Rapid centrifugal separation device for nucleic acid extraction

Abstract

The utility model discloses a rapid centrifugal separation device for nucleic acid extraction and relates to the technical field of nucleic acid extraction and separation equipment. The clamping device comprises a machine body and a driving assembly, a limiting disc is fixedly arranged at the top end of a bottom plate, positioning assemblies distributed in an array mode are fixedly arranged at the top end of the driving assembly, each positioning assembly comprises an external sliding way, the external sliding ways are fixedly installed at the top end of the bottom plate, clamping blocks distributed symmetrically are movably inserted into the external sliding ways, and a threaded rod is rotationally arranged at the top end of the bottom plate; the outer side of the threaded rod is sleeved with three threaded sliding blocks in a threaded mode, connecting plates are fixedly arranged at the two ends of each threaded sliding block respectively, and inclined plates which are symmetrically distributed are fixedly arranged on the outer sides of the connecting plates. According to the centrifugal tube clamping and positioning device, the machine body, the external sliding way, the clamping block, the threaded rod, the threaded sliding block, the connecting plate, the inclined plate and other structures are arranged to be matched with one another, centrifugal tubes of different specifications can be clamped and positioned, and therefore centrifugal separation can be conveniently conducted on the centrifugal tubes of different specifications, and the use range of the device can be widened.

Description

Technical Field

[0001] This utility model relates to the technical field of nucleic acid extraction and separation equipment, specifically a rapid centrifugal separation device for nucleic acid extraction. Background Technology

[0002] Nucleic acid extraction is the process of isolating DNA or RNA from biological samples, commonly used in molecular biology experiments such as gene analysis, cloning, and PCR. Centrifugation in nucleic acid extraction primarily involves separating nucleic acids released after cell lysis from cells, proteins, and other impurities through high-speed centrifugation. During this process, centrifugal force causes nucleic acids to precipitate to the bottom of the centrifuge tube, while the supernatant contains impurities. Centrifugation conditions (such as speed and time) can be flexibly adjusted to optimize nucleic acid purity and recovery rate, thus providing high-quality nucleic acid samples for subsequent experiments.

[0003] However, existing equipment is not convenient for adjusting, clamping, and positioning centrifuge tubes of different sizes, which makes the separation device relatively simple in terms of practicality, limited in its application range, and has poor centrifugation efficiency. In order to address the above problems, the inventors proposed a rapid centrifugation separation device for nucleic acid extraction. Utility Model Content

[0004] To address the problem of not being able to clamp and position centrifuge tubes of different sizes, the purpose of this invention is to provide a rapid centrifugation and separation device for nucleic acid extraction.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a rapid centrifugation separation device for nucleic acid extraction, comprising a body and a drive assembly, wherein the drive assembly is installed in the body, a base plate is connected to the outside of the drive assembly, a limiting plate is fixedly provided at the top of the base plate, and a positioning assembly arranged in an array is fixedly provided at the top of the drive assembly, the positioning assembly comprising an external slide rail, the external slide rail being fixedly installed at the top of the base plate, and symmetrically distributed clamping blocks being movably inserted in the external slide rail, a threaded rod being rotatably provided at the top of the base plate, three threaded sliders being threadedly sleeved on the outside of the threaded rod, connecting plates being fixedly provided at both ends of the threaded sliders, and symmetrically distributed inclined plates being fixedly provided on the outside of the connecting plates, the inclined plates being movably inserted in the clamping blocks.

[0006] Preferably, a refrigeration component is fixedly provided on the outer side of the machine body, an annular tube is installed on the inner wall of the machine body, the annular tube is located between the limiting plate and the bottom plate, the output end of the refrigeration component is connected to both ends of the annular tube respectively, and a heat insulation layer is fixedly provided on the inner wall of the machine body.

[0007] Preferably, the bottom of the machine body is fixedly provided with a base arranged in an array, the top of the machine body is rotatably hinged with a cover plate, a sealing ring is fixedly provided on the side of the cover plate near the machine body, and a handle is fixedly provided on the end of the cover plate away from the sealing ring.

[0008] Preferably, the limiting plate has an array of insertion holes, a motor is fixedly mounted on the outside of the base plate, the end of the motor output shaft is inserted through the base plate and fixedly connected to the threaded rod, and insertion strips are fixedly mounted on both sides of the clamping block, the insertion strips being movably inserted into the external slide rail.

[0009] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0010] 1. In this utility model, by setting up a machine body, an external slide, a clamping block, a threaded rod, a threaded slider, a connecting plate and an inclined plate, etc., the structure can be used to clamp and position centrifuge tubes of different specifications, thereby facilitating the centrifugation separation of centrifuge tubes of different specifications and thus improving the application range of the device.

[0011] 2. In this utility model, by setting up a cooling component, a ring tube and a heat insulation layer and other structures in cooperation, the temperature of the centrifuge tube can be kept constant during the centrifugation of nucleic acid, so as to prevent nucleic acid degradation and maintain enzyme activity, thereby improving the efficiency of centrifugation. Attached Figure Description

[0012] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a side view of the overall structure of this utility model;

[0015] Figure 3 This is a schematic cross-sectional view of the body and connecting structure of this utility model;

[0016] Figure 4 This is a schematic diagram of the base plate and its connection structure of the present invention;

[0017] Figure 5 This is a schematic diagram of the positioning component structure of this utility model.

[0018] In the diagram: 1. Body; 11. Drive assembly; 12. Base plate; 13. Cover plate; 14. Sealing ring; 15. Handle; 16. Base; 17. Limiting plate; 18. Insertion hole; 2. Positioning assembly; 21. External slide rail; 22. Clamping block; 23. Insert strip; 24. Threaded rod; 25. Threaded slider; 26. Connecting plate; 27. Inclined plate; 28. Motor; 3. Refrigeration assembly; 31. Circular tube; 32. Insulation layer; 4. Centrifuge tube. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example: Figure 1-5 As shown, this utility model provides a rapid centrifugation device for nucleic acid extraction, including a body 1 and a drive assembly 11. The drive assembly 11 is composed of an electric motor, which drives the base plate 12 to rotate. The drive assembly 11 is installed inside the body 1, and the base plate 12 is connected to the outside of the drive assembly 11. A limiting disk 17 is fixed at the top of the base plate 12, which can limit the centrifuge tube 4. An array of positioning components 2 is fixed at the top of the drive assembly 11. The positioning components 2 include an external slide 21. The slide 21 is fixedly installed on the top of the base plate 12. The clamping blocks 22 are symmetrically distributed and movably inserted in the external slide 21. The top of the base plate 12 is rotatably provided with a threaded rod 24. The bottom end of the threaded slider 25 is attached to the top of the base plate 12. Three threaded sliders 25 are threadedly sleeved on the outside of the threaded rod 24. The two ends of the threaded sliders 25 are respectively fixed with connecting plates 26. The outside of the connecting plates 26 is fixed with symmetrically distributed inclined plates 27. The inclined plates 27 are movably inserted in the clamping blocks 22. The movement of the inclined plates 27 can drive the two clamping blocks 22 to move in opposite directions.

[0021] A refrigeration component 3 is fixedly installed on the outer side of the body 1, and an annular tube 31 is installed on the inner wall of the body 1. The annular tube 31 is located between the limiting plate 17 and the base plate 12. The output end of the refrigeration component 3 is connected to both ends of the annular tube 31 respectively. An insulation layer 32 is fixedly installed on the inner wall of the body 1.

[0022] By adopting the above technical solution, the refrigeration component 3 is composed of a compressor and a heat exchanger, which can circulate liquid nitrogen or coolant in the annular tube 31, thereby cooling the centrifuge tube 4 to prevent nucleic acid degradation and maintain enzyme activity.

[0023] The bottom of the body 1 is fixed with bases 16 arranged in an array.

[0024] By adopting the above technical solution, the base 16 can support the entire device.

[0025] The top of the body 1 is hinged to a cover plate 13.

[0026] By adopting the above technical solution, the cover plate 13 can seal the body 1.

[0027] A sealing ring 14 is fixedly provided on the side of the cover plate 13 near the body 1, and a handle 15 is fixedly provided on the end of the cover plate 13 away from the sealing ring 14.

[0028] By adopting the above technical solution, the sealing ring 14 can improve the sealing performance between the cover plate 13 and the body 1.

[0029] The limiting plate 17 has an array of sockets 18.

[0030] By adopting the above technical solution, the insertion hole 18 can limit the centrifuge tube 4.

[0031] A motor 28 is fixedly installed on the outside of the base plate 12. The end of the output shaft of the motor 28 is inserted through the base plate 12 and fixedly connected to the threaded rod 24.

[0032] By adopting the above technical solution, the output end of the motor 28 rotates, driving the threaded rod 24 to rotate.

[0033] Both sides of the clamping block 22 are fixedly provided with insert strips 23, which are movably inserted into the external slide rail 21.

[0034] By adopting the above technical solution, the insert 23 can be inserted into the external slide 21 to assist the clamping block 22 in moving.

[0035] Working principle: First, the solution containing the nucleic acid sample is placed in the centrifuge tube 4. Then, the centrifuge tube 4 is inserted into the insertion hole 18 in the limiting plate 17. Next, the motor 28 is started, causing the output end of the motor 28 to rotate, which in turn rotates the threaded rod 24. The rotation of the threaded rod 24 causes three threaded sliders 25 to move above the base plate 12. The movement of the threaded sliders 25 causes the connecting plate 26 to move, which in turn causes the inclined plate 27 to move. The movement of the inclined plate 27 causes two clamping blocks 22 to move towards the centrifuge tube 4 within the external slide rail 21, thus clamping and fixing the centrifuge tube 4. The device can clamp and position centrifuge tubes 4 of different sizes, thus facilitating centrifugation of different sizes of centrifuge tubes 4 and improving the applicability of the device. Then, the cover plate 13 can be closed, and the base plate 12 can be rotated by the drive component 11, so that nucleic acid samples can be extracted and centrifuged quickly. During the separation process, the cooling component 3 and the ring tube 31 can cool the area around the centrifuge tube 4. Then, the heat insulation layer 32 can prevent heat loss, so that the temperature of the centrifuge tube 4 is kept constant to prevent nucleic acid degradation and maintain enzyme activity, thereby improving the efficiency of centrifugation.

[0036] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A rapid centrifugation device for nucleic acid extraction, comprising a body (1) and a drive assembly (11), characterized in that: The drive assembly (11) is installed inside the body (1). The drive assembly (11) is connected to the base plate (12) on the outside. The base plate (12) is fixedly provided with a limiting plate (17) at the top. The drive assembly (11) is fixedly provided with a positioning assembly (2) arranged in an array at the top. The positioning assembly (2) includes an external slide rail (21). The external slide rail (21) is fixedly installed at the top of the base plate (12). A clamping block (22) arranged in a symmetrical arrangement is movably inserted in the external slide rail (21). A threaded rod (24) is rotatably provided at the top of the base plate (12). Three threaded sliders (25) are threadedly sleeved on the outside of the threaded rod (24). A connecting plate (26) is fixedly provided at both ends of the threaded slider (25). An inclined plate (27) arranged in a symmetrical arrangement is fixedly provided on the outside of the connecting plate (26). The inclined plate (27) is movably inserted in the clamping block (22).

2. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: A refrigeration component (3) is fixedly provided on the outside of the body (1), and an annular tube (31) is installed on the inner wall of the body (1). The annular tube (31) is located between the limiting plate (17) and the bottom plate (12). The output end of the refrigeration component (3) is connected to both ends of the annular tube (31). A heat insulation layer (32) is fixedly provided on the inner wall of the body (1).

3. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: The bottom of the body (1) is fixed with a base (16) arranged in an array.

4. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: The top of the body (1) is hinged to a cover plate (13).

5. The rapid centrifugation device for nucleic acid extraction as described in claim 4, characterized in that: A sealing ring (14) is fixedly provided on the side of the cover plate (13) near the body (1), and a handle (15) is fixedly provided on the end of the cover plate (13) away from the sealing ring (14).

6. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: The limiting plate (17) has an array of insertion holes (18).

7. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: A motor (28) is fixedly installed on the outside of the base plate (12). The output shaft end of the motor (28) is inserted through the base plate (12) and fixedly connected to the threaded rod (24).

8. The rapid centrifugation device for nucleic acid extraction as described in claim 1, characterized in that: Both sides of the clamping block (22) are fixedly provided with inserts (23), and the inserts (23) are movably inserted into the external slide (21).

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