A continuous flow oligonucleotide synthesis reactor

Abstract

The utility model discloses a continuous flow oligonucleotide synthesis reactor belongs to reactor technical field, this continuous flow oligonucleotide synthesis reactor includes bottom plate, fixed mounting is saved in the bottom plate's water storage tank, fixed mounting is saved in the water storage tank's steel sheet, sets up the reaction unit and sets up the unloading mechanism for installing and disassembling reaction unit for the steel sheet one side at steel sheet top, through the cooperation of above -mentioned each device, through the setting of unloading mechanism, the convex block of reaction unit both sides is aligned with the sliding block, makes the convex block first contact the inclined plane of sliding block, then continues to press down, along with fixed link and pushes the sliding block to both sides, after reaction unit installation in place, two sliding blocks will slide in fixed link, and hold the convex block, realize fixed, when disassembling, push the sliding rod, make control lever slide in the fixed frame, drive two inclined blocks to move to both sides, and then make the sliding block synchronous sliding, loosen the convex block, thereby improve the dismounting efficiency of device.

Description

Technical Field

[0001] This invention relates to the field of reactor technology, specifically a continuous flow oligonucleotide synthesis reactor. Background Technology

[0002] Oligonucleotides are generally defined as linear polynucleotide fragments consisting of 2 to 10 nucleotide residues linked by phosphodiester bonds. However, there is no strict limitation on the specific number of nucleotide residues when using this term. In many literatures, polynucleotide molecules containing 30 or more nucleotide residues are also classified as oligonucleotides. Oligonucleotides can be synthesized automatically by instruments and are widely used in modern molecular biology research, such as as primers and probes for DNA synthesis.

[0003] Upon investigation, a utility model patent discloses a microchannel continuous flow reactor for drug synthesis (publication number: CN218307881U), comprising a steel frame, several reaction units, an inlet channel and an outlet channel. The inlet channel and outlet channel are arranged side by side at the bottom of the steel frame, and the several reaction units are arranged side by side at intervals on the inlet channel and outlet channel. Each reaction unit includes a fixed frame, a reaction plate and a heat exchange plate. The reaction plate is made of glass and has multiple intersecting vortex channels inside. The heat exchange plate is attached to both sides of the reaction plate. The fixed frame wraps around the edges of the reaction plate and the heat exchange plate. The fixed frame is detachably connected to the steel frame by bolts. The bottom of the fixed frame is provided with an inlet quick connector and an outlet quick connector. The inlet quick connector connects the inlet channel to the inlet of the heat exchange plate, and the outlet quick connector connects the outlet channel to the outlet of the heat exchange plate.

[0004] In the aforementioned patent, by bridging the inlet and outlet water channels, the heat exchange plates built into several reaction units are arranged in parallel, and the temperature, hydraulic pressure, and flow rate of the heat exchange fluid flowing inside are kept consistent, and the heat removed by the heat exchange plates is the same, ensuring the safety of the reaction fluid reaction inside the reaction plates. However, during the use of this device, multiple reaction units need to be fixed to the steel frame one by one with bolts. Therefore, the subsequent disassembly and installation of the reaction units require a lot of manpower for bolting operations, thereby reducing the disassembly and assembly efficiency of the device.

[0005] Therefore, this invention provides a continuous flow oligonucleotide synthesis reactor to solve the above problems. Utility Model Content

[0006] This invention provides a continuous flow oligonucleotide synthesis reactor, which aims to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a continuous flow oligonucleotide synthesis reactor, the continuous flow oligonucleotide synthesis reactor comprising a bottom plate, a water tank fixedly installed on the bottom plate, a steel plate fixedly installed on the water tank, a reaction unit disposed above the steel plate, and a loading and unloading mechanism disposed on one side of the steel plate for installing and disassembling the reaction unit.

[0008] The loading and unloading mechanism includes a fixed frame fixedly installed on one side of the steel plate, a fixed rod fixedly installed between the inner walls of both sides of the fixed frame, a slider slidably installed on the side surface of the fixed rod, an inclined block fixedly installed at the bottom of the slider, a sliding rod slidably installed inside the fixed frame, and a control rod fixedly installed at the top of the sliding rod.

[0009] As a preferred technical solution of this application, multiple reaction units are provided, and the reaction units are arranged in a linear array on the steel plate. Multiple fixing frames are provided, and the fixing frames are arranged in a linear array on one side of the steel plate. The sliding rod passes through the fixing frame, and the control rod is slidably connected inside the two inclined blocks.

[0010] As a preferred technical solution of this application, a spring is sleeved on the side surface of the fixing rod, one end of the spring is fixedly connected to one side of the slider, the other end of the spring is fixedly connected to the inner wall of one side of the fixing frame, and a push rod is fixedly connected to the bottom of the plurality of sliders.

[0011] As a preferred technical solution of this application, a water pump is fixedly connected to the top of the base plate, the pump's pumping end is fixedly connected to the water storage tank, the pump's outlet end is fixedly connected to a first flexible hose, and a water channel is fixedly connected to the end of the first flexible hose away from the pump.

[0012] As a preferred technical solution of this application, the top of the water channel is fixedly connected to the bottom of the plurality of reaction units, the side of the water tank away from the water pump is fixedly connected to a heat dissipation fin, and the end of the water channel away from the first hose is fixedly connected to a second hose.

[0013] As a preferred technical solution of this application, the second hose is S-shaped and extends through the plurality of heat dissipation fins, and the end of the second hose away from the water channel is fixedly connected to the side of the water tank away from the water pump.

[0014] The beneficial effects of this utility model are as follows: This utility model has a simple structure and is easy to use. By setting up a loading and unloading mechanism, the protrusions on both sides of the reaction unit are aligned with the slider, so that the protrusions first contact the inclined surface of the slider, and then continue to press down, pushing the slider to both sides along the fixed rod. After the reaction unit is installed in place, the two sliders will slide in the fixed rod, locking the protrusions and achieving fixation. When disassembling, push the sliding rod to make the control rod slide in the fixed frame, driving the two inclined blocks to move to both sides, thereby making the sliders slide synchronously and releasing the protrusions, thus improving the disassembly and assembly efficiency of the device. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the structure of the present invention. Figure 2 ; Figure 3 This is a cross-sectional view of the fixing frame in this utility model; Figure 4 for Figure 3 Enlarged view of point A in the middle; Figure 5 This is a schematic diagram of the installation of the slider in this utility model.

[0016] In the picture: 1. Base plate; 2. Water tank; 3. Steel plate; 4. Reaction unit; 5. Fixing frame; 6. Fixing rod; 7. Sliding block; 8. Inclined block; 9. Spring; 10. Sliding rod; 11. Control rod; 12. Push rod; 13. Water pump; 14. First hose; 15. Water channel; 16. Heat dissipation fins; 17. Second hose. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] This invention provides a continuous flow oligonucleotide synthesis reactor, such as... Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, the continuous flow oligonucleotide synthesis reactor includes a bottom plate 1, a water tank 2 fixedly installed on the bottom plate 1, a steel plate 3 fixedly installed on the water tank 2, a reaction unit 4 disposed above the steel plate 3, and a loading and unloading mechanism disposed on one side of the steel plate 3 for installing and disassembling the reaction unit 4.

[0019] The loading and unloading mechanism includes a fixed frame 5 fixedly installed on one side of the steel plate 3, a fixed rod 6 fixedly installed between the inner walls on both sides of the fixed frame 5, a slider 7 slidably installed on the side surface of the fixed rod 6, an inclined block 8 fixedly installed at the bottom of the slider 7, a sliding rod 10 slidably installed inside the fixed frame 5, and a control rod 11 fixedly installed at the top of the sliding rod 10.

[0020] Multiple reaction units 4 are provided, and the reaction units 4 are arranged in a linear array on the steel plate 3. Multiple fixing frames 5 are provided, and the fixing frames 5 are arranged in a linear array on one side of the steel plate 3. The slide rod 10 passes through the fixing frame 5, and the control rod 11 is slidably connected inside the two inclined blocks 8.

[0021] When using this continuous flow oligonucleotide synthesis reactor, the base plate 1 is first placed in a suitable position, and the protrusions on both sides of the reaction unit 4 are aligned with the slider 7. The protrusions on both sides of the reaction unit 4 first contact the inclined surface of the slider 7. Continue to press down and push the slider 7 to both sides along the fixing rod 6. After the reaction unit 4 is installed in the appropriate position, the two corresponding sliders 7 slide inward along the fixing rod 6, thereby locking the protrusions on both sides of the reaction unit 4, thus completing the fixation of the reaction unit 4. When it is necessary to disassemble the reaction unit 4, the sliding rod 10 can be pushed to make the control rod 11 slide within the fixing frame 5. The sliding of the control rod 11 causes the two inclined blocks 8 to slide to both sides. The sliding of the inclined blocks 8 drives the slider 7 fixedly connected to them to move synchronously, thereby releasing the protrusions on both sides of the reaction unit 4, which facilitates the installation and disassembly of the reaction unit 4 and improves the efficiency of installation and disassembly.

[0022] Furthermore, in order to enable each component to automatically reset, such as Figure 1 , Figure 4 and Figure 5 As shown, a spring 9 is sleeved on the side surface of the fixed rod 6. One end of the spring 9 is fixedly connected to one side of the slider 7, and the other end of the spring 9 is fixedly connected to the inner wall of one side of the fixed frame 5. A push rod 12 is fixedly connected to the bottom of multiple sliders 10.

[0023] In use, the continuous flow oligonucleotide synthesis reactor is equipped with spring 9, which allows slider 7 to automatically reset when no external force is applied, ensuring that reaction unit 4 can be stably fixed. When slider 7 is pushed to slide to both sides to release reaction unit 4, spring 9 is compressed and stores energy. Once the external force disappears, spring 9 releases the stored energy, pushing slider 7 to slide inward, thus achieving the automatic reset function. The push rod 12 allows users to operate multiple sliders 10 simultaneously, improving the efficiency of disassembling or installing reaction unit 4. Users only need to push push rod 12 to control the sliding of all sliders 10 at the same time, thereby controlling the movement of all inclined blocks 8 and slider 7.

[0024] It should be noted that, in order to reduce the possibility of heat-carrying fluids re-entering the interior of reaction unit 4, such as... Figure 1 , Figure 2 and Figure 3 As shown, a water pump 13 is fixedly connected to the top of the base plate 1. The pumping end of the water pump 13 is fixedly connected to the water storage tank 2. The outlet end of the water pump 13 is fixedly connected to a first hose 14. A water channel 15 is fixedly connected to the end of the first hose 14 away from the water pump 13.

[0025] The top of the water channel 15 is fixedly connected to the bottom of multiple reaction units 4. A heat dissipation fin 16 is fixedly connected to the side of the water tank 2 away from the water pump 13. A second hose 17 is fixedly connected to the end of the water channel 15 away from the first hose 14.

[0026] The second hose 17 is S-shaped and passes through multiple heat dissipation fins 16. The end of the second hose 17 away from the water channel 15 is fixedly connected to the side of the water tank 2 away from the water pump 13.

[0027] In operation, the continuous flow oligonucleotide synthesis reactor uses a water pump 13 to extract fluid from the water tank 2 and transport it through a first hose 14 to a water channel 15. The water channel 15 evenly distributes the fluid to the bottom of each reaction unit 4, cooling the reaction unit 4. The cooled water then flows into a second hose 17. Since the second hose 17 is S-shaped and has multiple heat dissipation fins 16, the fluid makes full contact with the heat dissipation fins 16 during its flow, transferring the heat it carries to the heat dissipation fins 16 to achieve a heat dissipation effect. Finally, the cooled fluid flows back into the water tank 2, forming a circulating cooling system. This effectively reduces the amount of heat-carrying fluid re-entering the reaction unit 4, ensuring the stability of the internal temperature of the reaction unit 4, thereby improving the efficiency and quality of the synthesis reaction.

[0028] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A continuous flow oligonucleotide synthesis reactor, characterized in that: The continuous flow oligonucleotide synthesis reactor includes a base plate (1), a water tank (2) fixedly installed on the base plate (1), a steel plate (3) fixedly installed on the water tank (2), a reaction unit (4) disposed above the steel plate (3), and a loading and unloading mechanism disposed on one side of the steel plate (3) for installing and disassembling the reaction unit (4). The loading and unloading mechanism includes a fixed frame (5) fixedly installed on one side of the steel plate (3), a fixed rod (6) fixedly installed between the inner walls on both sides of the fixed frame (5), a slider (7) slidably installed on the side surface of the fixed rod (6), an inclined block (8) fixedly installed at the bottom of the slider (7), a sliding rod (10) slidably installed inside the fixed frame (5), and a control rod (11) fixedly installed on the top of the sliding rod (10).

2. The continuous flow oligonucleotide synthesis reactor according to claim 1, characterized in that: Multiple reaction units (4) are provided, and the reaction units (4) are arranged in a linear array on the steel plate (3). Multiple fixing frames (5) are provided, and the fixing frames (5) are arranged in a linear array on one side of the steel plate (3). The sliding rod (10) passes through the fixing frame (5), and the control rod (11) is slidably connected inside the two inclined blocks (8).

3. The continuous flow oligonucleotide synthesis reactor according to claim 1, characterized in that: A spring (9) is sleeved on the side surface of the fixed rod (6). One end of the spring (9) is fixedly connected to one side of the slider (7), and the other end of the spring (9) is fixedly connected to the inner wall of one side of the fixed frame (5). A push rod (12) is fixedly connected to the bottom of the plurality of slide rods (10).

4. The continuous flow oligonucleotide synthesis reactor according to claim 1, characterized in that: A water pump (13) is fixedly connected to the top of the base plate (1). The pumping end of the water pump (13) is fixedly connected to the water storage tank (2). The outlet end of the water pump (13) is fixedly connected to a first hose (14). A water channel (15) is fixedly connected to the end of the first hose (14) away from the water pump (13).

5. A continuous flow oligonucleotide synthesis reactor according to claim 4, characterized in that: The top of the water channel (15) is fixedly connected to the bottom of the plurality of reaction units (4), the water tank (2) is fixedly connected to a heat dissipation fin (16) on the side away from the water pump (13), and the end of the water channel (15) away from the first hose (14) is fixedly connected to a second hose (17).

6. The continuous flow oligonucleotide synthesis reactor according to claim 5, characterized in that: The second hose (17) is S-shaped and passes through the plurality of heat dissipation fins (16). The end of the second hose (17) away from the water channel (15) is fixedly connected to the side of the water tank (2) away from the water pump (13).

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