Cell transfer device

Abstract

The utility model discloses a cell transfer device which comprises a needle holding handle and a handle clamping universal seat, a transfer needle is mounted at one end of the needle holding handle, the other end of the needle holding handle is connected with a microinjector through a pipeline, and a needle cavity of the transfer needle is communicated with a pipe cavity of the needle holding handle; the handle clamping universal seat comprises a strip-shaped seat, a handle support, a balance weight base, a ball head bolt, a spherical hinge and a resistance adjusting assembly. According to the utility model, cells containing liquid can be conveniently sucked and transferred.

Description

Technical Field

[0001] This utility model relates to the field of assisted reproductive technology, specifically a cell transfer device. Background Technology

[0002] Assisted reproductive technology (in vitro fertilization and embryo transfer) has become an important means of solving infertility.

[0003] In vitro fertilization and embryo transfer (IVF) is a technique used by infertile couples who have been medically evaluated and deemed suitable for the procedure. The woman undergoes ovulation induction treatment to retrieve mature eggs, while the man retrieves sperm to fertilize the eggs. Once an embryo is formed, it is transferred into the uterus, or any excess embryos are cryopreserved, thereby achieving pregnancy.

[0004] With the rapid development of genetics and gene sequencing technologies, preimplantation genetic testing (PGT) has become widely used. PGT, also known as third-generation in vitro fertilization (IVF), refers to the use of genetic techniques to test the polar bodies of biopsied oocytes or embryonic cells before embryo transfer into the uterine cavity. The genetic information of the test data is analyzed to determine the chromosomal or gene status of the oocytes or embryos. Embryos that do not show specific variations or whose genetic traits caused by specific variations are within acceptable limits are selected for transfer into the uterine cavity, ultimately leading to the birth of healthy offspring.

[0005] The consensus among Chinese experts on assisted reproductive technology indicates that indications for PGT technology include: single-gene disorders, mitochondrial diseases, HLA typing, severe genetic susceptibility diseases with high penetrance, chromosomal structural abnormalities, and special circumstances such as advanced age, recurrent implantation failure, and recurrent miscarriage. Therefore, the application of PGT technology in assisted reproductive technology is becoming increasingly widespread.

[0006] One of the key steps in PGT technology is embryo biopsy. When an embryo develops to the blastocyst stage, it is transferred to an embryo biopsy dish. Under an inverted microscope, the blastocyst is fixed with a micromanipulation device and a hole is made in the zona pellucida of the blastocyst using a laser. Then, 5-8 trophoblast cells are removed from the trophoblast layer using the embryo biopsy needle of the micromanipulation device. The embryo biopsy dish is then transferred to a stereomicroscope, and the liquid containing 5-8 trophoblast cells is transferred to a PCR tube under a thin Pasteur dropper.

[0007] A cluster of 5-8 trophoblast cells is approximately 50 μm in diameter. Under a microscope, transferring these cells to a PCR tube requires precise manipulation. First, the glass tip of the Pasteurizer needs to be aligned with the cells to be transferred. Second, the fingers must meticulously control the latex tip of the Pasteurizer to aspirate the liquid containing the trophoblast cells. Only a very small amount of liquid can be aspirated each time, and this delicate operation often results in involuntary hand tremors. Especially when multiple embryos require biopsy and cell transfer, the increased workload and hand fatigue make precise control even more difficult, increasing the likelihood of tremors. Once tremors occur, the procedure is prone to failure, resulting in the inability to aspirate cells, the needle tip touching or squeezing cells, or cell loss during transfer. This leads to the failure of the entire PGT technique, causing significant economic and psychological burdens for the patient. Utility Model Content

[0008] The purpose of this invention is to provide a cell transfer device to solve the above-mentioned technical problems.

[0009] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0010] A cell transfer device, comprising:

[0011] The needle holder has a transfer needle installed at one end and a microinjector connected to the other end via a tube. The cavity of the transfer needle is connected to the cavity of the needle holder.

[0012] The handle clamping universal joint includes a strip base, a handle bracket, a counterweight base, a ball head bolt, a ball joint, and a resistance adjustment assembly;

[0013] The strip seat body is provided with a sliding groove;

[0014] The handle bracket is used to hold the needle holder handle, and the bottom of the handle bracket is provided with a slide rail that slides in conjunction with the slide groove;

[0015] The counterweight base is located below the strip base, and the ball head bolt is provided on the counterweight base. The ball head bolt is engaged with the ball joint provided on the strip base.

[0016] The resistance adjustment component is located at the bottom of the slide groove and is used to increase or decrease the moving resistance of the slide rail when it slides in the slide groove.

[0017] Furthermore, the resistance adjustment component includes multiple sets of ball screws distributed in a straight line along the guide rail at the bottom of the slide groove. The ball screws are threaded into the threaded holes provided on the strip seat body, and the ball surfaces of the ball screws roll against the bottom surface of the slide rail.

[0018] Furthermore, the handle bracket is strip-shaped, with a groove on its body to accommodate the needle handle tube, and a locking bolt to lock the position of the needle handle tube located in the groove.

[0019] Furthermore, the handle bracket body is also provided with a slot to break the groove.

[0020] Furthermore, the slide groove and slide rail are dovetail shaped.

[0021] Furthermore, a limiting block is provided at one end of the strip seat.

[0022] Furthermore, the bottom surface of the counterweight base is provided with anti-slip pads.

[0023] The beneficial effects of this utility model are:

[0024] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0025] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0026] Figure 1 This is a perspective view of the present invention.

[0027] Figure 2 This is a perspective view of the universal joint for holding the handle of this utility model.

[0028] Figure 3 This is an exploded view of the universal joint holding handle of this utility model.

[0029] Figure 4 This is a three-dimensional view showing the internal structural lines of the universal joint holding handle of this utility model.

[0030] In the diagram: 1. Needle holder handle, 2. Transfer needle, 300. Strip seat, 301. Handle bracket, 302. Counterweight base, 303. Ball head bolt, 304. Ball joint, 305. Slide groove, 306. Slide rail, 307. Bead screw, 308. Groove, 309. Locking bolt, 310. Slot, 311. Limiting block, 312. Anti-slip pad. Detailed Implementation

[0031] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0032] Reference Figures 1-4 As shown in the embodiment provided by this utility model, a cell transfer device includes a needle handle 1, a transfer needle 2, and a handle clamping universal seat.

[0033] One end of the needle handle 1 is threadedly connected to a transfer needle 2 and sealed with a sealing ring. The other end is connected to a microinjector via a tube, and the cavity of the transfer needle 2 communicates with the cavity of the needle handle 1. Using the microinjector, the transfer needle 2 can aspirate liquid containing trophoblast cells and store it at the tip of the transfer needle tube. The amount aspirated in a single operation can be observed under a microscope and precisely controlled by the microinjector. During liquid transfer, there is no leakage or cell loss, avoiding the risk of unintentional force applied by the fingers to the latex tip of the Pasteur dropper, which could cause liquid leakage and potentially cell loss, as is common when using a Pasteur dropper.

[0034] The handle clamping universal joint includes a strip base 300, a handle bracket 301, a counterweight base 302, a ball head bolt 303, a ball joint 304, and a resistance adjustment assembly;

[0035] The strip seat 300 has a sliding groove 305 on its main body;

[0036] The handle bracket 301 is used to hold the needle holder handle 2. The bottom of the handle bracket 301 is provided with a slide rail 306 that slides with the slide groove 305. Both the slide groove 305 and the slide rail 306 are dovetail-shaped. A limiting block 311 is provided at one end of the strip seat 300. The limiting block 311 is used to limit the slide rail 306 from continuing to move forward after it moves to the end of the slide groove 305.

[0037] The counterweight base 302 is located below the strip base 300. The counterweight base 302 is provided with a ball head bolt 303, which cooperates with the ball joint 304 provided on the strip base 300. The bottom surface of the counterweight base 302 is provided with an anti-slip pad 312.

[0038] With the cooperation of ball head bolt 303 and ball joint 304, bar seat 300 forms universal seat. Therefore, when the operator needs to adjust the angle during the experiment, he only needs to swing bar seat 300 or handle bracket 301 connected to bar seat 300. The needle holder 1 held on handle bracket 301 will also swing and be adjusted to the angle required for working. Furthermore, during the experiment, the universal support formed by the strip holder 300 avoids the influence of hand tremors, allowing the transfer needle 2 at the end of the needle handle 1 to be smoothly aligned with the vicinity of the cells to be transferred in the biopsy dish. Then, the microinjector is manipulated to draw liquid containing trophoblast cells into the lumen of the transfer needle. The manipulation of the microinjector is stopped, and the cells and liquid remain still in the transfer needle. Then, the strip holder 300 is swung to lift one end of the transfer needle, and the biopsy dish is removed from the microscope. The PCR tube is then placed in an appropriate position under the microscope objective. The strip holder 300 and the handle support 301 are swung to insert the transfer needle 2 into the bottom of the PCR tube. Only then can the microinjector be manipulated to blow the cells and a small amount of liquid out of the transfer needle and into the PCR tube. Using this device, the cell transfer process can be clearly observed under a microscope, improving cell safety and the success rate of transfer.

[0039] The resistance adjustment component is located at the bottom of the slide groove 305, and it is used to increase or decrease the moving resistance of the slide rail 306 when it slides in the slide groove 305.

[0040] Specifically, the resistance adjustment component includes multiple sets of ball screws 307 arranged linearly along the guide rail 305 at the bottom of the groove 305. The ball screws 307 are threaded into threaded holes on the main body of the strip seat 300, and the spherical balls of the ball screws 307 roll against the bottom surface of the slide rail 306. By rotating the ball screws 307 in the threaded holes on the main body of the strip seat 300, the contact force between the spherical balls of the ball screws 307 and the bottom surface of the slide rail 306 can be adjusted, thereby adjusting the movement resistance of the slide rail 306 when sliding within the groove 305. The compressive force between the spherical balls of the ball screws 307 and the bottom surface of the slide rail 306 restricts the slide rail 306 from moving freely within the groove 305, preventing displacement during angle adjustment of the handle bracket 301 and thus avoiding unnecessary shaking.

[0041] Specifically, the handle bracket 301 is strip-shaped, with a groove 308 on its body to accommodate the tube of the needle handle 2, and a locking bolt 309 to lock the position of the tube of the needle handle 2 located in the groove. The handle bracket 301 also has a slot 310 that breaks the groove 308. At the position of the slot 310, it is convenient for the operator to directly remove the needle handle 2 by hand.

[0042] The embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A cell transfer device, characterized in that, include: The needle handle (1) has a transfer needle (2) installed at one end and a microinjector connected to the other end through a tube. The cavity of the transfer needle (2) is connected to the cavity of the needle handle (1). The handle clamping universal joint includes a strip base (300), a handle bracket (301), a counterweight base (302), a ball head bolt (303), a ball joint (304), and a resistance adjustment assembly; The strip seat (300) body is provided with a sliding groove (305); The handle bracket (301) is used to hold the needle handle (1), and the bottom of the handle bracket (301) is provided with a slide rail (306) that slides in cooperation with the slide groove (305). The counterweight base (302) is located below the strip seat (300), and the counterweight base (302) is provided with the ball head bolt (303), which cooperates with the ball joint (304) provided on the strip seat (300); The resistance adjustment component is located at the bottom of the slide groove (305) and is used to increase or decrease the moving resistance of the slide rail (306) when it slides in the slide groove (305).

2. The cell transfer device according to claim 1, characterized in that... The resistance adjustment component includes multiple sets of ball screws (307) distributed along the guide line of the slide groove (305) at the bottom of the slide groove (305). The ball screws (307) are threaded into the threaded holes provided on the body of the strip seat (300), and the ball spherical surface of the ball screws (307) rolls against the bottom surface of the slide rail (306).

3. The cell transfer device according to claim 1, characterized in that... The handle bracket (301) is strip-shaped, and its body has a groove (308) for accommodating the tube of the needle handle (1), and a locking bolt (309) for locking the position of the tube of the needle handle (1) located in the groove.

4. The cell transfer device according to claim 3, characterized in that... The handle bracket (301) body is also provided with a slot (310) to break the groove (308).

5. The cell transfer device according to claim 1, characterized in that... The slide groove (305) and slide rail (306) are dovetail-shaped.

6. The cell transfer device according to claim 1, characterized in that... One end of the strip seat (300) is provided with a limiting block (311).

7. The cell transfer device according to claim 1, characterized in that... The counterweight base (302) is provided with anti-slip pads (312) on its bottom surface.

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