Electric thin-layer chromatography sample applicator

Abstract

The utility model discloses an electric thin-layer chromatography sample applicator, which relates to the technical field of analytical instruments and comprises a heating sample applicator, the upper surface of the heating sample applicator is fixedly connected with a supporting seat, the inner side wall of the supporting seat is fixedly connected with an auxiliary rod, the outer side wall of the supporting seat is fixedly connected with a motor, and the inner wall of the supporting seat is rotatably connected with a threaded rod. An output shaft of the motor penetrates through one end of the supporting seat to be fixedly connected with a threaded rod, the outer surface of the threaded rod is in threaded connection with a sample application arm, and the sample application arm is connected with a first electric push rod which is connected with a sample application nozzle. According to the utility model, a plurality of parallel strips can be formed by repeated sample application, an average value of detection results corresponding to the strips can be taken subsequently to reduce errors and improve the detection accuracy, and the heating temperature of the heater to the thin-layer plate can be adjusted through the controller, so that the performance of components of the same sample at different temperatures can be analyzed.

Description

Technical Field

[0001] This utility model relates to the field of analytical instrument technology, and in particular to an electric thin-layer chromatography spotter. Background Technology

[0002] An electric thin-layer chromatography spotter is a specialized instrument used in thin-layer chromatography analysis to precisely apply samples onto a thin-layer plate. In organic chemistry, it is used for qualitative and quantitative analysis of organic compounds, such as analyzing the purity of components in synthetic products and identifying the structure of unknown organic compounds. In inorganic chemistry, it can be used to detect the components and determine the content of substances such as metal ion complexes and inorganic acid salts. The analysis is aided by separation and color development operations after spotting in thin-layer chromatography.

[0003] The utility model patent with patent number CN220932889U discloses a thin-layer spotting auxiliary device for the testing of traditional Chinese medicine materials. By setting a base plate to fix the thin-layer chromatographic plate, and setting a guide rod, a first movable rod and a sliding frame to restrict the movement trajectory of the micro-injector or capillary under manual spotting, the accuracy of manual spotting is improved. By setting a sliding strip and a clamping block in the through hole and cooperating with a second spring, the spotting device can be easily clamped and assembled on the sliding frame, so that the drop height of the sample to be tested is fixed and the uniformity of the spot or line spot is guaranteed.

[0004] However, in the above-mentioned patented sampling process, sampling can only be carried out in a limited number of positions. It is not possible to move vertically to sample multiple rows for subsequent comparative analysis. Furthermore, the thin-layer plate itself has a certain area available for sample sampling. If vertical movement is not possible, it not only wastes the thin-layer plate, but if manual movement is used, it is difficult to ensure that the distance of each movement is accurate and consistent, and it is also difficult to accurately control the position of each row of sampling, which can easily lead to problems such as sampling position deviation and uneven spacing.

[0005] Therefore, an electric thin-layer chromatography spotter is proposed. Summary of the Invention

[0006] To overcome the problems mentioned in the background art, the technical implementation scheme of this utility model is as follows:

[0007] An electric thin-layer chromatography spotter includes a heated spotter, a support base fixedly connected to the upper surface of the heated spotter, an auxiliary rod fixedly connected to the inner side wall of the support base, a motor fixedly connected to the outer side wall of the support base, a threaded rod rotatably connected to the inner wall of the support base, an output shaft of the motor passing through one end of the support base and fixedly connected to the threaded rod, a spotting arm threadedly connected to the outer surface of the threaded rod, a sliding plate slidably connected to the spotting arm, an electric push rod fixedly connected to one end of the sliding plate, a limit telescopic rod fixedly connected to the other end of the sliding plate, a support frame fixedly connected to the telescopic end of the electric push rod, a spotting nozzle fixedly connected to the lower part of the support frame, an air pump and an air-liquid separator respectively installed on one side of the heated spotter, the air outlet of the air pump connected to the air-liquid separator, and the air outlet of the air-liquid separator connected to the spotting nozzle through an air pipe.

[0008] Preferably, an extension plate is fixedly connected to the upper surface of the support frame, and an electric push rod is fixedly connected to the extension plate.

[0009] Preferably, the end of the sampling arm near the lower part is slidably connected to the auxiliary rod, and the telescopic end of the limiting telescopic rod is fixedly connected to the outer surface of the support frame.

[0010] Preferably, the upper surface of the support frame is fixedly connected to two protrusions, each of which is slidably connected to a sliding post. The outer surface of each of the two sliding posts is fitted with a support spring. One end of each sliding post that passes through the inner wall of the protrusion is fixedly connected to a bent rod, and the ends of the two bent rods that are close to each other are fixedly connected to a clamping sleeve.

[0011] Preferably, the two clamping sleeves are arc-shaped, one end of the support spring is fixed to the end of the sliding column away from the support frame, and the other end of the support spring is fixedly connected to the protrusion on the upper surface of the support frame.

[0012] Preferably, a threaded post is threadedly connected to one side wall of the sliding plate near the electric push rod, and a compression pad is fixedly connected to one end of the threaded post that passes through the sliding plate. The compression pad is made of rubber, and the inner wall of the sampling arm is frosted.

[0013] Preferably, the sampling arm is hollow inside, and the outer surface is provided with a groove for the sliding plate to slide. One end of the sliding plate is located in the groove, and the other end extends out of the groove, and the part extending out of the groove is U-shaped.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] For precise quantitative analysis of components in a sample, multiple spottings can be repeated to form multiple parallel bands. The average value of the detection results corresponding to these bands can then be taken to reduce errors and improve detection accuracy. The heating temperature of the thin-layer plate can also be adjusted by the controller to analyze the performance of the same sample components at different temperatures. A band can be formed by spotting the sample on the same thin-layer plate first, and then a new band can be formed by moving the plate and spotting the sample again. Then, each band can be developed and analyzed under different temperature conditions without the need to manually move the thin-layer plate, thus achieving multiple spottings.

[0016] By uniformly spreading the sample on a thin-layer plate to form a strip, the concentration of the strip is relatively high after spotting, and the sample distribution is more regular and uniform. When combined with subsequent quantitative analysis methods such as optical density scanning, the content of each component can be determined more accurately, which helps to improve the accuracy of quantitative analysis.

[0017] Water vapor separation ensures the stability of sample concentration, making the sample spotting volume more precise and controllable, which is beneficial for accurate analysis. The dry gas after water vapor separation allows the sample to be more regularly distributed on the thin-layer plate, which helps to improve the separation resolution, better observe and analyze the band characteristics of each component, and avoid water vapor contamination which can change the physical properties of the sample, causing the bands formed on the thin-layer plate to diffuse or become irregular in shape.

[0018] For samples with higher viscosity, the distance needs to be increased appropriately to allow the atomized droplets more space to disperse and prevent them from sticking together. For samples that are thinner and more easily diffused, the distance needs to be reduced to prevent the sample spots from diffusing excessively and losing their proper shape and precision. The height of the spotting nozzle can be adjusted according to the specific characteristics of the sample to optimize its distribution on the thin-layer plate and improve the adaptability of the experiment. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0020] Figure 2 This utility model Figure 1 A magnified structural diagram of region A in the middle.

[0021] Figure 3 This is a rear-view three-dimensional structural diagram of the present invention.

[0022] Figure 4 This is a schematic diagram of the structure of the electric push rod 1, electric push rod 2, and support frame of this utility model.

[0023] Figure 5 This utility model Figure 4 A magnified structural diagram of region B in the middle.

[0024] The above figures include the following reference numerals:

[0025] 1. Heated sample dispenser; 2. Support base; 3. Auxiliary rod; 4. Motor; 5. Threaded rod; 6. Sample dispensing arm; 7. Air pump; 8. Sliding plate; 9. Electric push rod one; 10. Support frame; 11. Sample dispensing nozzle; 12. Air tube; 13. Extension plate; 14. Electric push rod two; 15. Threaded column; 16. Squeezing pad; 17. Support spring; 18. Sliding column; 19. Bending rod; 20. Clamping sleeve; 21. Air-water separator; 22. Limiting telescopic rod. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Embodiments of this utility model:

[0028] like Figures 1 to 5 As shown, an electric thin-layer chromatography spotter includes a heated spotter 1. A support base 2 is fixedly connected to the upper surface of the heated spotter 1. An auxiliary rod 3 is fixedly connected to the inner wall of the support base 2. A small motor 4 is fixedly connected to the outer wall of the support base 2. A threaded rod 5 is rotatably connected to the inner wall of the support base 2. The output shaft of the small motor 4 passes through one end of the support base 2 and is fixedly connected to the threaded rod 5. A spotting arm 6 is threadedly connected to the outer surface of the threaded rod 5. The spotting arm 6 is hollow inside and has a vertical groove on its outer surface. A sliding plate 8 is slidably connected inside the spotting arm 6. The inner wall of the spotting arm 6 is frosted to increase the friction with the sliding plate 8. The sliding plate 8 extends out of the groove, and the part extending out of the groove is U-shaped. An electric push rod 9 is fixedly connected to the outer wall of one end of the sliding plate 8 extending out of the groove, and a limit telescopic rod 22 is fixedly connected to the outer wall of the other end of the sliding plate 8 extending out of the groove. A support frame 10 is fixedly connected to the telescopic end of the electric push rod 9. A hole is opened on the support frame 10 for the sampling needle to pass through. A sampling nozzle 11 is fixedly connected to the lower part of the support frame 10. An air pump 7 and an air-water separator 21 are installed on one side of the heating sampling device 1. The air outlet of the air pump 7 is connected to the air-water separator 21 through an air pipe, and the air outlet of the air-water separator 21 is connected to the sampling nozzle 11 through an air pipe 12.

[0029] An extension plate 13 is fixedly connected to the upper surface of the support frame 10. An electric push rod 14 is fixedly connected to the extension plate 13. A pressure plate is fixedly connected to the telescopic end of the electric push rod 14.

[0030] The lower end of the sampling arm 6 is slidably connected to the auxiliary rod 3 to limit the sampling arm 6 to move only horizontally. The telescopic end of the limiting telescopic rod 22 is fixedly connected to the outer surface of the support frame 10 to limit the support frame 10. It can only move horizontally in the telescopic direction of the electric push rod 9 to ensure the balance of the horizontal movement of the support frame 10.

[0031] Two protrusions are fixedly connected to the upper surface of the support frame 10. Sliding posts 18 are slidably connected to each of the two protrusions. Support springs 17 are sleeved on the outer surfaces of the two sliding posts 18. Bending rods 19 are fixedly connected to one end of each sliding post 18 that passes through the inner sidewall of the protrusion. Clamping sleeves 20 are fixedly connected to the two ends of the two bending rods 19 that are close to each other. The two clamping sleeves 20 are set in an arc shape. When the two clamping sleeves 20 are close to each other, they form a circle. One end of the support spring 17 is fixed to the end of the sliding post 18 that is away from the support frame 10. The other end of the support spring 17 is fixedly connected to the protrusion on the upper surface of the support frame 10.

[0032] A threaded post 15 is threadedly connected to one side wall of the sliding plate 8 near the electric push rod 9. A compression pad 16 is fixedly connected to one end of the threaded post 15 that passes through the sliding plate 8. The compression pad 16 is made of rubber.

[0033] It should be noted that: the heating spotter 1 is equipped with a heater inside, the upper surface of the heating spotter 1 is a heating platform, and the heating spotter 1 is also equipped with a controller. The controller is electrically connected to the small motor 4, electric push rod 9, electric push rod 14, gas-water separator 21, and air pump 7 respectively. The heating spotter 1 is existing technology and will not be described in detail here.

[0034] The working process of the above embodiments:

[0035] Upon first use, manually press the two sliding posts 18 on both sides above the support frame 10, causing the two sliding posts 18 to move the bent rod 19. (Refer to...) Figure 5 Since the two bent rods 19 are connected to the clamping sleeves 20 respectively, when the two bent rods 19 move, they will cause the two clamping sleeves 20 to move away from each other. During this process, the sliding column 18 will compress the corresponding support spring 17. After the two clamping sleeves 20 move away from each other, the sampling needle for absorbing the sample solution is placed between the two clamping sleeves 20 from top to bottom, so that the tip of the sampling needle is inserted into the support frame 10. Then, the two pressed sliding columns 18 are released. At this time, under the reset and rebound action of the support spring 17, the two bent rods 19 will cause the two clamping sleeves 20 to move closer to each other and clamp the sampling needle firmly, so as to avoid the sampling needle from shaking, shifting or even falling off during the sampling process, and ensure the stability of the sampling process.

[0036] It should be added that the sampling needle works on the same principle as a syringe. There is a plunger inside the sampling needle. There is a good seal between the plunger and the inner wall of the syringe. When the plunger is pressed, it applies pressure to the liquid in the syringe, causing the liquid to be squeezed and then atomized into a spray from the needle tip.

[0037] Next, the thin-layer plate is placed on the upper surface of the heated sample applicator 1. The heater of the heated sample applicator 1 is turned on by the controller to heat the thin-layer plate. Then, the small motor 4, the electric push rod 14, the air pump 7, and the air-water separator 21 are started by the controller. The output shaft of the small motor 4 drives the threaded rod 5 to rotate. The threaded rod 5 drives the sample arm 6, which is threaded to it, to move horizontally on the auxiliary rod 3. During the process, the electric push rod 14 is activated, and its telescopic end descends, causing the pressure plate to press the push rod on the sample needle, so that the sample solution in the sample needle is discharged into the support frame 10 and enters the sample nozzle 11. At this time, the air pump 7 compresses the gas, and then discharges the compressed gas into the gas-water separator 21 through the pipeline. It is then stably delivered to the spotting nozzle 11 through the air pipe 12, so that the sample solution can be sprayed from the spotting nozzle 11 onto the thin-layer plate. With the help of the threaded rod 5, the spotting arm 6 and the spotting nozzle 11 move horizontally, so that the sample can be evenly spread on the thin-layer plate to form a strip with a relatively high concentration and a more regular and uniform sample distribution. When combined with subsequent quantitative analysis methods such as optical density scanning, the content of each component can be more accurately determined, which helps to improve the accuracy of quantitative analysis.

[0038] When the gas compressed by the air pump 7 is filtered through the gas-water separator 21, water vapor condenses into small water droplets on the surface of the gas-water separator 21, while the gas continues to pass smoothly, achieving water vapor separation. Only the dry gas flows to the sampling nozzle 11. If the gas contains a lot of water vapor, the water vapor may mix into the sample, causing the sample to be diluted. Water vapor separation ensures the stability of the sample concentration, making the sampling amount more accurate and controllable, which is conducive to precise analysis. The dry gas after water vapor separation allows the sample to be more regularly distributed on the thin-layer plate, which helps to improve the separation resolution, better observe and analyze the band characteristics corresponding to each component, and avoid water vapor mixing, which would change the physical properties of the sample and cause the bands formed on the thin-layer plate to diffuse or have irregular shapes.

[0039] Furthermore, after the threaded rod 5 rotates forward to drive the spotting arm 6 to move horizontally for spotting and form a spotting strip, the controller can activate the electric push rod 9, causing its telescopic end to push the support frame 10 and the mechanism on it to move horizontally away from the spotting arm 6. During this process, the limit telescopic rod 22 will extend accordingly. Then, the controller can start the output shaft of the small motor 4 to drive the threaded rod 5 to reverse, which will drive the moving spotting arm 6 to move horizontally and reset again. During the resetting process, the electric push rod 14, the air pump 7, and the air-water separator 21 are activated again, so that the sample is atomized and sprayed evenly on the thin-layer plate to form a strip. If precise quantitative analysis of the components in a sample is required, multiple spottings can be repeated to form multiple parallel bands. The average value of the detection results corresponding to these bands can then be taken to reduce errors and improve detection accuracy. Furthermore, the heating temperature of the thin-layer plate can be adjusted by the controller to analyze the performance of the same sample components at different temperatures. That is, a band can be formed by spotting the sample on the same thin-layer plate first, then the position can be moved, the heating temperature of the thin-layer plate can be adjusted, and a new band can be formed by spotting the sample again. Then, each band can be developed and analyzed under different temperature conditions without the need to manually move the thin-layer plate, thus achieving multiple spottings.

[0040] It should be noted that electric linear actuator 1.9 and electric linear actuator 2.14 can be LAM miniature electric linear actuators.

[0041] It can drive the support frame 10, the sampling needle and the pressure plate to move and stop multiple times in order to perform multiple rows of sampling.

[0042] Furthermore, in actual operation, the threaded column 15 can be rotated by hand to gradually move the squeezing pad 16 away from the outer wall of the spotting arm 6. Then, the sliding plate 8 can be moved up and down on the spotting arm 6 to adjust the distance between the spotting nozzle 11 and the thin-layer plate. After adjustment, the threaded column 15 is rotated again to make the squeezing pad 16 fit tightly against the outer surface of the spotting arm 6, thereby limiting the height of the spotting nozzle 11. Different samples have different physical properties such as viscosity and density. At the same distance between the nozzle and the thin-layer plate, their distribution on the thin-layer plate will be different. For example, for samples with higher viscosity, the distance needs to be increased appropriately to allow the atomized droplets to disperse more fully and avoid sticking together. For samples that are thinner and easier to diffuse, the distance needs to be reduced to prevent the sample spots from diffusing excessively and losing their proper shape and precision. Adjusting the distance can optimize the distribution on the thin-layer plate according to the specific characteristics of the sample, thereby improving the adaptability of the experiment.

[0043] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0044] The above embodiments are only used to illustrate the technical solutions of this utility model and are not intended to limit the scope of protection of this utility model. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the essence and scope of the technical solutions of this utility model.

Claims

1. An electric thin-layer chromatography spotter, comprising a heated spotter (1), characterized in that: A support base (2) is fixedly connected to the upper surface of the heating sample applicator (1). An auxiliary rod (3) is fixedly connected to the inner wall of the support base (2). A motor (4) is fixedly connected to the outer wall of the support base (2). A threaded rod (5) is rotatably connected to the inner wall of the support base (2). The output shaft of the motor (4) passes through one end of the support base (2) and is fixedly connected to the threaded rod (5). A sample applicator arm (6) is threadedly connected to the outer surface of the threaded rod (5). A sliding plate (8) is slidably connected to the sample applicator arm (6). One end of the sliding plate (8) is fixedly connected to... An electric push rod (9) is connected to the other end of the sliding plate (8), and a limit telescopic rod (22) is fixedly connected to the telescopic end of the electric push rod (9). A support frame (10) is fixedly connected to the telescopic end of the support frame (10). A sampling nozzle (11) is fixedly connected to the lower part of the support frame (10). An air pump (7) and an air-water separator (21) are respectively installed on one side of the heating sampling device (1). The air outlet of the air pump (7) is connected to the air-water separator (21). The air outlet of the air-water separator (21) is connected to the sampling nozzle (11) through an air pipe (12).

2. The electric thin-layer chromatography spotter according to claim 1, characterized in that: An extension plate (13) is fixedly connected to the upper surface of the support frame (10), and an electric push rod (14) is fixedly connected to the extension plate (13).

3. The electric thin-layer chromatography spotter according to claim 1, characterized in that: The end of the sampling arm (6) near the bottom is slidably connected to the auxiliary rod (3), and the telescopic end of the limiting telescopic rod (22) is fixedly connected to the outer surface of the support frame (10).

4. The electric thin-layer chromatography spotter according to claim 1, characterized in that: Two protrusions are fixedly connected to the upper surface of the support frame (10). Sliding columns (18) are slidably connected to both protrusions. Support springs (17) are sleeved on the outer surfaces of both sliding columns (18). Bending rods (19) are fixedly connected to one end of each sliding column (18) that passes through the inner sidewall of the protrusion. Clamping sleeves (20) are fixedly connected to the two bending rods (19) that are close to each other.

5. The electric thin-layer chromatography spotter according to claim 4, characterized in that: The two clamping sleeves (20) are set in an arc shape. One end of the support spring (17) is fixed to the end of the sliding column (18) away from the support frame (10), and the other end of the support spring (17) is fixedly connected to the protrusion on the upper surface of the support frame (10).

6. The electric thin-layer chromatography spotter according to claim 1, characterized in that: A threaded post (15) is threaded to one side wall of the sliding plate (8) near the electric push rod (9). A compression pad (16) is fixedly connected to one end of the threaded post (15) that passes through the sliding plate (8). The compression pad (16) is made of rubber. The inner wall of the sampling arm (6) is sanded.

7. The electric thin-layer chromatography spotter according to claim 1, characterized in that: The sampling arm (6) is hollow inside, and the outer surface is provided with a groove for sliding the sliding plate (8). One end of the sliding plate (8) is located in the groove, and the other end extends out of the groove. The part extending out of the groove is U-shaped.

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