A device for inoculating against contact contamination
The inoculation device, which uses a combination of ultraviolet lamps and electric heating for sterilization, solves the problem of contamination associated with traditional inoculation devices, achieving highly efficient aseptic inoculation operations. It is suitable for microbial fermentation and cell culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANDONG XINGGUANG CAPITAL BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-08-28
- Publication Date
- 2026-07-28
AI Technical Summary
Traditional inoculation devices pose a risk of contamination during the disinfection process, and their unreasonable structural design makes it easy for the inoculated material to come into contact with the outside air, making it impossible to effectively avoid contamination by other microorganisms.
The sterilization process employs a combination of ultraviolet lamps and electric heating, along with a detachable butterfly valve and outer shell design, to ensure sterility during inoculation.
It effectively kills miscellaneous bacteria, reduces the risk of contamination during inoculation, and is simple and reliable to operate, suitable for microbial fermentation and cell culture.
Smart Images

Figure CN224564586U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microbial inoculation equipment technology, specifically to an inoculation device that prevents contact contamination. Background Technology
[0002] In biotechnology fields such as microbial fermentation and cell culture, inoculation is a crucial step in transferring cultured microorganisms or cells to new culture media. Ensuring a sterile inoculation environment is paramount during this process. Contamination of the seed tank or fermenter by other microorganisms can range from affecting product yield and quality and increasing production costs to potentially causing complete fermentation or culture failure and resulting in significant economic losses.
[0003] Traditional inoculation devices and methods have many drawbacks. For example, some devices rely solely on simple flame sterilization, which is susceptible to contamination due to factors such as air stability, human error, and the inherent dangers of flame sterilization. Other devices use steam sterilization, but the residual moisture in the steam creates a humid environment that promotes bacterial growth, further increasing the risk of contamination. In addition, some inoculation devices have flawed structural designs that allow the inoculated material to come into contact with the outside air during operation, making it impossible to effectively prevent contamination by other microorganisms. Utility Model Content
[0004] In view of the deficiencies in the prior art, this utility model provides an inoculation device that prevents contact contamination.
[0005] This utility model is achieved through the following technical solution: An inoculation device for preventing contact contamination includes an inoculation bottle with a butterfly valve connected to its outlet end. The other end of the butterfly valve is connected to a housing. The housing has an inlet end that mates with the butterfly valve. The housing contains an ultraviolet (UV) lamp sterilization section and an electric heating section. The UV lamp sterilization section is located at the end of the housing near the butterfly valve, and the electric heating section is located at the end of the housing away from the butterfly valve. The UV lamp sterilization section includes a glass tube and a UV lamp. The glass tube is connected to the inlet end of the housing, and the UV lamp is located within the cavity between the glass tube and the housing. The electric heating section includes a heating wire and a dispensing tube. The dispensing tube is connected to the glass tube, and the heating wire is located within the cavity between the dispensing tube and the housing.
[0006] Preferably, a chuck is provided at both the outlet end of the inoculation bottle and the inlet end of the outer shell, and both ends of the butterfly valve are locked onto the chuck by clamps.
[0007] Preferably, the outer shell is a rotating structure.
[0008] Preferably, the ultraviolet lamp is ring-shaped and is installed in the cavity between the glass tube and the outer shell via a lamp holder.
[0009] Preferably, the number of ultraviolet lamps is greater than 1, and they are arranged in the cavity between the glass tube and the outer shell.
[0010] Preferably, the heating wire is spiral-shaped and is wrapped around the cavity between the liquid outlet pipe and the outer shell.
[0011] The beneficial effects of this utility model are as follows: This utility model can effectively kill miscellaneous bacteria and reduce the risk of contamination during the inoculation process through the dual sterilization effect of the heating jacket and the ultraviolet lamp device; the detachable design of the butterfly valve and the outer shell facilitates maintenance; the overall device of this utility model is easy to operate and highly reliable, and is suitable for inoculation operations in the fields of microbial fermentation and cell culture. Attached Figure Description
[0012] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.
[0013] Figure 1 This is the front view of the present invention.
[0014] Figure 2 This is a structural view of the present invention, omitting the chuck and clamp.
[0015] Figure 3 This is a structural view of the present invention, omitting the chuck and clamp.
[0016] Figure 4 This is a structural view of the chuck and clamp of this utility model.
[0017] In the attached diagram: 1. Inoculation bottle; 101. Butterfly valve; 2. Outer shell; 3. Ultraviolet lamp sterilization part; 4. Electric heating part; 301. Lamp holder; 302. Ultraviolet lamp; 303. Glass tube; 401. Heating wire; 501. Chuck; 502. Clamp. Detailed Implementation
[0018] To make the above-mentioned objects, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below.
[0019] It should be noted that when a component is said to be "fixed to" another component, it can be directly attached to the other component or there may be an intervening component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there may be an intervening component.
[0020] For ease of explanation, spatial relative terms such as “up,” “down,” “left,” and “right” may be used herein to describe the relationship of one element or feature shown in the figure relative to another element or feature. It should be understood that, in addition to the orientation shown in the figure, spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the figure is inverted, an element described as being “down” of other elements or features would be positioned “up” of those other elements or features. Therefore, the exemplary term “down” can encompass both up and down orientations.
[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.
[0022] The present invention will now be described in detail with reference to the accompanying drawings: An inoculation device for preventing contact contamination includes an inoculation bottle 1, with a butterfly valve 101 connected to the outlet end of the inoculation bottle 1. The other end of the butterfly valve 101 is connected to a housing 2, which is a rotating structure made of stainless steel. The housing 2 has an inlet end that cooperates with the butterfly valve 101. An ultraviolet lamp sterilization section 3 and an electric heating section 4 are disposed inside the housing 2.
[0023] The ultraviolet lamp sterilization section 3 is used to sterilize the butterfly plate outlet side of the butterfly valve 101 and the inside of the glass tube 303. It utilizes the bactericidal effect of ultraviolet light to destroy the DNA structure of microorganisms, thereby achieving disinfection. The electric heating section 4 is used to sterilize the outlet tube, killing any bacteria that may be present on the surface of the outlet tube through heating, ensuring that the inoculation pipeline is in a sterile state.
[0024] The butterfly valve 101 includes a valve body, a butterfly plate, and a driving device. The butterfly plate is rotatably disposed in the valve body, and the driving device is connected to the butterfly plate to drive the butterfly plate to rotate, thereby controlling the opening and closing state of the inoculation bottle 1.
[0025] The ultraviolet lamp sterilization section 3 is located inside the outer shell 2 at one end near the butterfly valve 101, and the electric heating section 4 is located inside the outer shell 2 at the end away from the butterfly valve 101. The ultraviolet lamp sterilization section 3 includes a glass tube 303 and an ultraviolet lamp 302. The glass tube 303 is made of high-strength transparent glass and is connected to the inlet end of the outer shell 2. The ultraviolet lamp 302 is located in the cavity between the glass tube 303 and the outer shell 2. The electric heating section 4 includes a heating wire 401 and a liquid outlet pipe. The liquid outlet pipe is connected to the glass tube 303, and the heating wire 401 is located in the cavity between the liquid outlet pipe and the outer shell 2.
[0026] Both the outlet end of the inoculation bottle 1 and the inlet end of the outer shell 2 are equipped with chucks 501, and both ends of the butterfly valve 101 are locked to the chucks 501 by clamps 502.
[0027] The ultraviolet lamp 302 is ring-shaped and is mounted in the cavity between the glass tube 303 and the outer shell 2 via a lamp holder 301. The number of ultraviolet lamps 302 is greater than one, and they are arranged in a specific configuration within the cavity between the glass tube 303 and the outer shell 2. The ultraviolet lamps 302 are powered by an external power supply.
[0028] The heating wire 401 is spiral-shaped and wraps around the cavity between the liquid outlet tube and the outer shell 2. The heating wire 401 is powered by an external power source, and the heating temperature and time can be precisely controlled to meet different sterilization requirements.
[0029] The ultraviolet lamp 302 and heating wire 401 are powered by an external power source, which avoids the occurrence of insufficient power supply from the storage battery during the sterilization process.
[0030] The inoculation bottle 1 is made of high-pressure resistant and corrosion-resistant stainless steel to ensure the stability and safety of the inoculation bottle 1 during sterilization and inoculation.
[0031] Operating procedures: 1. Before inoculation: Activate the heating mantle to sterilize the pipe above the inoculation port valve (i.e., the outlet pipe) at high temperature (e.g., maintain 100℃ for 30 seconds); at the same time, turn on the ultraviolet lamp 302 to disinfect the outside of the butterfly plate of the butterfly valve 101 and the inside of the glass tube 303 by irradiation (e.g., ultraviolet wavelength 254nm, irradiation for 2 minutes).
[0032] 2. During inoculation: Turn off the ultraviolet lamp 302 and heating wire 401, open the butterfly valve 101 and the inoculation port valve to complete the inoculation operation.
[0033] 3. After inoculation: Reactivate heating wire 401 and ultraviolet lamp 302 to disinfect the parts that have been in contact with the virus a second time to prevent the growth of residual bacteria.
[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.
Claims
1. An inoculation device for preventing contact contamination, comprising an inoculation bottle (1), characterized in that: The outlet end of the inoculation bottle (1) is connected to a butterfly valve (101), and the other end of the butterfly valve (101) is connected to a shell (2). The shell (2) is provided with an inlet end that cooperates with the butterfly valve (101). The shell (2) is provided with an ultraviolet lamp sterilization part (3) and an electric heating part (4). The ultraviolet lamp sterilization part (3) is located at one end of the shell (2) near the butterfly valve (101), and the electric heating part (4) is located at one end of the shell (2) away from the butterfly valve (101). The ultraviolet lamp sterilization part (3) includes a glass tube (303) and an ultraviolet lamp (302). The glass tube (303) is connected to the inlet end of the shell (2), and the ultraviolet lamp (302) is located in the cavity between the glass tube (303) and the shell (2). The electric heating part (4) includes a heating wire (401) and an outlet tube. The outlet tube is connected to the glass tube (303), and the heating wire (401) is located in the cavity between the outlet tube and the shell (2).
2. The inoculation device for preventing contact contamination as described in claim 1, characterized in that: Both the outlet end of the inoculation bottle (1) and the inlet end of the outer shell (2) are equipped with chucks (501), and both ends of the butterfly valve (101) are locked onto the chucks (501) by clamps (502).
3. The inoculation device for preventing contact contamination as described in claim 1, characterized in that: The outer shell (2) is a rotating structure.
4. The inoculation device for preventing contact contamination as described in claim 3, characterized in that: The ultraviolet lamp (302) is ring-shaped and is installed in the cavity between the glass tube (303) and the outer shell (2) through the lamp holder (301).
5. The inoculation device for preventing contact contamination as described in claim 4, characterized in that: The number of ultraviolet lamps (302) is greater than 1, and they are arranged in the cavity between the glass tube (303) and the outer shell (2).
6. The inoculation device for preventing contact contamination as described in claim 3, characterized in that: The heating wire (401) is spiral-shaped and is wrapped around the cavity between the liquid outlet pipe and the outer shell (2).