NON-THERAPEUTIC COSMETIC PROCESS FOR REDUCING WRINKLES ON A SKIN SURFACE, KIT FOR IMPLEMENTING THE PROCESS, AND PROCESS FOR USING A KIT

Abstract

A non-therapeutic cosmetic process for reducing wrinkles on a skin surface, a kit for implementing the process, and a method for using a kit. The present invention relates to a non-therapeutic cosmetic process for treating a skin surface, said process comprising the combination of a medium with ultrasonic waves, wherein the medium includes a suitable cosmetic product. One aspect of the present invention relates to a non-therapeutic cosmetic process for reducing wrinkles on a skin surface, the process comprising the steps of applying an ultrasonic transmission medium to the skin surface to be treated, wherein the medium includes cosmetic active ingredients / formulas, and applying ultrasonic waves to the skin surface with the applied medium. The ultrasonic waves produce an acoustic output.The ultrasonic waves have a frequency of 10 kHz to 50 MHz, preferably 30 kHz to 20 MHz, and preferably 1 MHz to 10 MHz. The ultrasonic waves are generated in such a way that they propagate through the skin to a maximum depth of 3 mm from the skin surface and generate a rise in skin temperature at the application areas. See Figure 1 for abbreviations.

Description

Title of the invention: NON-THERAPEUTIC COSMETIC METHOD FOR REDUCING WRINKLES ON A SKIN SURFACE, KIT FOR CARRYING OUT THE METHOD, AND METHOD FOR USING A KIT Field of invention

[0001] The present invention relates to a non-therapeutic cosmetic method for treating a skin surface, said method comprising combining a medium with ultrasonic waves, wherein the medium includes a suitable cosmetic product. More specifically, the method aims at reducing wrinkles. The present invention also relates to a kit for carrying out the method and a method of using the kit. Background of the invention

[0002] The application of cosmetic actives / formulas to the skin is known as a method for improving wrinkles on the skin. A non-limiting example of cosmetic actives / formulas is hyaluronic acid. Hyaluronic acid is a natural compound found in many human tissues. Human tissues include the skin, synovial fluid of joints, vitreous fluid of the eye, and cartilage scaffolding, etc. Among these tissues, the largest amount of hyaluronic acid in the body is found in the skin.

[0003] With skin aging and repeated exposure to the sun's ultraviolet rays, dermal cells in the skin decrease their production of hyaluronic acid. Additional background information on skin aging can be found in the paper "Hyaluronic acid, A key molecule in skin aging" published in Dermato-Endocrinology 4:3, 253-258; July-December 2012.

[0004] Hyaluronic acid is used in moisturizing and / or anti-aging products in the form of creams, lotions, toners, masks, etc. Hyaluronic acid may include hyaluronic acid salts such as sodium hyaluronate and potassium hyaluronate. Cosmetic products including hyaluronic acid are applied topically to the skin. Therefore, a limited amount of hyaluronic acid penetrates the skin, which can cause problems when the effects on wrinkles and skin texture are limited or short-lived.

[0005] In order to enhance the effects, hyaluronic acid or a hyaluronic acid-based composition is known to be used as a dermal filler. During use, the hyaluronic acid or hyaluronic acid-based composition can be injected into the skin to fill superficial lines, folds and wrinkles. However, these hyaluronic acid-based fillers are Typically injected with a needle just below the skin's surface, primarily into the dermis, and as a result, problems such as needle phobia, risk of infection, and sterilization difficulties may arise.

[0006] In order to overcome the disadvantages associated with the use of needles, alternative skin tightening systems have been introduced. One such skin tightening system involves using a medicament containing a gel applied as a medium to deliver energy waves to the skin and thus the energy waves can pass through the skin.

[0007] WO 2019 / 164 836 A1 (Patent Document 1) discloses the use of high-intensity focused ultrasound (HIFU) prior to injecting a dermal filler compound and a fat-reducing compound into the dermis of the skin using a needle syringe.

[0008] Document KR 2015 / 0 101 965 A (patent document 2) also discloses a use of the HIFU system for a skin tightening or facial smoothing procedure at the level of the dermis and layers deeper than the dermis, using an ultrasound gel as a medium for transmitting energy to the skin. The HIFU device comprises interchangeable probe heads having different penetration depths.

[0009] Document KR 2016 / 0 139 518 A (patent document 3) discloses the delivery of HIFU to a predetermined depth of the skin surface using a gel medium for aesthetic treatment in layers deeper than the dermis of the skin, generating a local thermal effect during the treatment. Summary of the invention Technical problem to be solved

[0010] However, HIFU therapy as disclosed in Patent Documents 1 to 3 may be invasive so that a recipient feels pain. Furthermore, exposure to HIFU for a long period of time may induce a significant increase in temperature and excessive thermal effects on the skin. Furthermore, with respect to application to facial skin, a region to be targeted for HIFU therapy may be limited. Since HIFU may be invasive, the devices used in HIFU therapy may only be handled by experts or dermatologists, and they are considered a form of medical treatment or equivalent.

[0011] Therefore, a new solution to improve wrinkles in a less invasive way in salons and at home is needed.

[0012] Accordingly, a less invasive process that can be used in salons and at home and achieves noticeable cosmetic benefits for the skin is necessary.

[0013] The present invention aims to provide a method combining a medium including cosmetic actives / formulas and ultrasonic waves for facial enhancement, which are of lower energy than HIFU, a kit for implementing the method, and a method of using a kit. Ways to solve the problem

[0014] In order to solve the above-mentioned problem, one aspect of the present invention relates to a non-therapeutic cosmetic method for reducing wrinkles on a skin surface, the method comprising the steps of:

[0015] applying an ultrasound transmission medium to the surface of the skin to be treated, wherein the medium includes cosmetic actives / formulas and

[0016] applying ultrasonic waves to the skin surface with the applied medium,

[0017] wherein the ultrasonic waves produce an acoustic output, wherein the ultrasonic waves have a frequency of 10 kHz to 50 MHz, preferably 30 kHz to 20 MHz, and more preferably 1 MHz to 10 MHz, and

[0018] wherein the ultrasonic waves are generated such that the ultrasonic waves propagate into the skin to a maximum depth of 3 mm from the surface of the skin and generate a rise in temperature of the skin at the application areas.

[0019] According to one aspect of the present invention, the ultrasonic waves may be unfocused ultrasonic waves. In this case, the temperature of the skin surface may be less than or equal to 50°C.

[0020] According to one aspect of the present invention, the ultrasonic waves may be focused ultrasonic waves and the ultrasonic waves may generate a local rise in temperature of the skin at the focal area in a short time. In this case, the temperature of the skin at the focal area when the ultrasonic waves are applied to the skin may be greater than 40°C and less than or equal to 65°C, preferably greater than 40°C and less than or equal to 60°C, and preferably greater than 40°C and less than or equal to 50°C.

[0021] According to one aspect of the present invention, the ultrasonic waves may be pulsed and focused ultrasonic waves. In this case, the pulse energy of the ultrasonic waves may be equal to or greater than 0.001 J and less than 10 J, preferably equal to or greater than 0.01 J and less than 5 J.

[0022] According to one aspect of the present invention, the ultrasonic waves may be pulsed ultrasonic waves. In this case, the pulse duration of the ultrasonic waves may be less than or equal to 150 ms, preferably less than or equal to 80 ms, preferably from 4 ms to 80 ms, more preferably from 4 ms to 70 ms, and even more preferably from 4 ms to 50 ms.

[0023] According to one aspect of the present invention, the ultrasonic waves may be focused and pulsed ultrasonic waves. In this case, the peak power of the ultrasonic waves may be less than 80 W, preferably less than or equal to 65 W, preferably less than or equal to 15 W, more preferably less than or equal to 5 W, and even more preferably from 0.1 W to 2 W.

[0024] According to one aspect of the present invention, the ultrasonic waves may be continuous waves. In this case, the duration of the ultrasonic waves may be less than or equal to 1 s.

[0025] According to one aspect of the present invention, the ultrasonic waves can be propagated towards one or more layers of the skin, which are the epidermis and / or the dermis.

[0026] According to one aspect of the present invention, the cosmetic active ingredients / formulas may include at least one element chosen from the group consisting of hyaluronic acid, polyols, vitamins, peptides, ceramides, growth factors.

[0027] According to one aspect of the present invention, the cosmetic actives / formulas may include hyaluronic acid. A concentration of the hyaluronic acid may be greater than 0.1% relative to the total mass of the medium.

[0028] According to one aspect of the present invention, a molecular mass of hyaluronic acid may be between 5 kDa and 20 MDa, preferably between 10 kDa and 2 MDa.

[0029] According to one aspect of the present invention, a dynamic viscosity of the medium may be between 4 Pas and 50 Pas.

[0030] Another aspect of the present invention relates to a kit for implementing the method according to claim 1, the kit comprising:

[0031] a device, comprising:

[0032] an ultrasonic wave unit which comprises a transducer which emits ultrasonic waves;

[0033] a housing that houses the ultrasonic wave unit; and

[0034] a head, and

[0035] a medium for perceiving cosmetic benefits of the skin and for propagating ultrasonic waves, wherein the medium includes cosmetic actives / formulas.

[0036] Another aspect of the present invention relates to a method of using a kit, the kit comprising:

[0037] a device, comprising:

[0038] an ultrasonic wave unit which comprises a transducer which emits ultrasonic waves;

[0039] a housing that houses the ultrasonic wave unit; and

[0040] a head,

[0041] a medium for perceiving cosmetic benefits of the skin and for propagating ultrasonic waves, wherein the medium includes cosmetic actives / formulas;

[0042] wherein the method comprises the steps of:

[0043] applying the medium to a skin surface;

[0044] generating the ultrasonic waves by means of the ultrasonic wave unit;

[0045] contacting the head with the skin or the medium on the skin; and

[0046] propagate the ultrasonic waves through the medium to the skin;

[0047] wherein the ultrasonic waves are focused and pulsed ultrasonic waves;

[0048] wherein the ultrasonic waves have a frequency of 10 kHz to 50 MHz, preferably from 30 kHz to 20 MHz, and preferably from 1 MHz to 10 MHz;

[0049] wherein the ultrasonic waves have an energy greater than or equal to 0.001 J and less than 10 J, preferably greater than or equal to 0.01 J and less than 5 J; and

[0050] wherein the temperature of the skin at the focal zone when applying the ultrasonic waves to the skin is greater than 40°C and less than or equal to 65°C, preferably greater than 40°C and less than or equal to 60°C, and preferably greater than 40°C and less than or equal to 50°C. Effects of the invention

[0051] One aspect of the present invention may relate to a method combining a medium including cosmetic actives / formulas and ultrasound waves for facial enhancement, which are of lower energy than HIFU, a kit for carrying out the method, and a method of using a kit. Brief description of the drawings

[0052] [Fig-1] [Fig. 1] is a functional diagram showing the method of reducing wrinkles on the skin surface according to one embodiment of the invention.

[0053] [Fig.2] [Fig.2] is a schematic view showing the configuration of a kit according to one embodiment of the invention.

[0054] [Fig.3] [Fig.3] is a functional diagram showing a method of using the kit according to one embodiment of the invention. Embodiments of the invention

[0055] < Method for reducing wrinkles on a skin surface>.

[0056] A method for reducing wrinkles on a skin surface according to one embodiment of the invention will be described. The method is a non-therapeutic cosmetic method. [Fig. 1] is a block diagram showing the method for reducing wrinkles on a skin surface according to one embodiment of the invention. The method for reducing wrinkles on a skin surface 100 includes a block 110 and a block 120.

[0057] In one embodiment of the invention, in block 110, the step of applying a ultrasonic transmission medium on the surface of the skin to be treated is carried out. The medium includes active ingredients / cosmetic formulas.

[0058] In one embodiment of the invention, at block 120, the step of applying ultrasonic waves to the surface of the skin with the applied medium is performed.

[0059] In one embodiment of the invention, the ultrasonic waves produce an acoustic output. The ultrasonic waves may be unfocused and / or focused ultrasonic waves. The ultrasonic waves may also be pulsed and / or continuous ultrasonic waves.

[0060] In one embodiment of the invention, the ultrasonic waves have a frequency of 10 kHz to 50 MHz. Preferably, the ultrasonic waves have a frequency of 30 kHz to 20 MHz, and more preferably 1 MHz to 10 MHz. The ultrasonic waves may have a frequency of 3 MHz to 9 MHz and may have a frequency of 5 MHz to 9 MHz. When the ultrasonic waves have a frequency within such a range, skin wrinkles can be improved in an effective and less invasive manner.

[0061] In one embodiment of the invention, the ultrasonic waves are generated such that the ultrasonic waves propagate in the skin to a maximum depth of 3 mm from the surface of the skin and generate a rise in temperature of the skin at the application areas. The ultrasonic waves propagate in one or more layers of the skin, which are the epidermis and / or the dermis.

[0062] In another embodiment of the invention, the ultrasonic waves are unfocused ultrasonic waves. In this case, the temperature of the skin surface is less than or equal to 50°C. When the temperature of the skin surface is within such a range, the method is bearable / imperceptible in the skin for the subject being treated using the method.

[0063] In another embodiment of the invention, the ultrasonic waves are focused ultrasonic waves, and the ultrasonic waves generate a local temperature rise of the skin surface at the focal zone in a short time. The expression "short time" here means much shorter than 1 s, for example 40 ms. In the case where the ultrasonic waves are focused ultrasonic waves, the temperature of the skin at the focal zone when the ultrasonic waves are applied to the skin is greater than 40°C, and less than or equal to 65°C, preferably greater than 40°C, and less than or equal to 60°C, and preferably greater than 40°C, and less than or equal to 50°C. When the skin temperature at the focal area is within such a range, the process is bearable / unnoticeable locally in the skin for the subject being treated using the process.Since known HIFU devices have a focal temperature of approximately . 55°C to 65°C, the method of one embodiment of the invention has a lesser thermal effect on the skin and is less invasive.

[0064] In another embodiment of the invention, the ultrasonic waves are pulsed and focused ultrasonic waves. In this case, the ultrasonic waves have a pulse energy of 0.001 J or more, and less than 10 J, preferably 0.01 J or more, and less than 5 J, preferably 0.01 J or more, and less than 1.2 J, and more preferably 0.01 J or more, and less than 0.7 J. The focused ultrasonic waves may have a pulse energy of more than 0.1 J and less than 0.7 J, and preferably more than 0.3 J and less than 0.7 J. When the ultrasonic waves have a pulse energy in such a range, the load on the skin is reduced and the method of an embodiment of the invention is less invasive.

[0065] In another embodiment of the invention, the ultrasonic waves are focused and pulsed ultrasonic waves. In this case, the peak power of the ultrasonic waves is less than 80 W, preferably 65 W or less, preferably 15 W or less, more preferably 5 W or less, and even more preferably 0.1 W to 2 W. When the peak power of the ultrasonic waves is within such a range, the method can become less invasive and also achieve a cosmetic effect.

[0066] In yet another embodiment of the invention, the ultrasonic waves are pulsed ultrasonic waves. In this case, the pulse duration of the ultrasonic waves is 150 ms or less, preferably 80 ms or less, preferably 4 ms to 80 ms, more preferably 4 ms to 70 ms, and even more preferably 4 ms to 50 ms. When the pulse duration of the ultrasonic waves is within such a range, the method can become less invasive and also achieve a cosmetic effect.

[0067] Furthermore, although embodiments using only focused ultrasound waves or pulsed ultrasound waves have been described, it goes without saying that the invention is not limited to these embodiments. Preferably, both focused and pulsed ultrasound waves are used in the method. The use of focused and pulsed ultrasound waves allows a large amount of energy to propagate to a limited area of ​​the skin in a short time. In other words, the method using focused and pulsed ultrasound waves has good spatio-temporal selectivity. It should be noted that in this embodiment, the temperature rises significantly to 65°C, but the method makes it bearable / imperceptible locally in the skin.

[0068] In yet another embodiment of the invention, the ultrasonic waves are continuous waves. In this case, the duration of the ultrasonic waves is 1 s or less.

[0069] As described above, the medium is a composition including active ingredients / formulas cosmetics. The cosmetic actives / formulas may include hyaluronic acid and / or its derivatives. In exemplary embodiments, the hyaluronic acid helps smooth and plump the skin. In some embodiments, the hyaluronic acid is in the form of hydrolyzed hyaluronic acid, cross-linked hyaluronic acid, acetylated hyaluronic acid, and a grafted hyaluronic acid polymer. In some embodiments, the hyaluronic acid is in the form of sodium hyaluronate or potassium hyaluronate. The hyaluronic acid may include a plurality of hyaluronic acid salts. The cosmetic actives / formulas are preferably a hyaluronic acid solution. The hyaluronic acid solution is preferably an aqueous hyaluronic acid solution.In one embodiment, water is present in the cosmetic actives / formulas at a concentration, by weight, relative to the total weight of the composition, of about 30% or more, alternatively about 40% or more, alternatively about 50% or more, alternatively about 85% or less, alternatively about 30% to about 85%, alternatively about 40% to about 80%, alternatively about 50% to about 75%, alternatively about 55% to about 75%, alternatively about 60% to about 70%, or any suitable value, range or sub-range thereof. Thus, water may be present in an amount of approximately 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%. 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, to about 85%. The environment can have the gel form.

[0070] In one embodiment of the invention, a viscosity of the hyaluronic acid solution depends on a molecular weight and a concentration of the hyaluronic acid. The concentration of the hyaluronic acid is preferably greater than 0.1% relative to the total mass of the medium. The concentration of the hyaluronic acid is preferably between 1.5% and 3% relative to the total mass of the medium. The use of the concentration of the hyaluronic acid in such a range makes it possible to effectively improve skin wrinkles.

[0071] In one embodiment of the invention, the molecular weight of hyaluronic acid is preferably between 5 kDa and 20 MDa. The molecular weight of hyaluronic acid is preferably between 10 kDa and 2 MDa and more preferably between 100 kDa and 2 MDa. The use of the molecular weight of hyaluronic acid in such a range increases the effect of hyaluronic acid on the skin and makes it possible to effectively improve epidermal wrinkles of the skin.

[0072] A high concentration or high molecular weight medium may have a high viscoelastic property. In one embodiment of the invention, the The dynamic viscosity of the medium is preferably between 4 Pas and 50 Pas, preferably between 20 Pas and 40 Pas. The use of dynamic viscosity in such a range allows the medium not to flow but to remain easily on the skin. As a result, skin wrinkles can be effectively improved.

[0073] In another embodiment of the invention, the medium may include skin care actives relating to benefits such as anti-aging, whitening, moisturizing and sun protection, etc. which are distinct from hyaluronic acid. Non-limiting examples of the other skin care actives include polyols, vitamins, peptides, ceramides and growth factors, etc.Other examples of the skin care actives may include retinoids which include retinol and its esters, retinal, retinoic acid and its derivatives, retinoids, and in particular those described in FR 2 570 377, EP 0 199 636, EP0 325 540 and EP 0 402 072, α-hydroxy acids such as glycolic or mandelic acid, [3-hydroxy acids, α-keto acids, [3-keto acids, peroxides such as benzoyl peroxide, free radical scavengers such as superoxide dismutase, selenium, zinc, beta-carotenes, and tightening polymers of natural or synthetic origin.

[0074] In exemplary embodiments, the tensioning polymers provide rapid or immediate tensioning of the skin. The tensioning polymers may include, but are not limited to, pullulan, polyvinylpyrrolidone (PVP), and combinations thereof.

[0075] In yet another embodiment of the invention, the cosmetic actives / formulas may include at least one element selected from the group consisting of hyaluronic acid, polyols, vitamins, peptides, ceramides, growth factors, and their derivatives, without being limited thereto.

[0076] (Vitamins)

[0077] Vitamins may include, but are not limited to, vitamin B3, vitamin C, vitamin E, vitamin F, and derivatives thereof. Examples of vitamin B3 derivatives include:

[0078] - nicotinic acid esters, such as those bearing the following INCI names: Nia- cinamide Ascorbate, Niacinamide Glycolate, Niacinamide Hydroxybenzoate, Nia-cinamide Hydroxycitrate, Niacinamide Lactate, Niacinamide Malate, Niacinamide Mandalate, Niacinamide Salicylate, Niacinamide Thioctate, supplied by Bioderma Tech. Co LTD;

[0079] - quaternary ammonium salts such as Methyl Niacinamide Chloride (name INCI) such as the commercial product MNA Chloride® from Pharmena;

[0080] - reaction products of niacinamide with polypeptides such as those derived yeast: INCI name Niacinamide / Yeast Polypeptide as the commercial product sold under the name Vitazyme B3® by Arch Personal Care Products, LP / Lonza Personal Care.

[0081] Niacinamide will be used more particularly, such as the commercial products sold under the name Niacinamide PC® (DSM Nutritional Products, Inc.), OriStar NA® (Orient Stars LLC), RonaCare Nicotinamide® (Merck KGaA / EMD Chemicals), RonaCare Nicotinamide® (EMD Chemicals).

[0082] In yet another embodiment of the invention, the cosmetic actives / formulas may also include panthenol. Panthenol is an analogue of pantothenic acid (vitamin B3).

[0083] (Peptides)

[0084] In exemplary embodiments, the anti-aging peptides provide long-term biological efficacy on wrinkles or lines. The anti-aging peptides may include, but are not limited to, one or more acetyl hexapeptides, acetyl hexapeptide-8, one or more acetyl tetrapeptides, acetyl tetrapeptide-9, PRPs (platelet-rich plasma peptides), and combinations thereof.

[0085] In other embodiments, the cosmetic actives / formulas may include one or more of acetyl hexapeptides (e.g., acetyl hexapeptide-3, -37, -38, and -51) and other acetyl tetrapeptides (e.g., acetyl tetrapeptide-2, -3, -5, -7, -8, -9, -11, and -15), and combinations thereof.

[0086] (Ceramides)

[0087] Ceramides are a family of waxy lipid molecules that are composed of sphingosine and a fatty acid. Ceramides include or may be selected from ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide IA, ceramide 611, ceramide AP, ceramide EOP, ceramide EOS, ceramide NP, ceramide NG, ceramide NS, ceramide AS, ceramide NS dilaurate, and a mixture thereof. In some cases, the ceramides include or may be selected from ceramide-EOS, ceramide-NS, ceramide-NP, ceramide-EOH, ceramide-AS, ceramide-NH, ceramide-AP, ceramide-AH, ceramide-OS, ceramide-OH, and a mixture thereof. In some cases, the cosmetic actives / formulas may include ceramide EOP, optionally, in combination with one, two or more other ceramides.For example, in some cases, cosmetic actives / formulas include a combination of ceramides, e.g., a combination of ceramide EOP, ceramide NP, ceramide AP.

[0088] (Growth factors)

[0089] Growth factors are typically peptides with diverse biological effects. Some families of growth factors that have been identified as useful in wound healing and / or epidermal remodeling include, for example, transforming growth factor-[3 (TGF-[3), epidermal growth factor (EGF), insulin-like growth factors (IGF), growth factor platelet-derived growth factors (PDGF) and fibroblast growth factors (FGF).

[0090] Each of the skin care actives may be provided in the cosmetic actives / formulas in any suitable amount. In exemplary embodiments, the amount of each skin care active may be between 0.001% and 30%, preferably between 0.01% and 20%, and most preferably between 0.1% and 10% based on the weight of the medium.

[0091] (Active ingredients)

[0092] In another embodiment of the invention, the cosmetic actives / formulas may include active ingredients, which are distinct from the skin care actives mentioned above. Non-limiting examples of the one or more active agents include adenosine, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), hyaluronic acid, lanolin, citric acid, malic acid, lactic acid, tartaric acid, salicylic acid, vitamin C, a vitamin, a retinoid, retinal, retinoic acid, a carotenoid, an amino acid, a protein, an enzyme and a coenzyme. In a number of cases, cosmetic actives / formulas include a humectant and moisturizing ingredients, an anti-aging agent, a depigmenting agent, an anti-wrinkle active, an agent that treats oily skin, and / or a skin penetration enhancer.

[0093] In another embodiment of the invention, the active ingredients / cosmetic formulas may include vitamin C and its derivatives and in particular vitamin CG, CP and vitamin C 3-0 ethyl, alpha and beta arbutin, ferulic acid, lucinol and its derivatives, kojic acid, resorcinol and its derivatives, tranexamic acid and its derivatives, gentisic acid, homogentisic acid, methyl gentisate or homogentisate, dioic acid, D-panthenate calcium sulfonate, lipoic acid, ellagic acid, vitamin B3, linoleic acid and its derivatives, ceramides and their homologues, derived from plants such as chamomile, bearberry, the aloe family (vera, ferox, barbadensis), mulberry, skullcap, kiwi water (Actinidia chinensis) marketed by Gattefosse, an extract of Paeonia suffruticosa root, such as that sold by Ichimaru Pharcos under the name Liquid Botanpi Be,a brown sugar extract (Saccharum offici narum) such as the molasses extract marketed by Taiyo Kagaku under the name Liquid Molasses, but this list is not exhaustive. Particular depigmenting agents include vitamin C and its derivatives, including vitamin CG, CP and vitamin C 3-0 ethyl, alpha and beta arbutin, ferulic acid, kojic acid, resorcinol and its derivatives, D-panthenol calcium sulfonate, lipoic acid, ellagic acid, vitamin B3, a kiwi fruit water (Actinidia chinensis) marketed by Gattefosse, and a Paeonia suffruticosa root extract, such as that sold by Ichimaru Pharcos under the name Botanpi Liquid B.

[0094] The expression "anti-wrinkle active" means a natural or synthetic compound producing a biological effect, such as increasing the synthesis and / or activity of certain enzymes, when brought into contact with an area of ​​wrinkled skin, which has the effect of reducing the appearance of wrinkles and / or fine lines. Exemplary anti-wrinkle actives may be chosen from: desquamating agents, anti-glycation agents, NO-synthase inhibitors, agents stimulating the synthesis of dermal or epidermal macromolecules and / or preventing their degradation, agents stimulating the proliferation of fibroblasts and / or keratinocytes, or stimulating agents for reducing the differentiation of keratinocytes; muscle relaxants and / or dermo-decontracting agents, and mixtures thereof.

[0095] Examples of these compounds are: adenosine and its derivatives, ascorbic acid and its derivatives such as magnesium ascorbyl phosphate and ascorbyl glucoside; tocopherol and its derivatives such as tocopherol acetate, nicotinic acid and its precursors such as nicotinamide; ubiquinone; glutathione and its precursors such as L-2-oxothiazolidine-4-carboxylic acid, C-glycoside compounds and their derivatives as described in particular in document EP-1 345 919, in particular C-beta-D-xylopyranoside-2-hydroxy-propane as described in particular in document EP-1 345 919, plant extracts including sea fennel and olive leaf extracts, as well as their plants and their hydrolysates such as rice protein hydrolysates or soy proteins;algae extracts, including kelp extracts, bacterial extracts, sapogenins such as diosgenin, and Dioscorea plant extracts, including wild yam, including: α-hydroxy acids, 3-hydroxy acids, such as salicylic acid, and n-octanoyl-5-salicylic oligopeptides and pseudo-dipeptides and their acylated derivatives, including {2-[acetyl-(3-trifluoromethyl-phenyl)-amino]-3-methyl-]acetic acid and lipopeptides marketed by the company under the brand names SEDERMA Matrixyl 500 and Matrixyl 3000; lycopene, manganese salts and magnesium salts, including gluconates, and mixtures thereof.

[0096] Among the adenosine derivatives, non-phosphate derivatives of adenosine are included, such as in particular 2'-deoxyadenosine, 2',3'-adenosine isopro-polylidene; toyocamycin, 1-methyladenosine, N-6-methyladenosine; adenosine N-oxide, 6-methylmercaptopurine riboside, and 6-chloropurine riboside.

[0097] Other derivatives include adenosine receptor agonists such as phenylisopropyl adenosine ("PIA"), 1-methylisoguanosine, N6-cyclohexyladenosine (CHA), N6-cyclopentyladenosine (CPA), 2-chloro-N6-cyclopentyladenosine, 2-chloroadenosine, N6-phenyladenosine, 2-phenylaminoadenosine, MECA, N6-phenethyladenosine, 2-p(2-carboxy-ethyl)phenethyl-amino-5'-N-ethylcarboxamido-adenosine (CGS-21680), N-ethylcarboxamido-adenosine (NECA), 5'-(N-cyclopropyl)-carboxamidoadenosine, DPMA (PD129.944) and metrifudil.

[0098] In exemplary embodiments, the skin penetration enhancers assist in the penetration of the cosmetic actives / formulas into the skin. The skin penetration enhancers may include, but are not limited to, oleic acid.

[0099] Each of the active ingredients may be provided in the cosmetic actives / formulas in any suitable amount. In exemplary embodiments, the amount of each active ingredient may be between 0.01% and 0.5% based on the weight of the medium.

[0100] (Additives)

[0101] In another embodiment of the invention, the cosmetic actives / formulas may include additives, which are distinct from the above-mentioned actives and skin care active ingredients. The additive may include, but is not limited to, at least one active, at least one pearlescent pigment, at least one polymer, at least one preservative, at least one surfactant, at least one non-aqueous solvent, or combinations thereof.

[0102] Suitable actives for cosmetic actives / formulas may include, but are not limited to, allantoin, bifid ferment lysate, calcium lactate, capryloyl salicylic acid, dipotassium glycyrrhizate, disodium ethylenediaminetetraacetic acid (EDTA), glycolic acid, potassium hydroxide, sodium hydroxide, trisodium ethylenediamine disuccinate, or combinations thereof.

[0103] Suitable pearlescent pigments for cosmetic actives / formulas may include, but are not limited to, composite particles including a substrate formed from mica, synthetic fluorphlogopite coated with at least one mineral pigment and at least one pearlescent pigment, selected from composite particles including at least one carrier selected from mica, synthetic fluorphlogopite or calcium and sodium borosilicate, and fully or partially coated with one or more layers of metal oxides, in particular selected from titanium dioxide, iron oxide, tin oxide, or combinations thereof.

[0104] Suitable polymers for cosmetic actives / formulas may include, but are not limited to, ammonium polyacryloyldimethyl taurate, carrageenan (and) potassium chloride, ceratonia siliquia (locust bean) gum (and) sucrose, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, polyacrylamide (and) C13-C14 isoparaffin (and) Laureth-7, xanthan gum, or combinations thereof.

[0105] Suitable preservatives for cosmetic actives / formulas may include, but are not limited to, chlorphenesin, phenoxyethanol, sodium benzoate, or combinations thereof.

[0106] Suitable surfactants for cosmetic actives / formulas may include, but not limited to, glyceryl stearate, myristic acid, palmitic acid, stearic acid, polyethylene glycol (PEG)-100 stearate, PEG-60 hydrogenated castor oil, steareth-100, or combinations thereof.

[0107] Suitable non-aqueous solvents for cosmetic actives / formulas may include, but are not limited to, denatured alcohol, caprylyl glycol, glycerin, 1,2-hexanediol, methylpropanediol, or combinations thereof.

[0108] In a further embodiment of the invention, the cosmetic actives / formulas may include any number of other suitable additives or adjuvants. Representative additives and adjuvants include, for example, water-soluble or water-miscible solvents or co-solvents, dispersion enhancers, colorants, fillers, antioxidants (e.g., EDTA, butylated hydroxytoluene (BHT), and tocopherol), essential oils, fragrances, tints, neutralizing or pH-adjusting agents (e.g., citric acid, trimethylamine (TEA), and sodium hydroxide), conditioning or softening agents (e.g., panthenol and allantoin), and extracts, such as botanical extracts.Examples of anti-aging active agents or dermatological active agents include sunscreen agents (e.g., inorganic sunscreen agents, such as titanium dioxide and zinc oxide, and organic sunscreen agents, such as octocrylene, ethylhexyl methoxycinnamate, and avobenzone), keratolytic agents, anti-elastase and anti-ollagenase agents, fatty acid derivatives, steroids, trace elements, plankton extracts, enzymes and coenzymes, flavonoids, hydroxy acids and mixtures thereof, and facilitating agents.

[0109] Each of the additives may be provided in the cosmetic actives / formulas in any suitable amount. The total amount of the at least one additive may be any suitable amount, such as, for example, about 50% or less, alternatively from about 20% to about 50%, alternatively from about 25% to about 45%, alternatively from about 25% to about 40%, alternatively from about 30% to about 35%, or any suitable value, range or sub-range thereof, by weight, based on the weight of the medium. Thus, the additives may be present in a total amount of about 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, to about 50%.

[0110] (Others)

[0111] In one embodiment, the cosmetic actives / formulas may include an exfoliation enhancer composition, an anti-aging enhancer composition, an enhancer additive, a base composition, an aqueous alcohol base composition, an aqueous emulsion base, and a composition.

[0112] To date, techniques have been developed for applying ultrasonic waves to the skin surface. For example, WO 2019 / 164836 A1 discloses a technology that combines HIFU and cellulite treatment agents. WO 2019 / 164836 A1 performs cellulite treatment by injecting the agents into the dermis of the skin using a needle syringe after the application of the ultrasonic waves. In contrast, the method of said one embodiment of the invention simply applies the ultrasound transmission medium to the skin surface before the application of ultrasonic waves without using a highly invasive method such as using a needle syringe. Therefore, the method of said one embodiment of the present invention is less invasive than the aforementioned conventional technique.Furthermore, since the procedure is less invasive, it can be performed without a high degree of specialization, making it suitable for home or salon practice.

[0113] <Kit pour la mise en œuvre du procédé>

[0114] A kit for carrying out the method of an embodiment of the invention will be described. It should be noted that a kit for carrying out the method of an embodiment of the invention is not limited to the following kit. Skin wrinkles are generally divided into epidermal wrinkles and dermal wrinkles. Epidermal wrinkles are characterized by their shallowness and fineness, and are also called superficial wrinkles. Epidermal wrinkles are said to be caused by a lack of water in the stratum corneum of the epidermis. On the other hand, dermal wrinkles are said to be caused by the loss of elasticity of the skin. The loss of elasticity of the skin is said to be caused by a decrease or degeneration of collagen and elastin in the dermis of the skin due to damage caused by ultraviolet rays and aging, etc.

[0115] Professional HIFU has been used to improve these wrinkles. HIFU devices focus high-energy ultrasound waves onto the skin. However, HIFU devices can be invasive due to their nature of delivering high-energy ultrasound into the skin.

[0116] On the other hand, the kit used in the method of one embodiment of the invention combines ultrasonic waves with a medium including cosmetic actives / formulas to be used for a cosmetic benefit and it can also be used to smooth skin wrinkles in a less invasive manner.

[0117] Hereinafter, a kit according to one embodiment of the invention will be described with reference to the drawings. [Fig. 2] is a schematic view showing the configuration of a kit according to one embodiment of the invention. As shown in [Fig. 2], the kit 20 comprises a cosmetic device 10 and a medium 6 including cosmetic actives / formulas. In another embodiment of the invention, the device is not born necessarily limited to cosmetic use. In another embodiment of the invention, the device is of the portable type.

[0118] As shown in [Fig. 2], the cosmetic device 10 comprises an electronic unit 2, a transducer 3, a focusing unit 4, a housing 1 and a head 5. In one embodiment of the invention, an ultrasonic wave unit 7 is composed of the transducer 3 and the focusing unit 4. In another embodiment of the invention, the ultrasonic unit includes only a transducer. In another embodiment of the invention, the focused ultrasonic waves can be produced without a focusing unit, but with the transducer alone.

[0119] In another embodiment of the invention, the cosmetic device includes a transducer, a focusing unit, a housing and a head.

[0120] In one embodiment of the invention, the electronic unit 2 comprises a control unit and a command unit (not shown).

[0121] The control unit is an interface for synchronizing a selection of operating parameters such as the frequency of the ultrasonic waves, the energy of the ultrasonic waves and / or the operating mode. In one embodiment of the invention, the control unit is used to control the drive unit according to the operating parameters.

[0122] The drive unit controlled by the control unit drives the transducer 3 and the focusing unit 4 of the ultrasonic wave unit 7.

[0123] In another embodiment of the invention, the electronic unit may comprise a rechargeable battery unit. When the electronic unit comprises a rechargeable battery unit, the kit according to one embodiment of the invention can be used even in a place without a power source (e.g., outdoors).

[0124] In one embodiment of the invention, the transducer 3 emits ultrasonic waves that are focused and pulsed ultrasonic waves and have a frequency of 10 kHz to 50 MHz. As described above, the ultrasonic waves are generated such that the ultrasonic waves propagate into the skin to a maximum depth of 3 mm from the surface of the skin and generate a rise in skin temperature at the application areas. The transducer 3 is not particularly limited in the extent to which it can emit ultrasonic waves. The transducer 3 may be a single-point fixed piezoelectric transducer. When the transducer of the cosmetic device of the kit is a single-point fixed piezoelectric transducer, the kit can be suitably used for smaller application areas, such as wrinkles at the corners of the eyes.In another embodiment of the invention, the transducer is a single linearly moving piezoelectric transducer. In another embodiment of the invention, the device may include an actuator. The actuator may linearly move the piezoelectric transducer. single in a length range of 10.0 mm to 100.0 mm, and preferably 10.0 mm to 50.0 mm. The kit with the single transducer may also be suitable for treating larger areas such as the cheek, forehead, full face and neck.

[0125] In another embodiment of the invention, the transducer is comprised of an array of a plurality of fixed piezoelectric transducers. The fixed plurality of piezoelectric transducers may be arranged substantially parallel to the head of the device. When the transducer of the cosmetic device of the kit is an array of a plurality of fixed piezoelectric transducers, the kit may be suitably used for larger application areas such as the cheeks. In another embodiment of the invention, the transducer is a single point fixed piezoelectric transducer, a single linear fixed piezoelectric transducer, an array of a plurality of fixed piezoelectric transducers, and / or a single linear displacement piezoelectric transducer. For example, the type of transducer may be selected based on the shape and / or size of the areas to which the kit is applied.

[0126] In yet another embodiment of the invention, the transducer may be comprised of an array of at least 20 piezoelectric transducers. In yet another embodiment of the invention, a plurality of transducers may be arranged in a 1D (i.e., linear) shape or any 2D (i.e., matrix) shape.

[0127] In one embodiment of the invention, the focusing unit 4 adjusts a depth of the ultrasonic waves.

[0128] The ultrasonic waves can increase and / or enhance an effect of the active ingredients / cosmetic formulas included in the medium 6 for the skin. Therefore, the kit according to one embodiment of the invention should provide perceptible cosmetic benefits for the skin.

[0129] HIFU has been mainly applied to a flat or striped localized region of the skin. In contrast, in one embodiment of the invention, the focusing unit 4 can focus the ultrasound waves onto a point-shaped, linear or 3D region. Therefore, the kit according to one embodiment of the invention is also effective for relatively small areas and is effective in improving wrinkles of any shape and / or size in the skin.

[0130] In one embodiment of the invention, the housing 1 houses the electronic unit 2 and the transducer 3 and the focusing unit 4 of the ultrasonic wave unit 7.

[0131] In another embodiment of the invention, the housing houses the transducer and the focusing unit of the ultrasonic wave unit.

[0132] In one embodiment of the invention, the head 5 is close to or in contact with the skin. During use of the kit 20, the ultrasonic waves propagate from the head 5 to the skin via the medium 6. The ultrasonic waves can increase and / or enhance the effect of cosmetic active ingredients / formulas for the region located between the stratum corneum and the epidermal layer of the skin.

[0133] In another embodiment of the invention, the head is part of a cartridge attached to the housing. The cartridge may be removable and / or replaceable. In this embodiment, the cartridge may be replaced with a cartridge having a desired head depending on the applied location and use of the kit. In this embodiment, when the head is dirty, only the cartridge may be removed to clean the cartridge head. In this embodiment, when the head is damaged, the cartridge may be replaced with a new cartridge.

[0134] In one embodiment of the invention, the medium 6 is a cosmetic material that can be applied to the surface of the skin. The medium 6 is also a material for perceiving the cosmetic benefits of the skin and propagating the ultrasonic waves. In addition, the skin is gently warmed by the ultrasonic waves at the focal point in a short time. When the skin is warmed, the effect of the active ingredients / cosmetic formulas included in the medium 6 for the skin can be increased and / or improved.

[0135] In one embodiment of the invention, the medium 6 is a composition including cosmetic active ingredients / formulas. The cosmetic active ingredients / formulas may include the ingredients described above.

[0136] HIFU devices known for professionals are invasive because they use high-energy ultrasound waves. Therefore, HIFU devices can only be used by experts or dermatologists and are considered a form of medical treatment or equivalent.

[0137] On the other hand, the kit according to one embodiment of the invention uses ultrasonic waves of lower energy than HIFU devices and, therefore, the surface temperature of the applied areas of the skin is lower than that of HIFU devices, so that the kit is less invasive. Therefore, the kit according to one embodiment of the invention does not require a medical license for use and is intended for non-medical treatment. In other words, the kit according to one embodiment of the invention is intended for use in salons or at home.

[0138] In another embodiment of the invention, the electronic unit may further include a temperature sensor and a cooling unit. The temperature sensor detects whether the temperature of the medium applied to the skin to deliver the ultrasonic waves to the skin is outside a predetermined temperature range. The cooling unit cools the medium if necessary. When the control unit determines that the temperature of the medium becomes higher than the temperature range predetermined following detection by the temperature sensor, the control unit can control the driving unit so that the cooling unit is driven to cool the medium. Therefore, the kit according to one embodiment of the invention has less thermal effect on the skin and is less invasive.

[0139] <Méthode d’utilisation d’un kit>

[0140] A method of using the kit according to one embodiment of the invention will be described. The method of using the kit according to one embodiment of the invention is a cosmetic method. In other words, the method of using the kit according to one embodiment of the invention is a non-therapeutic method. [Fig. 3] is a block diagram showing the method of using the kit according to one embodiment of the invention. In the method 200 shown in [Fig. 3], the kit 20 shown in [Fig. 2] is used. The method 200 includes a block 210, a block 220, a block 230, a block 240, a block 250 and a block 260.

[0141] In one embodiment of the invention, in block 210, the step of applying the medium 6 of [Fig.2] to the surface of the skin is carried out.

[0142] In one embodiment of the invention, in block 220, the step of controlling the control unit according to the operating parameters using the control unit of the electronic unit 2 of [Fig.2] is carried out.

[0143] In one embodiment of the invention, in block 230, the step of driving the transducer 3 and the focusing unit 4 of the ultrasonic wave unit 7 of [Fig.2] by means of the controlled driving unit is carried out.

[0144] In one embodiment of the invention, at block 240, the step of generating the ultrasonic waves by means of the ultrasonic wave unit is performed. In one embodiment of the invention, the frequency and energy of the ultrasonic waves may be used to indirectly refer to the depth of the ultrasonic waves relative to the skin surface.

[0145] In one embodiment of the invention, the ultrasonic waves have a frequency of 10 kHz to 50 MHz, preferably 30 kHz to 20 MHz, more preferably 500 kHz to 10 MHz, and more preferably 1 MHz to 10 MHz. The ultrasonic waves may have a frequency of 3 MHz to 9 MHz and may have a frequency of 5 MHz to 9 MHz.

[0146] In one embodiment of the invention, the ultrasonic waves have an energy equal to or greater than 0.001 J and less than 10 J, preferably equal to or greater than 0.01 J and less than 5 J.

[0147] In one embodiment of the invention, the temperature of the skin at the focal zone when applying the ultrasonic waves to the skin is greater than 40°C and less than or equal to 65°C, preferably greater than 40°C and less than or equal to 60°C, and more preferably greater than 40°C and less than or equal to 50°C.

[0148] In one embodiment of the invention, at block 250, the step of bringing the head 5 of [Fig.2] into contact with the skin or the medium 6 on the skin is carried out.

[0149] In one embodiment of the invention, at block 260, the step of propagating the ultrasonic waves through the medium 6 to the skin is carried out.

[0150] Thus, one embodiment of the invention may relate to the method combining the medium including cosmetic active ingredients / formulas and the ultrasound waves for facial enhancement, which are of lower energy than HIFU, the kit for implementing the method, and a method for using the kit.

[0151] In one embodiment of the invention, before the step of generating the ultrasonic waves, the step of applying the medium 6 to the surface of the skin is carried out, without being limited thereto. The step of applying the medium 6 to the surface of the skin can be carried out at any time before the step of propagating the ultrasonic waves through the medium 6 to the skin.

[0152] In another embodiment of the invention, when the ultrasonic wave unit comprises only the transducer, known focusing methods are used to focus the ultrasonic waves.

[0153] In yet another embodiment of the invention, the cosmetic device of the kit does not necessarily comprise a control unit and a driving unit. Therefore, the method of yet another embodiment of the invention does not include the steps of controlling the driving unit and driving the transducer.

[0154] Although an example of the present invention has been presented with reference to the drawings, the present invention is not limited to the drawings. In view of the above teachings, numerous modifications and variations of the present invention are possible. Therefore, within the scope of the claims, the claimed invention may be practiced by methods other than those specifically described in this specification. Examples

[0155] Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to these examples. Example 1

[0156] A hyaluronic acid serum (manufactured in-house by L'Oréal) was applied to half of the subject's face as a medium. The molecular weight of hyaluronic acid in the hyaluronic acid serum was 50 kJ at 1.2 MDa. The concentration of hyaluronic acid was 1.5% relative to the total mass of the hyaluronic acid serum. The dynamic viscosity of the hyaluronic acid serum was 3.5 steps.

[0157] After application, a kit shown in [Fig.2], comprising a piezoelectric transducer array, was applied to the skin under the following conditions, and these tests were carried out 5 times in a row every other day. The experts visually observed the wrinkles under the eyes and the wrinkles at the corners of the eyes (also called "crow's feet") of the subject.

[0158] Focusing depth of focused ultrasonic waves: 2.5 mm.

[0159] Frequency of focused ultrasonic waves: 7.5 MHz

[0160] In Example 1, it was confirmed that the wrinkles under and at the corners of the subject's eyes were improved after one week from the last trial.

[0161] <Exemple 2>

[0162] The test was carried out in the same manner as in Example 1, except that the subject's nasolabial lines were observed further after each test.

[0163] <Exemple comparatif 2>

[0164] The test was carried out in the same manner as in Example 2, except that the ultrasonic waves were not applied to the skin after application.

[0165] In Example 2, the effectiveness against wrinkles under the eyes was confirmed immediately after each trial. In addition, the wrinkles under and at the corners of the eyes, and the nasolabial lines of the subject after one week from the last trial were confirmed to be cumulatively improved by continuous trials. Therefore, the method of one embodiment of the invention has been shown to provide immediate and / or cumulative improvements in wrinkles under and at the corners of the eyes, and nasolabial lines.

[0166] On the other hand, the result of comparative example 2 showed that the effectiveness against wrinkles under and at the corner of the eyes, and nasolabial lines was lower than that of example 2 immediately after each test.

[0167] From the above results, it has been shown that the present invention is useful. Legend

[0168] 1 Box

[0169] 2 Electronic unit

[0170] 3 Transducer

[0171] 4 Focusing unit

[0172] 5 Head

[0173] 6 Middle

[0174] 7 Ultrasonic wave unit

[0175] 10 Cosmetic device

[0176] 20 Kit

[0177] 100 Method for reducing wrinkles on a skin surface

[0178] 110 Block

[0179] 120 Block

[0180] 200 Method of using a kit

[0181] 210 Block

[0182] 220 Block

[0183] 230 Block

[0184] 240 Block

[0185] 250 Block

[0186] 260 Block

Claims

Claims

1. A non-therapeutic cosmetic method for reducing wrinkles on a skin surface, the method comprising the steps of: applying an ultrasound transmitting medium to the skin surface to be treated, wherein the medium includes cosmetic actives / formulas and wherein the cosmetic actives / formulas include hyaluronic acid and applying ultrasonic waves to the skin surface with the applied medium, wherein the ultrasonic waves produce an acoustic output, wherein the ultrasonic waves have a frequency of 1 MHz to 10 MHz, and wherein the ultrasonic waves are pulsed and focused ultrasonic waves and wherein the pulse energy of the ultrasonic waves is 0.01 J or more and less than 5 J,wherein the ultrasonic waves are generated in such a way that the ultrasonic waves propagate into the skin to a maximum depth of 3 mm from the skin surface and generate a rise in skin temperature at the application areas.,

2. The method of claim 1, wherein the pulse duration of the ultrasonic waves is 4 ms to 50 ms.

3. The method of claim 1, wherein the peak power of the ultrasonic waves is less than 80 W, preferably 65 W or less, more preferably 15 W or less, even more preferably 5 W or less, and even more preferably 0.1 W to 2 W.

4. The method of claim 1, wherein the ultrasonic waves are propagated to one or more layers of the skin, which are the epidermis and / or the dermis.

5. The method of claim 1, wherein a concentration of the hyaluronic acid is greater than 0.1% relative to the total mass of the medium.

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