Modularized stem cell preparation and extraction device

Abstract

The utility model provides a modularized stem cell preparation and extraction device which comprises a collecting tube, the open end of the collecting tube is detachably connected with a sealing cover, a first-stage centrifugal tube is arranged at the end, away from the opening, of the collecting tube, a plurality of first-stage centrifugal holes are formed in the outer wall of the first-stage centrifugal tube, and a first-stage ultrafiltration membrane is arranged in the first-stage centrifugal tube; the collecting tube is detachably connected with the collecting tube, a secondary centrifugal tube is arranged at one end, far away from an opening of the collecting tube, of the collecting tube, a plurality of secondary centrifugal holes are formed in the outer wall of the secondary centrifugal tube, and a secondary ultrafiltration membrane is arranged on the inner wall of the secondary centrifugal tube; the waste liquid tube is detachably connected with the taking centrifugal tube, and the opening end of the waste liquid tube is detachably connected with a waste liquid cover; the extraction tube is detachably connected with the taking centrifugal tube, and the opening end of the extraction tube is detachably connected with an extraction cover. According to the utility model, stem cells can be effectively extracted by adopting an ultrafiltration method, the filter membrane can be prevented from being blocked, the separation efficiency is improved, and the stem cells are easier to recover.

Description

Technical Field

[0001] This invention belongs to the field of cell separation and extraction technology, and relates to a modular stem cell preparation and extraction device. Background Technology

[0002] In the biomedical field, stem cells have attracted much attention due to their self-renewal capacity and ability to differentiate into various cell types. These unique properties make stem cells demonstrate enormous application potential in regenerative medicine, disease model establishment, and drug screening. To fully realize the application value of stem cells, the problem of their effective extraction and separation must first be solved. Stem cells can be derived from various tissues, including bone marrow, adipose tissue, and umbilical cord blood; however, how to efficiently extract a sufficient number of viable stem cells from these complex biological samples has become a key technical challenge.

[0003] Traditional stem cell extraction methods mainly include density gradient centrifugation and immunomagnetic bead sorting. While these methods can achieve stem cell separation and purification to a certain extent, they generally suffer from problems such as complex operation, high cost, and low recovery rate. With the development of materials science and engineering technology, ultrafiltration membrane technology has gradually been introduced into the stem cell extraction process. Ultrafiltration membrane technology is based on the principle of molecular sieves, achieving separation by selectively allowing molecules of different sizes to pass through. This method not only effectively removes impurities but also shows certain advantages in maintaining stem cell viability, thus it is considered an effective stem cell extraction method.

[0004] Despite significant advancements in ultrafiltration membrane technology for stem cell extraction, impurities such as proteins and cell debris in biological samples can easily clog the membrane pores during ultrafiltration. This not only affects separation efficiency but can also lead to a decrease in filtration speed. Furthermore, stem cells may adhere to the membrane surface or within the pores, making complete recovery difficult and further reducing stem cell extraction efficiency. Utility Model Content

[0005] The purpose of this invention is to provide a modular stem cell preparation and extraction device that uses ultrafiltration to effectively extract stem cells, prevent filter membrane clogging, improve separation efficiency, and make stem cells easier to recover.

[0006] To solve the above-mentioned technical problems, this utility model provides a modular stem cell preparation and extraction device, including a collection tube, the open end of which is detachably connected to a sealing cap, a primary centrifuge tube is provided at the end of the collection tube away from its opening, the outer diameter of the primary centrifuge tube is smaller than the outer diameter of the collection tube, a plurality of primary centrifuge holes are opened on the outer wall of the primary centrifuge tube, and a primary ultrafiltration membrane is provided inside the primary centrifuge tube.

[0007] It also includes a collection centrifuge tube detachably connected to the collection tube. A secondary centrifuge tube is provided at the end of the collection centrifuge tube away from its opening. The outer diameter of the secondary centrifuge tube is smaller than that of the collection centrifuge tube. The primary centrifuge tube can extend into the secondary centrifuge tube. The outer wall of the secondary centrifuge tube has several secondary centrifuge holes. The inner wall of the secondary centrifuge tube is provided with multiple spiral guide strips distributed in a circle. A spiral secondary ultrafiltration membrane is provided between every two adjacent spiral guide strips on the inner wall of the secondary centrifuge tube. An extraction hole is provided at the free end of the secondary centrifuge tube. The extraction hole is detachably connected to a sealing plug.

[0008] It also includes a waste liquid tube detachably connected to the collection centrifuge tube, the secondary centrifuge tube being able to extend into the waste liquid tube, and the open end of the waste liquid tube being detachably connected to a waste liquid cap;

[0009] It also includes an extraction tube detachably connected to the collection centrifuge tube, wherein the secondary centrifuge tube can extend into the extraction tube, and the opening end of the extraction tube is detachably connected to an extraction cap.

[0010] By employing the above technical solution, tissue samples (such as fat, umbilical cord, and placenta) are dispersed using enzymatic digestion (such as collagenase) and added to a collection tube. A collection centrifuge tube is connected to the collection tube, and a waste liquid tube is connected to the collection centrifuge tube. The samples are then placed in a centrifuge. During centrifugation, because stem cells are 10-30 μm in size, stem cells and smaller cells pass through the primary ultrafiltration membrane in the primary centrifuge tube into the secondary centrifuge tube. Cells larger than stem cells or tissue fragments remain in the primary centrifuge tube. Under continued centrifugation, smaller cells pass through the secondary ultrafiltration membrane in the secondary centrifuge tube. The first-stage ultrafiltration membrane enters the waste liquid tube, while the stem cells are collected in the second-stage centrifuge tube. Then, the device is removed, the waste liquid tube is taken off, the sealing plug is removed, the extraction tube is connected to the collection centrifuge tube, and the device is placed in a centrifuge for centrifugation. Under centrifugation, the stem cells gradually approach the spiral guide strip and enter the extraction tube through the extraction hole along the spiral guide strip. During the process, the stem cells attached to the second-stage ultrafiltration membrane can also gradually detach under centrifugation and enter the extraction tube. Finally, the device is removed from the centrifuge, the extraction tube is removed, the extraction tube is capped, and the extraction tube is left to stand to allow the stem cells to settle to the bottom, thus completing the preparation and extraction.

[0011] The present invention is further configured such that the outer diameter of the primary centrifuge tube gradually decreases in the direction away from the collection tube, and the outer diameter of the secondary centrifuge tube gradually decreases in the direction away from the retention centrifuge tube.

[0012] The present invention is further configured such that the outer wall of the collection tube is provided with a primary threaded connection section with external threads, and the inner wall of the retention centrifuge tube is provided with an internal thread that is threadedly connected to the primary threaded connection section.

[0013] The present invention is further configured such that the outer wall of the centrifuge tube is provided with a secondary threaded connection section with external threads, the inner walls of the waste liquid tube and the extraction tube are provided with internal threads that are threadedly connected to the secondary threaded connection section, the waste liquid cap is threadedly connected to the waste liquid tube, and the extraction cap is threadedly connected to the extraction tube.

[0014] The present invention is further configured such that the sealing cap is threadedly connected to the collection tube.

[0015] The present invention is further configured such that the spiral guide bar has 0.2-0.4 spiral turns.

[0016] The present invention is further provided that the outer end of the sealing plug is provided with a protrusion.

[0017] The present invention is further configured such that the pore size of the primary ultrafiltration membrane is 30-50 μm, and the pore size of the secondary ultrafiltration membrane is 8-10 μm.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] Firstly, this utility model uses a modular collection tube, a collection centrifuge tube, a waste liquid tube, and an extraction tube to separate and extract stem cells through a step-by-step centrifugation and ultrafiltration process. Larger cells are filtered into the collection tube, stem cells are collected into the collection centrifuge tube, and smaller cells are filtered into the waste liquid tube, so as to achieve rapid filtration of stem cells through ultrafiltration.

[0020] Secondly, this invention features a spiral guide strip inside the secondary centrifuge tube. Under centrifugal force, the stem cells inside gradually come into contact with the spiral guide strip and move downwards with it, entering the extraction tube through the extraction hole. During this process, the stem cells attached to the secondary ultrafiltration membrane can also gradually detach under centrifugation, effectively recovering the stem cells attached to the surface of the filter membrane and further improving the extraction efficiency of stem cells. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 It is a partial cross-sectional view used to show the internal structure of the collection tube and the first-stage centrifuge tube;

[0023] Figure 3 This is a partial sectional view used to show the internal structure of the collection centrifuge tube and the secondary centrifuge tube;

[0024] Figure 4 Used to demonstrate the connection between the waste liquid pipe and the waste liquid cap;

[0025] Figure 5Used to demonstrate the connection between the extraction tube and the extraction cap.

[0026] The components are as follows: 1. Collection tube; 2. Sealing cap; 3. Primary threaded connection section; 4. Primary centrifuge tube; 5. Primary centrifuge hole; 6. Primary ultrafiltration membrane; 7. Collection centrifuge tube; 8. Secondary threaded connection section; 9. Secondary centrifuge tube; 10. Secondary centrifuge hole; 11. Spiral guide strip; 12. Secondary ultrafiltration membrane; 13. Extraction hole; 14. Sealing plug; 15. Raised strip; 16. Waste liquid tube; 17. Waste liquid cap; 18. Extraction tube; 19. Extraction cap. Detailed Implementation

[0027] The modular stem cell preparation and extraction device proposed in this utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments. The advantages and features of this utility model will become clearer from the following description. It should be noted that the drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of this utility model. The same or similar reference numerals in the drawings represent the same or similar parts.

[0028] Example, refer to Figure 1-5 A modular stem cell preparation and extraction device includes a collection tube 1, with a sealing cap 2 threadedly connected to the open end of the collection tube 1. The outer wall of the collection tube 1 is provided with a primary threaded connection section 3 with external threads. A primary centrifuge tube 4 is provided at the end of the collection tube 1 away from its opening. The outer diameter of the primary centrifuge tube 4 gradually decreases in the direction away from the collection tube 1, and the outer diameter of the primary centrifuge tube 4 is smaller than that of the collection tube 1. Several primary centrifuge holes 5 are opened on the outer wall of the primary centrifuge tube 4. A primary ultrafiltration membrane 6 is provided inside the primary centrifuge tube 4, and the pore size of the primary ultrafiltration membrane 6 is 30-50 μm.

[0029] It also includes a collection centrifuge tube 7 detachably connected to the collection tube 1. The inner wall of the collection centrifuge tube 7 is provided with an internal thread that connects to the primary threaded connection section 3. The outer wall of the collection centrifuge tube 7 is provided with a secondary threaded connection section 8 with external threads. A secondary centrifuge tube 9 is provided at the end of the collection centrifuge tube 7 away from its opening. The outer diameter of the secondary centrifuge tube 9 gradually decreases in the direction away from the collection centrifuge tube 7. The outer diameter of the secondary centrifuge tube 9 is smaller than the outer diameter of the collection centrifuge tube 7. The primary centrifuge tube 4 can extend into the secondary centrifuge tube 9. The outer wall of the secondary centrifuge tube 9 is open. The centrifuge tube 9 is provided with several secondary centrifuge holes 10. The inner wall of the secondary centrifuge tube 9 is provided with four spiral guide strips 11 arranged in a circular pattern. The spiral guide strips 11 have 0.2-0.4 turns. Between each two adjacent spiral guide strips 11, a spiral secondary ultrafiltration membrane 12 is provided on the inner wall of the secondary centrifuge tube 9. The pore size of the secondary ultrafiltration membrane 12 is 8-10μm. An extraction hole 13 is opened at the free end of the secondary centrifuge tube 9. A sealing plug 14 is detachably connected to the extraction hole 13. A protrusion 15 is provided at the outer end of the sealing plug 14.

[0030] It also includes a waste liquid pipe 16 that is detachably connected to the centrifuge tube 7. The inner wall of the waste liquid pipe 16 is provided with internal threads that are threaded to the secondary threaded connection section 8. The secondary centrifuge tube 9 can extend into the waste liquid pipe 16. The open end of the waste liquid pipe 16 is threadedly connected to a waste liquid cap 17.

[0031] It also includes an extraction tube 18 that is detachably connected to the centrifuge tube 7. The inner wall of the extraction tube 18 is provided with internal threads that are threaded to the secondary threaded connection section 8. The secondary centrifuge tube 9 can extend into the extraction tube 18. An extraction cap 19 is threadedly connected to the open end of the extraction tube 18.

[0032] Working principle: Tissue samples (such as fat, umbilical cord, placenta) are dispersed using enzymatic digestion (such as collagenase) and added to collection tube 1. Centrifuge tube 7 is connected to collection tube 1, and waste liquid tube 16 is connected to centrifuge tube 7. The samples are then centrifuged. During centrifugation, stem cells (10-30 μm in size) and smaller cells pass through the primary ultrafiltration membrane 6 in the primary centrifuge tube 4 into the secondary centrifuge tube 9. Cells larger than stem cells or tissue fragments remain in the primary centrifuge tube 4. With continued centrifugation, smaller cells pass through the secondary ultrafiltration membrane 12 in the secondary centrifuge tube 9 into the waste liquid tube. The stem cells are collected in the secondary centrifuge tube 9, and then the device is removed. The waste liquid tube 16 is removed, the sealing plug 14 is removed, and the extraction tube 18 is connected to the collection centrifuge tube 7. The device is then placed in a centrifuge for centrifugation. Under centrifugation, the stem cells gradually approach the spiral guide strip 11 and enter the extraction tube 18 through the extraction hole 13 along the spiral guide strip 11. During the process, the stem cells attached to the secondary ultrafiltration membrane 12 can also gradually detach and enter the extraction tube 18 under centrifugation. Finally, the device is removed from the centrifuge, the extraction tube 18 is removed, the extraction tube 18 is covered, and the extraction tube 18 is left to stand to allow the stem cells to settle to the bottom and complete the preparation and extraction.

[0033] It should also be noted that all terms such as "set up" and similar descriptive words in this application (especially the specification) indicate that two structures have or exist a connection relationship. However, the specific means by which the two are connected are not limited in detail, and are usually conventional connection methods. That is, the means should be understood as prior art and do not need to be elaborated. For example, "m is set up with n" only indicates that structure m has structure n, and whether the two are connected by welding, riveting, adhesive, or integral molding is within the scope of protection of this application. Similarly, "x is rotatably set up with y" only indicates that y and x can rotate relative to each other, and whether the two are connected by a bearing, or whether y directly passes through x and is rotatably connected to x, or other feasible methods, are all within the scope of protection of this application.

[0034] The above description is only a description of the preferred embodiment of the present utility model and is not intended to limit the scope of the present utility model in any way. Any changes or modifications made by those skilled in the art based on the above disclosure shall fall within the protection scope of the claims.

Claims

1. A modular stem cell preparation and extraction device, comprising a collection tube (1), wherein the open end of the collection tube (1) is detachably connected to a sealing cap (2), characterized in that, The collection tube (1) is provided with a first-stage centrifuge tube (4) at one end away from its opening. The outer diameter of the first-stage centrifuge tube (4) is smaller than the outer diameter of the collection tube (1). The outer wall of the first-stage centrifuge tube (4) is provided with several first-stage centrifuge holes (5). The first-stage centrifuge tube (4) is provided with a first-stage ultrafiltration membrane (6). It also includes a collection centrifuge tube (7) detachably connected to the collection tube (1). A secondary centrifuge tube (9) is provided at the end of the collection centrifuge tube (7) away from its opening. The outer diameter of the secondary centrifuge tube (9) is smaller than the outer diameter of the collection centrifuge tube (7). The primary centrifuge tube (4) can extend into the secondary centrifuge tube (9). The outer wall of the secondary centrifuge tube (9) is provided with several secondary centrifuge holes (10). The inner wall of the secondary centrifuge tube (9) is provided with several spiral guide strips (11) arranged in a circular pattern. A spiral secondary ultrafiltration membrane (12) is provided between every two adjacent spiral guide strips (11) on the inner wall of the secondary centrifuge tube (9). An extraction hole (13) is provided at the free end of the secondary centrifuge tube (9). The extraction hole (13) is detachably connected with a sealing plug (14). It also includes a waste liquid tube (16) detachably connected to the collection centrifuge tube (7), the secondary centrifuge tube (9) being able to extend into the waste liquid tube (16), and the open end of the waste liquid tube (16) being detachably connected to a waste liquid cap (17); It also includes an extraction tube (18) detachably connected to the collection centrifuge tube (7), wherein the secondary centrifuge tube (9) can extend into the extraction tube (18), and the opening end of the extraction tube (18) is detachably connected to an extraction cap (19).

2. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The outer diameter of the primary centrifuge tube (4) gradually decreases in the direction away from the collection tube (1), and the outer diameter of the secondary centrifuge tube (9) gradually decreases in the direction away from the retention centrifuge tube (7).

3. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The outer wall of the collection tube (1) is provided with a first-stage threaded connection section (3) with external threads, and the inner wall of the centrifuge tube (7) is provided with an internal thread that is threadedly connected to the first-stage threaded connection section (3).

4. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The outer wall of the centrifuge tube (7) is provided with a secondary threaded connection section (8) with external threads. The inner walls of the waste liquid tube (16) and the extraction tube (18) are provided with internal threads that are threaded to the secondary threaded connection section (8). The waste liquid cap (17) is threaded to the waste liquid tube (16), and the extraction cap (19) is threaded to the extraction tube (18).

5. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The sealing cap (2) is threadedly connected to the collection tube (1).

6. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The spiral guide bar (11) has 0.2-0.4 spiral turns.

7. The modular stem cell preparation and extraction device according to claim 1, characterized in that, The outer end of the sealing plug (14) is provided with a protrusion (15).

8. A modular stem cell preparation and extraction device according to any one of claims 1-7, characterized in that, The pore size of the primary ultrafiltration membrane (6) is 30-50 μm, and the pore size of the secondary ultrafiltration membrane (12) is 8-10 μm.

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