Cell sedimentation device and cell purification system

Abstract

The utility model relates to a cell sedimentation device and a cell purification system. The cell sedimentation device comprises a sedimentation bag. The settling bag is provided with a first cavity and a second cavity. The settling bag is oppositely provided with a first side and a second side in the height direction of the settling bag, the first cavity is formed in the first side, and the second cavity is formed in the second side. And the settling bag is also provided with a liquid inlet, a liquid outlet and a sediment outlet. The liquid inlet communicates with the first cavity and is close to the first side. The liquid outlet communicates with the second cavity and is close to the second side. The sediment discharge port is communicated with the second cavity, the sediment discharge port is formed in the second side, and the sediment discharge port and the liquid outlet are arranged at an interval in the height direction. The inner side wall of the second cavity is gradually folded in the direction away from the first cavity. The cell sedimentation device disclosed by the utility model is beneficial to improving the recovery quality of a target product.

Description

Technical Field

[0001] This application relates to the technical field of cell sedimentation, and in particular to cell sedimentation devices and cell purification systems. Background Technology

[0002] In cell engineering processes such as retrovirus production, the following steps are typically involved: culturing the target system, collecting the target product, and final product acceptance. Among these, the collection of the target product is a crucial indicator affecting the final product acceptance result.

[0003] Taking the production process of retroviruses as an example, the collection of target products mainly refers to the collection of cell supernatant. In this process, the relevant technologies mainly adopt high-speed centrifugation and multi-stage filtration.

[0004] However, the two methods employed in the relevant technologies have the following shortcomings: Firstly, the high-speed centrifugation method involves excessively fast rotation speeds, which can easily cause cell damage and affect the quality of the finished product. Secondly, the multi-stage filtration method is prone to low product recovery rates due to operational errors and other factors. Utility Model Content

[0005] Therefore, it is necessary to provide a cell sedimentation device and a cell purification system to address the issue of how to improve the recycling quality of the target product.

[0006] A cell sedimentation device, the cell sedimentation device comprising:

[0007] A settling bag, wherein the settling bag has a first cavity and a second cavity; the settling bag has a first side and a second side opposite to each other along the height direction of the settling bag, the first cavity is disposed on the first side, the second cavity is disposed on the second side, and the first cavity and the second cavity are connected.

[0008] The settling bag is further provided with an inlet, an outlet, and a sediment discharge outlet; the inlet is connected to the first cavity and is located close to the first side; the outlet is connected to the second cavity and is located close to the second side; the sediment discharge outlet is connected to the second cavity and is located on the second side, and the sediment discharge outlet and the outlet are spaced apart along the height direction;

[0009] The inner wall of the second cavity is gradually narrowed in a direction away from the first cavity.

[0010] In one embodiment, the cell sedimentation device further includes a first output component; the first output component includes a first output tube; the first output tube is connected in a one-to-one correspondence with at least one of the liquid inlet and the liquid outlet, such that the first output tube is connected to the first cavity and the second cavity.

[0011] In one embodiment, the first output component further includes a first connector, which has a first end and a second end opposite to each other along its length; the first end is in one-to-one communication with at least one of the liquid inlet and the liquid outlet, and the second end is in a sealed connection with the first output tube.

[0012] In one embodiment, the first output component further includes a first flow regulator, which is disposed in the first output tube and is used to regulate the sample flow rate in the first output tube.

[0013] In one embodiment, the cell sedimentation device further includes a second output component, which includes a second output tube that is inserted into the sediment discharge outlet such that at least a portion of the second output tube is inserted into the second cavity.

[0014] In one embodiment, the end of the second output tube that is inserted into the sediment discharge port is provided with a tapered inlet structure, and the tapered inlet structure is gradually tapered away from the second cavity.

[0015] In one embodiment, the second output component further includes a second connecting connector, which includes a connector body and a clamping ring; the connector body is provided with a third end and a fourth end opposite to each other along the length direction of the connector body, the third end is sleeved with the clamping ring so that the clamping ring is sleeved on the outer side wall of the connector body and the clamping ring can abut against the side edge of the sedimentation outlet; the fourth end is connected to the second output pipe.

[0016] In one embodiment, the second output component further includes a third connector and a third output tube. The third connector has a fifth end and a sixth end, and the extension direction of the fifth end intersects the extension direction of the sixth end. The fifth end is used to communicate with the second output tube, and the sixth end is used to communicate with the third output tube.

[0017] The settling bag has a first surface and a second surface opposite to each other along the thickness direction of the settling bag, the liquid outlet is located on the first surface, and the outer wall of the third output pipe is connected and cooperates with the second surface.

[0018] In one embodiment, the settling bag has a connecting portion on the second side, the connecting portion being used to connect and cooperate with the third output pipe; the orthographic projection area of ​​the connecting portion along the thickness direction coincides with the orthographic projection area of ​​the liquid outlet along the thickness direction;

[0019] And / or, the third output pipe is bonded to the outer surface of the settling bag, such that the outer wall of the third output pipe is bonded to the second surface.

[0020] A cell purification system includes a sample injection component, a sampling component, a sample receiving component, and the cell sedimentation device described in the above embodiments. The sample injection component is connected to the liquid inlet so that the sample injection component injects sample liquid into a first cavity through the liquid inlet. The sampling component is connected to the liquid outlet so that sample liquid can be injected into the sampling component from a second cavity. The sample receiving component is connected to the sedimentation outlet so that sample liquid is discharged from the second cavity to the sample receiving component.

[0021] The aforementioned cell sedimentation device and cell purification system can separate cell sample solutions using a sedimentation bag. The separation process is gentle and does not damage the cells, ensuring the accuracy of the final product. Furthermore, the gradual narrowing of the inner wall of the second cavity away from the first cavity helps to slow down the flow rate of the sample solution in both the first and second cavities, allowing for thorough phase separation of the supernatant and the lower turbid liquid. This improves the separation quality of the supernatant and optimizes the recovery quality of the final product.

[0022] Furthermore, by setting the sediment discharge port and liquid outlet alternately, the sediment discharge process and the liquid outlet do not interfere with each other. This prevents sediment adhering to the sediment discharge port wall from being flushed out into the sampling component by the supernatant when the sediment discharge port and liquid outlet are set together. Since the amount of supernatant sample taken depends to a maximum extent on the height of the liquid outlet, and there is a certain height difference between the liquid outlet and the sediment discharge port, the phase separation boundary will eventually be lower than the liquid outlet, ensuring that the sample taken from the liquid outlet is the supernatant, thus guaranteeing the quality of the supernatant. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the cell sedimentation device in one embodiment.

[0024] Figure 2 for Figure 1 The diagram shows an explosion of the cell sedimentation device.

[0025] Explanation of reference numerals in the attached figures:

[0026] 100. Cell sedimentation device; 110. Sedimentation bag; 110a. First cavity; 110b. Second cavity; 111. First side; 112. Second side; 113. Inlet; 114. Outlet; 115. Sediment discharge outlet; 116. Connecting part; 120. First output assembly; 121. First output pipe; 122. First connecting joint; 123. First flow regulator; 130. Second output assembly; 131. Second output pipe; 131a. Conical inlet structure; 132. Second connecting joint; 1321. Joint body; 1322. Clamping ring; 133. Third connecting joint; 134. Third output pipe; 140. Lifting component; 150. Baffle plate component; 160. Fixing component; X, height direction. Detailed Implementation

[0027] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0028] This application provides a cell purification system, including a cell sedimentation device 100, a sample injection component, a sampling component, and a sample receiving component.

[0029] See Figure 1 , Figure 1 A schematic diagram of a cell sedimentation device 100 according to an embodiment of this application is shown. The cell sedimentation device 100 provided in this embodiment includes a sedimentation bag 110. Specifically, the sedimentation bag 110 is provided with a first cavity 110a and a second cavity 110b. The sedimentation bag 110 is provided with a first side 111 and a second side 112 opposite to each other along the height direction X of the sedimentation bag 110. The first cavity 110a is disposed on the first side 111, and the second cavity 110b is disposed on the second side 112. The first cavity 110a and the second cavity 110b are connected.

[0030] Combination Figure 1 as well as Figure 2As shown, the settling bag 110 is also provided with an inlet 113, an outlet 114, and a sediment discharge outlet 115. The inlet 113 is connected to the first cavity 110a and is located near the first side 111. The outlet 114 is connected to the second cavity 110b and is located near the second side 112. The sediment discharge outlet 115 is connected to the second cavity 110b and is located on the second side 112. The sediment discharge outlet 115 and the outlet 114 are spaced apart along the height direction X. The inner wall of the second cavity 110b gradually narrows away from the first cavity 110a.

[0031] The sample injection assembly is connected to the inlet 113 to inject sample solution into the first cavity 110a through the inlet 113. The sampling assembly is connected to the outlet 114 to inject sample solution from the second cavity 110b into the sampling assembly. The sample receiving assembly is connected to the sediment discharge outlet 115 to discharge sample solution from the second cavity 110b to the sample receiving assembly.

[0032] To facilitate understanding, the following detailed explanation is provided in conjunction with the working process of the cell sedimentation device 100.

[0033] Understandably, after the sample solution enters the first cavity 110a, it gradually fills both the first cavity 110a and the second cavity 110b. As the settling time increases, due to the density difference, the sample solution will separate into supernatant and turbid liquid. The turbid liquid will include a mixture of precipitate and other liquids. The supernatant is concentrated in the first cavity 110a, while the turbid liquid is concentrated in the second cavity 110b, and the precipitate will accumulate at the bottom of the second cavity 110b.

[0034] The sample solution undergoes the following main processing steps in the cell sedimentation device 100, as detailed below:

[0035] (1) Liquid inlet: The sample liquid is injected into the sedimentation bag 110 through the liquid inlet 113.

[0036] (2) Static settling: The settling bag 110 is suspended and left to stand. At this time, the sample solution flows in the settling bag 110 and the cells naturally settle to the bottom of the settling bag 110 (i.e., the location of the second side 112).

[0037] (4) Removal of sediment: A clear solid-liquid interface is formed in the sedimentation bag 110, indicating that sedimentation is complete. At this time, the sediment can be discharged through the sedimentation outlet 115, and the liquid level of the supernatant gradually decreases.

[0038] (5) Discharge: When the sample liquid of the supernatant is flush with the outlet 114, the supernatant can be discharged through the outlet 114 to complete the supernatant transfer process.

[0039] Thus, the separation of cell sample solution can be completed through the sedimentation bag 110. The separation process is gentle and will not damage the cells, ensuring the detection accuracy of the finished product. Furthermore, the inner wall of the second cavity 110b is gradually narrowed away from the first cavity 110a, which helps to slow down the flow rate of the sample solution in the first cavity 110a and the second cavity 110b. This allows the supernatant and the lower turbid liquid to achieve sufficient phase separation, thereby improving the separation quality of the supernatant and optimizing the recovery quality of the finished product.

[0040] Furthermore, by setting the sediment discharge port 115 and the liquid outlet 114 alternately, the sediment discharge process and the liquid outlet 114 do not interfere with each other. This prevents sediment adhering to the wall of the sediment discharge port 115 from being flushed out into the sampling component by the supernatant when the sediment discharge port 115 and the liquid outlet 114 are set together. Since the amount of supernatant sample taken depends to a maximum extent on the height of the liquid outlet 114, and there is a certain height difference between the liquid outlet 114 and the sediment discharge port 115, the phase separation boundary will eventually be lower than the liquid outlet 114, ensuring that the sample taken from the liquid outlet 114 is the supernatant, thus ensuring the quality of the supernatant.

[0041] The settling bag 110 is made of a therapeutically acceptable flexible material. A flexible material is understood to be deformable when subjected to pressure or stress, for example, due to filling and shaking of the bag. Specifically, the settling bag 110 may be made of conventional medical or pharmaceutical-grade polyvinyl chloride (PVC).

[0042] In one example, the settling bag 110 may be made of two thin sheets of flexible material, which are stacked together and their edges are welded together to form a first cavity 110a and a second cavity 110b communicating with an inlet 113, an outlet 114, and a sediment discharge outlet 115. Optionally, to prevent the first cavity 110a and the second cavity 110b of the settling bag 110 from being contaminated by chemicals, the sheets are ultrasonically sealed together.

[0043] In some embodiments, the sedimentation bag 110 has a translucent portion located near at least one of the outlet 114 and the sediment discharge outlet 115. This facilitates observation of the cell sedimentation process and allows for control of the sampling and sediment discharge process, improving the quality of the supernatant. Optionally, the sedimentation bag 110 is made of a translucent material.

[0044] To improve the stability of the sampling process, in some embodiments, such as Figure 2As shown, the cell sedimentation device 100 also includes a first output component 120. The first output component 120 includes a first output tube 121. The first output tube 121 is connected to at least one of the liquid inlet 113 and the liquid outlet 114, such that the first output tube 121 is connected to the first cavity 110a and the second cavity 110b.

[0045] In one example, the first output tube 121 is connected to the inlet 113. The first output tube 121 is used to connect the sample injection component so that the sample injection component can deliver liquid into the first cavity 110a through the first output tube 121.

[0046] In another example, the first output tube 121 is connected to the liquid outlet 114. The first output tube 121 is used to connect the sampling component so that the sampling component can obtain the supernatant in the second cavity 110b through the second output tube 131.

[0047] Thus, the first output tube 121 is designed to allow for sample introduction or extraction through a closed pipeline, which helps reduce the risk of external contamination. Furthermore, the inlet 113 and outlet 114 are connected by an independent first output tube 121 to avoid cross-path input / output (e.g., using different pipelines for sample introduction and extraction), ensuring sample purity.

[0048] Furthermore, to improve the stability of sample injection or sampling, in some embodiments, the first output component 120 also includes a first flow regulator 123. The flow regulator is disposed in the first output tube 121 and is used to regulate the sample flow rate within the first output tube 121. Thus, through the flow regulator, the operator can precisely control the rate at which the sample enters or leaves the settling bag 110, allowing the operator to flexibly adjust the sample flow rate according to actual needs to adapt to different experimental requirements. This precise flow control helps ensure the accuracy and repeatability of experimental results.

[0049] Accordingly, in other embodiments, see back Figure 2 The cell sedimentation device 100 also includes a second output component 130, which includes a second output tube 131. The second output tube 131 is inserted into the sedimentation outlet 115, such that at least a portion of the second output tube 131 is inserted into the second cavity 110b.

[0050] Thus, the second output pipe 131 is plugged into the sediment discharge port 115. The plug-in design facilitates quick installation or disassembly, and is convenient for maintenance and cleaning. Furthermore, the plug-in connection forms a closed channel to prevent external contaminants from entering or sediment from leaking. In addition, the second output pipe 131 is directly inserted into the sedimentation area (second cavity 110b), shortening the sediment discharge path and improving operating efficiency.

[0051] Furthermore, in some implementations, such asFigure 2 As shown, the end of the second output pipe 131 that is inserted into the sediment discharge outlet 115 is provided with a tapered inlet structure 131a. The tapered inlet structure 131a is gradually tapered away from the second cavity 110b. This gradual tapering of the tapered inlet structure 131a ensures a tight fit with the sediment discharge outlet 115, reducing the possibility of liquid or sediment leakage. Furthermore, the tapering design allows the sediment to flow in a concentrated manner during discharge, reducing sediment residue, improving discharge efficiency, and lowering the risk of pipe blockage.

[0052] Furthermore, in other embodiments, such as Figure 1 and Figure 2 As shown, the second output assembly 130 also includes a second connecting connector 132, which includes a connector body 1321 and a clamping ring 1322. The connector body 1321 has a third end and a fourth end opposite to each other along its length. The third end is fitted with the clamping ring 1322, so that the clamping ring 1322 is fitted onto the outer wall of the connector body 1321 and can abut against the side edge of the sedimentation outlet 115. The fourth end communicates with the second output pipe 131.

[0053] Thus, on the one hand, the insertion and mating of the connector body 1321 and the second output pipe 131 improves the connection strength and structural stability between the second output pipe 131 and the connector body 1321, reducing the risk of liquid or sediment leakage. On the other hand, the clamp ring 1322 is fitted on the outside of the connector body 1321 and abuts against the side edge of the sediment discharge port 115, which helps to prevent the second output pipe 131 from loosening or falling off during operation.

[0054] In one specific embodiment, the hardness of the connector body 1321 can be greater than the hardness of the second output tube 131. Thus, by making the hardness of the connector body 1321 greater than the hardness of the second output tube 131, the connection stability between the second output component 130 and the sedimentation outlet 115 can be strengthened, improving sedimentation stability during the sedimentation process and preventing sample leakage.

[0055] In conjunction with any embodiment of the second output component 130 described above, such as Figure 2 As shown, the second output assembly 130 also includes a third connector 133 and a third output tube 134. The third connector 133 has a fifth end and a sixth end, with the extension directions of the fifth end and the sixth end intersecting each other. The fifth end is used to communicate with the second output tube 131, and the sixth end is used to communicate with the third output tube 134.

[0056] The settling bag 110 has a first surface and a second surface opposite to each other along the thickness direction of the settling bag 110. The liquid outlet 114 is located on the first surface, and the outer wall of the third output pipe 134 is connected and cooperates with the second surface.

[0057] Thus, by having the fifth and sixth ends of the third connecting joint 133 intersect in their extending directions, space is fully utilized, the size of the device is reduced, and operation and storage are facilitated. Furthermore, the outer wall of the third output pipe 134 is connected to the second side of the settling bag 110, separating the liquid output path from the sediment discharge port 115 path, making observation more intuitive and avoiding obstruction of vision.

[0058] In addition, in one example, in conjunction with the embodiment in which the first output pipe 121 is connected to the liquid outlet 114 in the above embodiment, the third output pipe 134 is connected to the second side, so that the first output pipe 121 and the third output pipe 134 can be connected to opposite sides, thereby improving the center of gravity balance of the sedimentation bag 110 and avoiding insufficient purity of the supernatant separation (possibly mixed with precipitates) due to the tilt of the liquid surface during the separation process, which is conducive to improving the extraction quality of the supernatant.

[0059] Optionally, in one embodiment, the settling bag 110 has a connecting portion 116 on its second side, which is used to connect and cooperate with the third output pipe 134. The orthographic projection area of ​​the connecting portion 116 along the thickness direction coincides with the orthographic projection area of ​​the liquid outlet 114 along the thickness direction.

[0060] Thus, by aligning the orthographic projection area of ​​the connecting part 116 along the thickness direction with the orthographic projection area of ​​the outlet 114 along the thickness direction, the tensile force balance of the settling bag 110 can be ensured to the greatest extent, avoiding excessive collapse of the settling bag 110, thereby ensuring a clear distinction between the two-phase boundary liquid surface, which is beneficial to improving the separation and extraction quality of the supernatant and the precipitate.

[0061] It should be noted that the connection between the third output pipe 134 and the outer surface of the settling bag 110 can be, but is not limited to, bonding, clamping, etc., and no further restrictions are made here.

[0062] In one embodiment, the third output pipe 134 is bonded to the outer surface of the settling bag 110, such that the outer wall of the third output pipe 134 is bonded to the second surface. This bonded fit between the third output pipe 134 and the outer surface of the settling bag 110 helps maintain the flatness of the settling bag 110, thereby facilitating a clear distinction between the two-phase boundary liquid surfaces and improving the separation and extraction quality of the supernatant and precipitate.

[0063] In other implementations, such as Figure 2 As shown, the second output component 130 also includes a second flow regulator, which is disposed in the third output tube 134 and is used to regulate the sample flow rate in the third output tube 134.

[0064] It should be noted that the first flow regulator 123 and the second flow regulator in the above embodiments can be a throttling clamp (which changes the pipe diameter by clamping the pipe to change the flow velocity in the pipe) or a throttling valve, etc.

[0065] In conjunction with any embodiment of the second output component 130 described above, see back Figure 2 The second output assembly 130 also includes a sealing plate 150, which is used to seal the opening of the third output tube 134. When the sealing plate 150 is in closed engagement with the opening of the third output tube 134, the second cavity 110b is mutually sealed from the outside. Thus, the sealing plate 150 can effectively seal the opening of the third output tube 134 during supernatant extraction, thereby improving the stability of supernatant separation.

[0066] In some embodiments, see back Figure 1 The distance between the inlet 113 and the first side 111 is P1, where P1 = 5cm ~ 11cm. Within this range of P1 = 5cm ~ 11cm, the sample solution can be injected smoothly and evenly into the settling bag 110, avoiding problems such as uneven sample distribution or excessive local pressure caused by improper injection location. Simultaneously, this distance also ensures sufficient diffusion and settling of the sample solution within the settling bag 110.

[0067] In one embodiment, P1 = 7cm ~ 9cm. In another embodiment, P1 can be, but is not limited to, any one of 7cm, 8cm, 9cm, etc. Thus, this spacing allows the sample solution to diffuse more quickly to all corners of the sedimentation bag 110, enabling cells to be distributed more evenly during sedimentation, thereby reducing cell aggregation or uneven precipitation and promoting stable cell sedimentation and separation.

[0068] In other embodiments, see back Figure 1 The distance between the outlet 114 and the second side 112 is P2, where P2 = 15cm ~ 21cm.

[0069] Thus, with this spacing, the distance between the liquid outlet 114 and the sediment discharge outlet 115 is properly designed, ensuring that there is not too much residual sediment between the liquid outlet 114 and the sediment discharge outlet 115, as well as too much sample liquid near the boundary line. This ensures that the residual sample liquid in the sedimentation bag 110 is minimized, reducing the loss of supernatant and improving the recovery quality of supernatant.

[0070] Meanwhile, this spacing design ensures that the outlet 114 and the sediment discharge outlet 115 are sufficiently independent of each other, avoiding problems such as cell retention or poor discharge caused by improper position of the outlet 114, and improving the efficiency and purity of supernatant purification.

[0071] In one embodiment, P2 = 17cm ~ 19cm. In another embodiment, P2 can be, but is not limited to, any one of 15cm, 17cm, 19cm, 21cm, etc. Thus, this spacing allows for more stable and continuous drainage of the sample solution, further improving the cell purification effect.

[0072] In some embodiments, the cell sedimentation device 100 further includes a fixing member 160 for connecting the second output tube 131 to the inner wall of the second cavity 110b. The fixing member 160 may be, but is not limited to, a membrane, double-sided tape, etc.

[0073] It should be noted that the connection between the fastener 160 and the second output tube 131 can be by adhesive bonding, snap-fitting, etc.

[0074] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0075] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0076] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0077] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0078] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0079] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A cell sedimentation device, characterized in that, The cell sedimentation device includes: A settling bag, wherein the settling bag has a first cavity and a second cavity; the settling bag has a first side and a second side opposite to each other along the height direction of the settling bag, the first cavity is disposed on the first side, the second cavity is disposed on the second side, and the first cavity and the second cavity are connected. The settling bag is further provided with an inlet, an outlet, and a sediment discharge outlet; the inlet is connected to the first cavity and is located close to the first side; the outlet is connected to the second cavity and is located close to the second side; the sediment discharge outlet is connected to the second cavity and is located on the second side, and the sediment discharge outlet and the outlet are spaced apart along the height direction; The inner wall of the second cavity is gradually narrowed in a direction away from the first cavity.

2. The cell sedimentation device according to claim 1, characterized in that, The cell sedimentation device further includes a first output component; the first output component includes a first output tube; the first output tube is connected to at least one of the liquid inlet and the liquid outlet, such that the first output tube is connected to the first cavity and the second cavity.

3. The cell sedimentation device according to claim 2, characterized in that, The first output component further includes a first connecting connector, which has a first end and a second end opposite to each other along its length; the first end is connected to at least one of the liquid inlet and the liquid outlet, and the second end is sealed to the first output tube.

4. The cell sedimentation device according to claim 2, characterized in that, The first output component further includes a first flow regulator, which is disposed in the first output tube and is used to regulate the flow rate of the sample liquid in the first output tube.

5. The cell sedimentation device according to claim 1, characterized in that, The cell sedimentation device further includes a second output component, which includes a second output tube. The second output tube is inserted into and cooperates with the sediment discharge outlet, such that at least a portion of the second output tube is inserted into the second cavity.

6. The cell sedimentation device according to claim 5, characterized in that, The second output pipe has a tapered inlet structure at one end that is inserted into the sediment outlet. The tapered inlet structure is gradually tapered away from the second cavity.

7. The cell sedimentation device according to claim 5, characterized in that, The second output component further includes a second connecting connector, which includes a connector body and a clamping ring; the connector body is provided with a third end and a fourth end opposite to each other along the length direction of the connector body, the third end is sleeved and fitted with the clamping ring, so that the clamping ring is sleeved on the outer side wall of the connector body, and the clamping ring can abut against the side edge of the sedimentation outlet; the fourth end is connected and fitted with the second output pipe.

8. The cell sedimentation device according to claim 5, characterized in that, The second output component further includes a third connector and a third output tube. The third connector has a fifth end and a sixth end, and the extension direction of the fifth end intersects with the extension direction of the sixth end. The fifth end is used to communicate with the second output tube, and the sixth end is used to communicate with the third output tube. The settling bag has a first surface and a second surface opposite to each other along the thickness direction of the settling bag, the liquid outlet is located on the first surface, and the outer wall of the third output pipe is connected and cooperates with the second surface.

9. The cell sedimentation device according to claim 8, characterized in that, The settling bag has a connecting part on the second side, which is used to connect and cooperate with the third output pipe; the orthographic projection area of ​​the connecting part along the thickness direction coincides with the orthographic projection area of ​​the liquid outlet along the thickness direction. And / or, the third output pipe is bonded to the outer surface of the settling bag, such that the outer wall of the third output pipe is bonded to the second surface.

10. A cell purification system, characterized in that, The device includes a sample injection component, a sampling component, a sample receiving component, and the cell sedimentation apparatus according to any one of claims 1 to 9. The sample injection component is configured to communicate with the inlet to inject sample solution into a first cavity through the inlet. The sampling component is configured to communicate with the outlet to inject sample solution into the sampling component from a second cavity. The sample receiving component is configured to communicate with the sedimentation outlet to discharge sample solution from the second cavity to the sample receiving component.

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