Feeding pipe assembly, fermentation feeding bottle cap and feeding bottle

Abstract

The utility model provides a material supplementing pipe assembly, a fermentation material supplementing bottle cap and a material supplementing bottle, the material supplementing pipe assembly comprises a first connecting pipe and a second connecting pipe, the first connecting pipe is provided with a steam inlet and a steam outlet, the steam inlet and the steam outlet are oppositely arranged at the two ends of the first connecting pipe, and the second connecting pipe is provided with a steam outlet. The steam inlet is used for introducing high-temperature steam; the first connecting pipe comprises a first end and a second end, and the first end of the first connecting pipe is communicated with the second connecting pipe; the second end of the first connecting pipe is communicated with a connector of the fermentation tank; the high-temperature steam is introduced into the first connecting pipe through the steam inlet so as to sterilize the connector of the fermentation tank. The connector of the fermentation tank is subjected to high-temperature sterilization through the material supplementing pipe assembly, so that the material supplementing bottle can be connected to the fermentation tank in the middle of the fermentation process, and the problem that a conventional material supplementing bottle cannot be connected in the middle of the fermentation process and cannot be sterilized at the connector is solved.

Description

Technical Field

[0001] This application belongs to the field of fermenter feeding technology, specifically relating to a feeding pipe assembly, a fermentation feeding bottle cap, and a feeding bottle. Background Technology

[0002] Feeding bottles are containers used to replenish nutrients, adjust pH, or add specific substances to the fermentation system during the fermentation process. They are usually made of materials such as glass, plastic, and stainless steel and need to be used in conjunction with a peristaltic pump to achieve the feeding operation.

[0003] Currently, most feed bottles are formed by combining a blue-capped bottle with a 0.22μm microporous filter membrane and a silicone tube. They are simple to manufacture and highly usable. However, they still show shortcomings in certain application scenarios. Specifically, conventional feed bottles need to be sterilized at high temperature in an autoclave before being connected to the fermentation system in the fermenter before the materials in the fermenter are sterilized. If the feed bottle is connected during fermentation, the interface of the fermenter is exposed to the indoor environment and is in a state of contamination. Therefore, connecting the feed bottle to the interface of the fermenter during fermentation cannot guarantee a completely sterile experimental environment for the fermentation system. Utility Model Content

[0004] Therefore, the purpose of this application is to provide a feeding tube assembly, a fermentation feeding bottle cap, and a feeding bottle, so as to solve at least one technical problem existing in the prior art.

[0005] To address the aforementioned problems, a first aspect of this application provides a feed pipe assembly, comprising a first connecting pipe and a second connecting pipe. The first connecting pipe is provided with a steam inlet and a steam outlet, which are arranged opposite to each other at both ends of the first connecting pipe. The steam inlet is used to introduce high-temperature steam. The first connecting pipe includes a first end and a second end, with the first end of the first connecting pipe communicating with the second connecting pipe. The second end of the first connecting pipe is communicated with the interface of the fermenter. The high-temperature steam is introduced into the first connecting pipe through the steam inlet to sterilize the interface of the fermenter.

[0006] Optionally, a first valve body is installed on the steam inlet, and a second valve body is installed on the steam outlet.

[0007] Optionally, the first connecting pipe is provided with a third valve body and a fourth valve body, wherein the third valve body is closer to the first end than the first valve body, and the fourth valve body is farther away from the second end than the second valve body.

[0008] In a second aspect, this application provides a fermentation feed bottle cap, which is used to be disposed on the feed tube assembly described in any one of the above claims.

[0009] Optionally, the fermentation feed bottle cap includes a cap body and a fixing plate. The cap body includes a receiving cavity, and the fixing plate is disposed in the receiving cavity. Multiple rigid tubes are inserted through the fixing plate. The multiple rigid tubes are arranged close to the edge of the cap body, and one end of each of the multiple rigid tubes extends to the outside of the top of the cap body. One of the rigid tubes is connected to the feed tube assembly.

[0010] Optionally, the plurality of rigid tubes are a first rigid tube, a second rigid tube, and a third rigid tube. The first rigid tube is used to install a temperature measuring element, the second rigid tube is used to balance the air pressure, and the third rigid tube is used to transport the liquid material.

[0011] Optionally, the fermentation feed bottle cap further includes a gasket disposed within the receiving cavity of the cap body.

[0012] In a third aspect, this application provides a feeding bottle, comprising a bottle body, a feeding tube assembly as described in any one of the above claims, and a fermentation feeding bottle cap as described in any one of the above claims, wherein the fermentation feeding bottle cap is fitted onto the bottle body, and the feeding tube assembly is connected to the fermentation feeding bottle cap.

[0013] Optionally, the bottle body includes an inner bottle body and an outer bottle body, the outer bottle body is fitted over the outer side of the inner bottle body, and a gap is provided between the outer bottle body and the inner bottle body to form a cavity.

[0014] Optionally, the outer bottle body is provided with graduations along its length.

[0015] By employing the above technical solution, this utility model application has at least the following beneficial effects:

[0016] This application provides a feeding pipe assembly. High-temperature steam is introduced into the first connecting pipe through a steam inlet to sterilize the interface of the fermenter. The sterilized steam is discharged from the steam outlet and then fed into the fermenter. By sterilizing the interface of the fermenter through the feeding pipe assembly, the feeding bottle can be connected to the fermenter during the fermentation process, thus solving the problem that conventional feeding bottles cannot be connected during the fermentation process and cannot solve the problem of sterilization at the interface.

[0017] This application provides a fermentation feeding bottle cap. By setting multiple rigid tubes on the fixing plate of the fermentation feeding bottle cap, and ensuring that the rigid tubes are located at the edge of the fixing plate, when the feeding material contains insoluble substances such as soybean cake and requires magnetic stirring inside, the rigid tubes are not affected by the funnel-shaped vortex, and can smoothly suck the material into the suction pipe for normal feeding during the feeding process.

[0018] This application provides a feeding bottle with an inner and outer chamber. The bottle body has two layers to form a chamber. Ice is added into the chamber through the inlet to cool the liquid. This method is suitable for cooling the liquid when inoculating seed liquid into the fermenter by feeding method, while the seed liquid is waiting for the fermenter to finish sterilization. This ensures the activity of the seed liquid and avoids the aging of the seed liquid. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the replenishment bottle structure according to an embodiment of this application;

[0020] Figure 2 This is a schematic diagram of the bottle body structure according to an embodiment of this application;

[0021] Figure 3 This is a schematic diagram of the fermentation feed bottle cap structure according to an embodiment of this application;

[0022] Figure 4 This is a schematic diagram of the central funnel-shaped vortex formed during the stirring process of the liquid in the feeding bottle according to an embodiment of this application.

[0023] The reference numerals in the attached figures are as follows:

[0024] 101. First connecting pipe; 102. Second connecting pipe; 103. Steam inlet; 104. Steam outlet; 105. First valve body; 106. Second valve body; 107. Third valve body; 108. Fourth valve body;

[0025] 201. Cover; 202. Fixing plate; 203. Gasket; 204. Pressure balance tube; 205. Suction tube;

[0026] 301. Inner bottle body; 302. Outer bottle body; 303. Inlet; 304. Drain outlet; 305. Chamber;

[0027] 401. Whirlpool. Detailed Implementation

[0028] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0029] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0030] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0031] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0032] See also Figures 1 to 4 As shown, according to a first aspect of an embodiment of this application, a feed pipe assembly is provided for use in a fermenter. The feed pipe assembly includes a first connecting pipe 101 and a second connecting pipe 102. The first connecting pipe 101 is provided with a steam inlet 103 and a steam outlet 104, which are arranged opposite to each other at both ends of the first connecting pipe 101. The steam inlet 103 is used to introduce high-temperature steam. The first connecting pipe 101 includes a first end and a second end. The first end of the first connecting pipe 101 is connected to the second connecting pipe 102. The second end of the first connecting pipe 101 is connected to the interface of the fermenter. High-temperature steam is introduced into the first connecting pipe 101 through the steam inlet 103 to sterilize the interface of the fermenter.

[0033] High-temperature steam is introduced into the first connecting pipe 101 through steam inlet 103 to sterilize the interface of the fermenter. The sterilized steam is discharged from steam outlet 104 and then fed into the fermenter. The feeding pipe assembly sterilizes the interface of the fermenter at high temperature, so that the feeding bottle can be connected to the fermenter during the fermentation process.

[0034] The second connecting pipe 102 is a high-temperature resistant silicone hose, and the peristaltic pump is installed on the second connecting pipe 102 to realize automatic feeding operation.

[0035] The first connecting pipe 101 is a stainless steel pipe. When the first connecting pipe 102 is connected to the second connecting pipe 101, the second connecting pipe 102 is fitted onto the end of the first connecting pipe 102, and the connection between the two is tightened by a clamp.

[0036] In this embodiment, the steam inlet 103 and the steam outlet 104 are arranged opposite to each other at the two ends of the first connecting pipe 101. That is, in this embodiment, the steam inlet 103 is arranged at the first end of the first connecting pipe 101, and the steam outlet 104 is arranged at the second end of the first connecting pipe 101.

[0037] The first connecting pipe 101 is equipped with a temperature sensor and a pressure sensor. The temperature sensor monitors the temperature of the incoming high-temperature steam in real time to determine the sterilization time of the fermenter interface. The pressure sensor monitors the pressure inside the first connecting pipe 101 to ensure that the pressure inside the first connecting pipe 101 reaches the pressure value corresponding to the sterilization temperature. The pressure value inside the pipe is directly proportional to the temperature value of the high-temperature steam.

[0038] In this embodiment, the temperature of the high-temperature steam inside the first connecting pipe 101 is 121°C, and the corresponding pressure inside the first connecting pipe 101 is 0.115 MPa; at this time, the sterilization time at the fermenter interface is 20-30 minutes. In other embodiments, the temperature, pressure, and sterilization time of the high-temperature steam inside the pipe can be adjusted according to actual conditions.

[0039] The feed tube assembly is connected at both ends to the feed bottle and the fermenter, respectively. The specific usage process is as follows:

[0040] When the feed bottle is connected before the fermenter is sterilized, the feed can be added by opening the third valve body 107 and the fourth valve body 108 after the fermenter is sterilized.

[0041] When temporary feeding is required during the fermentation process in the fermenter, after connecting the feeding bottle, open the first valve body 105, the second valve body 106, and the fourth valve body 108. High-temperature steam enters the first connecting pipe 101 from the steam inlet 103 to sterilize the interface of the fermenter at high temperature. The sterilized steam is discharged from the steam outlet 104. After steam sterilization for 30 minutes, close the first valve body 105 and the second valve body 106; then open the third valve body 107 to feed the fermenter.

[0042] In this application, the sterilization time is 30 minutes. In other embodiments, the sterilization time can be adjusted according to the specific circumstances.

[0043] In other embodiments, a first valve body 105 is installed on the steam inlet 103, and a second valve body 106 is installed on the steam outlet 104.

[0044] The first valve body 105 is used to control whether steam enters the first connecting pipe 101. When steam is needed, the first valve body 105 is opened, and high-temperature steam enters the first connecting pipe 101 through the steam inlet 103; when steam is not needed, the first valve body 105 is closed to cut off the steam supply.

[0045] By controlling the opening of the second valve body 106, the speed at which high-temperature steam is discharged from the steam outlet 104 can be slowed down, so as to prolong the residence time of high-temperature steam at the fermenter interface and sterilize the fermenter interface at high temperature.

[0046] In other embodiments, a third valve body 107 and a fourth valve body 108 are provided on the first connecting pipe 101. The third valve body 107 is closer to the first end than the first valve body 105, and the fourth valve body 108 is farther away from the second end than the second valve body 106. By opening the third valve body 107 and the fourth valve body 108, the feed liquid can be introduced into the fermenter.

[0047] The third valve body 107 is closer to the first end than the first valve body 105, meaning that during installation, the third valve body 107 is arranged closer to the first end of the first connecting pipe 101; the fourth valve body 108 is farther from the second end than the second valve body 106, meaning that during installation, the second valve body 106 is arranged closer to the second end of the first connecting pipe 101.

[0048] A second aspect of this application provides a fermentation feed bottle cap, which is used to be disposed on the feed tube assembly of any of the above-mentioned embodiments.

[0049] The fermentation feeding bottle cap is used to be installed on the feeding tube assembly of any of the above items. That is to say, the feeding tube assembly is connected to the fermentation feeding bottle cap through the second connecting tube 102 thereon.

[0050] In other embodiments, the fermentation feeding bottle cap includes a cap body 201 and a fixing piece 202. The cap body 201 includes a receiving cavity, and the fixing piece 202 is disposed in the receiving cavity. Multiple rigid tubes are inserted through the fixing piece 202. The multiple rigid tubes are arranged close to the edge of the cap body 201, and one end of each of the multiple rigid tubes extends to the outside of the top of the cap body 201. One of the rigid tubes is connected to the feeding tube assembly.

[0051] Multiple rigid tubes are arranged on the fixed plate 202, and multiple rigid tubes are arranged close to the edge of the cap 201. When the feeding material contains insoluble substances such as soybean cake and magnetic stirring is required inside, the suction tube inside the traditional blue cap feeding bottle is a flexible tube, which is easily affected by the funnel-shaped vortex 401 and causes circumferential oscillation. During the feeding process, the material cannot enter the suction tube and thus affects the normal feeding.

[0052] In other embodiments, the multiple rigid tubes are a first rigid tube, a second rigid tube, and a third rigid tube. The first rigid tube is used to install a temperature measuring element, the second rigid tube is used to balance the air pressure, and the third rigid tube is used to transport the liquid.

[0053] The first rigid tube is used to mount the temperature measuring element. Specifically, the measuring end of the temperature measuring element enters from the end of the first rigid tube located outside the cover 201 and is fixed to the other end of the first rigid tube extending into the liquid to obtain the temperature of the liquid. Furthermore, under magnetic stirring, due to the presence of the first rigid tube, the temperature measuring element is unaffected by the magnetic stirring and always maintains its fixed installation position.

[0054] Specifically, in this embodiment, the temperature measuring element is a thermometer.

[0055] The second rigid tube is used to balance the air pressure. Specifically, one end of the second rigid tube located outside the cap 201 is connected to the microporous filter membrane to enable the second rigid tube to communicate with the outside world, ensuring that the air pressure inside and outside the replenishment bottle is balanced. The microporous filter membrane filters the outside air to prevent bacterial contamination inside the replenishment bottle.

[0056] Specifically, in this embodiment, the pore diameter of the microporous filter membrane is 0.22 μm.

[0057] In other embodiments, the fermentation feed bottle cap further includes a gasket 203, which is disposed within the receiving cavity of the cap body 201. The arrangement of the gasket 203 ensures the sealing of the connection between the fermentation feed bottle cap and the feed bottle.

[0058] A third aspect of the embodiments of this application provides a feeding bottle, including a bottle body, a feeding tube assembly of any one of the above-mentioned components, and a fermentation feeding bottle cap of any one of the above-mentioned components. The fermentation feeding bottle cap is closed on the bottle body, and the feeding tube assembly is connected to the fermentation feeding bottle cap.

[0059] In other embodiments, the bottle body includes an inner bottle body 301 and an outer bottle body 302, with the outer bottle body 302 fitted over the outer side of the inner bottle body 301, and a spacer is provided between the outer bottle body 302 and the inner bottle body 301 to form a chamber 305.

[0060] Here, the space formed by the inner bottle body 301 is used to hold the liquid. After the fermentation feed bottle cap is fastened to the bottle body, the first rigid tube and the third rigid tube on the fermentation feed bottle cap extend into the liquid.

[0061] The outer bottle body 302 is fitted onto the outside of the inner bottle body 301. The inner bottle body 301 includes an open end that extends out of the outer bottle body 302. The outer circumferential surface of the portion of the inner bottle body 301 extending out of the outer bottle body 302 is machined with external threads to achieve a threaded connection with the fermentation feed bottle cap.

[0062] The outer bottle 302 and the inner bottle 301 are separated by a gap to form a chamber 305. The chamber 305 is filled with ice to cool the liquid in the inner bottle 301. This can be used when the seed liquid is inoculated into the fermenter by the feeding method. During the time (about 1 hour) that the seed liquid is waiting for the fermenter to finish sterilization, the seed liquid is cooled to ensure the activity of the strain and avoid strain aging.

[0063] Specifically, the outer bottle body 302 includes an open end, which bends towards the inner bottle body 301 and is integrated with the inner bottle body 302. After bending, the outer bottle body 302 forms a platform, and an inlet 303 is provided on the platform of the outer bottle body 302, through which slush ice is introduced into the chamber 305.

[0064] The bottom side wall of the outer bottle body 302 is provided with a drain outlet 304, through which the slush in the chamber 305 is discharged; normally, the slush in the chamber 305 is discharged through the drain outlet 304 after it has melted into water.

[0065] Specifically, the inner bottle body 301 and the outer bottle body 302 can be made of glass or high-temperature resistant plastic.

[0066] In other embodiments, the inner bottle 301 is provided with graduations along its length. The volume of the liquid inside the inner bottle 301 can be determined by the graduations.

[0067] It will be readily understood by those skilled in the art that the aforementioned advantageous methods can be freely combined and superimposed without conflict.

[0068] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application. The above are merely preferred embodiments of this application. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this application, and these improvements and modifications should also be considered within the protection scope of this application.

Claims

1. A fill pipe assembly, characterized by, The feeding pipe assembly comprises: a first connecting pipe (101) provided with a steam inlet (103) and a steam outlet (104) arranged at opposite ends of the first connecting pipe (101), the steam inlet (103) being used for passing in high-temperature steam; a second connecting pipe (102), the first end of the first connecting pipe (101) being in communication with the second connecting pipe (102), and the second end of the first connecting pipe (101) being in communication with an interface of a fermentation tank; The high-temperature steam is passed into the first connecting pipe (101) through the steam inlet (103) to sterilize the interface of the fermentation tank.

2. A filler pipe assembly according to claim 1, wherein A first valve body (105) is installed on the steam inlet (103), and a second valve body (106) is installed on the steam outlet (104).

3. A filler pipe assembly according to claim 2, wherein, A third valve body (107) and a fourth valve body (108) are arranged on the first connecting pipe (101), the third valve body (107) being closer to the first end than the first valve body (105), and the fourth valve body (108) being farther away from the second end than the second valve body (106).

4. A fermentation feed bottle cap characterized by, The fermentation feeding bottle cap is arranged on the feeding pipe assembly of any one of claims 1 to 3.

5. A fermentation feed bottle cap according to claim 4, wherein, The fermentation feeding bottle cap comprises a cap body (201) and a fixing sheet (202), the cap body (201) comprising a containing cavity, and the fixing sheet (202) being arranged in the containing cavity; a plurality of hard pipe bodies are arranged on the fixing sheet (202), the plurality of hard pipe bodies being arranged close to the edge of the cap body (201), and one end of each of the plurality of hard pipe bodies extending to the outside of the cap top of the cap body (201), wherein one of the hard pipe bodies is in communication with the feeding pipe assembly.

6. A fermentation feed bottle cap according to claim 5, wherein, The plurality of hard pipe bodies are a first hard pipe body, a second hard pipe body and a third hard pipe body, the first hard pipe body being used for installing a temperature measuring element, the second hard pipe body being used for balancing air pressure, and the third hard pipe body being used for conveying liquid.

7. A fermentation feed bottle cap according to claim 5, wherein The fermentation feeding bottle cap further comprises a gasket (203) arranged in the containing cavity of the cap body (201).

8. A feeding bottle, characterized in that The fermentation feeding bottle cap comprises a bottle body, the feeding pipe assembly of any one of claims 1 to 3, and the fermentation feeding bottle cap of any one of claims 4 to 7, the fermentation feeding bottle cap being arranged on the bottle body, and the feeding pipe assembly being connected to the fermentation feeding bottle cap.

9. A feeding bottle according to claim 8, wherein The bottle body comprises an inner bottle body (301) and an outer bottle body (302), the outer bottle body (302) being arranged outside the inner bottle body (301), and a chamber (305) being formed between the outer bottle body (302) and the inner bottle body (301) by a spacing distance.

10. A feeding bottle according to claim 9, wherein The outer bottle body (302) is provided with a scale along the length direction.

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