Stable high-throughput microsphere culture sheet
By designing C-shaped fixing rings and counterweights on the culture plates, the problem of culture plates floating in the culture medium was solved, thus achieving the stability of cell aggregates and the reliability of culture results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- QIJIA TECH (SUZHOU) CO LTD
- Filing Date
- 2025-04-16
- Publication Date
- 2026-05-01
AI Technical Summary
Cell culture slides tend to float in the culture medium, affecting cell aggregation and leading to unstable culture results.
A stable high-throughput microsphere culture plate was designed, employing a C-shaped fixing ring and a counterweight. The fixing ring consists of an arc segment and a wavy segment. The wavy segment is elastic and can hold the culture plate in place within the well plate. The counterweight adds weight to prevent floating.
Effective fixation of culture plates prevents them from floating in the culture medium, ensuring the stability of cell aggregates and improving the reliability of culture results.
Smart Images
Figure CN224186181U_ABST
Abstract
Description
A stable high-throughput microsphere culture sheet Technical Field
[0001] This utility model relates to the field of biological experimental equipment, and in particular to a stable high-throughput microsphere culture plate. Background Technology
[0002] Cell culture slides are frequently used in research and development, particularly in the life sciences and pharmaceutical industries. They have specific applications in high-yield in vitro cell culture, cell assays, and cell analysis, such as culturing 3D cell microspheres. When using cell culture slides, they are first placed in a 24-well plate, and then the culture medium is added. Because the size of the slide is smaller than the well size of the 24-well plate, it will float in the culture medium after addition. This may cause the slide to shake during culture, which is detrimental to cell aggregation and thus affects the acquisition of cell culture results. Summary of the Invention
[0003] In view of this, the present invention aims to provide a stable high-throughput microsphere culture plate to solve the above problems.
[0004] To achieve the above objectives, the technical solution of this utility model is implemented as follows:
[0005] A stable high-throughput microsphere culture plate includes a culture plate body, a lifting column, and a fixing ring. The lifting column is fixed in the center of the culture plate body. The upper surface of the culture plate body has several grooves, and the bottom of the grooves is V-shaped. A C-shaped fixing ring is fitted around the outer periphery of the culture plate body. A limiting ring is provided on the outer periphery of the culture plate body. The inner sidewall of the fixing ring has a groove that cooperates with the limiting ring. The fixing ring includes an integrally connected arc segment and a wavy segment. The distance from the center of the culture plate body to the crest of the wavy segment is greater than or equal to the radius of the hole in the well plate where the culture plate is located.
[0006] Furthermore, the wave segment is divided into two parts, which are alternated with the arc segment, and the two wave segments are symmetrically arranged.
[0007] Furthermore, the side wall of the lifting column is provided with annular recesses.
[0008] Furthermore, the included angle of the V-shape at the bottom of the groove is 45-145°.
[0009] Furthermore, the diameter of the groove is 0.5-5mm and the depth is 0.3-2mm.
[0010] Furthermore, a counterweight is also provided at the bottom of the culture plate.
[0011] Furthermore, the counterweight includes a counterweight plate and an insert connected to the counterweight plate, and the bottom of the culture plate body is provided with a fixing groove for inserting the insert.
[0012] Furthermore, a limiting block protrudes from the side wall of the insertion post, and a limiting groove is provided on the side wall of the fixing groove to facilitate the insertion of the limiting block. An arc-shaped sliding groove communicating with the limiting groove is also provided on the side wall of the fixing groove, and the limiting block can slide in the sliding groove.
[0013] Furthermore, the counterweight plate contains a counterweight.
[0014] Furthermore, there are four counterweights, which are evenly distributed at the bottom of the culture plate body.
[0015] Compared with existing technologies, the stable high-throughput microsphere culture plate of this invention has the following advantages:
[0016] The stable high-throughput microsphere culture described in this invention features a C-shaped retaining ring fitted around the periphery of the culture plate. This retaining ring comprises an integrally connected arc segment and a wavy segment. The wavy segment of the retaining ring has a certain elasticity. When the culture plate is placed in the corresponding well plate, the crest of the wavy segment contacts and is compressed against the well wall, causing elastic deformation. The C-shaped retaining ring, due to its notch, facilitates deformation. Therefore, the wavy segment of the retaining ring can hold the culture plate in place within the well, preventing floating due to added culture medium and thus avoiding interference with microsphere aggregation. Furthermore, to ensure the culture plate does not float, a counterweight can be added to the bottom as needed to increase the weight of the culture plate and reduce floating. Attached Figure Description
[0017] The accompanying drawings, which form part of this utility model, are used to provide a further understanding of the utility model. The illustrative embodiments of the utility model and their descriptions are used to explain the utility model and do not constitute an undue limitation of the utility model. In the drawings:
[0018] Figure 1 is a schematic diagram of the structure of the stable high-throughput microsphere culture sheet according to an embodiment of the present invention;
[0019] Figure 2 is a cross-sectional view of the stable high-throughput microsphere culture plate described in the embodiment of this utility model;
[0020] Figure 3 is a structural schematic diagram of the counterweight according to an embodiment of the present utility model;
[0021] Figure 4 is a bottom structure diagram of the stable high-throughput microsphere culture plate described in the embodiment of this utility model;
[0022] Figure 5 is a schematic diagram of the structure of the fixing ring according to an embodiment of the present invention.
[0023] Explanation of reference numerals in the attached figures:
[0024] 1. Culture plate body; 11. Edge; 12. Groove; 13. Limiting ring; 14. Fixing groove; 15. Limiting groove; 16. Sliding groove; 2. Lifting column; 21. Recess; 3. Fixing ring; 31. Arc segment; 32. Wavy segment; 33. Slot; 4. Counterweight; 41. Counterweight plate; 42. Insert column; 421. Limiting block; 43. Counterweight. Detailed Implementation
[0025] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.
[0026] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.
[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0028] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.
[0029] As shown in Figures 1-5, a stable high-throughput microsphere culture plate includes a culture plate body 1, a lifting column 2, and a fixing ring 3. The lifting column 2 is fixed in the center of the culture plate body 1 for easy access to the culture plate. The upper surface of the culture plate body 1 is provided with several grooves 12, the bottom of which is V-shaped, which is more conducive to cell aggregation. A C-shaped fixing ring 3 is fitted around the outer periphery of the culture plate body 1. A limiting ring 13 is provided around the outer periphery of the culture plate body 1. The inner sidewall of the fixing ring 3 is provided with a slot 33 that cooperates with the limiting ring 13. The fixing ring 3 includes an integrally connected arc segment 31 and a wavy segment 32. The distance from the center of the culture plate body 1 to the crest of the wavy segment 32 is greater than or equal to the radius of the well plate in which the culture plate is located.
[0030] The wavy retaining ring has a certain elasticity. When the culture plate is placed in the well of a corresponding plate, such as a 24-well plate, the crest of the wavy section contacts and is compressed against the well wall, causing elastic deformation. The C-shaped retaining ring, due to its notch, facilitates deformation. Therefore, the wavy section of the retaining ring can hold the culture body in the well, preventing it from floating due to the addition of culture medium and affecting the aggregation of the pellets.
[0031] For example, the wave segment 32 has two segments, which are alternately arranged with the arc segment 31, and the two wave segments 32 are arranged symmetrically.
[0032] For example, the side wall of the lifting column 2 is provided with an annular recess 21. The recess facilitates the gripping of the culture slide with tweezers and serves as a limit to prevent it from slipping during the gripping process.
[0033] For example, the included angle of the V-shape at the bottom of the groove 12 is 45-145°. Specifically, it can be 100°.
[0034] For example, the diameter of the groove 12 is 0.5-5mm and the depth is 0.3-2mm.
[0035] The diameter can be 0.5mm, 1mm, 2mm, 3mm, 4mm or 5mm, or any other value between 0.5 and 5mm. No specific limit is set here, and it depends on the actual situation.
[0036] The depth can be 0.3mm, 1mm, 1.5mm, or 2mm, or any other value between 0.3mm and 2mm. No specific limit is set here; it depends on the actual situation.
[0037] For example, in order to ensure that the culture plate does not float, a counterweight can be set at the bottom as needed to increase the weight of the culture plate itself and reduce the floating phenomenon. The bottom of the culture plate body 1 is also provided with a counterweight 4.
[0038] Specifically, the counterweight 4 includes a counterweight plate 41 and an insertion post 42 connected to the counterweight plate 41, and the bottom of the culture plate body 1 is provided with a fixing groove 14 for inserting the insertion post 42.
[0039] More specifically, a limiting block 421 protrudes from the side wall of the insertion post 42, and a limiting groove 15 is provided on the side wall of the fixing groove 14 to facilitate the insertion of the limiting block 421. An arc-shaped sliding groove 16 communicating with the limiting groove 15 is also provided on the side wall of the fixing groove 14, and the limiting block 421 can slide in the sliding groove 16.
[0040] The counterweights are detachable and can be selected for use as needed.
[0041] When it is necessary to insert a counterweight, align the limiting block with the limiting groove, align the insert post with the fixing groove, insert the insert post, and then rotate the insert post to make the limiting block slide along the sliding groove, thereby causing the limiting block and the limiting groove to be misaligned and locking the counterweight.
[0042] Specifically, the counterweight plate 41 contains a counterweight 43.
[0043] For example, there are four counterweights 4, which are evenly distributed at the bottom of the culture plate body 1.
[0044] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A stable high-throughput microsphere culture sheet, characterized in that: The device includes a culture plate body, a lifting column, and a retaining ring. The lifting column is fixed in the center of the culture plate body. The upper surface of the culture plate body has several grooves, and the bottom of the grooves is V-shaped. A C-shaped retaining ring is fitted around the outer periphery of the culture plate body. A limiting ring is provided on the outer periphery of the culture plate body. The inner side wall of the retaining ring has a slot that cooperates with the limiting ring. The retaining ring includes an integrally connected arc segment and a wavy segment. The distance from the center of the culture plate body to the crest of the wavy segment is greater than or equal to the radius of the hole in the well plate where the culture plate is located.
2. The stable high-throughput microsphere culture sheet according to claim 1, characterized in that: The wave segment consists of two segments, which alternate with the arc segment, and the two wave segments are arranged symmetrically.
3. The stable high-throughput microsphere culture sheet according to claim 1, characterized in that: The side wall of the lifting column has an annular recess.
4. The stable high-throughput microsphere culture sheet according to claim 1, characterized in that: The included angle of the V-shape at the bottom of the groove is 45-145°.
5. The stable high-throughput microsphere culture sheet according to claim 1, characterized in that: The diameter of the groove is 0.5-5mm and the depth is 0.3-2mm.
6. The stable high-throughput microsphere culture sheet according to claim 1, characterized in that: The bottom of the culture plate is also equipped with a counterweight.
7. The stable high-throughput microsphere culture sheet according to claim 6, characterized in that: The counterweight includes a counterweight plate and an insert connected to the counterweight plate. The bottom of the culture plate body is provided with a fixing groove for inserting the insert.
8. The stable high-throughput microsphere culture sheet according to claim 7, characterized in that: A limiting block protrudes from the side wall of the insertion post. The side wall of the fixing groove is provided with a limiting groove to facilitate the insertion of the limiting block. The side wall of the fixing groove is also provided with an arc-shaped sliding groove that communicates with the limiting groove. The limiting block can slide in the sliding groove.
9. The stable high-throughput microsphere culture sheet according to claim 7, characterized in that: The counterweight plate contains a counterweight.
10. The stable high-throughput microsphere culture sheet according to claim 6, characterized in that: There are four counterweights, which are evenly distributed at the bottom of the culture plate body.