Pesticide residue rapid detection pretreatment extraction and sampling device

Abstract

The utility model provides pesticide residue rapid detection pretreatment extraction sampling device relates to pesticide residue detection pretreatment device field, including sample liquid tray whole tray transfer module, extraction liquid batch quantitative transfer module, sample batch oscillation extraction module, buffer solution bottle, sample batch oscillation extraction module includes sample tray frame, drive arrangement I, be equipped with 18 recesses of placing sample cup on sample tray frame, and the buffer solution is added to 3 sample cups through 3 pipetting line I, pipette pump I one to one, and sample tray frame turns 6 times and can complete 18 sample cup liquid addition, sample liquid tray whole tray transfer module includes carousel, drive arrangement II that drives carousel rotation, and the carousel is inserted with colorimetric dish, extraction liquid batch quantitative transfer module includes pipetting line II, pipetting line III and liquid adding pump II for pumping the reagent to be tested into colorimetric dish, and power device for driving the up-and-down movement of lifting platform. Advantageous effects: through this device, the extraction sampling process of batch pretreatment can be simply and quickly completed.

Description

Technical Field

[0001] This utility model relates to the technical field of pretreatment devices for pesticide residue detection, and in particular provides a rapid pesticide residue detection pretreatment extraction and sample addition device. Background Technology

[0002] With food safety receiving increasing attention, rapid detection of pesticide residues has become an important means of ensuring the safety of agricultural products.

[0003] Sample pretreatment, as a crucial step in the testing process, directly impacts testing efficiency, accuracy, and cost. With the rapid growth in testing demands, simple, fast, and batch-processable sample pretreatment methods have become a key focus for industry development.

[0004] The company previously developed a dual-rotor optical detection module reagent automatic addition device and a portable food safety detector, and applied for a patent with the application number: 2025220978400. This detector can realize the extraction, sample addition and subsequent detection process of pretreatment.

[0005] For larger-volume, faster processing, the aforementioned pre-treatment must be completed on the detector before subsequent testing can be carried out. However, if a dedicated pre-treatment device were available to provide the pre-treatment process for the detector, the portable food safety detector could directly perform the testing, which would be more efficient. Therefore, there is an urgent need for a pre-treatment device.

[0006] Furthermore, pesticide residue testing currently generally uses rotary discs, including 18-channel and 24-channel discs. If there were a dedicated pretreatment device to perform pretreatment to extract and add the sample to the cuvette, it would better meet the testing needs of more detectors.

[0007] In particular, the high-throughput enzyme-inhibited pesticide residue detection process, specifically GB / T 5009.199 "Rapid Determination of Organophosphorus and Carbamate Pesticide Residues in Vegetables" is a widely used standard method for rapid pesticide residue detection. At the grassroots level, there is an urgent need for simple and rapid batch pretreatment of samples for rapid pesticide residue detection using this method. The simple and rapid batch pretreatment is as follows: add only one buffer solution, mix it with the sample to be tested, and then transfer it (i.e., add it to a cuvette), but do not perform subsequent detection. Subsequent detection needs to be transferred to other detectors.

[0008] Therefore, we have specially developed a pretreatment extraction and sampling device for rapid detection of pesticide residues to realize the pretreatment process, which is more suitable for the pretreatment process of high-throughput enzyme inhibition pesticide residue detection. Utility Model Content

[0009] The purpose of this invention is to provide a pretreatment extraction and sampling device for rapid detection of pesticide residues that is specifically designed for pretreatment processes, reduces manual operation, increases work efficiency, and is more convenient to use.

[0010] The technical solution of this utility model is: A rapid detection device for pesticide residues includes an operating table, on which are sequentially arranged a sample liquid tray transfer module, an extract batch quantitative transfer module, a sample batch shaking extraction module, and a buffer solution batch quantitative sample addition module for adding buffer solution. The buffer batch quantitative sample addition module includes at least a buffer bottle and a pipette pump I for adding the buffer solution in the buffer bottle into the sample cup through a pipette line I, wherein the inlet of the pipette line I is inserted into the buffer bottle; Furthermore, one end of the inverted "L"-shaped placement rack is inserted into the bottom surface of the operating table and the two are fixed together with screws. Thus, the two sides of the buffer solution bottle are restricted between the operating table and the placement rack. Since the buffer solution bottle is a glass bottle, the placement rack restricts the buffer solution bottle to the left and right to avoid tipping. Furthermore, a hook-shaped groove is provided at the top of the placement rack on the right side of the operating table. The sample batch shaking extraction module includes a circular sample tray embedded in the operating table and a driving device I located inside the operating table and at the bottom of the sample tray for driving the sample tray to rotate. The sample tray has a plurality of circularly arrayed grooves, each groove serving as a hole for inserting a sample cup. Furthermore, the grooves are arranged in at least one ring radiating outward from the center point of the sample tray, with multiple grooves in each ring. The grooves are used to place the sample cups, and the sample to be tested is placed in the sample cups. The sample includes washed vegetable and / or fruit fragments, etc. a sample cups form a sample group, and there are a total of b sample groups on the sample tray, where a and b are at least 2. The buffer batch quantitative sample addition module includes a pipette pumps I. The buffer solution in the buffer bottle is added to a sample cups one-to-one through a pipette line I and a pipette pumps I. The lower ends of a outlets of a pipette line I are respectively aligned with a sample cups. Then, each time the driving device I drives the sample tray to rotate, the top of the next group of a sample cups is aligned with a outlet of a pipette line I. The buffer batch quantitative sample addition module includes a dosing rack, which is fixed on the operating table, and each of the a pipette lines I is fixed on the dosing rack; Preferably, the groove is provided in at least two concentric rings extending outward from the center point of the sample tray, with multiple grooves in each ring. Preferably, the driving device I is a stepper motor, the drive shaft of which is fixedly connected to the sample tray, thereby providing rotational power and angular positioning to the sample tray, realizing the mixing of reagents in the sample cup and accurate positioning relative to each sample group. The number of times the driving component drives the sample tray to rotate is the same as the number of sample groups, which is b. Furthermore, each of the sample cups is provided with a heating module at the bottom. The heating module includes a circular heating plate disposed at the bottom of the sample tray. The heating plate is connected to a temperature controller at its bottom for controlling the temperature of the heating plate. The temperature controller is connected to a controller, and the controller controls the temperature value of the temperature controller. Furthermore, after the driving device I drives the sample tray to rotate once and return to its original position, buffer solution is added to the sample cup of each sample group. Preferably, the number of a is 3, so that the line connecting the drug outlets of the 3 pipettes I forms an isosceles triangle; the number of b is 18, so that the drive device I can add buffer solution to all 18 sample cups by rotating 6 times. The buffer solution bottle for holding the buffer solution is located on one side of the operating table. The pipette pump I can be fixed inside the operating table or placed on the operating table. There are no restrictions on its location, as long as it can achieve liquid extraction.

[0011] The pipette pump I is a peristaltic pump I, a micro-injection pump I, or a dosing pump I, preferably the peristaltic pump I; the addition volume can be controlled by changing the pipette line I with different inner diameters; when it is a liquid addition pipette pump I or a peristaltic pump I, it also includes a suction needle, and the buffer solution in the buffer bottle is added to the sample cup in sequence through the suction needle I, pipette line I, pipette pump I, pipette line I, and injection needle II; The sample tray transfer module includes a turntable embedded in the operating table and a drive device II fixed in the operating table to drive the turntable to rotate. The turntable has ≥b grooves arranged in a ring, and cuvettes can be inserted into the grooves. Preferably, the driving device II is a motor, and the transmission shaft of the motor is connected to the turntable to drive the turntable to rotate; The batch quantitative transfer module for the extract includes *a* pipette lines II, *a* pipette lines III, and *a* dispensing pumps II for drawing the mixed, shaken, and homogenized test reagent from each set of sample cups into the cuvettes; and a power device for driving the lifting platform up and down. *a* pipette lines II and *a* pipette lines III are fixed to the lifting platform. The lower end of the inlet of *a* pipette lines II is aligned one-to-one with *a* sample cups in a sample set, and the lower end of the outlet of *a* pipette lines III is aligned one-to-one with *a* adjacent cuvettes. The *a* dispensing pumps II, through *a* pipette lines II, draw the test reagent from each of the *a* sample cups in a sample set into the *a* adjacent cuvettes in one continuous operation via *a* pipette lines III. The power unit can be a stepper motor or a mini cylinder. The lifting platform is fixed to the stepper motor or mini cylinder to realize the up and down movement of the lifting platform, further controlling whether the pipetting line II can draw the test reagent. The reason for realizing the up and down movement function is that after the liquid is drawn from one set of the sample group, directly rotating the sample tray will cause the next set of sample cups to scrape against pipetting line I. Lifting can avoid the above problem and reduce the problem of drug addition deviation caused by deformation of pipetting line I. Secondly, the descent of the lifting platform can ensure that liquid can be drawn. Thirdly, the liquid volume drawn each time is limited. For example, after drawing twice, the lifting platform rises, and further drawing will not reach the liquid surface. Therefore, the liquid volume added each time is fixed. After rising, if the liquid is not in contact with the liquid surface, air is drawn until all the liquid in the pipetting line III is drawn into the cuvette. This reduces the problem of cross-contamination caused by mixing with the next set of the sample group. Furthermore, the power unit also includes a device for driving the lifting platform to rotate. The power unit can be a combination of two motors, in which case, from top to bottom, it consists of the lifting platform, motor VII for driving the lifting platform to rotate circumferentially, motor VII at the bottom driving the motor above it, and motor VIII for moving the lifting platform up and down. Alternatively, it can be a combination of the lifting platform, a mini cylinder, and a motor, where the motor is used for circumferential rotation and the cylinder is used for moving the platform up and down. This application does not impose any limitations, as long as the above functions can be achieved. The reason for implementing the rotation function is twofold: first, it serves as a prelude when the turntable is transferred to the testing instrument (an instrument other than those in this application; this device is a prelude to the subsequent testing process), ensuring that the top of the turntable is not obstructed; second, it allows for adjustment so that the a pipettes II can be inserted into the a sample cups respectively. Furthermore, the liquid addition pump II is a peristaltic pump II, a micro-injection pump II, or a drug metering pump II, preferably the peristaltic pump II; the addition volume is controlled by changing the pipetting tubing II and III with different inner diameters and by controlling the aspiration time of the pipetting tubing II; when it is a liquid addition pump II or a peristaltic pump II, it also includes a suction needle III, and then the buffer solution in the sample cup is added to the sample cup in sequence through the suction needle III, the pipetting tubing II, the liquid addition pump II, the pipetting tubing III, and the injection needle IV; Furthermore, the aspiration needle III and the filter screen are connected by a limiting structure and an elastic element to achieve a buffer connection. The aspiration needle ensures the stability of the aspiration process, and the filter screen ensures the filtration effect of the aspiration process, thus avoiding blockage of the pipetting lines II and III. Furthermore, the liquid pump II mentioned in section a can be fixed on the lifting platform or placed on the sample tray rack, as long as it can realize liquid extraction and delivery; Preferably, the number of 'a' is 3, and the lines connecting the inlets of the pipetting line II form an isosceles triangle; the lines connecting the outlets of the pipetting line III form an arc. Furthermore, each of the lifting platform and the dosing rack is equipped with a sensor for detecting whether there is a sample cup or cuvette below; Preferably, the sensor can be an optical sensor such as a laser sensor or an infrared reflective photoelectric sensor, with an infrared reflective photoelectric sensor being preferred. The principle is that when the infrared emitter emits infrared light, it encounters the cuvette or sample cup in its path and is reflected back. This reflected infrared light is detected by the receiver. When the cuvette or sample cup is not present, the receiver receives a strong infrared signal, while when the cuvette or sample cup approaches the sensor, the received infrared signal becomes weaker.

[0012] Furthermore, this device incorporates the controller and a battery that provides power to the device. The sensor sends a signal to the controller, and the next step of adding reagents is triggered only when the sample cup or cuvette is present. In addition, the operating table is equipped with at least 5 function keys, namely: "Start / Stop", "Cleaning", "Lifting", "Turntable Movement", and "Number of Channels". All of the above function keys are electrically connected to the controller, and the drive device I, drive device II, power device, and sensor are electrically connected to the controller. Various start / stop states can be controlled through the 5 function keys. Furthermore, the turntables adopt a fully extractable structure, meaning that all the turntables and cuvettes on them can be extracted at once and transferred to subsequent detection instruments for testing. This rapid pesticide residue detection pretreatment extraction and sampling device is only used for batch addition of buffer solution and batch processing before transfer to detection instruments other than those described in this application. Specifically, this can be achieved through the following structure: First, the lifting platform adopts a slender structure. After the test solution is added to the cuvettes, during the upward extraction process of the turntables, the power device drives the lifting platform to rotate, ensuring that it does not obstruct the area directly above the turntables. Then, the drive device II is fixed inside the operating table, and the turntables are detachably connected to the drive shaft of the drive device II. The detachable connection can be achieved by providing a limiting block on the drive shaft. The bottom surface of the turntable is provided with a limiting groove that matches the shape of the limiting block. The limiting block is inserted into the limiting groove and the drive shaft is inserted into the turntable. Due to the limiting effect of the limiting block, the drive device II can drive the turntable to rotate circumferentially without affecting the lifting process. The center of the top of the turntable is provided with a threaded hole. After the screw with the nut is screwed into the threaded hole of the turntable, there is a certain gap between the bottom surface of the nut and the top surface of the turntable. When transferring to the testing instrument later, the nut can be lifted manually. In order to avoid contamination, a robotic arm can be inserted into the gap between the turntable and the nut. After locking, the robotic arm lifts upward and then the turntable and the cuvette on it are put into the matching testing instrument, thus forming a complete set of rapid pesticide residue detection lines with reduced contamination.

[0013] The advantages and positive effects of this utility model are: We have specially developed a pretreatment device for pesticide residue detection to meet the needs of subsequent detection, thereby improving overall efficiency and making it more suitable for high-throughput enzyme-inhibited pesticide residue detection pretreatment processes.

[0014] We have developed a pretreatment extraction and sample addition device for rapid detection of pesticide residues that reduces manual operation, thus avoiding the problems of forgetting to add buffer solution or adding too much / too little to a sample cup, reducing cross-contamination. It can add buffer solution to multiple sample cups at the same time, thereby improving work efficiency. After obtaining the extract, it can be added to multiple cuvettes at the same time, resulting in a more efficient and automated device.

[0015] The aspiration needle ensures the stability of the aspiration process, and the filter screen ensures the filtration effect of the aspiration process, preventing blockage of pipetting lines II and III.

[0016] When it is necessary to heat the extract in the sample cup, each sample cup is equipped with a heating module at the bottom, which enables the heating of the extract.

[0017] By installing sensors on the dosing rack and lifting platform, the buffer solution is prevented from being added to the sample cup when there is no sample cup directly below the outlet of pipette line I; and the extract is prevented from being added to the cuvette when there is no cuvette directly below the outlet of pipette line III.

[0018] This rapid pesticide residue detection pretreatment extraction and sampling device is only used to add buffer solution in batches and transfer it to a detection instrument other than those described in this application for detection. The turntable adopts an integral extraction structure, which means that all the turntables and the cuvettes on them can be extracted to the subsequent detection instrument for detection at one time, which facilitates the subsequent detection process and improves work efficiency.

[0019] The L-shaped rack prevents the buffer solution bottles from tipping over.

[0020] By adding the buffer solution in groups of three at a time and then transferring them in groups of three later, the work efficiency is higher. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the pretreatment extraction and sampling device for rapid detection of pesticide residues of this utility model with the dosing rack and lifting platform removed.

[0022] Figure 2 This is a bottom view of the pretreatment extraction and sampling device for rapid detection of pesticide residues according to this utility model.

[0023] Figure 3 This is a schematic diagram of the pretreatment extraction and sampling device for rapid detection of pesticide residues according to this utility model.

[0024] Figure 4 This is a schematic diagram of the inside of the control panel.

[0025] Figure 5 This is a schematic diagram of the lifting platform.

[0026] Figure 6 This is a schematic diagram of the connection structure between the turntable and drive device II.

[0027] Figure 7 This is a schematic diagram of a pretreatment extraction and sampling device for rapid detection of pesticide residues, equipped with liquid addition pump I and liquid addition pump II.

[0028] Figure 8 Is Figure 7 Based on this, a schematic diagram of the pretreatment extraction and sampling device for rapid detection of pesticide residues under the cover of liquid addition pump I and liquid addition pump II is presented.

[0029] In the picture: 1. Operating table; 2. Buffer bottle; 3. Pipette I; 4. Placement rack; 5. Groove; 6. Sample tray rack; 7. Drive unit I; 8. Sample cup; 9. Dosing rack; 10. Turntable; 11. Drive unit II; 12. Cuvette; 13. Pipette II; 14. Pipette III; 15. Power unit; 16. Function keys; 17. Lifting platform; 18. Limit block; 19. Limit groove; 20. Threaded hole. Detailed Implementation

[0030] For ease of understanding, Examples 1-7 of this application first describe the pretreatment steps for high-throughput enzyme-inhibited pesticide residue detection, and then describe the device structure that works in conjunction with the above pretreatment steps.

[0031] Example 1:

[0032] The automated extraction process before high-throughput enzyme-inhibited pesticide residue detection is as follows: (1) Select representative vegetable samples in batches, rinse off the surface soil, cut into square pieces about 1cm in size, take 1g of each sample and put them into sample cup 8 respectively; (2) The buffer solution in the buffer bottle 2 of the buffer batch quantitative sample addition module is added to the three sample cups 8 by the three pipette pumps I and three pipette lines I3 in the sample batch shaking extraction module. About 5 ml of buffer solution is added to each sample cup 8. After the addition is completed, the sample tray 6 is rotated by the drive device I7 so that the lower end of the outlet of the three pipette lines I3 is aligned with the three sample cups 8 in the next sample group and then the solution is added. In this way, the drive device I7 is rotated 6 times to add buffer solution to all 18 sample cups 8. After the addition is completed, the reagent in the sample cup 8 is shaken and mixed by continuing to control the drive device I7. Shake for 1 min to 2 min to obtain the extract, and let it stand for 3 min to 5 min. (3) Control the lifting platform 17 in the batch quantitative transfer module of the extract to descend, so that the three pipettes II 13 are inserted into the three sample cups 8 respectively and the inlet of the pipettes II 13 is below the liquid surface and close to the bottom of the sample cup 8. The pipettes III 14 are aligned with the three adjacent cuvettes 12 respectively. At this time, the three liquid addition pumps II are started to add the extract in the three sample cups 8 into the three adjacent cuvettes 12 through the pipettes II 13, the liquid addition pumps II, and the pipettes III 14 respectively. (4) After the first group of 3 cuvettes 12 has been filled with liquid, the lifting platform 17 in the batch quantitative transfer module of the extract is raised so that the bottom surface of the 3 pipettes II 13 and the suction needle III is higher than the top surface of the sample cup 8, and the bottom surface of the pipettes III 14 and the injection needle IV is higher than the top surface of the cuvette. At this time, the pipettes II 13 are drawing air. By setting a sufficient time, the extract in the pipettes II 13 and III 14 is almost completely put into the cuvettes 12. At this time, the sample tray 6 and the turntable 10 are rotated by two motors respectively. When the bottom of the three pipettes II 13 is aligned with the 3 sample cups 8 in the second group of samples, the bottom of the three pipettes III 14 is aligned with the 3 cuvettes 12 in the second group. At this time, the lifting platform 17 is lowered again to add liquid to the cuvettes 12 in the second group. The control method is the same as above. The pretreatment process is completed when the 18 cuvettes 12 are added in 6 times.

[0033] The specific structure for implementing the above functions is as follows: A rapid pesticide residue detection pretreatment extraction and sampling device, including an operating table 1, such as... Figure 1 , Figure 3 As shown, from left to right, the operating table 1 is equipped with (1) a buffer solution batch quantitative sample addition module; (2) a sample batch shaking extraction module; (3) an extract batch quantitative transfer module; and (4) a sample tray transfer module. The above four modules are viewed from left to right as follows: (1) A buffer solution batch quantitative sample addition module for adding buffer solutions; The buffer batch quantitative sample addition module includes a buffer bottle 2, a pipette pump I for adding the buffer solution in the buffer bottle to the sample cup through a pipette line I, and a placement rack 4; like Figure 2 , Figure 4 As shown, one end of the inverted "L"-shaped placement rack 4 is inserted into the bottom surface of the operating table 1 and the two are fixed together with screws. Thus, the two sides of the buffer solution bottle 2 are restricted between the operating table 1 and the placement rack 4. Since the buffer solution bottle 2 is a glass bottle, the placement rack 4 restricts the buffer solution bottle 2 left and right to avoid tipping. Furthermore, a hook-shaped groove 5 is provided at the top of the placement rack 4 on the right side of the operating table 1. (2) Sample batch shaking extraction module; The sample batch shaking extraction module includes a circular sample tray 6 embedded in the operating table 1, a drive device I7 located in the operating table 1 and at the bottom of the sample tray 6 for driving the sample tray 6 to rotate, and a buffer batch quantitative sample addition module for adding the buffer solution in the buffer bottle 2 to the sample cup 8 through the pipetting tube I3. The sample tray 6 has 18 circularly arranged grooves. These grooves then expand outwards from the center point of the sample tray 6 in two concentric rings, each ring containing multiple grooves. The largest ring has 12 grooves, and the middle ring has 6 grooves. These grooves are used to hold sample cups 8 (for easy viewing). Figure 1 Only three sample cups 8 were placed in the middle (the three cups within the elliptical dotted line on the right are the ones containing sample cups 8), and the sample to be tested was placed in the sample cups 8. The sample was vegetables that had been rinsed to remove surface dirt, such as... Figure 1 As shown, the three sample cups 8 form a sample group ( Figure 1 The two dashed elliptical portions in the middle circle two groups), and there are a total of 6 groups of samples on the sample tray 6; The buffer batch quantitative sample addition module includes three pipette pumps I and a dosing rack 9. The dosing rack 9 is fixed on the operating table 1. The buffer solution in the buffer bottle 2 is added to three sample cups 8 one-to-one through three pipette lines I3 and three pipette pumps I. Each of the three pipette lines I3 is fixed on the dosing rack 9. The lower ends of the three outlets of the three pipette lines I3 are respectively aligned with the three sample cups 8. Then, each time the driving device I7 drives the sample tray 6 to rotate once, the top of the next group of three sample cups 8 is aligned with the three outlets of the three pipette lines I3. After the driving device I7 drives the sample tray 6 to rotate once and return to its original position, buffer solution has been added to the sample cups 8 of each group of samples. The buffer bottle 2, which is used to hold the buffer solution, is located on one side of the operating table 1. The pipette pump I can be fixed inside the operating table 1 or set on the operating table 1. There are no restrictions on its position in this case, as long as it can achieve liquid extraction.

[0034] The drive device I7 in the above-mentioned components is a stepper motor, which is purchased from the market. The drive shaft of the stepper motor is fixedly connected to the sample tray 6, thereby providing rotational power and angular positioning for the sample tray 6 through the stepper motor, so as to realize the shaking and mixing of reagents in the sample cup 8 and the accurate positioning relative to each sample group. The pipette pump I in the above components can be a peristaltic pump I, a micro-injection pump I, or a dosing metering pump I, preferably the peristaltic pump I; the addition volume can be controlled by changing the pipette line I3 with different inner diameters; when it is a liquid addition pipette pump I or a peristaltic pump I, it also includes a suction needle, and the buffer solution in the buffer bottle 2 is added to the sample cup 8 in sequence through the suction needle I, the pipette line I3, the pipette pump I, the pipette line I3, and the injection needle II; (3) Batch quantitative transfer module for extract; The batch quantitative transfer module for the extract includes a pipette line II 13, a pipette line III 14, and a dispensing pump II for drawing the mixed, shaken, and homogenized test reagent from each set of sample cups 8 into the cuvettes 12; and a power device 15 for driving the lifting platform 17 up and down. The a pipette lines II 13 and III 14 are fixed on the lifting platform 17. The lower end of the inlet of the a pipette line II 13 is aligned one-to-one with the a sample cups 8 in a set of samples, and the lower end of the outlet of the a pipette line III 14 is aligned one-to-one with the a adjacent cuvettes 12. The a dispensing pump II draws the test reagent from the a sample cups 8 in a set of samples into the a adjacent cuvettes 12 in one go through the a pipette lines II 13 and the a pipette lines III 14. The power unit 15 can be a stepper motor. The lifting platform 17 is fixed to the stepper motor to realize the up and down movement of the lifting platform 17, and further control whether the pipette line II 13 can draw the test reagent. The reason for realizing the up and down movement function is that after the liquid is drawn from a set of samples, if the sample tray 6 is rotated directly, the next set of sample cups 8 will scrape the pipette line I 3. Lifting it can avoid the above problem and reduce the problem of drug addition deviation caused by deformation of the pipette line I 3. Secondly, the descent of the lifting platform 17 can ensure that liquid can be drawn. Thirdly, the amount of liquid drawn each time is limited. For example, after drawing twice, the lifting platform 17 rises, and it will not be able to contact the liquid surface when drawing again. Therefore, the amount of liquid added each time is fixed. After rising, if the liquid is drawn again, it will be air drawn because it is no longer in contact with the liquid surface, until all the liquid in the pipette line III 14 is drawn into the cuvette 12. This reduces the problem of cross-contamination caused by mixing with the next set of samples. (4) Sample tray transfer module; The sample tray transfer module includes a turntable 10 embedded in the operating table 1 and a drive device II 11 fixed in the operating table 1 to drive the turntable 10 to rotate. The turntable 10 has 18 grooves arranged in a ring, and a total of 18 cuvettes 12 can be inserted into all the grooves. Regarding the drive device Ⅱ11 mentioned in component ① above, it is a motor, which is purchased from the market. The drive shaft of the motor is connected to the turntable 10, thereby driving the turntable 10 to rotate. Regarding the liquid addition pump II mentioned in component ② above, it can be a peristaltic pump II, a micro-injection pump II, or a drug metering pump II, preferably the peristaltic pump II; the addition volume can be controlled by changing the pipetting tubing II 13 and III 14 with different inner diameters and controlling the aspiration time of the pipetting tubing II 13; when it is liquid addition pump II or peristaltic pump II, it also includes a suction needle, and then the buffer solution in the sample cup 8 is added to the sample cup 8 in sequence through the suction needle III, the pipetting tubing II 13, the liquid addition pump II, the pipetting tubing III 14, and the injection needle IV.

[0035] Furthermore, the liquid pump II mentioned above can be fixed on the lifting platform 17 or placed on the sample tray 6, as long as it can realize liquid extraction and delivery. All pumps are purchased from the market.

[0036] Example 2:

[0037] The structure is as follows when the extract in sample cup 8 needs to be heated: Based on Example 1, each sample cup 8 is provided with a heating module at the bottom. The heating module includes a circular heating plate at the bottom of the sample tray 6. The heating plate is connected to a temperature controller at its bottom for controlling the temperature of the heating plate. The temperature controller is connected to a controller, and the controller controls the temperature value of the temperature controller. The heating module realizes the heating of the extract.

[0038] Example 3:

[0039] To prevent pipetting lines II 13 and III 14 from becoming blocked, based on Embodiment 1 or Embodiment 2, the aspiration needle III and the filter screen are connected by a limiting structure and an elastic element to achieve a buffer connection. The aspiration needle ensures the stability of the aspiration process, and the filter screen ensures the filtration effect of the aspiration process, thus preventing blockage of the pipetting lines II 13 and III 14.

[0040] Example 4:

[0041] To prevent the buffer solution from being added to sample cup 8 when there is no sample cup 8 directly below the outlet of pipette line I3; and to prevent the extract from being added to cuvette 12 when there is no cuvette 12 directly below the outlet of pipette line III14, sensors are installed on the dosing rack 9 and the lifting platform 17, respectively, based on any one of Examples 1-3. Specifically: The lifting platform 17 and the dosing rack 9 are each equipped with a sensor for detecting whether there is a sample cup 8 or a cuvette 12 below; Preferably, the sensor can be an optical sensor such as a laser sensor or an infrared reflective photoelectric sensor, with an infrared reflective photoelectric sensor being preferred. The principle is that when the infrared emitter emits infrared light, it encounters the cuvette 12 or sample cup 8 in its path and is reflected back by the cuvette 12 or sample cup 8. This reflected infrared light is detected by the receiver. When the cuvette 12 or sample cup 8 is not present, the receiver receives a strong infrared signal, while when the cuvette 12 or sample cup 8 approaches the sensor, the received infrared signal becomes weaker.

[0042] Example 5:

[0043] To lay the groundwork for testing processes beyond those described in this application, we have established the following structure based on Examples 1-4: The power unit 15 also includes a device for rotating the lifting platform 17. Therefore, the power unit 15 can be a combination of two motors, in which case, from top to bottom, it consists of the lifting platform 17, a motor VII for rotating the lifting platform 17 circumferentially, the motor VII at the bottom that drives the upper part of it, and a motor VIII for moving the lifting platform 17 up and down. Alternatively, it can be a combination of the lifting platform 17, a mini cylinder, and a motor, where the motor is used for circumferential rotation and the cylinder is used for moving the platform up and down. This application does not impose any limitations, as long as the above functions can be achieved. The reason for achieving the rotation function is twofold: first, it serves as a base when the turntable 10 is transferred to the testing instrument later, so as not to obstruct the area directly above the turntable 10; second, it allows for adjustment so that a pipettes II 13 can be inserted into a sample cups 8 respectively. The turntable 10 adopts a fully extractable structure, meaning that all turntables 10 and cuvettes 12 on them can be extracted at once and transferred to subsequent detection instruments for testing. This rapid pesticide residue detection pretreatment extraction and sampling device is only used for batch addition of buffer solution and batch processing, followed by transfer to detection instruments other than those described in this application. Specifically, this can be achieved through the following structure: First, the lifting platform 17 adopts a slender structure. After the solution to be tested is added to the cuvettes 12, during the upward extraction process of the turntable 10, the power device 15 drives the lifting platform 17 to rotate, preferably without obstructing the top of the turntable 10. Then, the drive device II 11 is fixed inside the operating table 1. The turntable 10 and the drive shaft of the drive device II 11 are detachably connected. The detachable connection can be achieved by providing a limiting block 18 on the drive shaft. The bottom surface of the turntable 10 The turntable 10 is provided with a limiting groove 19 that matches the shape of the limiting block 18. The limiting block 18 is inserted into the limiting groove 19 and the drive shaft is inserted into the turntable 10. Due to the limiting effect of the limiting block 18, the drive device II 11 can drive the turntable 10 to rotate circumferentially without affecting the lifting process. The center of the top of the turntable 10 is provided with a threaded hole 20. After the screw with the nut is screwed into the threaded hole 20 of the turntable 10, there is a certain gap between the bottom surface of the nut and the top surface of the turntable 10. When transferring to the testing instrument later, the nut can be lifted manually. In order to avoid contamination, a robotic arm can be inserted into the gap between the turntable 10 and the nut. After locking, the robotic arm lifts upward and then the turntable 10 and the cuvette 12 on it are installed into the matching testing instrument. This forms a complete set of rapid pesticide residue detection lines that reduce contamination.

[0044] Example 6:

[0045] To improve automation, based on embodiments 1-5, this device incorporates the controller and a battery to provide power. The sensor sends a signal to the controller, triggering the next dosing instruction only when the sample cup 8 or cuvette 12 is present. Furthermore, the operating table 1 has at least five function keys 16: "Start / Stop," "Cleaning," "Lifting / Lowering," "Turntable 10 Movement," and "Number of Channels." All of these function keys 16 are electrically connected to the controller, and the drive device I 7, drive device II 11, power device 15, and sensor are electrically connected to the controller. Various start / stop states can be controlled via these five function keys 16. The specific operation process of the five function keys 16 is as follows: (1) "Start / Stop" button: In standby mode, press and hold for 3 seconds to start the separation program. After the separation is completed, the program will return to standby mode. During program operation, press the button briefly to pause the program. Press it again briefly to resume the program. In the paused state, press and hold the button for 3 seconds to cancel the separation task and return to standby mode. (2) "Cleaning" button: When the cleaning button is pressed in standby mode, the pipeline cleaning task will be executed; (3) "Lift / Lower" button: In standby mode, press and hold the lift / lower button for 1 second, the lift / lower rod will start to rise, then fall after reaching the top, and then start to rise again after reaching the lowest point. Release the button and the lift / lower rod will stop moving. (4) "Turntable 10 movement" button: Press briefly, and turntable 10 will rotate 1 / 3 of a turn, making it easier to put away measuring cups and cuvettes 12; (5) "Channel Quantity" button: This button adjusts the number of channels from 1 to 18. The channel number cycles between 1 and 18. A short press increments the channel number by 1 until it reaches 18. Another short press resets the channel number to 1. A long press for more than 1 second rapidly increases the channel number (the displayed value increases by 1 every 0.25 seconds). Releasing the button stops the change in value, and the displayed value indicates the number of measuring cups and cuvettes 12 placed on the dispensing turntable 10 and cuvette 12 (starting from channel 1 and proceeding sequentially until the channel corresponding to the displayed value is reached; for example, if the displayed value is 3, then liquid needs to be added to the measuring cups and cuvettes 12 for channels 1, 2, and 3). Furthermore, the amount of feed can be controlled by setting the rotation angle and dwell time of drive device I7, drive device II11, and power device 15 through the controller. They can all adopt a fixed mode by default, or they can be manually controlled by adding a switch. Furthermore, based on this application, a display screen can also be set, which is electrically connected to the controller through a touch screen display screen to realize control.

[0046] Based on the pretreatment and testing steps in the GB / T 5009.199 standard, we have made slight modifications to this process as follows: (1) Preparation: Place the corresponding number of extraction cups (with samples already placed in the extraction cups, but no sample in cup 1) and empty cuvettes 12 in the "sample tray rack 6" and "sample liquid tray (rotary tray 10)" according to the number of samples to be tested, and a sufficient volume of extraction buffer solution, and set the extraction quantity.

[0047] (2) Operation: One-button start to launch the "Automatic Pipette Extraction" program: (Function buttons involved: [Start / Stop]) ① Use the "extraction pump" to transfer 5 ml of buffer solution into sample cup 8; ② Shake the “sample tray 6” for 1 minute to mix thoroughly; ③ The "sample transfer needle" descends to near the bottom of the inner wall of sample cup 8; ④ After the “sample transfer pump” transfers 2ml of sample solution, the “sample transfer aspiration needle” is raised above the rim of the upper cup.

[0048] ⑤ The “sample transfer pump” pumps 2 ml of sample solution into cuvette 12.

[0049] ⑥ The "sample tray 6" rotates sequentially according to the extraction quantity, while the "sample liquid tray (rotary disk 10)" rotates synchronously. After completion, the next pipetting cycle is executed. [Steps ③, ④, and ⑤] ⑦ After completing all pipetting, stop, reset, and emit a beeping sound.

[0050] Routine maintenance: (1) Cleaning: Place purified water in buffer bottle 2, place 3 empty sample cups 8 in sample tray rack 6, and place 3 empty cuvettes 12 in turntable 10. Start the "cleaning" function and use the "extraction pump" to transfer 10 ml of buffer solution into sample cup 8. Rinse pipette I 3 once. Use the "sample transfer pump" to repeatedly clean pipette II 13 and pipette III 14 with purified water in sample cup 8 three times in both directions. When rotating forward, add about 0.5 ml of liquid to cuvette 12 each time.

[0051] (2) Lifting: Use the [Lifting] function when picking up or putting down the sample cup 8 and cuvette 12 (only lift the "sample transfer aspiration needle" part).

[0052] (3) Turntable 10: Use the [Turntable 10] function when picking up and putting down sample cup 8 and cuvette 12.

[0053] (4) Quantity: Set the quantity before each extraction begins.

[0054] Based on the pretreatment and testing steps in the GB / T 5009.199 standard, we have made slight modifications to this process as follows: (1) Preparation: Place the corresponding number of extraction cups (with samples already placed in the extraction cups, but no sample in cup 1) and empty cuvettes 12 in the "sample tray rack 6" and "sample liquid tray (rotary tray 10)" according to the number of samples to be tested, and a sufficient volume of extraction buffer solution, and set the extraction quantity.

[0055] (2) Operation: One-button start to start the "Automatic Pipette Extraction" program: (Function buttons involved: [Start / Stop]) ① Use “Extraction Pipette I” to transfer 5 ml of buffer solution into sample cup 8; ② Shake the “sample tray 6” for 1 minute to mix thoroughly; ③ The “liquid aspiration needle III” descends to near the bottom of the inner wall of sample cup 8; ④ After “liquid pump II” transfers 2ml of sample solution, “liquid injection needle IV” is raised above the rim of sample cup 8.

[0056] ⑤ Use “Liquid Addition Pump II” to pump 2 ml of sample solution into cuvette 12.

[0057] ⑥ The "sample tray 6" rotates sequentially according to the extraction quantity, while the "sample liquid tray (rotary disk 10)" rotates synchronously. After completion, the next pipetting cycle is executed. [Steps ③, ④, and ⑤] ⑦ After completing all pipetting, stop, reset, and emit a beeping sound.

[0058] Example 7:

[0059] Examples 1-6 are all for the pretreatment process of high-throughput enzyme inhibition pesticide residue detection. For other pesticide residue detection pretreatment processes, when it is necessary to add several different buffer solutions to the sample cup, multiple buffer solutions can be added by changing different buffer bottles 2 in sequence, and each buffer bottle 2 contains a different buffer solution.

[0060] Example 8:

[0061] For the 24-channel detector, based on Examples 1-7, the structure of the sample tray 6 and the turntable 10 is modified so that each has 24 grooves for inserting sample cups 8 and cuvettes 12, with 3 samples per group, for a total of 8 groups, allowing for 8 liquid additions to meet the requirements. Alternatively, the overall structure can be modified, for example, by installing 4 pipetting lines II 13, III 14, and I 3 on the lifting platform 17 and the dosing rack 9, with 4 pumps corresponding to each line. This allows liquid to be added to 4 sample cups 8 and cuvettes 12 at once, for a total of 6 additions, thus enabling a faster pretreatment process.

[0062] The above description provides a detailed account of one embodiment of the present invention. However, this description is merely a preferred embodiment and should not be construed as limiting the scope of the present invention. All equivalent variations and improvements made within the scope of the claims of the present invention should still fall within the patent coverage of the present invention.

Claims

1. A pretreatment extraction and sampling device for rapid detection of pesticide residues, including an operating table, characterized in that: The operating platform is equipped with a sample liquid tray transfer module, an extract batch quantitative transfer module, a sample batch shaking extraction module, and a buffer solution batch quantitative sample addition module for adding buffer solution. The buffer batch quantitative sample addition module includes a buffer bottle and a pipette pump I for adding the buffer solution in the buffer bottle into the sample cup through pipette I; The sample batch shaking extraction module includes a circular sample tray frame embedded in the operating table and a driving device I located inside the operating table and at the bottom of the sample tray frame for driving the sample tray frame to rotate. The sample tray frame is provided with a plurality of circularly arrayed grooves, and the grooves are arranged in at least one ring with multiple grooves in each ring, emanating from the center point of the sample tray frame. The grooves are used to place sample cups, and the sample to be tested is placed in the sample cups. a sample cups form a sample group, and there are a total of b sample groups on the sample tray frame, where a and b are at least 2. The buffer batch quantitative sample addition module includes a pipette pumps I. The buffer solution in the buffer bottle is added to a sample cups one-to-one through a pipette line I and a pipette pumps I. The lower ends of a outlets of a pipette line I are respectively aligned with a sample cups. Then, each time the driving device I drives the sample tray to rotate, the top of the next group of a sample cups is aligned with a outlet of a pipette line I. The sample tray transfer module includes a turntable embedded in the operating table and a drive device II fixed in the operating table to drive the turntable to rotate. The turntable has ≥b grooves arranged in a ring, and cuvettes are inserted into the grooves. The batch quantitative transfer module for the extract includes *a* pipette lines II, *a* pipette lines III, and *a* dispensing pumps II for drawing the test reagent into the cuvettes, and a power device for driving the lifting platform up and down. *a* pipette lines II and *a* pipette lines III are fixed to the lifting platform. The lower end of the inlet of *a* pipette lines II is aligned one-to-one with *a* sample cups in a sample group, and the lower end of the outlet of *a* pipette lines III is aligned one-to-one with *a* adjacent cuvettes. The *a* dispensing pumps II, through *a* pipette lines II, draw the test reagent from the *a* sample cups in a sample group into the *a* adjacent cuvettes in one continuous operation via *a* pipette lines III.

2. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 1, characterized in that: One end of the inverted "L"-shaped placement rack is inserted into the bottom of the operating table and the two are fixed together with screws, thereby restricting the two sides of the buffer bottle between the operating table and the placement rack; The buffer batch quantitative sample addition module includes a dosing rack, which is fixed on the operating table, and each of the a pipette lines I is fixed on the dosing rack.

3. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 1 or 2, characterized in that: The groove is provided in at least two concentric rings, with multiple grooves in each ring, extending outwards from the center point of the sample tray. After the driving device I drives the sample tray to rotate once and return to its original position, buffer solution is added to the sample cup of each sample group. The number of a is 3, and the line connecting the outlets of the 3 pipettes I forms an isosceles triangle; the number of b is 18, and the drive device I can add buffer solution to all 18 sample cups by rotating 6 times.

4. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 3, characterized in that: The driving device I is a stepper motor, and the drive shaft of the stepper motor is fixedly connected to the sample tray, thereby providing rotational power and angular positioning for the sample tray through the stepper motor; The drive device II is a motor, and the transmission shaft of the motor is connected to the turntable, thereby driving the turntable to rotate; The pipette pump I is a peristaltic pump I, a micro-injection pump I, or a dosing metering pump I; when it is a liquid addition pipette pump I or a peristaltic pump I, it also includes a suction needle, and the buffer solution in the buffer bottle is added to the sample cup in sequence through the suction needle I, the pipetting tubing I, the pipette pump I, and the injection needle II; The power unit is a stepper motor or a cylinder. The lifting platform is fixed on the stepper motor or cylinder to realize the up and down movement of the lifting platform, and further control whether the pipetting line II can draw the test reagent.

5. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 1 or 4, characterized in that: The power unit also includes a device for driving the lifting platform to rotate. The power unit is a combination of two motors. From top to bottom, the motors are: the lifting platform, motor VII for driving the lifting platform to rotate circumferentially, motor VII at the bottom that drives the upper part of it, and motor VIII for moving the lifting platform up and down. Alternatively, the power unit can be a combination of the lifting platform, a mini cylinder, and a motor. The motor is used for circumferential rotation, and the cylinder is used for driving up and down movement.

6. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 5, characterized in that: The liquid addition pump II is a peristaltic pump II, a micro-injection pump II, or a drug metering pump II; when it is a liquid addition pump II or a peristaltic pump II, it also includes a suction needle, and then the buffer solution in the sample cup is added to the sample cup in sequence through the suction needle III, the pipetting line II, the liquid addition pump II, the pipetting line III, and the injection needle IV; The suction needle III and the filter screen are connected by a limiting structure and an elastic element for buffering.

7. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 6, characterized in that: Both the lifting platform and the dosing rack are equipped with a sensor for detecting whether there is a sample cup or cuvette below.

8. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 1, 2, 4, 6, or 7, characterized in that: The turntables adopt a fully extractable structure, meaning that all the turntables and the cuvettes on them can be extracted at once and transferred to the subsequent testing instrument for detection. This is achieved through the following structure: The lifting platform has a slender structure. After the solution to be tested is added to the cuvette, when the turntable is lifted upward, the power device drives the lifting platform to rotate without obstructing the top of the turntable. The drive device II is fixed inside the operating table, and the turntable is detachably connected to the drive shaft of the drive device II. The center of the top of the turntable has a threaded hole. After the screw with a nut is screwed into the threaded hole of the turntable, there is a certain gap between the bottom surface of the nut and the top surface of the turntable.

9. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 8, characterized in that: The turntable is detachably connected to the drive shaft of the drive device II. The detachable connection is achieved by providing a limiting block on the drive shaft and a limiting groove matching the shape of the limiting block on the bottom surface of the turntable. The limiting block is inserted into the limiting groove and the drive shaft is inserted into the turntable. Due to the limiting effect of the limiting block, the drive device II drives the turntable to rotate circumferentially without affecting the lifting process. The inlet lines of pipetting line II form an isosceles triangle; the outlet lines of pipetting line III form an arc. Each of the sample cups is equipped with a heating module at the bottom. The heating module includes a circular heating plate located at the bottom of the sample tray. The heating plate is connected to a temperature controller at its bottom for controlling the temperature of the heating plate. The temperature controller is connected to a controller, which controls the temperature value of the temperature controller.

10. The pretreatment extraction and sampling device for rapid detection of pesticide residues according to claim 9, characterized in that: This device has a built-in controller and a battery that provides power to the device. The sensor sends signals to the controller. The operating panel has at least 5 function keys, namely: "Start / Stop", "Cleaning", "Lifting", "Turntable Movement", and "Number of Channels". All of the above function keys are electrically connected to the controller, and the drive device I, drive device II, power device, and sensor are electrically connected to the controller.

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