Two-channel exosome extraction device

Abstract

The utility model discloses a double-channel exosome extraction device, which belongs to the field of exosome extraction and comprises an ultrasonic cleaning container, two ultrasonic suction filtration modules, a pipeline, a regulating valve, a pressure gauge, an electromagnetic valve and an external ultrasonic vibrator. The input ends of the ultrasonic suction filtration modules are fixedly connected with pipelines, the input ends of the pipelines are fixedly connected with adjusting valves, pressure gauges are arranged at the tops of the adjusting valves, the input ends of the two adjusting valves are fixedly connected with electromagnetic valves, and an external ultrasonic vibrator is arranged in the ultrasonic cleaning container. Through cooperation of external ultrasonic cleaning and internal ultrasonic cleaning of ultrasonic suction filtration, the ultrafiltration membrane is prevented from being blocked in the exosome extraction process, and the extraction efficiency is greatly improved. And the ultrasonic power on the suction filtration module of the device can be adjusted, so that dynamic adjustment is realized according to the blockage condition, and the condition that a sample and an ultrafiltration membrane are damaged by temperature rise due to long-time opening is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of exosome extraction, in particular to a double-channel exosome extraction device. BACKGROUND

[0002] Exosomes are nanoscale vesicles secreted by cells, carrying biological molecules such as proteins and RNA, and participating in cell-to-cell communication and disease regulation. Exosome extraction devices are tools for isolating exosomes from biological samples. Common methods include ultracentrifugation, filtration, and kit extraction. Their functions include disease diagnostic markers, drug delivery carriers, and cell-to-cell signal transmission research.

[0003] Exosome negative pressure filtration extraction devices typically include a filter membrane, a negative pressure system, a sample loading chamber, and a collection chamber. The working principle is based on negative pressure driving and size exclusion: the sample passes through the filter membrane with a specific pore size under the action of negative pressure, and large particles and cell fragments are trapped, while exosomes and small molecules pass through the membrane pores into the collection chamber, thereby achieving efficient separation and extraction of exosomes.

[0004] In existing filtration extraction technology, a large amount of material larger than the filter membrane pore size adheres to the ultrafiltration membrane during the negative pressure filtration process, blocking the filter membrane, thereby continuously reducing the filtration efficiency and reducing the yield of exosomes. In addition, increasing the negative pressure during the period may cause the filter membrane to be damaged, and reducing the negative pressure will reduce the extraction efficiency. Therefore, how to avoid filter membrane blockage and ensure extraction efficiency is a key technology.

[0005] Based on this, the utility model discloses a double-channel exosome extraction device to solve the above problems. Utility model content

[0006] In view of the above shortcomings existing in the prior art, the utility model provides a double-channel exosome extraction device.

[0007] To achieve the above purpose, the utility model realizes through the following technical scheme:

[0008] The double-channel exosome extraction device comprises an ultrasonic cleaning container, and further comprises an ultrasonic filtration module, a pipeline, an adjusting valve, a pressure gauge, an electromagnetic valve and an external ultrasonic vibrator. The inside of the ultrasonic cleaning container is fixedly connected with two ultrasonic filtration modules that can be used alternately. The input end of the ultrasonic filtration module is fixedly connected with the pipeline. The input end of the pipeline is fixedly connected with the adjusting valve. The top of the adjusting valve is provided with the pressure gauge. The input ends of the two adjusting valves are fixedly connected with the electromagnetic valve. The inside of the ultrasonic cleaning container is provided with the external ultrasonic vibrator.

[0009] The ultrasonic filtration module comprises an upper shell, a lower shell, a quick release assembly, a negative pressure gas source quick connector, a fixing assembly, an ultrafiltration membrane and an ultra-thin gasket, the fixing assembly is fixedly connected to the outside of the upper shell and the lower shell, the negative pressure gas source quick connector is fixedly connected to the top of the upper shell, the input end of the negative pressure gas source quick connector is fixedly connected to the output end of the pipeline, the fixing assembly and the ultrafiltration membrane are sequentially stacked in the lower shell from top to bottom, the ultrafiltration membrane is provided with the ultra-thin gasket on the upper side and the lower side, and the ultrafiltration membrane is fixed through the butt joint of the upper shell and the lower shell;

[0010] Further, the inner bottom of the upper shell is electrically connected with an internal ultrasonic vibrator, and the bottom of the internal ultrasonic vibrator is located above the ultrafiltration membrane and synchronously works with the external ultrasonic vibrator;

[0011] Further, the quick release assembly comprises four buckles, the buckles are divided into two parts, the proximal end of the upper half of the buckle is fixedly connected to the outside of the upper shell, the proximal end of the butt joint block of the lower half of the buckle is fixedly connected to the outside of the lower shell, and the bottom of the upper shell is detachably connected to the top of the lower shell through the buckle;

[0012] Further, the fixing assembly comprises a silica gel gasket and a support disc, the silica gel gasket is located above the support disc and has a thickness of 2 mm, and is used for preventing leakage;

[0013] Further, the lower side of the ultrafiltration membrane is not shielded, so as to increase the working area of the ultrafiltration membrane, facilitate cleaning and maintenance, and the distance from the ultrafiltration membrane to the bottom of the external ultrasonic vibrator is 2 mm, so as to improve the concentration of exosomes after filtration;

[0014] Further, the internal bottom of the upper shell is fixedly connected with an ultrasonic vibrator hardware circuit, the bottom of the ultrasonic vibrator hardware circuit is electrically connected with the internal ultrasonic vibrator and the external ultrasonic vibrator, the top of the upper shell is provided with a pneumatic quick connector mounting hole, the top of the upper shell is provided with an ultrasonic vibrator power supply interface, and the outside of the upper shell is provided with a buckle mounting hole;

[0015] Further, the bottom of the lower shell is provided with a spiral flow channel, which is used for continuously flushing the surface of the ultrafiltration membrane in the tangential direction, so as to prevent the ultrafiltration membrane from being blocked;

[0016] Further, a plurality of waste liquid holes are formed in the inside of the support disc, a plurality of flow channels are formed in the inside of the support disc, the waste liquid holes are connected with the flow channels, and the bottom of the support disc is fixedly connected with a plurality of filter membrane support convex points.

[0017] The utility model discloses a compared with prior art, its beneficial effect is: 1, through the ultrasonic transducer hardware control circuit control ultrasonic filtration module's inside ultrasonic transducer and external ultrasonic transducer work, the inside and outside of filter membrane are cleaned simultaneously, and the ultrafiltration membrane permeation flux is strengthened, thereby the clogging adhered to the filter membrane because of the filtration negative pressure effect is washed down, realizes the unobstructed of ultrafiltration membrane, and the power of the ultrasonic vibration source on the filtration module of the device can be adjusted to avoid the warming damage of the continuous work sample to the exosome of ultrasonic,

[0018] 2, through the alternate closing solenoid valve, let the negative pressure act on different ultrasonic filtration module, switch two groups of ultrasonic filtration module alternate work, when one group of ultrafiltration membrane is in the clean state, another group of ultrafiltration module starts working, and the ultrafiltration module that stops working removes the clogging under the action of ultrasonic more efficiently and completely, realizes the continuous and efficient exosome purification work. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical scheme in the embodiment of the utility model or prior art, the following will be briefly introduced the drawing needed to be used in the embodiment or prior art description. Obviously, the drawing in the following description is only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to these drawings without creating labor.

[0020] Figure 1 It is a perspective view of a double-channel exosome extraction device of the utility model;

[0021] Figure 2 It is a front view of a double-channel exosome extraction device of the utility model;

[0022] Figure 3 It is the structure explosion drawing of the ultrasonic filtration module of a double-channel exosome extraction device of the utility model;

[0023] Figure 4 It is the structure schematic view of the supporting disc of a double-channel exosome extraction device of the utility model;

[0024] Figure 5 It is the structure schematic view of the upper shell of a double-channel exosome extraction device of the utility model;

[0025] Figure 6 It is the structure schematic view of the lower shell of a double-channel exosome extraction device of the utility model;

[0026] Figure 7 It is the structure schematic view of the lock catch of a double-channel exosome extraction device of the utility model.

[0027] The reference numerals in the drawing represent respectively:

[0028] 1, ultrasonic cleaning vessel; 2, ultrasonic filtration module; 21, upper shell; 211, pneumatic quick connector mounting hole; 212, ultrasonic transducer power interface; 213, lock catch mounting hole; 214, ultrasonic transducer hardware circuit; 22, lower shell; 221, spiral flow channel; 23, lock catch; 24, negative pressure air source quick connector; 25, internal ultrasonic transducer; 26, silica gel gasket; 27, support disc; 271, filter membrane support protrusion; 272, flow channel; 273, waste liquid hole; 28, ultra-thin gasket; 29, ultrafiltration membrane; 3, pipeline; 4, regulating valve; 5, pressure gauge; 6, electromagnetic valve; 7, external ultrasonic transducer. DETAILED DESCRIPTION

[0029] To make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described below in connection with the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0030] In the following description, "left", "right", "front", "back", "up", "down" are oriented in the perspective of the front view.

[0031] Embodiment one: in some embodiments, referring to the accompanying drawings of the specification Figures 1-7 , the double-channel exosome extraction device comprises an ultrasonic cleaning vessel 1, further comprising an ultrasonic filtration module 2, a pipeline 3, a regulating valve 4, a pressure gauge 5, an electromagnetic valve 6 and an external ultrasonic transducer 7, the inside of the ultrasonic cleaning vessel 1 is fixedly connected with two ultrasonic filtration modules 2 which can be used alternately, the input end of the ultrasonic filtration module 2 is fixedly connected with the pipeline 3, the input end of the pipeline 3 is fixedly connected with the regulating valve 4, the top of the regulating valve 4 is provided with the pressure gauge 5, and the input ends of the two regulating valves 4 are fixedly connected with the electromagnetic valve 6.

[0032] The top of the ultrasonic cleaning container 1 is provided with a mixed solution to be extracted, and the electromagnetic valve 6 is responsible for outputting the input negative pressure to the ultrasonic filtration module 2, thereby controlling the working state of the ultrasonic filtration module 2; when the electromagnetic valve 6 is opened, the ultrasonic filtration module 2 operates to filter and purify the exosome solution in the container, so that the non-exosome substances (water, free proteins and small particles) in the sample solution are extracted into the pipeline 3, and the purified exosome solution remains in the container; when the electromagnetic valve 6 is closed, the ultrasonic filtration module 2 stops filtration, and the device adopts two groups of ultrasonic filtration modules 2; when one group of ultrafiltration membranes 29 is in a clean state, the other group of ultrafiltration modules starts to work, realizing continuous and efficient exosome purification work.

[0033] The ultrasonic filtration module 2 comprises an upper shell 21, a lower shell 22, a quick-release assembly, a negative pressure gas source quick connector 24, a fixing assembly, an ultrafiltration membrane 29 and an ultra-thin gasket 28; the outer part of the upper shell 21 and the lower shell 22 is fixedly connected with the fixing assembly; the top of the upper shell 21 is fixedly connected with the negative pressure gas source quick connector 24, and the input end of the negative pressure gas source quick connector 24 is fixedly connected with the output end of the pipeline 3; the inside of the lower shell 22 is sequentially stacked from top to bottom with the fixing assembly and the ultrafiltration membrane 29, and the upper and lower sides of the ultrafiltration membrane 29 are provided with the ultra-thin gasket 28, and the ultrafiltration membrane 29 is fixed by the butt joint of the upper shell 21 and the lower shell 22.

[0034] The quick-release assembly comprises four lock buckles 23, which are divided into two parts; the proximal end of the upper half of the buckle ring of the lock buckle 23 is fixedly connected to the outer part of the upper shell 21, and the proximal end of the butt block of the lower half of the lock buckle 23 is fixedly connected to the outer part of the lower shell 22; the bottom of the upper shell 21 is detachably connected to the top of the lower shell 22 through the lock buckle 23.

[0035] The inner bottom of the upper shell 21 is electrically connected with an internal ultrasonic vibrator 25, and the inside of the ultrasonic cleaning container 1 is provided with an external ultrasonic vibrator 7; the bottom of the internal ultrasonic vibrator 25 is above the ultrafiltration membrane 29 and works synchronously with the external ultrasonic vibrator 7.

[0036] The internal ultrasonic vibrator 25 and the external ultrasonic vibrator 7 work to clean the inside and outside of the ultrafiltration membrane 29 at the same time, strengthen the permeation flux of the ultrafiltration membrane 29, thereby washing away the blockages adhered to the ultrafiltration membrane 29 due to the effect of filtration negative pressure, and realizing the smoothness of the ultrafiltration membrane 29.

[0037] The fixing assembly comprises a silica gel gasket 26 and a supporting disc 27; the silica gel gasket 26 is above the supporting disc 27, and the thickness of the silica gel gasket 26 is 2mm, which is used for leakage prevention.

[0038] The lower part of the ultrafiltration membrane 29 is not shielded, so as to increase the working area of the ultrafiltration membrane 29, facilitate cleaning and maintenance, and the distance from the ultrafiltration membrane 29 to the bottom of the external ultrasonic vibrator 7 is 2mm, so as to improve the concentration of the filtered exosomes.

[0039] The inside of the upper shell 21 is fixedly connected with an ultrasonic transducer hardware circuit 214, and the bottom of the ultrasonic transducer hardware circuit 214 is electrically connected with an internal ultrasonic transducer 25 and an external ultrasonic transducer 7. The top of the upper shell 21 is provided with a pneumatic quick connector mounting hole 211. The top of the upper shell 21 is provided with an ultrasonic transducer power supply interface 212. The outside of the upper shell 21 is provided with a lock catch mounting hole 213.

[0040] The bottom of the lower shell 22 is provided with a spiral flow channel 221 for continuously flushing the surface of the ultrafiltration membrane 29 in a tangential flow to prevent the ultrafiltration membrane 29 from being blocked.

[0041] When the ultrasonic filtration module 2 is working, the pipeline connected with the upper shell 21 provides negative pressure, so that the sample solution flows upward through the ultrafiltration membrane 29 in the inside of the lower shell 22. The flow channel 272 below the lower shell 22 uses a spiral flow channel 272 to continuously flush the surface of the ultrafiltration membrane 29 in a tangential flow to prevent the ultrafiltration membrane 29 from being blocked. Leakage is prevented by a 2mm silica gel gasket 26, so that the non-exosome substances in the sample solution seep upward into the upper layer of the ultrafiltration membrane 29. The internal ultrasonic transducer 25 is vibrated by the internal ultrasonic transducer 25 hardware control circuit. Since the low-power ultrasonic wave is less than 20W, the damage to the filter membrane is avoided, the low-power ultrasonic wave strengthens the permeation flux of the ultrafiltration membrane 29, the extracted material adhered to the lower part of the filter membrane is washed down, the reuse of the ultrafiltration membrane 29 is realized, the power of the ultrasonic vibration source on the filtration module of the device can be adjusted, and the sample is prevented from being damaged by sample warming during continuous ultrasonic work.

[0042] The inside of the support disc 27 is provided with a plurality of waste liquid holes 273 and a plurality of flow channels 272. The waste liquid holes 273 are connected with the flow channels 272. The bottom of the support disc 27 is fixedly connected with a plurality of filter membrane support protrusions 271.

[0043] The support disc 27 is uniformly distributed with a plurality of waste liquid holes 273 within a 360-degree angle. The waste liquid holes 273 are connected with the flow channels 272 with a depth of 0.5mm. The pressure on both sides of the ultrafiltration membrane 29 is uniformly distributed, so as to increase the working area of the ultrafiltration membrane 29 and prevent the blockage of the ultrafiltration membrane 29 caused by the accumulation of impurities. The dense filter membrane support protrusions 271 provide dispersed and effective support for the ultrafiltration membrane 29 to prevent the ultrafiltration membrane 29 from being damaged under negative pressure.

[0044] In the overall structure, the double-ultrasonic filtration module is stacked with an external ultrasonic design. The double-ultrasonic filtration module realizes an alternating sampling extraction process and avoids the situation that the cleaning efficiency of the filter is not high during continuous work. One of the modules is always in the best working state. The internal ultrasonic wave is responsible for internal cleaning of the filter membrane, and the external ultrasonic wave is responsible for external cleaning of the filter membrane. The long-term effectiveness of the filter is ensured.

[0045] The above embodiments are only used to illustrate the technical solutions of the present application, and are not intended to limit the present application; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features therein can be replaced by equivalents; and these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A dual-channel exosome extraction device, comprising an ultrasonic cleaning container (1), characterized in that: It also includes an ultrasonic filtration module (2), a pipe (3), a regulating valve (4), a pressure gauge (5), a solenoid valve (6), and an external ultrasonic transducer (7). The ultrasonic cleaning container (1) is internally connected to two ultrasonic filtration modules (2) that can be used alternately. The input end of the ultrasonic filtration module (2) is fixedly connected to a pipe (3). The input end of the pipe (3) is fixedly connected to a regulating valve (4). A pressure gauge (5) is installed on the top of the regulating valve (4). The input ends of the two regulating valves (4) are fixedly connected to a solenoid valve (6). The ultrasonic cleaning container (1) is internally equipped with an external ultrasonic transducer (7). The ultrasonic filtration module (2) includes an upper shell (21), a lower shell (22), a quick-release assembly, a negative pressure air source quick connector (24), a fixing assembly, an ultrafiltration membrane (29), and an ultrathin gasket (28). The upper shell (21) and the lower shell (22) are fixedly connected to the outside of the fixing assembly. The top of the upper shell (21) is fixedly connected to the negative pressure air source quick connector (24). The input end of the negative pressure air source quick connector (24) is fixedly connected to the output end of the pipe (3). The fixing assembly and the ultrafiltration membrane (29) are stacked from top to bottom inside the lower shell (22). Ultrathin gaskets (28) are provided on both the upper and lower sides of the ultrafiltration membrane (29). The ultrafiltration membrane (29) is fixed by the docking of the upper shell (21) and the lower shell (22).

2. The dual-channel exosome extraction device according to claim 1, characterized in that, The inner bottom of the upper shell (21) is electrically connected to an internal ultrasonic transducer (25), and the bottom of the internal ultrasonic transducer (25) is located above the ultrafiltration membrane (29) and works synchronously with the external ultrasonic transducer (7).

3. The dual-channel exosome extraction device according to claim 1, characterized in that, The quick-release assembly includes four latches (23). The latches (23) are divided into two parts. The upper part of the latch (23) is fixedly connected to the outside of the upper shell (21) at one end of the buckle, and the lower part of the latch (23) is fixedly connected to the outside of the lower shell (22) at one end of the mating block. The bottom of the upper shell (21) is detachably connected to the top of the lower shell (22) through the latches (23).

4. The dual-channel exosome extraction device according to claim 1, characterized in that, The fixing components include a silicone gasket (26) and a support disk (27). The silicone gasket (26) is located above the support disk (27) and has a thickness of 2 mm for leak prevention.

5. The dual-channel exosome extraction device according to claim 2, characterized in that, The underside of the ultrafiltration membrane (29) is unobstructed to increase the working area of ​​the ultrafiltration membrane (29) and facilitate cleaning and maintenance. The distance from the ultrafiltration membrane (29) to the bottom of the external ultrasonic transducer (7) is 2 mm, thereby increasing the concentration of exosomes after filtration.

6. The dual-channel exosome extraction device according to claim 2, characterized in that, The upper shell (21) is internally fixedly connected to an ultrasonic transducer hardware circuit (214). The bottom of the ultrasonic transducer hardware circuit (214) is electrically connected to an internal ultrasonic transducer (25) and an external ultrasonic transducer (7). The top of the upper shell (21) is provided with a pneumatic quick connector mounting hole (211). The top of the upper shell (21) is provided with an ultrasonic transducer power interface (212). The outside of the upper shell (21) is provided with a latch mounting hole (213).

7. The dual-channel exosome extraction device according to claim 1, characterized in that, The bottom of the lower shell (22) is provided with a spiral flow channel (221) for continuously tangentially scouring the surface of the ultrafiltration membrane (29) to prevent the ultrafiltration membrane (29) from clogging.

8. The dual-channel exosome extraction device according to claim 4, characterized in that, The support disc (27) has multiple waste liquid holes (273) inside and multiple flow channels (272) inside. The waste liquid holes (273) are connected to the flow channels (272). Multiple filter membrane support protrusions (271) are fixedly connected to the bottom of the support disc (27).

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