Reaction cavity structure and micro-fluidic chip
By setting a cavity around the reaction chamber of the microfluidic chip and using a thin sealing layer, the problems of uneven temperature distribution and heat loss were solved, and a more efficient amplification reaction was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU BAOCHUANG BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-05-15
- Publication Date
- 2026-04-24
AI Technical Summary
In existing microfluidic chips, the temperature distribution in the reaction chamber is uneven and heat is easily conducted to the outside, resulting in a decrease in amplification reaction efficiency.
A reaction chamber structure is designed with an outer cavity to block temperature conduction and a thin sealing layer to quickly transfer heat, ensuring temperature uniformity and insulation within the reaction chamber.
This improved the efficiency of the amplification reaction, ensured the uniformity of temperature within the reaction chamber, reduced heat loss, and thus enhanced the efficiency of the amplification reaction.
Smart Images
Figure CN224156887U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of microfluidics, and in particular to a reaction chamber structure and a microfluidic chip. Background Technology
[0002] In related technologies, microfluidic chips, also known as lab-on-a-chip, offer advantages such as small sample volumes, rapid analysis speed, ease of fabrication into portable instruments, and suitability for point-of-care and on-site analysis. They have been widely applied in numerous fields, including biology, chemistry, and medicine. The combination of microfluidic chips with in vitro diagnostic reagents and technologies has led to the development of many rapid detection techniques, such as microfluidic-based colloidal gold immunoassay, immunofluorescence assay, chemiluminescence immunoassay, and microfluidic-based nucleic acid assay.
[0003] During amplification, the reaction chamber of the microfluidic chip needs to be heated to maintain the optimal reaction temperature. However, in current microfluidic chips, the temperature distribution within the reaction chamber is uneven, and heat is easily conducted to the outside, making it difficult to maintain temperature stability and reducing the efficiency of the amplification reaction. Utility Model Content
[0004] This application aims to address at least one of the technical problems existing in the prior art. To this end, this application proposes a reaction chamber structure and a microfluidic chip that can effectively improve the efficiency of amplification reactions.
[0005] An embodiment of the first aspect of this application provides a reaction chamber structure, including:
[0006] A substrate is provided with a reaction chamber and a cavity. The substrate has a first end face and a second end face disposed opposite to each other. The reaction chamber is disposed through the first end face and the second end face. At least one cavity is disposed on the outer periphery of the reaction chamber.
[0007] A first sealing layer and a second sealing layer, wherein the first sealing layer covers the first end face and the second sealing layer covers the second end face.
[0008] According to the reaction chamber structure of the first aspect of this application, the reaction chamber is flat.
[0009] According to the reaction chamber structure of the first aspect of this application, the cavity is arc-shaped.
[0010] According to the reaction chamber structure of the first aspect of this application, the cavity is disposed through the first end face and the second end face.
[0011] According to the reaction chamber structure of the first aspect of this application, four cavities are arranged at intervals around the outer periphery of the reaction chamber.
[0012] According to the reaction chamber structure of the first aspect embodiment of this application, the reaction chamber has a liquid inlet end and a liquid outlet end, the substrate is provided with a first flow channel and a second flow channel, one end of the first flow channel is in fluid communication with the liquid inlet end, one end of the second flow channel is in communication with the liquid outlet end, the first flow channel is located between one set of adjacent cavities, and the second flow channel is located between the other two cavities.
[0013] According to the reaction chamber structure of the first aspect of this application, the reaction chamber is symmetrically arranged about the connecting line between the liquid inlet end and the liquid outlet end.
[0014] According to the reaction chamber structure of the first aspect of this application, the number of reaction chambers is at least one, and at least one cavity is provided on the outer periphery of each reaction chamber.
[0015] According to the reaction chamber structure of the first aspect of this application, the outline of the reaction chamber is hexagonal.
[0016] An embodiment of the second aspect of this application provides a microfluidic chip, including the reaction chamber structure as described above.
[0017] As can be seen from the above technical solutions, the embodiments of this application have at least the following beneficial effects:
[0018] In the reaction chamber structure and microfluidic chip provided in this application embodiment, a cavity is provided on the outer periphery of the reaction chamber. When the amplification reagent undergoes an amplification reaction within the reaction chamber, the cavity can block the heat conduction from the reaction chamber to the outside, which is beneficial to improving the efficiency of the amplification reaction within the reaction chamber. Simultaneously, the reaction chamber is disposed through the first and second end faces of the substrate, and the thickness of the first and second sealing layers is relatively small, allowing heat to be rapidly transferred to the reaction chamber and ensuring that the temperature at various locations within the reaction chamber remains consistent. This also helps to reduce heat loss within the reaction chamber, thereby guaranteeing the efficiency of the amplification reaction. Attached Figure Description
[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0020] Figure 1 This is a schematic diagram of the reaction chamber structure provided in one embodiment of this application;
[0021] Figure 2 This is a schematic diagram of the substrate structure in one embodiment of this application;
[0022] Figure 3 for Figure 2 A schematic diagram of the structure on the back side of the substrate;
[0023] Figure 4 This is a schematic diagram of the structure of a microfluidic chip provided in one embodiment of this application.
[0024] Figure label:
[0025] 100, substrate; 110, reaction chamber; 120, cavity; 130, first flow channel; 140, second flow channel;
[0026] 200. First sealing layer;
[0027] 300. Second sealing layer. Detailed Implementation
[0028] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0029] See Figure 1 As shown, an embodiment of the first aspect of this application discloses a reaction chamber structure, including a substrate 100 and a sealing assembly.
[0030] Specifically, the substrate 100 has a first end face and a second end face disposed opposite to each other. The substrate 100 is provided with a reaction chamber 110 and a cavity 120. The reaction chamber 110 is disposed through the first end face and the second end face. At least one cavity 120 is disposed on the outer periphery of the reaction chamber 110. The sealing assembly includes a first sealing layer 200 and a second sealing layer 300. The first sealing layer 200 covers the first end face, and the second sealing layer 300 covers the second end face.
[0031] In practical applications, the reaction chamber 110 is equipped with amplification reagents. When the elution product enters the reaction chamber 110, the amplification reaction takes place within the reaction chamber 110.
[0032] Furthermore, during the amplification reaction, the reaction chamber 110 is equipped with a heating area of a heating device. By heating the solution in the reaction chamber 110 through the heating device, the temperature of the amplification reaction can be increased, thereby improving the amplification reaction efficiency.
[0033] In the reaction chamber structure provided in this embodiment, a cavity 120 is provided on the outer periphery of the reaction chamber 110. When the amplification reagent undergoes an amplification reaction within the reaction chamber 110, the cavity 120 can block the heat conduction from the reaction chamber 110 to the outside, thereby improving the efficiency of the amplification reaction within the reaction chamber 110. Simultaneously, the reaction chamber 110 is disposed through the first and second end faces of the substrate 100, and the thicknesses of the first sealing layer 200 and the second sealing layer 300 are relatively small, allowing heat to be quickly transferred to the reaction chamber 110 and reducing heat loss within the reaction chamber 110, thus ensuring the efficiency of the amplification reaction.
[0034] In this embodiment, see Figures 1 to 3 As shown, the substrate 100 has a first end face and a second end face disposed opposite to each other. The reaction chamber 110 is disposed through the first end face and the second end face. A first sealing layer 200 covers the first end face, and a second sealing layer 300 covers the second end face. The first sealing layer 200 and the second sealing layer 300 are made of a material with a small thickness (such as a film material), which can quickly conduct heat. This is beneficial for improving the amplification reaction efficiency.
[0035] It is understandable that the first sealing layer 200 and the second sealing layer 300 should be made of materials with good thermal conductivity.
[0036] In some embodiments of this application, the reaction chamber 110 is flat. This allows the liquid within the reaction chamber 110 to heat up rapidly and also helps to ensure a uniform temperature distribution throughout the chamber, thereby guaranteeing amplification efficiency.
[0037] In practical applications, the thickness of the substrate 100 can be set to 0.1~2mm as needed. For example, the thickness of the substrate 100 can be set to 0.5mm, so that the reaction chamber 110 is flat, thereby improving the heat transfer rate and ensuring the amplification reaction efficiency.
[0038] In some embodiments of this application, see Figures 1 to 3 The cavity 120 is arc-shaped. The arc-shaped cavity 120 surrounds the outer periphery of the reaction chamber 110. The cavity 120 isolates the reaction chamber 110 from the outside, thereby reducing heat loss from the reaction chamber 110, ensuring the reaction temperature of the reaction chamber 110, and thus facilitating the amplification reaction efficiency.
[0039] It is understandable that the specific curvature of cavity 120 can be adaptively adjusted according to the outer contour of reaction chamber 110, and is not limited here.
[0040] Further, see Figure 2 and Figure 3The cavity 120 is provided through the first end face and the second end face. This reduces heat transfer at the cavity 120 location, which helps to reduce heat loss from the reaction chamber 110 and further ensures the reaction efficiency of the amplification reaction.
[0041] In some embodiments of this application, see Figure 2 and Figure 3 As shown, four cavities 120 are arranged at intervals around the outer periphery of the reaction chamber 110. The four cavities 120 are arranged sequentially at intervals around the outer periphery of the reaction chamber 110. In this way, the heat preservation effect of the reaction chamber 110 can be further improved, the heat loss of the reaction chamber 110 can be reduced, and the efficiency of the amplification reaction can be improved.
[0042] It is understandable that the number of cavities 120 around the reaction chamber 110 can be set according to the needs of heat insulation and layout. For example, the number of cavities 120 can be set to 3, 5, etc.
[0043] In the embodiments of this application, the substrate 100 is provided with a first flow channel 130 and a second flow channel 140, and the reaction chamber 110 has a liquid inlet end and a liquid outlet end. The first flow channel 130, which is connected to the liquid inlet end, is located between two adjacent cavities 120; similarly, the second flow channel 140, which is connected to the liquid outlet end, is located between another pair of adjacent cavities 120. That is to say, the cavities 120 in the substrate 100 are arranged to avoid the first flow channel 130 and the second flow channel 140, so that the first flow channel 130 and the second flow channel 140 can be directly processed on the substrate 100.
[0044] In some embodiments of this application, the number of reaction chambers 110 is at least one, and each reaction chamber 110 has at least one cavity 120 disposed on its outer periphery. The different reaction chambers 110 can be arranged in series or in parallel. When multiple reaction chambers 110 are arranged in parallel, simultaneous amplification reactions of multiple samples can be achieved, improving detection efficiency.
[0045] In some embodiments of this application, the reaction chamber 110 is symmetrically arranged with respect to the connection line between the inlet and outlet ends. This allows for more uniform liquid flow within the reaction chamber 110, ensuring the consistency of the amplification reaction.
[0046] In some embodiments of this application, the reaction chamber 110 has a hexagonal outline. The hexagonal reaction chamber 110 can make fuller use of the space of the substrate 100, improving the utilization rate of the substrate 100. Simultaneously, the hexagonal reaction chamber 110 can also make the flow of liquid within the reaction chamber 110 more uniform, further improving the consistency of the amplification reaction.
[0047] An embodiment of the second aspect of this application provides a microfluidic chip, including the reaction chamber structure as described above. Therefore, the microfluidic chip provided by this application possesses all the beneficial effects of the aforementioned reaction chamber structure, which will not be repeated here.
[0048] It is understood that the reaction chamber structure and microfluidic chip provided in this application, by setting the cavity 120, can block the heat conduction from the reaction chamber 110 to the outside, thereby improving the efficiency of the amplification reaction within the reaction chamber 110. Simultaneously, the flat design of the reaction chamber 110 and the arc-shaped design of the cavity 120 can further ensure the reaction rate of the amplification reaction. Furthermore, the design of the number and contour of the reaction chambers 110 can also meet different detection requirements and improve detection efficiency.
[0049] It is worth understanding that the microfluidic chip of this application embodiment can be applied to fields including but not limited to the fields of microfluidic-based colloidal gold detection technology, immunofluorescence detection technology, chemiluminescence technology, and microfluidic-based nucleic acid detection technology.
[0050] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0051] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.
[0052] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0053] In the description of this specification, specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples.
[0054] It should be understood that although the steps in the flowcharts of the accompanying figures are shown sequentially as indicated by the arrows, these steps are not necessarily executed in the order indicated by the arrows. Unless explicitly stated herein, there is no strict order restriction on the execution of these steps, and they can be executed in other orders. Moreover, at least some steps in the flowcharts of the accompanying figures may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily completed at the same time, but can be executed at different times, and their execution order is not necessarily sequential, but can be performed alternately or in turn with other steps or at least some of the sub-steps or stages of other steps.
Claims
1. A reaction chamber structure, characterized in that, include: A substrate is provided with a reaction chamber and a cavity. The substrate has a first end face and a second end face disposed opposite to each other. The reaction chamber is disposed through the first end face and the second end face. At least one cavity is disposed on the outer periphery of the reaction chamber. A first sealing layer and a second sealing layer, wherein the first sealing layer covers the first end face and the second sealing layer covers the second end face.
2. The reaction chamber structure according to claim 1, characterized in that, The reaction chamber is flat in shape.
3. The reaction chamber structure according to claim 1, characterized in that, The cavity is arc-shaped.
4. The reaction chamber structure according to claim 3, characterized in that, The cavity is provided through the first end face and the second end face.
5. The reaction chamber structure according to any one of claims 1 to 4, characterized in that, The outer periphery of the reaction chamber is provided with four cavities spaced apart.
6. The reaction chamber structure according to claim 1, characterized in that, The reaction chamber has a liquid inlet and a liquid outlet. The substrate is provided with a first flow channel and a second flow channel. One end of the first flow channel is in fluid communication with the liquid inlet, and one end of the second flow channel is in communication with the liquid outlet. The first flow channel is located between one set of adjacent cavities, and the second flow channel is located between the other two cavities.
7. The reaction chamber structure according to claim 6, characterized in that, The reaction chamber is symmetrically arranged about the line connecting the inlet and outlet ends.
8. The reaction chamber structure according to claim 1, characterized in that, The number of reaction chambers is at least one, and each reaction chamber has at least one cavity on its outer periphery.
9. The reaction chamber structure according to claim 1, characterized in that, The reaction chamber has a hexagonal outline.
10. A microfluidic chip, characterized in that, Includes the reaction chamber structure as described in any one of claims 1 to 9.