Efficient cell separation device

Abstract

The utility model relates to the technical field of cell separation, in particular to an efficient cell separation device which comprises a separation tank, a bottom plate, a rotating shaft, a scraping plate, a blade, a through hole and a fixing plate, a fixing seat is fixedly connected to the top of the bottom plate, an adjusting opening is formed in the side wall of the fixing seat, and the top of the fixing seat is rotationally connected with the bottom of the separation tank; the side wall of the separation tank is provided with a discharge port and a water pump, the top of the bottom plate is fixedly connected with a motor, the output end of the motor is fixedly connected with a rectangular block, the top of the rectangular block is fixedly connected with the bottom of the rotating shaft, the outer side wall of the rectangular block is slidably connected with a gear, and the bottom of the separation tank is fixedly connected with an inner gear ring; according to the adipose tissue cutting device, the position of the gear can be conveniently limited through the arranged limiting assembly, and the technical purpose that the adipose tissue cutting device cuts adipose tissue or rapidly separates cells is achieved through clamping and separating work between the gear and the inner gear ring.

Description

Technical Field

[0001] This utility model relates to the field of cell separation technology, specifically to a high-efficiency cell separation device. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated daughter cell. The definition of stem cells has been revised over the years and defined from different perspectives. Currently, most biologists and medical professionals believe that stem cells are a type of cell derived from embryos, fetuses, or adults, possessing the ability to self-renew, proliferate, and differentiate without limitation under certain conditions. They can produce daughter cells with identical phenotypes and genotypes, as well as specialized cells that make up body tissues and organs, and can also differentiate into progenitor cells.

[0003] For example, patent CN214422652U discloses a highly efficient stem cell separation device, which solves the problems of time-consuming manual shaking and vibration, low work efficiency, and uneven grinding of adipose tissue in existing technologies when the workload is large, which affects stem cell separation. The highly efficient stem cell separation device includes a base plate, a separation box located at the center of the top of the base plate, a first drive motor fixedly connected to the center of the top of the separation box by bolts, a partition in the inner cavity of the separation box, and the output shaft of the first drive motor passing through the top of the separation box via a bearing sleeve. A crushing rod is driven to the extended end of the output shaft of the first drive motor, and several blades are rotatably connected to the outer wall of the crushing rod via a rotating shaft.

[0004] With the above-described design, the existing technology can handle large-scale separation tasks, eliminating the need for manual hand-cranking and vibration, facilitating more uniform shearing of adipose tissue, saving manpower, and improving separation efficiency. However, the existing high-efficiency stem cell separation device has the following drawbacks during operation:

[0005] When using the above-mentioned device, the adipose tissue is first placed inside the separation box, then the adipose tissue is cut by a blade, and then the separated adipose tissue is shaken back and forth to separate the cells. However, the cell separation component is slow when shaking, and it is easy to cause the cells to fuse with the plasma, thus making it impossible to separate the cells. Utility Model Content

[0006] The present invention aims to provide a highly efficient cell separation device, which is mainly used to solve the technical problem that the cell separation efficiency is relatively slow in the prior art.

[0007] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0008] A high-efficiency cell separation device includes a separation tank, a base plate, a rotating shaft, a scraper, a blade, a through hole, and a fixing plate. A fixing seat is fixedly connected to the top of the base plate, and an adjustment port is provided on the side wall of the fixing seat. The top of the fixing seat is rotatably connected to the bottom of the separation tank. A discharge port is provided on the side wall of the separation tank, and a water pump is provided on the side wall of the separation tank. A motor is fixedly connected to the top of the base plate, and a rectangular block is fixedly connected to the output end of the motor. The top of the rectangular block is fixedly connected to the bottom of the rotating shaft, and a gear is slidably connected to the outer side wall of the rectangular block. An internal gear ring is fixedly connected to the bottom of the separation tank, and the internal gear ring is adapted to the gear. An installation groove is provided on the side wall of the rectangular block, and a limit component is slidably connected to the inner side wall of the installation groove.

[0009] The working principle and beneficial effects of this utility model:

[0010] 1. Working Principle: When using this device, the adipose tissue is first placed inside the separation tank. Then, by starting the motor, the rectangular block drives the rotating shaft to rotate. During the rotation of the shaft, the adipose tissue is cut by the blades. The cut adipose tissue falls through the through hole to the bottom of the separation tank. Then, the gear is pushed upward, causing the gear to slide upward along the outer wall of the rectangular block, thereby engaging with the internal gear ring. The gear is then limited by the limiting component. At this time, during the rotation of the shaft, the gear drives the internal gear ring to rotate, which in turn drives the separation tank to rotate. During the rotation of the separation tank, the cells inside the adipose tissue are separated. Finally, the separated cells or plasma are discharged through the outlet or water pump.

[0011] 2. Beneficial effects:

[0012] The existing technology can handle large-scale separation work without manual hand-cranking, making it easier to cut adipose tissue more evenly, saving manpower and improving separation efficiency. However, the existing technology has the problem of slow cell separation efficiency. This solution solves the problem of the device cutting adipose tissue or separating cells by setting a limiting component to limit the position of the gear.

[0013] Preferably, the limiting component includes a movable plate slidably connected to the inner wall of the mounting groove. An abutment block is fixedly connected to the outer wall of the movable plate, with the top of the abutment block abutting against the bottom of the gear. An adjusting rod is fixedly connected to the outer wall of the movable plate. In use, the adipose tissue is first placed into the separation tank. Then, by starting the motor, the rectangular block drives the rotating shaft to rotate. During the rotation, the blade cuts the adipose tissue, which falls through the through-hole to the bottom of the separation tank. When it is necessary to separate the cells inside the adipose tissue, the adjusting rod is pressed to move the movable plate along the inner wall of the mounting groove, causing the abutment block to move into the mounting groove. Then, the gear is pushed upwards, moving it upwards along the outer wall of the rectangular block until it engages with the internal gear ring. The top of the abutment block abuts against the bottom of the gear, limiting the gear's position. During the rotation of the shaft, the gear and internal gear ring work together to rotate the separation tank, thus achieving cell separation.

[0014] Preferably, the limiting component further includes a spring fixedly connected to the inner wall of the mounting groove, and the other end of the spring is fixedly connected to the inner wall of the moving plate. During the movement of the moving plate along the inner wall of the mounting groove, the spring is compressed. When the gear engages with the internal gear ring, the force applied to the adjusting rod is released, and the moving plate is reset under the action of the compressed spring, thereby achieving the technical problem of the contact block automatically limiting the gear.

[0015] Preferably, the water pump is fixedly connected to a telescopic pipe at one end inside the separation tank, and a buoyancy plate is provided at the bottom of the telescopic pipe. The water pump is provided with a water outlet at the other end outside the separation tank. After the separation tank is rotated, the cells will settle at the bottom of the separation tank, and the upper layer of the separated cells will be plasma. The buoyancy plate will float on the top of the plasma, so the plasma can be drawn out by the water pump. Finally, the separated cells can be discharged through the water outlet.

[0016] Preferably, a sealing ring is provided at the connection between the separator and the rotating shaft to prevent liquid from flowing out from there.

[0017] Preferably, the range of motion of the gear is much greater than the thickness of the internal gear ring, which prevents the gear from disengaging from the internal gear ring even when it reaches the point of maximum contact. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0019] Figure 2 This is a schematic diagram of the internal structure of the separator of this utility model;

[0020] Figure 3This is a schematic diagram of the rectangular block, gear, and internal gear ring of this utility model;

[0021] Figure 4 This is a schematic diagram of the internal structure of the mounting slot of this utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the buoyancy plate, telescopic pipe and water pump of this utility model.

[0023] The reference numerals in the accompanying drawings of the instruction manual include: 1. Separator; 2. Fixing base; 3. Water pump; 4. Discharge port; 5. Base plate; 6. Rotating shaft; 7. Scraper; 8. Blade; 9. Through hole; 10. Sealing ring; 11. Fixing plate; 12. Gear; 13. Motor; 14. Internal gear ring; 15. Telescopic tube; 16. Mounting groove; 17. Rectangular block; 18. Spring; 19. Moving plate; 20. Abutment block; 21. Adjusting rod; 22. Buoyancy plate; 23. Water outlet. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] like Figure 1-5As shown, a high-efficiency cell separation device includes a separation tank 1, a base plate 5, a rotating shaft 6, a scraper 7, a blade 8, a through hole 9, and a fixing plate 11. A fixing seat 2 is fixedly connected to the top of the base plate 5. An adjustment port is provided on the side wall of the fixing seat 2. The top of the fixing seat 2 is rotatably connected to the bottom of the separation tank 1. A discharge port 4 is provided on the side wall of the separation tank 1. A water pump 3 is installed on the side wall of the separation tank 1. A telescopic pipe 15 is fixedly connected to one end of the water pump 3 inside the separation tank 1. A buoyancy plate 22 is provided at the bottom of the telescopic pipe 15. A water outlet 23 is provided at the other end of the water pump 3 outside the separation tank 1. After the separation tank 1 rotates, the cells will settle at the bottom of the separation tank 1. The upper layer of the separated cells is plasma, and the buoyancy plate 22... 2 will float on top of the plasma, and the plasma will be drawn out by the water pump 3. Finally, the separated cells will be discharged through the discharge port 4. The top of the bottom plate 5 is fixedly connected to the motor 13, and the output end of the motor 13 is fixedly connected to the rectangular block 17. The top of the rectangular block 17 is fixedly connected to the bottom of the rotating shaft 6. The outer side wall of the rectangular block 17 is slidably connected to the gear 12. The bottom of the separation tank 1 is fixedly connected to the internal gear ring 14, which is adapted to the gear 12. The side wall of the rectangular block 17 is provided with an installation groove 16. The inner side wall of the installation groove 16 is slidably connected to a limit component. The limit component includes a moving plate 19 slidably connected to the inner side wall of the installation groove 16. The outer side wall of the moving plate 19 is fixedly connected to an abutment. Block 20, the top of which abuts against the bottom of gear 12, and an adjusting rod 21 is fixedly connected to the outer wall of the moving plate 19. The limiting assembly also includes a spring 18 fixedly connected to the inner wall of the mounting groove 16, and the other end of the spring 18 is fixedly connected to the inner wall of the moving plate 19. When the device is in use, the adipose tissue is first placed into the separation tank 1, and then the motor 13 is started, and the rectangular block 17 drives the rotating shaft 6 to rotate. During the rotation of the rotating shaft 6, the blade 8 cuts the adipose tissue. The cut adipose tissue falls to the bottom of the separation tank 1 through the through hole 9. When it is necessary to separate the cells inside the adipose tissue, the adjusting rod 21 is pressed first to make the moving plate 19 move along the... The contact block 20 moves along the inner wall of the mounting groove 16, thereby moving the contact block 20 into the mounting groove 16. During this process, the spring 18 is compressed, and then the gear 12 is pushed upward, causing the gear 12 to move upward along the outer wall of the rectangular block 17 until the gear 12 and the inner gear ring 14 are engaged. After the gear 12 and the inner gear ring 14 are engaged, the force applied to the adjusting rod 21 is released, and the moving plate 19 is reset under the action of the compressed spring 18. This achieves the technical problem of the contact block 20 automatically limiting the gear 12. At this time, during the rotation of the rotating shaft 6, the separation tank 1 can be driven to rotate through the mutual cooperation between the gear 12 and the inner gear ring 14, thereby realizing the separation of cells.

[0026] As can be seen from the above, the specific embodiments of this utility model are as follows:

[0027] In use, the adipose tissue is first placed inside the separation tank 1. Then, by starting the motor 13, the rectangular block 17 drives the rotating shaft 6 to rotate. During the rotation of the shaft 6, the blade 8 cuts the adipose tissue. The cut adipose tissue falls to the bottom of the separation tank 1 through the through hole 9. When it is necessary to separate the cells inside the adipose tissue, the adjusting rod 21 is pressed to move the moving plate 19 along the inner wall of the mounting groove 16, thereby moving the contact block 20 into the mounting groove 16. During this process, the spring 18 is compressed, and then the gear 12 is pushed upward, causing the gear 12 to move upward along the outer wall of the rectangular block 17 until the gear 12 engages with the inner gear ring 1. 4. When gear 12 engages with internal gear ring 14, the force applied to adjusting rod 21 is released, and moving plate 19 is reset under the action of compression spring 18. This achieves the technical problem of automatic limiting of gear 12 by contact block 20. At this time, during the rotation of rotating shaft 6, the interaction between gear 12 and internal gear ring 14 can drive separation tank 1 to rotate, thereby realizing the separation of cells. After separation tank 1 rotates, cells will settle at the bottom of separation tank 1, and the upper layer of separated cells will be plasma. Buoyancy plate 22 will float on top of plasma, and plasma will be drawn out by water pump 3. Finally, the separated cells can be discharged through discharge port 4.

[0028] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A high-efficiency cell separation device, comprising a separation tank (1), a bottom plate (5), a rotating shaft (6), a scraper (7), a blade (8), a through hole (9), and a fixing plate (11), characterized in that, A fixed seat (2) is fixedly connected to the top of the base plate (5). An adjustment port is provided on the side wall of the fixed seat (2). The top of the fixed seat (2) is rotatably connected to the bottom of the separation tank (1). A discharge port (4) is provided on the side wall of the separation tank (1). A water pump (3) is provided on the side wall of the separation tank (1). A motor (13) is fixedly connected to the top of the base plate (5). A rectangular block (17) is fixedly connected to the output end of the motor (13). The top of the rectangular block (17) is fixedly connected to the bottom of the rotating shaft (6). A gear (12) is slidably connected to the outer side wall of the rectangular block (17). An internal gear ring (14) is fixedly connected to the bottom of the separation tank (1). The internal gear ring (14) is adapted to the gear (12). An installation groove (16) is provided on the side wall of the rectangular block (17). A limit component is slidably connected to the inner side wall of the installation groove (16).

2. The high-efficiency cell separation device according to claim 1, characterized in that: The limiting component includes a movable plate (19) slidably connected to the inner side wall of the mounting groove (16), an abutment block (20) fixedly connected to the outer side wall of the movable plate (19), the top of the abutment block (20) abutting against the bottom of the gear (12), and an adjusting rod (21) fixedly connected to the outer side wall of the movable plate (19).

3. The high-efficiency cell separation device according to claim 2, characterized in that: The limiting component also includes a spring (18) fixedly connected to the inner wall of the mounting groove (16), and the other end of the spring (18) is fixedly connected to the inner wall of the movable plate (19).

4. The high-efficiency cell separation device according to claim 1, characterized in that: The water pump (3) is fixedly connected to a telescopic pipe (15) at one end inside the separation tank (1). A buoyancy plate (22) is provided at the bottom of the telescopic pipe (15). The water pump (3) is provided with an outlet (23) at one end outside the separation tank (1).

5. The high-efficiency cell separation device according to claim 1, characterized in that: A sealing ring (10) is provided at the connection between the separation tank (1) and the rotating shaft (6).

6. The high-efficiency cell separation device according to claim 1, characterized in that: The range of motion of the gear (12) is much greater than the thickness of the internal gear ring (14).

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