Device, resistance measuring method, cell fusion method and electroporation method

Abstract

To provide a device that can be used in any of resistance measuring devices, cell fusion devices, and electroporation devices, and has a simple configuration and is highly safe.SOLUTION: Provided is a device 1 for applying a voltage or a current to a sample, including an electrode portion 2 and a lid portion 3 formed of a non-conductive material. The electrode portion includes a first substrate 21 formed of a non-conductive material, a first electrode, and a second electrode. The first electrode and the second electrode are fixed to the first substrate so as not to be in contact with each other. The device 1 includes: a shielding portion 32 for preventing a user from touching the first electrode and / or the second electrode; a first terminal insertion hole 33 for making a first terminal 41 for being connected to a power source contact with the first electrode through a lid portion in a state that the electrode portion and the lid portion are engaged with each other; and a second terminal insertion hole 34 for making a second terminal 42 for being connected to the power source contact with the second electrode through the lid portion in a state that the electrode portion and the lid portion are engaged with each other.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The disclosure in the present application relates to a device used as a resistance measuring device, a cell fusion device, or an electroporation device (hereinafter, may be simply described as a "device"), and a resistance measuring method, a cell fusion method, and an electroporation method.

Background Art

[0002] Devices for applying a voltage to a sample are known. As an example of a device, Patent Document 1 discloses that a container and a lid are formed by transparent resin molding, a tank is provided in the container, the tanks are adjacent to each other in a direction orthogonal to the width direction via a breakable partition wall having the same width, and a cell electroprocessing chamber capable of breaking the partition wall to make a plurality of tanks into a series is disclosed.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] The cell electroprocessing chamber described in Patent Document 1 is an invention that aims to enable use in both applications of treating a small amount of cell suspension and a large amount of cell suspension with one type of cell electroprocessing chamber. In the invention described in Patent Document 1, the tanks provided in the container are adjacent to each other in a direction orthogonal to the width direction via a breakable partition wall having the same width, and are formed so that the partition wall can be broken to make a plurality of tanks into a series, and the electrodes are arranged on the lid side rather than on the container side provided with the tanks, thereby solving the problem.

[0005] By the way, as a device for applying voltage or current to a sample, a resistance measuring device for a liquid sample, a cell fusion device, or an electroporation device is known. However, the invention described in Patent Document 1 is a device specialized for cell fusion or electroporation. There is no known device that can be used in any of a resistance measuring device, a cell fusion device, and an electroporation device, has a simple configuration, and has high safety.

[0006] The disclosure of the present application has been made to solve the above problems. That is, the object of the disclosure of the present application is to provide a device that can be used in any of a resistance measuring device, a cell fusion device, and an electroporation device, has a simple configuration, and has high safety.

Means for Solving the Problems

[0007] The disclosure in the present application relates to the following device, resistance measuring method, cell fusion method, and electroporation method.

[0008] (1) A device for applying voltage or current to a sample, The device includes an electrode part and a lid part formed of a non-conductive material, The electrode part, A first base material formed of a non-conductive material, A first electrode, A second electrode, And includes, One end of the first electrode is fixed to the first base material, and the other end of the first electrode has a first protruding end protruding from the first base material, One end of the second electrode is fixed to the first base material, and the other end of the second electrode has a second protruding end protruding from the first base material, The first electrode and the second electrode are fixed to the first base material so as not to be in contact with each other, When the lid part is fitted to the electrode part from the side of the first protruding end and the second protruding end, A second base material that covers the first protruding end and the second protruding end, A shielding portion protruding from the second base material to prevent a user from contacting the first electrode and / or the second electrode; A first terminal insertion hole for bringing a first terminal for connecting to a power source into contact with the first electrode through the lid portion in a state where the electrode portion and the lid portion are fitted; A second terminal insertion hole for bringing a second terminal for connecting to a power source into contact with the second electrode through the lid portion in a state where the electrode portion and the lid portion are fitted; and a device. (2) The sample is a liquid sample, and the device is used as a resistance measuring device for measuring the resistance of the liquid sample The device according to (1) above. (3) The sample contains cells, and the device is used as a cell fusion device or an electroporation device The device according to (1) above. (4) The first electrode is formed in an endless shape except for the one end and the first protruding end, The second electrode is formed in an endless shape except for the one end and the second protruding end, In the first electrode and the second electrode fixed to the first base material, the electrode fixed to the outside is defined as the first electrode, and the electrode fixed to the inside of the first electrode is defined as the second electrode. The space formed between the first electrode and the second electrode is defined as a sample accommodation portion, The surface of the first electrode that forms the sample accommodation portion is defined as the inner surface of the first electrode, and the surface opposite to the surface of the first electrode that forms the sample accommodation portion is defined as the outer surface of the first electrode. When the surface of the second electrode that forms the sample accommodation portion is defined as the inner surface of the second electrode, and the surface opposite to the surface of the second electrode that forms the sample accommodation portion is defined as the outer surface of the second electrode, the shielding portion is formed to protrude from the second base material and includes a first shielding portion for preventing a user from contacting the outer surface of the first electrode. The device according to (1) above. (5) The shielding portion is formed to protrude from the second base material, and further includes a second shielding portion for preventing a user from contacting the outer surface of the second electrode. The device according to (4) above. (6) When the first electrode and the second electrode are viewed from the first protruding end and the second protruding end sides, the first electrode and the second electrode are formed in a substantially circular shape with different diameters. The first shielding portion is formed in a substantially circular shape having an inner shape larger than the outer shape of the first electrode. The device according to (4) above. (7) When the first electrode and the second electrode are viewed from the first protruding end and the second protruding end sides, the first electrode and the second electrode are formed in a non-circular similar shape. The inner shape of the first shielding portion is similar to the outer shape of the first electrode, and the inner shape is larger than the outer shape of the first electrode. The device according to (4) above. (8) The first electrode and the second electrode are substantially plate-shaped and are fixed to the first base material so as to be substantially parallel. A space formed between the first electrode and the second electrode is defined as a sample accommodation portion. A surface of the first electrode that forms the sample accommodation portion is defined as the inner surface of the first electrode, and a surface opposite to the surface of the first electrode that forms the sample accommodation portion is defined as the outer surface of the first electrode. When a surface of the second electrode that forms the sample accommodation portion is defined as the inner surface of the second electrode, and a surface opposite to the surface of the second electrode that forms the sample accommodation portion is defined as the outer surface of the second electrode, The shielding portion is formed to protrude from the second base material and is formed to prevent a user from contacting the outer surfaces of the first electrode and the second electrode. The device according to (1) above. (9) A first recess for accommodating a part of the first terminal inserted through the first terminal insertion hole is formed on the outer surface of the first electrode. A second recess for accommodating a part of the second terminal inserted through the second terminal insertion hole is formed on the outer surface of the second electrode. The device according to any one of (4) to (8) above. (10) A method for measuring the resistance of a liquid sample using the device according to any one of (1) to (2), (4) to (8) above, wherein the resistance measurement method comprises: A sample introduction step of introducing a liquid sample into a sample storage portion formed between a first electrode and a second electrode of an electrode portion; A lid fitting step of fitting a lid portion to the electrode portion; With the lid portion fitted to the electrode portion, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage or current application step of applying a voltage or current to the first electrode and the second electrode; A resistance measurement step of measuring the resistance of the liquid sample; Including A method for measuring the resistance of a liquid sample. (11) A cell fusion method using the device according to any one of (1), (3) to (8) above, wherein the cell fusion method comprises: A sample introduction step of introducing a sample solution containing cells into a sample storage portion formed between a first electrode and a second electrode of an electrode portion; A lid fitting step of fitting a lid portion to the electrode portion; With the lid portion fitted to the electrode portion, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage application step of applying a voltage to the first electrode and the second electrode; A cell fusion step of fusing cells by applying a voltage; Including A cell fusion method. An electroporation method using the device according to any one of (1), (3) to (8) above, wherein the electroporation method comprises: A sample introduction step of introducing a sample solution containing cells and an introduction substance into a sample accommodation part formed between a first electrode and a second electrode of an electrode part; A lid fitting step of fitting a lid part to the electrode part; With the lid part fitted to the electrode part, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage application step of applying a voltage to the first electrode and the second electrode; A substance introduction step of introducing an introduction substance into cells by applying a voltage; and comprising an electroporation method.

Advantages of the Invention

[0009] The device disclosed in the present application can be used for any of a resistance measurement device, a cell fusion device, and an electroporation device. Further, the device disclosed in the present application employs a structure in which, after fitting a lid part to an electrode part having two electrodes forming a sample accommodation part fixed thereto, terminals for connection to a power source are inserted into terminal insertion holes provided in the lid part and brought into contact with the electrodes of the electrode part. That is, the lid part is used to function as a positioning function for the terminals and a shielding function for the electrodes. Therefore, by adopting a configuration in which it is difficult to apply a voltage or current between the electrodes without the lid, a device with a simple configuration and high safety can be provided.

Brief Description of the Drawings

[0010]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Embodiments for Carrying Out the Invention

[0011] Hereinafter, embodiments of the device will be described in detail with reference to the drawings. In this specification, members having the same kind of functions are denoted by the same or similar reference numerals. And, the repetitive description may be omitted for the members denoted by the same or similar reference numerals.

[0012] In addition, the positions, sizes, ranges, etc. of the respective components shown in the drawings may not represent the actual positions, sizes, ranges, etc. for the sake of easy understanding. Therefore, the disclosure in this application is not necessarily limited to the positions, sizes, ranges, etc. disclosed in the drawings.

[0013] (Definition of direction) In this specification, the vertically downward direction is defined as the Z direction.

[0014] (Embodiment of Device 1) Referring to FIGS. 1 to 3, various embodiments of Device 1 will be described. FIG. 1A is a schematic top view of the electrode portion 2 constituting Device 1, and FIG. 1B is a cross-sectional view taken along the arrow in the X-X' direction of FIG. 1A. FIG. 2A is a schematic top view of the lid portion 3 constituting Device 1, and FIG. 2B is a cross-sectional view taken along the arrow in the X-X' direction of FIG. 2A. FIG. 3A is a cross-sectional view when the lid portion 3 shown in FIG. 2B is fitted to the electrode portion 2 shown in FIG. 1B, and FIG. 3B is a cross-sectional view showing a state in which the first terminal 41 and the second terminal 42 are further inserted into FIG. 3A.

[0015] Device 1 according to the embodiment includes an electrode portion 2 and a lid portion 3 formed of a non-conductive material. The electrode portion 2 includes a first base material 21 formed of a non-conductive material, a first electrode 22, and a second electrode 23.

[0016] One end of the first electrode 22 is fixed to the first base material 21, and the other end of the first electrode 22 has a first protruding end 22a protruding from the first base material 21. One end of the second electrode 23 is fixed to the first base material 21, and the other end of the second electrode 23 has a second protruding end 23a protruding from the first base material 21. The first electrode 22 and the second electrode 23 are fixed to the first base material 21 so as to be non-contact with each other.

[0017] The first substrate 21 is not particularly limited as long as it is a non-conductive material, and resins or the like known in the art may be used. When the device 1 is used as a cell fusion device or an electroporation device, the electrode portion 2 may be repeatedly subjected to high-temperature and high-pressure treatment (about 120°C, about 2 atm). Since it is preferable that it does not deform even when the high-temperature and high-pressure treatment is repeated and can be observed with a microscope, the first substrate 21 is preferably a transparent heat-resistant resin. Examples of resins having such characteristics include, but are not limited to, polymethylpentene (PMP), polyarylate (PAR), polyallylsulfone (PASF), polysulfone (PSU), polycarbonate (PC), and the like.

[0018] The first electrode 22 and the second electrode 23 are not particularly limited as long as they are conductive materials. There is no particular limitation as long as they are conductive materials generally used in the fields of cell fusion and electroporation. For example, iron, stainless steel, platinum, aluminum, gold, carbon, and the like can be mentioned.

[0019] In the embodiment of the device 1 shown in FIGS. 1 to 3, except for one end of the first electrode 22 and the first protruding end 22a, it is formed in an endless shape (substantially circular shape). Similarly, the second electrode 23 is formed in an endless shape (substantially circular shape) except for one end and the second protruding end 23a. Further, the second electrode 23 is formed in a similar shape smaller than the first electrode 22, and the second electrode 23 is disposed inside the first electrode 22. Therefore, in the device 1 shown in FIGS. 1 to 3, the space formed between the first electrode 22 and the second electrode 23 becomes the sample accommodation portion 24. In this specification, the surface of the first electrode 22 that forms the sample accommodation portion 24 is defined as the first electrode inner surface 2b, and the surface on the opposite side of the surface of the first electrode 22 that forms the sample accommodation portion 24 is defined as the first electrode outer surface 22c. Also, the surface of the second electrode 23 that forms the sample accommodation portion 24 is defined as the second electrode inner surface 23b, and the surface on the opposite side of the surface of the second electrode 23 that forms the sample accommodation portion 24 is defined as the second electrode outer surface 23c.

[0020] The first electrode 22 and the second electrode 23 need to be fixed to the first substrate 21 in a liquid-tight manner. When it is not necessary to perform high-temperature and high-pressure treatment on the electrode portion 2, as shown in FIG. 1, the first electrode 22 and the second electrode 23 may be adhered to the first substrate 21 using an adhesive or the like.

[0021] On the other hand, when the electrode portion 2 is repeatedly subjected to high-temperature and high-pressure treatment, if an adhesive is used, the adhesive may deteriorate, and gaps may occur in the portions where the first electrode 22 and / or the second electrode 23 are fixed to the first substrate 21. Therefore, when the electrode portion 2 is repeatedly subjected to high-temperature and high-pressure treatment, as shown in FIG. 3A, holes are provided in the first electrode 22 and the second electrode 23, and the first substrate 21 and the first electrode 22 and the second electrode 23 may be fixed using a fixing member 25 such as a screw. In the examples shown in FIGS. 1 and 3, one end of the first electrode 22 and the second electrode 23 is fixed so as to be in contact with the first substrate 21. Alternatively, one end of the first electrode 22 and / or the second electrode 23 may be embedded in the first substrate 21.

[0022] The distance w between the first electrode 22 and the second electrode 23, in other words, the distance between the inner surface 22b of the first electrode and the inner surface 23b of the second electrode, may be appropriately set according to the purpose. Although not limited, when the device 1 is used as a resistance measuring device, the distance w is preferably 0.1 mm to 100 mm, when used as a cell fusion device, the distance w is preferably 0.1 mm to 10 mm, and when used as an electroporation device, the distance w is preferably 0.1 mm to 10 mm. If the distance w between the first electrode 22 and the second electrode 23 varies depending on the location, the experimental conditions will be different. Therefore, the distance w between the first electrode 22 and the second electrode 23 is preferably the same length regardless of the location. That is, when viewed in the Z direction, the inner surface 22b of the first electrode and the inner surface 23b of the second electrode are preferably formed to be similar in shape.

[0023] The thickness L of the first electrode 22 is not particularly limited as long as it can be fixed to the first base material 21 and maintain its shape (the distance w between the first electrode 22 and the second electrode 23 does not change). Although not limited, a preferable example of the thickness L of the first electrode 22 is in the range of 1 mm to 20 mm. The thickness L of the second electrode 23 may be the same as that of the first electrode 23 in the range of 1 mm to 20 mm, or may be different from the thickness L of the first electrode 22 as long as its shape can be maintained.

[0024] When the lid portion 3 is fitted to the electrode portion 2 from the sides of the first protruding end 22a and the second protruding end 23a of the electrode portion 2, the lid portion 3 includes a second base material 31 that covers the first protruding end 22a and the second protruding end 23a, and a shielding portion 32 that protrudes from the second base material 31 to prevent the user from contacting the first electrode 22 and / or the second electrode 23. Further, the lid portion 3 includes a first terminal insertion hole 33 for bringing the first terminal 41 for connecting to the power source 4 into contact with the first electrode 22, and a second terminal insertion hole 34 for bringing the second terminal 42 for connecting to the power source 4 into contact with the second electrode 23, in a state where the electrode portion 2 and the lid portion 3 are fitted together.

[0025] The non-conductive material forming the lid portion 3 is not particularly limited as long as it is non-conductive, and a resin or the like known in the art may be used. As the material forming the lid portion 3, similar to the first base material 21, it is preferably not deformed even when repeated high-temperature and high-pressure treatments are performed and can be observed with a microscope. Although not limited, for example, polymethylpentene (PMP), polyarylate (PAR), polyallylsulfone (PASF), polysulfone (PSU), polycarbonate (PC), etc. may be mentioned.

[0026] The shielding portion 32 is not particularly limited as long as it can prevent the user from accidentally contacting the first electrode 22 and / or the second electrode 23 when applying a voltage or current to the first electrode 22 and the second electrode 23. In the example shown in FIG. 1, the first electrode 22 as viewed in the Z direction is formed in a substantially circular shape. Therefore, the shielding portion 32 shown in FIG. 2 is formed in a substantially circular shape having an inner shape 35 larger than the outer shape 26 of the first electrode 22 (in other words, the shape formed by the outer surface 22c of the first electrode as viewed in the Z direction. It is circular in the example shown in FIG. 1.), and shields the outer surface 22c of the first electrode. Therefore, it prevents the user from accidentally contacting the outer surface 22c of the first electrode. When it is described in this specification that "having an inner shape 35 larger than the outer shape 26 of the first electrode 22", when the lid portion 3 is fitted to the electrode portion 2, a large frictional force does not occur between the shielding portion 32 and the first electrode 22, and it means that the size is such that an unnecessary gap does not occur. Although not limited, when the lid portion 3 is fitted to the electrode portion 2, the gap between the outer surface 22c of the first electrode and the shielding portion 32 may be about 0.1 mm to 10 mm.

[0027] Also, in the examples shown in FIGS. 2 and 3, no holes are formed in the second base material 31 other than the first terminal insertion hole 33 and the second terminal insertion hole 34. Therefore, by the shielding portion 32 shielding the outer surface 22c of the first electrode, it is possible to prevent the user from contacting the second electrode 23. In this specification, the shielding portion that shields the outer surface 22c of the endless first electrode 22 may be described as the first shielding portion 32a.

[0028] The shielding portion 32 may be integrally formed with the second base material 31. Alternatively, the shielding portion 32 may be formed separately from the second base material 31 and fixed to the second base material 31 using an adhesive or a fixing member.

[0029] The location where the first terminal insertion hole 33 is formed may be formed at a position where the inserted first terminal 41 can contact the outer surface 22c of the first electrode 22. The location where the second terminal insertion hole 34 is formed may be formed at a position where the inserted second terminal 42 can contact the outer surface 23c of the second electrode 23.

[0030] Also, in the example shown in FIG. 3, the second terminal insertion hole 34 has a positioning function for ensuring that the inserted second terminal 42 comes into contact with the outer surface of the second electrode 23c. If the thickness of the second base material 31 is extremely thin compared to the length of the second terminal and the second terminal insertion hole 34 is large compared to the size of the second terminal 42, the second terminal insertion hole 34 becomes the rotation center of the second terminal 42, and there is a possibility that the second terminal 42 may not contact the second electrode 23. Therefore, in order to ensure that the second terminal 42 to be used can be positioned, the thickness of the second base material 31 and the size of the second terminal insertion hole 34 may be adjusted according to the size of the second terminal to be used.

[0031] In the example shown in FIG. 3, the first terminal insertion hole 33 is also formed in a part of the shielding portion 32 (the first shielding portion 32a). Therefore, since the first terminal 41 abuts not only on the hole portion formed in the second base material 31 portion but also on the hole portion formed in the shielding portion 32, the positioning effect is enhanced. That is, the shielding portion 32 disclosed in the present application simultaneously exhibits different effects, namely, a shielding function for preventing a user from accidentally contacting the electrode and a positioning function of the first terminal 41 connected to the power source for energizing the first electrode 22.

[0032] Note that the examples shown in FIGS. 1 to 3 are merely examples of some embodiments of the device 1. The device 1 can be variously modified within the scope of the technical idea disclosed in the present application.

[0033] (Modification Example 1 of Device 1) In the examples shown in FIGS. 1 to 3, the first electrode 22 and the second electrode 23 are substantially circular when viewed in the Z direction. Although not shown, the first electrode 22 and the second electrode 23 may be non-circular shapes, for example, polygonal shapes such as an elliptical shape, a triangular shape, a quadrangular shape, a pentagonal shape, a hexagonal shape, a heptagonal shape, an octagonal shape, a nonagonal shape, a decagonal shape, an undecagonal shape, a dodecagonal shape, etc. When the first electrode 22 and the second electrode 23 are non-circular shapes, the first electrode 22 and the second electrode 23 may be similar shapes so that the distance w when the first electrode 22 and the second electrode 23 are fixed to the first base material 21 is the same. Further, the inner shape 35 of the shielding portion 32 (the first shielding portion 32a) is similar to the outer shape of the first electrode 22 (in other words, the endless first electrode outer surface 22c), and may be larger than the outer shape of the first electrode 22 (in other words, the shape of the endless first electrode outer surface 22c when viewed in the Z direction). The device according to Modification 1 is the same as the device 1 shown in FIGS. 1 to 3 except that the shapes of the first electrode 22 and the second electrode 23 and the shielding portion 32 are different. Therefore, detailed descriptions of other components are omitted.

[0034] (Modification 2 of Device 1) In the examples shown in FIGS. 1 to 3, the first electrode 22 and the second electrode 23 are formed in an endless shape when viewed in the Z direction. Alternatively, as shown in FIG. 4, the first electrode 22 and the second electrode 23 may be formed in a substantially plate shape. FIG. 4A is a schematic top view of the electrode portion 2 constituting the device 1, FIG. 4B is a cross-sectional view taken along the arrow X-X' direction of FIG. 4A, and FIG. 4C is a cross-sectional view taken along the arrow Y-Y' direction of FIG. 4A. FIG. 5A is a schematic top view of the lid portion 3, and FIG. 5B is a cross-sectional view showing a state in which the lid portion 3 is fitted to the electrode portion 2 shown in FIG. 4B and the first terminal 41 and the second terminal 42 are inserted.

[0035] In the example shown in FIG. 4, the substantially plate-shaped first electrode 22 and second electrode 23 are fixed to the first base material 21 so as to be substantially parallel, and the first electrodes 22 and 23 arranged substantially parallel are formed at both ends thereof with a dike portion 21a made of a non-conductive material so that the sample does not flow out from the sample accommodation portion 24. Otherwise, it is the same as the electrode portion 2 shown in FIGS. 1 to 3. The dike portion 21a may be integrally formed with the first base material 21. Alternatively, the dike portion 21a may be formed separately from the first base material 21 and fixed to the first base material 21 and the first electrode 22 and the second electrode 23 in a liquid-tight manner. The fixing method may be to fix using an adhesive or a fixing member.

[0036] The shielding portion 32 of the lid portion 3 of the device 1 shown in FIGS. 1 to 3 is formed so as to shield the first electrode outer surface 22c of the first electrode 22. On the other hand, the shielding portion 32 of the device 1 according to the second modification is different from the device 1 shown in FIGS. 1 to 3 in that it is formed so as to shield at least the first electrode outer surface 22c and the second electrode outer surface 23c in order to prevent the user from contacting the first electrode 22 and the second electrode 23. In the example shown in FIG. 5, the shielding portion 32 is formed so as to shield the side surface of the dike portion 21a in addition to the first electrode outer surface 22c and the second electrode outer surface 23c. Alternatively, the shielding portion 32 may shield only the first electrode outer surface 22c and the second electrode outer surface 23c, and the second base material 31 may abut against the tip of the dike portion 21a so that the sample accommodation portion 24 is not exposed by the second base material 31 and the shielding portion 32. The device 1 according to the second modification is different from the device 1 shown in FIGS. 1 to 3 in the shapes of the first electrode 22 and the second electrode 23, and the shape and the object to be shielded of the shielding portion 32, and is the same as the device 1 shown in FIGS. 1 to 3 except that the electrode portion 2 has the dike portion 21a. Therefore, detailed description of other components is omitted.

[0037] (Modification 3 of Device 1) In the examples shown in FIGS. 1 to 3, the lid portion 3 is substantially circular when viewed in the Z direction. In other words, the second base material 31 has no holes formed therein other than the first terminal insertion hole 33 and the second terminal insertion hole 34. Alternatively, as shown in FIG. 6, a hole may be formed in the central portion of the second base material 31 of the lid portion 3. FIG. 6A is a schematic top view of the lid portion 3 according to Modification 3, and FIG. 6B is a cross-sectional view showing a state in which the lid portion 3 according to Modification 3 is fitted to the electrode portion 2 shown in FIG. 1B and the first terminal 41 and the second terminal 42 are inserted.

[0038] In the examples shown in FIGS. 6A and 6B, a hole is formed in the central portion of the second base material 31. Therefore, a second shielding portion 32b for preventing the user from contacting the second electrode outer surface 23c of the second electrode 23 is formed so as to protrude from the second base material 31. In the examples shown in FIGS. 6A and 6B, since the second electrode 23 is substantially circular, the second shielding portion 32b may be formed in a substantially circular shape having an outer shape smaller than that of the second electrode outer surface 23c. Although not shown, when the first electrode 22 and the second electrode 23 are non-circular, the second shielding portion 32b may be formed in a similar shape having an inner shape smaller than that of the second electrode outer surface 23c. The device 1 according to Modification 3 is the same as the device 1 shown in FIGS. 1 to 3 except that a hole is formed in the center of the second base material 31 of the lid portion 3 and the second shielding member 32b is formed. Therefore, detailed description of other components is omitted.

[0039] In the lid portion 3 according to Modification 3, the second terminal insertion hole 34 is also formed in a part of the second shielding portion 32b. Therefore, similar to the first shielding portion 32a, the second shielding portion 32b simultaneously exhibits different effects, namely, a shielding function for preventing the user from accidentally contacting the second electrode 23 and a positioning function for the second terminal 42.

[0040] Although illustration is omitted, a second shielding portion 32b may be additionally formed on the lid portion 3 shown in FIGS. 2 and 3. In the examples shown in FIGS. 2 and 3, since no hole is formed in the central portion of the second base material 31 of the lid portion 3, the user does not contact the second electrode 23 in a state where the lid portion 3 is fitted to the electrode portion 2. Therefore, the formation of the second shielding portion 32b is not essential. On the other hand, by forming the second shielding portion 32b on the lid portion 3 shown in FIGS. 2 and 3, the positioning function of the second terminal 42 is improved.

[0041] (Modification Example 4 of Device 1) In the examples shown in FIGS. 1 to 3, the first terminal insertion hole 33 and the second terminal insertion hole 34 are formed in the second base material 31 of the lid portion 3, and the first terminal 41 and the second terminal 42 are inserted in the Z direction. Alternatively, although illustration is omitted, the first terminal insertion hole 33 and / or the second terminal insertion hole 34 may be formed in the shielding portion 32. The device according to Modification Example 4 is the same as the device 1 shown in FIGS. 1 to 3 except that the positions where the first terminal insertion hole 33 and / or the second terminal insertion hole 34 are formed in the lid portion 3 are different. Therefore, detailed description of other components is omitted.

[0042] Note that Modification Examples 1 to 4 exemplified above are only examples of modification examples that can be adopted by Device 1, and other modification examples may also be possible. Further, two or more modification examples selected from the modification examples described in Modification Examples 1 to 4 may be combined.

[0043] Device 1 disclosed in the present application has the following effects. (1) Device 1 is composed of an electrode portion 2 including a first electrode 22 and a second electrode 23 fixed to a first base material 21 and a lid portion 3. Therefore, the structure is very simple. (2) In order to improve the connectivity with the power supply, the conventional devices described in Patent Document 1 and the like have connection terminals connected to the first electrode and the second electrode themselves. Therefore, since the electrodes are exposed and can be easily connected to the power supply, there is a risk of electric shock due to the user's carelessness. On the other hand, in the device 1 disclosed in the present application, with the electrode portion 2 shielded by the lid portion 3, the first terminal 41 and the second terminal 42 are inserted, and the structure is such that the terminals can stably contact the first electrode 22 and the second electrode 23. That is, the lid portion 3 of the present application simultaneously performs different functions: the function of shielding the electrode portion 2 from the user and the positioning function of the first terminal 41 and the second terminal 42. (3) In the embodiment where the first electrode 22 and the second electrode 23 are formed in an endless shape, the capacity of the sample accommodation portion 24 can be increased. For example, when performing cell fusion or the like at the laboratory level, a device with extremely minute parallel electrodes formed on a substrate is sufficient. On the other hand, when performing cell fusion or the like at the industrial level, it is desirable to increase the capacity of the sample accommodation portion. However, when the sample accommodation portion is enlarged, it is necessary to apply a high voltage, and the risk of electric shock increases. On the other hand, in the device 1 disclosed in the present application, with the lid portion 3 fitted to the electrode portion 2, the first terminal 41 and the second terminal 42 are connected to the first electrode 22 and the second electrode 23 respectively through insertion holes formed in the lid portion 3. Therefore, due to the configuration of the device 1 disclosed in the present application, the risk of electric shock to the user can be reduced even when a high voltage is applied. (4) When the first electrode 22 and the second electrode 23 are endless and non-circular, or when they are substantially plate-shaped and fixed to the first base material 21 so as to be substantially parallel, the lid portion 3 does not rotate with respect to the electrode portion 2 in the state where the lid portion 3 is fitted to the electrode portion 2. Therefore, the convenience of handling the device 1 is improved.

[0044] (Optional additional matters applicable to device 1) Next, with reference to FIG. 7, optional additional matters applicable to device 1 will be described. FIG. 7 is a diagram for explaining the outline of the optional additional matters, and is a schematic top view of the electrode portion 2 viewed in the Z direction. In the example shown in FIG. 7, · A first recess 22d is formed on the outer surface 22c of the first electrode to accommodate a part of the first terminal 41 inserted through the first terminal insertion hole 33. · A second recess 23d is formed on the outer surface 23c of the second electrode to accommodate a part of the second terminal 42 inserted through the second terminal insertion hole 34. · Adjust the positions of the first terminal insertion hole 33 and the second terminal insertion hole 34. Except for the above, it is the same as the embodiments shown in FIGS. 1 to 3. Therefore, since there would be overlapping descriptions, the descriptions other than the first recess 22d and the second recess 23d are omitted.

[0045] In the example shown in FIG. 7, since a part of the first terminal 41 is accommodated in the first recess 22d, there is an effect that the first terminal 41 and the first electrode 22 can be more reliably contacted. Also, since a part of the second terminal 42 is accommodated in the second recess 23d, there is an effect that the second terminal 42 and the second electrode 23 can be more reliably contacted. Although not shown, the above-described effects are achieved even when the first electrode 22 and the second electrode 23 are non-circular or substantially plate-shaped.

[0046] In addition, when the first electrode 22 and the second electrode 23 are substantially circular, in the state shown in FIG. 3B, during the use of the device 1, the lid portion 3 and the electrode portion 2 may rotate relative to each other, which may interfere with the operation. On the other hand, in the example shown in FIG. 7, since a part of the first terminal 41 inserted into the first terminal insertion hole 33 is accommodated in the first recess 22d, the first terminal 41 also functions as a stopper for preventing the electrode portion 2 and the lid portion 3 from rotating relative to each other. When using the first recess 22d or the second recess 23d from the viewpoint of being a stopper for the relative rotation of the electrode portion 2 and the lid portion 3, the effect can be achieved by using either one of the first recess 22d or the second recess 23d. Of course, both may be used. Further, in the example shown in FIG. 7, the first recess 22d is formed in the outer surface 22c of the first electrode, and the second recess 23d is formed in the outer surface 23c of the second electrode. Alternatively, although not shown, the first recess 22d may be a cylindrical recess formed in the direction of one end of the first electrode 22 from the first protruding end 22a of the first electrode 22 (in other words, the first electrode 22 covers it except for the opening formed in the first protruding end 22a), and the second recess 23d may be a cylindrical recess formed in the direction of one end of the second electrode 23 from the second protruding end 23a of the second electrode 23 (in other words, the second electrode 23 covers it except for the opening formed in the second protruding end 23a).

[0047] Needless to say, the optional additional matters shown in FIG. 7 can be added to any of the above-described embodiments of the device 1 and its modified examples.

[0048] (Embodiment of Resistance Measurement Method) Next, an embodiment of the resistance measurement method will be described. The resistance measurement method according to the embodiment is implemented using any of the above-described devices 1. The resistance measurement method includes a sample input step of inputting a liquid sample into the sample accommodation portion 24 formed between the first electrode 22 and the second electrode 23 of the electrode portion 2, a lid fitting step of fitting the lid portion 3 to the electrode portion 2, in a state where the lid portion 3 is mated with the electrode portion 3, inserting the first terminal 41 connected to the power supply 4 into the first terminal insertion hole 33 and bringing it into contact with the first electrode 22, Insert the second terminal 42 connected to the power supply 4 into the second terminal insertion hole 34 to make contact with the second electrode 23. A first terminal 41 and second terminal 42 insertion step, A voltage or current application step of applying a voltage or current to the first electrode 22 and the second electrode 23, A resistance measurement step of measuring the resistance of the liquid sample, and includes.

[0049] The liquid sample is not particularly limited as long as its resistance change can be measured by applying a voltage or current. For example, solutions such as TAE and PBS used in biochemical experiments may be used. Also, the resistance measurement step is not particularly limited as long as it can measure the resistance of the liquid sample. When a current I is applied to the sample, a resistance R and a voltage V are generated across the resistance R, and the relationship is R = voltage V / current I. As resistance measurement methods based on the above relationship, a method of applying a constant current to the sample, a method of applying a constant voltage across the sample, etc. are known. The resistance measurement device may include a power supply that can apply a constant voltage or constant current used for the resistance calculation of the above method. Known resistance measurement devices including such a power supply include, but are not limited to, Genome Editor, CUY21EDIT II, etc. manufactured by BEX Co., Ltd. As described above, when "power supply" is described in this specification, it is a concept that includes not only the literal "power supply" but also "devices including a power supply".

[0050] (Embodiment of cell fusion method) Next, an embodiment of the cell fusion method will be described. The cell fusion method according to the embodiment is implemented using any of the devices 1 described above. The cell fusion method includes A sample introduction step of introducing a sample liquid containing cells into the sample storage portion 24 formed between the first electrode 22 and the second electrode 23 of the electrode portion 2, A lid fitting step of fitting the lid portion 3 to the electrode portion 2, With the lid portion 3 fitted to the electrode portion 3, Insert the first terminal 41 connected to the power supply 4 into the first terminal insertion hole 33 to make contact with the first electrode 22, Insert the second terminal 42 connected to the power source 4 into the second terminal insertion hole 34 to make contact with the second electrode 23. A first terminal 41 and second terminal 42 insertion step, A voltage application step of applying a voltage to the first electrode 22 and the second electrode 23, A cell fusion step of fusing cells by applying a voltage, are included.

[0051] The cells are not particularly limited as long as they can be fused by applying a voltage, and known cells may be used. In the voltage application step, a voltage may be applied within a range where cell fusion is possible. Although not limited, in the case of alternating current, it is about 1 to 85 V, and in the case of direct current, it is about 1 to 3000 V. As the power source, a known power source device for cell fusion can be used. Although not limited, examples include CFB16-HB manufactured by BEX Co., Ltd.

[0052] (Embodiment of the electroporation method) Next, an embodiment of the electroporation method will be described. The electroporation method according to the embodiment is implemented using any of the devices 1 described above. The electroporation method includes A sample introduction step of introducing a sample solution containing cells and an introduction substance into the sample accommodation part 24 formed between the first electrode 22 and the second electrode 23 of the electrode part 2, A lid fitting step of fitting the lid part 3 to the electrode part 2, With the lid part 3 fitted to the electrode part 3, Insert the first terminal 41 connected to the power source 4 into the first terminal insertion hole 33 to make contact with the first electrode 22, Insert the second terminal 42 connected to the power source 4 into the second terminal insertion hole 34 to make contact with the second electrode 23, A first terminal 41 and second terminal 42 insertion step, A voltage application step of applying a voltage to the first electrode 22 and the second electrode 23, A substance introduction step of introducing the introduction substance into the cells by applying a voltage, are included.

[0053] The cells are not particularly limited as long as they can introduce substances by applying a voltage, and known cells can be used. Also, as the introduced substances, known substances such as nucleic acids and proteins can be used. In the voltage application step, a voltage may be applied within a range where the introduced substances can be introduced into the cells. Although not limited, examples of the poration pulse (Pp or PLPp) are 1 to 1000 V, and examples of the driving pulse (Pd) are 1 to 100 V. As the power source, a known electroporation power supply device can be used. Although not limited, examples include CUY21EDIT II and CUY21EX manufactured by BEX Co., Ltd.

[0054] Examples are given below to specifically describe each embodiment. However, these examples are provided only for reference of their specific aspects. These exemplifications do not limit or restrict the scope of the invention.

Example

[0055] <Example 1> [Fabrication of Device] A device was fabricated according to the following procedure. (1) Fabrication of the electrode part 2 For the first base material 21 (resin part) of the electrode part 2, polymethylpentene (Mitsui Chemicals, Inc., model number RT18) was used as the material and fabricated by injection molding. The first electrode 22 and the second electrode 23 were fabricated by cutting using stainless steel (SUS304). The first base material 21, the first electrode 22, and the second electrode 23 were screwed together with PEEK resin screws (Wilco Co., Ltd., model number PEC - 0310), and then sealed with silicone rubber (Shin-Etsu Silicone, model number KE - 441 - T). A photograph of the fabricated electrode portion 2 is shown in FIG. 8A. The first electrode 22 and the second electrode 23 were formed in a cylindrical shape, and the thickness L of the first electrode 22 and the second electrode 23 was about 5 mm. The diameter of the inner surface 22b of the first electrode 22 was about 75 mm, and the width w of the first electrode 22 and the second electrode 23 was about 5 mm. Further, as shown in FIG. 8A, a first recess 22d for accommodating a part of the first terminal 41 was formed on the outer surface 22c of the first electrode, and a second recess 23d for accommodating a part of the second terminal 42 was formed on the outer surface 23c of the second electrode.

[0056] (2) Fabrication of the lid portion 3 Polymethylpentene (Mitsui Chemicals, Inc., model number RT18) was used as the material for the lid portion 3, and it was fabricated by injection molding so as to include the first shielding portion 32a and the second shielding portion 32b. A photograph of the fabricated lid portion 3 is shown in FIG. 8B. As shown in FIG. 8B, a first terminal insertion hole 33 was formed so as to form a recess in a part of the first shielding portion 32a from the second base material 32. Further, a second terminal insertion hole 34 was formed so as to form a recess in a part of the second shielding portion 32b from the second base material 32.

[0057] [Example 2] [Performance of cell fusion] 1. Materials The materials used in the experiment were as follows. · Buffer for cell fusion: 0.3 M mannitol, 0.1 mM MgCl 2 , 0.1 mM CaCl 2 · Cell line: JKT-beta-del (JCRB0147)

[0058] 2. Experimental procedure The cell fusion experiment was conducted according to the following procedure. (1) JKT-beta-del was cultured. (2) The number of cultured cells was measured, and centrifuged at 350 × g, 5 min, 4 °C so that the number of cells became 1x10^7 cells / mL. (3) The supernatant was removed and suspended in the buffer for cell fusion. (4) Centrifugation and suspension in the buffer were repeated twice. (5) 5 ml of the cell suspension was introduced into the sample holding part formed between the first electrode and the second electrode fabricated in Example 1. (6) CFB16-HB manufactured by BEX was used as the power supply. The first terminal connected to the power supply was inserted into the first terminal insertion hole to contact the first electrode, and the second terminal connected to the power supply was inserted into the second terminal insertion hole to contact the second electrode. Cell fusion was carried out by applying a voltage under the following conditions. · ACV: 70 V, AC Time: 20 S, DCV: 1200 V, On Time: 30 μs, Off Time: 500 ms, DC cycles: 3, Post Time: 7 s, Fade: On (7) The cells were observed to confirm fusion.

[0059] Figure 9A is a photograph before voltage application, Figure 9B is a photograph after voltage application, and Figure 9C is an enlarged photograph of Figure 9B. The portion indicated by the arrow in Figure 9C shows the fused cells. From the above results, it was confirmed that cell fusion can be achieved by using the device disclosed in the present application. Although the experiment of electroporation is omitted, since cell fusion could be carried out, it is obvious without experimentation that electroporation can also be carried out.

[0060] <Example 3> [Measurement of Resistance] Next, an experiment for measuring the resistance of a liquid was conducted using the device fabricated in Example 1 according to the following procedure. 1. Sample · 10×PBS(-) (manufactured by FUJIFILM Wako Pure Chemical Corporation) · 50×TAE (manufactured by Nippon Gene Co., Ltd.)

[0061] 2. Experimental Procedure The resistance of the liquid was measured according to the following procedure. (1) Liquids with the concentrations and volumes shown in the following results were introduced into the sample holding part formed between the first electrode and the second electrode fabricated in Example 1. (2) As the resistance measurement device, Genome Editor manufactured by BEX was used. The first terminal connected to the resistance measurement device was inserted into the first terminal insertion hole to contact the first electrode, and the second terminal connected to the resistance measurement device was inserted into the second terminal insertion hole to contact the second electrode, and the resistance of the sample was measured. (3) Measurement results The concentration and the volume of the sample introduced, as well as the measured resistance values, are described below. The resistance values are the results of three measurements. a: Diluted with 1×PBS, volume 10 ml 0.002 kΩ, 0.001 kΩ, 0.001 kΩ. b: Diluted with 0.1×PBS, volume 5 ml 0.041 kΩ, 0.040 kΩ, 0.040 kΩ. c: Diluted with 0.1×PBS, volume 10 ml 0.016 kΩ, 0.016 kΩ, 0.016 kΩ. d: Diluted with 1×TAE, volume 10 ml 0.019 kΩ, 0.022 kΩ, 0.020 kΩ.

[0062] From the above results, it was confirmed that the resistance of the solution can be measured by using the device prepared in Example 1.

[0063] Generally, a device for performing cell fusion or electroporation is different from a device for measuring the resistance of a liquid. However, the device disclosed in the present application can be used for any of a resistance measurement device, a cell fusion device, and an electroporation device while ensuring safety at the lid portion.

Industrial Applicability

[0064] The device disclosed in the present application has a simple structure, can be used as a resistance measurement device, a cell fusion device, or an electroporation device, and has high safety because electric shock can be prevented. Therefore, it is useful in the fields of analysis, medicine, etc.

Explanation of Signs

[0065] 1... Device, 2... Electrode part, 21... First base material, 21a... Dam part, 22... First electrode, 22a... First protruding end, 22b... Inner surface of the first electrode, 22c... Outer surface of the first electrode, 22d... First recess, 23... Second electrode, 23a... Second protruding end, 23b... Inner surface of the second electrode, 23c... Outer surface of the second electrode, 23d... Second recess, 24... Sample accommodation part, 25... Fixing member, 26... Outer shape, 3... Cover part, 31... Second base material, 32... Shielding part, 32a... First shielding part, 32b... Second shielding part, 33... First terminal insertion hole, 34... Second terminal insertion hole, 35... Inner shape, 4... Power supply, 41... First terminal, 42... Second terminal,

Claims

1. A device for applying a voltage or current to a sample, comprising: the device includes an electrode portion and a lid portion formed of a non-conductive material; the electrode portion: a first base material formed of a non-conductive material; a first electrode; a second electrode; wherein one end of the first electrode is fixed to the first base material, and the other end of the first electrode has a first protruding end protruding from the first base material; one end of the second electrode is fixed to the first base material, and the other end of the second electrode has a second protruding end protruding from the first base material; the first electrode and the second electrode are fixed to the first base material so as not to be in contact with each other; when the lid portion is fitted to the electrode portion from the side of the first protruding end and the second protruding end, the lid portion includes: a second base material covering the first protruding end and the second protruding end; a shielding portion protruding from the second base material to prevent a user from contacting the first electrode and / or the second electrode; a first terminal insertion hole for bringing a first terminal for connecting to a power source into contact with the first electrode through the lid portion in a state where the electrode portion and the lid portion are fitted together; a second terminal insertion hole for bringing a second terminal for connecting to a power source into contact with the second electrode through the lid portion in a state where the electrode portion and the lid portion are fitted together; and the device.

2. The device according to claim 1, wherein the sample is a liquid sample, and the device is used as a resistance measuring device for measuring the resistance of the liquid sample.

3. The device according to claim 1, wherein the sample contains cells, and the device is used as a cell fusion device or an electroporation device.

4. The first electrode is formed in an endless shape except for the one end and the first protruding end; the second electrode is formed in an endless shape except for the one end and the second protruding end; in the first electrode and the second electrode fixed to the first base material, the electrode fixed on the outside is defined as the first electrode, and the electrode fixed inside the first electrode is defined as the second electrode; the space formed between the first electrode and the second electrode is defined as a sample accommodation portion; the surface of the first electrode that forms the sample accommodation portion is defined as the inner surface of the first electrode, and the surface opposite to the surface of the first electrode that forms the sample accommodation portion is defined as the outer surface of the first electrode; ​ ​ ​ When defining the surface forming the sample accommodating portion of the second electrode as the inner surface of the second electrode and the surface on the opposite side of the surface forming the sample accommodating portion of the second electrode as the outer surface of the second electrode, the shielding portion is formed to protrude from the second base material and includes a first shielding portion for preventing a user from contacting the outer surface of the first electrode The device according to claim 1.

5. The shielding portion is formed to protrude from the second base material and further includes a second shielding portion for preventing a user from contacting the outer surface of the second electrode The device according to claim 4.

6. When the first electrode and the second electrode are viewed from the first protruding end and the second protruding end sides, the first electrode and the second electrode are formed in a substantially circular shape with different diameters, the first shielding portion is formed in a substantially circular shape having an inner shape larger than the outer shape of the first electrode The device according to claim 4.

7. When the first electrode and the second electrode are viewed from the first protruding end and the second protruding end sides, the first electrode and the second electrode are formed in a non-circular similar shape, the inner shape of the first shielding portion is similar to the outer shape of the first electrode, and the inner shape is larger than the outer shape of the first electrode The device according to claim 4.

8. The first electrode and the second electrode are substantially plate-shaped and are fixed to the first base material so as to be substantially parallel, defining the space formed between the first electrode and the second electrode as a sample accommodating portion, defining the surface forming the sample accommodating portion of the first electrode as the inner surface of the first electrode and the surface on the opposite side of the surface forming the sample accommodating portion of the first electrode as the outer surface of the first electrode, When defining the surface forming the sample accommodating portion of the second electrode as the inner surface of the second electrode and the surface on the opposite side of the surface forming the sample accommodating portion of the second electrode as the outer surface of the second electrode, the shielding portion is formed to protrude from the second base material and is formed so as to prevent a user from contacting the outer surface of the first electrode and the outer surface of the second electrode The device according to claim 1.

9. A first recess is formed on the outer surface of the first electrode to accommodate a part of the first terminal inserted through the first terminal insertion hole, a second recess is formed on the outer surface of the second electrode to accommodate a part of the second terminal inserted through the second terminal insertion hole The device according to any one of claims 4 to 8.

10. A method for measuring the resistance of a liquid sample using the device according to any one of claims 1 to 2, 4 to 8, wherein the resistance measurement method comprises: A sample introduction step of introducing a liquid sample into a sample storage portion formed between a first electrode and a second electrode of an electrode portion; A lid fitting step of fitting a lid portion to the electrode portion; With the lid portion mated with the electrode portion, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage or current application step of applying a voltage or current to the first electrode and the second electrode; A resistance measurement step of measuring the resistance of the liquid sample; Including A method for measuring the resistance of a liquid sample.

11. A cell fusion method using the device according to any one of claims 1, 3 to 8, wherein the cell fusion method comprises: A sample introduction step of introducing a sample solution containing cells into a sample storage portion formed between a first electrode and a second electrode of an electrode portion; A lid fitting step of fitting a lid portion to the electrode portion; With the lid portion mated with the electrode portion, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage application step of applying a voltage to the first electrode and the second electrode; A cell fusion step of fusing cells by applying a voltage; Including A cell fusion method.

12. An electroporation method using the device according to any one of claims 1, 3 to 8, wherein the electroporation method comprises: A sample introduction step of introducing a sample solution containing cells and an introduction substance into a sample storage portion formed between a first electrode and a second electrode of an electrode portion; A lid fitting step of fitting a lid portion to the electrode portion; With the lid portion mated with the electrode portion, Inserting a first terminal connected to a power source into a first terminal insertion hole and bringing it into contact with the first electrode; Inserting a second terminal connected to a power source into a second terminal insertion hole and bringing it into contact with the second electrode, A first terminal and second terminal insertion step; A voltage application step of applying a voltage to the first electrode and the second electrode; A substance introduction step of introducing an introduction substance into cells by applying a voltage; Including An electroporation method.

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