Rapid pine wood nematode separation device

Abstract

The utility model relates to a pine wood nematode rapid separation device, and relates to the technical field of pine wood nematode separation, the pine wood nematode rapid separation device comprises a separator and a filter, the separator is used for separating nematodes, the filter is used for filtering a solution obtained after nematode separation, the separator comprises an outer cylinder, a centrifugal disc, a pressing block and a centrifugal barrel, an opening is formed in the upper end of the outer barrel, the centrifugal disc is installed at the upper end of the outer barrel, a centrifugal motor is installed in the centrifugal disc, the centrifugal barrel is installed in the outer barrel, centrifugal holes are formed in the centrifugal barrel, an output shaft of the centrifugal motor is in driving connection with the pressing block, and the pressing block is installed at the upper end of the centrifugal barrel. The centrifugal barrel is driven by the pressing block to rotate, and a water through hole is formed in the bottom of the outer barrel. The device has the effect of further improving the pine wood nematode separation efficiency.

Description

Technical Field

[0001] This application relates to the field of pine wilt disease separation technology, and in particular to a rapid pine wilt disease separation device. Background Technology

[0002] Pine wilt disease is a serious threat to global forest ecosystems, primarily transmitted by the pine wilt nematode. With the rapid development of global trade and logistics, the spread of this disease has been continuously expanding, causing enormous damage to forest resources and the ecological environment. Therefore, the rapid and efficient isolation of pine wilt nematodes has become a crucial link in disease monitoring and control, which is of great significance for protecting forest resources.

[0003] Traditional methods for isolating pine wood nematodes include the Bellman funnel method, the shallow dish method, and the negative pressure separation method. The Bellman funnel method and the shallow dish method both require 24 hours of soaking to obtain the soaking solution, followed by microscopic observation for the presence of pine wood nematodes. These methods require the wood to be split into pieces as small as matchsticks and have a long separation time, making them unsuitable for rapid detection. The negative pressure separation method utilizes negative pressure technology; when water flows through the microtubes inside the sample, it carries the nematodes out, resulting in a clean nematode solution sample. This method is efficient and fast, but it has limitations, requiring the wood to be planar and limiting the sample size.

[0004] Therefore, existing centrifugal separation devices generally suffer from low separation efficiency and cumbersome operation, especially when processing large numbers of samples, making it difficult to meet the demand for rapid and efficient separation. Utility Model Content

[0005] To further improve the separation efficiency of pine wood nematodes, this application provides a rapid separation device for pine wood nematodes.

[0006] The rapid separation device for pine wood nematodes provided in this application adopts the following technical solution:

[0007] A rapid pine wood nematode separation device includes a separator and a filter. The separator is used to separate the nematodes, and the filter is used to filter the solution after nematode separation. The separator includes an outer cylinder, a centrifuge disc, a pressure block, and a centrifuge barrel. The upper end of the outer cylinder is open. The centrifuge disc is installed on the upper end of the outer cylinder and a centrifuge motor is installed inside the centrifuge disc. The centrifuge barrel is installed inside the outer cylinder and has centrifugation holes. The output shaft of the centrifuge motor is driven and connected to the pressure block, which is installed on the upper end of the centrifuge barrel and drives the centrifuge barrel to rotate. A water passage hole is provided at the bottom of the outer cylinder.

[0008] By employing the above technical solution, rapid separation of pine wood nematodes was achieved. The separator uses a centrifugal motor within the centrifuge disc to drive the pressure block to rotate, thereby causing the centrifuge drum to rotate at high speed. Centrifugal force is used to separate the nematodes from the wood sample. The open design at the top of the outer cylinder facilitates sample placement and removal, while the centrifuge holes on the drum ensure smooth liquid drainage during separation, preventing nematodes from being lost with the liquid. A filter further filters the separated solution, effectively improving the purity and efficiency of nematode separation.

[0009] In one specific implementation, a limiting block is installed on the lower end face of the inner cavity of the outer cylinder, and the limiting block can rotate relative to the outer cylinder. A limiting rod is correspondingly installed on the lower end face of the centrifuge barrel, and the limiting rod can be inserted into the limiting block. The centrifuge barrel has an opening at the upper end, and a limiting tooth is provided on the circumferential part of the opening at the upper end of the centrifuge barrel. A driving tooth is correspondingly provided on the pressure block, and the driving tooth can mesh with the limiting tooth. The output shaft of the centrifuge motor can be inserted into and driven by the upper end face of the pressure block.

[0010] By adopting the above technical solution, the cooperation between the limiting block and the limiting rod can effectively prevent the centrifuge tank from shifting during high-speed rotation, ensuring the stability of the centrifugation process. The limiting teeth at the upper opening of the centrifuge tank mesh with the driving teeth on the pressure block, realizing precise driving of the centrifuge tank by the pressure block and improving transmission efficiency. The plug-in drive connection between the centrifuge motor output shaft and the upper end face of the pressure block makes disassembly and assembly more convenient, facilitating equipment maintenance and cleaning.

[0011] In one specific implementation, the centrifuge barrel is provided with multiple centrifugation holes, which are distributed on the side wall of the centrifuge barrel.

[0012] By adopting the above technical solution, multiple centrifugation holes are opened on the centrifuge tank and distributed on the side wall of the centrifuge tank. This can effectively increase the liquid discharge efficiency during centrifugation, thereby improving the separation speed and effect of pine wood nematodes. The distributed centrifugation holes can ensure that the liquid is discharged evenly under the action of centrifugal force, reduce liquid residue, and avoid the problem of incomplete separation caused by uneven pressure in the centrifuge tank.

[0013] In one specific implementation, the pressure block includes a limiting ring, connecting rods, and a connecting block. Several connecting rods are provided and are evenly installed on the circumferential part of the limiting ring. The connecting block is installed on one end of the connecting rods away from the limiting ring and on the central part of the limiting ring. The limiting ring is connected to the output shaft of the centrifugal motor through the connecting block.

[0014] By adopting the above technical solution, the design of the limiting ring, connecting rods, and connecting block makes the pressure block structure more stable and effectively transmits the power of the centrifugal motor. Several connecting rods are evenly distributed around the circumference of the limiting ring, ensuring the balance of the pressure block during high-speed rotation and preventing vibration or damage caused by uneven force. The connecting block is located at the center of the limiting ring and is plugged into the output shaft of the centrifugal motor for drive connection, achieving a reliable connection between the pressure block and the centrifugal motor and ensuring the stability and efficiency of power transmission.

[0015] In one specific implementation, a waterproof touchscreen is installed on the centrifuge tray, the waterproof touchscreen being electrically connected to the centrifuge motor, and the waterproof touchscreen being used to adjust the centrifugation time according to different types of wood.

[0016] By adopting the above technical solution, a waterproof touchscreen is installed on the centrifuge disc, enabling electrical connection with the centrifuge motor and allowing adjustment of centrifugation time according to the characteristics of different types of wood. This design makes the separation device more flexible and adaptable, effectively improving the efficiency and accuracy of pine wilt disease separation, while simplifying the operation process and reducing errors caused by human intervention.

[0017] In one specific implementation, the filter includes a filter housing and filter components, wherein a plurality of filter components are provided, and the plurality of filter components are all installed in the filter housing.

[0018] By adopting the above technical solution, the filter box provides installation space for the filter components, which facilitates centralized management and operation; by setting up multiple filter components, components with different filtration precisions can be selected as needed, thereby effectively removing impurities in the solution and ensuring the purity of pine wood nematodes.

[0019] In one specific implementation, the filter assembly includes a filter cup, a support tube, and an inner liner. The filter cup is installed at the upper end of the support tube and is connected to a water outlet pipe. The inner liner is installed inside the support tube. A water outlet pipe is installed on the side wall of the support tube at the lower end of the inner liner. A water blowing pipe extends through the side wall of the filter housing and a drain valve is installed on the water outlet pipe.

[0020] By adopting the above technical solution, the filtration assembly consists of a filter cup, a support tube, and an inner liner, achieving multi-stage filtration and collection of the separated solution. The filter cup is installed at the upper end of the support tube and connected to the outlet pipe, ensuring that the solution can flow smoothly and undergo filtration. The inner liner is installed inside the support tube, further improving the filtration accuracy. A drain valve is installed on the outlet pipe to easily control the discharge of the filtrate. A blower pipe extends through the side wall of the filter box, providing convenient conditions for subsequent cleaning or auxiliary operations. Among these features, the design of connecting the filter cup to the outlet pipe optimizes the liquid flow path, the addition of the inner liner enhances the filtration effect, the drain valve improves operational convenience, and the layout of the blower pipe expands the functionality of the device.

[0021] In one specific implementation, a base is also included, with the lower end of the support tube supported on the base.

[0022] By adopting the above technical solution, the base provides stable support for the support tube, preventing it from tilting or becoming unstable due to external vibration or liquid impact during filtration, thereby improving the reliability of the entire device. Simultaneously, the base also increases the overall stability of the device, preventing tipping due to an unstable center of gravity and ensuring the safety and continuity of the separation and filtration process.

[0023] In one specific implementation scheme, the quick-release structure includes a positioning pin and a positioning spring. An insertion block is provided on the inner wall of the filter cup, and an insertion groove is provided on the outer wall of the outer cylinder corresponding to the insertion block. The insertion block can slide within the insertion groove. An installation groove is provided on the inner wall of the insertion groove. The positioning spring is installed in the installation groove, and the positioning pin is slidably connected within the installation groove. The limiting spring is always in a compressed state, and the positioning pin can slide out of the installation groove under the force of the limiting spring. The end of the positioning pin facing away from the installation groove is spherical. One end of the positioning spring is fixedly connected to the positioning pin, and the other end of the positioning spring is connected to the inner wall of the installation groove. A limiting groove is provided on the insertion block, and the inner wall of the limiting groove is chamfered corresponding to the positioning pin.

[0024] By adopting the above technical solution, the quick-release structure makes the installation and disassembly of the separator and filter more convenient. The cooperative design of the positioning pin and positioning spring allows for quick locking during the insertion of the connector block into the connector slot, achieved through the automatic sliding of the positioning pin under the force of the limiting spring. Simultaneously, the spherical design reduces frictional resistance during insertion. For disassembly, simply apply external force to retract the positioning pin into the mounting slot, and the connector block can be easily removed, thereby improving equipment maintenance and cleaning efficiency. This design effectively solves the problem of inconvenient connection between the separator and filter in traditional devices, improving the overall ease of operation.

[0025] In summary, this application includes at least one of the following beneficial technical effects:

[0026] 1. By using the centrifuge disc and centrifuge barrel in the separator, the centrifuge motor drives the pressure block to rotate the centrifuge barrel at high speed, generating centrifugal force to efficiently separate pine wood nematodes from wood samples, significantly improving the separation efficiency;

[0027] 2. The multi-stage filtration components in the filter can effectively filter the separated solution, ensuring that nematodes are fully separated from impurities, thus improving the purity and accuracy of the separation;

[0028] 3. The waterproof touchscreen allows for adjustment of centrifugation time based on the characteristics of different types of wood, making it easy to operate and highly adaptable, further simplifying the separation process and meeting diverse needs. Attached Figure Description

[0029] Figure 1 This is a structural schematic diagram of an embodiment of this application.

[0030] Figure 2 This is a front view of an embodiment of this application.

[0031] Figure 3 for Figure 2 A cross-sectional view along the AA direction.

[0032] Figure 4 for Figure 3 A magnified view of part A in the middle.

[0033] Explanation of reference numerals in the attached drawings: 1. Separator; 2. Filter; 3. Outer cylinder; 31. Insertion groove; 4. Centrifuge disc; 5. Pressure block; 51. Limiting ring; 52. Connecting rod; 53. Connecting block; 6. Centrifuge tank; 61. First tank body; 62. Second tank body; 63. Third tank body; 71. Filter cup; 711. Insertion block; 72. Support tube; 73. Inner liner; 74. Base; 75. Water outlet pipe; 8. Quick release structure; 81. Positioning pin; 82. Positioning spring. Detailed Implementation

[0034] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two elements or the interaction between two elements. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0035] This application discloses a rapid separation device for pine wood nematodes.

[0036] like Figure 1 and Figure 2As shown, the rapid pine wood nematode separation device includes a separator 1 and a filter 2. The separator 1 is used to separate the nematodes from the wood sample, and the filter 2 is used to further filter the separated solution. The separator 1 is installed on the upper end of the filter 2 via a quick-release structure 8, thereby achieving efficient pine wood nematode separation. Specifically, the separator 1 includes an outer cylinder 3, a centrifuge disc 4, a pressure block 5, and a centrifuge barrel 6. The upper end of the outer cylinder 3 is open, and the centrifuge disc 4 is installed on the upper end of the outer cylinder 3, with a centrifuge motor installed inside. The centrifuge barrel 6 is installed inside the outer cylinder 3, and its side wall has multiple centrifuge holes for the discharge of liquid during the separation process. The multiple centrifuge holes are distributed on the outer circumferential outer wall of the centrifuge barrel. The output shaft of the centrifuge motor is driven and connected to the pressure block 5, which is installed on the upper end of the centrifuge barrel 6. The pressure block 5 drives the centrifuge barrel 6 to rotate at high speed. The bottom of the outer cylinder 3 is provided with a water passage hole for the filtered liquid to flow into the filter 2.

[0037] The centrifuge tank 6 includes a first tank body 61, a second tank body 62, and a third tank body 63. A pressing block 5 is installed on the first tank body. A first locking block is circumferentially arranged on the outer wall of the upper end of the first tank body 61. A first locking groove is correspondingly arranged on the upper end of the second tank body 62, and the first locking block is engaged in the first locking groove. A second locking block is circumferentially arranged on the outer wall of the upper end of the second tank body 62. A second locking groove is correspondingly arranged on the upper end of the third tank body 63, and the second locking block is engaged in the second locking groove. The third tank body is rotatably connected to the outer cylinder 3. Centrifuge holes are provided on the side walls of the first tank body 61, the second tank body 62, and the third tank body 63. The size of the centrifuge holes gradually decreases from the inside to the outside, and is set to 500, 300, and 100 mesh, respectively.

[0038] The outer cylinder 3 can be made of stainless steel, which has high strength and corrosion resistance. The centrifuge disc 4 has a circular structure, and its upper surface is equipped with a waterproof touch screen for adjusting the centrifugation time according to different types of wood. The centrifuge motor can be an AC servo motor, which features high-precision control and stable operation. The centrifuge tank 6 has a cylindrical structure, and the centrifuge holes on its side wall can be designed as circular or elliptical as needed, with a diameter ranging from 1 mm to 3 mm. The distribution of the centrifuge holes must be uniform to ensure that the liquid can be discharged evenly during the separation process.

[0039] The pressure block 5 consists of a limiting ring 51, a connecting rod 52, and a connecting block 53. The limiting ring 51 is a circular ring structure made of high-strength material. Several connecting rods 52 are evenly installed on the circumferential part of the limiting ring 51. One end of the connecting rod 52 is installed on the limiting ring 51, and the other end of the connecting rod 52 extends toward the axial part of the limiting ring 51. The connecting block 53 is installed on one end of the several connecting rods 52 away from the limiting ring 51, thereby installing the connecting block 53 at the axial part of the limiting ring 51.

[0040] A limit block is installed on the lower inner surface of the outer cylinder 3. The limit block has a circular structure and can rotate relative to the outer cylinder 3. A limit rod is correspondingly installed on the lower end surface of the centrifuge barrel 6. The limit rod can be inserted into the limit block to play a positioning role. Limit teeth are opened on the circumferential part of the upper opening of the centrifuge barrel 6. A corresponding drive tooth is provided on the pressure block 5. The drive tooth and the limit tooth are meshed with each other, thereby realizing the effective driving of the centrifuge barrel 6 by the pressure block 5.

[0041] like Figure 3 As shown in the embodiment of this application, the filter 2 includes a filter cup 71, a support tube 72, an inner liner 73, and a base 74. The lower end of the support tube 72 is mounted on the base 74 and is sealed to the base 74. The filter cup 71 is mounted on the upper end of the support tube 72 and is connected to the support tube 72. The diameter of the support tube 72 is smaller than the diameter of the lower end of the filter cup 71. The filter cup 71 can be configured as a cylindrical cup or a funnel-shaped cup as needed. The inner liner 73 is installed inside the support tube 72. The inner liner 73 has a cylindrical structure and can be made of nylon or polyethylene, which has good wear resistance and filtration performance. A water outlet pipe 75 is installed on the side wall of the support tube 72 at the lower end of the inner liner 73. The water outlet pipe 75 extends out of the side wall of the filter box and is equipped with a drain valve. The drain valve can be a ball valve commonly used in the market. The flow rate can be controlled by rotating the ball.

[0042] like Figure 4As shown in this embodiment, the quick-release structure 8 includes a positioning pin 81 and a positioning spring 82. Multiple sets of insertion blocks 711 are circumferentially arranged on the upper end of the inner wall of the filter plate. The insertion blocks 711 are preferably rectangular blocks and are arranged along the height direction of the filter cup 71. An insertion groove 31 is provided on the circumferential part of the outer wall of the outer cylinder 3 corresponding to the insertion blocks 711. The insertion blocks 711 can be inserted into the insertion groove 31. An installation groove is provided on the inner wall of the insertion groove 31. The positioning spring 82 is installed in the installation groove, and the positioning pin 81 is slidably connected in the installation groove. One end of the positioning spring 82 is fixedly connected to the inner wall of the installation groove, and the other end of the positioning spring 82 is fixedly connected to the positioning pin 81. The end of the positioning pin 81 facing away from the positioning spring 82 is spherical. A limiting groove is provided on the insertion block 711 corresponding to the positioning pin 81. The inner side of the limiting groove... With the chamfered wall setting, when the separator 1 and filter 2 need to be connected, the outer cylinder 3 is inserted into the filter cup 71, and the insertion block 711 is inserted into the insertion groove 31. The upper end of the insertion block 711 supports the outer cylinder 3. When the insertion block 711 is inserted into the insertion groove 31, the side wall of the insertion block 711 presses against the positioning pin 81. The positioning spring 82 contracts and stores the force. When the positioning pin 81 is facing the limiting groove, the positioning spring 82 releases the force and pushes the positioning pin 81 to engage in the limiting groove. At the same time, the inner side wall of the limiting groove is chamfered. When the separator 1 and filter 2 need to be separated, an upward force is applied to the separator 1. The positioning pin 81 can be pressed by the inner side wall of the limiting groove and the side wall of the insertion block 711 and gradually retracted into the installation groove until the separator 1 and filter 2 are completely separated.

[0043] The implementation principle of the rapid pine wood nematode separation device in this embodiment is as follows: the centrifugal motor inside the centrifuge disc 4 drives the pressure block 5 to rotate, which in turn drives the centrifuge tank 6 to rotate at high speed, generating a strong centrifugal force. The wood sample is subjected to centrifugal force within the centrifuge tank 6, and the nematodes are gradually separated and discharged through centrifuge holes on the side wall of the tank 6. Compared with traditional manual separation methods and funnel methods, the separation efficiency of this embodiment is significantly improved, and the operation is simpler, effectively meeting the needs of rapid detection.

[0044] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A pine wood nematode rapid separation device comprising a separator (1) for separating nematodes and a filter (2) for filtering the solution after the separation of nematodes, the separator (1) being installed on the upper end of the filter (2) by a quick release structure (8), characterized in that: The separator (1) comprises an outer cylinder (3), a centrifugal disc (4), a pressing block (5) and a centrifugal barrel (6), the outer cylinder (3) is provided with an open upper end, the centrifugal disc (4) is installed on the upper end of the outer cylinder (3), a centrifugal motor is installed in the centrifugal disc (4), the centrifugal barrel (6) is installed in the outer cylinder (3), the centrifugal barrel (6) is provided with a centrifugal hole on the upper end, the output shaft of the centrifugal motor is drivingly connected with the pressing block (5), the pressing block (5) is installed on the upper end of the centrifugal barrel (6), the centrifugal barrel (6) is driven to rotate by the pressing block (5), and the outer cylinder (3) is provided with a water hole at the bottom.

2. The rapid isolation device for pine wood nematode according to claim 1, characterized in that: A limiting block is installed on the inner lower end face of the outer cylinder (3), the limiting block can rotate relative to the outer cylinder (3), a limiting rod is installed on the lower end face of the centrifugal barrel (6), the limiting rod can be inserted into the limiting block, the centrifugal barrel (6) is provided with an open upper end, limiting teeth are arranged on the circumferential part of the open upper end of the centrifugal barrel (6), driving teeth are arranged on the pressing block (5), the driving teeth can be meshed with the limiting teeth, and the output shaft of the centrifugal motor can be drivingly connected with the upper end face of the pressing block (5).

3. The rapid isolation device for pine wood nematode according to claim 2, characterized in that: A plurality of centrifugal holes are arranged on the centrifugal barrel (6), and the plurality of centrifugal holes are dispersedly arranged on the side wall of the centrifugal barrel (6).

4. The rapid isolation device for pine wood nematode according to claim 2, characterized in that: The pressing block (5) comprises a limiting ring (51), a connecting rod (52) and a connecting block (53), a plurality of connecting rods (52) are arranged, the plurality of connecting rods (52) are uniformly installed on the circumferential part of the limiting ring (51), the connecting block (53) is installed on one end of the plurality of connecting rods (52) away from the limiting ring (51), and the connecting block (53) is installed on the axial center part of the limiting ring (51). The limiting ring (51) is drivingly connected with the output shaft of the centrifugal motor through the connecting block (53).

5. The rapid isolation apparatus for pine wood nematode according to claim 1, characterized in that: A waterproof touch screen is installed on the centrifugal disc (4), the waterproof touch screen is electrically connected with the centrifugal motor, and the waterproof touch screen is used for adjusting the centrifugal time according to different wood.

6. The rapid isolation apparatus for pine wood nematode according to claim 1, characterized in that: The filter (2) comprises a filter box body and a plurality of filter assemblies, the plurality of filter assemblies are installed in the filter box body, the filter (2) comprises a filter cup (71), a supporting pipe (72) and an inner lining cylinder (73), the filter cup (71) is installed on the upper end of the supporting pipe (72), the filter cup (71) is communicated with a water outlet pipe (75), the inner lining cylinder (73) is installed in the supporting pipe (72), a water outlet pipe (75) is installed on the side wall of the supporting pipe (72) at the lower end of the inner lining cylinder (73), the water outlet pipe (75) penetrates through the side wall of the filter box body, and a water drain valve is installed on the water outlet pipe (75).

7. The rapid isolation device for pine wood nematode according to claim 6, characterized in that: A base (74) is further arranged, and the lower end of the supporting pipe (72) is supported on the base (74).

8. The rapid isolation device for pine wood nematode according to claim 6, characterized in that: The quick release structure (8) includes a positioning pin (81) and a positioning spring (82), an insertion block (711) is arranged on the inner side wall of the filter cup (71), an insertion groove (31) corresponding to the insertion block (711) is formed on the outer side wall of the outer cylinder (3), the insertion block (711) can slide in the insertion groove (31), a mounting groove is formed on the inner side wall of the insertion groove (31), the positioning spring (82) is mounted in the mounting groove, the positioning pin (81) is slidingly connected in the mounting groove, the limiting spring is always in a compressed state, the positioning pin (81) can slide out of the mounting groove under the action force of the limiting spring, and the end, away from the mounting groove, of the positioning pin (81) is provided as a spherical surface, one end of the positioning spring (82) is fixedly connected with the positioning pin (81), and the other end of the positioning spring (82) is connected with the inner side wall of the mounting groove, a limiting groove is formed on the insertion block (711), and the inner side wall of the limiting groove is provided with a chamfer corresponding to the positioning pin (81).

Images