A stem cell perfusion device

Abstract

This utility model discloses a stem cell perfusion device, relating to the field of stem cell culture technology. The device includes a housing, with a nutrient tank fixedly connected to the top of the housing near its center. A third water pipe is fixedly connected to the top of the housing near the nutrient tank. A placement cavity is formed inside the housing near one side center. An outlet is fixedly connected to the bottom of the third water pipe, located inside the placement cavity. A support plate is fixedly connected to the bottom center of the placement cavity. A rotating shaft is rotatably connected to the top center of the support plate, and a three-pronged plate is fixedly connected to the top of the rotating shaft. This utility model solves the problem of low efficiency in perfusing stem cell nutrient solution, which is typically done manually by sequentially perfusing multiple culture flasks. The device utilizes a water pump and motor, and through the coordinated operation of the first, second, and third water pipes, the solenoid valve, the rotating shaft, and the three-pronged plate.

Description

Technical Field

[0001] This utility model relates to the field of stem cell culture technology, specifically a stem cell perfusion device. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated daughter cells. The definition of stem cells has been continuously revised over the years and defined from different perspectives. Currently, most biologists and medical professionals believe that stem cells are a type of cell derived from embryos, fetuses, or adults that possess the ability to self-renew, proliferate, and differentiate without limitation under certain conditions. They can produce daughter cells with the same phenotype and genotype as themselves, as well as specialized cells that make up body tissues and organs, and can also differentiate into progenitor cells.

[0003] Stem cell tissue fluid requires nutrient solution for cultivation during testing and other medical applications. A batch of stem cell test tubes requires a fixed amount of nutrient solution for cultivation. However, in existing technologies, the perfusion of stem cell nutrient solution is generally done manually by sequentially perfusing multiple culture flasks, which results in low work efficiency. To address the above problems, the inventors have proposed a stem cell perfusion device to solve these issues. Utility Model Content

[0004] To address the issue that the perfusion of stem cell nutrient solution typically involves manually perfusing multiple culture flasks sequentially, resulting in low work efficiency, the purpose of this invention is to provide a stem cell perfusion device.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a stem cell perfusion device, comprising a box body, a nutrient box fixedly connected to the top of the box body near the center, a water pipe three fixedly connected to the top of the box body near the nutrient box, a placement cavity opened inside the box body near the center of one side, a liquid outlet fixedly connected to the bottom end of the water pipe three inside the placement cavity, a support plate fixedly connected to the bottom center of the placement cavity, a rotating shaft rotatably connected to the top center of the support plate, a three-pronged plate fixedly connected to the top of the rotating shaft, three culture bottles evenly distributed inside the three-pronged plate near the center, and a cover plate slidably connected to the top of the three-pronged plate near the culture bottles, the three cover plates being evenly distributed.

[0006] Preferably, three electric cylinders are fixedly connected to the top of the three-pronged plate near the center, and the three electric cylinders are evenly distributed. The output end of the electric cylinder is fixedly connected to the cover plate.

[0007] Preferably, a motor is fixedly connected to the center of the bottom end of the support plate, the output end of the motor passes through the support plate and is fixedly connected to the rotating shaft, and a solenoid valve is fixedly connected to the top three ends of the water pipe.

[0008] Preferably, a liquid inlet is fixedly connected to the top of the nutrient tank and near the center of one side, and a water pump is installed inside the nutrient tank, with a water pipe fixedly connected to the side of the water pump.

[0009] Preferably, a second water pipe is fixedly connected to the other end of the water pump, and the other end of the second water pipe is fixedly connected to a solenoid valve. A controller is fixedly connected to the front end of the housing and near one side.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0011] 1. In this utility model, by setting a cover plate and an electric cylinder, the fixedly connected cover plate can be moved horizontally, at which point the nutrient solution can be poured in, thereby ensuring the sealing of the culture bottle during the pouring process.

[0012] 2. In this utility model, by operating a water pump and a motor, and through the cooperation of water pipe one, water pipe two, water pipe three, solenoid valve, rotating shaft, and three-pronged plate, the problem of low work efficiency caused by manually perfusing multiple culture bottles sequentially to perfuse stem cell nutrient solution is solved. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0015] Figure 2 This is a schematic diagram of the three-dimensional structure of the water pipe of this utility model.

[0016] Figure 3 This is a schematic diagram of the nutrient box structure of this utility model.

[0017] Figure 4 This utility model Figure 2 Enlarged structural diagram at point A in the middle.

[0018] In the diagram: 1. Box body; 11. Controller; 12. Placement chamber; 13. Liquid inlet; 2. Nutrient tank; 21. Water pump; 22. Water pipe one; 23. Water pipe two; 24. Solenoid valve; 25. Water pipe three; 26. Liquid outlet; 3. Support plate; 31. Motor; 32. Rotating shaft; 33. Tripod plate; 34. Culture bottle; 35. Cover plate; 36. Electric cylinder. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example: Figure 1-4 As shown, this utility model provides a technical solution: a stem cell perfusion device, including a box 1, a nutrient box 2 fixedly connected to the top of the box 1 near the center, a water pipe 25 fixedly connected to the top of the box 1 near the nutrient box 2, a placement cavity 12 opened inside the box 1 near the center of one side, an outlet 26 fixedly connected to the bottom of the water pipe 25 inside the placement cavity 12, a support plate 3 fixedly connected to the bottom center of the placement cavity 12, a rotating shaft 32 rotatably connected to the top center of the support plate 3, a three-pronged plate 33 fixedly connected to the top of the rotating shaft 32, three culture bottles 34 evenly distributed inside the three-pronged plate 33 near the center, and a cover plate 35 slidably connected to the top of the three-pronged plate 33 near the culture bottles 34, with the three cover plates 35 evenly distributed, and one of the culture bottles 34 being at the same level as the outlet 26.

[0021] Three electric cylinders 36 are fixedly connected to the top of the three-pronged plate 33 near the center, and the three electric cylinders 36 are evenly distributed. The output end of the electric cylinders 36 is fixedly connected to the cover plate 35.

[0022] By adopting the above technical solution, the electric cylinder 36 is operated, thereby causing the fixedly connected cover plate 35 to move horizontally.

[0023] A motor 31 is fixedly connected at the center of the bottom of the support plate 3, and the output end of the motor 31 passes through the support plate 3 and is fixedly connected to the rotating shaft 32.

[0024] By adopting the above technical solution, the motor 31 is operated, thereby causing the fixedly connected rotating shaft 32 to rotate.

[0025] A solenoid valve 24 is fixedly connected to the top of water pipe 325.

[0026] By adopting the above technical solution, a solenoid valve 24 is installed at the top of water pipe 3 25 in order to control the flow rate of the liquid.

[0027] The nutrient tank 2 has a liquid inlet 13 fixedly connected to the top and near the center of one side.

[0028] By adopting the above technical solution, an inlet 13 is provided at the top of the nutrient tank 2 to facilitate the delivery of nutrient solution into the nutrient tank 2.

[0029] The nutrient box 2 is equipped with a water pump 21, and a water pipe 22 is fixedly connected to the side of the water pump 21.

[0030] By adopting the above technical solution, the water pump 21 is operated so that the nutrient solution is transported to the inside of the water pipe 23 through the water pipe 22.

[0031] The other end of the water pump 21 is fixedly connected to a water pipe 23, and the other end of the water pipe 23 is fixedly connected to a solenoid valve 24.

[0032] By adopting the above technical solution, water pipe 23 is set up to transport the nutrient solution from water pipe 1 22 to water pipe 3 25.

[0033] A controller 11 is fixedly connected to the front end of the housing 1 and near one side.

[0034] By adopting the above technical solution, a controller 11 is set at the front end of the housing 1 to facilitate the control of the device's operation.

[0035] Working principle: When using this device, nutrient solution is first added into the nutrient tank 2 through the inlet 13. Then, the culture bottle 34 is placed inside the tri-plate 33. By setting three cover plates 35 near the center of the tri-plate 33, the culture bottle 34 is sealed. When the culture bottle 34 needs to be filled with nutrient solution, the fixed cover plates 35 are moved horizontally by operating the electric cylinder 36. At this time, the filling of nutrient solution can begin, thus ensuring the sealing of the culture bottle 34 during filling.

[0036] When perfusing multiple culture flasks 34, the water pump 21 is run to transport the nutrient solution inside the nutrient tank 2 to the water pipe 23 via the water pipe 22. Then, the solenoid valve 24 is opened to allow the nutrient solution to flow into the culture flask 34 through the outlet 26. After one flask has been perfused, the motor 31 is run to rotate the fixedly connected shaft 32, which in turn causes the triangular plate 33 to rotate the culture flask 34 in a circular motion. When the next culture flask 34 rotates to the position below the outlet 26, the perfusing process can begin. This solves the problem that the perfusing of stem cell nutrient solution is usually done manually by perfusing multiple culture flasks 34 sequentially, which results in low work efficiency.

[0037] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A stem cell perfusion device, comprising a housing (1), characterized in that: A nutrient tank (2) is fixedly connected to the top of the box (1) near the center. A water pipe (25) is fixedly connected to the top of the box (1) near the nutrient tank (2). A placement cavity (12) is opened inside the box (1) near the center of one side. An outlet (26) is fixedly connected to the bottom of the water pipe (25) inside the placement cavity (12). A support plate (3) is fixedly connected to the center of the bottom of the placement cavity (12). A rotating shaft (32) is rotatably connected to the center of the top of the support plate (3). A three-pronged plate (33) is fixedly connected to the top of the rotating shaft (32). Three culture bottles (34) are provided inside the three-pronged plate (33) near the center. The three culture bottles (34) are evenly distributed. A cover plate (35) is slidably connected to the top of the three-pronged plate (33) near the culture bottles (34). The three cover plates (35) are evenly distributed.

2. The stem cell perfusion device as described in claim 1, characterized in that, Three electric cylinders (36) are fixedly connected to the top of the three-pronged plate (33) near the center, and the three electric cylinders (36) are evenly distributed. The output end of the electric cylinders (36) is fixedly connected to the cover plate (35).

3. The stem cell perfusion device as described in claim 1, characterized in that, A motor (31) is fixedly connected at the center of the bottom end of the support plate (3), and the output end of the motor (31) passes through the support plate (3) and is fixedly connected to the rotating shaft (32).

4. The stem cell perfusion device as described in claim 1, characterized in that, A solenoid valve (24) is fixedly connected to the top of the water pipe (25).

5. The stem cell perfusion device as described in claim 1, characterized in that, The nutrient tank (2) has a liquid inlet (13) fixedly connected to the top and near the center of one side.

6. The stem cell perfusion device as described in claim 1, characterized in that, The nutrient box (2) is equipped with a water pump (21), and a water pipe (22) is fixedly connected to the side of the water pump (21).

7. The stem cell perfusion device as described in claim 6, characterized in that, The other end of the water pump (21) is fixedly connected to a water pipe (23), and the other end of the water pipe (23) is fixedly connected to a solenoid valve (24).

8. The stem cell perfusion device as described in claim 1, characterized in that, A controller (11) is fixedly connected to the front end and near one side of the housing (1).

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