Device for continuously culturing fresh water microalgae indoors at constant temperature

Abstract

The utility model provides a device for continuously culturing fresh water microalgae indoors at constant temperature. According to the device, a cross beam and a hanging frame are installed at the top of each main frame, breeding bags are correspondingly hung on the two sides of each hanging frame, a pH value detector and an air inlet pipe are arranged in each breeding bag, a monitoring alarm box is installed on the main frame at the end, and an upper light bar and a lower light bar are installed on the two sides of each main frame and correspond to each breeding bag. Sewage discharge grooves are formed in the ground at the two outer ends of the main frame, the bottom of each culture bag is communicated with a liquid conveying mechanism, and an algae liquid conveying mechanism is arranged on the ground at the inner end of the main frame. The buffer tank is provided with a non-contact liquid level monitor, a material pipe, a material pipe electromagnetic valve and a buffer tank electromagnetic valve; and the main conveying pipe is provided with a segmented electromagnetic valve, a connecting straight pipe and a branch pipe electromagnetic valve. According to the device, the culture bags are connected in series to culture the freshwater microalgae, the liquid conveying mechanism is used for unified conveying, the structure is simple and practical, the automation degree is high, the equipment investment cost is low, and the yield of the freshwater microalgae is high.

Description

Technical Field

[0001] This utility model relates to the field of microalgae cultivation equipment technology, and in particular to a device for indoor constant temperature continuous cultivation of freshwater microalgae. Background Technology

[0002] Microalgae are tiny, single-celled algae that typically exist in single-celled or multicellular forms and grow in water. Microalgae cultivation is an emerging agricultural aquaculture model that has attracted considerable attention due to its environmental friendliness, high efficiency, and sustainability. Its main characteristics include rapid growth and rich content of nutrients such as protein, fat, carbohydrates, and various vitamins, making it widely used in food, feed, cosmetics, and pharmaceuticals. Freshwater microalgae cultivation is water-based, and its growth requires comprehensive consideration of factors such as light, temperature, nutrients, pH, and the cultivation system. Different species and environments require targeted optimization of conditions. Most freshwater microalgae grow optimally at around 24-26℃; excessively low or high temperatures will adversely affect their growth. Therefore, temperature control and appropriate adjustments are necessary during freshwater microalgae cultivation. Freshwater microalgae are quite sensitive to pH levels. Maintaining a suitable pH during cultivation is crucial; excessively high or low pH levels will negatively impact their growth. The optimal pH range for freshwater microalgae growth is 7.0-8.5. In artificial cultivation, pH levels may fluctuate due to nutrient absorption, metabolism, or changes in carbon dioxide concentration. Stabilization requires adjusting the amount of mixed gas (containing 1%-5% carbon dioxide) or the composition of the nutrient solution. Freshwater microalgae have high light requirements; generally, strong light is beneficial for photosynthesis and growth. There is a relationship between the density of freshwater microalgae and light intensity. Excessive density or insufficient light intensity will lead to competition and insufficient photosynthesis. Therefore, it is essential to control the cultivation density and light intensity to ensure normal growth. When cultivating freshwater microalgae, an environment with moderate light intensity should be selected and appropriately controlled. The light duration is generally 12-16 hours, and the light source can be sunlight or artificial light. Freshwater microalgae require ample nutrients for normal growth. Commonly used nutrients include nitrogen, phosphorus, and potassium, which can be provided by adding appropriate amounts of compound fertilizer or other specialized fertilizers. In addition, some trace elements and organic matter can be added to promote their growth. Freshwater microalgae are susceptible to pollution and pests; therefore, maintaining cleanliness and hygiene is crucial during cultivation to prevent external contamination and pest infestations. Regular cleaning of cultivation equipment, pipelines, and filtration solutions is essential, along with thorough disinfection. In summary, freshwater microalgae cultivation is a promising aquaculture model, but careful attention must be paid to light, temperature, nutrient supply, and water quality monitoring to maintain a stable growth environment. Simultaneously, preventing disease infection and pest outbreaks is vital, requiring regular cleaning of equipment, disinfection of the water, and maintenance of the cultivation equipment's cleanliness.

[0003] Currently, most existing technologies for the artificial cultivation of freshwater microalgae utilize closed glass tubes outdoors. This requires suitable temperatures; for example, Haematococcus pluvialis thrives at around 25 degrees Celsius. In Yunnan, the temperature difference is small throughout the year, and the temperature remains around 25 degrees Celsius for most of the time. Therefore, in some areas of Yunnan, Haematococcus pluvialis is cultivated outdoors using closed glass tubes. Because outdoor temperatures cannot be kept completely constant, water circulation is also used to regulate the temperature in Yunnan. While closed glass tubes effectively control the environment, the installation of these tubes is complex, requires a large area, and necessitates the use of numerous pumps, resulting in high equipment and operating costs for this cultivation method.

[0004] Another method for outdoor cultivation of freshwater microalgae involves constructing open cultivation ponds on the ground, adding culture medium to the ponds, and controlling the temperature of the microalgae cultivated in these ponds. Although the cost of cultivation ponds is relatively low, the external environment cannot be artificially controlled. Furthermore, the microalgae cultivated in these ponds are susceptible to contamination by bacteria and protozoa, which significantly harms their growth. Additionally, the yield of microalgae cultivated in these ponds is not high.

[0005] Another indoor cultivation method for freshwater microalgae is to use transparent plastic bags for hanging cultivation, where the algal solution is transported into the transparent plastic bags for cultivation. Although this method has lower equipment costs and allows for effective control of the cultivation environment, the cultivation bags are independent of each other and are only suitable for small-scale freshwater microalgae cultivation. Generally, this method is only used for cultivating algal strains.

[0006] In summary, to address the problems existing in the prior art, the technicians of this application have designed a device for continuous indoor constant-temperature cultivation of freshwater microalgae. Summary of the Invention

[0007] The purpose of this invention is to provide a device for continuous indoor constant-temperature cultivation of freshwater microalgae. The technical problem this invention aims to solve is: to cultivate freshwater microalgae indoors at a constant temperature using a series of connected cultivation bags, while simultaneously using a buffer tank, main delivery pipe, and connecting branch pipes to uniformly deliver algal solution and nutrient solution to each cultivation bag; to monitor the pH value within the cultivation bags using a pH meter; and to simulate sunlight irradiation of the algal solution within the cultivation bags using light emitted from a light strip, thus solving the technical problem of large-scale indoor cultivation of freshwater microalgae.

[0008] To achieve the above objectives, the technical solution of this utility model is as follows:

[0009] An apparatus for continuous indoor constant-temperature cultivation of freshwater microalgae includes: a main frame, cultivation bags, a pH meter, a monitoring and alarm box, a sewage discharge solenoid valve, a sewage discharge trough, crossbeams, hanging racks, upper light strips, lower light strips, an air inlet pipe, a visitor walkway, a breeding room, a central control room, a disinfection and sterilization room, a personnel changing room, a central air conditioning room, a gas mixing room, a liquid conveying mechanism, and an algae liquid conveying mechanism. The liquid conveying mechanism includes: a main conveying pipe, connecting straight pipes, branch pipe solenoid valves, segmented solenoid valves, a support frame, a buffer tank solenoid valve, a buffer tank, a feed pipe solenoid valve, a feed pipe, a contactless liquid level monitor, and a liquid mixing room. The algae liquid conveying mechanism includes: an algae liquid collection tank, a discharge solenoid valve, a liquid pump, and a collection tank. The apparatus has horizontal beams mounted on the top of each main frame. The beams and crossbeams are fixedly mounted with racks at intervals. Culture bags are suspended on both sides of each rack. A pH meter is installed inside each culture bag closest to the end main frame. A monitoring and alarm box is installed on the end main frame, and the pH meter is connected to the monitoring and alarm box via a wire. An air inlet pipe is installed on the lower side of each culture bag, connecting to the output pipe of the gas mixing chamber. Upper and lower light strips are installed on both sides of each main frame corresponding to each culture bag. Drainage troughs are installed on the ground at both outer ends of the main frame, with a solenoid valve installed on the main delivery pipe above the troughs. The troughs are connected to a wastewater treatment system. The bottom of each culture bag is connected to a liquid delivery mechanism. An algae liquid delivery mechanism is installed on the ground at the inner end of the main frame.

[0010] The liquid conveying mechanism in the device has a buffer tank at the end of the main frame. A bracket is welded to the bottom of the buffer tank. A non-contact liquid level monitor is installed on one side of the buffer tank. A feed pipe is installed on the top of the buffer tank, and a feed pipe solenoid valve is installed on the feed pipe. Each feed pipe is connected to the corresponding output pipe of the liquid mixing room. A buffer tank solenoid valve is installed on the main conveying pipe at the bottom of the buffer tank. A segment solenoid valve is installed on the horizontal section of the main conveying pipe. A connecting straight pipe is installed on the horizontal section of the main conveying pipe at the position of each breeding bag. A branch pipe solenoid valve is installed on each connecting straight pipe. Each connecting straight pipe is connected to the bottom of the corresponding breeding bag.

[0011] The algae liquid conveying mechanism in the device has an algae liquid collection tank set on the ground at the inner end of the main frame. A discharge solenoid valve is installed on the discharge pipe above the algae liquid collection tank. The end of the algae liquid collection tank is connected to the collection tank. The liquid output pipe of the collection tank is connected to the input pipe of the liquid pump. The output pipe of the liquid pump is connected to the next process.

[0012] The main frame of the device is installed according to the area of ​​the breeding workshop. A walkway is left between each row of main frames. A visitor walkway is set on one side of the breeding workshop. A breeding room is set at one end of the breeding workshop. A disinfection and sterilization room, a central control room and a personnel changing room are set outside the breeding room in sequence. A central air conditioning room is set on one side of the personnel changing room. A gas mixing room and a liquid feeding room are set on one side of the central air conditioning room in sequence. An air compressor and a carbon dioxide storage tank are installed in the gas mixing room. The mixed gas output pipe of the gas mixing room is connected to the air inlet pipe. Each liquid output pipe of the liquid feeding room is connected to the corresponding feed pipe.

[0013] The monitoring and alarm box, sewage solenoid valve, branch pipe solenoid valve, segment solenoid valve, discharge solenoid valve, buffer tank solenoid valve, material pipe solenoid valve, non-contact liquid level monitor, upper light bar, lower light bar, liquid pump, central air conditioning room, and gas mixing chamber in the device are respectively connected to the electrical control unit in the central control room by wires.

[0014] All solenoid valves, liquid pumps, and buffer tanks involved in the device are mature products in existing technology. The monitoring and alarm box is a product manufactured by Hangzhou Liance Automation Technology Co., Ltd., and the non-contact liquid level monitor is a product manufactured by Shenzhen Xingkechuang Technology Co., Ltd.

[0015] Compared with the prior art, the positive effects of this utility model are as follows:

[0016] 1. This device uses a series of culture bags to cultivate freshwater microalgae in a constant-temperature indoor environment. At the same time, a liquid delivery mechanism is used to uniformly deliver algal solution and nutrient solution to each culture bag, thus solving the technical problem of large-scale indoor cultivation of freshwater microalgae.

[0017] 2. The liquid conveying mechanism in this device includes a liquid mixing room, a buffer tank, a main conveying pipe, and connecting branch pipes. After the nutrient solution is prepared in proportion in the liquid mixing room, it is conveyed to the buffer tank through the pipeline. The nutrient solution is mixed in the buffer tank. The buffer tank utilizes the height difference between itself and the breeding bags to uniformly convey the nutrient solution to each breeding bag under the action of gravity. The nutrient solutions can be mixed before conveying, or a certain nutrient solution can be conveyed separately.

[0018] 3. Each culture bag in the device is equipped with an air inlet pipe on one side, which is connected to the gas mixing chamber. The mixed gas with added carbon dioxide prepared in the gas mixing chamber enters the culture bag through the air inlet pipe, providing the necessary carbon source for freshwater microalgae during their growth period.

[0019] 4. The device is equipped with a pH meter in the first culture bag of freshwater microalgae in each row of the same culture period. The pH meter is used to monitor the changes in pH value in the culture bag and adjust the pH value in the culture bag by increasing or decreasing the carbon dioxide content in the mixed gas.

[0020] 5. Upper and lower light strips are installed on both sides of each main frame. By turning some of the light strips on or off, the light required by freshwater microalgae at different growth stages can be provided to ensure photosynthesis during the growth stages of microalgae.

[0021] 6. The indoor temperature of the device is controlled and regulated by the operators in the central control room by adjusting the central air conditioning to ensure that the overall temperature of the aquaculture workshop is within the suitable temperature range for the growth of freshwater microalgae.

[0022] 7. The freshwater microalgae that have completed cultivation in the culture bags in this device are collected and discharged into the collection tank through the algae liquid collection tank. The algae liquid in the collection tank is then transported to the next process for further processing using a liquid pump.

[0023] 8. Wastewater from the disinfection and cleaning process of this device is discharged into the wastewater treatment system through a sewage discharge trough. The wastewater is then centrally treated by the wastewater treatment system, and after meeting the standards, it is either recycled or discharged.

[0024] 9. The device has a simple and practical structure, a high degree of automation, and is very easy to use for unified or segmented cultivation. The equipment investment cost is low, and the yield of freshwater microalgae is high, realizing large-scale constant-temperature cultivation of freshwater microalgae indoors. Attached Figure Description

[0025] To more clearly illustrate the technology of this utility model in its embodiments, the accompanying drawings are briefly introduced below. The drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without any creative effort.

[0026] The accompanying drawings, including their structure, proportions, and sizes, are only intended to complement the content disclosed in this specification and to enable those skilled in the art to understand and read them. They are not intended to limit the conditions under which this utility model can be implemented. Any modifications to the structure, changes in proportions, or adjustments to the size, without affecting the effects and objectives that this utility model can produce, should still fall within the scope of the technical content disclosed in this utility model.

[0027] Figure 1 A schematic diagram of the main structure of a freshwater microalgae cultivation device;

[0028] Figure 2 A side view of a freshwater microalgae cultivation device;

[0029] Figure 3 A schematic diagram of the workshop structure for freshwater microalgae cultivation;

[0030] Figure 4 A diagram showing the connection between each component and the central control room.

[0031] In the diagram: 1. Main frame, 2. Culture bag, 3. pH meter, 4. Monitoring box, 5. Sewage discharge solenoid valve, 6. Sewage discharge trough, 7. Main delivery pipe, 8. Connecting straight pipe, 9. Branch pipe solenoid valve, 10. Segmented solenoid valve, 11. Algae liquid collection tank, 12. Discharge solenoid valve, 13. Support frame, 14. Buffer tank solenoid valve, 15. Buffer tank, 16. Feed pipe solenoid valve, 17. Feed pipe, 18. Non-contact liquid level monitor, 19. Crossbeam, 20. Hanging rack, 21. Upper light strip, 22. Lower light strip, 23. Air inlet pipe, 24. Visitor walkway, 25. Liquid pump, 26. Collection tank, 27. Breeding room, 28. Central control room, 29. Disinfection and sterilization room, 30. Personnel changing room, 31. Central air conditioning room, 32. Liquid mixing room, 33. Gas mixing room. Detailed Implementation

[0032] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings and embodiments. The specific embodiments described below are only used to further illustrate this application and should not be construed as limiting the scope of protection of this application. Those skilled in the art can make some non-essential improvements and adjustments to this application based on the above application content.

[0033] See appendix Figure 1-4 The installation and connection of the device are as follows: The number of main frames 1 to be installed is calculated based on the floor area of ​​the breeding workshop. The main frames 1 are installed in two rows, with N rows of main frames 1 in each row. A walkway is left between the two rows of main frames 1 and each row of main frames 1. Crossbeams 19 are installed on the top of each main frame 1, connecting them into a whole. Hangers 20 are fixed to the crossbeams 19 at intervals. Breeding bags 2 are suspended on both sides of each hanger 20, and the bottom of each breeding bag 2 is connected to the liquid conveying mechanism. A pH meter 3 is installed inside the breeding bag 2 close to the end main frame 1. A monitoring alarm box 4 is installed on the end main frame 1, and the pH meter 3 is connected to the monitoring alarm box 4 via a wire. Air inlet pipes 23 are respectively installed on the lower side of each breeding bag 2, and are connected to the output pipe of the gas mixing chamber 33. Upper light strips 21 and lower light strips 22 are respectively installed on both sides of each main frame 1, corresponding to each breeding bag 2. The sewage discharge trough 6 is set on the ground at both outer ends of the main frame 1. The sewage discharge solenoid valve 5 is installed on the main conveying pipe 7 above the sewage discharge trough 6. The sewage discharge trough 6 is connected to the sewage treatment system. The algae liquid conveying mechanism is set on the ground at the inner end of the main frame 1.

[0034] A buffer tank 15 is located at the end of the main frame 1, and a bracket 13 is welded to the bottom of the buffer tank 15. A contactless liquid level monitor 18 is installed on one side of the buffer tank 15, and a feed pipe 17 is installed on the top of the buffer tank 15. A feed pipe solenoid valve 16 is installed on the feed pipe 17, and each feed pipe 17 is connected to the corresponding output pipe of the liquid mixing room 32. A buffer tank solenoid valve 14 is installed on the main conveying pipe 7 at the bottom of the buffer tank 15, a segmented solenoid valve 10 is installed on the horizontal section of the main conveying pipe 7, and connecting straight pipes 8 are installed at the position of each breeding bag 2 corresponding to the horizontal section of the main conveying pipe 7. A branch pipe solenoid valve 9 is installed on each connecting straight pipe 8, and each connecting straight pipe 8 is connected to the bottom of the corresponding breeding bag 2.

[0035] The algae liquid collection tank 11 is set on the ground at the inner end of the main frame 1. The discharge solenoid valve 12 is installed on the discharge pipe above the algae liquid collection tank 11. The end of the algae liquid collection tank 11 is connected to the collection tank 26. The liquid output pipe of the collection tank 26 is connected to the input pipe of the liquid pump 25. The output pipe of the liquid pump 25 is connected to the next process.

[0036] The visitor walkway 24 is located on one side of the breeding workshop, the breeding room 27 is located at one end of the breeding workshop, the central control room 28 is located outside the partition wall of the breeding room 27, the disinfection and sterilization room 29 and the personnel changing room 30 are located on both sides of the central control room 28, the central air conditioning room 31 is located on one side of the personnel changing room 30, the gas mixing room 33 is located on one side of the central air conditioning room 31, and the liquid feed preparation room 32 is located on one side of the gas mixing room 33. An air compressor and a carbon dioxide storage tank are installed inside the gas mixing room 33. The mixed gas output pipe of the gas mixing room 33 is connected to the air inlet pipe 23, and each liquid output pipe of the liquid feed preparation room 32 is connected to the corresponding feed pipe 17.

[0037] Number the drain solenoid valve 5, branch pipe solenoid valves 9, segment solenoid valves 10, discharge solenoid valve 12, buffer tank solenoid valve 14, and feed pipe solenoid valve 16 in the device, and then connect them to the electrical control unit in the central control room 28 with wires. Connect the monitoring alarm box 4, contactless liquid level monitor 18, upper light strip 21, lower light strip 22, liquid pump 25, central air conditioning room 31, and gas mixing chamber 33 to the electrical control unit in the central control room 28 with wires. Example 1

[0038] The overall cultivation of freshwater microalgae in the aquaculture workshop

[0039] The number of main frames 1 to be installed is calculated based on the floor area of ​​the breeding workshop. The main frames 1 are installed in two columns, with N rows of main frames 1 in each column. A walkway is left between the two columns of main frames 1 and each row of main frames 1. Crossbeams 19 are installed on the top of each main frame 1, connecting the main frames 1 into a whole. Hangers 20 are fixed to the crossbeams 19 at intervals. The breeding bags 2 are correspondingly suspended on both sides of each hanger 20. The bottom of each breeding bag 2 is connected to the liquid conveying mechanism. A pH meter 3 is installed in the breeding bag 2 close to the end main frame 1. A monitoring and alarm box 4 is installed on the end main frame 1, and the pH meter 3 is connected to the monitoring and alarm box 4 through a wire. Air inlet pipes 23 are respectively installed on the lower side of each breeding bag 2, and the air inlet pipes 23 are connected to the output pipe of the gas mixing chamber 33. The upper light strip 21 and lower light strip 22 are respectively installed on both sides of each main frame 1, and each breeding bag 2 is corresponding to the upper light strip 21 and lower light strip 22. Each feed pipe 17 is connected to the corresponding output pipe of the liquid mixing room 32. The sewage discharge solenoid valve 5, each branch pipe solenoid valve 9, the segment solenoid valve 10, the discharge solenoid valve 12, the buffer tank solenoid valve 14, and the feed pipe solenoid valve 16 are numbered and then connected to the electrical control unit in the central control room 28 with wires. The monitoring alarm box 4, the non-contact liquid level monitor 18, the upper light strip 21, the lower light strip 22, the liquid pump 25, the central air conditioning room 31, and the gas mixing room 33 are respectively connected to the electrical control unit in the central control room 28 with wires. Before use, the process parameters of the monitoring alarm box 4 and the non-contact liquid level monitor 18, the temperature parameters of the central air conditioning in the central air conditioning room 31, and the parameters of carbon dioxide and air mixing in the gas mixing room 33 are respectively input into the electrical control unit in the central control room 28.

[0040] In operation, the algae spores cultivated in the breeding room 27 are first transferred to the buffer tank 15. Simultaneously, the operator opens the section solenoid valve 10 and the buffer tank solenoid valve 14 via the central control room 28, and then opens a branch solenoid valve 9. Utilizing the height difference between the buffer tank 15 and the culture bag 2, the algae spores are transferred in the correct amount via the main delivery pipe 7 and the connecting straight pipe 8 into the culture bag 2. Then, the branch solenoid valve 9 on the connecting straight pipe 8 is closed. This process is repeated to transfer the algae spores to each culture bag 2 in the correct amount. After the algae spore transfer is complete, the operator opens each branch solenoid valve 9 via the central control room 28. Based on the amount of nutrient solution required for the growth of freshwater microalgae, the nutrient solutions prepared in the liquid preparation room 32 are transferred in proportion via the feed pipe 17 and the feed pipe solenoid valve 16 into the buffer tank 15. Utilizing the height difference between the buffer tank 15 and the culture bag 2, the nutrient solutions are then transferred to each culture bag 2 via the main delivery pipe 7 and the connecting straight pipe 8. Simultaneously, the mixture of carbon dioxide and air in the gas mixing chamber 33 is delivered to each culture bag 2 through the air inlet pipe 23, providing the necessary carbon source for the growth of freshwater microalgae. When the contactless liquid level monitor 18 detects that the buffer tank 15 and the culture bag 2 have reached the same liquid level, the operator closes the buffer tank solenoid valve 14 and the solenoid valves 9 of each branch pipe through the central control room 28 to stop the delivery of nutrient solution. Depending on the required sunlight intensity for the freshwater microalgae, the upper light bar 21 or the lower light bar 22 is turned on. When the freshwater microalgae require strong light for growth, both the upper light bar 21 and the lower light bar 22 are turned on simultaneously to ensure the light required for photosynthesis.

[0041] When the pH meter 3 inside the breeding bag 2 detects a pH value exceeding 7.0-8.5, the monitoring alarm box 4 issues an audible and visual alarm. Operators then adjust the amount of carbon dioxide added to the air via the central control room 28 to ensure the pH value inside the breeding bag 2 remains within the 7.0-8.5 range. Similarly, when the temperature inside the breeding workshop is higher or lower than the set value, the monitoring alarm box 4 also issues an audible and visual alarm. Operators then adjust the output temperature of the central air conditioning system in the central air conditioning room 31 via the central control room 28 to maintain the optimal temperature inside the breeding workshop.

[0042] Once the freshwater microalgae cultivation in the culture bag 2 is completed, the operator opens the solenoid valves 9 of each branch pipe and the discharge solenoid valve 12 through the central control room 28. The freshwater microalgae in each culture bag 2 are transported to the algae liquid collection tank 11 through the connecting straight pipe 8 and the main conveying pipe 7. The algae liquid enters the collection tank 26 along the algae liquid collection tank 11, and is then transported to the next process for treatment by the liquid pump 25.

[0043] When each breeding bag 2, main conveying pipe 7, connecting straight pipe 8, and buffer tank 15 needs to be cleaned and disinfected, disinfectant and clean water are injected in sequence to disinfect and clean the above components. Then, the sewage discharge solenoid valve 5 is opened, and the sewage is discharged into the sewage discharge tank 6. The sewage is then discharged into the sewage treatment system through the sewage discharge tank 6 for centralized treatment.

[0044] When operators enter the breeding workshop, they must change clothes in the personnel changing room 30 before entering the breeding workshop. The disinfection and sterilization room 29 is used to disinfect and sterilize the clothes, hats, slippers, and utensils removed after work. When external personnel visit, they can only visit along the visitor corridor 14 to avoid bringing external germs into the workshop, thus ensuring the safety and hygiene of the breeding workshop. Example 2

[0045] The situation of segmented cultivation of freshwater microalgae in the aquaculture workshop

[0046] The main frame 1, the arrangement of the breeding bags, the pH meter 3, the monitoring and alarm box 4, the buffer tank 15, the arrangement of each pipeline, and the installation of each solenoid valve are all the same as in Example 1, and will not be described again.

[0047] In operation, the algae spores cultivated in breeding room 27 are first transferred to buffer tank 15. Simultaneously, the operator opens the section solenoid valve 10 and buffer tank solenoid valve 14 via the central control room 28. Then, one branch solenoid valve 9 within the section is opened, while the branch solenoid valve 9 outside the section is closed. Utilizing the height difference between buffer tank 15 and culture bag 2, the algae spores are transferred in the correct amount through the main delivery pipe 7 and connecting straight pipe 8 into the culture bag 2. Then, the branch solenoid valve 9 on the connecting straight pipe 8 is closed. This process is repeated to transfer the algae spores in the correct amount into each culture bag 2 within the section. The subsequent nutrient solution input, mixed gas delivery, lighting, algae liquid collection, and wastewater discharge operations are the same as in Example 1 and will not be repeated. The culture bags 2 outside the sections are operated according to the above-described procedures for algae spore input, nutrient solution input, mixed gas delivery, lighting, algae liquid collection, and wastewater discharge.

[0048] The above description is only a non-limiting embodiment of the present utility model, and a large number of embodiments can be derived. For those skilled in the art, without departing from the inventive concept of the present utility model and without making creative efforts, several modified and improved embodiments can be made, all of which fall within the protection scope of the present utility model.

Claims

1. An apparatus for indoor constant-temperature continuous cultivation of freshwater microalgae, comprising: Main frame (1), culture bag (2), pH meter (3), monitoring alarm box (4), sewage discharge solenoid valve (5), sewage discharge trough (6), crossbeam (19), hanging rack (20), upper light strip (21), lower light strip (22), air inlet pipe (23), visitor walkway (24), breeding room (27), central control room (28), disinfection and sterilization room (29), personnel changing room (30), central air conditioning room (31), gas mixing room (33), liquid conveying mechanism, algae liquid conveying mechanism, among which liquid The body conveying mechanism includes: a main conveying pipe (7), a connecting straight pipe (8), a branch pipe solenoid valve (9), a segmented solenoid valve (10), a support (13), a buffer tank solenoid valve (14), a buffer tank (15), a feed pipe solenoid valve (16), a feed pipe (17), a non-contact liquid level monitor (18), and a liquid batching room (32). The algae liquid conveying mechanism includes: an algae liquid collection tank (11), a discharge solenoid valve (12), a liquid pump (25), and a collection tank (26). The device is characterized in that: the device is located on each main frame (1) A crossbeam (19) is installed on the top of the structure. Hangers (20) are fixed on the crossbeam (19) at intervals. A culture bag (2) is suspended on both sides of each hanger (20). A pH meter (3) is installed in the culture bag (2) close to the end main frame (1). A monitoring alarm box (4) is installed on the end main frame (1). The pH meter (3) is connected to the monitoring alarm box (4) through a wire. An air inlet pipe (23) is installed on the lower side of each culture bag (2). The air inlet pipe (23) is connected to the air supply pipe. The output pipes of the mixing chamber (33) are connected. Each main frame (1) is equipped with an upper light strip (21) and a lower light strip (22) on both sides corresponding to each culture bag (2). Drainage troughs (6) are set on the ground at both outer ends of the main frame (1). A drain solenoid valve (5) is installed on the main conveying pipe (7) above the drainage trough (6). The drainage trough (6) is connected to the sewage treatment system. The bottom of each culture bag (2) is connected to the liquid conveying mechanism. An algae liquid conveying mechanism is set on the ground at the inner end of the main frame (1).

2. The device for indoor constant-temperature continuous cultivation of freshwater microalgae according to claim 1, characterized in that: The liquid conveying mechanism in the device has a buffer tank (15) at the end of the main frame (1). A bracket (13) is welded to the bottom of the buffer tank (15). A non-contact liquid level monitor (18) is installed on one side of the buffer tank (15). A feed pipe (17) is installed on the top of the buffer tank (15). A feed pipe solenoid valve (16) is installed on the feed pipe (17). Each feed pipe (17) is connected to the corresponding output pipe of the liquid mixing room (32). A buffer tank solenoid valve (14) is installed on the main conveying pipe (7) at the bottom of the buffer tank (15). A segment solenoid valve (10) is installed on the main conveying pipe (7) in the horizontal section. A connecting straight pipe (8) is installed on the main conveying pipe (7) in the horizontal section corresponding to the position of each breeding bag (2). A branch pipe solenoid valve (9) is installed on each connecting straight pipe (8). Each connecting straight pipe (8) is connected to the bottom of the corresponding breeding bag (2).

3. The device for indoor constant-temperature continuous cultivation of freshwater microalgae according to claim 1, characterized in that: The algae liquid conveying mechanism in the device has an algae liquid collection tank (11) set on the ground at the inner end of the main frame (1). A discharge solenoid valve (12) is installed on the discharge pipe above the algae liquid collection tank (11). The end of the algae liquid collection tank (11) is connected to the collection tank (26). The liquid output pipe of the collection tank (26) is connected to the input pipe of the liquid pump (25). The output pipe of the liquid pump (25) is connected to the next process.

4. The device for indoor constant-temperature continuous cultivation of freshwater microalgae according to claim 1, characterized in that: The installation of the main frame (1) in the device is set according to the area of ​​the breeding workshop. A walkway is left between each row of main frames (1). A visitor walkway (24) is set on one side of the breeding workshop. A breeding room (27) is set at one end of the breeding workshop. A disinfection and sterilization room (29), a central control room (28) and a personnel changing room (30) are set in sequence outside the breeding room (27). A central air conditioning room (31) is set on one side of the personnel changing room (30). A gas mixing room (33) and a liquid feeding room (32) are set in sequence on one side of the central air conditioning room (31). An air compressor and a carbon dioxide storage tank are installed in the gas mixing room (33). The mixed gas output pipe of the gas mixing room (33) is connected to the air inlet pipe (23). Each liquid output pipe of the liquid feeding room (32) is connected to the corresponding feed pipe (17).

5. The device for indoor constant-temperature continuous cultivation of freshwater microalgae according to claim 1, characterized in that: The monitoring alarm box (4), sewage discharge solenoid valve (5), branch pipe solenoid valve (9), segment solenoid valve (10), discharge solenoid valve (12), buffer tank solenoid valve (14), material pipe solenoid valve (16), non-contact liquid level monitor (18), upper light bar (21), lower light bar (22), liquid pump (25), central air conditioning room (31), and gas mixing room (33) in the device are respectively connected to the electrical control unit in the central control room (28) by wires.

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