Exosome extraction device

Abstract

The utility model discloses an exosome extracting device which comprises a collecting pipe, an ultrafiltration inner pipe and a pipe cover, the ultrafiltration inner pipe is clamped in a pipe body of the collecting pipe, the pipe cover is installed at the top end of the collecting pipe, the exosome extracting device further comprises a lining film mechanism, the lining film mechanism comprises supporting ribs, supporting ribs, opposite lining plates, a middle lining plate, a lifting ring and lining claw strips, and the supporting ribs are connected with the supporting ribs. A lining membrane mechanism is arranged in a collecting pipe of an ultrafiltration centrifugal pipe, supporting ribs and supporting ribs of the lining membrane mechanism are arranged in the collecting pipe to support and clamp a counter lining plate and a middle lining plate, and a bottom block body of an ultrafiltration inner pipe can penetrate through a lifting ring, so that a lower filter membrane is supported and connected by the middle lining plate and the counter lining plate; the lining plate and the middle lining plate are provided with the lining claw strips to support the ultrafiltration membrane of the ultrafiltration inner pipe, the deformation degree of the ultrafiltration membrane is effectively limited, breakage caused by excessive deformation of the membrane body is avoided, the success rate of exosome extraction is increased, and the quality of exosome is guaranteed.

Description

Technical Field

[0001] This utility model relates to the field of exosome extraction technology, and in particular to an exosome extraction device. Background Technology

[0002] Exosomes are membrane vesicles with a diameter of approximately 30-150 nm secreted by living cells. They are naturally present in body fluids such as blood, saliva, urine, and breast milk. In laboratory research, ultrafiltration is a widely accepted and relatively simple method. Ultrafiltration typically uses ultrafiltration centrifuge tubes, which usually consist of a collection tube, a cap, and an inner ultrafiltration tube containing an ultrafiltration membrane. The biological sample containing exosomes is added to the inner ultrafiltration tube of the ultrafiltration centrifuge tube, and then the ultrafiltration centrifuge tube is placed in a centrifuge device. Centrifugation forces the liquid in the sample through the ultrafiltration membrane, while the exosomes, due to their larger particle size, are retained above the ultrafiltration membrane and removed by a pipette, thus achieving the separation of exosomes from other small molecules.

[0003] In existing technologies, when extracting exosomes using ultrafiltration centrifuge tubes, the ultrafiltration inner tube of the collection tube is filled with a biological sample containing exosomes and the tube is capped. The filter membrane in this type of ultrafiltration inner tube is axially symmetrical. As the ultrafiltration centrifuge tube is placed at different angles in the centrifuge equipment, the two membranes of the filter membrane are subjected to different pressures, which may cause one set of membranes to be subjected to greater pressure and break, thus affecting the extraction of exosomes. To address this, we propose an exosome extraction device. Utility Model Content

[0004] The main objective of this invention is to provide an exosome extraction device. A membrane liner mechanism is installed inside the collection tube of an ultrafiltration centrifuge tube. The liner mechanism's support strips and struts are positioned inside the collection tube to support and hold the liner plate and the middle liner plate. The bottom block of the ultrafiltration inner tube can pass through a lifting ring, allowing the lower filter membrane to be supported by the middle and liner plates. During the exosome extraction centrifugation process, the liner plate and the middle liner plate, along with the liner claws, support the ultrafiltration membrane of the ultrafiltration inner tube, effectively limiting the degree of deformation of the ultrafiltration membrane, preventing rupture due to excessive membrane deformation, improving the success rate of exosome extraction, and ensuring the quality of the exosomes. This effectively solves the problems in the prior art.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] An exosome extraction device includes a collection tube, an ultrafiltration inner tube, and a cap. The ultrafiltration inner tube is fitted inside the collection tube, and the cap is installed at the top of the collection tube. The device also includes a membrane lining mechanism, which includes support strips, ribs, a liner plate, a middle liner plate, a lifting ring, and liner claws. Support strips are symmetrically protruding inside the collection tube below the ultrafiltration inner tube, and the liner plates and the middle liner plate are supported on the surface of the support strips for lining the filter membrane below the ultrafiltration inner tube. Support strips protrude from the top of the support strips, and the liner plates and the middle liner plate are fitted between the ribs. A lifting ring is integrally formed at the top of the liner plates and the middle liner plate, and liner claws symmetrically protrude from the inclined surface of the liner plates and the middle liner plate towards the ultrafiltration inner tube.

[0007] Furthermore, the strip of the liner claw is integrally formed on the body of the liner plate and the middle liner plate, and the strip of the liner claw has branch bars protruding outwards.

[0008] By adopting the above technical solution, the filter membrane below the ultrafiltration inner tube can be supported by the strip discs and branch bars of the lining claw strips and the middle lining plate.

[0009] Furthermore, the four sets of plates of the liner are integrally formed below the ring of the lifting ring, and a middle liner is fixed below the lifting ring between every two sets of connected plates of the liner.

[0010] By adopting the above technical solution, the four sets of plates below the lifting ring, together with the two sets of plates of the middle liner, can provide support for the filter membrane below the ultrafiltration inner tube.

[0011] Furthermore, the top of the support bar has a groove for mounting the liner plate and the middle liner plate, and the inner surface of the support bar away from its groove is integrally formed with ribs.

[0012] By adopting the above technical solution, the lining plate and the middle lining plate can be clamped at the fixed groove of the support bar, so that one side of the lining plate and the middle lining plate can be pressed against the surface of the support bar, and the support bar is reinforced with rib corners.

[0013] Furthermore, a bottom support is centrally protruding from the inner wall of the collection tube, and the bottom block of the ultrafiltration inner tube is supported at the top of the bottom support.

[0014] By adopting the above technical solution, the bottom support of the collection tube can support the bottom block of the ultrafiltration inner tube.

[0015] Furthermore, the diameter of the annular hole of the lifting ring is larger than the width of the bottom block of the ultrafiltration inner tube;

[0016] By adopting the above technical solution, the bottom block of the ultrafiltration inner tube can pass through the lifting ring, so that the filter membrane below the ultrafiltration inner tube can be supported by the middle liner plate and the counter liner plate.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] This invention features a membrane liner mechanism installed inside the collection tube of an ultrafiltration centrifuge tube. The liner mechanism's support strips and ribs are installed inside the collection tube to support and clamp the liner plate and the middle liner plate. The bottom block of the ultrafiltration inner tube can pass through the lifting ring, allowing the lower filter membrane to be supported by the middle liner plate and the liner plate. During the exosome extraction centrifugation process, the liner plate and the middle liner plate, along with the liner claw strips, support the ultrafiltration membrane of the ultrafiltration inner tube, effectively limiting the degree of deformation of the ultrafiltration membrane, preventing rupture due to excessive membrane deformation, improving the success rate of exosome extraction, and ensuring the quality of exosomes.

[0019] Furthermore, the liner plate and the middle liner plate used for supporting the filter membrane below the ultrafiltration inner tube are connected by a lifting ring. When the ultrafiltration inner tube is removed for cleaning, the lifting ring can be used to remove and clean the liner plate and the middle liner plate, making it convenient for the membrane support structure to be cleaned and maintained. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of an exosome extraction device according to the present invention.

[0021] Figure 2 This is a schematic diagram showing the disassembled components of the collection tube, ultrafiltration inner tube, and tube cap of an exosome extraction device according to this utility model.

[0022] Figure 3 This is a cross-sectional view of the collection tube of an exosome extraction device according to this utility model.

[0023] Figure 4 This is an exploded view of the membrane liner mechanism of an exosome extraction device according to this utility model.

[0024] Figure 5 This is an enlarged view of the liner claw strip of an exosome extraction device according to this utility model.

[0025] In the diagram: 1. Collection tube; 2. Ultrafiltration inner tube; 3. Tube cover; 4. Membrane liner mechanism; 5. Supporting ribs; 6. Supporting ribs; 7. Supporting corner; 8. Liner plate; 9. Middle liner plate; 10. Lifting ring; 11. Liner claw strip; 12. Bottom support. Detailed Implementation

[0026] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0027] like Figure 1-5As shown, an exosome extraction device includes a collection tube 1, an ultrafiltration inner tube 2, and a tube cap 3. The ultrafiltration inner tube 2 is fitted inside the collection tube 1, and the tube cap 3 is installed at the top of the collection tube 1. It also includes a membrane lining mechanism 4, which includes support ribs 5, ribs 6, a liner plate 8, a middle liner plate 9, a lifting ring 10, and liner claws 11. The collection tube 1 below the ultrafiltration inner tube 2 has symmetrically protruding support ribs 5, and the surface of the support ribs 5 is supported by the liner plate 8 and the middle liner plate 9 for lining the filter membrane below the ultrafiltration inner tube 2. The top of the support ribs 5 has a rib 6 protruding, and the liner plate 8 and the middle liner plate 9 are fitted between the ribs 6. The top of the liner plate 8 and the middle liner plate 9 is integrally formed with a lifting ring 10, and the liner plate 8 and the middle liner plate 9 have symmetrically protruding liner claws 11 facing the inclined surface of the ultrafiltration inner tube 2.

[0028] The strip of the liner claw 11 is integrally formed on the outside of the plate of the liner plate 8 and the middle liner plate 9, and the strip of the liner claw 11 has branch bars protruding from the outside of the strip.

[0029] By adopting the above technical solution, the filter membrane below the ultrafiltration inner tube 2 can be supported by the strip disc and branch bar of the liner plate 8 and the middle liner plate 9 with the help of the strip disc and branch bar of the liner claw strip 11.

[0030] The four sets of plates of the liner plate 8 are integrally formed below the ring of the lifting ring 10, and a middle liner plate 9 is fixed below the lifting ring 10 between every two sets of connected plates of the liner plate 8.

[0031] By adopting the above technical solution, the four sets of plates of the liner plate 8 below the lifting ring 10, together with the two sets of plates of the middle liner plate 9, can provide support for the filter membrane below the ultrafiltration inner tube 2.

[0032] The top of the support bar 6 is provided with a groove for mounting the liner plate 8 and the middle liner plate 9, and the inner surface of the support bar 6 away from its groove is integrally formed with a rib corner 7.

[0033] By adopting the above technical solution, the lining plate 8 and the middle lining plate 9 can be clamped at the fixed groove of the support bar 6, so that one side of the lining plate 8 and the middle lining plate 9 can be pressed against the surface of the support bar 5, and the support bar 6 is provided with a rib corner 7 for reinforcement.

[0034] Among them, a bottom support 12 protrudes from the center of the inner wall of the collection tube 1, and the bottom block of the ultrafiltration inner tube 2 is supported at the top of the bottom support 12.

[0035] By adopting the above technical solution, the bottom block of the ultrafiltration inner tube 2 can be supported by the bottom support column 12 of the collection tube 1.

[0036] Wherein, the diameter of the annular hole of the lifting ring 10 is larger than the width of the bottom block of the ultrafiltration inner tube 2;

[0037] By adopting the above technical solution, the bottom block of the ultrafiltration inner tube 2 can pass through the lifting ring 10, so that the filter membrane below the ultrafiltration inner tube 2 can be supported by the middle liner plate 9 and the counter liner plate 8.

[0038] It should be noted that this utility model is an exosome extraction device. It improves upon the ultrafiltration centrifuge tube used for exosome extraction by installing a membrane liner mechanism 4 inside the collection tube 1 of the ultrafiltration centrifuge tube. The supporting ribs 5 and 6 of the membrane liner mechanism 4 can be installed inside the collection tube 1. Subsequently, the middle liner plate 9 with liner claw strips 11 and the opposing liner plate 8 can be integrally formed at the lifting ring 10. The lifting ring 10 can then be inserted downwards into the tube from the opening of the collection tube 1, allowing the opposing liner plate 8 and the middle liner plate 9 to be positioned within the supporting ribs. The groove of strip 6 is secured, and one side of the liner plate 8 and the middle liner plate 9 can be pressed against the surface of the support strip 5. The support strip 6 is reinforced with rib corners 7, which can stably support the liner plate 8 and the middle liner plate 9. Subsequently, when the ultrafiltration inner tube 2 is inserted into the collection tube 1, the bottom block of the ultrafiltration inner tube 2 can pass through the lifting ring 10, so that the filter membrane below the ultrafiltration inner tube 2 can be supported by the middle liner plate 9 and the liner plate 8. After the sample containing exosomes is transferred into the ultrafiltration inner tube 2, the tube cap 3 can be installed. The collection tube 1 is then inserted into the centrifuge tube placement position. The centrifuge is started, and the liquid in the sample passes through the ultrafiltration membrane of the ultrafiltration inner tube 2 under centrifugation. The exosomes are retained in the ultrafiltration inner tube 2 above the ultrafiltration membrane. During centrifugation, the liner plate 8 and the middle liner plate 9 with the liner claw strip 11 support the ultrafiltration membrane of the ultrafiltration inner tube 2, thereby limiting the deformation of the ultrafiltration membrane during centrifugation and maintaining a relatively stable shape under centrifugal force. This helps maintain the integrity of the ultrafiltration membrane, reduces the possibility of rupture due to excessive membrane deformation, and thus improves the success rate and quality of exosome extraction. After ultrafiltration, the tube cap 3 can be opened, and the exosomes filtered in the ultrafiltration inner tube 2 can be removed and collected using a pipette. Then, the ultrafiltration inner tube 2 can be pulled out and the extraction ring 10 can be poured out. The ultrafiltration inner tube 2, collection tube 1, tube cap 3, and extraction ring 10 can be gently rinsed using PBS buffer or other suitable cleaning solution, following the existing cleaning procedure for centrifugal ultrafiltration tubes.

[0039] It should be noted that this utility model is an exosome extraction device. All components in this utility model are known to those skilled in the art, and their structure and principle can be learned by those skilled in the art through technical manuals or conventional experimental methods.

[0040] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. An exosome extraction device, comprising a collection tube (1), an ultrafiltration inner tube (2), and a cap (3), wherein the ultrafiltration inner tube (2) is fitted inside the collection tube (1), and a cap (3) is installed at the top of the collection tube (1), characterized in that: It also includes a membrane liner mechanism (4), which includes a support rib (5), a rib (6), a liner plate (8), a middle liner plate (9), a lifting ring (10), and a liner claw (11). The collection tube (1) below the ultrafiltration inner tube (2) has symmetrically protruding support ribs (5), and the surface of the support ribs (5) is supported by the liner plate (8) and the middle liner plate (9) for lining the filter membrane below the ultrafiltration inner tube (2). The top of the support ribs (5) has a rib (6), and the liner plate (8) and the middle liner plate (9) are clamped between the ribs (6). The top of the liner plate (8) and the middle liner plate (9) are integrally formed with a lifting ring (10), and the liner plate (8) and the middle liner plate (9) have symmetrically protruding liner claws (11) facing the inclined plate surface of the ultrafiltration inner tube (2).

2. The exosome extraction device according to claim 1, characterized in that: The strip of the liner claw (11) is integrally formed on the outside of the plate of the liner plate (8) and the middle liner plate (9), and the strip of the liner claw (11) has branch bars protruding from the outside of the strip.

3. The exosome extraction device according to claim 1, characterized in that: The four sets of plates of the liner plate (8) are integrally formed below the ring of the lifting ring (10), and a middle liner plate (9) is fixed below the lifting ring (10) between every two sets of connected plates of the liner plate (8).

4. The exosome extraction device according to claim 1, characterized in that: The top of the support bar (6) is provided with a fixed groove for mounting the liner plate (8) and the middle liner plate (9), and the inner surface of the support bar (6) away from its fixed groove is integrally formed with a rib corner (7).

5. The exosome extraction device according to claim 1, characterized in that: The inner wall of the collection tube (1) has a centrally protruding bottom support (12), and the bottom block of the ultrafiltration inner tube (2) is supported at the top of the bottom support (12).

6. The exosome extraction device according to claim 1, characterized in that: The diameter of the annular hole of the lifting ring (10) is greater than the width of the bottom block of the ultrafiltration inner tube (2).

Images