifuge tube is connected to the output shaft of the motor, and the top of the centrifuge tube is connected to the centrifuge tube cap via a connecting strip. The separated liquid after centrifugation by the centrifugation component enters the filtration component. The filtration component has multiple layers of filter screens arranged vertically, with a distance of 2-5 cm between adjacent layers. The separated liquid is transported to the separation component through the drain pipe of the filtration component. The separation component contains nutrient solution and biomagnetic beads. The biomagnetic beads adsorb and separate exosomes based on their properties. The centrifugation component first separates different cells in the separated liquid, and then the filter screen in the filtration component filters out large molecular cells. The biomagnetic beads can adsorb exosomes based on their properties, resulting in higher separation purity.

[0004] In the prior art of the aforementioned patent, the filter screens used for filtering exosomes are generally ultrafiltration screens with small pore sizes. Therefore, they need to be cleaned after a period of use, otherwise the screens will become clogged. However, when the aforementioned device filters exosomes, it relies solely on the gravity of the solution to filter within the filter tank, resulting in slow filtration efficiency. Furthermore, cleaning the multiple filter screens in the filter tank is inconvenient, requiring the disassembly of the pipes at both ends of the filter screens, opening of the filter tank, and removal of the filter screens for cleaning. Utility Model Content

[0005] Purpose of the utility model: The purpose of this utility model is to provide an exosome separation and filtration device to solve the above-mentioned shortcomings in the prior art.

[0006] Technical solution: An exosome separation and filtration device includes a filter tank, with a feeding component and a collection component respectively provided at both ends of the filter tank. The collection component includes a finished product tank, and a vacuum pump is provided on one side of the finished product tank. The input end of the vacuum pump is connected to the top of the finished product tank. An output pipe is connected between the finished product tank and the filter tank. A valve is provided on the output pipe. A through groove is opened at the top of the filter tank. A sealing shell is provided on the outside of the filter tank. A first filter screen, a second filter screen, and a third filter screen are provided inside the filter tank.

[0007] As a further description of the above technical solution: the filter tank is provided with two threaded parts, which are respectively located on both sides of the through groove, and the inner walls of both ends of the sealing shell are respectively threadedly connected to the two threaded parts.

[0008] As a further description of the above technical solution: the top ends of the first filter screen, the second filter screen and the third filter screen are all fixedly installed with mounting rings, and the two ends of the mounting rings are all fixedly installed with plug-in parts. The top end of the filter tank is provided with multiple plug-in slots, and the multiple plug-in parts are adapted to each other with the plug-in slots. The mounting rings are tightly attached to the inner wall of the sealing shell.

[0009] As a further description of the above technical solution: a viewing glass is provided at the top of the sealing shell.

[0010] As a further description of the above technical solution: the feeding assembly includes a raw material tank, and an input pipe is installed between the raw material tank and the filter tank.

[0011] As a further description of the above technical solution: an injection pipe is connected to one side of the raw material tank, and a sealing plug is threadedly connected to the top end of the injection pipe.

[0012] As a further description of the above technical solution: the output end of the vacuum pump is connected to an exhaust pipe, and the other end of the exhaust pipe is connected to the top of the raw material tank.

[0013] As a further description of the above technical solution: a fourth filter screen is provided at the top of the finished product tank, and the fourth filter screen is located at the air extraction pipe.

[0014] Beneficial effects: When the raw material to be filtered is in the raw material tank of the feeding component, the air inside the finished product tank is extracted by the vacuum pump, creating a negative pressure inside the raw material tank. At this time, the valve on the output pipe is opened, and the raw material in the raw material tank can flow into the filter tank spontaneously for filtration. Through three layers of filter screens with different pore sizes, the exosomes are filtered and finally enter the finished product tank. The suction generated by the negative pressure can promote the filtration rate. When the filter screen in the filter tank needs to be replaced, the filter screen can be disassembled simply by opening the sealing shell, which increases the maintenance efficiency of the device. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall three-dimensional structure of an exosome separation and filtration device proposed in this utility model;

[0016] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0017] Figure 3 This utility model Figure 2 A magnified structural diagram at point A;

[0018] Figure 4 This is a three-dimensional exploded view of the filter tank of this utility model;

[0019] Figure 5 This is a schematic diagram of the main structure of this utility model;

[0020] Figure 6 A cross-sectional structural diagram showing the top of the sealing shell of this utility model with a visual glass panel.

[0021] Legend:

[0022] 1. Raw material tank; 2. Injection pipe; 3. Sealing plug; 4. Input pipe; 5. Output pipe; 6. Sealing shell; 7. Valve; 8. Finished product tank; 9. Vacuum pump; 10. Evacuation pipe; 11. Exhaust pipe; 12. Filter tank; 13. Threaded part; 14. Through groove; 15. Mounting ring; 16. First filter screen; 17. Second filter screen; 18. Third filter screen; 19. Insertion part; 20. Insertion groove; 21. Fourth filter screen; 22. Visual glass. Detailed Implementation

[0023] To make the technical solution of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0024] Reference Figure 1-6An exosome separation and filtration device includes a filter tank 12. A feeding assembly and a collection assembly are respectively provided at both ends of the filter tank 12. The collection assembly includes a finished product tank 8. A vacuum pump 9 is provided on one side of the finished product tank 8, and the input end of the vacuum pump 9 is connected to the top of the finished product tank 8. An output pipe 5 connects the finished product tank 8 and the filter tank 12. A valve 7 is provided on the output pipe 5. A through groove 14 is opened at the top of the filter tank 12. A sealing shell 6 is fitted around the outside of the filter tank 12. A first filter screen 16, a second filter screen 17, and a third filter screen 18 are provided inside the filter tank 12. The raw material to be filtered is located in the feeding assembly. In the raw material tank 1, when the air inside the finished product tank 8 is extracted by the vacuum pump 9, creating a negative pressure inside the raw material tank 1, the valve 7 on the output pipe 5 is opened, and the raw material in the raw material tank 1 can spontaneously flow into the filter tank 12 for filtration. Through three layers of filter screens with different pore sizes, the exosomes are filtered and finally enter the finished product tank 8. The suction generated by the negative pressure can promote the filtration rate. When the filter screen in the filter tank 12 needs to be replaced, the filter screen can be disassembled simply by opening the sealing shell 6, which increases the maintenance efficiency of the device. In addition, the bottom of the finished product tank 8 is provided with a discharge port to collect the filtered exosomes.

[0025] As a preferred technical solution of this embodiment, the filter tank 12 is provided with two threaded portions 13, which are respectively provided on both sides of the through groove 14. The inner walls of both ends of the sealing shell 6 are respectively threadedly connected to the two threaded portions 13. The sealing shell 6 is connected to the filter tank 12 body through the threaded portions 13. When it is necessary to disassemble the sealing shell 6, it is only necessary to rotate the sealing shell 6 to disassemble it. The disassembled sealing shell 6 can be temporarily attached to the output pipe 5 to prevent loss.

[0026] As a preferred embodiment, the top ends of the first filter screen 16, the second filter screen 17, and the third filter screen 18 are all fixedly equipped with mounting rings 15. Both ends of the mounting rings 15 are fixedly equipped with insertion parts 19. The top end of the filter tank 12 is provided with multiple insertion slots 20. The multiple insertion parts 19 are adapted to the insertion slots 20. The mounting rings 15 are tightly attached to the inner wall of the sealing shell 6. The mounting rings 15 can seal the gaps in the through grooves 14, so that the mounting rings 15 are tightly attached to the inner wall of the sealing shell 6. The insertion parts 19 can limit the multiple filters and prevent relative sliding between the filters.

[0027] As a preferred technical solution in this embodiment, the top of the sealing shell 6 is provided with a visualization glass 22; the visualization glass 22 can observe the condition of multiple filters inside the filter tank 12.

[0028] As a preferred technical solution of this embodiment, the feeding component includes a raw material tank 1, and an input pipe 4 is installed between the raw material tank 1 and the filter tank 12; the raw material in the raw material tank 1 can enter the filter tank 12 for filtration through the input pipe 4.

[0029] As a preferred technical solution of this embodiment, an injection pipe 2 is connected to one side of the raw material tank 1, and a sealing plug 3 is threadedly connected to the top end of the injection pipe 2; raw materials can be injected into the raw material tank 1 through the injection pipe 2, and the sealing plug 3 can seal the raw material tank 1.

[0030] As a preferred technical solution of this embodiment, the output end of the vacuum pump 9 is connected to an exhaust pipe 11, and the other end of the exhaust pipe 11 is connected to the top of the raw material tank 1. The gas drawn out by the vacuum pump 9 from the finished product tank 8 can be discharged into the raw material tank 1 through the exhaust pipe 11, increasing the gas pressure inside the raw material tank 1, so that the raw material in the raw material tank 1 can be forced into the filter tank 12 during the filtration process.

[0031] As a preferred technical solution in this embodiment, a fourth filter screen 21 is provided at the top of the finished product tank 8, and the fourth filter screen 21 is located at the suction pipe 10; the pore size of the fourth filter screen 21 is smaller than the pore size of the exosomes, and the fourth filter screen 21 is provided to prevent the vacuum pump 9 from extracting the exosomes during the suction process.

[0032] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. An exosome isolation filtration device comprising a filtration canister (12), characterized in that, The filter tank (12) is provided with a discharging assembly and a collecting assembly at two ends respectively, the collecting assembly comprises a finished product tank (8), one side of the finished product tank (8) is provided with a vacuum pump (9), the input end of the vacuum pump (9) is in communication connection with the top end of the finished product tank (8), an output pipe (5) is in communication connection between the finished product tank (8) and the filter tank (12), the output pipe (5) is provided with a valve (7), a through groove (14) is formed in the top end of the filter tank (12), the filter tank (12) is sleeved with a sealing shell (6) outside, and the inside of the filter tank (12) is provided with a first filter screen (16), a second filter screen (17) and a third filter screen (18).

2. The exosome isolation filter device of claim 1, wherein, Two threaded parts (13) are arranged on the filter tank (12), and the two threaded parts (13) are arranged on the two sides of the through groove (14), respectively, and the inner walls of the two ends of the sealing shell (6) are in threaded connection with the two threaded parts (13), respectively.

3. The exosome isolation filter device of claim 1, wherein, The top end of the first filter screen (16), the second filter screen (17) and the third filter screen (18) is fixedly provided with a mounting ring (15), the two ends of the mounting ring (15) are fixedly provided with a plug-in part (19), the top end of the filter tank (12) is provided with a plurality of plug-in grooves (20), and the plug-in parts (19) and the plug-in grooves (20) are matched with each other, and the mounting ring (15) is tightly attached to the inner wall of the sealing shell (6).

4. The exosome isolation filter device of claim 1, wherein, The top end of the sealing shell (6) is provided with a visual glass (22).

5. The exosome isolation filter device of claim 1, wherein, The discharging assembly comprises a raw material tank (1), and an input pipe (4) is in communication connection between the raw material tank (1) and the filter tank (12).

6. The exosome isolation filtration device of claim 5, wherein, One side of the raw material tank (1) is in communication connection with an injection pipe (2), and the top end of the injection pipe (2) is in threaded sealing connection with a sealing plug (3).

7. The exosome isolation filter device of claim 1, wherein, The output end of the vacuum pump (9) is in communication connection with an exhaust pipe (11), and the other end of the exhaust pipe (11) is in communication connection with the top end of the raw material tank (1).

8. The exosome isolation filter device of claim 1, wherein, The top end of the finished product tank (8) is provided with a fourth filter screen (21), and the fourth filter screen (21) is arranged at the air exhaust pipe (10). The top end of the finished product tank (8) is provided with a fourth filter screen (21), and the fourth filter screen (21) is arranged at the air exhaust pipe (10).

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