Immobilized enzyme reaction device

Abstract

The utility model discloses an immobilized enzyme reaction device which comprises an adjusting assembly used for changing the position of an immobilized enzyme catalysis assembly, the adjusting assembly comprises a telescopic rod and a shaft block connected with the immobilized enzyme catalysis assembly, and the shaft block is connected to the telescopic end of the telescopic rod. Through the synergistic effect of the electric telescopic rod and the adjustable movable buckle assembly, rapid lifting and position adjustment of the immobilized enzyme screen plate are achieved.

Description

Technical Field

[0001] This utility model belongs to the field of enzyme chemical engineering, and specifically relates to an immobilized enzyme reaction device. Background Technology

[0002] Immobilized enzymes are a new technology developed and gradually applied in the 1960s. They are enzymes that catalyze reactions within a certain spatial range and can be used repeatedly and continuously. Normally, enzyme catalytic reactions take place in solution, while immobilized enzymes are water-soluble enzymes that have been treated by physical or chemical methods to make them insoluble in water but still retain their enzymatic activity. Immobilized enzymes have advantages such as high stability, easy separation, reusability, and continuous and controllable operation, and therefore have broad application prospects in industrial production, chemical analysis, medicine and other fields.

[0003] In existing immobilized enzyme reaction devices, the immobilized enzyme is directly added to the reaction solution during operation. However, the amount of immobilized enzyme cannot be flexibly adjusted for different reaction solutions, such as different concentrations, types, and mixing ratios. This results in poor adaptability of the immobilized enzyme reaction device, a limited range of practical applications, and low practicality. Utility Model Content

[0004] The present invention aims to at least partially solve one of the technical problems existing in the prior art. To this end, the present invention provides an immobilized enzyme reaction device.

[0005] In a first aspect, this invention provides an immobilized enzyme reaction apparatus, comprising: an adjustment component for changing the position of an immobilized enzyme catalytic component, the adjustment component including a telescopic rod and a shaft block connected to the immobilized enzyme catalytic component, the shaft block being connected to the telescopic end of the telescopic rod.

[0006] Preferably, the adjustment component further includes an adjustable movable snap-fit ​​component, and the immobilized enzyme catalytic component is slidably connected to the shaft block through the adjustable movable snap-fit ​​component.

[0007] Preferably, the adjustable movable snap-fit ​​assembly includes: a first T-shaped snap-fit ​​groove disposed on the shaft block; and a fixing block slidably connected to the immobilized enzyme catalytic assembly, wherein the fixing block is provided with a first T-shaped snap-fit ​​slider that matches the shape of the first T-shaped snap-fit ​​groove, and the first T-shaped snap-fit ​​slider can slide along the first T-shaped snap-fit ​​groove.

[0008] Preferably, the adjustable movable buckle assembly further includes: fixed slots disposed on both sides of the shaft block; and fixed clamp legs clamping both sides of the fixed block, wherein the fixed clamp legs are provided with top blocks that match the shape of the fixed slots, and the bottom end of the fixed clamp legs is provided with a second T-shaped insert slider, the second T-shaped insert slider matching the shape of a second T-shaped insert groove disposed on the immobilized enzyme catalytic assembly, and the second T-shaped insert slider can slide along the second T-shaped insert groove.

[0009] Preferably, the direction in which the first T-shaped insert slider slides along the first T-shaped insert groove is perpendicular to the direction in which the second T-shaped insert slider slides along the second T-shaped insert groove.

[0010] Preferably, the fixing clip legs are clamped to both sides of the fixing block by screws.

[0011] Preferably, the telescopic rod is an electric telescopic rod, and the bottom of the electric telescopic rod is provided with a base.

[0012] Preferably, the immobilized enzyme reaction device further includes: a reaction chamber and a stirring assembly disposed in the inner cavity of the reaction chamber, wherein embedded grooves are provided on both sides of the inner cavity of the reaction chamber, and the stirring assembly includes a waterproof drive motor embedded in the embedded groove and stirring blades installed at the output end of the waterproof drive motor.

[0013] Preferably, a sealing cover plate is hinged at the top opening of the reaction chamber, and the sealing cover plate and the two sides of the edge of the reaction chamber are provided with fitting grooves that are adapted to the two ends of the shaft block. A feed sealing pipe is connected to the sealing cover plate.

[0014] Preferably, a drain pipe is connected to the front end of the reaction tank, and a ball valve switch is installed on the drain pipe.

[0015] This invention achieves rapid lifting and positioning adjustment of the immobilized enzyme grid plate through the synergistic action of an electric telescopic rod and an adjustable movable buckle assembly. The number of enzyme grid plates can be freely increased or decreased according to reaction requirements, adapting to different concentrations or types of reaction solutions, significantly improving the applicability of the device. This invention employs a T-shaped clip-slider-groove system (first T-shape / second T-shape) combined with a fixed slot design to ensure dual positioning of the enzyme grid plate in both horizontal and vertical directions. The screw-operated locking mechanism of the screw and the mounting slot further enhances the vibration resistance of the immobilized enzyme grid plate during the reaction process. In addition, this invention integrates an electric telescopic rod drive system to achieve fully automatic lifting control of the enzyme grid plate assembly. Combined with a waterproof drive motor-driven stirring assembly, this forms a closed-loop reaction control system, effectively improving reaction efficiency. Through the precise fit between the sealing cover and the fitting groove, combined with the independent channel design of the inlet sealing pipe and the drain pipe, a completely closed reaction environment is constructed, avoiding cross-contamination. The second T-shaped slide rail system allows for quick disassembly of the fixing clip legs, significantly reducing cleaning and maintenance costs. Furthermore, the three-dimensional layout of the embedded shaft blocks allows for the vertical stacking of up to dozens of enzyme mesh plates in a standard reaction chamber, increasing the effective contact area compared to traditional flat-plate reactors. These innovative designs give the device a significant competitive advantage in fields such as biopharmaceuticals, food processing, and environmental remediation, and it is particularly suitable for pilot-scale applications requiring precise control of enzymatic reactions. Attached Figure Description

[0016] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0017] Figure 1 This is a schematic diagram of the overall appearance structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the immobilized enzyme reaction device of this utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the immobilized enzyme mesh plate of this utility model after fixation.

[0020] Figure 4 This is a schematic diagram of the structure of the immobilized enzyme mesh plate before fixation according to this utility model;

[0021] Figure 5 This is a schematic diagram of the fixed clip leg structure of this utility model;

[0022] Figure 6 This is a schematic diagram showing the connection between the shaft block and the electric telescopic rod of this utility model;

[0023] Figure 7This is a structural diagram of the stirring assembly and bonding groove of this utility model.

[0024] In the diagram, 1. Reaction chamber; 2. Adjustment assembly; 3. Electric telescopic rod; 4. (Embedded) shaft block; 5. Immobilized enzyme mesh plate; 6. Adjustable movable buckle assembly; 7. Stirring assembly; 8. Sealing cover plate; 9. Fixed clamp legs; 10. Fixed block; 11. First T-shaped embedded slider; 12. Support rod; 13. Tightening screw; 14. Top block; 15. First T-shaped embedded groove; 16. Second T-shaped embedded groove; 17. Fixed slot; 18. Base; 19. Embedding groove; 20. Waterproof drive motor; 21. Stirring blade; 22. Adhesion groove; 23. Feed sealing pipe; 24. Drain pipe; 25. Ball valve switch; 26. Mounting rotating groove; 27. Second T-shaped embedded slider. Detailed Implementation

[0025] The following embodiments are provided to enable those skilled in the art to better understand the present invention. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by the present invention, but are merely illustrative examples. Unless otherwise specified, the raw materials, reagents, or devices mentioned in the following embodiments are commercially available or obtained through known existing methods.

[0026] like Figures 1-7 As shown, the immobilized enzyme reaction apparatus provided in this embodiment includes a reaction chamber 1, an adjustment component 2 installed in the reaction chamber 1, the adjustment component 2 including two electric telescopic rods 3, a (clamping) shaft block 4 connected between the two electric telescopic rods 3, a plurality of immobilized enzyme mesh plates 5 sequentially installed below the (clamping) shaft block 4, and an adjustable movable latching component 6 disposed between the (clamping) shaft block 4 and the immobilized enzyme mesh plates 5. Stirring components 7 are installed on both sides of the inner cavity of the reaction chamber 1, and a sealing cover plate 8 is hinged to the top opening of the reaction chamber 1. The reaction chamber 1 is used for temporary storage of immobilized enzymes during the reaction with the solution to be reacted; the adjustment component 2 can lift the immobilized enzyme fixing frame out of the reaction chamber 1, allowing for flexible adjustment of the amount of immobilized enzyme when facing different reaction solutions; two electric telescopic rods 3 are used to realize the automatic lifting and lowering of the (embedded) shaft block 4; the (embedded) shaft block 4 is used to install and support several immobilized enzyme mesh plates 5, and also facilitates the connection of the telescopic ends of the two electric telescopic rods 3 together; the immobilized enzyme mesh plate 5 contains several reactive enzymes, and is used to react with the solution to be reacted, while the immobilized enzyme mesh plate 5 can be reused repeatedly; the adjustable movable buckle component 6 is used to adjustably slide and fix several immobilized enzyme mesh plates 5 under the (embedded) shaft block 4; the stirring component 7 is used to stir the reaction solution in the reaction chamber 1, thereby making the reaction more complete; the sealing cover 8 is used to seal the opening at the top of the reaction chamber 1, and can also be opened to rinse the interior of the reaction chamber 1 after operation.

[0027] Furthermore, such as Figures 2-4 As shown, the adjustable movable buckle assembly 6 includes fixed clamp legs 9 symmetrically sliding and snapping into the top surface of the immobilized enzyme mesh plate 5, a fixing block 10 disposed between the two fixed clamp legs 9, and a first T-shaped snapping slider 11 disposed at the top of the fixing block 10; the two fixed clamp legs 9 are used to snap and hold the immobilized enzyme mesh plate 5 onto the (snap) shaft block 4, and the fixing block 10 is used to fix the first T-shaped snapping slider 11 to the immobilized enzyme mesh plate 5 together. The first T-shaped snapping slider 11 and the first T-shaped snapping groove 15 cooperate with each other to slide and snap the immobilized enzyme mesh plate 5 onto the (snap) shaft block 4.

[0028] Furthermore, such as Figure 4 and Figure 5 As shown, the fixing clip leg 9 includes a support rod 12, a screw 13 rotatably installed in the support rod 12, a second T-shaped locking slider 27 provided on the bottom end face of the support rod 12, and a top block 14 provided on the inner side of the top end of the support rod 12. The fixing block 10 has mounting grooves 26 on both sides that are screwed to fit the screw 13. The support rod 12 is used to connect the second T-shaped locking slider 27 and the top block 14 together. The second T-shaped locking slider 27 and the second T-shaped locking groove 16 cooperate with each other to allow the support rod 12 to slide on the immobilized enzyme mesh plate 5. The screw 13 and the mounting groove 26 cooperate with each other to fix the support rod 12 on the fixing block 10 so as to position and lock the immobilized enzyme mesh plate 5 that is slidably locked on the shaft block 4. The top block 14 and the fixing groove 17 cooperate with each other to lock the immobilized enzyme mesh plate 5 on the shaft block 4 more firmly.

[0029] Furthermore, such as Figure 3 , Figure 4 as well as Figure 6 As shown, the bottom surface of the (embedded) shaft block 4 is provided with a first T-shaped embedding groove 15 that is adapted to the first T-shaped embedding slider 11, and the top surface of the immobilized enzyme mesh plate 5 is provided with a second T-shaped embedding groove 16 that is adapted to the second T-shaped embedding slider 27; the first T-shaped embedding slider 11 and the first T-shaped embedding groove 15 cooperate with each other to slide and embed the immobilized enzyme mesh plate 5 onto the (embedded) shaft block 4, and the second T-shaped embedding slider 27 and the second T-shaped embedding groove 16 cooperate with each other to slide the support rod 12 onto the immobilized enzyme mesh plate 5.

[0030] Furthermore, such as Figure 2 and Figure 6As shown, the (embedded) shaft block 4 has fixed slots 17 on both sides that are adapted to the top block 14. The electric telescopic rod 3 has a base 18 at the bottom. The base 18 is fixedly installed on the side of the reaction chamber 1 by bolts. The top block 14 and the fixed slots 17 cooperate with each other to make it easier to position and lock the immobilized enzyme mesh plate 5 on the (embedded) shaft block 4 more firmly. The base 18 is used to support and fix the electric telescopic rod 3, and at the same time, it is easy to fix the electric telescopic rod 3 on the side of the reaction chamber 1.

[0031] Furthermore, such as Figure 7 As shown, the reaction chamber 1 has embedded grooves 19 on both sides of the inner cavity. The stirring assembly 7 includes a waterproof drive motor 20 embedded in the embedded groove 19 and a stirring blade 21 installed at the output end of the waterproof drive motor 20. The embedded groove 19 is used to fix the waterproof drive motor 20, and the waterproof drive motor 20 is used to provide driving force for the rotation of the stirring blade 21. The stirring blade 21 is used to stir the reaction solvent under the drive of the waterproof drive motor 20, so that the reaction is more complete.

[0032] Furthermore, such as Figure 1 and Figure 7 As shown, both sides of the sealing cover plate 8 and the reaction chamber 1 are provided with fitting grooves 22 that are adapted to the two ends of the shaft block 4. The sealing cover plate 8 is connected to the feed sealing pipe 23. The fitting grooves 22 enable the sealing cover plate 8 to better seal the reaction chamber 1. The feed sealing pipe 23 is used to rotate open to inject the solution to be reacted into the reaction chamber 1, and then rotate it closed after injection.

[0033] Furthermore, such as Figure 1 and Figure 7 As shown, a drain pipe 24 is connected to the front end of the reaction tank 1, and a ball valve switch 25 is installed on the drain pipe 24; the drain pipe 24 is used to drain the solution after the reaction in the reaction tank 1, and the ball valve switch 25 is used to control the opening and closing of the drain pipe 24.

[0034] like Figures 1-7 As shown, the principle of the immobilized enzyme reaction device provided in this embodiment is as follows: When using the device, first open the sealing cover 8 hinged at the top opening of the reaction chamber 1, extend the two electric telescopic rods 3 so that the (embedded) shaft block 4 rises out of the reaction chamber 1 to the designated position, then select a certain amount of immobilized enzyme mesh plate 5 according to the type of immobilized enzyme required for the reaction solution, and then fix the immobilized enzyme mesh plate 5 onto the (embedded) shaft block 4 through the adjustable movable buckle assembly 6.

[0035] When installing the immobilized enzyme mesh plate 5, the first T-shaped clip slider 11 should be clipped into the first T-shaped clip groove 15 on the (clamping) shaft block 4. Then, the immobilized enzyme mesh plate 5 should be installed on the (clamping) shaft block 4 and slid to a suitable position. Then, the second T-shaped clip slider 27 and the second T-shaped clip groove 16 should cooperate with each other so that the top blocks 14 on the inner side of the top of the two support rods 12 are clamped in the fixing groove 17. Rotate the screw 13 in the support rod 12 to screw it into the installation rotating groove 26 so that the fixing clip legs 9 are fixed on the fixing block 10.

[0036] After the immobilized enzyme mesh plate 5 is installed, the two electric telescopic rods 3 retract to their initial state, so that the (embedded) shaft block 4 with several immobilized enzyme mesh plates 5 also returns to its initial position in the reaction chamber 1, and then the sealing cover plate 8 at the top opening of the reaction chamber 1 is closed.

[0037] Unscrew the feed sealing pipe 23 and input the solution to be reacted into the reaction chamber 1 through the feed sealing pipe 23. After the injection is completed, close the feed sealing pipe 23. The solution to be reacted in the reaction chamber 1 will react rapidly with several immobilized enzyme mesh plates 5. At this time, the waterproof drive motor 20 starts to work and rotates the stirring blade 21 to make the reaction between the solution to be reacted and the immobilized enzyme mesh plates 5 faster and more complete.

[0038] After the reaction between the solution to be reacted and the immobilized enzyme mesh plate 5 is completed, open the ball valve switch 25 to allow the reacted solution to flow out from the drain pipe 24. After the solution has flowed out, close the ball valve switch 25. If the solution does not need to be replaced, the solution to be reacted can be injected back into the feed sealing pipe 23 through the feed sealing pipe 23. If the reaction solution needs to be replaced, first open the sealing cover plate 8, extend the two electric telescopic rods 3 to raise the (embedded) shaft block 4 from the reaction chamber 1 to the designated position, replace the immobilized enzyme mesh plate 5 according to the solution to be reacted, and install it on the (embedded) shaft block 4 through the adjustable movable buckle assembly 6. Retract the two electric telescopic rods 3 to the initial state so that the (embedded) shaft block 4 retracts to the initial state, and then close the sealing cover plate 8.

[0039] This utility model may have other embodiments. All technical solutions formed by equivalent substitution or equivalent transformation fall within the scope of protection claimed by this utility model.

Claims

1. An immobilized enzyme reaction apparatus, characterized in that, include: An adjustment component for changing the position of an immobilized enzyme catalytic component, the adjustment component including a telescopic rod and a shaft block connected to the immobilized enzyme catalytic component, the shaft block being connected to the telescopic end of the telescopic rod; The adjustment component includes an adjustable movable snap-fit ​​component, and the immobilized enzyme catalysis component is slidably connected to the shaft block via the adjustable movable snap-fit ​​component; The adjustable movable buckle assembly includes: A first T-shaped locking groove is provided on the shaft block; and A fixing block slidably connected to the immobilized enzyme catalytic component, wherein the fixing block is provided with a first T-shaped locking slider that matches the shape of the first T-shaped locking groove, and the first T-shaped locking slider can slide along the first T-shaped locking groove.

2. The immobilized enzyme reaction apparatus according to claim 1, characterized in that, The immobilized enzyme catalytic component is an immobilized enzyme mesh plate.

3. The immobilized enzyme reaction apparatus according to claim 1, characterized in that, The adjustable movable buckle assembly also includes: Fixing slots are provided on both sides of the shaft block; and The fixing clip legs are clamped on both sides of the fixing block. Each fixing clip leg is provided with a top block that matches the shape of the fixing slot. The fixing clip legs are also provided with a second T-shaped insert slider. The second T-shaped insert slider matches the shape of a second T-shaped insert groove provided on the immobilized enzyme catalytic component. The second T-shaped insert slider can slide along the second T-shaped insert groove.

4. The immobilized enzyme reaction apparatus according to claim 3, characterized in that, The direction in which the first T-shaped insert slider slides along the first T-shaped insert groove is perpendicular to the direction in which the second T-shaped insert slider slides along the second T-shaped insert groove.

5. The immobilized enzyme reaction apparatus according to claim 3, characterized in that, The fixing clip legs are clamped to both sides of the fixing block by screws.

6. The immobilized enzyme reaction apparatus according to claim 3, characterized in that, The telescopic pole is an electric telescopic pole, and a base is provided at the bottom of the electric telescopic pole.

7. The immobilized enzyme reaction apparatus according to claim 6, characterized in that, The immobilized enzyme reaction apparatus further includes: The reaction chamber and the stirring assembly disposed in the inner cavity of the reaction chamber are provided with embedding grooves on both sides of the inner cavity of the reaction chamber. The stirring assembly includes a waterproof drive motor embedded in the embedding groove and stirring blades installed at the output end of the waterproof drive motor.

8. The immobilized enzyme reaction apparatus according to claim 7, characterized in that, A sealing cover plate is hinged at the top opening of the reaction chamber. The sealing cover plate and both sides of the edge of the reaction chamber are provided with fitting grooves that are adapted to the two ends of the shaft block. A feed sealing pipe is connected to the sealing cover plate.

9. The immobilized enzyme reaction apparatus according to claim 7, characterized in that, A drain pipe is connected to the front end of the reaction chamber, and a ball valve switch is installed on the drain pipe.

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