Modular cell culture pipeline system

Abstract

The utility model discloses a modularized cell culture pipeline system and relates to the technical field of cell culture. The device comprises a flow guide box, the top of the flow guide box is communicated with a plurality of culture medium conveying assemblies through first pipelines, the bottom of the flow guide box is communicated with a plurality of material mixing assemblies, a partition plate penetrates through and is movably connected to the interior of the flow guide box, and the interior of the flow guide box is divided into a first cavity and a second cavity by the partition plate; the culture container is respectively communicated with the plurality of groups of material mixing assemblies through a plurality of second pipelines, and regulating valves are communicated between the second pipelines and the material mixing assemblies. Through the arrangement of the flow guide box and the multiple groups of material mixing assemblies, the mixing uniformity of various culture media is improved, the cell culture quality and efficiency are ensured, the multiple groups of culture medium conveying assemblies support simultaneous conveying of the culture media with different components, and the complex culture requirements are met.

Description

Technical Field

[0001] This utility model belongs to the field of cell culture, and more specifically, relates to a modular cell culture pipeline system. Background Technology

[0002] Cell culture technology is one of the core technologies in biomedicine, biopharmaceuticals and other fields. It is widely used in stem cell research, vaccine production and gene therapy. With the rapid development of cell therapy and personalized medicine, higher requirements are placed on the flexibility, controllability and scalability of cell culture systems. Among them, the cell culture tubing system is a system used to transport and distribute culture medium (culture medium, gas, etc.) during the cell culture process. It is composed of multiple modules with specific functions connected by tubing. However, the existing cell culture tubing is relatively messy and the connection with the cell culture tank is also relatively complicated, making it difficult to install and maintain, and requiring a lot of manpower and time.

[0003] Chinese patent CN214496266U discloses a tubing arrangement structure for cell culture jars. This device, through the sequential arrangement of tubing, does not occupy much space, ensures safe use, and is simple and uncluttered. During installation, the mounting ring is first rotated, causing the torsion spring inside the channel to compress and align the slider on the inner wall with the groove. The mounting ring is then installed on the mounting block until the slider enters the annular groove from the groove. Releasing the mounting ring causes the torsion spring to rotate, displacing the slider from one end of the groove. The annular groove then locks the slider in place, securing the tubing. However, this device can only deliver a single culture medium to the culture jar for mixing within the jar. This can lead to uneven mixing of some culture media that require mixing to achieve their full effect, affecting cell culture.

[0004] In view of this, this utility model is proposed. Utility Model Content

[0005] The technical problem to be solved by this invention is to overcome the shortcomings of the prior art and provide a modular cell culture pipeline system, which solves the problems mentioned in the background art.

[0006] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is as follows:

[0007] A modular cell culture piping system includes: a flow box, the top of which is connected to multiple sets of culture medium delivery components via a first pipe, the bottom of which is connected to multiple sets of mixing components, a partition that runs through and is movably connected to the inside of the flow box, the partition dividing the inside of the flow box into a first chamber and a second chamber, and an adjustment component for adjusting the partition on the flow box.

[0008] The culture container is connected to multiple mixing components via multiple second pipes, and a regulating valve is connected between the second pipes and the mixing components.

[0009] Optionally, the mixing assembly includes a mixing pipe and a mixing plate. The mixing pipe is connected to the flow guide box and the regulating valve. The mixing plate is fixedly installed inside the mixing pipe. The mixing plate has a spiral structure and forms a spiral channel inside the mixing pipe.

[0010] Optionally, a flow-guiding funnel is connected between the mixing pipe and the flow guide box.

[0011] Optionally, the adjustment component includes:

[0012] A mounting bracket is fixedly mounted on the air guide box, and a threaded rod is movably provided through the mounting bracket, the threaded rod being rotatably disposed on the top of the partition plate;

[0013] A worm gear is sleeved and threadedly connected to the threaded rod. The worm gear is rotatably mounted on the mounting bracket, and a worm that meshes with the worm gear is rotatably connected to the mounting bracket.

[0014] Optionally, the two ends of the partition are fixedly connected to a bonding plate, and a sealing gasket is fixedly installed on the bonding plate.

[0015] Optionally, a third pipe is connected between the first pipe and the flow guide box, and the third pipe is detachably connected to the first pipe via a Luer joint.

[0016] Optionally, a one-way valve is connected between the third pipe and the flow guide box.

[0017] Optionally, the flow guide box is detachably connected with a box cover.

[0018] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art. Of course, any product implementing the present invention does not necessarily need to achieve all of the following advantages at the same time:

[0019] 1. By setting up mixing components, flow guide boxes, adjustment components, and baffles, the flow guide boxes and multiple sets of mixing components improve the mixing uniformity of various culture media, ensuring the quality and efficiency of cell culture. Multiple sets of culture media delivery components support the simultaneous delivery of culture media with different components to meet complex culture needs. At the same time, the modular design between multiple structures facilitates independent replacement or upgrades.

[0020] 2. Equipped with Luer connectors and check valves, the standardized design of the Luer connectors supports rapid connection of pipes and components of different specifications, and the connectors have good sealing performance, reducing the risk of leakage and microbial intrusion; the check valves ensure that the culture medium can only flow into the diversion box from the third pipe, preventing backflow.

[0021] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0022] The accompanying drawings described below are merely some embodiments. Those skilled in the art can obtain other drawings based on these drawings without any creative effort. In the drawings:

[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0024] Figure 2 This is a front view of the present invention;

[0025] Figure 3 This is a schematic diagram of the structure of the flow guide box of this utility model;

[0026] Figure 4 This is a schematic diagram of the structure of the partition of this utility model;

[0027] Figure 5 This utility model Figure 4 Enlarged structural diagram at point A;

[0028] Figure 6 This is a schematic diagram of the mixing component of this utility model.

[0029] The attached diagram lists the components represented by each number as follows:

[0030] 1. Flow guide box; 2. Culture container; 3. Culture medium delivery assembly; 31. Pump body; 32. Delivery pipeline; 33. Storage tank; 4. Check valve; 5. Luer connector; 6. First pipeline; 7. Regulating valve; 8. Mixing assembly; 81. Mixing pipe; 82. Mixing plate; 9. Regulating assembly; 91. Threaded rod; 92. Mounting bracket; 93. Worm gear; 94. Worm; 10. Second pipeline; 11. Third pipeline; 12. Drainage funnel; 13. Box cover; 14. First chamber; 15. Second chamber; 16. Partition; 17. Adhesive plate; 18. Sealing gasket.

[0031] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0032] The present invention will now be described in further detail with reference to the accompanying drawings.

[0033] Please see Figure 1-6 As shown, this embodiment provides a modular cell culture piping system, including a flow guide box 1, the top of which is connected to multiple sets of culture medium delivery components 3 through a first pipe 6, the bottom of which is connected to multiple sets of mixing components 8, a partition 16 running through and movably connected inside the flow guide box 1, the partition 16 dividing the inside of the flow guide box 1 into a first chamber 14 and a second chamber 15, the flow guide box 1 is provided with an adjustment component 9 for adjusting the partition 16, and a culture container 2, which is connected to multiple sets of mixing components 8 through multiple second pipes 10, and an adjustment valve 7 connecting the second pipes 10 and the mixing components 8.

[0034] Specifically, in this embodiment, the culture medium delivery assembly 3 includes a pump body 31 (peristaltic pump), a storage tank 33, and a delivery pipe 32. The storage tank 33 is connected to the inlet of the pump body 31 through the delivery pipe 32, and the outlet of the pump body 31 is connected to the first pipe 6. The pump body 31 is controlled by an external control device. In this embodiment, there are four sets of culture medium delivery assemblies 3, with four first pipes 6 connected and arranged at the top of the guide box 1. There are three sets of mixing assemblies 8, arranged opposite each other between adjacent culture medium delivery assemblies 3. A partition 16 is positioned relative to the centrally located mixing assembly 8, and the partition 16 can seal the inlet of the mixing assembly 8. When it is necessary to mix the culture medium from multiple sets of culture medium delivery assemblies 3 in the first chamber 14 or the second chamber 15 and deliver it to the culture container 2, the partition 16 is located inside the guide box 1, serving a separating function. The culture medium is delivered to the first chamber 14 or the second chamber 15 by activating the corresponding pump body 31. The regulating valve 7 allows the culture medium in the first chamber 14 and the second chamber 15 to enter the mixing assembly 8 for mixing, and then discharges it into the culture container 2 through the second pipe 10. When it is necessary to mix the culture medium in the first chamber 14 and the second chamber 15, the regulating valve 7 corresponding to the first chamber 14 and the second chamber 15 is closed. The regulating assembly 9 drives the partition 16 to move to the outside of the guide box 1, so that the first chamber 14 and the second chamber 15 are connected. At the same time, the regulating valve 7 on the second pipe 10 corresponding to the partition 16 is opened. The multiple culture medium delivery assemblies 3 deliver the culture medium to the guide box 1, mix it through the mixing assembly 8, and finally discharge it into the culture container 2 through the second pipe 10. The overall structure is simple to operate, has multiple mixing mechanisms, improves the mixing uniformity of various culture media, and ensures the quality and efficiency of cell culture. The multiple culture medium delivery assemblies 3 support the simultaneous delivery of culture media with different components to meet complex culture needs. At the same time, the modular setting between the multiple structures makes it easy to replace or upgrade independently.

[0035] It should be noted that the culture medium delivery component 3, the mixing component 8 and the first pipe 6 are set in equal quantities. The number of culture medium delivery component 3 and mixing component 8 is not limited in other embodiments and can be adjusted according to the actual situation. Secondly, the bottom of the partition 16 can be set to fit against the inner wall of the flow guide box 1, and the flow guide box 1 is provided with a through groove for the partition 16 to move.

[0036] In this embodiment, as Figure 3 , Figure 4 and Figure 6 As shown, the mixing assembly 8 includes a mixing pipe 81 and a mixing plate 82. The mixing pipe 81 is connected to the flow guide box 1 and the regulating valve 7. The mixing plate 82 is fixedly installed inside the mixing pipe 81. The mixing plate 82 has a spiral structure and forms a spiral channel inside the mixing pipe 81. Specifically, the spiral mixing plate 82 inside the mixing pipe 81 forms a spiral channel, which allows the culture medium to rotate and mix along the spiral path during flow, enhancing the turbulence effect. The spiral channel extends the fluid path, promotes the full mixing of different culture medium components, and avoids local uneven concentration.

[0037] In this embodiment, as Figure 2 and Figure 4 As shown, a flow funnel 12 is connected between the mixing pipe 81 and the flow guide box 1. Specifically, the flow funnel 12 enlarges the connection diameter between the flow guide box 1 and the mixing pipe 81, guiding the culture medium to flow smoothly into the mixing pipe 81. It should be noted that in this embodiment, the regulating valve 7 can also be installed between the flow funnel 12 and the mixing pipe 81, which can prevent the culture medium from accumulating in the mixing pipe 81.

[0038] In this embodiment, as Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5As shown, the adjusting assembly 9 includes a mounting bracket 92, which is fixedly mounted on the guide box 1. A threaded rod 91 is movably mounted through the mounting bracket 92, and the threaded rod 91 is rotatably mounted on the top of the partition plate 16. A worm gear 93 is sleeved on and threadedly connected to the threaded rod 91, and the worm gear 93 is rotatably mounted on the mounting bracket 92. A worm 94 that meshes with the worm gear 93 is rotatably connected to the mounting bracket 92. Adhesive plates 17 are fixedly connected to both ends of the partition plate 16, and sealing gaskets 18 are fixedly mounted on the adhesive plates 17. Specifically, by rotating the worm gear 94, the worm gear 93 is driven to rotate, and the worm gear 93 drives the threaded rod 91 to move up and down. The position of the baffle 16 within the flow box 1 is adjusted by the reverse self-locking characteristic of the worm gear 93 and worm 94 transmission, ensuring that the position of the baffle 16 is locked and preventing displacement due to vibration during the culture process. At the same time, the sealing design ensures that the culture medium in different chambers flows independently, avoiding experimental errors caused by component mixing. It should be noted that two bonding plates 17 are fixedly installed on the baffle 16 vertically. The threaded rod 91 rotates on the top baffle 16. Meanwhile, the bonding plates 17 can block the inlet of the corresponding flow funnel. The sealing gasket 18 is fixedly installed on the end face of the bonding plates 17 and the baffle 16 that contacts the flow box 1.

[0039] In this embodiment, as Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, a third pipe 11 connects the first pipe 6 to the flow guide box 1. The third pipe 11 is detachably connected to the first pipe 6 via a Luer connector 5. Specifically, the standardized design of the Luer connector 5 supports the rapid docking of pipes and components of different specifications, and the joint has good sealing performance, reducing the risk of leakage and microbial intrusion. It should be noted that in this embodiment, the Luer connector 5 is a standardized micro-leakage-free connector, which is connected by a male Luer connector and a matching female Luer connector. It is now widely used in medical and laboratory instruments. At the same time, in this embodiment, the regulating valve 7 is a Luer interface regulating valve (made of polypropylene (PP) or polycarbonate (PC), and the flow rate is adjusted and the switch is controlled by rotating the handle). The second pipe 10 is connected to the regulating valve 7 via the Luer connector 5. The advantage of this is that it facilitates the rapid disassembly and assembly of each pipe, facilitates subsequent maintenance and replacement, realizes modular setting, and improves the service life and versatility of the device.

[0040] In this embodiment, as Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, a one-way valve 4 is connected between the third pipe 11 and the flow box 1. Specifically, the one-way valve 4 ensures that the culture medium can only flow into the flow box 1 from the third pipe 11 to prevent backflow.

[0041] In this embodiment, as Figure 3 and Figure 4 As shown, a cover 13 is detachably connected to the air guide box 1. Specifically, the cover 13 is detachably connected to the top of the air guide box 1 to facilitate internal cleaning and maintenance.

[0042] Working principle:

[0043] When it is necessary to mix the culture medium from multiple culture medium delivery components 3 in the first chamber 14 or the second chamber 15 and deliver it to the culture container 2, the partition 16 is located inside the guide box 1 and acts as a separator. The corresponding pump 31 is activated to deliver the culture medium into the first chamber 14 or the second chamber 15. By opening the regulating valve 7 corresponding to the first chamber 14 or the second chamber 15, the culture medium in the first chamber 14 and the second chamber 15 enters the mixing pipe 81, causing the culture medium to rotate and mix along a spiral path during flow, and then discharge it into the culture container 2 through the second pipe 10. When it is necessary to mix the culture medium in the first chamber 14 and the second chamber 15, the regulating valve 7 corresponding to the first chamber 14 and the second chamber 15 is closed, and the spiral is rotated... Rod 94 drives worm gear 93 to rotate, and worm gear 93 drives threaded rod 91 to move up and down, thereby adjusting the position of partition 16 in the flow box 1, so that the first chamber 14 and the second chamber 15 are connected. At the same time, the regulating valve 7 on the second pipe 10 corresponding to partition 16 is opened, and multiple sets of culture medium delivery components 3 deliver culture medium to the flow box 1, and mix it through mixing component 8. Finally, it is discharged into culture container 2 through second pipe 10. The overall structure is simple to operate, has multiple mixing mechanisms, improves the mixing uniformity of various culture media, and ensures the quality and efficiency of cell culture. Multiple sets of culture medium delivery components 3 support the simultaneous delivery of culture media with different components to meet complex culture needs. At the same time, the modular setting between multiple structures makes it easy to replace or upgrade independently.

[0044] This utility model is not limited to the above-described embodiments. Anyone should know that structural changes made under the guidance of this utility model, and any technical solutions that are the same as or similar to this utility model, fall within the protection scope of this utility model. Technical aspects, shapes, and structures not described in detail in this utility model are all publicly known technologies.

Claims

1. A modular cell culture tubing system, characterized in that, include: The top of the flow guide box (1) is connected to multiple culture medium delivery components (3) through a first pipe (6), and the bottom of the flow guide box (1) is connected to multiple mixing components (8). A partition (16) runs through and is movably connected inside the flow guide box (1). The partition (16) divides the inside of the flow guide box (1) into a first chamber (14) and a second chamber (15). The flow guide box (1) is provided with an adjustment component (9) for adjusting the partition (16). The culture container (2) is connected to multiple mixing components (8) via multiple second pipes (10), and a regulating valve (7) is connected between the second pipes (10) and the mixing components (8).

2. The modular cell culture tubing system according to claim 1, characterized in that, The mixing assembly (8) includes a mixing pipe (81) and a mixing plate (82). The mixing pipe (81) is connected to the guide box (1) and the regulating valve (7). The mixing plate (82) is fixedly installed inside the mixing pipe (81). The mixing plate (82) has a spiral structure and forms a spiral channel inside the mixing pipe (81).

3. The modular cell culture tubing system according to claim 2, characterized in that, A flow-guiding funnel (12) is connected between the mixing pipe (81) and the flow guide box (1).

4. A modular cell culture tubing system according to claim 2, characterized in that, The adjustment component (9) includes: Mounting bracket (92) is fixedly mounted on the flow guide box (1). A threaded rod (91) is provided through and movable on the mounting bracket (92). The threaded rod (91) is rotatably mounted on the top of the partition plate (16). A worm gear (93) is sleeved and threadedly connected to the threaded rod (91). The worm gear (93) is rotatably mounted on the mounting bracket (92). A worm (94) that meshes with the worm gear (93) is rotatably connected to the mounting bracket (92).

5. A modular cell culture tubing system according to claim 4, characterized in that, The partition (16) has a bonding plate (17) fixedly connected to both ends, and a sealing gasket (18) is fixedly installed on the bonding plate (17).

6. A modular cell culture tubing system according to claim 1, characterized in that, A third pipe (11) is connected between the first pipe (6) and the flow guide box (1), and the third pipe (11) is detachably connected to the first pipe (6) through a Luer connector (5).

7. A modular cell culture tubing system according to claim 6, characterized in that, A one-way valve (4) is connected between the third pipe (11) and the flow guide box (1).

8. A modular cell culture tubing system according to claim 6, characterized in that, The guide box (1) is detachably connected to a cover (13).

Images