Leaf clamping device for chlorophyll fluorescence detection

Abstract

The utility model discloses a leaf clamp device for chlorophyll fluorescence detection, which comprises a leaf chamber, a cavity is arranged in the leaf chamber in a penetrating manner along the vertical direction, and a shading plate capable of transversely moving is mounted at the top of the cavity; a probe is clamped to the top of the leaf chamber, clamping strips are arranged on the left side and the right side of the bottom of the probe respectively, clamping grooves matched with the clamping strips are formed in the top of the leaf chamber, and the probe is located above a leaf chamber cavity; a clamping plate is hinged to the bottom of the blade chamber, a spring is installed between one end of the clamping plate and the blade chamber, a pressing plate is hinged to the other end of the clamping plate, and the pressing plate tightly presses the blade to the opening end of the bottom of the blade chamber. The leaf clamp device for chlorophyll fluorescence detection provided by the utility model can be adapted to leaves with different thicknesses, so that the leaves are uniformly stressed, and the problem of light leakage in the clamping process is avoided; the shading process is simple and easy to operate and high in reliability.

Description

TECHNICAL FIELD

[0001] The utility model relates to a leaf clamp device for chlorophyll fluorescence detection belongs to chlorophyll fluorescence determination technical field. BACKGROUND

[0002] Chlorophyll fluorescence, as a probe for photosynthesis research, has been widely studied and applied. Chlorophyll fluorescence not only reflects the primary reaction process of photosynthesis such as light energy absorption, excitation energy transfer and photochemical reaction, but also is related to electron transfer, proton gradient establishment and ATP synthesis and CO2 fixation processes. Almost all changes in photosynthesis processes can be reflected by chlorophyll fluorescence, and fluorescence measurement technology does not need to break cells and does not harm organisms, so studying chlorophyll fluorescence to indirectly study the changes of photosynthesis is a simple, fast and reliable method.

[0003] Chlorophyll fluorescence detection needs to use a leaf clamp, and the leaf clamp firmly clamps the leaf blade through a mechanical structure to ensure the stability of the leaf blade position during measurement and avoid measurement errors caused by leaf blade movement.

[0004] The leaf clamp in the prior art usually includes upper and lower clamping plates, and the upper and lower clamping plates are connected by a spring or a hinge. When clamping a leaf blade with a large thickness, the upper and lower clamping plates are arranged at a certain angle between them, which easily leads to light leakage problems and affects the measurement accuracy. Some leaf clamps in the prior art have a light shielding function, which can reduce environmental light interference under dark adaptation conditions and improve measurement accuracy. However, the operation of the light shielding process is relatively troublesome, and improper operation can also lead to light leakage problems.

[0005] From the above, it can be seen that the prior art has obvious inconvenience and defects in actual use, so it is necessary to improve. CONTENT OF THE UTILITY MODEL

[0006] In view of the deficiencies in the background art, the utility model provides a leaf clamp device for chlorophyll fluorescence detection, which can adapt to leaf blades of different thicknesses, make the stress on the leaf blades uniform, and avoid light leakage problems during clamping. The operation of the light shielding process is simple and easy to implement, and has high reliability.

[0007] To solve the above technical problems, the utility model adopts the following technical scheme:

[0008] The leaf clamp device for chlorophyll fluorescence detection comprises a leaf chamber, a cavity is arranged through the vertical direction inside the leaf chamber, a light shielding plate that can move horizontally is installed at the top of the cavity, a probe is clamped at the top of the leaf chamber, the probe is located above the cavity of the leaf chamber, a clamping plate is hingedly connected to the bottom of the leaf chamber, a spring is installed between one end of the clamping plate and the leaf chamber, a pressing plate is hingedly connected to the other end of the clamping plate, and the pressing plate presses the leaf blade tightly on the bottom opening end of the leaf chamber.

[0009] Further, the left and right sides of the bottom of the probe are respectively provided with clamping strips.

[0010] Further, the top of the leaf chamber is provided with a clamping groove matched with the clamping strip.

[0011] Further, the leaf chamber is provided with a limiting plate integrally formed thereon, and the limiting plate is located outside the light shielding plate.

[0012] Further, the top end of the spring is sleeved on the positioning column A, and the bottom end of the spring is sleeved on the positioning column B.

[0013] Further, the positioning column A is located at the side of the leaf chamber close to the clamping plate, and the positioning column B is located at the side of the clamping plate close to the leaf chamber.

[0014] Further, the bottom of the leaf chamber is connected with the clamping plate through the rotating shaft A, and the clamping plate can swing at the rotating shaft A.

[0015] Further, the pressing plate is connected with the clamping plate through the rotating shaft B, and the pressing plate can swing at the rotating shaft B.

[0016] After the above technical scheme is adopted, compared with the prior art, the utility model has the following advantages:

[0017] The clamping plate drives the pressing plate to swing, the blade is pressed on the bottom opening end of the leaf chamber through the pressing plate, the pressing plate can swing at the rotating shaft B, so that the blade is parallelly pressed on the bottom opening end of the leaf chamber, no matter the thickness of the blade, the blade can be parallelly stressed, and the problem of light leakage due to uneven stress is avoided; the chamber can be closed or opened by pulling the light shielding plate, so that the blade is shaded, dark adaptation is carried out, and the shading process is simple and easy to operate and has high reliability.

[0018] The utility model will be described in detail below in combination with the drawings and examples. DRAWINGS

[0019] Figure 1 is the structure front view of the utility model;

[0020] Figure 2 is the structure perspective view of the utility model;

[0021] Figure 3 is the structure perspective view of the utility model along another direction;

[0022] Figure 4 is the cross section schematic view of the utility model;

[0023] Figure 5 is the structure perspective view of the leaf chamber.

[0024] In the diagram, 1-probe, 11-jaw strip; 2-leaf chamber, 21-jaw groove, 22-limiting plate, 23-positioning post A; 3-clamping plate, 31-positioning post B; 4-pressure plate; 5-light shield; 6-rotating shaft A; 7-rotating shaft B; 8-spring. Detailed Implementation

[0025] To provide a clearer understanding of the technical features, objectives, and effects of this utility model, the specific embodiments of this utility model are now described with reference to the accompanying drawings.

[0026] like Figures 1-5 As shown in the figure, this utility model provides a leaf clamp device for chlorophyll fluorescence detection, including a leaf chamber 2, inside which there is a cavity that runs vertically through the leaf chamber 2, and a light-shielding plate 5 that can move laterally is installed on the top of the cavity; a probe 1 is snapped onto the top of the leaf chamber 2, and the probe 1 is located above the cavity of the leaf chamber 2; a clamping plate 3 is hinged to the bottom of the leaf chamber 2, a spring 8 is installed between one end of the clamping plate 3 and the leaf chamber 2, and a pressure plate 4 is hinged to the other end of the clamping plate 3, and the pressure plate 4 presses the leaf tightly against the bottom opening end of the leaf chamber 2.

[0027] The probe 1 has a retaining strip 11 on the left and right sides of its bottom; the top of the blade chamber 2 has a retaining groove 21 that matches the retaining strip 11. The retaining strip 11 of the probe 1 can slide from the side of the blade chamber 2 into the retaining groove 21 to achieve convenient assembly of the probe 1 and the blade chamber 2.

[0028] The leaf chamber 2 is provided with an integrally formed limiting plate 22. The limiting plate 22 is located outside the light-shielding plate 5 and is used to limit the sliding stroke of the light-shielding plate 5 to prevent the light-shielding plate 5 from falling off during the sliding process. The light-shielding plate 5 is used to block light and can close or open the chamber of the leaf chamber 2 to block light from the blade and perform dark adaptation.

[0029] The top end of the spring 8 is fitted onto the positioning post A23, which is located on the side of the blade chamber 2 near the clamping plate 3. The bottom end of the spring 8 is fitted onto the positioning post B31, which is also located on the side of the clamping plate 3 near the blade chamber 2. The cooperation between the positioning posts A23 and B31 prevents the spring 8 from falling off during use.

[0030] The bottom of the leaf chamber 2 is connected to the clamping plate 3 via a rotating shaft A6, and the clamping plate 3 can swing along the rotating shaft A6.

[0031] The pressure plate 4 is connected to the clamping plate 3 via the rotating shaft B7. The pressure plate 4 can swing along the rotating shaft B7, which can press the blade parallel to the bottom opening of the blade chamber 2. Regardless of the thickness of the blade, it can be subjected to parallel force.

[0032] The probe 1 is equipped with components such as a light source and a light sensor, which can directly trigger and detect fluorescence signals.

[0033] The specific working principle of this utility model is as follows:

[0034] In use, pressing the clamp 3 causes the spring 8 to contract, and the clamp 3 drives the pressure plate 4 to swing. After releasing the clamp 3, the spring 8 returns to its original position, pressing the blade against the bottom opening of the blade chamber 2 through the pressure plate 4. The pressure plate 4 can swing along the pivot B7, thus pressing the blade parallel to the bottom opening of the blade chamber 2. Regardless of the thickness of the blade, it can be subjected to parallel force, avoiding light leakage due to uneven force. The chamber of the blade chamber 2 can be closed or opened by pulling the light shield 5 to shield the blade and perform dark adaptation. The fluorescence signal is triggered and detected by the probe 1.

[0035] The above description provides examples of the preferred embodiments of this utility model. Any aspects not detailed herein are common knowledge to those skilled in the art. The scope of protection of this utility model is determined by the claims. Any equivalent modifications based on the technical teachings of this utility model are also within the scope of protection of this utility model.

Claims

1. A leaf clip device for chlorophyll fluorescence detection, characterized in that: Includes a blade chamber (2), inside which is a chamber arranged vertically through the blade, and a light-shielding plate (5) that can move laterally is installed on the top of the chamber; a probe (1) is snapped onto the top of the blade chamber (2), and the probe (1) is located above the chamber of the blade chamber (2); a clamping plate (3) is hinged to the bottom of the blade chamber (2), and a spring (8) is installed between one end of the clamping plate (3) and the blade chamber (2), and a pressure plate (4) is hinged to the other end of the clamping plate (3), and the pressure plate (4) presses the blade tightly onto the bottom opening end of the blade chamber (2).

2. The leaf clip device for chlorophyll fluorescence detection as described in claim 1, characterized in that: The probe (1) has locking strips (11) on the left and right sides of its bottom.

3. The leaf clip device for chlorophyll fluorescence detection as described in claim 2, characterized in that: The top of the leaf chamber (2) is provided with a slot (21) that is compatible with the card strip (11).

4. The leaf clip device for chlorophyll fluorescence detection as described in claim 1, characterized in that: The leaf chamber (2) is provided with a limiting plate (22) integrally formed therewith, and the limiting plate (22) is located outside the light shield (5).

5. The leaf clip device for chlorophyll fluorescence detection as described in claim 1, characterized in that: The top end of the spring (8) is fitted onto the positioning post A (23), and the bottom end of the spring (8) is fitted onto the positioning post B (31).

6. The leaf clip device for chlorophyll fluorescence detection as described in claim 5, characterized in that: Positioning post A (23) is located on the side of the blade chamber (2) near the clamping plate (3), and positioning post B (31) is located on the side of the clamping plate (3) near the blade chamber (2).

7. The leaf clip device for chlorophyll fluorescence detection as described in claim 1, characterized in that: The bottom of the leaf chamber (2) is connected to the clamping plate (3) via a rotating shaft A (6), and the clamping plate (3) can swing along the rotating shaft A (6).

8. The leaf clip device for chlorophyll fluorescence detection as described in claim 7, characterized in that: The pressure plate (4) is connected to the clamping plate (3) via the rotating shaft B (7), and the pressure plate (4) can swing along the rotating shaft B (7).

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