An antibody affinity screening separation device

Abstract

The utility model discloses an antibody affinity screening separation device, including centrifuge body, the surface of centrifuge body is provided with centrifugal storehouse, the inner chamber of centrifugal storehouse is provided with drive link, the top outer circle surface fixed connection of drive link has a plurality of groups of connecting piece, and the fixed connection of the fixed sheet is established between two connecting pieces of every group, and the fixed sheet's outside top fixed connection has the first fixed plate, and the bottom of every group fixed sheet is connected with the second fixed plate that is opposite to the first fixed plate through the connecting piece, and the fixed assembly for fixing the test tube of different diameters is arranged between the first limiting board and the second limiting board of the same side, the utility model discloses the setting of fixed assembly adjusts the coincidence area of containing hole and round hole, and this fixes the test tube of different diameters to greatly improve the applicability of device, and simple operation greatly improves the use convenience of staff.

Description

Technical Field

[0001] This utility model relates to the technical field of antibody affinity screening and separation devices, specifically an antibody affinity screening and separation device. Background Technology

[0002] Affinity screening can yield high-purity, high-specificity antibodies, which is crucial for in-depth research into fundamental biological questions such as the mechanism of antigen-antibody interactions and the structure-function relationship of proteins. During affinity screening, the target substance to be separated specifically binds to the ligands immobilized on the affinity medium, while other impurities do not bind or bind weakly. Centrifugation can rapidly separate the affinity medium from the solution, removing unbound impurities and retaining the target substance bound to the affinity medium, thereby achieving preliminary separation and enrichment.

[0003] Currently, Chinese patent CN220091725U discloses an antibody separation and screening device, including a centrifuge chamber, a support, a test tube holder, and a clamp. A central rotating shaft is rotatably connected to the center of the centrifuge chamber, the support is fixedly connected to the central rotating shaft, and the test tube holder is rotatably connected to the support. This device employs a horizontal rotor centrifuge design, allowing for convenient and safe placement of test tubes by simply inserting them vertically into the test tube slot. During centrifugation, the centrifugation angle of the test tubes can be fixed by clamping the slide plate with the clamp when the test tubes are at the desired angle.

[0004] The antibody separation and screening device described above still has some problems in use. When using test tubes of different diameters in experiments, the device may not be able to be fixed well, or it may be necessary to frequently change different test tube holders or other parts, which makes the operation inconvenient. Utility Model Content

[0005] The purpose of this invention is to provide an antibody affinity screening and separation device to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] An antibody affinity screening and separation device includes a centrifuge body. A centrifuge chamber is provided on the surface of the centrifuge body. A drive rod is provided in the inner cavity of the centrifuge chamber. The lower end of the drive rod is connected to the output shaft of the centrifuge body through a coupling. Several sets of connecting plates are fixedly connected to the top outer surface of the drive rod. Each set of connecting plates includes two connecting plates. A fixing plate is fixedly connected between the two connecting plates in each set. A first fixing plate is fixedly connected to the top outer side of the two fixing plates in each set. A second fixing plate is connected to the bottom of the two fixing plates in each set through a connector and is arranged opposite to the first fixing plate. The top surface of the first fixing plate has receiving holes for accommodating test tubes on both the front and rear sides. A fixing assembly for fixing test tubes of different diameters is provided between the first fixing plate and the second fixing plate on the same side.

[0008] The fixing assembly includes a first limiting plate and a second limiting plate disposed on the same side between the first fixing plate and the second fixing plate. The surfaces of the first limiting plate and the second limiting plate are provided with circular holes corresponding to each set of receiving holes. The top of the first limiting plate is connected to the bottom of the first fixing plate through a sliding connector. A fixing block is fixedly connected to the bottom outer side of the first limiting plate. The bottom of the fixing block is fixedly connected to the top outer side of the second limiting plate. A pushing assembly is provided in the middle outer side of the fixing plate to push the fixing block outward, thereby driving the first limiting plate and the second limiting plate to move outward and fix the test tube.

[0009] As a preferred technical solution, the pushing component includes a second wedge block fixedly connected to the middle of the inner side of the fixed block, the inclined surface of the second wedge block contacts the first wedge block, and the interior of the fixed block is provided with a pushing member that pushes the first wedge block upward to push the second wedge block outward;

[0010] The pushing component includes a circular groove formed inside the fixed plate and arranged along its height. A lead screw is rotatably connected to the inner cavity of the circular groove. A nut seat is threadedly connected to the surface of the lead screw located inside the circular groove. The side of the circular groove facing the first wedge block is open. One end of the nut seat passes through the opening of the circular groove and is fixedly connected to the end of the first wedge block. The end of the lead screw rotates upward and passes through the circular groove. A hexagonal groove is formed at the end of the lead screw.

[0011] As a preferred technical solution, the connector includes a connecting plate that is slidably inserted into the bottom of each set of fixing plates, the bottom of each set of connecting plates is fixedly connected to the top of the corresponding side of the second fixing plate, and the surface of each set of connecting plates is provided with an anti-detachment component to prevent the connecting plate from detaching.

[0012] As a preferred technical solution, the anti-detachment component includes a limiting hole opened on the lower inner surface of each set of fixing plates, a connecting bolt fixedly connected to the inner surface of each set of connecting plates, each set of connecting bolts passing through the corresponding side limiting hole and threadedly connected to a connecting nut, and one side of each set of connecting nuts abutting against the surface of the corresponding side fixing plate.

[0013] As a preferred technical solution, the sliding connector includes a limiting block fixedly installed on the top of each group of first limiting plates. Each group of first fixed plates has a limiting groove at its bottom, corresponding to the position of the limiting block, for the limiting block to slide. The limiting groove is provided along the length of the first fixed plate. Each group of limiting blocks is slidably connected to the inner cavity of the limiting groove on the corresponding side. Each group of first fixed plates has a fixing member at its bottom for fixing the limiting block. Each group of first fixed plates has a resetting member on its outer side of the limiting groove, which pushes the limiting block inward to reset the corresponding side of the first limiting plate.

[0014] As a preferred technical solution, the cross-section of each set of limiting blocks is arranged in a T-shape.

[0015] As a preferred technical solution, the fastener includes a connecting hole opened on the top of each group of first fixing plates and communicating with the limiting groove. The inner cavity of each connecting hole is fitted with a fixing bolt. The end of each group of fixing bolts passes through the connecting hole and is threaded to the top surface of the limiting block. The two sides of the ends of each group of fixing bolts respectively contact the top surface of the corresponding side of the first fixing plate.

[0016] As a preferred technical solution, the reset assembly includes a circular groove formed inside each group of first fixing plates and located outside the limiting groove. A sliding disk is slidably connected inside the circular groove. A spring is fixedly connected to the outer end of the sliding disk. The other end of the spring is fixedly connected to the inner wall of the end of the corresponding side circular groove. A connecting rod is fixedly connected to the inner side of the sliding disk. The end of the connecting rod is fixedly passed through the circular groove and fixedly connected to the end of the corresponding side limiting block.

[0017] As a preferred technical solution, the inner wall of each set of circular holes is provided with an anti-slip layer.

[0018] As a preferred technical solution, a groove is provided on the top outer side of the first limiting plate, and the inner cavity of the groove is provided with scale lines.

[0019] Compared with the prior art, the beneficial effects of this utility model are:

[0020] 1. This utility model, through the setting of the fixing component and the threaded transmission of the lead screw and nut seat, drives the first wedge block to move upward. At the same time, the upward movement of the first wedge block pushes the second wedge block to move outward, which in turn drives the first limiting plate and the second limiting plate to move outward simultaneously through the fixing block, thereby adjusting the overlapping area of ​​the receiving hole and the round hole, thus fixing test tubes of different diameters, thereby greatly improving the applicability of the device, and the operation is simple, greatly improving the convenience of use for the staff.

[0021] 2. By using a connector, this utility model allows operators to adjust the height of the second fixing plate by sliding the connecting plate when dealing with test tubes of different heights. This satisfies the need to fix taller test tubes and greatly expands the applicability of this centrifuge technology to test tubes of different sizes in antibody affinity screening and separation experiments.

[0022] 3. By setting up a reset component, when the test tube needs to be removed, the elastic potential energy of the spring is converted into the kinetic energy of the sliding disk and the connecting rod, which drives the limiting block and the first limiting plate to move inward and return to the initial position. This not only improves work efficiency but also reduces the complexity of manual operation and enhances the convenience of antibody affinity screening and separation experiments. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the antibody affinity screening and separation device of this utility model;

[0024] Figure 2 This is a schematic diagram of the structure of the fixing piece of this utility model;

[0025] Figure 3 This is a cross-sectional view of the fixing piece of this utility model;

[0026] Figure 4 This is a cross-sectional view of the first fixing plate of this utility model;

[0027] Figure 5 This is a schematic diagram of the top structure of the first limiting plate of this utility model.

[0028] In the picture:

[0029] 100. Centrifuge body; 101. Centrifuge chamber; 102. Drive rod; 103. Connecting plate;

[0030] 200. Fixing plate; 202. Second fixing plate; 203. First fixing plate; 204. First limiting plate; 205. First wedge block; 206. Second wedge block; 207. Second limiting plate; 208. Receiving groove; 209. Receiving hole; 210. Fixing block; 211. Round hole;

[0031] 300. Connecting nut; 301. Connecting bolt; 302. Limiting hole; 303. Connecting plate;

[0032] 400. Nut seat; 401. Lead screw; 402. Circular groove; 403. Hexagonal groove;

[0033] 500. Fixing bolt; 501. Connecting hole; 502. Limiting groove; 503. Connecting rod; 504. Limiting block; 505. Circular groove; 506. Spring; 507. Sliding disc;

[0034] 600, Groove; 601, Scale line; 602, Anti-slip layer. Detailed Implementation

[0035] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0036] Please see Figure 1-5 This embodiment provides an antibody affinity screening and separation device, including a centrifuge body 100. A centrifuge chamber 101 is disposed on the surface of the centrifuge body 100. A drive rod 102 is disposed within the inner cavity of the centrifuge chamber 101. The lower end of the drive rod 102 is connected to the output shaft of the centrifuge body 100 via a coupling. Several sets of connecting pieces 103 are fixedly connected to the top outer surface of the drive rod 102. Each set of connecting pieces 103 includes two connecting pieces 103. A fixing piece 200 is fixedly connected between the two connecting pieces 103 in each set. The outer top of each set of two fixing pieces 200... The first fixing plate 203 is fixedly connected to the bottom of each set of two fixing plates 200. The bottom of each set of two fixing plates 200 is connected to a second fixing plate 202 that is opposite to the first fixing plate 203 via a connector. The top surface of the first fixing plate 203 has receiving holes 209 on both the front and rear sides for accommodating test tubes. A fixing component for fixing test tubes of different diameters is provided between the first fixing plate 203 and the second fixing plate 202 on the same side. The top of the second limiting plate 207 and the corresponding position of each set of receiving holes 209 have receiving grooves 208 for supporting the bottom of the test tubes.

[0037] The fixing assembly includes a first limiting plate 204 and a second limiting plate 207 disposed on the same side between a first fixing plate 203 and a second fixing plate 202. The surfaces of the first limiting plate 204 and the second limiting plate 207 are provided with circular holes 211 at positions corresponding to each set of receiving holes 209. The top of the first limiting plate 204 is connected to the bottom of the first fixing plate 203 through a sliding connector. A fixing block 210 is fixedly connected to the bottom outer side of the first limiting plate 204. The bottom of the fixing block 210 is fixedly connected to the top outer side of the second limiting plate 207. A pushing assembly is provided at the middle outer side of the fixing piece 200 to push the fixing block 210 outward, thereby driving the first limiting plate 204 and the second limiting plate 207 to move outward and fix the test tube.

[0038] The pushing component includes a second wedge block 206 fixedly connected to the middle of the inner side of the fixing block 210. The inclined surface of the second wedge block 206 contacts the first wedge block 205. The interior of the fixing block 210 is provided with a pushing member that pushes the first wedge block 205 upward to push the second wedge block 206 outward.

[0039] The pushing component includes a circular groove 402 formed inside the fixed plate 200 and arranged along its height. A lead screw 401 is rotatably connected to the inner cavity of the circular groove 402. A nut seat 400 is threadedly connected to the surface of the lead screw 401 located inside the circular groove 402. The side of the circular groove 402 facing the first wedge block 205 is open. One end of the nut seat 400 passes through the opening of the circular groove 402 and is fixedly connected to the end of the first wedge block 205. The end of the lead screw 401 rotates upward and passes through the circular groove 402. A hexagonal groove 403 is formed at the end of the lead screw 401. The screw 401 and the nut seat 400 are connected by a threaded drive, which drives the first wedge block 205 to move upward. At the same time, the upward movement of the first wedge block 205 pushes the second wedge block 206 to move outward. This, in turn, drives the first limiting plate 204 and the second limiting plate 207 to move outward simultaneously via the fixing block 210. This reduces the overlapping area of ​​the receiving hole 209 and the round hole 211, thereby fixing test tubes of different diameters. This greatly improves the applicability of the device and makes it easy to operate, thus greatly improving the convenience for the staff.

[0040] The centrifuge body 100 is a well-known technical means in the art. For its specific structure and working principle, please refer to the centrifuge body 100 in the antibody separation and screening device with publication number CN220091725U. Other details will not be elaborated here.

[0041] The connector includes a connecting plate 303 that slides into the bottom of each set of fixing plates 200. The bottom of each set of connecting plates 303 is fixedly connected to the top of the corresponding side of the second fixing plate 202. The surface of each set of connecting plates 303 is provided with an anti-detachment component to prevent the connecting plates 303 from detaching. With the connection, when dealing with test tubes of different heights, the operator can change the height of the second fixing plate 202 by sliding the connecting plate 303, thereby meeting the fixing requirements of taller test tubes and greatly expanding the applicability of this centrifuge technology to test tubes of different specifications in antibody affinity screening and separation experiments.

[0042] The anti-detachment component includes a limiting hole 302 on the lower inner surface of each set of fixing plates 200, and a connecting bolt 301 fixedly connected to the inner surface of each set of connecting plates 303. Each set of connecting bolts 301 passes through the corresponding limiting hole 302 and is threadedly connected to a connecting nut 300. One side of each set of connecting nuts 300 abuts against the surface of the corresponding side fixing plate 200. Through the setting of the anti-detachment component, the connecting bolts 301 and connecting nuts 300 effectively prevent the connecting plate 303 from detaching from the fixing plate 200 during the experiment, ensuring the structural stability of the device during operation. When the centrifuge is running at high speed and generates a large centrifugal force, this stable connection can ensure the integrity of the test tube fixing structure and avoid test tube fixing failure due to the detachment of the connecting plate 303, thereby ensuring the smooth progress of the antibody affinity screening and separation experiment.

[0043] The sliding connector includes a limiting block 504 fixedly installed on the top of each set of first limiting plates 204. Each set of first fixing plates 203 has a limiting groove 502 at its bottom, corresponding to the limiting block 504, for sliding. The limiting groove 502 is set along the length of the first fixing plate 203. Each set of limiting blocks 504 is slidably connected to the inner cavity of the corresponding side limiting groove 502. Each set of first fixing plates 203 has a fixing member at its bottom for fixing the limiting block 504. Each set of first fixing plates 203 has a resetting member on its outer side of the limiting groove 502, which pushes the limiting block 504 inward, thereby resetting the corresponding side first limiting plate 204. The cross-section of each set of limiting blocks 504 is T-shaped. Through the sliding connector, the limiting block 504 slides within the limiting groove 502, restricting the movement direction of the first limiting plate 204 and preventing the first limiting plate 204 from shifting during movement.

[0044] The fastener includes a connecting hole 501 on the top of each set of first fixing plates 203 and connected to the limiting groove 502. A fixing bolt 500 is inserted into the inner cavity of each connecting hole 501. The end of each fixing bolt 500 passes through the connecting hole 501 and is threaded to the top surface of the limiting block 504. The two sides of the end of each fixing bolt 500 contact the top surface of the corresponding side of the first fixing plate 203. By setting the fastener, the fixing bolt 500 passes through the connecting hole 501 on the top of the first fixing plate 203 and is threaded to the top of the limiting block 504, which can simply and effectively fix the limiting block 504 in the required position, thereby accurately locking the position of the first limiting plate 204, ensuring that the test tube is firmly fixed during centrifugation, and avoiding the impact of the limiting plate movement on the accuracy of the antibody affinity screening and separation experiment.

[0045] The reset assembly includes a circular groove 505 located inside each first fixed plate 203 and outside the limiting groove 502. A sliding disk 507 is slidably connected inside the circular groove 505. A spring 506 is fixedly connected to the outer end of the sliding disk 507. The other end of the spring 506 is fixedly connected to the inner wall of the end of the corresponding side circular groove 505. A connecting rod 503 is fixedly connected to the inner side of the sliding disk 507. The end of the connecting rod 503 passes through the circular groove 505 and is fixedly connected to the end of the corresponding side limiting block 504. With the reset assembly, when the test tube needs to be removed, the elastic potential energy of the spring 506 is converted into the kinetic energy of the sliding disk 507 and the connecting rod 503, which drives the limiting block 504 and the first limiting plate 204 to move inward and return to the initial position. This not only improves work efficiency but also reduces the complexity of manual operation and enhances the convenience of antibody affinity screening and separation experiments.

[0046] Each set of circular holes 211 has an anti-slip layer 602 on its inner wall. The anti-slip layer 602 significantly increases the friction between the test tube and the limiting plate, which can effectively prevent the test tube from sliding in the circular holes 211. This ensures that the test tube remains in a stable and fixed state throughout the entire antibody affinity screening and separation process, thereby improving the accuracy and reliability of the experimental results.

[0047] The anti-slip layer 602 is one or more of rubber, silicone, and polyurethane combined together.

[0048] The first limiting plate 204 has a groove 600 on its top outer side, and a scale line 601 is provided in the inner cavity of the groove 600. By setting the scale line 601, when adjusting the position of the first limiting plate 204 to adapt to different test tube diameters, the scale line 601 allows the operator to accurately control the moving distance, so that the limiting plate can fix the test tube more accurately.

[0049] Working principle;

[0050] A mixed solution containing multiple antibodies is incubated with a solidified antigen to allow the antibodies to specifically bind to the antigen and form an antigen-antibody complex. These complexes are then dispersed in an appropriate buffer and transferred to test tubes in the centrifuge chamber 101 of the centrifuge body 100.

[0051] Then, the test tube containing the sample is inserted into the receiving hole 209 on the surface of the first fixing plate 203, and pushed downwards to pass through the round holes 211 on the surfaces of the first limiting plate 204 and the second limiting plate 207 in sequence, while the end is inserted into the corresponding receiving groove 208 on the surface of the second fixing plate 202, so as to achieve the initial fixation of the test tube.

[0052] When the diameter of the test tube is smaller than the receiving hole, loosen the fixing bolt 500 and insert an Allen wrench into the hexagonal groove 403 at the top of the lead screw 401. Simultaneously rotate the lead screw 401, which in turn moves the nut seat 400 threaded onto the surface of the lead screw 401 upwards. This, in turn, moves the first wedge block 205 upwards via the nut seat 400, and the first wedge block 205 contacts the inclined surface of the second wedge block 206. The upward movement of the first wedge block 205 pushes the second wedge block 206 outwards. Fixedly connected to the fixing block 210, thereby driving the fixing block 210 to move outward. As the fixing block 210 moves outward, the first limiting plate 204 and the second limiting plate 207 move outward simultaneously, thereby reducing the overlapping area of ​​the receiving hole 209 and the round hole 211, thus fixing the test tube and preventing it from falling off. At the same time as the first limiting plate 204 moves outward, the limiting block 504 slides along the inside of the limiting groove 502, and the connecting rod 503 compresses the spring 506 in the round groove 505.

[0053] At this point, tighten the fixing bolts 500 so that the two sides of the head of the fixing bolts 500 contact the surface of the first fixing plate 203, thereby fixing the first limiting plate 204.

[0054] When the test tube is too tall, the staff uses a wrench to reach into the centrifuge chamber 101 and loosens the connecting nut 300 to release the restriction on the connecting plate 303. Then, according to the height of the test tube, the staff pushes the connecting nut 300 downward. The connecting bolt 301 connected by the thread of the connecting nut 300 moves the connecting plate 303 downward, thereby adjusting the height of the second fixing plate 202 to accommodate taller test tubes. When the plate is moved to the appropriate height, the staff tightens the connecting nut 300 to fix the connecting plate 303.

[0055] At this time, the centrifuge is started. The output shaft of the centrifuge body 100 drives the drive rod 102 to rotate through the coupling. The connecting piece 103, the fixing piece 200 and other components at the top of the drive rod 102 rotate accordingly. The test tube fixed on the fixing piece 200 also begins to make circular motion. Under the action of centrifugal force, the antigen-antibody complex will separate or precipitate in the test tube according to its size, density and other characteristics. The antigen-antibody complex with stronger affinity may precipitate to the bottom of the test tube, while unbound antibodies or other impurities may be distributed in the upper or middle layer of the solution.

[0056] When centrifugation is complete and the test tube needs to be removed, use a hex wrench to turn the screw 401 in the opposite direction, which will cause the first wedge block 205 to move downward, and the second wedge block 206 will be reset under the push of the spring 506, thus making it easier to remove the test tube.

[0057] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An antibody affinity screening separation device, characterized in that, The centrifuge includes a centrifuge body (100), on the surface of which a centrifuge chamber (101) is provided. A drive rod (102) is provided inside the centrifuge chamber (101). The lower end of the drive rod (102) is connected to the output shaft of the centrifuge body (100) via a coupling. Several sets of connecting plates (103) are fixedly connected to the top outer surface of the drive rod (102). Each set of connecting plates (103) includes two connecting plates (103), and a fixing device is fixedly connected between each set of two connecting plates (103). Each set of two fixing plates (200) has a first fixing plate (203) fixedly connected to the top of its outer side. The bottom of each set of two fixing plates (200) is connected to a second fixing plate (202) that is opposite to the first fixing plate (203) by a connector. The top surface of the first fixing plate (203) has receiving holes (209) for accommodating test tubes on both the front and rear sides. A fixing component for fixing test tubes of different diameters is provided between the first fixing plate (203) and the second fixing plate (202) on the same side. The fixing assembly includes a first limiting plate (204) and a second limiting plate (207) disposed on the same side between the first fixing plate (203) and the second fixing plate (202). The surfaces of the first limiting plate (204) and the second limiting plate (207) are provided with circular holes (211) at positions corresponding to each set of receiving holes (209). The top of the first limiting plate (204) is connected to the bottom of the first fixing plate (203) through a sliding connector. A fixing block (210) is fixedly connected to the bottom outer side of the first limiting plate (204). The bottom of the fixing block (210) is fixedly connected to the top outer side of the second limiting plate (207). The outer side of the fixing piece (200) is provided with a pushing assembly that pushes the fixing block (210) outward, thereby driving the first limiting plate (204) and the second limiting plate (207) to move outward to fix the test tube.

2. An antibody affinity screening isolation device according to claim 1, wherein: The pushing component includes a second wedge block (206) fixedly connected to the middle of the inner side of the fixed block (210), the inclined surface of the second wedge block (206) contacts the first wedge block (205), and the interior of the fixed block (210) is provided with a pushing member that pushes the first wedge block (205) upward to push the second wedge block (206) outward; The pushing component includes a circular groove (402) formed inside the fixing plate (200) and arranged along its height. A lead screw (401) is rotatably connected to the inner cavity of the circular groove (402). A nut seat (400) is threadedly connected to the surface of the lead screw (401) located inside the circular groove (402). The side of the circular groove (402) facing the first wedge block (205) is open. One end of the nut seat (400) passes through the opening of the circular groove (402) and is fixedly connected to the end of the first wedge block (205). The end of the lead screw (401) rotates upward and passes through the circular groove (402). A hexagonal groove (403) is formed at the end of the lead screw (401).

3. An antibody affinity screening isolation device according to claim 2, wherein: The connector includes a connecting plate (303) that is slidably inserted into the bottom of each set of fixing plates (200). The bottom of each set of connecting plates (303) is fixedly connected to the top of the corresponding side of the second fixing plate (202). The surface of each set of connecting plates (303) is provided with an anti-detachment component to prevent the connecting plate (303) from detaching.

4. An antibody affinity screening isolation device according to claim 3, wherein: The anti-detachment component includes a limiting hole (302) opened on the lower inner surface of each set of fixing plates (200), and a connecting bolt (301) is fixedly connected to the inner surface of each set of connecting plates (303). Each set of connecting bolts (301) passes through the corresponding side limiting hole (302) and is threadedly connected to a connecting nut (300). One side of each set of connecting nuts (300) abuts against the surface of the corresponding side fixing plate (200).

5. The antibody affinity screening isolation device of claim 1, wherein: The sliding connector includes a limiting block (504) fixedly installed on the top of each group of first limiting plates (204), and a limiting groove (502) for sliding of the limiting block (504) is provided at the bottom of each group of first fixing plates (203) and at a position corresponding to the limiting block (504). The limiting groove (502) is provided along the length of the first fixing plate (203). Each group of limiting blocks (504) is slidably connected to the inner cavity of the limiting groove (502) on the corresponding side. The bottom of each group of first fixing plates (203) is provided with a fixing member for fixing the limiting block (504). Each group of first fixing plates (203) is provided with a resetting member on the outside of the limiting groove (502) to push the limiting block (504) inward, thereby driving the corresponding side first limiting plate (204) to reset.

6. An antibody affinity screening isolation device according to claim 5, wherein: Each set of limiting blocks (504) has a T-shaped cross-section.

7. An antibody affinity screening isolation device according to claim 6, wherein: The fastener includes a connecting hole (501) opened on the top of each set of first fixing plates (203) and connected to the limiting groove (502). The inner cavity of each connecting hole (501) is fitted with a fixing bolt (500). The end of each set of fixing bolts (500) passes through the connecting hole (501) and is threaded to the top surface of the limiting block (504). The two sides of the ends of each set of fixing bolts (500) respectively contact the top surface of the corresponding side of the first fixing plate (203).

8. An antibody affinity screening isolation device according to claim 7, wherein: The reset assembly includes a circular groove (505) formed inside each group of first fixing plates (203) and located outside the limiting groove (502). A sliding disk (507) is slidably connected inside the circular groove (505). A spring (506) is fixedly connected to the outer end of the sliding disk (507). The other end of the spring (506) is fixedly connected to the inner wall of the end of the corresponding side circular groove (505). A connecting rod (503) is fixedly connected to the inner side of the sliding disk (507). The end of the connecting rod (503) is fixedly passed through the circular groove (505) and fixedly connected to the end of the corresponding side limiting block (504).

9. The antibody affinity screening isolation device of claim 1, wherein: The inner wall of each set of circular holes (211) is provided with an anti-slip layer (602).

10. An antibody affinity screening isolation device according to any one of claims 1 to 9, wherein: The first limiting plate (204) has a groove (600) on its top outer side, and the inner cavity of the groove (600) is provided with scale lines (601).

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