Production bacterium tank for cultivating methylobacterium

Abstract

The utility model provides a production fungus tank for methylobacterium cultivation, which belongs to the technical field of fungus cultivation and comprises a fungus tank body, a tank cover is rotatably connected to the top wall of the fungus tank body, feed pipes which are symmetrically distributed are fixedly connected to the top wall of the tank cover, a handle is fixedly connected to the top wall of the tank cover, and the handle is fixedly connected to the top wall of the fungus tank body. The outer wall of the fungus tank body is fixedly connected with a driving motor B, and the outer wall of the fungus tank body is fixedly connected with a discharge port. According to the utility model, the driving motor B is arranged to drive the extrusion hollow plate to rotate, so that the air outlet holes are periodically aligned with the ventilation cylinder, pulse type injection of mixed gas is realized, denser microbubbles can be formed, the contact area between the gas and thalli is increased, the dissolution of oxygen and methane is accelerated, and the mass transfer efficiency is remarkably improved; and in cooperation with the linkage design of the sealing frame and the conical spring, gas reverse flow is avoided, and the stability and controllability of the aeration process are ensured.

Description

Technical Field

[0001] This utility model relates to the field of fungal cultivation technology, and more specifically, to a production tank for cultivating Methylobacterium. Background Technology

[0002] Methylbacteria are Gram-negative bacteria with unique metabolic capabilities, capable of using one-carbon compounds such as methanol and methane as their sole carbon and energy sources for growth and reproduction. They demonstrate significant application value in biodegradation, biofuel synthesis, and plant growth promotion. Due to their metabolic characteristics, which depend on a strict gaseous environment (such as a precise ratio of methane to oxygen) and uniform nutrient distribution, large-scale cultivation using production tanks has become a key approach for industrial applications. Tank cultivation allows for precise control of parameters such as temperature, gas concentration, and stirring rate, ensuring the activity and metabolic efficiency of Methylbacteria and achieving high-density, high-yield cultivation.

[0003] However, existing Methylobacterium culture tanks have some problems: 1. In the aeration stage, traditional continuous aeration methods easily lead to uneven gas distribution and excessively large bubble size, resulting in low oxygen and methane dissolution efficiency, making it difficult to meet the special requirements of Methylobacterium for carbon source and dissolved oxygen; 2. In the detection stage, most tanks use fixed-point sensor arrangement, which cannot adapt to the concentration gradient changes caused by bacterial liquid stratification. There are errors in monitoring the methanol concentration and oxygen content of different liquid layers, making it difficult to achieve precise control of culture parameters, resulting in low bacterial growth efficiency and large fluctuations in the yield of metabolites.

[0004] Therefore, there is an urgent need for a production tank for cultivating Methylobacterium to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a production tank for cultivating Methylobacterium to solve the problems mentioned in the background art.

[0006] To achieve the above-mentioned objectives, this utility model provides the following technical solution:

[0007] A production tank for cultivating Methylobacterium, comprising a tank body, a tank lid rotatably connected to the top wall of the tank body, symmetrically distributed feed pipes fixedly connected to the top wall of the tank lid, a handle fixedly connected to the top wall of the tank lid, a drive motor B fixedly connected to the outer wall of the tank body, and a discharge port fixedly connected to the outer wall of the tank body, further comprising:

[0008] An airlift flow reaction assembly includes an inner bottom plate fixedly connected to the inner wall of the culture tank body, a uniformly distributed air exchange cylinder fixedly connected to the outer wall of the inner bottom plate, a sliding frame fixedly connected to the inner wall of the air exchange cylinder, a conical spring sleeved on the outer wall of the sliding frame, a sealing frame slidably connected to the outer wall of the sliding frame, and the outer wall of the sealing frame abutting against the inner wall of the air exchange cylinder, the sealing frame being fixedly connected to the conical spring, and a squeezing rod fixedly connected to the outer wall of the sealing frame;

[0009] A rotating gas conveying component is installed on the inner wall of the culture tank body, and the rotating gas conveying component cooperates with the air rise flow reaction component.

[0010] A reciprocating moving detection component is installed on the top wall of the culture tank body.

[0011] As a preferred technical solution of this application, the rotating gas conveying assembly includes a fixed sealing seat fixedly connected to the inner wall of the mushroom tank body, a rotating sealing disc rotatably connected to the outer wall of the fixed sealing seat, a compressed hollow plate fixedly connected to the outer wall of the rotating sealing disc, and the outer wall of the compressed hollow plate fixedly connected to the output end of the drive motor B. The top wall of the compressed hollow plate is provided with evenly distributed air outlet holes, the outer wall of the air outlet holes is fixedly connected with an anti-insertion plate, and the outer wall of the rotating sealing disc is provided with evenly distributed air inlet holes, and the air inlet holes are located on the inner wall of the fixed sealing seat.

[0012] As a preferred technical solution of this application, the reciprocating motion detection component includes a drive motor A fixedly connected to the top wall of the mushroom tank body. A reciprocating screw is fixedly connected to the output end of the drive motor A. A symmetrically distributed positioning plate is rotatably connected to the outer wall of the reciprocating screw, and the positioning plate is fixedly connected to the mushroom tank body. A symmetrically distributed guide rod is fixedly connected between the symmetrical positioning plates. An mounting plate is slidably connected to the outer wall of the guide rod, and the outer wall of the mounting plate abuts against the outer wall of the reciprocating screw. A methanol concentration sensor is fixedly connected to the outer wall of the mounting plate, and an oxygen sensor is also fixedly connected to the outer wall of the mounting plate.

[0013] As a preferred technical solution of this application, the outer wall of the mushroom tank is fixedly connected with symmetrically distributed support columns, and the outer wall of the support columns is fixedly connected with support plates.

[0014] As a preferred technical solution of this application, an L-shaped plate is fixedly connected to the outer wall of the mushroom tank body, a pump is fixedly connected to the outer wall of the L-shaped plate, an input pipe is fixedly connected to the pump outlet, and the input pipe is fixedly connected to the mushroom tank body and communicates with the fixed sealing seat.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] In the scheme of this application:

[0017] 1. The drive motor B drives the extrusion hollow plate to rotate, periodically aligning the air outlet with the air exchange cylinder to achieve pulsed injection of mixed gas. This forms denser microbubbles, increases the contact area between the gas and the bacteria, accelerates the dissolution of oxygen and methane, significantly improves mass transfer efficiency, and promotes the metabolic activity of Methylobacterium. Combined with the linkage design of the sealing frame and conical spring, backflow of gas is avoided, ensuring the stability and controllability of the aeration process. This solves the problem in the existing technology where the traditional continuous aeration method easily leads to uneven gas distribution and excessively large bubble size, resulting in low dissolution efficiency of oxygen and methane, which makes it difficult to meet the special requirements of Methylobacterium for carbon source and dissolved oxygen.

[0018] 2. The drive motor A rotates the reciprocating screw, causing the mounting plate to move up and down along the guide rod. This drives the methanol concentration sensor and oxygen sensor to monitor the parameters of different liquid layers in the culture tank in real time. Compared with traditional fixed-point detection, this design avoids measurement errors caused by stratification of bacterial concentration or local metabolic differences, ensuring that the data reflects the overall culture status. This solves the problem in the existing technology where most culture tanks use fixed-point sensor arrangements, which cannot adapt to the concentration gradient changes caused by bacterial stratification. This results in errors in monitoring the methanol concentration and oxygen content of different liquid layers, making it difficult to accurately control the culture parameters, leading to low bacterial growth efficiency and large fluctuations in the yield of metabolites. Attached Figure Description

[0019] Figure 1 A schematic diagram of the overall structure of the production tank for cultivating Methylobacteria provided in this application;

[0020] Figure 2 A schematic diagram of the internal structure of the production tank for cultivating Methylobacteria provided in this application;

[0021] Figure 3 A schematic diagram of the L-shaped plate portion of the production tank for cultivating Methylobacteria provided in this application;

[0022] Figure 4 A schematic diagram of the inner bottom plate of the production tank for cultivating Methylobacterium provided in this application;

[0023] Figure 5 A schematic diagram of the extruded hollow plate portion of the production tank for cultivating Methylobacteria provided in this application;

[0024] Figure 6 An exploded view of the rotating sealing disc portion of the production tank for cultivating Methylobacterium provided in this application;

[0025] Figure 7 This is a schematic diagram of the internal structure of the ventilation cylinder of the production tank for cultivating Methylobacterium provided in this application.

[0026] The image shows:

[0027] 1. Tank body; 2. Discharge port; 3. Support column; 4. Support plate; 5. Tank lid; 6. Handle; 7. Feed pipe; 8. Drive motor A; 9. Reciprocating screw; 10. Positioning plate; 11. Guide rod; 12. Mounting plate; 13. Methanol concentration sensor; 14. Oxygen sensor; 15. Drive motor B; 16. L-shaped plate; 17. Pump; 18. Input pipe; 19. Fixed sealing seat; 20. Inner bottom plate; 21. Rotating sealing disc; 22. Extruded hollow plate; 23. Air inlet; 24. Air outlet; 25. Anti-insertion plate; 26. Air exchanger; 27. Extrusion rod; 28. Sliding frame; 29. ​​Conical spring; 30. Sealing frame. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model.

[0029] like Figure 1-7 As shown, this embodiment proposes a production tank for cultivating Methylobacterium, including a tank body 1, a tank cover 5 rotatably connected to the top wall of the tank body 1, symmetrically distributed feed pipes 7 fixedly connected to the top wall of the tank cover 5, a handle 6 fixedly connected to the top wall of the tank cover 5, a drive motor B15 fixedly connected to the outer wall of the tank body 1, and a discharge port 2 fixedly connected to the outer wall of the tank body 1. After cultivation, the bacterial solution is discharged through the discharge port 2. The tank also includes:

[0030] The airflow reaction assembly includes an inner bottom plate 20 fixedly connected to the inner wall of the culture tank body 1. Uniformly distributed ventilation cylinders 26 are fixedly connected to the outer wall of the inner bottom plate 20. A sliding frame 28 is fixedly connected to the inner wall of the ventilation cylinders 26. A conical spring 29 is sleeved on the outer wall of the sliding frame 28. A sealing frame 30 is slidably connected to the outer wall of the sliding frame 28, and the outer wall of the sealing frame 30 abuts against the inner wall of the ventilation cylinders 26. The sealing frame 30 is fixedly connected to the conical spring 29. A compression rod 2 is fixedly connected to the outer wall of the sealing frame 30. 7. During the rotation of the extrusion hollow plate 22, the sealing frame 30 is pushed upward, compressing the conical spring 29. Gas is released from the top of the air exchange cylinder 26, forming microbubbles. As the extrusion hollow plate 22 continues to rotate, the air outlet 24 is misaligned. The conical spring 29 rebounds and pushes the sealing frame 30 downward to close the air exchange cylinder 26, realizing pulse aeration. The fixed sealing seat 19 and the rotating sealing disc 21 form a dynamic seal, ensuring that gas is only directionally input through the air inlet 23. The anti-insertion plate 25 prevents bacteria or impurities from clogging the air outlet 24.

[0031] A rotating gas conveying component is installed on the inner wall of the culture tank body 1, and the rotating gas conveying component cooperates with the air rise flow reaction component.

[0032] A reciprocating moving detection component is installed on the top wall of the main body 1 of the culture tank.

[0033] like Figure 5-6 As shown, in a preferred embodiment, based on the above method, the rotating gas conveying assembly further includes a fixed sealing seat 19 fixedly connected to the inner wall of the mushroom tank body 1. A rotating sealing disc 21 is rotatably connected to the outer wall of the fixed sealing seat 19. A compressed hollow plate 22 is fixedly connected to the outer wall of the rotating sealing disc 21, and the outer wall of the compressed hollow plate 22 is fixedly connected to the output end of the drive motor B15. The top wall of the compressed hollow plate 22 has evenly distributed air outlet holes 24, and the outer wall of the air outlet holes 24 is fixedly connected to... The rotating sealing disc 21 is equipped with an anti-insertion plate 25. The outer wall of the rotating sealing disc 21 has evenly distributed air inlet holes 23, which are located on the inner wall of the fixed sealing seat 19. The drive motor B15 drives the extrusion hollow plate 22 to rotate. The air outlet hole 24 on the extrusion hollow plate 22 periodically aligns with the air exchange cylinder 26. When the air outlet hole 24 coincides with the air exchange cylinder 26, the mixed gas (oxygen and methanol gas) input by the pump 17 enters the extrusion hollow plate 22 through the air inlet hole 23 and is sprayed into the air exchange cylinder 26 from the air outlet hole 24.

[0034] like Figure 2 and Figure 4 As shown, in a preferred embodiment, based on the above method, the reciprocating moving detection component further includes a drive motor A8 fixedly connected to the top wall of the culture tank body 1. A reciprocating screw 9 is fixedly connected to the output end of the drive motor A8. A symmetrically distributed positioning plate 10 is rotatably connected to the outer wall of the reciprocating screw 9, and the positioning plate 10 is fixedly connected to the culture tank body 1. A symmetrically distributed guide rod 11 is fixedly connected between the symmetrical positioning plates 10. An mounting plate 12 is slidably connected to the outer wall of the guide rod 11, and the outer wall of the mounting plate 12 abuts against the outer wall of the reciprocating screw 9. A methanol concentration sensor 13 is fixedly connected to the outer wall of the mounting plate 12, and an oxygen sensor 14 is also fixedly connected to the outer wall of the mounting plate 12. The drive motor A8 drives the reciprocating screw 9 to rotate, causing the mounting plate 12 to move up and down along the guide rod 11. The methanol concentration sensor 13 and the oxygen sensor 14 dynamically monitor the parameters of different liquid layers with the mounting plate 12, avoiding local measurement errors.

[0035] like Figure 1 As shown, in a preferred embodiment, based on the above method, the outer wall of the mushroom tank body 1 is further provided with symmetrically distributed support columns 3, and the outer wall of the support columns 3 is provided with support plates 4. The equipment is supported and fixed by the support columns 3 and the support plates 4.

[0036] like Figure 3As shown, in a preferred embodiment, based on the above method, an L-shaped plate 16 is fixedly connected to the outer wall of the mushroom tank body 1, a pump 17 is fixedly connected to the outer wall of the L-shaped plate 16, an input pipe 18 is fixedly connected to the outlet of the pump 17, and the input pipe 18 is fixedly connected to the mushroom tank body 1. The input pipe 18 is connected to the fixed sealing seat 19. The mixed gas (oxygen and methanol gas) input by the pump 17 enters the extrusion hollow plate 22 through the air inlet 23 and is sprayed into the air exchange cylinder 26 from the air outlet 24.

[0037] Specifically, in use, the production tank for Bacillus thuringiensis cultivation works as follows: the drive motor B15 rotates the extrusion hollow plate 22. The air outlet 24 on the extrusion hollow plate 22 periodically aligns with the ventilation cylinder 26. When the air outlet 24 coincides with the ventilation cylinder 26, the mixed gas (oxygen and methanol gas) input by the pump 17 enters the extrusion hollow plate 22 through the air inlet 23 and is sprayed into the ventilation cylinder 26 from the air outlet 24. During the rotation of the extrusion hollow plate 22, it squeezes and pushes the sealing frame 30 upward, compressing the conical spring 29. The gas is released from the top of the ventilation cylinder 26, forming microbubbles. As the extrusion hollow plate 22 continues to rotate, the air outlet 24 is misaligned, and the conical spring... Spring 29 rebounds and pushes sealing frame 30 down to close ventilation cylinder 26, realizing pulse aeration. Fixed sealing seat 19 and rotating sealing disc 21 form a dynamic seal to ensure that gas is only directionally input through air inlet 23. Anti-insertion plate 25 prevents bacteria or impurities from blocking air outlet 24. Drive motor A8 drives reciprocating screw 9 to rotate, causing mounting plate 12 to move up and down along guide rod 11. Methanol concentration sensor 13 and oxygen sensor 14 dynamically monitor parameters of different liquid layers with mounting plate 12 to avoid local measurement errors. After cultivation, bacterial liquid is discharged through discharge port 2. Feed pipe 7 is used to add culture medium or regulator, and handle 6 facilitates opening and closing of tank lid 5.

[0038] The above embodiments are only used to illustrate the present utility model and are not intended to limit the technical solutions described in the present utility model. Although the present utility model has been described in detail with reference to the above embodiments, the present utility model is not limited to the specific embodiments described above. Therefore, any modifications or equivalent substitutions to the present utility model, and all technical solutions and improvements that do not depart from the spirit and scope of the invention, are covered within the scope of the claims of the present utility model.

Claims

1. A production tank for cultivating Methylobacterium, comprising a tank body (1), characterized in that, The top wall of the mushroom tank body (1) is rotatably connected to a tank cover (5), the top wall of the tank cover (5) is fixedly connected to symmetrically distributed feed pipes (7), the top wall of the tank cover (5) is fixedly connected to a handle (6), the outer wall of the mushroom tank body (1) is fixedly connected to a drive motor B (15), the outer wall of the mushroom tank body (1) is fixedly connected to a discharge port (2), and also includes: An airlift flow reaction assembly includes an inner bottom plate (20) fixedly connected to the inner wall of the culture tank body (1), a uniformly distributed air exchange cylinder (26) fixedly connected to the outer wall of the inner bottom plate (20), a sliding frame (28) fixedly connected to the inner wall of the air exchange cylinder (26), a conical spring (29) sleeved on the outer wall of the sliding frame (28), a sealing frame (30) slidably connected to the outer wall of the sliding frame (28), and the outer wall of the sealing frame (30) abutting against the inner wall of the air exchange cylinder (26), the sealing frame (30) being fixedly connected to the conical spring (29), and a squeezing rod (27) fixedly connected to the outer wall of the sealing frame (30). A rotating gas conveying component is installed on the inner wall of the mushroom tank body (1), and the rotating gas conveying component cooperates with the gas rise flow reaction component. A reciprocating moving detection component is installed on the top wall of the main body (1) of the culture tank.

2. The production tank for cultivating Methylobacterium according to claim 1, characterized in that, The rotating gas conveying assembly includes a fixed sealing seat (19) fixedly connected to the inner wall of the mushroom tank body (1). A rotating sealing disc (21) is rotatably connected to the outer wall of the fixed sealing seat (19). A compressed hollow plate (22) is fixedly connected to the outer wall of the rotating sealing disc (21). The outer wall of the compressed hollow plate (22) is fixedly connected to the output end of the drive motor B (15). The top wall of the compressed hollow plate (22) is provided with evenly distributed air outlet holes (24). An anti-insertion plate (25) is fixedly connected to the outer wall of the air outlet holes (24). The outer wall of the rotating sealing disc (21) is provided with evenly distributed air inlet holes (23). The air inlet holes (23) are located on the inner wall of the fixed sealing seat (19).

3. The production tank for cultivating Methylobacterium according to claim 1, characterized in that, The reciprocating motion detection component includes a drive motor A (8) fixedly connected to the top wall of the mushroom tank body (1). A reciprocating screw (9) is fixedly connected to the output end of the drive motor A (8). A symmetrically distributed positioning plate (10) is rotatably connected to the outer wall of the reciprocating screw (9). The positioning plate (10) is fixedly connected to the mushroom tank body (1). A symmetrically distributed guide rod (11) is fixedly connected between the symmetrical positioning plates (10). An installation plate (12) is slidably connected to the outer wall of the guide rod (11). The outer wall of the installation plate (12) abuts against the outer wall of the reciprocating screw (9). A methanol concentration sensor (13) is fixedly connected to the outer wall of the installation plate (12). An oxygen sensor (14) is also fixedly connected to the outer wall of the installation plate (12).

4. The production tank for cultivating Methylobacterium according to claim 1, characterized in that, The outer wall of the mushroom tank body (1) is fixedly connected with symmetrically distributed support columns (3), and the outer wall of the support columns (3) is fixedly connected with support plates (4).

5. The production tank for cultivating Methylobacterium according to claim 1, characterized in that, An L-shaped plate (16) is fixedly connected to the outer wall of the mushroom tank body (1). A pump (17) is fixedly connected to the outer wall of the L-shaped plate (16). An input pipe (18) is fixedly connected to the outlet of the pump (17). The input pipe (18) is fixedly connected to the mushroom tank body (1). The input pipe (18) is connected to the fixed sealing seat (19).

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