A cryogenic grinding device

Abstract

The utility model discloses a kind of frozen grinding devices, belong to grinding equipment technical field.The utility model solves the problem of easy to introduce impurities, more liquid nitrogen consumption in the prior art using open grinding bowl grinding exists.The utility model includes grinding stick, bowl body and bowl cover, the through-hole for grinding stick is passed through and is arranged on the bowl cover;The grinding stick includes grinding head and grinding rod, the through slot is arranged on the grinding rod, the through slot extends to the connecting place of grinding rod and grinding head, and the area of the grinding rod in bowl body is provided with discharge hole, and the discharge hole is communicated with the through slot.The utility model is provided with the through slot on the grinding stick, liquid nitrogen can be injected in the through slot, liquid nitrogen in the through slot is gradually discharged into bowl body from discharge hole in the process of grinding, that is, liquid nitrogen is gradually added, opening bowl cover to add liquid nitrogen is avoided, it is simple to operate, not easy to introduce impurities and ensure grinding effect, effectively improve grinding efficiency.

Description

Technical Field

[0001] This utility model belongs to the technical field of grinding equipment, and specifically relates to a cryogenic grinding device. Background Technology

[0002] Liquid ammonia grinding is a crucial step in the RNA extraction process. Its main purpose is to rapidly break down cells at low temperatures, inhibiting RNase activity and ensuring the integrity and purity of the RNA, which is key to subsequent experiments. RNA molecules are fragile and easily degraded. The instantaneous low temperature of liquid nitrogen quickly cools the sample tissue, effectively inhibiting the degradation of RNA by endogenous RNases in the tissue. The ultra-low temperature properties of liquid ammonia make the sample tissue and cells extremely brittle, allowing for effective cell disruption and the release of intracellular nucleic acids through grinding.

[0003] In existing technologies, when performing liquid nitrogen grinding, the sample needs to be placed in a mortar, and liquid nitrogen is added in batches for thorough grinding. After grinding, the sample needs to be mixed with lysis buffer and transferred to centrifuge tubes for temporary storage. To facilitate the addition of liquid nitrogen, an open mortar is generally used in this process. However, using an open mortar may allow impurities to enter, affecting the purity of the sample and consequently impacting detection accuracy. Furthermore, open mortars cause faster dissipation of cold energy, resulting in higher liquid nitrogen consumption. Utility Model Content

[0004] To address the issues that existing technologies using open grinding bowls may allow impurities to enter, affecting sample purity and thus reducing detection accuracy, and that open grinding bowls cause faster dissipation of cold energy, resulting in higher liquid nitrogen consumption, this invention provides a cryogenic grinding device.

[0005] The technical solution adopted in this utility model is as follows:

[0006] A cryogenic grinding apparatus includes a grinding rod, a bowl body, and a bowl cover, wherein the bowl cover is provided with a through hole for the grinding rod to pass through;

[0007] The grinding rod includes a grinding head and a grinding rod. The grinding rod is provided with a through groove that extends to the connection between the grinding rod and the grinding head. The grinding rod is provided with a discharge hole in the area inside the bowl, and the discharge hole communicates with the through groove.

[0008] By adopting this technical solution, this invention firstly, by setting a bowl lid, can block the opening of the bowl during grinding, thus slowing down the evaporation rate of liquid nitrogen and reducing the probability of operator injury from liquid nitrogen splashing during grinding. Secondly, this invention has a through groove on the grinding rod, into which liquid nitrogen can be injected. During grinding, the liquid nitrogen in the through groove is gradually discharged into the bowl through the discharge hole, which achieves gradual addition of liquid nitrogen and avoids opening the bowl to add liquid nitrogen. This simplifies operation, ensures grinding effect, and effectively improves grinding efficiency.

[0009] Preferably, the grinding head is a heat-conducting metal grinding head, and the grinding head has a cavity that communicates with the through groove. The discharge hole is located at the junction of the grinding head and the grinding rod.

[0010] To further improve the grinding effect after adopting this technical solution, the preferred method in this invention is to set the grinding head as a heat-conducting metal grinding head, and to set a cavity in the heat-conducting metal grinding head that is connected to the through groove. When liquid nitrogen is injected into the through groove, the liquid nitrogen will first fill the cavity, and then as the liquid level increases, the liquid level will reach the discharge hole and then be discharged from the discharge hole. With this setting, the heat-conducting metal grinding head can also transfer cold energy to the workpiece to be ground during grinding, further ensuring the grinding effect.

[0011] Preferably, when the bowl lid, bowl body, and grinding rod are assembled in use, the distance from the end face of the grinding rod located outside the bowl body to the through hole is 12-15cm.

[0012] By adopting this technical solution, the height of the grinding rod is set to be longer, so that it can hold more liquid nitrogen, which can minimize the number of times liquid nitrogen needs to be added to the grinding rod. At the same time, with the increased height of the grinding rod, the operator can hold the side of the grinding rod for grinding, avoiding contact with the top surface of the grinding rod, which can prevent liquid nitrogen from injuring the operator, and also makes it easier to add liquid nitrogen.

[0013] Preferably, when the bowl lid, bowl body, and grinding rod are assembled in use, a holding member is provided on the part of the grinding rod outside the bowl body. The holding member is detachably connected to the grinding rod, and the height of the holding member is lower than the height of the groove opening.

[0014] After adopting this technical solution, a handle can be set on the grinding rod to facilitate grinding. Grinding by holding the handle can reduce the probability of hands getting cold during grinding compared to grinding by holding the grinding rod by hand.

[0015] Preferably, a cover is detachably connected to the opening of the through groove on the grinding rod.

[0016] By adopting this technical solution, the cover can seal the opening of the through-slot, thus slowing down the evaporation of liquid nitrogen.

[0017] Preferably, the diameter of the through hole is 1-3 cm larger than the diameter of the grinding rod.

[0018] By adopting this technical solution, the diameter of the through hole is larger than the diameter of the grinding rod, which ensures that the grinding rod has enough room to move for grinding.

[0019] Preferably, a plurality of discharge holes are provided, which are evenly distributed in a ring on the grinding rod, and the width of each discharge hole is 0.2-0.6 cm.

[0020] With this technical solution, the several discharge holes are evenly distributed in a ring on the grinding rod, which allows liquid nitrogen in the channel to be injected into the bowl from all directions, so that the liquid nitrogen can fully contact the material to be ground; controlling the width of the discharge holes to 0.1-0.5cm achieves the effect of slowly injecting liquid nitrogen into the bowl.

[0021] Preferably, the bottom of the bowl is provided with a heavy base.

[0022] By adopting this technical solution, grinding stability can be improved by setting a heavy base at the bottom of the bowl.

[0023] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0024] 1. This utility model firstly provides a bowl cover, which can block the opening of the bowl during grinding, thereby slowing down the evaporation of liquid nitrogen and reducing the probability of liquid nitrogen splashing and causing injury to the operator during grinding.

[0025] 2. This utility model has a through groove on the grinding rod, into which liquid nitrogen can be injected. During the grinding process, the liquid nitrogen in the through groove is gradually discharged from the discharge hole into the bowl, which realizes the gradual addition of liquid nitrogen and avoids opening the bowl to add liquid nitrogen. The operation is simple and the grinding effect is guaranteed, effectively improving the grinding efficiency. Attached Figure Description

[0026] This utility model will be described by way of example and with reference to the accompanying drawings, wherein:

[0027] Figure 1 This is a schematic diagram of the external structure of this utility model;

[0028] Figure 2 This is a schematic diagram of the internal structure of this utility model;

[0029] Figure 3 This is a schematic diagram of the connection structure between the bowl lid and the grinding rod in this utility model;

[0030] Among them: 1-bowl body, 2-bowl lid, 3-grinding rod, 4-through groove, 5-handling piece, 6-heavy base, 7-grinding head, 8-discharge hole, 9-through hole, 10-cavity. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0032] In the description of the embodiments of this application, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. In addition, the terms "first," "second," and "third," etc., are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0033] The following is combined with Figures 1-3 This utility model will be described in detail.

[0034] like Figure 1As shown, a cryogenic grinding apparatus includes a grinding rod, a bowl body 1, and a bowl lid 2. The bowl lid 2 has a through hole 9 for the grinding rod to pass through. In this embodiment, taking plant RNA extraction as an example, to avoid cross-contamination, the grinding rod, bowl body 1, and bowl lid 2 are all made of stainless steel that can withstand high-temperature sterilization. Stainless steel can also withstand the low temperatures of liquid nitrogen. During grinding, the bowl body 1 and bowl lid 2 need to be held and pressed down. Therefore, in one embodiment, a removable heat-insulating sleeve can be provided on the holding position of the grinding rod, the outer surface of the bowl body 1, and the bowl lid 2. During use, the heat-insulating sleeve is placed on the bowl body 1 and bowl lid 2 (one heat-insulating sleeve is provided on each of the grinding rod, bowl body 1, and bowl lid 2; the material of the heat-insulating sleeve can refer to oven mitts). After use, the sleeve is removed, and then the grinding rod, bowl body 1, and bowl lid 2 are removed for cleaning and high-temperature sterilization. It should be noted that to ensure the grinding effect, protrusions or grooves to increase the contact surface can be provided on the bowl body 1 and the grinding rod to improve the grinding effect. In this embodiment, the depth of the bowl 1 is approximately 10cm, and the height of the grinding rod is 25cm.

[0035] The grinding rod includes a grinding head 7 and a grinding rod 3. The grinding rod 3 has a through groove 4 extending to the connection point between the grinding rod 3 and the grinding head 7. A discharge hole 8 is provided on the grinding rod 3 within the bowl 1, and the discharge hole 8 communicates with the through groove 4. In this embodiment, the diameter of the grinding rod 3 is approximately 2 cm, and the diameter of the through groove 4 is 1.8 cm. Figure 3 As shown, the grinding head 7 is mushroom-shaped, and its arc-shaped surface fits with the inner surface of the bowl 1 to grind the material to be ground.

[0036] Taking tobacco leaves as an example, the method of using this utility model is as follows:

[0037] like Figure 1-3 As shown, the sterilized bowl 1 is placed on the operating table, and then the tobacco leaves are placed inside the bowl 1. The grinding rod 3 is then passed through the bowl cover 2 and the bowl cover 2 is closed on the bowl 1. The grinding rod 3 is then held and liquid ammonia is injected into the through groove 4 on the grinding rod 3. Liquid nitrogen enters the bowl 1 through the through groove 4 and the discharge hole 8 and comes into contact with the tobacco leaves. After the liquid nitrogen injection is completed, the operator holds the side of the grinding rod 3 and operates the grinding rod 3 so that the grinding head 7 can fully crush and grind the tobacco leaves until the grinding is finished.

[0038] In one embodiment, the grinding head 7 has a cavity 10 that communicates with the through groove 4, and the discharge hole 8 is located at the junction of the grinding head 7 and the grinding rod 3. Specifically, as shown... Figure 2As shown, the shape of cavity 10 is consistent with the shape of grinding head 7. When liquid nitrogen is injected into through groove 4, it will first fill cavity 10 located below through groove 4. Then, as liquid nitrogen continues to be injected, the liquid level continues to rise until the liquid level exceeds the position of discharge hole 8. This setting can keep the temperature of grinding head very low, which can effectively improve the grinding effect.

[0039] In one embodiment, when the bowl cover 2, bowl body 1, and grinding rod are assembled for use, a holding member 5 is provided on the part of the grinding rod 3 outside the bowl body 1. The holding member 5 is detachably connected to the grinding rod 3, and the height of the holding member 5 is lower than the height of the groove opening of the through groove 4. Specifically, an external thread can be provided on the part of the grinding rod 3 outside the bowl body 1, and an internal threaded hole that mates with the external thread can be provided on the holding member 5. The detachable connection is achieved through the engagement of the internal and external threads. It should be noted that a stop is provided at the lower end of the external thread. When the holding member 5 is rotated to the stop, it cannot be rotated further. When the operator needs to rotate it during grinding, they only need to apply force in the direction of the thread, which will not affect the normal grinding process. After grinding, the holding member 5 can be disassembled by rotating it in the opposite direction of the thread. In this embodiment, the holding member 5 is a disc surrounding the grinding rod 3. In other embodiments, it can also be other shapes, such as a ring in the middle for threaded connection with the grinding rod 3, with two holding rods symmetrically arranged on both sides of the ring for gripping.

[0040] In one embodiment, a cover is detachably connected to the opening of the through groove 4 on the grinding rod 3. The cover can be made of stainless steel to meet the requirements of high-temperature sterilization. The detachable connection can be a threaded connection. By setting the cover, the opening of the through groove 4 can be closed to slow down the evaporation of liquid nitrogen.

[0041] In one embodiment, the diameter of the through hole 9 is 1-3 cm larger than the diameter of the grinding rod 3. In this embodiment, the diameter of the through hole 9 is 2 cm larger than the diameter of the grinding rod 3, so that the grinding rod has enough room to move and can easily grind the workpiece.

[0042] In one embodiment, several discharge holes 8 are provided, which are evenly distributed in a ring on the grinding rod 3. The width of each discharge hole 8 is 0.1-0.5 cm. Specifically, in this embodiment, four discharge holes 8 are provided, which are evenly distributed at equal intervals on the grinding rod 3 and located on the same horizontal plane. Their specific width is 0.2 cm, which allows the liquid nitrogen in the channel 4 to be slowly and evenly injected into the bowl 1.

[0043] In one embodiment, a heavy base 6 is provided at the bottom of the bowl 1. Providing a heavy base 6 at the bottom of the bowl 1 improves grinding stability. Specifically, it can be made of high-density stainless steel. In other embodiments, an anti-slip pad or similar material can be provided on the bottom surface of the heavy base 6 to increase friction and further enhance stability.

[0044] The embodiments described above merely illustrate specific implementation methods of this application, and while the descriptions are detailed and specific, they should not be construed as limiting the scope of protection of this application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the technical solution of this application, and these modifications and improvements all fall within the scope of protection of this application.

Claims

1. A cryogenic grinding apparatus, characterized in that: It includes a grinding rod, a bowl (1) and a bowl cover (2), wherein the bowl cover (2) is provided with a through hole (9) for the grinding rod to pass through. The grinding rod includes a grinding head (7) and a grinding rod (3). The grinding rod (3) is provided with a through groove (4), which extends to the connection between the grinding rod (3) and the grinding head (7). The grinding rod (3) is provided with a discharge hole (8) in the area inside the bowl (1), which is connected to the through groove (4).

2. The cryogenic grinding apparatus according to claim 1, characterized in that: The grinding head (7) is a heat-conducting metal grinding head, and a cavity (10) is provided inside the grinding head (7). The cavity (10) is connected to the through groove (4), and the discharge hole (8) is provided at the junction of the grinding head (7) and the grinding rod (3).

3. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: When the bowl lid (2), bowl body (1) and grinding rod are assembled in the use state, the distance from the end face of the grinding rod (3) located outside the bowl body (1) to the through hole (9) is 12-15cm.

4. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: When the bowl cover (2), bowl body (1) and grinding rod are assembled in the use state, the part of the grinding rod (3) located outside the bowl body (1) is provided with a holding part (5). The holding part (5) is detachably connected to the grinding rod (3), and the height of the holding part (5) is lower than the height of the groove opening of the through groove (4).

5. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: A cover can be detachably connected to the opening of the through groove (4).

6. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: The diameter of the through hole (9) is 1-3 cm larger than the diameter of the grinding rod (3).

7. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: The discharge holes (8) are provided in a plurality of manner, and the plurality of discharge holes (8) are evenly distributed in a ring on the grinding rod (3), and the width of each discharge hole (8) is 0.1-0.5cm.

8. A cryogenic grinding apparatus according to claim 1 or 2, characterized in that: The bottom of the bowl (1) is provided with a heavy base (6).

Images